mRNA_E-siliculosus-1a_F_contig10288.241.1 (mRNA) Ectocarpus siliculosus Ec863f_EcPH12_90f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-siliculosus-1a_F_contig10288.241.1
Unique NamemRNA_E-siliculosus-1a_F_contig10288.241.1
TypemRNA
OrganismEctocarpus siliculosus Ec863f_EcPH12_90f female (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
Homology
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BLAST of mRNA_E-siliculosus-1a_F_contig10288.241.1 vs. uniprot
Match: D7G723_ECTSI (DNA topoisomerase I n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G723_ECTSI)

HSP 1 Score: 114 bits (286), Expect = 5.140e-31
Identity = 69/103 (66.99%), Postives = 72/103 (69.90%), Query Frame = 1 
Query:    7 GTERSGQEGGVPRHHPGDTRQGVRG-QTGIL*PRSGPGEEARRILDRFH-------TWIFVAPRLRAGRVQGVALATVVERDRAHLKFKSADCWDVVANATAA 291
            GTE SGQEG VPRHHP DTRQGVRG +T    PRSGP EEAR ILD           W FVAP L AGRVQ VA A V+ER+ A LK  SADCWDVVANATAA
Sbjct:   16 GTESSGQEGSVPRHHPEDTRQGVRGTRTDWREPRSGPREEARCILDCLAGFTLTPVIWNFVAPGLSAGRVQSVAPAMVIERECARLKSNSADCWDVVANATAA 118
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BLAST of mRNA_E-siliculosus-1a_F_contig10288.241.1 vs. uniprot
Match: D8LDU2_ECTSI (DNA topoisomerase (Fragment) n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LDU2_ECTSI)

HSP 1 Score: 79.0 bits (193), Expect = 4.770e-15
Identity = 45/65 (69.23%), Postives = 47/65 (72.31%), Query Frame = 1 
Query:  118 EEARRILDRFH-------TWIFVAPRLRAGRVQGVALATVVERDRAHLKFKSADCWDVVANATAA 291
            +EARRILDR          W FVAP L AGRVQ VALA VVER+RA LKFKSAD WDVVAN TAA
Sbjct:  249 QEARRILDRLAGFTLTPVIWNFVAPGLSAGRVQSVALAMVVERERARLKFKSADYWDVVANVTAA 313
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BLAST of mRNA_E-siliculosus-1a_F_contig10288.241.1 vs. uniprot
Match: A0A010YGE9_9ACTN (DNA topoisomerase 1 n=4 Tax=Cryptosporangium TaxID=65502 RepID=A0A010YGE9_9ACTN)

HSP 1 Score: 59.7 bits (143), Expect = 2.760e-8
Identity = 31/58 (53.45%), Postives = 38/58 (65.52%), Query Frame = 1 
Query:  118 EEARRILDRFH-------TWIFVAPRLRAGRVQGVALATVVERDRAHLKFKSADCWDV 270
            +E RRILDR +        W  V PRL AGRVQ VA   VVER+RA +KF+SA+ WD+
Sbjct:  172 QETRRILDRLYGYEVSPVLWKKVMPRLSAGRVQSVATRIVVERERARMKFRSAEYWDI 229
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BLAST of mRNA_E-siliculosus-1a_F_contig10288.241.1 vs. uniprot
Match: A0A543AWD6_9ACTN (DNA topoisomerase 1 n=1 Tax=Stackebrandtia endophytica TaxID=1496996 RepID=A0A543AWD6_9ACTN)

HSP 1 Score: 58.9 bits (141), Expect = 5.150e-8
Identity = 32/61 (52.46%), Postives = 39/61 (63.93%), Query Frame = 1 
Query:  118 EEARRILDRFH-------TWIFVAPRLRAGRVQGVALATVVERDRAHLKFKSADCWDVVAN 279
            +EARRILDR +        W  V PRL AGRVQ VA   VVER+RA +KF +AD W + A+
Sbjct:  152 QEARRILDRLYGYEVSPVLWKKVMPRLSAGRVQSVATRIVVERERARMKFVTADYWGITAH 212
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BLAST of mRNA_E-siliculosus-1a_F_contig10288.241.1 vs. uniprot
Match: A0A6J4HFQ2_9ACTN (DNA topoisomerase 1 n=1 Tax=uncultured Corynebacteriales bacterium TaxID=581187 RepID=A0A6J4HFQ2_9ACTN)

HSP 1 Score: 58.5 bits (140), Expect = 7.040e-8
Identity = 30/58 (51.72%), Postives = 38/58 (65.52%), Query Frame = 1 
Query:  118 EEARRILDRFH-------TWIFVAPRLRAGRVQGVALATVVERDRAHLKFKSADCWDV 270
            +E RRILDR +        W  V P+L AGRVQ VA   VVER+RA +KF+SA+ WD+
Sbjct:  172 QETRRILDRLYGYEVSPVLWKKVMPKLSAGRVQSVATRLVVERERARMKFRSAEYWDI 229
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BLAST of mRNA_E-siliculosus-1a_F_contig10288.241.1 vs. uniprot
Match: A0A835Z3T3_9STRA (DNA topoisomerase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z3T3_9STRA)

HSP 1 Score: 58.5 bits (140), Expect = 7.170e-8
Identity = 34/73 (46.58%), Postives = 45/73 (61.64%), Query Frame = 1 
Query:  118 EEARRILDRF-----------HT-----WIFVAPRLRAGRVQGVALATVVERDRAHLKFKSADCWDVVANATA 288
            +EARRI+D             +T     W  +AP+L AGRVQ VALA +++R+ A LKFKSA+ WD+ AN TA
Sbjct:  499 QEARRIIDSILDRKYPVLLAGYTMSPLLWRKIAPKLSAGRVQSVALAMIIKREMARLKFKSAEYWDLNANVTA 571
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BLAST of mRNA_E-siliculosus-1a_F_contig10288.241.1 vs. uniprot
Match: A0A7W1E0A8_9ACTN (DNA topoisomerase 1 n=1 Tax=Actinomycetia bacterium TaxID=1883427 RepID=A0A7W1E0A8_9ACTN)

HSP 1 Score: 58.2 bits (139), Expect = 9.570e-8
Identity = 29/58 (50.00%), Postives = 39/58 (67.24%), Query Frame = 1 
Query:  118 EEARRILDRFH-------TWIFVAPRLRAGRVQGVALATVVERDRAHLKFKSADCWDV 270
            +EARRILDR +        W  V P+L AGRVQ VA   +VER+RA ++F++AD WD+
Sbjct:  150 QEARRILDRLYGYEVSPVLWKKVMPKLSAGRVQSVATRILVERERARMRFRAADWWDI 207
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BLAST of mRNA_E-siliculosus-1a_F_contig10288.241.1 vs. uniprot
Match: A0A562V2E0_9ACTN (DNA topoisomerase 1 n=1 Tax=Stackebrandtia albiflava TaxID=406432 RepID=A0A562V2E0_9ACTN)

HSP 1 Score: 57.8 bits (138), Expect = 1.310e-7
Identity = 31/61 (50.82%), Postives = 39/61 (63.93%), Query Frame = 1 
Query:  118 EEARRILDRFH-------TWIFVAPRLRAGRVQGVALATVVERDRAHLKFKSADCWDVVAN 279
            +EARRILDR +        W  V PRL AGRVQ VA   VVER+RA ++F +AD W + A+
Sbjct:  152 QEARRILDRLYGYEVSPVLWKKVRPRLSAGRVQSVATRIVVERERARMRFVTADYWGITAH 212
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BLAST of mRNA_E-siliculosus-1a_F_contig10288.241.1 vs. uniprot
Match: D3Q5R3_STANL (DNA topoisomerase 1 n=1 Tax=Stackebrandtia nassauensis (strain DSM 44728 / CIP 108903 / NRRL B-16338 / NBRC 102104 / LLR-40K-21) TaxID=446470 RepID=D3Q5R3_STANL)

HSP 1 Score: 57.4 bits (137), Expect = 1.790e-7
Identity = 31/60 (51.67%), Postives = 38/60 (63.33%), Query Frame = 1 
Query:  118 EEARRILDRFH-------TWIFVAPRLRAGRVQGVALATVVERDRAHLKFKSADCWDVVA 276
            +EARRILDR +        W  V PRL AGRVQ VA   VVER+RA ++F +AD W + A
Sbjct:  152 QEARRILDRLYGYEVSPVLWKKVMPRLSAGRVQSVATRIVVERERARMRFVTADYWGITA 211
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BLAST of mRNA_E-siliculosus-1a_F_contig10288.241.1 vs. uniprot
Match: A0A518F0G3_9BACT (DNA topoisomerase 1 n=1 Tax=Planctomycetes bacterium Poly30 TaxID=2528018 RepID=A0A518F0G3_9BACT)

HSP 1 Score: 57.4 bits (137), Expect = 1.790e-7
Identity = 29/63 (46.03%), Postives = 41/63 (65.08%), Query Frame = 1 
Query:  118 EEARRILDRFH-------TWIFVAPRLRAGRVQGVALATVVERDRAHLKFKSADCWDVVANAT 285
            +E+RRILDR +        W  + PRL AGRVQ VA   +V+R+RA ++F+ A+ WD+VA  T
Sbjct:  149 QESRRILDRLYGYEVSPILWRKIKPRLSAGRVQSVATKILVDRERARMRFREAEYWDLVAQFT 211
The following BLAST results are available for this feature:
BLAST of mRNA_E-siliculosus-1a_F_contig10288.241.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7G723_ECTSI5.140e-3166.99DNA topoisomerase I n=1 Tax=Ectocarpus siliculosus... [more]
D8LDU2_ECTSI4.770e-1569.23DNA topoisomerase (Fragment) n=1 Tax=Ectocarpus si... [more]
A0A010YGE9_9ACTN2.760e-853.45DNA topoisomerase 1 n=4 Tax=Cryptosporangium TaxID... [more]
A0A543AWD6_9ACTN5.150e-852.46DNA topoisomerase 1 n=1 Tax=Stackebrandtia endophy... [more]
A0A6J4HFQ2_9ACTN7.040e-851.72DNA topoisomerase 1 n=1 Tax=uncultured Corynebacte... [more]
A0A835Z3T3_9STRA7.170e-846.58DNA topoisomerase n=1 Tax=Tribonema minus TaxID=30... [more]
A0A7W1E0A8_9ACTN9.570e-850.00DNA topoisomerase 1 n=1 Tax=Actinomycetia bacteriu... [more]
A0A562V2E0_9ACTN1.310e-750.82DNA topoisomerase 1 n=1 Tax=Stackebrandtia albifla... [more]
D3Q5R3_STANL1.790e-751.67DNA topoisomerase 1 n=1 Tax=Stackebrandtia nassaue... [more]
A0A518F0G3_9BACT1.790e-746.03DNA topoisomerase 1 n=1 Tax=Planctomycetes bacteri... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-siliculosus-1a_F_contig10288contigE-siliculosus-1a_F_contig10288:717..2068 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus siliculosus 1a female vs UniRef902022-09-19
OGS1.0 of Ectocarpus siliculosus Ec863f_EcPH12_90f female2021-02-24
Properties
Property NameValue
Start1
Stop0
Cds size288
Model size294
Exons3
Hectar predicted targeting categoryother localisation
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-siliculosus-1a_F_contig10288.241.1prot_E-siliculosus-1a_F_contig10288.241.1Ectocarpus siliculosus Ec863f_EcPH12_90f femalepolypeptideE-siliculosus-1a_F_contig10288 721..2068 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927531.918669-UTR-E-siliculosus-1a_F_contig10288:716..7201622927531.918669-UTR-E-siliculosus-1a_F_contig10288:716..720Ectocarpus siliculosus Ec863f_EcPH12_90f femaleUTRE-siliculosus-1a_F_contig10288 717..720 +
1696318571.29734-UTR-E-siliculosus-1a_F_contig10288:716..7201696318571.29734-UTR-E-siliculosus-1a_F_contig10288:716..720Ectocarpus siliculosus Ec863f_EcPH12_90f femaleUTRE-siliculosus-1a_F_contig10288 717..720 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927531.9346979-CDS-E-siliculosus-1a_F_contig10288:720..8131622927531.9346979-CDS-E-siliculosus-1a_F_contig10288:720..813Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig10288 721..813 +
1696318571.3051636-CDS-E-siliculosus-1a_F_contig10288:720..8131696318571.3051636-CDS-E-siliculosus-1a_F_contig10288:720..813Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig10288 721..813 +
1622927531.952529-CDS-E-siliculosus-1a_F_contig10288:1368..14271622927531.952529-CDS-E-siliculosus-1a_F_contig10288:1368..1427Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig10288 1369..1427 +
1696318571.3128223-CDS-E-siliculosus-1a_F_contig10288:1368..14271696318571.3128223-CDS-E-siliculosus-1a_F_contig10288:1368..1427Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig10288 1369..1427 +
1622927531.9714148-CDS-E-siliculosus-1a_F_contig10288:1932..20681622927531.9714148-CDS-E-siliculosus-1a_F_contig10288:1932..2068Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig10288 1933..2068 +
1696318571.3203878-CDS-E-siliculosus-1a_F_contig10288:1932..20681696318571.3203878-CDS-E-siliculosus-1a_F_contig10288:1932..2068Ectocarpus siliculosus Ec863f_EcPH12_90f femaleCDSE-siliculosus-1a_F_contig10288 1933..2068 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-siliculosus-1a_F_contig10288.241.1

>prot_E-siliculosus-1a_F_contig10288.241.1 ID=prot_E-siliculosus-1a_F_contig10288.241.1|Name=mRNA_E-siliculosus-1a_F_contig10288.241.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=polypeptide|length=96bp
MEPNVPVKRAVFHDITQETLVKAFEVRREFFNLGQAQEKRPAASLTAFIP
GSSSLRGFAPDGCKVWRLRRWSSATARTSSSSPRIAGTWSPTLPPR
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mRNA from alignment at E-siliculosus-1a_F_contig10288:717..2068+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-siliculosus-1a_F_contig10288.241.1 ID=mRNA_E-siliculosus-1a_F_contig10288.241.1|Name=mRNA_E-siliculosus-1a_F_contig10288.241.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=mRNA|length=1352bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig10288:717..2068+ (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
GGTCATGGAACCGAACGTTCCGGTCAAGAGGGCGGTGTTCCACGACATCA
CCCAGGAGACACTCGTCAAGGCGTTCGAGGTCAGACGGGAATTCTTTGTT
ATGTTGGTGGTGGTTTGTTGGAGTGTGTGTTTGTTGTTGTTGTTATCGAT
GGCGTTGATGTCGTTGTCGTTTTGGTGCCTGGAAGATCGTTTTTCGACAA
CTACATGTAGTCGCACGGTCATGAGTCAGACTTTATGTTGGCAACGACGG
GGACAACGGGCCCTGCACCAACGCCGGTGTACTCGACGCAACCGACCGTC
CTGCAACAGCCCTCCACCATGTTGTCCGCAGCCACATGAGCGTCAGGATT
CCATGTTTCCATGTTTAGATCCTCTCTGACGCCCGCGCACCCTTCTCCGA
CGACAGCAACGGCGACAACGACGTAGTTGGTATTCAACCACCAAGCCTTC
ACCGGATCAATCCCCCTTCCACTCCCTCCCATGTACCATGTCGTCATTGG
TGCGAGTTTCGATGATCACGCGACTATTGAGGCAAGAAGCAGATGAGGTA
TCCCCGGGCCCCCCTCTCTTGTCTCTGGCGGTGCCGTGCATTTTCCTCTC
TTTTTTTTGTCTTTTCTTTATCATTCTTCAGGAACCAGGACAGATTGACG
AGAACCTCGGTCAGGCCCAGGAGAAGAGGCCCGCCGCATCCTTGACCGCT
TTCATACCTGGGTAAGAGAGGGAAGGGGGGAAAGGAAGTCATATGGACAA
TGGAGCATTCGCCGGGCTAGTGGGTAGAGGAGTCTGGTACGTTTGCGTCC
GGGCTAGTTGATGGGGAACCTTGGAAGTCTGGTGGTGTTCCATGGATTTC
ATCGACGTTGCAGTTGAGAAAGGGGACTGCTGATTCTCCTCCCGGTGTTG
CCCGTGTTGTTGGTTGTTTTTCGTTCTATGTTGGAAAATAGCTGATACGG
TTCCCATTGGAATCGCGCAAGTGGCCCGCAGTATCCCCCCCCCCCCCCCA
TCTGGTCTGGTCACAGTAAACGCAAGGATGATCACCAGCTTCTGAATCAT
CCTTGTGAAACCTATAGCCAGGGCCGGGCGAGAACCCGGCATGTGGCTCC
GTCCCGGCATGCGAAGTCTATCGTCTAACGAACTACGGCGTGCGTTCACA
CGAGTCCCCCCCCCCCTGCCCGTGCGTGCTGGTTCACACAGCTTCACCCT
GACCCCGGTTATCTAGATCTTCGTCGCTCCGAGGCTTCGCGCCGGACGGG
TGCAAGGTGTGGCGCTTGCGACGGTGGTCGAGCGCGACCGCGCGCACCTC
AAGTTCAAGTCCGCGGATTGCTGGGACGTGGTCGCCAACGCTACCGCCGC
GG
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Coding sequence (CDS) from alignment at E-siliculosus-1a_F_contig10288:717..2068+

>mRNA_E-siliculosus-1a_F_contig10288.241.1 ID=mRNA_E-siliculosus-1a_F_contig10288.241.1|Name=mRNA_E-siliculosus-1a_F_contig10288.241.1|organism=Ectocarpus siliculosus Ec863f_EcPH12_90f female|type=CDS|length=576bp|location=Sequence derived from alignment at E-siliculosus-1a_F_contig10288:717..2068+ (Ectocarpus siliculosus Ec863f_EcPH12_90f female)
ATGGAACCGAACGTTCCGGTCAAGAGGGCGGTGTTCCACGACATCACCCA
GGAGACACTCGTCAAGGCGTTCGAGGTCAGACGGGAATTCTTTATGGAAC
CGAACGTTCCGGTCAAGAGGGCGGTGTTCCACGACATCACCCAGGAGACA
CTCGTCAAGGCGTTCGAGGTCAGACGGGAATTCTTTAACCTCGGTCAGGC
CCAGGAGAAGAGGCCCGCCGCATCCTTGACCGCTTTCATACCTGGAACCT
CGGTCAGGCCCAGGAGAAGAGGCCCGCCGCATCCTTGACCGCTTTCATAC
CTGGATCTTCGTCGCTCCGAGGCTTCGCGCCGGACGGGTGCAAGGTGTGG
CGCTTGCGACGGTGGTCGAGCGCGACCGCGCGCACCTCAAGTTCAAGTCC
GCGGATTGCTGGGACGTGGTCGCCAACGCTACCGCCGCGGATCTTCGTCG
CTCCGAGGCTTCGCGCCGGACGGGTGCAAGGTGTGGCGCTTGCGACGGTG
GTCGAGCGCGACCGCGCGCACCTCAAGTTCAAGTCCGCGGATTGCTGGGA
CGTGGTCGCCAACGCTACCGCCGCGG
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