mRNA_E-fasciculatus_F_contig12053.1602.1 (mRNA) Ectocarpus fasciculatus Ec846f_Ec191_B4_f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_E-fasciculatus_F_contig12053.1602.1
Unique NamemRNA_E-fasciculatus_F_contig12053.1602.1
TypemRNA
OrganismEctocarpus fasciculatus Ec846f_Ec191_B4_f female (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
Homology
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BLAST of mRNA_E-fasciculatus_F_contig12053.1602.1 vs. uniprot
Match: D8LQU2_ECTSI (Major facilitator superfamily permease n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQU2_ECTSI)

HSP 1 Score: 214 bits (545), Expect = 6.580e-64
Identity = 105/106 (99.06%), Postives = 106/106 (100.00%), Query Frame = 1 
Query:    1 FPLLVSVGLCLVNFLMIAFVMPETLPDEKRVKKVDLKQANPLGAVTMATRNQLITGVFACWTLLWIAHVGLQINWINYTDRKFGWGVAQSGASLALMGLLVAVFPK 318
            FPLLVSVGLCLVNF+MIAFVMPETLPDEKRVKKVDLKQANPLGAVTMATRNQLITGVFACWTLLWIAHVGLQINWINYTDRKFGWGVAQSGASLALMGLLVAVFPK
Sbjct:  438 FPLLVSVGLCLVNFVMIAFVMPETLPDEKRVKKVDLKQANPLGAVTMATRNQLITGVFACWTLLWIAHVGLQINWINYTDRKFGWGVAQSGASLALMGLLVAVFPK 543
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BLAST of mRNA_E-fasciculatus_F_contig12053.1602.1 vs. uniprot
Match: A0A6H5J9A0_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5J9A0_9PHAE)

HSP 1 Score: 214 bits (545), Expect = 6.680e-64
Identity = 105/106 (99.06%), Postives = 106/106 (100.00%), Query Frame = 1 
Query:    1 FPLLVSVGLCLVNFLMIAFVMPETLPDEKRVKKVDLKQANPLGAVTMATRNQLITGVFACWTLLWIAHVGLQINWINYTDRKFGWGVAQSGASLALMGLLVAVFPK 318
            FPLLVSVGLCLVNF+MIAFVMPETLPDEKRVKKVDLKQANPLGAVTMATRNQLITGVFACWTLLWIAHVGLQINWINYTDRKFGWGVAQSGASLALMGLLVAVFPK
Sbjct:  439 FPLLVSVGLCLVNFVMIAFVMPETLPDEKRVKKVDLKQANPLGAVTMATRNQLITGVFACWTLLWIAHVGLQINWINYTDRKFGWGVAQSGASLALMGLLVAVFPK 544
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BLAST of mRNA_E-fasciculatus_F_contig12053.1602.1 vs. uniprot
Match: A0A7S2UWW0_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2UWW0_9STRA)

HSP 1 Score: 110 bits (275), Expect = 1.150e-27
Identity = 55/106 (51.89%), Postives = 71/106 (66.98%), Query Frame = 1 
Query:    4 PLLVSVGLCLVNFLMIAFVMPETLP-DEKRVKKVDLKQANPLGAVTMATRNQLITGVFACWTLLWIAHVGLQINWINYTDRKFGWGVAQSGASLALMGLLVAVFPK 318
            PL V +GLC  N    A ++PE    D  R + +DL +ANP+GAV +  R++L+  + A +   W+AH GLQ+NWIN+TD KFGWG A SGASLA MGLLVA  PK
Sbjct:   13 PLYVGMGLCSFNAAFTATLVPEPRARDNGRRRDLDLARANPVGAVKLLARSKLLVALSASYFCTWVAHSGLQVNWINFTDYKFGWGAATSGASLAFMGLLVAAMPK 118
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BLAST of mRNA_E-fasciculatus_F_contig12053.1602.1 vs. uniprot
Match: A0A836CIS1_9STRA (Major facilitator superfamily domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CIS1_9STRA)

HSP 1 Score: 107 bits (268), Expect = 1.430e-25
Identity = 53/106 (50.00%), Postives = 71/106 (66.98%), Query Frame = 1 
Query:    1 FPLLVSVGLCLVNFLMIAFVMPETLPDEKRVKKVDLKQANPLGAVTMATRNQLITGVFACWTLLWIAHVGLQINWINYTDRKFGWGVAQSGASLALMGLLVAVFPK 318
            F + VSV LC +N L+   VMPE+L   +R   VD   ANP+GA+ + +R+ +  G+  C+ L WIA+ GLQIN+ NYT++K  W  AQSG  LALMG+LVAV PK
Sbjct:   99 FSVCVSVALCALNALLTGLVMPESLHRARRKPAVDFTMANPVGALRLLSRSPISLGISICYCLHWIANAGLQINFFNYTEKKLSWTPAQSGGGLALMGILVAVAPK 204
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BLAST of mRNA_E-fasciculatus_F_contig12053.1602.1 vs. uniprot
Match: A0A7S2CZ70_9STRA (Hypothetical protein n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2CZ70_9STRA)

HSP 1 Score: 91.3 bits (225), Expect = 2.710e-19
Identity = 48/107 (44.86%), Postives = 73/107 (68.22%), Query Frame = 1 
Query:    4 PLLVSVGLCLVNFLMIAFVMPETLPDEKRV-KKVDLKQANPLGAVTMAT-RNQLITGVFACWTLLWIAHVGLQINWINYTDRKFGWGVAQSGASLALMGLLVAVFPK 318
            PLL+  GL L+N  +IAFV PE+LP  +R  +K+DL++ANP+GA+ +   RN L+TG    + L+++A + L   + +Y   KFGWG AQSG  L L+GL++A+ P+
Sbjct:  239 PLLIGAGLQLLNAFLIAFVTPESLPASERAGRKLDLRKANPIGALKLLLGRNPLLTGSAVAYFLVYLAKLCLDSQFTDYALYKFGWGPAQSGPLLVLVGLMLAIVPR 345
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BLAST of mRNA_E-fasciculatus_F_contig12053.1602.1 vs. uniprot
Match: A0A7S0TRX5_HEMAN (Hypothetical protein n=1 Tax=Hemiselmis andersenii TaxID=464988 RepID=A0A7S0TRX5_HEMAN)

HSP 1 Score: 85.9 bits (211), Expect = 2.330e-17
Identity = 42/105 (40.00%), Postives = 68/105 (64.76%), Query Frame = 1 
Query:    4 PLLVSVGLCLVNFLMIAFVMPETLPDEKRVKKVDLKQANPLGAVTMATRNQLITGVFACWTLLWIAHVGLQINWINYTDRKFGWGVAQSGASLALMGLLVAVFPK 318
            P + S  +  VN L++ F++PE+ P++ R ++VDL  ANP GA+ M   + L+ GV   + LLW AH GLQ+ W+N+ D + GW  ++SG  L++ G+ V++ PK
Sbjct:  249 PFIASAVIGFVNSLLV-FLIPESNPEDDR-REVDLANANPWGALRMLHEDPLMFGVAVTYFLLWFAHTGLQVTWMNFFDAQHGWPASKSGMVLSVFGIGVSIMPK 351
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BLAST of mRNA_E-fasciculatus_F_contig12053.1602.1 vs. uniprot
Match: A0A7S2G8W7_9STRA (Hypothetical protein n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2G8W7_9STRA)

HSP 1 Score: 82.8 bits (203), Expect = 4.180e-17
Identity = 43/98 (43.88%), Postives = 67/98 (68.37%), Query Frame = 1 
Query:   31 LVNFLMIAFVMPETLPDEKRV-KKVDLKQANPLGAVTMAT-RNQLITGVFACWTLLWIAHVGLQINWINYTDRKFGWGVAQSGASLALMGLLVAVFPK 318
            L+N  +IAFV PE+LP  +R  +K+DL++ANP+GA+ +   RN L+TG    + L+++A + L   + +Y   KFGWG AQSG  L L+GL++A+ P+
Sbjct:    6 LLNAFLIAFVTPESLPASERAGRKLDLRKANPIGALKLLLGRNPLLTGSAVAYFLVYLAKLCLDSQFTDYALYKFGWGPAQSGPLLVLVGLMLAIVPR 103
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BLAST of mRNA_E-fasciculatus_F_contig12053.1602.1 vs. uniprot
Match: A0A7S0VW17_9CRYP (Hypothetical protein (Fragment) n=1 Tax=Hemiselmis tepida TaxID=464990 RepID=A0A7S0VW17_9CRYP)

HSP 1 Score: 81.6 bits (200), Expect = 2.170e-16
Identity = 40/105 (38.10%), Postives = 66/105 (62.86%), Query Frame = 1 
Query:    4 PLLVSVGLCLVNFLMIAFVMPETLPDEKRVKKVDLKQANPLGAVTMATRNQLITGVFACWTLLWIAHVGLQINWINYTDRKFGWGVAQSGASLALMGLLVAVFPK 318
            P + S  +  VN L+++ + PE+ P++ R ++VDL  ANP GA+ M   N L  G+   + LLW AH GLQ+ W+N+ + + GW  ++SG  L++ G+ ++V PK
Sbjct:   56 PFIASACIGAVNALLVSLI-PESNPEDLR-REVDLANANPWGALKMLHANPLTFGLAVSYFLLWFAHTGLQVTWMNFFETQHGWPASKSGIVLSVFGIGISVMPK 158
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BLAST of mRNA_E-fasciculatus_F_contig12053.1602.1 vs. uniprot
Match: A0A1E4NI89_9GAMM (Tetracycline resistance MFS efflux pump n=2 Tax=Xanthomonadales TaxID=135614 RepID=A0A1E4NI89_9GAMM)

HSP 1 Score: 81.3 bits (199), Expect = 8.610e-16
Identity = 41/102 (40.20%), Postives = 61/102 (59.80%), Query Frame = 1 
Query:    4 PLLVSVGLCLVNFLMIAFVMPETLPDEKRVKKVDLKQANPLGAVTMATRNQLITGVFACWTLLWIAHVGLQINWINYTDRKFGWGVAQSGASLALMGLLVAV 309
            P  V+ GLCLVNF    FV+PE+LP E+R  K D + ANPLG++ +  R   +  +   + LL +A   +   ++ YTD ++GWG    G +LAL+G+L  V
Sbjct:  173 PFWVAAGLCLVNFCYGWFVLPESLPKERRAPKFDWRHANPLGSLLLLKRYPQVLALAMVFFLLALAQFSINSTYVLYTDFRYGWGPQTVGYTLALVGVLTGV 274
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BLAST of mRNA_E-fasciculatus_F_contig12053.1602.1 vs. uniprot
Match: I4VIW2_9GAMM (Major facilitator superfamily protein n=4 Tax=Rhodanobacter TaxID=75309 RepID=I4VIW2_9GAMM)

HSP 1 Score: 79.7 bits (195), Expect = 2.980e-15
Identity = 42/98 (42.86%), Postives = 60/98 (61.22%), Query Frame = 1 
Query:    4 PLLVSVGLCLVNFLMIAFVMPETLPDEKRVKKVDLKQANPLGAVTMATRNQLITGVFACWTLLWIAHVGLQINWINYTDRKFGWGVAQSGASLALMGL 297
            P  V+ GL LVNF    FV+PE+LP E+R K+ + + ANPLGAV +  R   + G+ A + L+ +A   L   ++ YTD +FGWG    G +L L+GL
Sbjct:  138 PFWVAAGLSLVNFCYGWFVLPESLPPERRSKRFEWRHANPLGAVMLLRRYPQVFGLAAVFFLINLAQFSLNSTYVLYTDYRFGWGPQVVGYTLGLVGL 235
The following BLAST results are available for this feature:
BLAST of mRNA_E-fasciculatus_F_contig12053.1602.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LQU2_ECTSI6.580e-6499.06Major facilitator superfamily permease n=1 Tax=Ect... [more]
A0A6H5J9A0_9PHAE6.680e-6499.06Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A7S2UWW0_9STRA1.150e-2751.89Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
A0A836CIS1_9STRA1.430e-2550.00Major facilitator superfamily domain-containing pr... [more]
A0A7S2CZ70_9STRA2.710e-1944.86Hypothetical protein n=1 Tax=Florenciella parvula ... [more]
A0A7S0TRX5_HEMAN2.330e-1740.00Hypothetical protein n=1 Tax=Hemiselmis andersenii... [more]
A0A7S2G8W7_9STRA4.180e-1743.88Hypothetical protein n=1 Tax=Florenciella parvula ... [more]
A0A7S0VW17_9CRYP2.170e-1638.10Hypothetical protein (Fragment) n=1 Tax=Hemiselmis... [more]
A0A1E4NI89_9GAMM8.610e-1640.20Tetracycline resistance MFS efflux pump n=2 Tax=Xa... [more]
I4VIW2_9GAMM2.980e-1542.86Major facilitator superfamily protein n=4 Tax=Rhod... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
E-fasciculatus_F_contig12053contigE-fasciculatus_F_contig12053:291..2114 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female vs UniRef902022-09-19
OGS1.0 of Ectocarpus fasciculatus Ec846f_Ec191_B4_f female2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score77.4
Seed ortholog evalue4.5e-12
Seed eggNOG ortholog1227739.Hsw_2493
Preferred nametetA
KEGG koko:K08151,ko:K08153
KEGG TC2.A.1.2.38,2.A.1.2.39,2.A.1.2.4,2.A.1.2.41,2.A.1.2.68,2.A.1.2.75,2.A.1.2.8
KEGG ModuleM00668,M00717
EggNOG free text desc.Major facilitator Superfamily
EggNOG OGs47N08@768503,4NFM7@976,COG0477@1,COG2814@2
COG Functional cat.EGP
Best tax levelCytophagia
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00002,ko01504,ko02000
Hectar predicted targeting categorysignal peptide
Exons3
Model size321
Cds size321
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_E-fasciculatus_F_contig12053.1602.1prot_E-fasciculatus_F_contig12053.1602.1Ectocarpus fasciculatus Ec846f_Ec191_B4_f femalepolypeptideE-fasciculatus_F_contig12053 291..2114 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927714.1086764-CDS-E-fasciculatus_F_contig12053:290..3801622927714.1086764-CDS-E-fasciculatus_F_contig12053:290..380Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig12053 291..380 -
1695995662.8101327-CDS-E-fasciculatus_F_contig12053:290..3801695995662.8101327-CDS-E-fasciculatus_F_contig12053:290..380Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig12053 291..380 -
1622927714.125033-CDS-E-fasciculatus_F_contig12053:982..11141622927714.125033-CDS-E-fasciculatus_F_contig12053:982..1114Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig12053 983..1114 -
1695995662.824224-CDS-E-fasciculatus_F_contig12053:982..11141695995662.824224-CDS-E-fasciculatus_F_contig12053:982..1114Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig12053 983..1114 -
1622927714.1411638-CDS-E-fasciculatus_F_contig12053:2015..21141622927714.1411638-CDS-E-fasciculatus_F_contig12053:2015..2114Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig12053 2016..2114 -
1695995662.8363109-CDS-E-fasciculatus_F_contig12053:2015..21141695995662.8363109-CDS-E-fasciculatus_F_contig12053:2015..2114Ectocarpus fasciculatus Ec846f_Ec191_B4_f femaleCDSE-fasciculatus_F_contig12053 2016..2114 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_E-fasciculatus_F_contig12053.1602.1

>prot_E-fasciculatus_F_contig12053.1602.1 ID=prot_E-fasciculatus_F_contig12053.1602.1|Name=mRNA_E-fasciculatus_F_contig12053.1602.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=polypeptide|length=107bp
FPLLVSVGLCLVNFLMIAFVMPETLPDEKRVKKVDLKQANPLGAVTMATR
NQLITGVFACWTLLWIAHVGLQINWINYTDRKFGWGVAQSGASLALMGLL
VAVFPKV
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mRNA from alignment at E-fasciculatus_F_contig12053:291..2114-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_E-fasciculatus_F_contig12053.1602.1 ID=mRNA_E-fasciculatus_F_contig12053.1602.1|Name=mRNA_E-fasciculatus_F_contig12053.1602.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=mRNA|length=1824bp|location=Sequence derived from alignment at E-fasciculatus_F_contig12053:291..2114- (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
TTCCCCCTCCTCGTGAGCGTGGGTCTCTGCCTGGTGAACTTCCTGATGAT
CGCGTTCGTCATGCCGGAGACGTTGCCTGACGAGAAGCGAGTGAAGAAGG
TGAGGTGTTGTGTGGAAAGGGGGGTGTACTCAAGGGTCGTGTAGGCCGTA
GTCGTTAGGTCATTGACTTCGCATTCCCTACGAAGCGGGGACGGAGCACG
CCGGGTTCTCTCACCGGACCAGGCCCTCACATACCAAGCGCGAAACGCCG
TGAGGTATTCGGCGAATCGCATGGAGGGTGCTCTGCATCCTGCTAGGAGG
GTGTTCGGCATTATGTGCCTACTTACTTGCATGCGCATGGCAGCTGCGAA
CGACTTAATTTATTTTCTGGCGCTTTCCCCTTCCAGACACAGTCATAAGC
ACTACTGTGTCCTGCCTCGAAGGTGCTTGACCATTTTATCGCAACGTGTT
GCGGTCACCCCCTTTGCGCGGAGTGAGCGTTCTCGACCCCAAACAGTAGA
ATCTACTCGACCACCACAATGGTCACCGCCAACAAACAAAACCTTGTTGT
GTGGAAAGGGGCCTTTATATTCCCGGGTCCCACCCAACTCCCCAGAGGGT
CTTCGGAGGACGCACGCTGATGCTCTTTTGGCGTCGGGTCTCAGGGGCAT
TTTCCTTCGTAGCTGACAGGAACAAGACAGTAATTCAGGACTAAGTGCCA
CCGAACGGTGCAGAGGACAAGCACCTACGTGTCGTCATTTCATGACTCGT
AACTGTCCTTGCACCCCCCAATGGTACAACATGAGAAAAACTGTTGACAT
GCACGTGTGGAAAGCTGCTCTATGTACCGGAAACCCATCAACACCCCGGG
GTAAGGGCTTCGTTGTGGCCGTTACTCGTTTGGTATTCATTTTTTTAATT
ATTATTACTATGTTTCATGTTTAATTTTAGTATGATTTTTGGTTTTGCTG
TAGTCCTTGGCGCTAACGACGACGACGACGACGACGACTGCTTGCAACAG
GTGGACCTGAAGCAGGCGAACCCGCTGGGTGCCGTGACGATGGCCACGCG
CAACCAGCTCATCACGGGCGTGTTCGCTTGCTGGACGCTGCTGTGGATCG
CCCACGTGGGTCTTCAGATCAACTGGATCAACGTGAGAACCTTTTTTTGT
GGGCGTCAGTGCTCCGTGTGTGTATGTGTGTGTGTGTGTGTTGTTTTTTT
CTCGTAATATGACATGATGAACCCATCTCTATCTAAGAAAATAGTCTAAG
ATAGATTAGCAATGAATCTATAGCGCTTTTCGGTCGTTCCTGAGTTCTGG
TTGGCTTGTAGCGACGAGGATTCGTTTTCTTGTTCTGTTTTTGAAACGGT
CTTTTTTCAGAGGTTTGGAACGCAGGGGGGGCACTGGATATGTATATATA
TTTTTGGTGTGACGTGAAGGCACCGAAAGCCACAACCAGCCAGCCGTCTT
GAAAAAGCCCAAAGGGAACGCCGCAACATGGTTGTTGCAAGTGTCATCAT
GCCCTCTGCCTACTACCGCTCGCTCTTTTCCCTGACCTAGTGGTGGTGGT
GGTGGTGCTGCCCCTCTCTCTACTACCTGTCACTCTTTTCCCCGGTCCCA
ATCCGTTTGCTGCTGCTGCGTCTGCTCGTGCCCCTCTCCTTCTACCCCTT
GCTATTTTCCCTGACCCCGATCCTGATGCTGTTTCCGCTGCTGCTGCTGC
TGTTGCTGCTGCTGCCACCCCGGCGATATGGCAGTACACCGACCGGAAGT
TCGGCTGGGGCGTGGCGCAGAGCGGGGCTAGCCTCGCATTGATGGGCCTC
CTCGTCGCCGTCTTCCCGAAGGTA
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Coding sequence (CDS) from alignment at E-fasciculatus_F_contig12053:291..2114-

>mRNA_E-fasciculatus_F_contig12053.1602.1 ID=mRNA_E-fasciculatus_F_contig12053.1602.1|Name=mRNA_E-fasciculatus_F_contig12053.1602.1|organism=Ectocarpus fasciculatus Ec846f_Ec191_B4_f female|type=CDS|length=642bp|location=Sequence derived from alignment at E-fasciculatus_F_contig12053:291..2114- (Ectocarpus fasciculatus Ec846f_Ec191_B4_f female)
TTCCCCCTCCTCGTGAGCGTGGGTCTCTGCCTGGTGAACTTCCTGATGAT
CGCGTTCGTCATGCCGGAGACGTTGCCTGACGAGAAGCGAGTGAAGAAGT
TCCCCCTCCTCGTGAGCGTGGGTCTCTGCCTGGTGAACTTCCTGATGATC
GCGTTCGTCATGCCGGAGACGTTGCCTGACGAGAAGCGAGTGAAGAAGGT
GGACCTGAAGCAGGCGAACCCGCTGGGTGCCGTGACGATGGCCACGCGCA
ACCAGCTCATCACGGGCGTGTTCGCTTGCTGGACGCTGCTGTGGATCGCC
CACGTGGGTCTTCAGATCAACTGGATCAACGTGGACCTGAAGCAGGCGAA
CCCGCTGGGTGCCGTGACGATGGCCACGCGCAACCAGCTCATCACGGGCG
TGTTCGCTTGCTGGACGCTGCTGTGGATCGCCCACGTGGGTCTTCAGATC
AACTGGATCAACTACACCGACCGGAAGTTCGGCTGGGGCGTGGCGCAGAG
CGGGGCTAGCCTCGCATTGATGGGCCTCCTCGTCGCCGTCTTCCCGAAGG
TATACACCGACCGGAAGTTCGGCTGGGGCGTGGCGCAGAGCGGGGCTAGC
CTCGCATTGATGGGCCTCCTCGTCGCCGTCTTCCCGAAGGTA
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