mRNA_3100 (mRNA) Tribonema minus UTEX_B_3156

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
Namejgi.p|Trimin1|161148
Unique NamemRNA_3100
TypemRNA
OrganismTribonema minus UTEX_B_3156 (Tribonema minus UTEX_B_3156 )
Homology
BLAST of jgi.p|Trimin1|161148 vs. uniprot
Match: A0A836CJ84_9STRA (Leucine carboxyl methyltransferase-domain-containing protein (Fragment) n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CJ84_9STRA)

HSP 1 Score: 451 bits (1160), Expect = 4.010e-160
Identity = 233/233 (100.00%), Postives = 233/233 (100.00%), Query Frame = 1
Query:    1 MPPDPLRTLLAGETEVTALRRDLYAVRAERGAAAARNALEAVAVRRVWSTGRLAAALRCGPGQVVFFGCGMDTRPRRLLVPGLAIYEVETAAALGAKEALLSRAPYGSFSSVPTPVAADLERPPEWQAALASAGFDASRPAVWVVEDVLCRLAWGEAQAVLQAAARGPLGTTVIADCPTPATAELGRFGVRCPETCLSALGFATCEASQLGDPSANFGGLDASAPPVYVPGGW 699
            MPPDPLRTLLAGETEVTALRRDLYAVRAERGAAAARNALEAVAVRRVWSTGRLAAALRCGPGQVVFFGCGMDTRPRRLLVPGLAIYEVETAAALGAKEALLSRAPYGSFSSVPTPVAADLERPPEWQAALASAGFDASRPAVWVVEDVLCRLAWGEAQAVLQAAARGPLGTTVIADCPTPATAELGRFGVRCPETCLSALGFATCEASQLGDPSANFGGLDASAPPVYVPGGW
Sbjct:    1 MPPDPLRTLLAGETEVTALRRDLYAVRAERGAAAARNALEAVAVRRVWSTGRLAAALRCGPGQVVFFGCGMDTRPRRLLVPGLAIYEVETAAALGAKEALLSRAPYGSFSSVPTPVAADLERPPEWQAALASAGFDASRPAVWVVEDVLCRLAWGEAQAVLQAAARGPLGTTVIADCPTPATAELGRFGVRCPETCLSALGFATCEASQLGDPSANFGGLDASAPPVYVPGGW 233          
BLAST of jgi.p|Trimin1|161148 vs. uniprot
Match: A0A7K2T7H6_9ACTN (S-adenosyl-L-methionine-dependent methyltransferase n=1 Tax=Streptomyces sp. SID8352 TaxID=2690338 RepID=A0A7K2T7H6_9ACTN)

HSP 1 Score: 62.8 bits (151), Expect = 4.370e-8
Identity = 66/199 (33.17%), Postives = 89/199 (44.72%), Query Frame = 1
Query:  124 VAVRRVWSTGRLAAALR-CGPGQVVFFGCGMDTRPRRLLVP-GLAIYEVETAAALGAKEALLSRAPYGSFSSVPT-PVAADLERPPEWQAALASAGFDASRPAVWVVEDVLCRLAWGEAQAVLQ-------------------AAARGPLGTTVIADCPTPATAELGRFGVRCPETCLSALGFATCEASQLGDPSANFG 654
            +A+R  +    +A  LR  G  Q+V    GMDTR  RL  P G  +YEV+ AA +  K   L R   G+ +      V ADL    +W  ALA+AGFDASRP VW+ E +L  L   +A  +L+                   A  R P   + +       T    RFG   PE  L+  G+ T E  + G+P A  G
Sbjct:   56 IAIRTRYFDDAVADVLRETGADQIVLVAAGMDTRAFRLDWPAGATVYEVDHAALIDEKRERLRR--LGAEARADRREVRADLAG--DWLPALAAAGFDASRPTVWITEGLLFFLTEDQAAGLLRTLGSVSAPGSRLVVDFVSRALLRSPFSQSFLRGLREDGTPW--RFGTDEPERFLAGTGWKTHEVREPGEPGAGEG 248          
BLAST of jgi.p|Trimin1|161148 vs. uniprot
Match: A0A4R1HH28_9PSEU (S-adenosyl-L-methionine-dependent methyltransferase n=2 Tax=Pseudonocardia endophytica TaxID=401976 RepID=A0A4R1HH28_9PSEU)

HSP 1 Score: 62.8 bits (151), Expect = 5.020e-8
Identity = 41/98 (41.84%), Postives = 57/98 (58.16%), Query Frame = 1
Query:  157 LAAALRCGPGQVVFFGCGMDTRPRRLLVP-GLAIYEVETAAALGAKEALLSRAPYGSFSSVPTPVAADLERPPEWQAALASAGFDASRPAVWVVEDVL 447
            L +A+  G  QVV    G+DTR  RL  P G+ +YEV+  A L  K+ +L+ A     +S  T VA  ++   +W AAL +AGFD SRP VW+ E +L
Sbjct:   84 LGSAVEAGTSQVVLLAAGLDTRGCRLGWPSGVTVYEVDQPAVLEFKDRVLADA---GATSEATRVAVPVDLRHDWPAALTAAGFDPSRPTVWLAEGLL 178          
BLAST of jgi.p|Trimin1|161148 vs. uniprot
Match: UPI0011775E9E (SAM-dependent methyltransferase n=1 Tax=Crossiella equi TaxID=130796 RepID=UPI0011775E9E)

HSP 1 Score: 61.6 bits (148), Expect = 5.670e-8
Identity = 46/110 (41.82%), Postives = 62/110 (56.36%), Query Frame = 1
Query:  124 VAVRRVWSTGRLAAALRCGPGQVVFFGCGMDTRPRRLLVPG-LAIYEVETAAALGAKEALLSRAPYGSFSSVPTPVA-ADLERPPEWQAALASAGFDASRPAVWVVEDVL 447
            +A R V+    L AAL  G  QVV  G G+DTR  R+ +PG   +YEV+T A    K  +L     G+  +V   V  ADL    +W AAL +AGFD +RP +WV+E +L
Sbjct:   81 LAARTVYYDRALLAALSQGTRQVVLPGAGLDTRAYRMALPGDTTLYEVDTPAVHDFKTTVLD----GTEPAVRRVVVEADLRA--DWAAALTAAGFDRTRPTIWVLEGLL 184          
BLAST of jgi.p|Trimin1|161148 vs. uniprot
Match: UPI001AEA8863 (SAM-dependent methyltransferase n=1 Tax=Crossiella equi TaxID=130796 RepID=UPI001AEA8863)

HSP 1 Score: 61.6 bits (148), Expect = 1.280e-7
Identity = 46/110 (41.82%), Postives = 62/110 (56.36%), Query Frame = 1
Query:  124 VAVRRVWSTGRLAAALRCGPGQVVFFGCGMDTRPRRLLVPG-LAIYEVETAAALGAKEALLSRAPYGSFSSVPTPVA-ADLERPPEWQAALASAGFDASRPAVWVVEDVL 447
            +A R V+    L AAL  G  QVV  G G+DTR  R+ +PG   +YEV+T A    K  +L     G+  +V   V  ADL    +W AAL +AGFD +RP +WV+E +L
Sbjct:   81 LAARTVYYDRALLAALSQGTRQVVLPGAGLDTRAYRMALPGDTTLYEVDTPAVHDFKTTVLD----GTEPAVRRVVVEADLRA--DWAAALTAAGFDRTRPTIWVLEGLL 184          
BLAST of jgi.p|Trimin1|161148 vs. uniprot
Match: A0A2N8P0N4_STREU (S-adenosyl-L-methionine-dependent methyltransferase n=1 Tax=Streptomyces eurocidicus TaxID=66423 RepID=A0A2N8P0N4_STREU)

HSP 1 Score: 59.3 bits (142), Expect = 7.000e-7
Identity = 58/176 (32.95%), Postives = 77/176 (43.75%), Query Frame = 1
Query:  187 QVVFFGCGMDTRPRRLLVP-GLAIYEVETAAALGAKEALLSRAPYGSFSSVPTPVAADLERPPEWQAALASAGFDASRPAVWVVEDVLCRL-------------------AWGEAQAVLQAAARGPLGTTVIADCPTPATAELGRFGVRCPETCLSALGFATCEASQLGDPSANFG 654
            QVV    GMDTR  RL  P G  +YE++ AA +  K A L+R    + ++    V ADL    +W  AL  AGFD SRP +WV E ++  L                   +W     V +A  R P   T +       T  L  FG   PE  LSA G+   +  + G+P A  G
Sbjct:   77 QVVLVAAGMDTRAFRLPWPAGTTVYELDHAALVDEKRARLARLD-ATATTDRREVRADLAE--DWLPALTRAGFDPSRPTLWVAEGLMFFLTREQSAGLLRALGAASAPGSWLAVDFVSKALLRSPFSQTFLKGLKEDGTPWL--FGTDEPEEFLSAAGWKVRDLKEPGEPGAGEG 247          
BLAST of jgi.p|Trimin1|161148 vs. uniprot
Match: A0A8I1SVR8_THIA3 (S-adenosyl-L-methionine-dependent methyltransferase n=3 Tax=Thiomonas TaxID=32012 RepID=A0A8I1SVR8_THIA3)

HSP 1 Score: 58.9 bits (141), Expect = 9.530e-7
Identity = 53/141 (37.59%), Postives = 72/141 (51.06%), Query Frame = 1
Query:  118 EAVAVRRVWSTGRLAAALRCGPG--QVVFFGCGMDTRPRRLLVP-GLAIYEVETAAALGAKEALLSRAPYGSFSSVPTPVAADLERPPEWQAALASAGFDASRPAVWVVEDVLCRLAWGEAQAVLQAA-ARGPLGTTVIAD 528
            E V +R  +     AAALR  P   Q+V    GMDTR  RL  P G  +YEV+    L  K A L +   G   ++   VAADL R  +W+  L  AGFD S+PA+W+ E +L  L   +A  VL    A   +G+ ++ D
Sbjct:   54 EFVVIRTRFFDDACAAALREEPAIRQIVMVAAGMDTRAYRLDWPQGTKVYEVDHGELLAEKTARLHQIG-GPSRALRLNVAADLAR--DWRGPLIGAGFDPSKPALWLAEGLLFFLTDEQAGEVLSTCRAMSAMGSRLVVD 191          
BLAST of jgi.p|Trimin1|161148 vs. uniprot
Match: A0A1Z2KVI2_9ACTN (S-adenosyl-L-methionine-dependent methyltransferase n=2 Tax=Streptomyces albireticuli TaxID=1940 RepID=A0A1Z2KVI2_9ACTN)

HSP 1 Score: 58.5 bits (140), Expect = 1.300e-6
Identity = 62/198 (31.31%), Postives = 85/198 (42.93%), Query Frame = 1
Query:  124 VAVRRVWSTGRLAAALRCGP-GQVVFFGCGMDTRPRRLLVP-GLAIYEVETAAALGAKEALLSRAPYGSFSSVPTPVAADLERPPEWQAALASAGFDASRPAVWVVEDVLCRL-------------------AWGEAQAVLQAAARGPLGTTVIADCPTPATAELGRFGVRCPETCLSALGFATCEASQLGDPSANFG 654
            +A+R  +    +A  LR     QVV    GMDTR  RL  P G  +YE++  A +  K A L+R    + ++    V ADL    EW  AL  AGFD SRP +WV E ++  L                   +W     V +A  R P   T +       T  L  FG   PE  L+A G+   +  + G+P A  G
Sbjct:   55 IAIRTKYFDDSIATVLRENTIEQVVLVAAGMDTRAFRLTWPAGTTVYEMDHHALIDEKRARLARLD-ATATADRREVKADLAE--EWLPALTRAGFDPSRPTLWVTEGLMFFLTRDQAAQLLRSLGAASAPGSWLAVDFVSKALLRSPFSQTFLKGLKEDGTPWL--FGTDEPEEFLAATGWKARDVREPGEPGAGEG 247          
BLAST of jgi.p|Trimin1|161148 vs. uniprot
Match: UPI001675BAD5 (S-adenosyl-L-methionine-dependent methyltransferase n=2 Tax=Streptomyces lucensis TaxID=67319 RepID=UPI001675BAD5)

HSP 1 Score: 57.8 bits (138), Expect = 2.570e-6
Identity = 49/120 (40.83%), Postives = 64/120 (53.33%), Query Frame = 1
Query:  127 AVRRVWSTGRLAAALRCGPGQVVFFGCGMDTRPRRLLVP-GLAIYEVETAAALGAKEALLSRAPYGSFSSVPTPVAADLERPPEWQAALASAGFDASRPAVWVVEDVLCRLAWGEAQAVL 483
            AVR  +    + AA+R G  QVV    G+D R  RL +P G   +EV+       K+ALL R+     S     VAADL    +W++AL +AGFD SRP VW+VE +L  L      AVL
Sbjct:   73 AVRTRFFDDEVLAAVRRGIRQVVTLAAGLDGRGVRLELPSGTRWFEVDLPEMTEFKDALLERSGL-PLSCERRAVAADLRE--DWRSALRAAGFDPSRPTVWLVEGLLMYLTDEAGDAVL 189          
BLAST of jgi.p|Trimin1|161148 vs. uniprot
Match: UPI00146C35E3 (SAM-dependent methyltransferase n=1 Tax=Streptomyces sp. HNM0574 TaxID=2714954 RepID=UPI00146C35E3)

HSP 1 Score: 54.7 bits (130), Expect = 2.740e-5
Identity = 58/180 (32.22%), Postives = 74/180 (41.11%), Query Frame = 1
Query:  178 GPGQVVFFGCGMDTRPRRL-LVPGLAIYEVETAAALGAKEALLSRAPYGSFSSVPT-PVAADLERPPEWQAALASAGFDASRPAVWVVEDVLCRLAWGEAQAVL-------------------QAAARGPLGTTVIADCPTPATAELGRFGVRCPETCLSALGFATCEASQLGDPSANFG 654
            G  QVV    GMDTR  RL   PG+ +YEV+  A +  K A L R   G+ +      V ADL    EW   L  AGFD SRP +WV E +   L   +A  +L                   +A  R P     +       T    RFG   PE  L+  G+   E  + G+P A  G
Sbjct:   75 GVRQVVLVAAGMDTRAFRLGWPPGVTVYEVDHEALVREKRARLER--LGATARTERREVGADLAG--EWLPRLEEAGFDRSRPVLWVAEGLFFFLTREQATGLLRLLRSASAPGSALATDFASEALLRSPFSRRFLEHLRADGTPW--RFGTDEPEEFLAGSGWKAVEVKEPGEPGAGRG 248          
The following BLAST results are available for this feature:
BLAST of jgi.p|Trimin1|161148 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 13
Match NameE-valueIdentityDescription
A0A836CJ84_9STRA4.010e-160100.00Leucine carboxyl methyltransferase-domain-containi... [more]
A0A7K2T7H6_9ACTN4.370e-833.17S-adenosyl-L-methionine-dependent methyltransferas... [more]
A0A4R1HH28_9PSEU5.020e-841.84S-adenosyl-L-methionine-dependent methyltransferas... [more]
UPI0011775E9E5.670e-841.82SAM-dependent methyltransferase n=1 Tax=Crossiella... [more]
UPI001AEA88631.280e-741.82SAM-dependent methyltransferase n=1 Tax=Crossiella... [more]
A0A2N8P0N4_STREU7.000e-732.95S-adenosyl-L-methionine-dependent methyltransferas... [more]
A0A8I1SVR8_THIA39.530e-737.59S-adenosyl-L-methionine-dependent methyltransferas... [more]
A0A1Z2KVI2_9ACTN1.300e-631.31S-adenosyl-L-methionine-dependent methyltransferas... [more]
UPI001675BAD52.570e-640.83S-adenosyl-L-methionine-dependent methyltransferas... [more]
UPI00146C35E32.740e-532.22SAM-dependent methyltransferase n=1 Tax=Streptomyc... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Contig_16contigContig_16:1427699..1428397 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Tribonema minus UTEX_B_31562021-06-17
Properties
Property NameValue
ProteomeIdTrimin1|161148|e_gw1.16.129.1
DescriptionLeucine carboxyl methyltransferase
COG categoryQ
Max annot lvl35493|Streptophyta
EggNOG OGsCOG3315@1|root,2QRNU@2759|Eukaryota,37Q2S@33090|Viridiplantae,3GFU7@35493|Streptophyta,3KR4R@4447|Liliopsida,3I9JN@38820|Poales
Evalue1.11e-13
Seed ortholog15368.BRADI1G07120.1
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionS-adenosyl-L-methionine-dependent methyltransferase
Ec32 orthologEc-28_002500.1
TranscriptId161470
TrackFilteredModels1
ProteinId161148
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameSpeciesTypePosition
jgi.p|Trimin1|161148gene_3100Tribonema minus UTEX_B_3156 geneContig_16 1427699..1428397 -


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
exon_3100_1exon_3100_1Tribonema minus UTEX_B_3156 exonContig_16 1427699..1428397 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
CDS_3100CDS_3100Tribonema minus UTEX_B_3156 CDSContig_16 1427699..1428397 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
jgi.p|Trimin1|161148Trimin1|161148|e_gw1.16.129.1Tribonema minus UTEX_B_3156 polypeptideContig_16 1427699..1428397 -


Sequences
The following sequences are available for this feature:

spliced messenger RNA

>mRNA_3100 ID=mRNA_3100|Name=jgi.p|Trimin1|161148|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=699bp|location=Sequence derived from alignment at Contig_16:1427699..1428397- (Tribonema minus UTEX_B_3156 )|Notes=Excludes all bases but those of type(s): exon.  
ATGCCGCCCGACCCCCTGCGGACGCTGCTGGCGGGGGAGACCGAGGTCAC
GGCGCTGCGGCGCGACCTCTACGCCGTCAGGGCGGAGCGCGGCGCGGCCG
CCGCGAGGAACGCGCTGGAGGCCGTCGCCGTGCGGCGCGTCTGGTCGACG
GGGCGACTGGCGGCCGCGCTGCGATGCGGGCCCGGGCAGGTCGTCTTCTT
CGGCTGCGGCATGGACACGCGCCCCCGCAGGCTGCTGGTGCCGGGTCTGG
CGATCTACGAAGTCGAGACGGCGGCGGCGCTGGGAGCGAAGGAGGCGCTG
CTGTCGCGCGCGCCGTACGGATCGTTCTCGAGCGTCCCGACGCCCGTCGC
GGCGGACCTCGAGCGCCCTCCCGAATGGCAGGCCGCCCTGGCGTCCGCCG
GCTTCGACGCGTCGCGACCTGCCGTCTGGGTCGTCGAGGACGTCCTGTGT
CGGCTGGCGTGGGGGGAGGCCCAGGCGGTGCTGCAGGCCGCCGCCCGGGG
CCCCCTGGGGACGACGGTCATCGCCGACTGCCCGACGCCGGCGACGGCGG
AGCTCGGACGGTTCGGCGTGCGCTGCCCGGAGACCTGCCTGTCCGCGCTG
GGATTCGCGACGTGCGAGGCGTCGCAACTGGGGGACCCGAGCGCCAACTT
CGGCGGTCTGGACGCGTCCGCTCCGCCGGTCTACGTCCCCGGCGGGTGG
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protein sequence of jgi.p|Trimin1|161148

>Trimin1|161148|e_gw1.16.129.1 ID=Trimin1|161148|e_gw1.16.129.1|Name=jgi.p|Trimin1|161148|organism=Tribonema minus UTEX_B_3156 |type=polypeptide|length=233bp
MPPDPLRTLLAGETEVTALRRDLYAVRAERGAAAARNALEAVAVRRVWST
GRLAAALRCGPGQVVFFGCGMDTRPRRLLVPGLAIYEVETAAALGAKEAL
LSRAPYGSFSSVPTPVAADLERPPEWQAALASAGFDASRPAVWVVEDVLC
RLAWGEAQAVLQAAARGPLGTTVIADCPTPATAELGRFGVRCPETCLSAL
GFATCEASQLGDPSANFGGLDASAPPVYVPGGW
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mRNA from alignment at Contig_16:1427699..1428397-

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_3100 ID=mRNA_3100|Name=jgi.p|Trimin1|161148|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=699bp|location=Sequence derived from alignment at Contig_16:1427699..1428397- (Tribonema minus UTEX_B_3156 )
ATGCCGCCCGACCCCCTGCGGACGCTGCTGGCGGGGGAGACCGAGGTCAC GGCGCTGCGGCGCGACCTCTACGCCGTCAGGGCGGAGCGCGGCGCGGCCG CCGCGAGGAACGCGCTGGAGGCCGTCGCCGTGCGGCGCGTCTGGTCGACG GGGCGACTGGCGGCCGCGCTGCGATGCGGGCCCGGGCAGGTCGTCTTCTT CGGCTGCGGCATGGACACGCGCCCCCGCAGGCTGCTGGTGCCGGGTCTGG CGATCTACGAAGTCGAGACGGCGGCGGCGCTGGGAGCGAAGGAGGCGCTG CTGTCGCGCGCGCCGTACGGATCGTTCTCGAGCGTCCCGACGCCCGTCGC GGCGGACCTCGAGCGCCCTCCCGAATGGCAGGCCGCCCTGGCGTCCGCCG GCTTCGACGCGTCGCGACCTGCCGTCTGGGTCGTCGAGGACGTCCTGTGT CGGCTGGCGTGGGGGGAGGCCCAGGCGGTGCTGCAGGCCGCCGCCCGGGG CCCCCTGGGGACGACGGTCATCGCCGACTGCCCGACGCCGGCGACGGCGG AGCTCGGACGGTTCGGCGTGCGCTGCCCGGAGACCTGCCTGTCCGCGCTG GGATTCGCGACGTGCGAGGCGTCGCAACTGGGGGACCCGAGCGCCAACTT CGGCGGTCTGGACGCGTCCGCTCCGCCGGTCTACGTCCCCGGCGGGTGG
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Coding sequence (CDS) from alignment at Contig_16:1427699..1428397-

>mRNA_3100 ID=mRNA_3100|Name=jgi.p|Trimin1|161148|organism=Tribonema minus UTEX_B_3156 |type=CDS|length=699bp|location=Sequence derived from alignment at Contig_16:1427699..1428397- (Tribonema minus UTEX_B_3156 )
ATGCCGCCCGACCCCCTGCGGACGCTGCTGGCGGGGGAGACCGAGGTCAC
GGCGCTGCGGCGCGACCTCTACGCCGTCAGGGCGGAGCGCGGCGCGGCCG
CCGCGAGGAACGCGCTGGAGGCCGTCGCCGTGCGGCGCGTCTGGTCGACG
GGGCGACTGGCGGCCGCGCTGCGATGCGGGCCCGGGCAGGTCGTCTTCTT
CGGCTGCGGCATGGACACGCGCCCCCGCAGGCTGCTGGTGCCGGGTCTGG
CGATCTACGAAGTCGAGACGGCGGCGGCGCTGGGAGCGAAGGAGGCGCTG
CTGTCGCGCGCGCCGTACGGATCGTTCTCGAGCGTCCCGACGCCCGTCGC
GGCGGACCTCGAGCGCCCTCCCGAATGGCAGGCCGCCCTGGCGTCCGCCG
GCTTCGACGCGTCGCGACCTGCCGTCTGGGTCGTCGAGGACGTCCTGTGT
CGGCTGGCGTGGGGGGAGGCCCAGGCGGTGCTGCAGGCCGCCGCCCGGGG
CCCCCTGGGGACGACGGTCATCGCCGACTGCCCGACGCCGGCGACGGCGG
AGCTCGGACGGTTCGGCGTGCGCTGCCCGGAGACCTGCCTGTCCGCGCTG
GGATTCGCGACGTGCGAGGCGTCGCAACTGGGGGACCCGAGCGCCAACTT
CGGCGGTCTGGACGCGTCCGCTCCGCCGGTCTACGTCCCCGGCGGGTGG
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