mRNA_H-elongata_contig1309.2236.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig1309.2236.1
Unique NamemRNA_H-elongata_contig1309.2236.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig1309.2236.1 vs. uniprot
Match: A0A6H5JW68_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JW68_9PHAE)

HSP 1 Score: 260 bits (664), Expect = 1.240e-78
Identity = 122/220 (55.45%), Postives = 162/220 (73.64%), Query Frame = 3 
Query:  615 PKCMYSSAPDELNFAIHVRLGDRATFLNGFETGYVHHIATFIETVTAAVTRQGLDVPMFHIFSETLMPCPDVQSRTFPEFPNWPVEKYQIEPCLDPVTPVDCAEKRAGALSCNPERSGIFRVFGKPLYLHVGLDVQNDLTCMSQADGVLMGCSTFGQISGVLNKGLKFFSFECAGAKTPNHYKMMPPLAVAEQGNLWVPMAGSWHDPSILSEAIFEVALD 1274
            P+C +S +  +L+ AIHVRLGDR   LN  +  Y  ++  F+ETV +A   +G D P+FHIFSET   CP  ++  F EFP WPVE  Q+  C++  TP +C EK AGA SCNP RSGIF V G+ + LHVGLDVQNDL+CM+ A+GVLMGCSTFGQ++GVL+KG++FFS ECAG +T   YK++PP+A+A +G++WVP++GSWHDP I SE+I   +LD
Sbjct:   68 PECAFSQS--KLDIAIHVRLGDRKDSLN--DDSYFDYLEDFMETVASAAIARGQDHPVFHIFSETHSRCPSAENGAFEEFPKWPVEMDQVSTCMEATTPEECPEKDAGAPSCNPTRSGIFAVDGREMNLHVGLDVQNDLSCMANANGVLMGCSTFGQVAGVLSKGIRFFSTECAGWRTTIQYKLIPPMAIAGRGHMWVPVSGSWHDPVIASESILGASLD 283
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BLAST of mRNA_H-elongata_contig1309.2236.1 vs. uniprot
Match: D7G5J8_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G5J8_ECTSI)

HSP 1 Score: 239 bits (609), Expect = 1.190e-71
Identity = 111/207 (53.62%), Postives = 144/207 (69.57%), Query Frame = 3 
Query:  672 LGDRATFLNGFETGYVHHIATFIETVTAAVTRQGLDVPMFHIFSETLMPCPDVQSRTFPEFPNWPVEKYQIEPCLDPVTPVDCAEKRAGALSCNPERSGIFRVFGKPLYLHVGLDVQNDLTCMSQADGVLMGCSTFGQISGVLNKGLKFFSFECAGAKTPNHYKMMPPLAVAEQGNLWVPMAGSWHDPSILSEAIFEVALDNHLKNK 1292
            +GDR  F +G    Y H++   +ETV      +GL  P+FH+FSETL PCP   +  F EFP WPVE  +IE CL   TP+DC EK+AG   C P+R+GIFR+  K + LHVG DVQN+++CM QADGVLMGCSTFGQI+G+L +G+  FS  C+G  TP+ Y+ +PPLA+AE+G+LWVP+ GSWHDP + S +IF  ALD  L  K
Sbjct:    1 MGDRRAFYDG-SLQYFHNVELIMETVADEFRERGLPEPLFHVFSETLSPCPSTVTGLFDEFPIWPVELDKIEECLAAATPIDCPEKQAGTF-CTPKRAGIFRIRDKSIVLHVGSDVQNEMSCMIQADGVLMGCSTFGQIAGLLTRGISLFSTRCSGEATPHQYRSIPPLAIAEKGHLWVPIEGSWHDPVLTSRSIFSAALDTLLVAK 205
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BLAST of mRNA_H-elongata_contig1309.2236.1 vs. uniprot
Match: A0A6H5L814_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L814_9PHAE)

HSP 1 Score: 209 bits (531), Expect = 1.290e-59
Identity = 113/242 (46.69%), Postives = 145/242 (59.92%), Query Frame = 3 
Query:  591 ESIRFNHLPKCMYSSAPDELNFAIHVRLGDRATFLNGFETGYVHHIATFIETVTAAVTRQGLDVPMFHIFSETLMPCPDVQSRTFPEFPNWPVEKYQIEPCLD-------PVTPVDCAEKRAGALSCNPERSGIFRVFGKPLYLHVGLDVQNDLTCMSQADGVLMGCSTFGQISGVLNKG-LKFFSFECAGAKTPNHYKMMPPLAVAEQGNLWVPMAGSWHDPSILSEAIFEVALDNHLKNK 1292
            ESIR+N  P C Y  +  +LNFA+HVR+GDR   L G    Y   +  F+ TVT AV  +    PMFH+FSE L PCP  ++ TF EFP WPVE+ Q++  +            V    + A        R G FRV G+P+ LH+G +    L+CM  ADG+LMGCSTFGQ++GVLN   + FFS EC G   P   KM+PPLAVAE+G +WVP+AGSW DP + ++ IF  ALD  LK K
Sbjct:   10 ESIRYNDEPGCGYEES--QLNFALHVRMGDRRE-LEGVTPEYFSLLEDFMATVTQAVVEREQAAPMFHVFSEALYPCPSPENATFTEFPLWPVERDQVDLNVSLHRLFSLSNEKVSVCLRNAAEQGAPASRKGTFRVEGRPVLLHLGRNAAEALSCMVHADGLLMGCSTFGQLAGVLNGDRISFFSTECGGHGAPLQSKMIPPLAVAERGRMWVPIAGSWRDPVLRAKHIFSAALDALLKRK 248
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BLAST of mRNA_H-elongata_contig1309.2236.1 vs. uniprot
Match: D7FRT1_ECTSI (Long-chain acyl-CoA synthetase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FRT1_ECTSI)

HSP 1 Score: 209 bits (532), Expect = 1.090e-53
Identity = 112/235 (47.66%), Postives = 146/235 (62.13%), Query Frame = 3 
Query:  603 FNHLPKCMYSSAPDELNFAIHVRLGDRATFLNGFETGYVHHIATFIETVTAAVTRQGLDVPMFHIFSETLMPCPDVQSRTFPEFPNWPVEKYQIEPCLDPVTPVDCAEKRAGALSCNPERSGIFRVFGKPLYLHVGLDVQNDLTCMSQADGVLMGCSTFGQISGVLN-KGLKFFSFECAGAKTPNHYKMMPPLAVAEQGNLWVPMAGSWHDPSILSEAIFEVALDNHLKNKRVSS 1304
            +N  P+C Y  +  ELNFA+HVR+GDR   L G    Y   +  F+ TVT AV  +    PMFH+FSE L PCP  ++ TF EFP WPVE+ Q+  C     P + AE+   A      R   FRV G+P+ LH+G +    L+CM  ADG+LMGCSTFGQ++GVLN  G+ FFS EC G   P   KM+PPLAVAE+G +WVP+AGSW DP + ++ +F   LD  LK K+ S+
Sbjct:  172 YNDEPECGYEES--ELNFALHVRMGDRRE-LEGVTPEYFSLLEGFMATVTQAVVEREQAAPMFHVFSEALYPCPFPENATFTEFPFWPVERDQVSVC-----PRNAAEQDGPA-----SRPDTFRVEGRPVLLHLGRNAAEALSCMVHADGLLMGCSTFGQLAGVLNGDGISFFSTECGGHGAPLQSKMIPPLAVAERGRMWVPIAGSWRDPVLRAKHVFSAVLDELLKRKQGST 393
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BLAST of mRNA_H-elongata_contig1309.2236.1 vs. uniprot
Match: A0A6H5JXM8_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JXM8_9PHAE)

HSP 1 Score: 172 bits (436), Expect = 1.050e-46
Identity = 82/185 (44.32%), Postives = 112/185 (60.54%), Query Frame = 3 
Query:  738 IETVTAAVTRQGLDVPMFHIFSETLMPCPDVQSRTFPEFPNWPVEKYQIEPCLDPVTPVDCAEKRAGALSCNPERSGIFRVFGKPLYLHVGLDVQNDLTCMSQADGVLMGCSTFGQISGVLNKGLKFFSFECAGAKTPNHYKMMPPLAVAEQGNLWVPMAGSWHDPSILSEAIFEVALDNHLKNK 1292
            ++ +   V   G   P+FHIFSET +PCP ++   FPEFP W V   +I  C     P DC EK+AG   C P  +GIFRV G+P+  +VG +V   L  M  AD V+MGCS FGQ++G+L+KG+K FS  C G  T    KM P  A+AE G LWVP+ GSW DP++ +  IF  A + +++ +
Sbjct:    1 MKVIAKEVHGLGQRTPLFHIFSETELPCPSLEDGVFPEFPEWAVAPDEIRECHSARPPDDCPEKQAGGDFCYPHHTGIFRVPGEPIIFNVGGNVVQSLMSMISADAVVMGCSAFGQLAGILSKGIKLFSTGCEGNMTWEQNKMAPLFAIAELGELWVPLEGSWADPTLTNVGIFRKAFELYVERE 185
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BLAST of mRNA_H-elongata_contig1309.2236.1 vs. uniprot
Match: A0A6H5JJ94_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JJ94_9PHAE)

HSP 1 Score: 110 bits (276), Expect = 4.150e-25
Identity = 59/138 (42.75%), Postives = 77/138 (55.80%), Query Frame = 3 
Query:  879 QIEPCLDPVTPVDCAEKRAGALSCNPERSGIFRVFGKPLYLHVGLDVQNDLTCMSQADGVLMGCSTFGQISGVLNKGLKFFSFECAGAKTPNHYKMMPPLAVAEQGNLWVPMAGSWHDPSILSEAIFEVALDNHLKNK 1292
            QIE CL   TP DC EK+AG   C P+R+GIFR+  K + LH+G DVQN+++C+ +ADGVLM                                + +PPL +AE+G+LWVP+ GSWHDP   S +IF  ALD  L  K
Sbjct:   21 QIEECLAATTPQDCPEKQAGTF-CTPKRAGIFRIRYKSIVLHMGSDVQNEMSCIIEADGVLM--------------------------------RSIPPLTIAEKGHLWVPIEGSWHDPVWSSRSIFSAALDTLLVAK 125
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig1309.2236.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 6
Match NameE-valueIdentityDescription
A0A6H5JW68_9PHAE1.240e-7855.45Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7G5J8_ECTSI1.190e-7153.62Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5L814_9PHAE1.290e-5946.69Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7FRT1_ECTSI1.090e-5347.66Long-chain acyl-CoA synthetase n=1 Tax=Ectocarpus ... [more]
A0A6H5JXM8_9PHAE1.050e-4644.32Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5JJ94_9PHAE4.150e-2542.75Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig1309contigH-elongata_contig1309:14829..16858 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score204.9
Seed ortholog evalue4e-50
Seed eggNOG ortholog2880.D7FRT1
EggNOG free text desc.fatty acid biosynthetic process
EggNOG OGsCOG0318@1,KOG3628@2759
COG Functional cat.IQ
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Hectar predicted targeting categoryother localisation
Exons2
Model size1868
Cds size693
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig1309.2236.1prot_H-elongata_contig1309.2236.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig1309 15381..16235 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929777.6972404-UTR-H-elongata_contig1309:14828..153801622929777.6972404-UTR-H-elongata_contig1309:14828..15380Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig1309 14829..15380 -
1691679153.96185-UTR-H-elongata_contig1309:14828..153801691679153.96185-UTR-H-elongata_contig1309:14828..15380Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig1309 14829..15380 -
1622929777.7720659-UTR-H-elongata_contig1309:16235..168581622929777.7720659-UTR-H-elongata_contig1309:16235..16858Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig1309 16236..16858 -
1691679153.9953086-UTR-H-elongata_contig1309:16235..168581691679153.9953086-UTR-H-elongata_contig1309:16235..16858Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig1309 16236..16858 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929777.7443228-CDS-H-elongata_contig1309:15380..158151622929777.7443228-CDS-H-elongata_contig1309:15380..15815Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig1309 15381..15815 -
1691679153.9749796-CDS-H-elongata_contig1309:15380..158151691679153.9749796-CDS-H-elongata_contig1309:15380..15815Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig1309 15381..15815 -
1622929777.761605-CDS-H-elongata_contig1309:15977..162351622929777.761605-CDS-H-elongata_contig1309:15977..16235Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig1309 15978..16235 -
1691679153.9843154-CDS-H-elongata_contig1309:15977..162351691679153.9843154-CDS-H-elongata_contig1309:15977..16235Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig1309 15978..16235 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig1309.2236.1

>prot_H-elongata_contig1309.2236.1 ID=prot_H-elongata_contig1309.2236.1|Name=mRNA_H-elongata_contig1309.2236.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=231bp
MYSSAPDELNFAIHVRLGDRATFLNGFETGYVHHIATFIETVTAAVTRQG
LDVPMFHIFSETLMPCPDVQSRTFPEFPNWPVEKYQIEPCLDPVTPVDCA
EKRAGALSCNPERSGIFRVFGKPLYLHVGLDVQNDLTCMSQADGVLMGCS
TFGQISGVLNKGLKFFSFECAGAKTPNHYKMMPPLAVAEQGNLWVPMAGS
WHDPSILSEAIFEVALDNHLKNKRVSSNKE*
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mRNA from alignment at H-elongata_contig1309:14829..16858-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig1309.2236.1 ID=mRNA_H-elongata_contig1309.2236.1|Name=mRNA_H-elongata_contig1309.2236.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=2030bp|location=Sequence derived from alignment at H-elongata_contig1309:14829..16858- (Himanthalia elongata Himel1 dioecious)
AAAAGAAGTAGACCGTTGGTACCTAGGATAAGTAATGAAAAATGATGATA
ATAATAATAATGCTGCTAAAATATGTAAATATAATGAATGAAGATTACAT
CACTGCAGTTTCCTTTTGCATGGAGAGTATACTGTAAGTTTTCTTTCCTG
ATGGTGTTTTTTCTACTTTCATTAACAATTTCAATCAAAAACATATTTAG
TCTCATTTTCTACGGAAACCTTCCGTGAACTTTTATCTCCTAGCGCCAAA
TGTCTGGCTATCAAATATTAATGATTTCGACGATGCGAATTCAAATAGGT
GAAATTGTTTGTCACATACCCCATCACTGCAGCACCGTGGTGATATCCCT
GATAGGCTGCGAAATACCACCGCAAAGCCCCACCAGAAATGACGCAATCT
AGGAACGGGGAGAAGCTGTTGCTGCCGGGACTGCCATTAAATATTGATGT
TACCTGATCTAAAAAGACCTTGAACGATTGTCCTGTGATATCAGTCTCAG
ATATCCAGAGTCCTGGACGCTTCCTGATGTCTATATAATATTACCCCCAT
TAAGTTGCTGCATGTCTCGACCCTGTACCTTGTGTGGTTGGAGTCTATCA
GGTTCAACCATCTGCCTAAGTGTATGTACTCTTCGGCACCTGACGAGCTC
AATTTTGCAATCCACGTGAGACTCGGGGATAGGGCAACATTTCTGAATGG
GTTTGAAACAGGCTACGTACACCACATCGCCACCTTCATCGAGACGGTGA
CCGCAGCTGTAACGCGGCAAGGGCTTGACGTGCCAATGTTCCACATTTTC
TCGGAGACACTAATGCCGTGCCCAGACGTACAATCCAGAACGTTTCCGGA
GTTCCCGAACTGGCCAGTTGAGAAGTATCAGGTGCGGCATAACGTTTATG
GTTCCACATGATCCGATGAATCACATCTTTATGTGGATGAATTGGGTACA
TTTTAGCAGAGGCCTTGTAGACAGCATAAAATACGAAGTGTGAAATATTT
TTTCCTAACGCTGTGTTTTTTCCCGCCCCCTGGAACTCTGCAGATAGAAC
CGTGCCTGGACCCAGTAACACCGGTGGACTGTGCGGAAAAACGGGCTGGG
GCGCTGAGTTGCAACCCGGAGCGATCGGGAATATTCCGTGTGTTTGGGAA
GCCTTTGTACCTCCACGTCGGACTTGACGTACAAAACGATCTCACTTGTA
TGTCTCAGGCTGATGGAGTTCTAATGGGCTGCTCGACGTTTGGTCAGATC
TCTGGAGTTCTCAACAAGGGGCTAAAATTCTTCTCCTTCGAATGTGCAGG
AGCAAAAACCCCGAATCACTATAAAATGATGCCTCCTCTCGCCGTTGCTG
AGCAAGGGAATCTTTGGGTTCCTATGGCAGGATCGTGGCATGACCCCTCG
ATATTGTCCGAAGCAATCTTCGAGGTGGCATTAGACAATCATCTGAAAAA
CAAGCGAGTTTCTTCAAATAAGGAATAACAGTAACAACACAAGCTAAATT
ATTTTTGACGGAGTCTCACACCTCGCAGCAATCGGTGGGCTGTGCTGTAC
TGGATGAGTAGGAAGGCTTGAAGGCTTCTGGAACACGTTTTGTTTCTTTC
GAAGGGTATAATAAACTATCAATATATATGCGGTTGGTCGGCTCCCGTGT
ACATATCTATTTAATTTTGTCTCATCGTTAAGCTTGGTTCTCCTCCGGGT
TCGAGACGATTCGTCCTTTGTCCGGATAAGATTGGATCTCCTGTGGTTCA
TGTGATACTGGATTAGATCTGATACAAAGGTTTTCTCTAAGATTGTCTCG
CAGGGCGTACTATGCACATGCGGTTTTATGTGTGACTCTGATTTCGCTGC
CAGATGTTTAGTGAAAGGGCGCACGCGCTCGTTCATGTTGTAGTGCTATG
GGGAGAGTACAGAATAAAAATATTAAGCTATGGTAGGAGCACGATCTGCA
GAATGTTTTATTATAGTCTAGCTATACCACCAGGTGTTAGTACTTGATAG
TGCAGTTCATGTGTTGTGCCGAGTAAAGCA
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Coding sequence (CDS) from alignment at H-elongata_contig1309:14829..16858-

>mRNA_H-elongata_contig1309.2236.1 ID=mRNA_H-elongata_contig1309.2236.1|Name=mRNA_H-elongata_contig1309.2236.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=1386bp|location=Sequence derived from alignment at H-elongata_contig1309:14829..16858- (Himanthalia elongata Himel1 dioecious)
ATGTACTCTTCGGCACCTGACGAGCTCAATTTTGCAATCCACGTGAGACT
CGGGGATAGGGCAACATTTCTGAATGGGTTTGAAACAGGCTACGTACACC
ACATCGCCACCTTCATCGAGACGGTGACCGCAGCTGTAACGCGGCAAGGG
CTTGACGTGCCAATGTTCCACATTTTCTCGGAGACACTAATGCCGTGCCC
AGACGTACAATCCAGAACGTTTCCGGAGTTCCCGAACTGGCCAGTTGAGA
AGTATCAGATGTACTCTTCGGCACCTGACGAGCTCAATTTTGCAATCCAC
GTGAGACTCGGGGATAGGGCAACATTTCTGAATGGGTTTGAAACAGGCTA
CGTACACCACATCGCCACCTTCATCGAGACGGTGACCGCAGCTGTAACGC
GGCAAGGGCTTGACGTGCCAATGTTCCACATTTTCTCGGAGACACTAATG
CCGTGCCCAGACGTACAATCCAGAACGTTTCCGGAGTTCCCGAACTGGCC
AGTTGAGAAGTATCAGATAGAACCGTGCCTGGACCCAGTAACACCGGTGG
ACTGTGCGGAAAAACGGGCTGGGGCGCTGAGTTGCAACCCGGAGCGATCG
GGAATATTCCGTGTGTTTGGGAAGCCTTTGTACCTCCACGTCGGACTTGA
CGTACAAAACGATCTCACTTGTATGTCTCAGGCTGATGGAGTTCTAATGG
GCTGCTCGACGTTTGGTCAGATCTCTGGAGTTCTCAACAAGGGGCTAAAA
TTCTTCTCCTTCGAATGTGCAGGAGCAAAAACCCCGAATCACTATAAAAT
GATGCCTCCTCTCGCCGTTGCTGAGCAAGGGAATCTTTGGGTTCCTATGG
CAGGATCGTGGCATGACCCCTCGATATTGTCCGAAGCAATCTTCGAGGTG
GCATTAGACAATCATCTGAAAAACAAGCGAGTTTCTTCAAATAAGGAATA
AATAGAACCGTGCCTGGACCCAGTAACACCGGTGGACTGTGCGGAAAAAC
GGGCTGGGGCGCTGAGTTGCAACCCGGAGCGATCGGGAATATTCCGTGTG
TTTGGGAAGCCTTTGTACCTCCACGTCGGACTTGACGTACAAAACGATCT
CACTTGTATGTCTCAGGCTGATGGAGTTCTAATGGGCTGCTCGACGTTTG
GTCAGATCTCTGGAGTTCTCAACAAGGGGCTAAAATTCTTCTCCTTCGAA
TGTGCAGGAGCAAAAACCCCGAATCACTATAAAATGATGCCTCCTCTCGC
CGTTGCTGAGCAAGGGAATCTTTGGGTTCCTATGGCAGGATCGTGGCATG
ACCCCTCGATATTGTCCGAAGCAATCTTCGAGGTGGCATTAGACAATCAT
CTGAAAAACAAGCGAGTTTCTTCAAATAAGGAATAA
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