mRNA_A-nodosum_M_contig1124.18.1 (mRNA) Ascophyllum nodosum dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_A-nodosum_M_contig1124.18.1
Unique NamemRNA_A-nodosum_M_contig1124.18.1
TypemRNA
OrganismAscophyllum nodosum dioecious (Ascophyllum nodosum dioecious (Feamainn bhuX, rockweed, Norwegian kelp, knotted kelp, knotted wrack or egg wrack))
Homology
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BLAST of mRNA_A-nodosum_M_contig1124.18.1 vs. uniprot
Match: A0A6H5KZA5_9PHAE (LITAF domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5KZA5_9PHAE)

HSP 1 Score: 159 bits (401), Expect = 5.210e-44
Identity = 84/164 (51.22%), Postives = 106/164 (64.63%), Query Frame = 2 
Query:  239 LPTAEGVFVGDPVAQSLYPNPAP-----VIT--PVSHQYNLNSQASQGRPPSGVSITTGGHHVVTSAAGGMHHTVFLVD--PRG--FYAWGRVPQAFVCNSCGFSGMTSAFTDPCSNAPILAAACCCLVGFWPCSFIPFLIPSLRDTVHQCPGCGLTVGRSQQF 697
            LPTA+G+FVGDPV+QS   + AP     VIT  P S+ +             G+++ +G             HTV +++  P G  F  WGR PQAFVC +CGF+G++   TDPCSN PILAAACCCLVG WPCSF+PFLIP LRDTVH+CP C ++VGRS+QF
Sbjct:   90 LPTAQGIFVGDPVSQSTPYHAAPPAHATVITSAPASYDHVAAXXXXXXXXXXGIAVPSGXXX---------QHTVIVMNAPPPGHQFGEWGRQPQAFVCRACGFTGLSLTMTDPCSNIPILAAACCCLVGCWPCSFVPFLIPELRDTVHRCPNCHVSVGRSRQF 244
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BLAST of mRNA_A-nodosum_M_contig1124.18.1 vs. uniprot
Match: UPI0018DA2B19 (proline-rich protein 2-like n=1 Tax=Patiria miniata TaxID=46514 RepID=UPI0018DA2B19)

HSP 1 Score: 56.2 bits (134), Expect = 8.560e-6
Identity = 28/71 (39.44%), Postives = 37/71 (52.11%), Query Frame = 2 
Query:  482 VPQAFVCNSCGFSGMTSAFTDPCSNAPILAAACCCLVG---FWPCSFIPFLIPSLRDTVHQCPGCGLTVGR 685
            +PQ   C +C  +  T       +   +L    C +     FWP +FIPF IPSL+DTVH CP C  T+GR
Sbjct:  137 LPQIMQCTNCSKTVTTQTSKHVGAAVWVLFGWICLITVWFCFWPLAFIPFCIPSLKDTVHTCPECKFTLGR 207
The following BLAST results are available for this feature:
BLAST of mRNA_A-nodosum_M_contig1124.18.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 2
Match NameE-valueIdentityDescription
A0A6H5KZA5_9PHAE5.210e-4451.22LITAF domain-containing protein n=2 Tax=Ectocarpus... [more]
UPI0018DA2B198.560e-639.44proline-rich protein 2-like n=1 Tax=Patiria miniat... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
A-nodosum_M_contig1124contigA-nodosum_M_contig1124:162950..169854 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ascophyllum nodosum dioecious OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog45351.EDO42963
PFAMszf-LITAF-like
Model size851
Max annot lvl2759|Eukaryota
Hectar predicted targeting categoryother localisation
Exons3
Evalue3.27e-07
EggNOG OGs2CHAU@1|root,2S3NX@2759|Eukaryota
Ec32 ortholog descriptionLPS-induced tumour necrosis factor alpha factor
Ec32 orthologEc-18_003300.1
DescriptionPossible membrane-associated motif in LPS-induced tumor necrosis factor alpha factor (LITAF), also known as PIG7, and other animal proteins.
Cds size696
COG categoryS
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680866523.959076-UTR-A-nodosum_M_contig1124:162949..1630971680866523.959076-UTR-A-nodosum_M_contig1124:162949..163097Ascophyllum nodosum dioeciousUTRA-nodosum_M_contig1124 162950..163097 -
1680866523.998687-UTR-A-nodosum_M_contig1124:169847..1698541680866523.998687-UTR-A-nodosum_M_contig1124:169847..169854Ascophyllum nodosum dioeciousUTRA-nodosum_M_contig1124 169848..169854 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680866523.9700806-CDS-A-nodosum_M_contig1124:163097..1632591680866523.9700806-CDS-A-nodosum_M_contig1124:163097..163259Ascophyllum nodosum dioeciousCDSA-nodosum_M_contig1124 163098..163259 -
1680866523.978132-CDS-A-nodosum_M_contig1124:163647..1640181680866523.978132-CDS-A-nodosum_M_contig1124:163647..164018Ascophyllum nodosum dioeciousCDSA-nodosum_M_contig1124 163648..164018 -
1680866523.9885817-CDS-A-nodosum_M_contig1124:169684..1698471680866523.9885817-CDS-A-nodosum_M_contig1124:169684..169847Ascophyllum nodosum dioeciousCDSA-nodosum_M_contig1124 169685..169847 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_A-nodosum_M_contig1124.18.1prot_A-nodosum_M_contig1124.18.1Ascophyllum nodosum dioeciouspolypeptideA-nodosum_M_contig1124 163098..169847 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_A-nodosum_M_contig1124.18.1

>prot_A-nodosum_M_contig1124.18.1 ID=prot_A-nodosum_M_contig1124.18.1|Name=mRNA_A-nodosum_M_contig1124.18.1|organism=Ascophyllum nodosum dioecious|type=polypeptide|length=232bp
MPRADGYKTLPDDNDAGERTEDRGEAEADGAPTRREGTAAGAAPSSAGAG
STEAATAKDAGSEKQEGARKGDGDLGGLPTAEGVFVGDPVAQSLYPNPAP
VITPVSHQYNLNSQASQGRPPSGVSITTGGHHVVTSAAGGMHHTVFLVDP
RGFYAWGRVPQAFVCNSCGFSGMTSAFTDPCSNAPILAAACCCLVGFWPC
SFIPFLIPSLRDTVHQCPGCGLTVGRSQQFR*
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mRNA from alignment at A-nodosum_M_contig1124:162950..169854-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_A-nodosum_M_contig1124.18.1 ID=mRNA_A-nodosum_M_contig1124.18.1|Name=mRNA_A-nodosum_M_contig1124.18.1|organism=Ascophyllum nodosum dioecious|type=mRNA|length=6905bp|location=Sequence derived from alignment at A-nodosum_M_contig1124:162950..169854- (Ascophyllum nodosum dioecious)
TCGAGAGATGCCTCGCGCAGACGGCTACAAGACCCTTCCCGATGATAACG
ATGCAGGCGAGCGTACGGAAGACCGAGGTGAGGCGGAGGCTGACGGCGCT
CCCACACGGCGAGAGGGCACGGCCGCTGGTGCCGCACCATCGTCGGCTGG
TGCGGGATCAACAGAAGCAGGTTAGCCACGTGCACCACGATTGCTATGGA
CCCGAGGCACTACGGTGGCGGCTACAGTCCGGTCAAATATTCTTTTAACA
GCAGAAGGGCACACTATAAATACTACAGCTTAGACATCTGCTACAACACA
TTTTTCATAAATGCAGAACGCAGTACTCCGTAATTCGTGCGATGATGGGC
CCATATTCAGGTCGTCGACTCAACACGCGGGCTCGTGCACCTGTAACGTA
GGAACTCTATGCCGAAGGCAAAACAAGTAGCTCTTGTACGAGAAGTCAAC
CGCACTCCCCGGGGCAATGACCGTTATTGACTCGTATAGTATCGCAAAAT
ATTTTCGACGGGAAATTTCCATAGAAGGAGCTGGAGACCTTTAGAGCGGA
GAGCGGATCGGTCCTCGCTACGATACCAGTATAAAATTGAGATGTGGTTG
CTTTTGTCAATGCCGGGGATGTGTAGAGAACTACTCTTGATTTGAGTCAT
CGGGAGAAAGTGCGATTACTGTCTTGTAAACAAAAAAGTGCTTGTTTCAG
ATCCAGGCCAAAAGAGAAAACTCCGAGGGCATCCCCACTATCCCGGAGAA
TTCCTATTTGAGAGAAAGAGAGTAACGCACCTTGACTGTTCCAACAGACA
AGACTACTCTACTGCCTCGGAAAGGTGTCCAACGTACCTTGGCTGTTCCA
GCAGACAAGACTACTCTACTGCCTCGGGCAGATGTCCACTACGTGCAGGT
AGTAGGCGTGGGGAAAGAAAGGCGCACAAAAACTGGTCCACAGTGATTCC
TGTAAAGGCAGCACCCGTCTCTGGAACTATCTTGAGGACCACTGGCCCTG
AGCCGGCGGACTATCGGCAGTGAATGCCATTGGCACGCAATTGCGTAACT
TGATTAGCTTGGGACTGACCCGATGGCGGTTGACGGTATAAGATTGGACG
CCGTCGCAAAAAGCGGGAGGAATCCCGCGAGTAAGCACCATATTCAGCCA
GAATGTGGAGAATGAGCAAGCTGACGCGGGACGGGACGGCCGAACCTGTC
TCACGAGACCAAATTCTCTGGCGCGATGGGGGAGAGAAAAAAAACTTTTT
CCCTTTTCAGTTGACCACGAGCAGGATCGGCAAACATACACGGTTGATCC
ATACTCTTCTATAAGGGATCACCGTACATACATGTATGGTCACACATATA
CAACCGGGTAAGGTTGCCAATCCTGCTCGTGGTCAGCTGAACAGGGAAAA
TTAAGCGTTCGCCCCTGAGAATTTGATCTCGCGAGACGGGTTCGGCAGTT
CCGCCCCGCGTCAGCCTGCTCATCTCCATGCTCAGGCTGGATCTGGTGCT
TACCTACGGGATTCCTCCCGAGTTCCGCGGTGGCGTCCATCTATTTATTT
GAAACCGCCATACGCCATCAGGTCAGCCCCGAGTTTATCGGGTCACGCAA
TTGCGTACCGATGGCGTTCACTGCCGACAGTTCACTGCCGACAGTCCGCC
GGCACAGGGCCAGTAACCCTCAAGGTAGCTCCGAACGAGTGCTGCCTTGG
CAGGTCACCATGGACCAATTAATATGCGCCTCTTTTTTACCTGGTACTGA
TCTTCACTGCCCATCCCTCGGGGATTCAACAATGGCTTATGAACAGATAC
TGATGTCCTACTCGTACTAAACTAACTAGACTAATAATACTGCTCAAGCC
GACATCTTGAGAATTTTAGATTTCTATATGGTTCAGAACATCAAAACATA
TATCAGTATAAAGAGTTCTTCCCGAAACTCCTATTAATACGTGAATACGA
TAAAAATAGTTTATTACTCCGGAGTAATATTTATCCCAAGACATTGCACT
AGTTTTTTTGTTTTTTGTTTTCTTTAATCTCACTTATAGCCGATAGACCT
CATCATTACCATCTTGCCTGTGGTCACAAAGGATCTTCCCATCTCTCCCC
GGTCCACGCCTTACAATTTTTTATGGCGATGCAAGTTCAGCACTCTTACA
ACTCGTCAACCAATGGTTGAATTTTACTTACTCACGTTCCCACGCTTTCC
GTTACGAAAGAAAGAACACAAATCCTACTTTGGTAAGAATCGAACTCACG
ACTTCCGCACTAGCAGATGTGCAGGTTACCTACTAGACCACTCGGGCGAC
GATAACAAGACTGTAATTTACTAAATTTTACCCCCACAACATGGTAACCT
GAACTTATAAGCTAGCTCCAAGCGAACACCTTCGCTCAGGCTTGGGTCGA
TAGTAGTGTGTCTCTCGTGCTGGTGTTCCTGCATCGCGATTGGTGCAAAA
TGTCGTTGTCGTCGTTGTTTCGGCACCAACCGTTGTAAGGCTACCAACCC
TGATCAGCTGAACAGGGGAAATAATTTGTGCTCGCGGGACAGGTTCGGCC
GTCCCGTTCCGGGTAAGCCCGGTCACTCTCCGCACCCAGAGGCTGAATCT
GGTCCTTACTCGCGGGTTCCTCCCCAACCCCCCGACGGTGTGCATCTACA
TCTTCAACCGCCCATCAGACCTGTCCCTATCAATAGCTTTATCTGGTCAC
GCAAGAGCGAACCCATGGCGTTCACCGTGGAGAGTGAACCTACGCAGTGC
CAGTATTTGAATAATAATGGGTGTTGGCTATTCAGGTAACCGCGTGAACC
GCTTTATGTGTGTCTCTCTCTTCCCACACCGACATGCCATGGGAATGTGT
GTGTTGCAGGGTACTTGCAGAGCAAGTATAGTGTACTAATAGTGTGCTTC
TCCTCGACAGTCTACTGTTAACAGAAAAAAATCTGAACGCATCGAAACCT
TCCGAGCGCTTACACCCCCGTGCGACGATCCTTGGGTGTAACCCCACTAA
GTCTCATGGAGTTTTGGTTCCAAGCCACCATACCCACCCAAACGTTTCGA
CCGTACAAGTATCCCTCCCGGACGGTGTGGATGGTCGGAAGGTTCAGATT
TTTCTGGTGGAAATGAGATTATAATTGAGATTTTTCTAGAAATGAGATTT
TGTTCTATAATTGAGATTTTTCTATAATTGAGATTTTCTAGAAATGAGAT
TTTGTTCTATAATTGAGATCTTTCTAGAAATGAGATTTTGTTCTATAATT
GAGATTTTTCTATGATGGAGATTTTCTAGAAATGAGATTTTGTTCTATAA
TTGAGATCTTTCTAGAATTGAGATTTAGTTGTACCCCTCCGTGATCCGTC
GCGACCGGGTCAATCAACAGCAGTACTCGTGTAATATAGGGGATAACCCC
ACCGTCATGCCATAGACTTACACCAACTGCCATATGGGGCCAACACGGGA
AGCACGGTACATGCTCAAATGTTTTAGGAACTGGGTAACTTTCGAGCGGA
AACCCCGCGCGGAGAGGCGTTCAATTTTGTTGATTTTTACGGAAATTGAC
TGTACAAGACTTTTAAAAGTAATGCAACAGATTTGCGACCAGAGTGCTGT
AGCCAAATGCCTTCAACACTATATTGGCGGAGTTTGGGACAGCGGATGAA
CAGTACAAAGTGGATGTACAAAGTAGATGCACAAAGTGTGATTCTACGAA
AAGCGACGGGAACCCTGTCTTGCGATCGGTTTAAACACAGCCGAAAATCC
CCAAATTTGGAAGCGTTCCGAACTTTGGAATGTTGATCGTAGGCGTAATT
TCCAATATTCGGAAACGAACTGACCTCCGAGTAACGATTGCCAAAAAATG
AACCGTTCTAAATTTTGGAGTGGTGTCTTTTGAAGATTACTGCTGCCGAG
GGCTTATACGAACACCCTCTTGTTGAATAGGAGGTTCTTGGGGACCTTCT
TAGGAAGGTGTATTACAAAAACATCAATATCACACCGGCTACGAGCCACT
TTATGACCCTGAAGTCAGCATCGACTGTGAAGACCACCTATTCAATATTA
CCTAAAAATCCGAAGCACTCGTGTCGTAGCGTGGTCTGGAAAGCTTTCAA
CTTGTGGCTTGTGCTCTCTGACGGCGAGCAGGCCGCCTGAACAGTCCCAG
TTTTCTCCGGTAGTCCCTCCCACGCCCATCGTCACTGTAACGCCCACGGG
CCATATAGCCTTTTTGCACTAAACTTGGCATGTCCGTTGCCATTCAGTGA
GCGGTCATGGGGCTTAAAGAGGGTGGGTCAATATTTTCTCTACACAGCCG
AAGTCATCGATGCTTGCATTGAACTGATCCGTTGGGGAAAGGAGCTTCCC
GGTCAAACCACTCTAATCGAGCATTTACTGTTTATACTATTTGTCTAATT
GCTGAAACACTGTTTTAAGCTAGTAATATTTGTCAAACTCTAGACCCTTC
GTTTCCAATTTTTCAGCTTTTGGAAGATAAGCTTGTGCGTCTAAACCGAA
CTTTACGACTCAGTATAAGTAGCGGCATTTTTCCCGAGTACACTATCTTT
GTCATCATTTGAGCAATCCGACAATAGGGGGGGGGGAAGGGATGGGGGAA
AATATTCCCTATTTCAGGGGGGGGTCGTCAAAAGAGCCCCTCCCGGGGAC
ATATTTTGCACATCCCCTCCCCTCCTTTTCGTTGTATCGCTCATTTCTTA
CACTGGTACGAGTTGTACTGGCAGTACGAAAGTCAGATTGTATTTTATGT
ATGGTCAGCACTTTCAGCAGAGTGTGGATCAACCGTGTATGGTTGCCAAT
CCTGCTCGTGATCAGCTGAACAGGGAAAACATTTTTTCCCCTGTCCCCGT
TCTCGCCTGAGAATTTGGTCTTGCGAGACGGGTTCGGTCGTACCGCCCCG
CGTCAGCCCGCCCATTCTCCACACTCAGGCTGAATCTGATTGGTTAATGC
TTACTCACGGGATTCCTCCCACTTTCCGCGACGGCGTCCATTTATTTATT
CAAACCGTCAACCGCCATCGAGTCAGTCCCGAATTTATCAGATCACGCAA
TTGCGTATCGATGGCGTTCACTGCCGAGAGTCCGCCGGCACAGTAGGCCA
GCAGTCCTCGGGGCATTTCGAGTAACGGATGCTGCCTTTTCAGTTTTCAC
CCAAGCATCAATTTTTCGTTCCTCTCTTTTCCCACACCCACTATTGGCAA
AAGAAGCATGTAGGTAGGTGAACGCAAAAACGCGTTCACAGAGACCCTGT
CGCACTTGTGGAGTTCTGCTCACGTTGACCACGGCACATTTTGATCGGTA
CATATTCCCACACCGTTTTCGGCGATTACTCCTCGAACCAAGTGTTGGCT
ACATAGAGAGTAGTCTCTAGTAGTGGTGCTCTTATTACACCTCTAACCTC
CGACACGCATGACGGATGCAGAGCCTGATGGTGGGGCATTGCAAGTATCA
CGGTGGTGGTTTAACGCAGAGGCAAACGGGACTTTTCGATACTCAAAGCG
CTGTTTCTTGGGGACACAAATCCCCGAATTACGTTATACGCTCCTCTTTT
TTTCTTGTTGTGCGAAATGACACGAGCCCTGTTAGGCCCCTCTTCCTCCG
AGGGACATGTGAAGAGGGAAAAGCAGGCGATACGAATAATTCTACGCCAT
CATTTGAATGCTACCAACCGCAGTTTTGTACGTAGGGTACTGTAATTACT
TACACATTTTTGTTGTGCTAATAAAATAGTGTTTGTACCGCCAAAACGAA
AAAACTCACGAGGCCTTTTAACTCTCCCCGCCCTCCCCGCCTCTCACAAC
CGCAAAACACTCTCGATCCCGCAACATCTCTCACAGCTACAGCGAAGGAT
GCCGGCAGCGAAAAACAGGAGGGCGCACGCAAGGGAGACGGCGACCTAGG
CGGGCTTCCGACGGCAGAGGGCGTTTTCGTCGGGGATCCCGTCGCGCAGT
CTCTCTACCCCAACCCCGCTCCTGTTATCACTCCAGTGTCTCACCAATAT
AACTTGAACTCCCAAGCGAGCCAGGGACGGCCGCCGAGCGGGGTGTCTAT
CACCACCGGGGGTCATCACGTTGTGACCAGTGCTGCTGGTGGAATGCACC
ATACGGTGTTCTTGGTCGACCCGCGCGGATTCTACGCGTGGGGGCGCGTG
CCGCAAGCGTTCGTGTGCAACAGCTGCGGATTCTCCGGGATGACAAGTGC
TTTCACGGTAAGGAGCGCTGTAGGCCTGGCGAAACGGAGTTGGCGAAGCT
ACATACTTTTCACTGTTTTGACCGCTGCGTTTGAACCTGATCCTCTTCGC
TGAATCCCGCGTTTTCGAGAGTCAAGTGCTTACAAGTAGCGGTGGGTTGC
GCCCGAGATCGAAGAAAACGACGTTCTAGAAGCAGCAGTACACATTTTTC
TCCCTCGTCAACGAAAGATGCTTGAACGGCTCCTTGAGGAAGTACACCAC
ACAAGCGAAGCCTTGGGCACCCGAGAAGGGTAGTTCCTCGGGGAGCATTG
GCGCTAATAACAAGTCTATCGTAATCGTTTCTTCTCTCGATTGACTCTTT
CTCTTCTCCATCCCGATCGTGCGATTCAACCGTTTGGCGTCCCAGGACCC
TTGCTCTAATGCCCCCATCCTGGCAGCGGCGTGCTGTTGCCTCGTGGGAT
TCTGGCCCTGCTCCTTCATCCCCTTCCTCATCCCGAGTCTAAGGGACACG
GTGCACCAGTGCCCGGGGTGCGGACTTACCGTGGGCAGGTCCCAGCAGTT
CCGCTGAAGCCGTCACGGTTTCGACCGATCGCTCCAAACGACTCGAGCAA
TCGAATGTTAACAACTATGTACGCACGTAAATCGGTTAGACTATGACAGC
GGGTCACAGATAATCTAGGCATTAAGTTCGTCTTTTGAAGTTCTACTGTA
CATAA
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Coding sequence (CDS) from alignment at A-nodosum_M_contig1124:162950..169854-

>mRNA_A-nodosum_M_contig1124.18.1 ID=mRNA_A-nodosum_M_contig1124.18.1|Name=mRNA_A-nodosum_M_contig1124.18.1|organism=Ascophyllum nodosum dioecious|type=CDS|length=696bp|location=Sequence derived from alignment at A-nodosum_M_contig1124:162950..169854- (Ascophyllum nodosum dioecious)
ATGCCTCGCGCAGACGGCTACAAGACCCTTCCCGATGATAACGATGCAGG
CGAGCGTACGGAAGACCGAGGTGAGGCGGAGGCTGACGGCGCTCCCACAC
GGCGAGAGGGCACGGCCGCTGGTGCCGCACCATCGTCGGCTGGTGCGGGA
TCAACAGAAGCAGCTACAGCGAAGGATGCCGGCAGCGAAAAACAGGAGGG
CGCACGCAAGGGAGACGGCGACCTAGGCGGGCTTCCGACGGCAGAGGGCG
TTTTCGTCGGGGATCCCGTCGCGCAGTCTCTCTACCCCAACCCCGCTCCT
GTTATCACTCCAGTGTCTCACCAATATAACTTGAACTCCCAAGCGAGCCA
GGGACGGCCGCCGAGCGGGGTGTCTATCACCACCGGGGGTCATCACGTTG
TGACCAGTGCTGCTGGTGGAATGCACCATACGGTGTTCTTGGTCGACCCG
CGCGGATTCTACGCGTGGGGGCGCGTGCCGCAAGCGTTCGTGTGCAACAG
CTGCGGATTCTCCGGGATGACAAGTGCTTTCACGGACCCTTGCTCTAATG
CCCCCATCCTGGCAGCGGCGTGCTGTTGCCTCGTGGGATTCTGGCCCTGC
TCCTTCATCCCCTTCCTCATCCCGAGTCTAAGGGACACGGTGCACCAGTG
CCCGGGGTGCGGACTTACCGTGGGCAGGTCCCAGCAGTTCCGCTGA
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