mRNA_Ecto-sp13_S_contig19069.6069.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig19069.6069.1
Unique NamemRNA_Ecto-sp13_S_contig19069.6069.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig19069.6069.1 vs. uniprot
Match: D7FSV8_ECTSI (Sister chromatid cohesion protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FSV8_ECTSI)

HSP 1 Score: 203 bits (516), Expect = 1.650e-58
Identity = 98/103 (95.15%), Postives = 99/103 (96.12%), Query Frame = 3 
Query:    3 VMMLDCDRCHRWFHGHCVGITSQEDCPEEWFCVDCTILRQVSAQKHAHAKRQSMDAASHDLTKEFGDEAAGSDDEGAGGGGKVVAFEVTDLDVFRQLVLNRLT 311
            VMMLDCDRCHRWFHGHCVGITSQEDCPEEWFCVDCTILRQVSAQKHAHAKRQS+DAASHDLTKE GDE AGSDDEG  GGGKVVAFEVTDLDVFRQLVLNRLT
Sbjct:  958 VMMLDCDRCHRWFHGHCVGITSQEDCPEEWFCVDCTILRQVSAQKHAHAKRQSLDAASHDLTKELGDE-AGSDDEGGVGGGKVVAFEVTDLDVFRQLVLNRLT 1059
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BLAST of mRNA_Ecto-sp13_S_contig19069.6069.1 vs. uniprot
Match: A0A6H5JLU6_9PHAE (PHD-type domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JLU6_9PHAE)

HSP 1 Score: 174 bits (440), Expect = 2.810e-48
Identity = 87/103 (84.47%), Postives = 88/103 (85.44%), Query Frame = 3 
Query:    3 VMMLDCDRCHRWFHGHCVGITSQEDCPEEWFCVDCTILRQVSAQKHAHAKRQSMDAASHDLTKEFGDEAAGSDDEGAGGGGKVVAFEVTDLDVFRQLVLNRLT 311
            VMMLDCDRCHRWFHG CVGITSQEDCPEEWFCVDCTILRQV+AQK AHA RQS          EFGDE AGSDDEGAGGGGK VAFEVTDLDVFRQLVLNRLT
Sbjct:  964 VMMLDCDRCHRWFHGRCVGITSQEDCPEEWFCVDCTILRQVTAQKRAHANRQST---------EFGDE-AGSDDEGAGGGGKTVAFEVTDLDVFRQLVLNRLT 1056
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BLAST of mRNA_Ecto-sp13_S_contig19069.6069.1 vs. uniprot
Match: A0A7S3VWS1_EMIHU (Hypothetical protein (Fragment) n=1 Tax=Emiliania huxleyi TaxID=2903 RepID=A0A7S3VWS1_EMIHU)

HSP 1 Score: 71.2 bits (173), Expect = 2.910e-12
Identity = 30/53 (56.60%), Postives = 37/53 (69.81%), Query Frame = 3 
Query:    9 MLDCDRCHRWFHGHCVGITSQED-CPEEWFCVDCTILRQVSAQKHAHAKRQSM 164
            MLDCDRCHRWFHG CVGI +QED  P EWFC  C + R V+ Q+   ++  S+
Sbjct:  168 MLDCDRCHRWFHGSCVGIEAQEDDLPSEWFCESCQLSRAVTKQRTTISRLLSL 220
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BLAST of mRNA_Ecto-sp13_S_contig19069.6069.1 vs. uniprot
Match: A0A7S4RR27_9STRA (Sister chromatid cohesion protein n=6 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A7S4RR27_9STRA)

HSP 1 Score: 69.7 bits (169), Expect = 1.070e-11
Identity = 44/115 (38.26%), Postives = 56/115 (48.70%), Query Frame = 3 
Query:    9 MLDCDRCHRWFHGHCVGITSQEDCPEEWFCVDCTILRQVSAQKHA-------HAKRQ-------SMDAASHDLTKEFGDEAAGSDDEGAGGGGKVVAFEVTDLDVFRQLVLNRLT 311
            MLDCDRCH WFHG CVG+T ++  P+ W C +CT+   V  Q  A       H KR+       S     H   K+  D A  +D  G          EV D  +FRQL+LN +T
Sbjct:  507 MLDCDRCHAWFHGSCVGVT-KDSLPDVWICDECTLQVMVLEQTQAFVERCSNHQKRRVKGTEEDSQKQKRHKKAKQKKDIAQANDVAGDQTEASDNVPEVDDTHIFRQLLLNYMT 620
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BLAST of mRNA_Ecto-sp13_S_contig19069.6069.1 vs. uniprot
Match: A0A7S1CNW5_9STRA (Hypothetical protein (Fragment) n=1 Tax=Bicosoecida sp. CB-2014 TaxID=1486930 RepID=A0A7S1CNW5_9STRA)

HSP 1 Score: 68.2 bits (165), Expect = 1.270e-11
Identity = 31/56 (55.36%), Postives = 36/56 (64.29%), Query Frame = 3 
Query:    9 MLDCDRCHRWFHGHCVGITSQEDCPEEWFCVDCTILRQVSA-QKHAHAKRQSMDAA 173
            MLDCD CHRWFHG CVGI  +E  PE W+C DC +L QV   Q H    R++  AA
Sbjct:   55 MLDCDTCHRWFHGDCVGIR-EEYAPEAWYCDDCVLLHQVKVVQAHIETVRKTAAAA 109
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BLAST of mRNA_Ecto-sp13_S_contig19069.6069.1 vs. uniprot
Match: A0A7S4B8G5_CHRCT (Sister chromatid cohesion protein (Fragment) n=1 Tax=Chrysotila carterae TaxID=13221 RepID=A0A7S4B8G5_CHRCT)

HSP 1 Score: 68.6 bits (166), Expect = 2.730e-11
Identity = 36/102 (35.29%), Postives = 54/102 (52.94%), Query Frame = 3 
Query:    9 MLDCDRCHRWFHGHCVGI--TSQEDCPEEWFCVDCTILRQVSAQKHAHAKRQSMDAASHDLTKEFGDEAAGSDDEGAGGGGKVVAFEVTDLDVFRQLVLNRL 308
            MLDCD+CHRWFHG CVGI  TS+   PEEWFC  C +   V+ Q+    +  ++        +  GD   G D+       ++     ++ +V +QL++N L
Sbjct:  722 MLDCDKCHRWFHGACVGIYPTSEHSVPEEWFCDSCQLSTAVAEQRQRITRLLAL--------RGDGDSNGGDDENSK----EIYEMLTSETEVTKQLLINHL 811
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BLAST of mRNA_Ecto-sp13_S_contig19069.6069.1 vs. uniprot
Match: B8BTF2_THAPS (Sister chromatid cohesion protein n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B8BTF2_THAPS)

HSP 1 Score: 65.9 bits (159), Expect = 2.420e-10
Identity = 29/62 (46.77%), Postives = 40/62 (64.52%), Query Frame = 3 
Query:    9 MLDCDRCHRWFHGHCVGITSQEDCPEEWFCVDCTILRQVSAQKH--AHAKRQSMDAASHDLT 188
            M+DCDRCH WFHG C+GIT ++  P+EWFC +CT+   +  Q +  A+  R+S    S D T
Sbjct:  864 MVDCDRCHSWFHGSCIGIT-KDTVPDEWFCDECTMQTTIMKQANVFANTNRESNKLTSKDHT 924
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BLAST of mRNA_Ecto-sp13_S_contig19069.6069.1 vs. uniprot
Match: A0A7S2UG10_9STRA (Sister chromatid cohesion protein (Fragment) n=1 Tax=Attheya septentrionalis TaxID=420275 RepID=A0A7S2UG10_9STRA)

HSP 1 Score: 64.7 bits (156), Expect = 6.120e-10
Identity = 39/101 (38.61%), Postives = 51/101 (50.50%), Query Frame = 3 
Query:    9 MLDCDRCHRWFHGHCVGITSQEDCPEEWFCVDCTILRQVSAQKHAHAKRQSMDAASHDLTKEFGDEAAGSDDEGAGGGGKVVAFEVTDLDVFRQLVLNRLT 311
            MLDCDRCH WFHG C GI  ++  PE W C +CT+   V  Q  A A R S    S    K+ G  ++  +             +V +  +FRQL+LN LT
Sbjct:  585 MLDCDRCHNWFHGSCAGIC-KDSLPEVWICNECTMQIMVLEQTKAFATRCSHRRRS---AKKSGKSSSAKN---------CPTPQVDETHIFRQLLLNHLT 672
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BLAST of mRNA_Ecto-sp13_S_contig19069.6069.1 vs. uniprot
Match: A0A7S3QDB7_9STRA (Sister chromatid cohesion protein (Fragment) n=1 Tax=Chaetoceros debilis TaxID=122233 RepID=A0A7S3QDB7_9STRA)

HSP 1 Score: 62.0 bits (149), Expect = 5.390e-9
Identity = 31/70 (44.29%), Postives = 43/70 (61.43%), Query Frame = 3 
Query:    9 MLDCDRCHRWFHGHCVGITSQEDCPEEWFCVDCTILRQVSAQ------KHAHAKRQSMDAA--SHDLTKE 194
            MLDCDRCH WFHG C+G  ++++ PE+W C +CT+   V  Q      K+   +R + D A  S +LTKE
Sbjct:  204 MLDCDRCHNWFHGSCIGF-AKDNLPEKWICDECTMQLLVHDQMKIFSTKYGQRQRNTKDDAPNSLELTKE 272
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BLAST of mRNA_Ecto-sp13_S_contig19069.6069.1 vs. uniprot
Match: K0SHJ0_THAOC (Sister chromatid cohesion protein n=1 Tax=Thalassiosira oceanica TaxID=159749 RepID=K0SHJ0_THAOC)

HSP 1 Score: 61.6 bits (148), Expect = 7.430e-9
Identity = 30/54 (55.56%), Postives = 34/54 (62.96%), Query Frame = 3 
Query:    9 MLDCDRCHRWFHGHCVGITSQEDCPEEWFCVDCTILRQVSAQKHAHAKRQSMDA 170
            MLDCDRCH WFHG CVGIT ++  PE W C DCT+  Q S    A A  Q  D+
Sbjct:  720 MLDCDRCHSWFHGSCVGIT-KDAVPETWVCDDCTL--QGSMLDQAKAFTQGKDS 770
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig19069.6069.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 22
Match NameE-valueIdentityDescription
D7FSV8_ECTSI1.650e-5895.15Sister chromatid cohesion protein n=1 Tax=Ectocarp... [more]
A0A6H5JLU6_9PHAE2.810e-4884.47PHD-type domain-containing protein n=1 Tax=Ectocar... [more]
A0A7S3VWS1_EMIHU2.910e-1256.60Hypothetical protein (Fragment) n=1 Tax=Emiliania ... [more]
A0A7S4RR27_9STRA1.070e-1138.26Sister chromatid cohesion protein n=6 Tax=Ditylum ... [more]
A0A7S1CNW5_9STRA1.270e-1155.36Hypothetical protein (Fragment) n=1 Tax=Bicosoecid... [more]
A0A7S4B8G5_CHRCT2.730e-1135.29Sister chromatid cohesion protein (Fragment) n=1 T... [more]
B8BTF2_THAPS2.420e-1046.77Sister chromatid cohesion protein n=1 Tax=Thalassi... [more]
A0A7S2UG10_9STRA6.120e-1038.61Sister chromatid cohesion protein (Fragment) n=1 T... [more]
A0A7S3QDB7_9STRA5.390e-944.29Sister chromatid cohesion protein (Fragment) n=1 T... [more]
K0SHJ0_THAOC7.430e-955.56Sister chromatid cohesion protein n=1 Tax=Thalassi... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig19069contigEcto-sp13_S_contig19069:127..3224 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start1
Seed ortholog score202.2
Seed ortholog evalue1.1e-49
Seed eggNOG ortholog2880.D7FSV8
Model size313
KEGG koko:K06672
KEGG Pathwayko04111,map04111
Hectar predicted targeting categoryother localisation
Exons4
EggNOG free text desc.cohesin loading
EggNOG OGsKOG1020@1,KOG1020@2759
Cds size306
COG Functional cat.K
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko03036
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462972.3022945-UTR-Ecto-sp13_S_contig19069:126..1311681462972.3022945-UTR-Ecto-sp13_S_contig19069:126..131Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig19069 127..131 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462972.312759-CDS-Ecto-sp13_S_contig19069:131..2001681462972.312759-CDS-Ecto-sp13_S_contig19069:131..200Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig19069 132..200 +
1681462972.3220599-CDS-Ecto-sp13_S_contig19069:1406..14861681462972.3220599-CDS-Ecto-sp13_S_contig19069:1406..1486Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig19069 1407..1486 +
1681462972.3312714-CDS-Ecto-sp13_S_contig19069:2022..21311681462972.3312714-CDS-Ecto-sp13_S_contig19069:2022..2131Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig19069 2023..2131 +
1681462972.343388-CDS-Ecto-sp13_S_contig19069:3176..32241681462972.343388-CDS-Ecto-sp13_S_contig19069:3176..3224Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig19069 3177..3224 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig19069.6069.1prot_Ecto-sp13_S_contig19069.6069.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig19069 132..3224 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig19069.6069.1

>prot_Ecto-sp13_S_contig19069.6069.1 ID=prot_Ecto-sp13_S_contig19069.6069.1|Name=mRNA_Ecto-sp13_S_contig19069.6069.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=102bp
MMLDCDRCHRWFHGHCVGITSQEDCPEEWFCVDCTILRQVSAQKHAHAKR
QSMDAASHDLTKEFGDEAAGSDDEGAGGGGKVVAFEVTDLDVFRQLVLNR
LT
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mRNA from alignment at Ecto-sp13_S_contig19069:127..3224+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig19069.6069.1 ID=mRNA_Ecto-sp13_S_contig19069.6069.1|Name=mRNA_Ecto-sp13_S_contig19069.6069.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=3098bp|location=Sequence derived from alignment at Ecto-sp13_S_contig19069:127..3224+ (Ectocarpus species13 EcNAP12_S_4_19m)
GGGTGATGATGCTCGACTGCGACCGGTGTCACCGCTGGTTCCACGGGCAC
TGCGTGGGCATCACCTCTCAGGAGGTGAGTAGAAAAAAATCTCTCGCGCT
TTCTTTCGTTTCCTTTGGTACAAAAGCTGGACCGTTTTCTGTCTCGTCTT
TTTTTTTTTTTCCTCCAGCACCCCAGCTTTTGTCTTCCCCTTACGGCATC
AAATGCCTACCGAACAGAGTCTCGCCGTTTCTGCCGAGGAACCTCAGGCC
CGCGGTCACGCACGTGCTCTAGTGGTTGTTTTGTCGATTGTCCTTTTTTC
TACTTCTGTTGCTTGTGACGTGTTTGTTAGCCCCTGATATTCTATAATTC
GTTTTGTGGCTCTTACCTGCGGCCGTTTTCGAACTCTTGTGTCATGTCGA
TGCGTTGCGTGTTTCGCGTTTGTGACGCTCGTTTTTTTTGTTTTGTTGTT
TGTCGCTGCTACATGCTCTGCGGCGCATCGTGTTGTGCTCGGTTTTGGTT
TTGGTGTTGGTTTTGTGGTTAGTCTCTGCTTCATGGTTTTGTGATGGTTG
TTGGCTCCCTGCTCCTATATGCATACCTGATTTCGTTGTGGCACCTCAGC
TTCATAGACATCACGTTTGTTTGCCTTGCGCAGCTTTTTCCAAGTTGCGT
GTGAAGGGGGTTGGGTCTCCGCTGCAACTTTATCGTGCGTTTTTCTTCAT
GCAGATCATTTCGTTTTGTTTTTCACACATCGTCTCCGTCGGTTCTGTCT
GTTTCTTTATTCCTGTGTGTGTGCTCCGCGCCTTTTCTTTTTTTTGCTTG
GGGCTCCTCCTTCGTAAGTTTTCGTTCTTGGTACGTGTGCCAACATCTTT
TGTGTGCGGCGCATTTTTTTTTCTGCTTGAGGCCCTTCCCTCGTATGCAT
ATGTTTCTGAGCTTCGTTTCTGTGCCAACATGATTGGTGTTGTCGTCTGC
CTGTGCTATGGCTGCCTGGCACTGGTACTGCTGTTTGGCACTGATGTGCT
GTTCGACACTGTTTGGTACTGGTCGATACTGAATATTCTGTCTGACAATT
GTATTGACACTGATTTGAACTGTTTTGATACTGTTTGGCACTGTTTGACA
CTGATCGACAATGAAATGGCTGTCTGACATTGTTTTGACACTGTTTTGAC
ATTGGTACTGCTGTCTGACACTGAGACTGCTGTTTTCAACTGATTCGCAC
TGTTTGGCACTGATACTGCCATCTACAATTGATACGGCTGTCTGACATCG
ATTGATTGGTACTTTTTGTCACTTTGCCAGGACTGTCCCGAGGAGTGGTT
TTGCGTGGACTGCACCATTCTCCGACAGGTGTCCGCTCAGAAGCACGCCC
ACGCCAAAAGGTGTGGTTTGTGTCGCTGGCCGTTGCCGACGTCGTGCTAC
GAGTCGCCGCCGTTGTTGTTGTTGTTATTGCCGTTGTCATGCTAGCGTCG
TCTTCGTTATTGTTGACGCGTTTTGTTGCTGCTGGCGCTGCTAGTGTTTT
GTTTTGTAACATGACTCACCCACCACCTATGAAGCAAGTCAAAACTCTGA
ACATTTTTCGGTTTTTATCGGGTGTGTTTGGGGCGCCAGCTCGTCAAGAC
CCCTTCTTGTGTACGTGTGGTGTTATGACTACGAAGATGTTGTTTTTGTT
GCTGTTGCTGTTGCTGTTGCTGTTGCTGCTGCTGCCTGTGTATGTGTGAA
TTGTGCTGTTGTTTCTGCTTCCGGTGCTACCTGTCTTGGCGAAAGTGGTG
CCTTCAGAGGCACACAGAGTGCAGGGGCGCGTCAACAGGAAGAAAGCAAC
CGATGCCACTTGGTAGCCCAGGCTCAAAAGTCTTCGACCAGACGCTCGCA
GAATTTTGGCACCAAACCCTCCCCCCTGTCGATTATCTCGCAACAGGCAA
AGCATGGATGCCGCTTCGCACGACCTGACGAAGGAGTTCGGCGATGAGGC
CGCCGGAAGCGACGATGAGGGTGCGGGAGGCGGCGGCAAAGTCGTCGCTT
TTGAGGTGAGGCTGCTGGCTTCGTGTTTGTCGGGGGGTGGGGGATGAACT
GCACTGAGCATTGATAAATAAATGTTTTGCCGAGGTGTGCAACTGTCCGA
GTGATCGTCCCCTCGGCGGGACTCTCAAAGCTGCGAGTTTCCAAAGCTAC
TCGTGGGAAGGAAGGTGCCATTGGTGCCACCATGGTGGTTTCGAGTGATC
TCGTCCAGAGCTCTATGCGGGGGTACTGCATCGTTCGGTGTTCTTGCACC
CTCTTGGTAGTGGAGCTCTTGTTTCGATCTCGGAAAGTGGGTCTGAGGGG
ATTGGCCGCACACACTTGTCCTTTTGGTGTTTGCGTATTTTTTGTTGGGG
TGGAACTGCTTGTTTGCGCCCGGGCCCAGAGTTGTGTGTGTTGATTCAGC
AGCCGTAACGAACACAGCAGTAACACGACGGCACGAACACCAACCTCAAC
CACAACAGCGTCAACCAACCACGAGACTCGTCACCGCAGTCGTAGTCAAG
ACCATTGAACGGGGGAGGCCAGGTTAGAATCAAACGAAATTCAGGGAAAT
ACATCATTGAAGCAGAGATGTGTCCCGCCTGGCAAAACTGATCTCTCAAT
AGTGTATAAGGGCTTAAAAACGACTCATACAAGTCCAGCATCCAGCAGAG
TTTCCGCAAGATTTCACTCTGTAAAGGCTTCCACACATGCATTCATTGTT
GGGCCGTCGTCTCGAACGGGTGCTCCCTCTGTACTCGCATACATTCTCAA
ACTGTCAATAGCGGTGGCTTCATCTACGCCGTAAGCCCGCACTTTCTCTT
CGACCCACGATTCACTCAAGTGGCTACAGTACAAAAGGTCTGGTGGTTGG
TTGATGTCGTGGGGGATGACAGATGACAGTGTTGCTGCTGGCAAGGAAAA
CATGAATACGCTCCCCAACCCCCACTCCATCCCCCCAACTCTTGCACTGT
GCCCCTAGGTGACCGACCTCTACATGGTCTAGCCGCTACGGCATGCAAAA
CGTTAGAGTACCCCTTTCCCTCTCCGACCCTTGTACTGCTGTGCTCCCAG
GTGACGGACCTCGACGTGTTCAGGCAGCTGGTGCTCAATAGACTCACG
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig19069:127..3224+

>mRNA_Ecto-sp13_S_contig19069.6069.1 ID=mRNA_Ecto-sp13_S_contig19069.6069.1|Name=mRNA_Ecto-sp13_S_contig19069.6069.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=306bp|location=Sequence derived from alignment at Ecto-sp13_S_contig19069:127..3224+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGATGCTCGACTGCGACCGGTGTCACCGCTGGTTCCACGGGCACTGCGT
GGGCATCACCTCTCAGGAGGACTGTCCCGAGGAGTGGTTTTGCGTGGACT
GCACCATTCTCCGACAGGTGTCCGCTCAGAAGCACGCCCACGCCAAAAGG
CAAAGCATGGATGCCGCTTCGCACGACCTGACGAAGGAGTTCGGCGATGA
GGCCGCCGGAAGCGACGATGAGGGTGCGGGAGGCGGCGGCAAAGTCGTCG
CTTTTGAGGTGACGGACCTCGACGTGTTCAGGCAGCTGGTGCTCAATAGA
CTCACG
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