mRNA_E-siliculosus-1a_F_contig10615.439.1 (mRNA) Ectocarpus siliculosus Ec863f_EcPH12_90f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-siliculosus-1a_F_contig10615.439.1
Unique NamemRNA_E-siliculosus-1a_F_contig10615.439.1
TypemRNA
OrganismEctocarpus siliculosus Ec863f_EcPH12_90f female (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
Homology
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BLAST of mRNA_E-siliculosus-1a_F_contig10615.439.1 vs. uniprot
Match: A0A6H5JEA6_9PHAE (ERCC4 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JEA6_9PHAE)

HSP 1 Score: 192 bits (487), Expect = 1.020e-54
Identity = 97/97 (100.00%), Postives = 97/97 (100.00%), Query Frame = 1 
Query:    1 SSRRGGRRKAQAPLRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDK 291
            SSRRGGRRKAQAPLRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDK
Sbjct:  984 SSRRGGRRKAQAPLRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDK 1080
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BLAST of mRNA_E-siliculosus-1a_F_contig10615.439.1 vs. uniprot
Match: D7FH45_ECTSI (ERCC4 domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FH45_ECTSI)

HSP 1 Score: 187 bits (476), Expect = 3.030e-53
Identity = 95/97 (97.94%), Postives = 96/97 (98.97%), Query Frame = 1 
Query:    1 SSRRGGRRKAQAPLRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDK 291
            SSR+GGRRKAQA LRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDK
Sbjct:  893 SSRKGGRRKAQASLRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDK 989
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BLAST of mRNA_E-siliculosus-1a_F_contig10615.439.1 vs. uniprot
Match: A0A6A0A349_HAELA (DNA repair endonuclease UVH1 (Fragment) n=1 Tax=Haematococcus lacustris TaxID=44745 RepID=A0A6A0A349_HAELA)

HSP 1 Score: 131 bits (329), Expect = 9.970e-38
Identity = 66/94 (70.21%), Postives = 76/94 (80.85%), Query Frame = 1 
Query:   10 RGGRRKAQAPLRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDK 291
            RGG  KAQ P +VVVD+REF S LP +LHQQGFELVPL I VGDYVL+P +CVERK+L DL  S++SGRLY+Q EAM KHYK PVLLIEF  D+
Sbjct:    9 RGGAAKAQ-PRKVVVDMREFMSSLPAVLHQQGFELVPLTIEVGDYVLSPTMCVERKALPDLVSSLASGRLYSQAEAMCKHYKTPVLLIEFEGDR 101
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BLAST of mRNA_E-siliculosus-1a_F_contig10615.439.1 vs. uniprot
Match: A0A3S3NAZ6_9MAGN (DNA repair endonuclease UVH1 n=1 Tax=Cinnamomum micranthum f. kanehirae TaxID=337451 RepID=A0A3S3NAZ6_9MAGN)

HSP 1 Score: 129 bits (323), Expect = 1.210e-34
Identity = 57/93 (61.29%), Postives = 77/93 (82.80%), Query Frame = 1 
Query:   13 GGRRKAQAPLRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDK 291
            GGR+ A+   +V+VD+REF S LPN+LHQ+G  ++P+ + VGDYVL+P ICVERKS++DLF S +SGRLYNQVE M ++Y++PVLLIEF+ DK
Sbjct:   49 GGRKAAERETQVIVDIREFMSSLPNVLHQKGMRIIPVTLEVGDYVLSPLICVERKSIADLFSSFASGRLYNQVETMARYYRIPVLLIEFSQDK 141
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BLAST of mRNA_E-siliculosus-1a_F_contig10615.439.1 vs. uniprot
Match: UPI001F5D44C3 (DNA repair endonuclease UVH1 n=1 Tax=Lolium rigidum TaxID=89674 RepID=UPI001F5D44C3)

HSP 1 Score: 134 bits (336), Expect = 2.320e-34
Identity = 59/93 (63.44%), Postives = 79/93 (84.95%), Query Frame = 1 
Query:   13 GGRRKAQAPLRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDK 291
            GGR+  +  ++V+VD+REF S LPN+LHQ+G  ++P+ + VGDYVL+P ICVERKS+SDLFQS +SGRLYNQVE M+++YK+PVLLIEF+ DK
Sbjct:  747 GGRKPTEKEMQVIVDMREFMSSLPNVLHQKGIRIIPVTLEVGDYVLSPLICVERKSISDLFQSFASGRLYNQVETMIRYYKIPVLLIEFSQDK 839
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BLAST of mRNA_E-siliculosus-1a_F_contig10615.439.1 vs. uniprot
Match: A0A8J4VB27_9ROSI (ERCC4 domain-containing protein n=1 Tax=Castanea mollissima TaxID=60419 RepID=A0A8J4VB27_9ROSI)

HSP 1 Score: 127 bits (320), Expect = 2.470e-34
Identity = 54/93 (58.06%), Postives = 78/93 (83.87%), Query Frame = 1 
Query:   13 GGRRKAQAPLRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDK 291
            GGR++ +  ++V+VD+REF S LPN+LHQ+G  ++P+ + VGDY+++P ICVERKS+ DLF S +SGRLY+QVE M+++YK+PVLLIEF+ DK
Sbjct:   35 GGRKEIEREMQVIVDMREFMSSLPNILHQRGMHIIPVTLEVGDYIISPLICVERKSIQDLFMSFTSGRLYHQVETMVRYYKIPVLLIEFSQDK 127
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BLAST of mRNA_E-siliculosus-1a_F_contig10615.439.1 vs. uniprot
Match: UPI000B8CC7E5 (DNA repair endonuclease UVH1-like isoform X3 n=1 Tax=Carica papaya TaxID=3649 RepID=UPI000B8CC7E5)

HSP 1 Score: 125 bits (315), Expect = 3.500e-34
Identity = 53/93 (56.99%), Postives = 79/93 (84.95%), Query Frame = 1 
Query:   13 GGRRKAQAPLRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDK 291
            GGR++A+  ++V+VD+REF S LPN+LH +G  ++P+ + VGDY+L+P +CVERKS+ DLF S +SGRLY+QVE+M+++Y++PVLLIEF+ DK
Sbjct:   41 GGRKEAEKEMQVIVDVREFMSSLPNMLHLKGMRIIPVTLEVGDYILSPLLCVERKSIQDLFSSFTSGRLYHQVESMVRYYRIPVLLIEFSQDK 133
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BLAST of mRNA_E-siliculosus-1a_F_contig10615.439.1 vs. uniprot
Match: A0A103XQW0_CYNCS (DNA repair nuclease, XPF-type/Helicase n=3 Tax=Cynara cardunculus var. scolymus TaxID=59895 RepID=A0A103XQW0_CYNCS)

HSP 1 Score: 126 bits (316), Expect = 5.040e-34
Identity = 54/93 (58.06%), Postives = 78/93 (83.87%), Query Frame = 1 
Query:   13 GGRRKAQAPLRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDK 291
            GG+++A   ++V+VD+REF S LPN+LHQ+G  ++P+ + VGDY+L+P ICVERKS+ DLF S +SGRLY+QVE M+++Y++PVLLIEF+ DK
Sbjct:    7 GGQKEADKEMQVIVDMREFMSSLPNVLHQKGMNIIPVTLEVGDYILSPMICVERKSIQDLFSSFASGRLYHQVEMMVRYYRMPVLLIEFSQDK 99
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BLAST of mRNA_E-siliculosus-1a_F_contig10615.439.1 vs. uniprot
Match: A0A5J9WL85_9POAL (ERCC4 domain-containing protein n=1 Tax=Eragrostis curvula TaxID=38414 RepID=A0A5J9WL85_9POAL)

HSP 1 Score: 132 bits (332), Expect = 8.040e-34
Identity = 59/93 (63.44%), Postives = 79/93 (84.95%), Query Frame = 1 
Query:   13 GGRRKAQAPLRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDK 291
            GGR+     ++V+VD+REF S LPN+LHQ+G ++VP+ + VGDYVL+P ICVERKS++DLFQS +SGRLYNQVE M+++YK+PVLLIEF+ DK
Sbjct:  750 GGRKAPDKEMQVIVDMREFMSSLPNVLHQKGIQIVPVTLEVGDYVLSPLICVERKSIADLFQSFASGRLYNQVETMVRYYKIPVLLIEFSQDK 842
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BLAST of mRNA_E-siliculosus-1a_F_contig10615.439.1 vs. uniprot
Match: A0A1Y1I3K2_KLENI (Structure-specific endonuclease ERCC1-XPF n=1 Tax=Klebsormidium nitens TaxID=105231 RepID=A0A1Y1I3K2_KLENI)

HSP 1 Score: 132 bits (332), Expect = 8.100e-34
Identity = 60/97 (61.86%), Postives = 80/97 (82.47%), Query Frame = 1 
Query:    1 SSRRGGRRKAQAPLRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVLTPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDK 291
            S+R+ G R+    ++VVVD+REF S LP++LHQQG ++VP+ + VGDY+L+P++CVERKSL+DLFQS  SGRLYNQ EAM ++YK PVLLIEF+ DK
Sbjct:  962 STRKAGGRQKDRAMQVVVDMREFNSSLPSVLHQQGMKIVPVTLEVGDYILSPEVCVERKSLADLFQSFGSGRLYNQAEAMTRYYKTPVLLIEFDQDK 1058
The following BLAST results are available for this feature:
BLAST of mRNA_E-siliculosus-1a_F_contig10615.439.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5JEA6_9PHAE1.020e-54100.00ERCC4 domain-containing protein n=1 Tax=Ectocarpus... [more]
D7FH45_ECTSI3.030e-5397.94ERCC4 domain-containing protein n=1 Tax=Ectocarpus... [more]
A0A6A0A349_HAELA9.970e-3870.21DNA repair endonuclease UVH1 (Fragment) n=1 Tax=Ha... [more]
A0A3S3NAZ6_9MAGN1.210e-3461.29DNA repair endonuclease UVH1 n=1 Tax=Cinnamomum mi... [more]
UPI001F5D44C32.320e-3463.44DNA repair endonuclease UVH1 n=1 Tax=Lolium rigidu... [more]
A0A8J4VB27_9ROSI2.470e-3458.06ERCC4 domain-containing protein n=1 Tax=Castanea m... [more]
UPI000B8CC7E53.500e-3456.99DNA repair endonuclease UVH1-like isoform X3 n=1 T... [more]
A0A103XQW0_CYNCS5.040e-3458.06DNA repair nuclease, XPF-type/Helicase n=3 Tax=Cyn... [more]
A0A5J9WL85_9POAL8.040e-3463.44ERCC4 domain-containing protein n=1 Tax=Eragrostis... [more]
A0A1Y1I3K2_KLENI8.100e-3461.86Structure-specific endonuclease ERCC1-XPF n=1 Tax=... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-siliculosus-1a_F_contig10615contigE-siliculosus-1a_F_contig10615:636..1737 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef902022-09-19
OGS1.0 of Ectocarpus siliculosus Ec863f_EcPH12_90f female2021-02-24
Properties
Property NameValue
Start0
Stop0
Cds size291
Model size291
Exons3
Hectar predicted targeting categoryother localisation
BRITEko00000,ko00001,ko01000,ko03032,ko03400
Best eggNOG OGNA|NA|NA
Best tax levelEukaryota
COG Functional cat.L
EggNOG OGsCOG1948@1,KOG0442@2759
EggNOG free text desc.resolution of meiotic recombination intermediates
GOsGO:0000003,GO:0000014,GO:0000109,GO:0000110,GO:0000280,GO:0000710,GO:0000712,GO:0000724,GO:0000725,GO:0000726,GO:0000735,GO:0000736,GO:0003006,GO:0003674,GO:0003676,GO:0003677,GO:0003684,GO:0003697,GO:0003824,GO:0004518,GO:0004519,GO:0004520,GO:0004536,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0006139,GO:0006259,GO:0006277,GO:0006281,GO:0006289,GO:0006296,GO:0006298,GO:0006302,GO:0006310,GO:0006312,GO:0006725,GO:0006807,GO:0006950,GO:0006974,GO:0006979,GO:0006996,GO:0007049,GO:0007059,GO:0007127,GO:0007131,GO:0007530,GO:0007531,GO:0007533,GO:0007534,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009314,GO:0009628,GO:0009987,GO:0016043,GO:0016787,GO:0016788,GO:0018130,GO:0019438,GO:0022402,GO:0022413,GO:0022414,GO:0030154,GO:0032502,GO:0032505,GO:0032991,GO:0033554,GO:0033683,GO:0034641,GO:0034645,GO:0034654,GO:0035822,GO:0035825,GO:0036297,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044271,GO:0044422,GO:0044424,GO:0044428,GO:0044446,GO:0044464,GO:0045002,GO:0045132,GO:0045165,GO:0046483,GO:0048285,GO:0048869,GO:0050896,GO:0051304,GO:0051307,GO:0051321,GO:0051716,GO:0061982,GO:0071704,GO:0071840,GO:0071897,GO:0090304,GO:0090305,GO:0097159,GO:0098813,GO:0140013,GO:0140097,GO:1901255,GO:1901360,GO:1901362,GO:1901363,GO:1901576,GO:1903046,GO:1990391,GO:1990599
KEGG Pathwayko03420,ko03460,map03420,map03460
KEGG koko:K10848
Preferred nameRAD1
Seed eggNOG ortholog2880.D7FH45
Seed ortholog evalue2.8e-45
Seed ortholog score187.6
Taxonomic scopeEukaryota
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-siliculosus-1a_F_contig10615.439.1prot_E-siliculosus-1a_F_contig10615.439.1Ectocarpus siliculosus Ec863f_EcPH12_90f femalepolypeptideE-siliculosus-1a_F_contig10615 636..1737 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927562.8765502-CDS-E-siliculosus-1a_F_contig10615:635..6981622927562.8765502-CDS-E-siliculosus-1a_F_contig10615:635..698Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig10615 636..698 -
1696318587.7076178-CDS-E-siliculosus-1a_F_contig10615:635..6981696318587.7076178-CDS-E-siliculosus-1a_F_contig10615:635..698Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig10615 636..698 -
1622927562.8883502-CDS-E-siliculosus-1a_F_contig10615:1342..14711622927562.8883502-CDS-E-siliculosus-1a_F_contig10615:1342..1471Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig10615 1343..1471 -
1696318587.7181063-CDS-E-siliculosus-1a_F_contig10615:1342..14711696318587.7181063-CDS-E-siliculosus-1a_F_contig10615:1342..1471Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig10615 1343..1471 -
1622927562.9000835-CDS-E-siliculosus-1a_F_contig10615:1638..17371622927562.9000835-CDS-E-siliculosus-1a_F_contig10615:1638..1737Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig10615 1639..1737 -
1696318587.7263684-CDS-E-siliculosus-1a_F_contig10615:1638..17371696318587.7263684-CDS-E-siliculosus-1a_F_contig10615:1638..1737Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig10615 1639..1737 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-siliculosus-1a_F_contig10615.439.1

>prot_E-siliculosus-1a_F_contig10615.439.1 ID=prot_E-siliculosus-1a_F_contig10615.439.1|Name=mRNA_E-siliculosus-1a_F_contig10615.439.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=polypeptide|length=97bp
SSRRGGRRKAQAPLRVVVDLREFRSVLPNLLHQQGFELVPLVIAVGDYVL
TPQICVERKSLSDLFQSMSSGRLYNQVEAMLKHYKVPVLLIEFNPDK
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mRNA from alignment at E-siliculosus-1a_F_contig10615:636..1737-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-siliculosus-1a_F_contig10615.439.1 ID=mRNA_E-siliculosus-1a_F_contig10615.439.1|Name=mRNA_E-siliculosus-1a_F_contig10615.439.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=mRNA|length=1102bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig10615:636..1737- (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
AGCAGCAGGAGGGGAGGGCGACGGAAGGCACAAGCGCCTCTCAGGGTGGT
CGTGGACCTTCGAGAGTTCCGCAGCGTTCTTCCTAACCTGCTGCATCAGG
TAGAATCCGACACGGCGGTTTTACTGTTAATTTTGTGTTCCTCTATCTTG
CGTCACACACATGTCTCCCTTGTCTACATCTCCATCTGGTCAATGGTCTA
ACCGCTACAGTACATGAGGCATCCAAAACATGAGTACGCCCCCCCGCGAC
CCCCCCCGTTCTCCAGCAAGGGTTTGAGCTGGTCCCGTTGGTCATAGCGG
TCGGTGATTATGTCCTGACGCCCCAGATCTGCGTCGAGCGAAAGAGCCTG
TCCGACTTGTTCCAGTCCATGTCTTCCGGCAGGCTGTACAACCAGGTTTG
TATTTTTTTTTACGGGGAGTGGTGGCAATTGGCGGAGGAATCACCATGCC
TGCCTGTGGTTGATGTTTTCGTATTGGTAGGATGGCGTAGCCGTTGTCGT
TGCCGTTGACTTTGTGCCGTGGCTTGGGTGCCGTTGTTGTTGATGGTGTT
GTTGTTCCTCTCGCTTAACGCGCATGCATGCGAAAGTGAAGAACCGGCCG
AGAGCGATGGTGATGGTGCCGATTTGATGCCCGTGGATGAGTGGGGGCGG
TCAAGTTTCGTGTTGTACTTGTTGCTGTCAAGGACACCGTCTCTTTTTGT
TTGGTTATTGGAAATGGCGTGTTTTTCTTCGTTGTTCACCTCCTATATAC
TCTTGCTTGTTGCAAAAACCACTTGTGTGACCAAGGCTGGTTCATTCAGA
ACAATACCGGTGAAGGACAGTACCGAAAGGGCAAGGATTGACGTCCAAAA
GAAGGCCTAATATCGCCATTGTTCGTTCGACTGGTTGTTTTGGAGATGTT
AACAAACTAATGTGCAAAAGCAACGCAGTCTCGTTTCGGGTTTGTTTTGT
GGTTTGATTGTGTGTTTTGTTGAGATGATATGTGTCACGAAACGGGTGCC
GTCGATGCCTTCGTCGGACCGCGCGTGTCGTGTCCTCAGGTGGAGGCGAT
GCTGAAGCACTACAAGGTGCCCGTCTTGCTAATCGAGTTCAACCCTGACA
AG
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Coding sequence (CDS) from alignment at E-siliculosus-1a_F_contig10615:636..1737-

>mRNA_E-siliculosus-1a_F_contig10615.439.1 ID=mRNA_E-siliculosus-1a_F_contig10615.439.1|Name=mRNA_E-siliculosus-1a_F_contig10615.439.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=CDS|length=582bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig10615:636..1737- (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
AGCAGCAGGAGGGGAGGGCGACGGAAGGCACAAGCGCCTCTCAGGGTGGT
CGTGGACCTTCGAGAGTTCCGCAGCGTTCTTCCTAACCTGCTGCATCAGA
GCAGCAGGAGGGGAGGGCGACGGAAGGCACAAGCGCCTCTCAGGGTGGTC
GTGGACCTTCGAGAGTTCCGCAGCGTTCTTCCTAACCTGCTGCATCAGCA
AGGGTTTGAGCTGGTCCCGTTGGTCATAGCGGTCGGTGATTATGTCCTGA
CGCCCCAGATCTGCGTCGAGCGAAAGAGCCTGTCCGACTTGTTCCAGTCC
ATGTCTTCCGGCAGGCTGTACAACCAGCAAGGGTTTGAGCTGGTCCCGTT
GGTCATAGCGGTCGGTGATTATGTCCTGACGCCCCAGATCTGCGTCGAGC
GAAAGAGCCTGTCCGACTTGTTCCAGTCCATGTCTTCCGGCAGGCTGTAC
AACCAGGTGGAGGCGATGCTGAAGCACTACAAGGTGCCCGTCTTGCTAAT
CGAGTTCAACCCTGACAAGGTGGAGGCGATGCTGAAGCACTACAAGGTGC
CCGTCTTGCTAATCGAGTTCAACCCTGACAAG
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