mRNA_6999 (mRNA) Tribonema minus UTEX_B_3156

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
Namejgi.p|Trimin1|167982
Unique NamemRNA_6999
TypemRNA
OrganismTribonema minus UTEX_B_3156 (Tribonema minus UTEX_B_3156 )
Homology
BLAST of jgi.p|Trimin1|167982 vs. uniprot
Match: A0A835YQ38_9STRA (Putative cystathione gamma lyase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YQ38_9STRA)

HSP 1 Score: 319 bits (818), Expect = 2.070e-110
Identity = 159/159 (100.00%), Postives = 159/159 (100.00%), Query Frame = 1
Query:    1 MTNRLPLLPICIAARQLVHFGINPDEKTGAILTPTHQTTTYVQESVDKYLAKGYCYSRDANPTVEVLEAEIAQLENGAGACCVCTGMAATVTVMTATLKAGDHCVITDCSYGGTNRAARVMFADLAITFSFVDFRDPRIVEAAIQPNTKLIFSETPALR 477
            MTNRLPLLPICIAARQLVHFGINPDEKTGAILTPTHQTTTYVQESVDKYLAKGYCYSRDANPTVEVLEAEIAQLENGAGACCVCTGMAATVTVMTATLKAGDHCVITDCSYGGTNRAARVMFADLAITFSFVDFRDPRIVEAAIQPNTKLIFSETPALR
Sbjct:    1 MTNRLPLLPICIAARQLVHFGINPDEKTGAILTPTHQTTTYVQESVDKYLAKGYCYSRDANPTVEVLEAEIAQLENGAGACCVCTGMAATVTVMTATLKAGDHCVITDCSYGGTNRAARVMFADLAITFSFVDFRDPRIVEAAIQPNTKLIFSETPALR 159          
BLAST of jgi.p|Trimin1|167982 vs. uniprot
Match: A0A836CFW8_9STRA (Cystathionine gamma-lyase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CFW8_9STRA)

HSP 1 Score: 256 bits (654), Expect = 4.610e-82
Identity = 128/144 (88.89%), Postives = 135/144 (93.75%), Query Frame = 1
Query:   40 ARQLVHFGINPDEKTGAILTPTHQTTTYVQESVDKYLAKGYCYSRDANPTVEVLEAEIAQLENGAGACCVCTGMAATVTVMTATLKAGDHCVITDCSYGGTNRAARVMFADLAITFSFVDFRDPRIVEAAIQPNTKLIFSETPA 471
            A QLVH GI+PDEKTGAILTP +Q TTYVQESVDKYLAKG+ YSR ANPTV+ LEA+IAQLENGAGACCVCTGMAATVTVMTATLKAGDHCVITDCSYGGTNRAARVMF+DL ITFSFVDFRDP IVEAAI+PNTKLIFSETPA
Sbjct:   18 ATQLVHAGIDPDEKTGAILTPIYQATTYVQESVDKYLAKGFSYSRTANPTVKALEAKIAQLENGAGACCVCTGMAATVTVMTATLKAGDHCVITDCSYGGTNRAARVMFSDLGITFSFVDFRDPAIVEAAIRPNTKLIFSETPA 161          
BLAST of jgi.p|Trimin1|167982 vs. uniprot
Match: D8LJS6_ECTSI (Cystathionine gamma-lyase n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LJS6_ECTSI)

HSP 1 Score: 218 bits (555), Expect = 3.780e-67
Identity = 108/143 (75.52%), Postives = 127/143 (88.81%), Query Frame = 1
Query:   46 QLVHFGINPDEKTGAILTPTHQTTTYVQESVDKYLAKGYCYSRDANPTVEVLEAEIAQLENGAG-ACCVCTGMAATVTVMTATLKAGDHCVITDCSYGGTNRAARVMFADLAITFSFVDFRDPRIVEAAIQPNTKLIFSETPA 471
            +LVH GINPD++TGAILTP  Q+TT++Q+SV+KYL +G+ YSR ANPTV  LEA++A LE  AG ACCV TGMAATVTVM+A LKAGDHCVIT+CSYGGTNRAARVMF+DL +TFSFVDFRDP +V+AAI+PNTKLIFSETPA
Sbjct:   19 KLVHQGINPDKETGAILTPIVQSTTFIQDSVEKYLDRGFSYSRTANPTVAALEAKVAGLEGAAGGACCVATGMAATVTVMSAFLKAGDHCVITECSYGGTNRAARVMFSDLGVTFSFVDFRDPEVVKAAIRPNTKLIFSETPA 161          
BLAST of jgi.p|Trimin1|167982 vs. uniprot
Match: A0A7S0TAJ6_9STRA (Hypothetical protein n=1 Tax=Chrysocystis fragilis TaxID=1411660 RepID=A0A7S0TAJ6_9STRA)

HSP 1 Score: 204 bits (520), Expect = 5.970e-62
Identity = 101/142 (71.13%), Postives = 113/142 (79.58%), Query Frame = 1
Query:   46 QLVHFGINPDEKTGAILTPTHQTTTYVQESVDKYLAKGYCYSRDANPTVEVLEAEIAQLENGAGACCVCTGMAATVTVMTATLKAGDHCVITDCSYGGTNRAARVMFADLAITFSFVDFRDPRIVEAAIQPNTKLIFSETPA 471
            QLVH G+ PDE TGAILTP +Q TT+VQES+D+Y+AKGY YSR  NPTV VLE +IA LE  AGACC  TGMAATVTVM+A L  GDHCV+TDCSYGGTNR AR  F +  I FSFVDFRDP  V AAI+P TKL+FSETPA
Sbjct:   15 QLVHSGVAPDEATGAILTPIYQATTFVQESIDQYMAKGYSYSRTTNPTVRVLEQKIADLEKAAGACCFSTGMAATVTVMSAFLSQGDHCVLTDCSYGGTNRVARKQFMNYGIEFSFVDFRDPANVAAAIKPETKLVFSETPA 156          
BLAST of jgi.p|Trimin1|167982 vs. uniprot
Match: A0A0L0FZV2_9EUKA (Cystathionine gamma-synthase n=1 Tax=Sphaeroforma arctica JP610 TaxID=667725 RepID=A0A0L0FZV2_9EUKA)

HSP 1 Score: 201 bits (510), Expect = 2.250e-60
Identity = 101/147 (68.71%), Postives = 116/147 (78.91%), Query Frame = 1
Query:   31 CIAARQLVHFGINPDEKTGAILTPTHQTTTYVQESVDKYLAKGYCYSRDANPTVEVLEAEIAQLENGAGACCVCTGMAATVTVMTATLKAGDHCVITDCSYGGTNRAARVMFADLAITFSFVDFRDPRIVEAAIQPNTKLIFSETPA 471
            C  + QLVH G+NPD  TGAILTP  Q TT+VQES+D YL+KGY YSR ANPTV  LE +IA++E+ AGA C  TGMAATVTVMTA ++AGDHCVIT+CSYGGTNR AR MF  L + FSFVDF D   +E AI+PNTKLIFSETPA
Sbjct:   15 CGQSTQLVHAGVNPDPTTGAILTPIVQATTFVQESIDLYLSKGYSYSRTANPTVRALENKIAEIEHAAGATCFSTGMAATVTVMTAFMRAGDHCVITNCSYGGTNRVARKMFMGLGMEFSFVDFTDLAEIEGAIKPNTKLIFSETPA 161          
BLAST of jgi.p|Trimin1|167982 vs. uniprot
Match: A0A7S4E341_9STRA (Hypothetical protein n=3 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A7S4E341_9STRA)

HSP 1 Score: 201 bits (512), Expect = 3.800e-60
Identity = 97/141 (68.79%), Postives = 112/141 (79.43%), Query Frame = 1
Query:   49 LVHFGINPDEKTGAILTPTHQTTTYVQESVDKYLAKGYCYSRDANPTVEVLEAEIAQLENGAGACCVCTGMAATVTVMTATLKAGDHCVITDCSYGGTNRAARVMFADLAITFSFVDFRDPRIVEAAIQPNTKLIFSETPA 471
            LVH G+ PD  TG ILTP HQ+TT+VQES+D Y+AKGY YSR  NPTV VLEA++A+LE  AG+CC  TGMAATVTVM+A L  GDHCV+TDCSYGGTNRAAR +F    + FSFVDFRD   V AA +PNTKL+FSETPA
Sbjct:   73 LVHAGVAPDAATGGILTPIHQSTTFVQESIDAYMAKGYSYSRTTNPTVAVLEAKVAELEGAAGSCCFATGMAATVTVMSAFLSQGDHCVLTDCSYGGTNRAARELFGKFGVEFSFVDFRDADAVAAACRPNTKLVFSETPA 213          
BLAST of jgi.p|Trimin1|167982 vs. uniprot
Match: F0Y3F4_AURAN (Uncharacterized protein n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0Y3F4_AURAN)

HSP 1 Score: 198 bits (504), Expect = 1.570e-59
Identity = 97/142 (68.31%), Postives = 116/142 (81.69%), Query Frame = 1
Query:   46 QLVHFGINPDEKTGAILTPTHQTTTYVQESVDKYLAKGYCYSRDANPTVEVLEAEIAQLENGAGACCVCTGMAATVTVMTATLKAGDHCVITDCSYGGTNRAARVMFADLAITFSFVDFRDPRIVEAAIQPNTKLIFSETPA 471
            +LVH G++PD++TGAILTP HQ+TT+VQES+DKY+ KGY YSR  NPTV+VLEA+I+ LE+ AG  C  TGMAATVTVM+A L+ GDHCV+T+CSYGGTNR AR+ F    I FSFVDFRD   V+AAI P TKLIFSETPA
Sbjct:   17 KLVHAGVSPDKETGAILTPIHQSTTFVQESIDKYMGKGYSYSRTTNPTVKVLEAKISSLESAAGTTCFATGMAATVTVMSAFLEQGDHCVLTNCSYGGTNRVARMQFMRYGIDFSFVDFRDLAAVKAAITPKTKLIFSETPA 158          
BLAST of jgi.p|Trimin1|167982 vs. uniprot
Match: A0A7S3K336_9STRA (Hypothetical protein n=1 Tax=Aureoumbra lagunensis TaxID=44058 RepID=A0A7S3K336_9STRA)

HSP 1 Score: 194 bits (493), Expect = 6.800e-58
Identity = 98/143 (68.53%), Postives = 111/143 (77.62%), Query Frame = 1
Query:   46 QLVHFGINPDEKTGAILTPTHQTTTYVQESVDKYLAKGYCYSRDANPTVEVLEAEIAQLENGAGACCVCTGMAATVTVMTATLKAGDHCVITDCSYGGTNRAARVMFADLAITFSFVDFRDPRIVEAAIQPN-TKLIFSETPA 471
            +LVH G++PD  TGAILTP  Q TT+VQES+D+Y+AKGY Y+R  NPTV VLE ++A LEN AGACC  TGMAATVTVM+A L  GDHCV+TDCSYGGTNR AR  F    I FSFVDFRDP  VE A+ P  TKLIFSETPA
Sbjct:   15 KLVHAGVSPDPATGAILTPIVQATTFVQESIDQYMAKGYSYTRTTNPTVHVLEEKVAALENAAGACCFSTGMAATVTVMSAFLSQGDHCVLTDCSYGGTNRVARKQFMQYGIEFSFVDFRDPANVEKAVIPGKTKLIFSETPA 157          
BLAST of jgi.p|Trimin1|167982 vs. uniprot
Match: A0A7S3WA90_EMIHU (Hypothetical protein n=1 Tax=Emiliania huxleyi TaxID=2903 RepID=A0A7S3WA90_EMIHU)

HSP 1 Score: 194 bits (493), Expect = 2.240e-57
Identity = 93/142 (65.49%), Postives = 116/142 (81.69%), Query Frame = 1
Query:   46 QLVHFGINPDEKTGAILTPTHQTTTYVQESVDKYLAKGYCYSRDANPTVEVLEAEIAQLENGAGACCVCTGMAATVTVMTATLKAGDHCVITDCSYGGTNRAARVMFADLAITFSFVDFRDPRIVEAAIQPNTKLIFSETPA 471
            +LVH G++PD  TGAILTP +Q+TTYVQESVD+YLAKGY YSR  NPTV VLE +IA+LE G GA C  TGMAAT+ V+T+T++AGDHCVIT+CSYGGTNRA R +F D+ + F F+DF +   +EA ++PNTKLIFSE+PA
Sbjct:   51 KLVHAGVDPDPITGAILTPVYQSTTYVQESVDEYLAKGYSYSRTNNPTVTVLENKIAELEGGYGAACFSTGMAATIAVITSTMQAGDHCVITNCSYGGTNRACRTLFTDMGMEFDFIDFTELANIEAHLKPNTKLIFSESPA 192          
BLAST of jgi.p|Trimin1|167982 vs. uniprot
Match: A0A813F511_POLGL (Hypothetical protein n=1 Tax=Polarella glacialis TaxID=89957 RepID=A0A813F511_POLGL)

HSP 1 Score: 193 bits (490), Expect = 3.260e-57
Identity = 93/140 (66.43%), Postives = 113/140 (80.71%), Query Frame = 1
Query:   52 VHFGINPDEKTGAILTPTHQTTTYVQESVDKYLAKGYCYSRDANPTVEVLEAEIAQLENGAGACCVCTGMAATVTVMTATLKAGDHCVITDCSYGGTNRAARVMFADLAITFSFVDFRDPRIVEAAIQPNTKLIFSETPA 471
            VH G++PDE+TGA+LTP   +TT+VQESV+KYL KGY YSR  NPTV  LEA++A LENG GA C  TGMAAT + + AT+ AGDHCVI+DCSYGGTNR+ R  F+ L ++FSFVDFRDP IV  A++PNTKL+FSETPA
Sbjct:   40 VHSGVSPDERTGALLTPLFLSTTFVQESVEKYLEKGYSYSRTNNPTVSTLEAKLAVLENGYGAACFGTGMAATTSAICATMVAGDHCVISDCSYGGTNRSCREFFSHLGMSFSFVDFRDPEIVRKAMKPNTKLVFSETPA 179          
The following BLAST results are available for this feature:
BLAST of jgi.p|Trimin1|167982 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A835YQ38_9STRA2.070e-110100.00Putative cystathione gamma lyase n=1 Tax=Tribonema... [more]
A0A836CFW8_9STRA4.610e-8288.89Cystathionine gamma-lyase n=1 Tax=Tribonema minus ... [more]
D8LJS6_ECTSI3.780e-6775.52Cystathionine gamma-lyase n=2 Tax=Ectocarpus TaxID... [more]
A0A7S0TAJ6_9STRA5.970e-6271.13Hypothetical protein n=1 Tax=Chrysocystis fragilis... [more]
A0A0L0FZV2_9EUKA2.250e-6068.71Cystathionine gamma-synthase n=1 Tax=Sphaeroforma ... [more]
A0A7S4E341_9STRA3.800e-6068.79Hypothetical protein n=3 Tax=Pelagomonas calceolat... [more]
F0Y3F4_AURAN1.570e-5968.31Uncharacterized protein n=1 Tax=Aureococcus anopha... [more]
A0A7S3K336_9STRA6.800e-5868.53Hypothetical protein n=1 Tax=Aureoumbra lagunensis... [more]
A0A7S3WA90_EMIHU2.240e-5765.49Hypothetical protein n=1 Tax=Emiliania huxleyi Tax... [more]
A0A813F511_POLGL3.260e-5766.43Hypothetical protein n=1 Tax=Polarella glacialis T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Contig_54contigContig_54:2880..4412 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Tribonema minus UTEX_B_31562021-06-17
Properties
Property NameValue
ProteomeIdTrimin1|167982|e_gw1.54.236.1
Descriptioncystathionine gamma-synthase activity
COG categoryE
Max annot lvl2759|Eukaryota
EggNOG OGsCOG0626@1|root,KOG0053@2759|Eukaryota
Evalue6.2e-68
Seed ortholog2880.D8LJS6
Hectar predicted targeting categoryother localisation
TranscriptId168304
TrackFilteredModels1
ProteinId167982
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameSpeciesTypePosition
jgi.p|Trimin1|167982gene_6999Tribonema minus UTEX_B_3156 geneContig_54 2880..4412 +


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
exon_6999_1exon_6999_1Tribonema minus UTEX_B_3156 exonContig_54 2880..3074 +
exon_6999_2exon_6999_2Tribonema minus UTEX_B_3156 exonContig_54 3453..3591 +
exon_6999_3exon_6999_3Tribonema minus UTEX_B_3156 exonContig_54 4267..4412 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
CDS_6999CDS_6999Tribonema minus UTEX_B_3156 CDSContig_54 2880..3074 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
jgi.p|Trimin1|167982Trimin1|167982|e_gw1.54.236.1Tribonema minus UTEX_B_3156 polypeptideContig_54 2880..4412 +


Sequences
The following sequences are available for this feature:

spliced messenger RNA

>mRNA_6999 ID=mRNA_6999|Name=jgi.p|Trimin1|167982|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=480bp|location=Sequence derived from alignment at Contig_54:2880..4412+ (Tribonema minus UTEX_B_3156 )|Notes=Excludes all bases but those of type(s): exon.  
ATGACAAACAGATTGCCACTGCTGCCAATTTGCATTGCTGCCAGGCAGCT
GGTGCACTTCGGCATTAACCCGGATGAGAAGACCGGCGCCATTTTAACAC
CTACTCATCAGACCACCACCTATGTACAGGAGTCTGTGGACAAGTACCTC
GCCAAGGGCTACTGCTACAGCCGTGATGCTAACCCCACCGTTGAGGTGCT
GGAAGCTGAGATTGCGCAGCTGGAAAATGGCGCGGGGGCGTGCTGTGTGT
GCACTGGTATGGCTGCGACGGTGACAGTCATGACAGCGACGCTCAAGGCG
GGTGACCACTGTGTCATCACGGACTGTTCTTATGGTGGCACCAACCGAGC
GGCACGCGTTATGTTTGCAGACCTGGCCATTACGTTCAGCTTTGTCGACT
TCCGCGACCCCAGGATTGTTGAAGCGGCCATCCAGCCAAACACCAAGCTC
ATCTTCAGCGAGACGCCTGCCCTACGCTAA
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protein sequence of jgi.p|Trimin1|167982

>Trimin1|167982|e_gw1.54.236.1 ID=Trimin1|167982|e_gw1.54.236.1|Name=jgi.p|Trimin1|167982|organism=Tribonema minus UTEX_B_3156 |type=polypeptide|length=160bp
MTNRLPLLPICIAARQLVHFGINPDEKTGAILTPTHQTTTYVQESVDKYL
AKGYCYSRDANPTVEVLEAEIAQLENGAGACCVCTGMAATVTVMTATLKA
GDHCVITDCSYGGTNRAARVMFADLAITFSFVDFRDPRIVEAAIQPNTKL
IFSETPALR*
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mRNA from alignment at Contig_54:2880..4412+

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_6999 ID=mRNA_6999|Name=jgi.p|Trimin1|167982|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=1533bp|location=Sequence derived from alignment at Contig_54:2880..4412+ (Tribonema minus UTEX_B_3156 )
ATGACAAACAGATTGCCACTGCTGCCAATTTGCATTGCTGCCAGGCAGCT GGTGCACTTCGGCATTAACCCGGATGAGAAGACCGGCGCCATTTTAACAC CTACTCATCAGACCACCACCTATGTACAGGAGTCTGTGGACAAGTACCTC GCCAAGGGCTACTGCTACAGCCGTGATGCTAACCCCACCGTTGAGGTACT GCGCGCGCATCGTTTTACCTTCTTGTGGTTTTGCGCCTGCAAGTTTTCAA GGAATCCCTACCAAGCACAATTGCTGTGCTTACAAGCCATGAAGCGTTCA ATTTGTGGTAGGTGCCATCACTGCATGCATTGAACACACGTGACCACCTA CATAGCATGCACATGCACATGGACACTTGAGCAGCGACACCTGCTGCCTG TCACCAAGGGCCGCGTAAACATCACCAAAAAAACGCAAAGCACACACAAC ATGGTCAGTCGAATGTGAGAACACAATGCAACAGAGTTGTATGCGAGAGG TTATTCACAACTTTTGCACTAATCATAAATGGCGACCAAACTTTAGGGTG CTTGTCGCCCATTCACGTGCCAGGTGCTGGAAGCTGAGATTGCGCAGCTG GAAAATGGCGCGGGGGCGTGCTGTGTGTGCACTGGTATGGCTGCGACGGT GACAGTCATGACAGCGACGCTCAAGGCGGGTGACCACTGTGTCATCACGG ACTGTTCTTATGGTGAGTGCAGCTATTAGTAACCCTAAGTATGTCCTTCC CAGAACCGTTAGAAGTACTTGAATCACGGGCGATTGACTACGACACACGT GAGGGTGACAGTCATGACGGCGATTTCTACAATCAAAGATCTAACCCTAA CCGTAATTCAAGCTCCGTCCATGTGTCATTTGCAACTTGGACACTTCTCG TCGATTCAGCACACTTACCTCTCTGCCGCACATGTTGGGTGCTCCCCCTT TTCAGCATCAAGTCCAAGGCCAGCTGTCGCTGCAACCCCCTTTGCCCTTC GCGTCGCGCACCTGTGTAGATTGCACCACCACTGCTGTTCACCGAGCGGG GCCATTCCATGTGATGATGATGCCATGTAACAACCTTAATTCAGGTGCAG ACCTGCTGTACTTTGTGACTTGACAGCGTCTTTGTGTCTTCTATGATCCA CTCCAGCACCGTATCTTATCTGTAGTGAACACCTGCCAAAATTCGCTTCA ATAATGAAATCTTCTGAACCCCACCACCGGGTACTTGTTGATGTCTGCAC AACACACTTGCTTTGCAGCTTGCATGGGCCAGCGCTGAGGATGCAAACGG CGGTTTACGCTGGTATCGCTGTACCTCAAGATTATGCAAAGCTTGATGAA CACAGGAGCTGATGCTCCTTGTCGTCTCCCTGTCCAGGTGGCACCAACCG AGCGGCACGCGTTATGTTTGCAGACCTGGCCATTACGTTCAGCTTTGTCG ACTTCCGCGACCCCAGGATTGTTGAAGCGGCCATCCAGCCAAACACCAAG CTCATCTTCAGCGAGACGCCTGCCCTACGCTAA
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Coding sequence (CDS) from alignment at Contig_54:2880..4412+

>mRNA_6999 ID=mRNA_6999|Name=jgi.p|Trimin1|167982|organism=Tribonema minus UTEX_B_3156 |type=CDS|length=480bp|location=Sequence derived from alignment at Contig_54:2880..4412+ (Tribonema minus UTEX_B_3156 )
ATGACAAACAGATTGCCACTGCTGCCAATTTGCATTGCTGCCAGGCAGCT
GGTGCACTTCGGCATTAACCCGGATGAGAAGACCGGCGCCATTTTAACAC
CTACTCATCAGACCACCACCTATGTACAGGAGTCTGTGGACAAGTACCTC
GCCAAGGGCTACTGCTACAGCCGTGATGCTAACCCCACCGTTGAGGTGCT
GGAAGCTGAGATTGCGCAGCTGGAAAATGGCGCGGGGGCGTGCTGTGTGT
GCACTGGTATGGCTGCGACGGTGACAGTCATGACAGCGACGCTCAAGGCG
GGTGACCACTGTGTCATCACGGACTGTTCTTATGGTGGCACCAACCGAGC
GGCACGCGTTATGTTTGCAGACCTGGCCATTACGTTCAGCTTTGTCGACT
TCCGCGACCCCAGGATTGTTGAAGCGGCCATCCAGCCAAACACCAAGCTC
ATCTTCAGCGAGACGCCTGCCCTACGCTAA
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