mRNA_L-elsbetiae_contig13654.2779.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig13654.2779.1
Unique NamemRNA_L-elsbetiae_contig13654.2779.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
BLAST of mRNA_L-elsbetiae_contig13654.2779.1 vs. uniprot
Match: D8LQB9_ECTSI (DNA polymerase epsilon catalytic subunit n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQB9_ECTSI)

HSP 1 Score: 198 bits (503), Expect = 1.200e-56
Identity = 107/110 (97.27%), Postives = 109/110 (99.09%), Query Frame = 1
Query:    4 QVLVTTWLDEVTHMGNAHADSLLVHFYRWICARQSLLHNAALHRLVXXXXXXXFSRLIAELRRLGACVVFASFQKVIIATNKSDVKAARDYAKFVVSTVTSRDLFNTLQV 333
            +VLVTTWLDEVTHMGNAHADSLLVHFYRWICARQSLLHNA LHRLVXXXXXXXFSRLIAELRRLGACVVFASFQKVIIATNKSDVKAARDYAKFVVSTVTSRDLFNTLQ+
Sbjct:  410 KVLVTTWLDEVTHMGNAHADSLLVHFYRWICARQSLLHNATLHRLVXXXXXXXFSRLIAELRRLGACVVFASFQKVIIATNKSDVKAARDYAKFVVSTVTSRDLFNTLQL 519          
BLAST of mRNA_L-elsbetiae_contig13654.2779.1 vs. uniprot
Match: A0A6H5J9K7_9PHAE (DNA polymerase epsilon catalytic subunit n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5J9K7_9PHAE)

HSP 1 Score: 194 bits (494), Expect = 2.390e-55
Identity = 98/110 (89.09%), Postives = 101/110 (91.82%), Query Frame = 1
Query:    4 QVLVTTWLDEVTHMGNAHADSLLVHFYRWICARQSLLHNAALHRLVXXXXXXXFSRLIAELRRLGACVVFASFQKVIIATNKSDVKAARDYAKFVVSTVTSRDLFNTLQV 333
            +VLVTTWLDEVTHMGNAHADSLLVHFYRWICARQSLLHNA LH LV       FSRLIAELRRLGACVVFASFQKVIIATNKSDVKAARDYAKFV+STVTSRDLFNTLQ+
Sbjct:  986 KVLVTTWLDEVTHMGNAHADSLLVHFYRWICARQSLLHNATLHCLVHRLMHKVFSRLIAELRRLGACVVFASFQKVIIATNKSDVKAARDYAKFVISTVTSRDLFNTLQL 1095          
BLAST of mRNA_L-elsbetiae_contig13654.2779.1 vs. uniprot
Match: A0A7R9U2H8_9STRA (DNA polymerase epsilon catalytic subunit (Fragment) n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9U2H8_9STRA)

HSP 1 Score: 118 bits (295), Expect = 1.860e-30
Identity = 55/110 (50.00%), Postives = 82/110 (74.55%), Query Frame = 1
Query:    4 QVLVTTWLDEVTHMGNAHADSLLVHFYRWICARQSLLHNAALHRLVXXXXXXXFSRLIAELRRLGACVVFASFQKVIIATNKSDVKAARDYAKFVVSTVTSRDLFNTLQV 333
            ++LV+ WL E +  GN  AD++L+HFYRW+CA +S L++ ALH++V       F RL+AELRRLGA VV+ASF +++IAT K+DV +A++Y  FVV T+T+  +F+ LQ+
Sbjct:  106 RLLVSQWLAEASMNGNEIADAMLMHFYRWLCAAESKLYDPALHQMVHQLMTKVFLRLVAELRRLGAHVVYASFNRILIATQKTDVASAQEYVSFVVETITAMPVFSILQL 215          
BLAST of mRNA_L-elsbetiae_contig13654.2779.1 vs. uniprot
Match: A0A7S2MG59_9STRA (DNA polymerase epsilon catalytic subunit (Fragment) n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2MG59_9STRA)

HSP 1 Score: 113 bits (283), Expect = 3.240e-28
Identity = 57/117 (48.72%), Postives = 81/117 (69.23%), Query Frame = 1
Query:    4 QVLVTTWLDEVTHMGNAH-----ADSLLVHFYRWICARQSLLHNAALHRLVXXXXXXXFSRLIAELRRLGACVVFASFQ--KVIIATNKSDVKAARDYAKFVVSTVTSRDLFNTLQV 333
            + LV  WL+E+T  G+       AD LLVHFYRW+C+R+SLL++ AL R+V       F RL++E+RRLGA VVFA+ Q  +++I TNK+D KAARDY  F++ T+TS D+F  + +
Sbjct:  152 KTLVNQWLNEMTRKGSTKDSREVADDLLVHFYRWLCSRESLLYDPALKRVVCMLMKKIFLRLVSEIRRLGAEVVFANPQGGRIVIMTNKTDPKAARDYVNFIIETITSHDVFTHMAI 268          
BLAST of mRNA_L-elsbetiae_contig13654.2779.1 vs. uniprot
Match: W7TVX1_9STRA (DNA-directed DNA polymerase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TVX1_9STRA)

HSP 1 Score: 111 bits (277), Expect = 4.320e-26
Identity = 53/110 (48.18%), Postives = 77/110 (70.00%), Query Frame = 1
Query:    4 QVLVTTWLDEVTHMGNAHADSLLVHFYRWICARQSLLHNAALHRLVXXXXXXXFSRLIAELRRLGACVVFASFQKVIIATNKSDVKAARDYAKFVVSTVTSRDLFNTLQV 333
            +VLV TW+ EV  +G+  AD+L  +F+RW+C   SL ++ AL RL        F RL+AE+RRLGA V+FA+ Q+++IATNK D+ +A +YA FV+ TV SR +F+ LQ+
Sbjct: 1842 RVLVETWMGEVERVGSLQADALATNFWRWMCDADSLFYDPALRRLCLMLINKYFHRLVAEMRRLGAQVIFANAQRIMIATNKEDMASAHEYASFVLRTVLSRRIFSRLQI 1951          
BLAST of mRNA_L-elsbetiae_contig13654.2779.1 vs. uniprot
Match: A0A2G9GVA6_9LAMI (DNA polymerase epsilon catalytic subunit n=1 Tax=Handroanthus impetiginosus TaxID=429701 RepID=A0A2G9GVA6_9LAMI)

HSP 1 Score: 107 bits (266), Expect = 1.280e-24
Identity = 53/108 (49.07%), Postives = 75/108 (69.44%), Query Frame = 1
Query:   10 LVTTWLDEVTHMGNAHADSLLVHFYRWICARQSLLHNAALHRLVXXXXXXXFSRLIAELRRLGACVVFASFQKVIIATNKSDVKAARDYAKFVVSTVTSRDLFNTLQV 333
            L+   L +V   GN  AD++L H YRWIC+ +S LH+ ALHR++       F+ LIAELR+LGA +VFASF KVII T KSD+ AA+ Y + V+ T+ +RDLF+ +++
Sbjct:  624 LIQRCLADVITSGNVFADTILQHLYRWICSPKSKLHDPALHRMLHKVMQKIFALLIAELRKLGATIVFASFGKVIIDTGKSDISAAKAYCESVLKTLHTRDLFDLIEL 731          
BLAST of mRNA_L-elsbetiae_contig13654.2779.1 vs. uniprot
Match: A0A8M8V0N1_SESIN (DNA polymerase epsilon catalytic subunit A n=1 Tax=Sesamum indicum TaxID=4182 RepID=A0A8M8V0N1_SESIN)

HSP 1 Score: 104 bits (260), Expect = 8.460e-24
Identity = 53/111 (47.75%), Postives = 77/111 (69.37%), Query Frame = 1
Query:    1 KQVLVTTWLDEVTHMGNAHADSLLVHFYRWICARQSLLHNAALHRLVXXXXXXXFSRLIAELRRLGACVVFASFQKVIIATNKSDVKAARDYAKFVVSTVTSRDLFNTLQV 333
            KQ++     D VT  GN  AD++L H YRW+C+ +S LH+ ALHR++       F+ L+AELR+LGA +VF+SF++VII T KSD+ AA+ Y + VV T+ SRDLF  +++
Sbjct: 1761 KQLIQRCLADAVTS-GNVFADAILQHLYRWLCSPRSKLHDPALHRMLHKVMQKVFALLMAELRKLGATIVFSSFRRVIIDTGKSDLPAAKAYCESVVKTLQSRDLFEWIEL 1870          
BLAST of mRNA_L-elsbetiae_contig13654.2779.1 vs. uniprot
Match: A0A2I0JBN5_PUNGR (DNA polymerase epsilon catalytic subunit n=1 Tax=Punica granatum TaxID=22663 RepID=A0A2I0JBN5_PUNGR)

HSP 1 Score: 104 bits (259), Expect = 1.130e-23
Identity = 52/111 (46.85%), Postives = 76/111 (68.47%), Query Frame = 1
Query:    1 KQVLVTTWLDEVTHMGNAHADSLLVHFYRWICARQSLLHNAALHRLVXXXXXXXFSRLIAELRRLGACVVFASFQKVIIATNKSDVKAARDYAKFVVSTVTSRDLFNTLQV 333
            KQ++     D VT  GN +AD+LL H YRW+C+ +S LH+ ALHR++       F+ L+AELR+LGA ++FA+F KVII T K D+ AAR Y+  +V T+ +RDLF  +++
Sbjct:  687 KQLIQRCLADAVTS-GNIYADALLQHLYRWLCSPKSKLHDPALHRMLHKVMQKVFALLLAELRKLGASIIFANFSKVIIDTGKYDISAARAYSDSLVKTLQNRDLFEWIEL 796          
BLAST of mRNA_L-elsbetiae_contig13654.2779.1 vs. uniprot
Match: A0A218WMH4_PUNGR (DNA polymerase epsilon catalytic subunit n=1 Tax=Punica granatum TaxID=22663 RepID=A0A218WMH4_PUNGR)

HSP 1 Score: 104 bits (259), Expect = 1.150e-23
Identity = 52/111 (46.85%), Postives = 76/111 (68.47%), Query Frame = 1
Query:    1 KQVLVTTWLDEVTHMGNAHADSLLVHFYRWICARQSLLHNAALHRLVXXXXXXXFSRLIAELRRLGACVVFASFQKVIIATNKSDVKAARDYAKFVVSTVTSRDLFNTLQV 333
            KQ++     D VT  GN +AD+LL H YRW+C+ +S LH+ ALHR++       F+ L+AELR+LGA ++FA+F KVII T K D+ AAR Y+  +V T+ +RDLF  +++
Sbjct: 1717 KQLIQRCLADAVTS-GNIYADALLQHLYRWLCSPKSKLHDPALHRMLHKVMQKVFALLLAELRKLGASIIFANFSKVIIDTGKYDISAARAYSDSLVKTLQNRDLFEWIEL 1826          
BLAST of mRNA_L-elsbetiae_contig13654.2779.1 vs. uniprot
Match: A0A6P8CK98_PUNGR (DNA polymerase epsilon catalytic subunit n=1 Tax=Punica granatum TaxID=22663 RepID=A0A6P8CK98_PUNGR)

HSP 1 Score: 104 bits (259), Expect = 1.150e-23
Identity = 52/111 (46.85%), Postives = 76/111 (68.47%), Query Frame = 1
Query:    1 KQVLVTTWLDEVTHMGNAHADSLLVHFYRWICARQSLLHNAALHRLVXXXXXXXFSRLIAELRRLGACVVFASFQKVIIATNKSDVKAARDYAKFVVSTVTSRDLFNTLQV 333
            KQ++     D VT  GN +AD+LL H YRW+C+ +S LH+ ALHR++       F+ L+AELR+LGA ++FA+F KVII T K D+ AAR Y+  +V T+ +RDLF  +++
Sbjct: 1772 KQLIQRCLADAVTS-GNIYADALLQHLYRWLCSPKSKLHDPALHRMLHKVMQKVFALLLAELRKLGASIIFANFSKVIIDTGKYDISAARAYSDSLVKTLQNRDLFEWIEL 1881          
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig13654.2779.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LQB9_ECTSI1.200e-5697.27DNA polymerase epsilon catalytic subunit n=1 Tax=E... [more]
A0A6H5J9K7_9PHAE2.390e-5589.09DNA polymerase epsilon catalytic subunit n=1 Tax=E... [more]
A0A7R9U2H8_9STRA1.860e-3050.00DNA polymerase epsilon catalytic subunit (Fragment... [more]
A0A7S2MG59_9STRA3.240e-2848.72DNA polymerase epsilon catalytic subunit (Fragment... [more]
W7TVX1_9STRA4.320e-2648.18DNA-directed DNA polymerase n=2 Tax=Monodopsidacea... [more]
A0A2G9GVA6_9LAMI1.280e-2449.07DNA polymerase epsilon catalytic subunit n=1 Tax=H... [more]
A0A8M8V0N1_SESIN8.460e-2447.75DNA polymerase epsilon catalytic subunit A n=1 Tax... [more]
A0A2I0JBN5_PUNGR1.130e-2346.85DNA polymerase epsilon catalytic subunit n=1 Tax=P... [more]
A0A218WMH4_PUNGR1.150e-2346.85DNA polymerase epsilon catalytic subunit n=1 Tax=P... [more]
A0A6P8CK98_PUNGR1.150e-2346.85DNA polymerase epsilon catalytic subunit n=1 Tax=P... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig13654contigL-elsbetiae_contig13654:3312..4388 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score198.7
Seed ortholog evalue1.4e-48
Seed eggNOG ortholog2880.D8LQB9
Preferred namePOLE1
KEGG rclassRC02795
KEGG koko:K02324
KEGG ReactionR00375,R00376,R00377,R00378
KEGG Pathwayko00230,ko00240,ko01100,ko03030,ko03410,ko03420,ko05166,map00230,map00240,map01100,map03030,map03410,map03420,map05166
KEGG ModuleM00263
Hectar predicted targeting categoryother localisation
GOsGO:0000228,GO:0000278,GO:0003674,GO:0003676,GO:0003677,GO:0003824,GO:0003887,GO:0004518,GO:0004527,GO:0004529,GO:0004536,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005657,GO:0005694,GO:0006139,GO:0006259,GO:0006260,GO:0006261,GO:0006271,GO:0006272,GO:0006281,GO:0006284,GO:0006287,GO:0006289,GO:0006297,GO:0006725,GO:0006807,GO:0006950,GO:0006974,GO:0007049,GO:0008150,GO:0008152,GO:0008296,GO:0008297,GO:0008310,GO:0008408,GO:0008622,GO:0009058,GO:0009059,GO:0009987,GO:0016070,GO:0016740,GO:0016772,GO:0016779,GO:0016787,GO:0016788,GO:0016796,GO:0016895,GO:0018130,GO:0019438,GO:0022616,GO:0031974,GO:0031981,GO:0032991,GO:0033554,GO:0034061,GO:0034641,GO:0034645,GO:0034654,GO:0042575,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044271,GO:0044422,GO:0044424,GO:0044427,GO:0044428,GO:0044446,GO:0044454,GO:0044464,GO:0045004,GO:0045005,GO:0046483,GO:0050896,GO:0051716,GO:0061695,GO:0070013,GO:0071704,GO:0071897,GO:0090304,GO:0090305,GO:0097159,GO:0140097,GO:1901360,GO:1901362,GO:1901363,GO:1901576,GO:1902494,GO:1990234
EggNOG free text desc.DNA replication proofreading
EggNOG OGsCOG0417@1,KOG1798@2759
EC2.7.7.7
COG Functional cat.L
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko03032,ko03400
Exons3
Model size342
Cds size342
Stop1
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig13654.2779.1prot_L-elsbetiae_contig13654.2779.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig13654 3312..4388 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815530.9441428-CDS-L-elsbetiae_contig13654:3311..33971622815530.9441428-CDS-L-elsbetiae_contig13654:3311..3397Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig13654 3312..3397 +
1691682943.499146-CDS-L-elsbetiae_contig13654:3311..33971691682943.499146-CDS-L-elsbetiae_contig13654:3311..3397Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig13654 3312..3397 +
1622815530.9585748-CDS-L-elsbetiae_contig13654:3680..37641622815530.9585748-CDS-L-elsbetiae_contig13654:3680..3764Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig13654 3681..3764 +
1691682943.5117066-CDS-L-elsbetiae_contig13654:3680..37641691682943.5117066-CDS-L-elsbetiae_contig13654:3680..3764Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig13654 3681..3764 +
1622815530.969367-CDS-L-elsbetiae_contig13654:4216..43881622815530.969367-CDS-L-elsbetiae_contig13654:4216..4388Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig13654 4217..4388 +
1691682943.520173-CDS-L-elsbetiae_contig13654:4216..43881691682943.520173-CDS-L-elsbetiae_contig13654:4216..4388Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig13654 4217..4388 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig13654.2779.1

>prot_L-elsbetiae_contig13654.2779.1 ID=prot_L-elsbetiae_contig13654.2779.1|Name=mRNA_L-elsbetiae_contig13654.2779.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=114bp
KQVLVTTWLDEVTHMGNAHADSLLVHFYRWICARQSLLHNAALHRLVHRL
MHKVFSRLIAELRRLGACVVFASFQKVIIATNKSDVKAARDYAKFVVSTV
TSRDLFNTLQVFC*
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mRNA from alignment at L-elsbetiae_contig13654:3312..4388+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig13654.2779.1 ID=mRNA_L-elsbetiae_contig13654.2779.1|Name=mRNA_L-elsbetiae_contig13654.2779.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=1077bp|location=Sequence derived from alignment at L-elsbetiae_contig13654:3312..4388+ (Laminarionema elsbetiae ELsaHSoW15)
AAACAGGTTCTGGTGACCACGTGGCTGGACGAGGTGACCCACATGGGCAA CGCGCACGCGGACAGCCTTCTCGTGCACTTCTACCGGTGAGAGGGGAGAC GGGGGGAAATACAAATCGTTCGTTTGTTTGTGTGTAACATACACATGTGT GTCACAGCGCCGTCTACCAGCTTTGCGGTGATGATGATGATGATGTTATG ATAGCTCCAAAGGTATGCGCCGGTGCTGATTTCTGAGCGCTCTTTGTTTT GTGCTCTTCGTGCTTGGTTTCGTCGGCCCCGTCCCCTCCCTCGCCCCCTC GTGCCCTCCGTCGAAAACCTTGGTTTTTGCCGTCGGAATGTCGCGAAAAA TAAATGAAACGCCGAACAGGTGGATCTGTGCTCGCCAGTCTTTGCTGCAC AACGCCGCGCTCCACCGGCTGGTGCACCGACTCATGCACAAGGTCTTCAG CAGGTCCGCGGGTGTTTTATGCTTTGTCTGTGTATGTATGTACAAGCGGG ATTAAAAACCCCGAACTTTTTTTTTTTGTTAGCTGCAAGTGTCAATCACG CCCTCGTTTCCTTTTCACCGAAAGGGAGAGAGAGATAACAGAAGGAGATG AGAGGCAAAGAGAAATGGGGACAGAGAGAGAGAGGGAGAGAGAGAAAAGG GTTGAGAGACAAGAGAGAAAAGGAGAGGAGCGGCAAAGATAATAGAGAGA GAGAGAGAGAGATAGAGAAGGGTTGGGAGACGGAGAGCCAGAGAGCCAGA GAGCCAGAGAGAGGCGTCTTGTCACCGCGCCTTGGCTGTTTTCTGTTCGC AAAATGGCGGTGCCGCACATAGCTTTCGTGGTCACGTTTTCGCCCCTAAC CTGTGTACTGCAACTGTGTATCATCGTGCACCCCGCACCAACATGAAACC GTCAGGCTGATCGCCGAGCTGCGCCGCCTGGGGGCGTGCGTGGTGTTCGC GAGCTTCCAGAAGGTGATTATCGCGACCAACAAGTCGGACGTGAAGGCGG CGAGGGACTATGCCAAGTTCGTCGTGTCCACCGTCACCTCCCGGGACCTC TTCAACACGCTGCAGGTATTTTGCTGA
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Coding sequence (CDS) from alignment at L-elsbetiae_contig13654:3312..4388+

>mRNA_L-elsbetiae_contig13654.2779.1 ID=mRNA_L-elsbetiae_contig13654.2779.1|Name=mRNA_L-elsbetiae_contig13654.2779.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=684bp|location=Sequence derived from alignment at L-elsbetiae_contig13654:3312..4388+ (Laminarionema elsbetiae ELsaHSoW15)
AAACAGGTTCTGGTGACCACGTGGCTGGACGAGGTGACCCACATGGGCAA
CGCGCACGCGGACAGCCTTCTCGTGCACTTCTACCGAAACAGGTTCTGGT
GACCACGTGGCTGGACGAGGTGACCCACATGGGCAACGCGCACGCGGACA
GCCTTCTCGTGCACTTCTACCGGTGGATCTGTGCTCGCCAGTCTTTGCTG
CACAACGCCGCGCTCCACCGGCTGGTGCACCGACTCATGCACAAGGTCTT
CAGCAGGTGGATCTGTGCTCGCCAGTCTTTGCTGCACAACGCCGCGCTCC
ACCGGCTGGTGCACCGACTCATGCACAAGGTCTTCAGCAGGCTGATCGCC
GAGCTGCGCCGCCTGGGGGCGTGCGTGGTGTTCGCGAGCTTCCAGAAGGT
GATTATCGCGACCAACAAGTCGGACGTGAAGGCGGCGAGGGACTATGCCA
AGTTCGTCGTGTCCACCGTCACCTCCCGGGACCTCTTCAACACGCTGCAG
GTATTTTGCTGAGCTGATCGCCGAGCTGCGCCGCCTGGGGGCGTGCGTGG
TGTTCGCGAGCTTCCAGAAGGTGATTATCGCGACCAACAAGTCGGACGTG
AAGGCGGCGAGGGACTATGCCAAGTTCGTCGTGTCCACCGTCACCTCCCG
GGACCTCTTCAACACGCTGCAGGTATTTTGCTGA
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