mRNA_2201 (mRNA) Tribonema minus UTEX_B_3156

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
Namejgi.p|Trimin1|158516
Unique NamemRNA_2201
TypemRNA
OrganismTribonema minus UTEX_B_3156 (Tribonema minus UTEX_B_3156 )
Homology
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BLAST of jgi.p|Trimin1|158516 vs. uniprot
Match: A0A836CB68_9STRA (DNA-directed RNA polymerase subunit n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CB68_9STRA)

HSP 1 Score: 248 bits (633), Expect = 1.010e-83
Identity = 112/112 (100.00%), Postives = 112/112 (100.00%), Query Frame = 1 
Query:    1 MLFCPTCGNLLMVEAATRGGQRFACQTCPYVHPLGQGGKVTKALTLQRKEVDDVLGGADAWVNVDKAPVACADCKDPGKGKEAYFFQAQLRSADEPMTVFYKCVDCGFRWNE 336
            MLFCPTCGNLLMVEAATRGGQRFACQTCPYVHPLGQGGKVTKALTLQRKEVDDVLGGADAWVNVDKAPVACADCKDPGKGKEAYFFQAQLRSADEPMTVFYKCVDCGFRWNE
Sbjct:    1 MLFCPTCGNLLMVEAATRGGQRFACQTCPYVHPLGQGGKVTKALTLQRKEVDDVLGGADAWVNVDKAPVACADCKDPGKGKEAYFFQAQLRSADEPMTVFYKCVDCGFRWNE 112
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BLAST of jgi.p|Trimin1|158516 vs. uniprot
Match: D7G5N9_ECTSI (DNA-directed RNA polymerase subunit n=2 Tax=Ectocarpus TaxID=2879 RepID=D7G5N9_ECTSI)

HSP 1 Score: 147 bits (370), Expect = 1.140e-43
Identity = 63/112 (56.25%), Postives = 78/112 (69.64%), Query Frame = 1 
Query:    1 MLFCPTCGNLLMVEAATRGGQRFACQTCPYVHPLGQGGKVTKALTLQRKEVDDVLGGADAWVNVDKAPVACADCKDPGKGKEAYFFQAQLRSADEPMTVFYKCVDCGFRWNE 336
            MLFCPT G+LLMVE+  +G  RF CQTCPY+HP+    K+T+ + L++K VDD++GG DAW NVDK    C  C +    KEA+F   QLRSADEPMT FYKC +C  RW E
Sbjct:    1 MLFCPTDGSLLMVESGPQGWTRFCCQTCPYIHPMEPSDKITRKIALKKKAVDDIMGGDDAWANVDKTQTTCPFCNN----KEAFFLMVQLRSADEPMTCFYKCTNCKQRWKE 108
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BLAST of jgi.p|Trimin1|158516 vs. uniprot
Match: A0A397H395_9GLOM (DNA-directed RNA polymerase subunit n=1 Tax=Diversispora epigaea TaxID=1348612 RepID=A0A397H395_9GLOM)

HSP 1 Score: 141 bits (355), Expect = 2.640e-41
Identity = 67/117 (57.26%), Postives = 82/117 (70.09%), Query Frame = 1 
Query:    1 MLFCPTCGNLLMVEAATRGGQRFACQTCPYVHPLGQ--GGKVTKALT-LQRKEVDDVLGGADAWVNVDKAPVACADCKDPGKGKEAYFFQAQLRSADEPMTVFYKCV--DCGFRWNE 336
            MLFCP CGN+L++++ T+G Q F CQTCPYV P+G   G ++ K  T L+RKEVDDVLGG +AW NVD     C  C+ P     AYF Q Q RSADEPM++FYKC   DCGF+WNE
Sbjct:    1 MLFCPCCGNILLIKSHTQGEQSFYCQTCPYVFPIGCRIGQQIYKNRTILKRKEVDDVLGGEEAWKNVDSTDARCPKCEHP----RAYFMQIQTRSADEPMSIFYKCCNHDCGFQWNE 113
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BLAST of jgi.p|Trimin1|158516 vs. uniprot
Match: U9UHV3_RHIID (DNA-directed RNA polymerase subunit n=8 Tax=Eukaryota TaxID=2759 RepID=U9UHV3_RHIID)

HSP 1 Score: 136 bits (342), Expect = 2.220e-39
Identity = 63/114 (55.26%), Postives = 76/114 (66.67%), Query Frame = 1 
Query:    1 MLFCPTCGNLLMVEAATRGGQRFACQTCPYVHPLGQGGKVTKALTLQRKEVDDVLGGADAWVNVDKAPVACADCKDPGKGKEAYFFQAQLRSADEPMTVFYKCV--DCGFRWNE 336
            MLFCP CGNLL+++  T+G Q F CQTCPYV P+G   +     TL+RKEVDDVLGG + W NVD   V C  C+ P     AYF Q Q RSADEPM++FYKC   +C  +WNE
Sbjct:    1 MLFCPCCGNLLLIQHHTQGEQSFFCQTCPYVFPIGSK-EYKNRTTLKRKEVDDVLGGEEGWKNVDSTEVTCPKCEHP----RAYFMQIQTRSADEPMSIFYKCCNHECQHQWNE 109
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BLAST of jgi.p|Trimin1|158516 vs. uniprot
Match: A0A397VYQ6_9GLOM (DNA-directed RNA polymerase subunit n=2 Tax=Gigaspora TaxID=4873 RepID=A0A397VYQ6_9GLOM)

HSP 1 Score: 135 bits (341), Expect = 3.560e-39
Identity = 64/117 (54.70%), Postives = 77/117 (65.81%), Query Frame = 1 
Query:    1 MLFCPTCGNLLMVEAATRGGQRFACQTCPYVHPLG-QGGK--VTKALTLQRKEVDDVLGGADAWVNVDKAPVACADCKDPGKGKEAYFFQAQLRSADEPMTVFYKCV--DCGFRWNE 336
            MLFCP CGNLL+++   +G Q F CQTCPY+ P+G +GGK       TL+RKEVDDVLGG + W NVD     C  C+ P     AYF Q Q RSADEPM++FYKC   DC  +WNE
Sbjct:    1 MLFCPCCGNLLLIKTLMQGEQAFYCQTCPYIFPIGGRGGKSLYKNRTTLKRKEVDDVLGGEEGWKNVDSTEARCPKCEHP----RAYFMQIQTRSADEPMSIFYKCCNHDCQHQWNE 113
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BLAST of jgi.p|Trimin1|158516 vs. uniprot
Match: A0A7S0PIB0_CAFRO (DNA-directed RNA polymerase subunit n=2 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A7S0PIB0_CAFRO)

HSP 1 Score: 135 bits (339), Expect = 8.360e-39
Identity = 62/112 (55.36%), Postives = 76/112 (67.86%), Query Frame = 1 
Query:    1 MLFCPTCGNLLMVEAATRGGQRFACQTCPYVHPLGQGGKVTKALTLQRKEVDDVLGGADAWVNVDKAPVACADCKDPGKGKEAYFFQAQLRSADEPMTVFYKCVDCGFRWNE 336
            MLFCPTCGN+L++E     G +F C TCPY+  +    K+TK++TL+RK VDDVLGGA AW NVD+    C  C      + AYF Q Q+RSADEPMT FYKCV C  RWN+
Sbjct:   16 MLFCPTCGNMLLIEGGA--GTKFFCTTCPYIQRVTT--KITKSITLERKTVDDVLGGAAAWENVDQTDALCPACGH----RRAYFMQLQIRSADEPMTTFYKCVACSGRWND 119
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BLAST of jgi.p|Trimin1|158516 vs. uniprot
Match: A0A7S0RJ62_9CHLO (DNA-directed RNA polymerase subunit n=1 Tax=Pyramimonas obovata TaxID=1411642 RepID=A0A7S0RJ62_9CHLO)

HSP 1 Score: 133 bits (334), Expect = 3.350e-38
Identity = 63/112 (56.25%), Postives = 75/112 (66.96%), Query Frame = 1 
Query:    1 MLFCPTCGNLLMVEAATRGGQRFACQTCPYVHPLGQGGKVTKALTLQRKEVDDVLGGADAWVNVDKAPVACADCKDPGKGKEAYFFQAQLRSADEPMTVFYKCVDCGFRWNE 336
            M FCPTCG LLMVE       R+ACQTCPYV+ + +  K+ K   L+ KEVDDVLGG +AW NVD+  V+C  C D G   +AYF Q Q RSADEPMT+FYKC  C  RW +
Sbjct:    2 MTFCPTCGTLLMVEYCELTRLRYACQTCPYVYMIDR--KIVKEHVLETKEVDDVLGGDEAWANVDRTEVSCPKC-DHG---QAYFMQVQTRSADEPMTIFYKCCKCANRWKD 107
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BLAST of jgi.p|Trimin1|158516 vs. uniprot
Match: A0A1Y1VKS4_9FUNG (DNA-directed RNA polymerase subunit n=3 Tax=Neocallimastigaceae TaxID=29007 RepID=A0A1Y1VKS4_9FUNG)

HSP 1 Score: 132 bits (332), Expect = 6.970e-38
Identity = 60/110 (54.55%), Postives = 76/110 (69.09%), Query Frame = 1 
Query:    7 FCPTCGNLLMVEAATRGGQRFACQTCPYVHPLGQGGKVTKALTLQRKEVDDVLGGADAWVNVDKAPVACADCKDPGKGKEAYFFQAQLRSADEPMTVFYKCVDCGFRWNE 336
            FCP+CGN+L+++   +G Q F CQTCPYV P+ +    T+    +RKEVDDVLGGA AW NVD     C  C++   GK A+F Q Q RSADEPMT+FYKC +CGF+W E
Sbjct:    4 FCPSCGNMLLLQLNLQGIQEFYCQTCPYVFPIIR--PQTQVTQFKRKEVDDVLGGAKAWENVDSTEATCPKCEN---GK-AFFMQIQTRSADEPMTIFYKCTECGFQWRE 107
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BLAST of jgi.p|Trimin1|158516 vs. uniprot
Match: A0A024UTD8_9STRA (DNA-directed RNA polymerase subunit n=8 Tax=Saprolegniaceae TaxID=4764 RepID=A0A024UTD8_9STRA)

HSP 1 Score: 130 bits (328), Expect = 2.670e-37
Identity = 62/112 (55.36%), Postives = 75/112 (66.96%), Query Frame = 1 
Query:    1 MLFCPTCGNLLMVEAATRGGQRFACQTCPYVHPLGQGGKVTKALTLQRKEVDDVLGGADAWVNVDKAPVACADCKDPGKGKEAYFFQAQLRSADEPMTVFYKCVDCGFRWNE 336
            M FCPTCGNLLMVE  +  G RF CQTCPY++ + Q  KV   + LQRK+VDDVLGG +AW NVD+    C  C+      +AYF Q Q+RSADEP T FYKC  C  +WN+
Sbjct:    1 MHFCPTCGNLLMVEPDS-DGMRFCCQTCPYIYKINQ--KVETKVPLQRKKVDDVLGGDEAWENVDQTDTRCPFCEHT----KAYFMQIQIRSADEPSTTFYKCTGCKKQWND 105
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BLAST of jgi.p|Trimin1|158516 vs. uniprot
Match: A0A077ZVQ1_STYLE (DNA-directed RNA polymerase subunit n=2 Tax=Oxytrichidae TaxID=57506 RepID=A0A077ZVQ1_STYLE)

HSP 1 Score: 130 bits (328), Expect = 2.670e-37
Identity = 61/112 (54.46%), Postives = 73/112 (65.18%), Query Frame = 1 
Query:    1 MLFCPTCGNLLMVEAATRGGQRFACQTCPYVHPLGQGGKVTKALTLQRKEVDDVLGGADAWVNVDKAPVACADCKDPGKGKEAYFFQAQLRSADEPMTVFYKCVDCGFRWNE 336
            MLFCPTC NLL++E  T    RF C+TCPYV  + Q     K + L+RKEVDDVLGG +AW NVD+    C  C +    K AYF Q Q+RSADEPMT FYKC  C ++W E
Sbjct:    1 MLFCPTCANLLLIEK-THSDFRFFCKTCPYVFVVEQN--FEKKMQLERKEVDDVLGGKEAWENVDQIEAQCPKCNN----KRAYFMQIQIRSADEPMTTFYKCTQCSYQWRE 105
The following BLAST results are available for this feature:
BLAST of jgi.p|Trimin1|158516 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A836CB68_9STRA1.010e-83100.00DNA-directed RNA polymerase subunit n=1 Tax=Tribon... [more]
D7G5N9_ECTSI1.140e-4356.25DNA-directed RNA polymerase subunit n=2 Tax=Ectoca... [more]
A0A397H395_9GLOM2.640e-4157.26DNA-directed RNA polymerase subunit n=1 Tax=Divers... [more]
U9UHV3_RHIID2.220e-3955.26DNA-directed RNA polymerase subunit n=8 Tax=Eukary... [more]
A0A397VYQ6_9GLOM3.560e-3954.70DNA-directed RNA polymerase subunit n=2 Tax=Gigasp... [more]
A0A7S0PIB0_CAFRO8.360e-3955.36DNA-directed RNA polymerase subunit n=2 Tax=Cafete... [more]
A0A7S0RJ62_9CHLO3.350e-3856.25DNA-directed RNA polymerase subunit n=1 Tax=Pyrami... [more]
A0A1Y1VKS4_9FUNG6.970e-3854.55DNA-directed RNA polymerase subunit n=3 Tax=Neocal... [more]
A0A024UTD8_9STRA2.670e-3755.36DNA-directed RNA polymerase subunit n=8 Tax=Saprol... [more]
A0A077ZVQ1_STYLE2.670e-3754.46DNA-directed RNA polymerase subunit n=2 Tax=Oxytri... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Contig_6contigContig_6:1394563..1395977 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Tribonema minus UTEX_B_31562021-06-17
Properties
Property NameValue
ProteomeIdTrimin1|158516|e_gw1.6.11.1
BRITEbr01611,ko00000,ko00001,ko00002,ko01000,ko03021,ko03032,ko03036,ko03400
KEGG rclassRC02795
KEGG ReactionR00375,R00376,R00377,R00378,R00435,R00441,R00442,R00443
KEGG ModuleM00181,M00263
KEGG Pathwayko00230,ko00240,ko01100,ko03020,ko03030,ko03410,ko03420,ko04623,ko05166,ko05169,map00230,map00240,map01100,map03020,map03030,map03410,map03420,map04623,map05166,map05169
KEGG koko:K02326,ko:K03019
EC2.7.7.7
GOsGO:0000428,GO:0003674,GO:0003676,GO:0003677,GO:0003824,GO:0003899,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005666,GO:0005667,GO:0005669,GO:0005730,GO:0005737,GO:0005829,GO:0006139,GO:0006259,GO:0006281,GO:0006283,GO:0006289,GO:0006351,GO:0006352,GO:0006353,GO:0006354,GO:0006366,GO:0006368,GO:0006383,GO:0006384,GO:0006386,GO:0006396,GO:0006399,GO:0006725,GO:0006807,GO:0006950,GO:0006974,GO:0008033,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009304,GO:0009987,GO:0010467,GO:0016070,GO:0016591,GO:0016740,GO:0016772,GO:0016779,GO:0018130,GO:0019438,GO:0030880,GO:0031123,GO:0031974,GO:0031981,GO:0032774,GO:0032991,GO:0033554,GO:0034062,GO:0034470,GO:0034641,GO:0034645,GO:0034654,GO:0034660,GO:0042779,GO:0042780,GO:0042797,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043628,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044271,GO:0044422,GO:0044424,GO:0044428,GO:0044444,GO:0044446,GO:0044451,GO:0044464,GO:0044798,GO:0046483,GO:0050896,GO:0051716,GO:0055029,GO:0061695,GO:0070013,GO:0071704,GO:0090304,GO:0090305,GO:0090501,GO:0090575,GO:0097159,GO:0097659,GO:0097747,GO:0098781,GO:0140098,GO:1901360,GO:1901362,GO:1901363,GO:1901576,GO:1902494,GO:1990234
Preferred namePOLR3K
Descriptiontermination of RNA polymerase III transcription
COG categoryK
Max annot lvl2759|Eukaryota
EggNOG OGsCOG1594@1|root,KOG2906@2759|Eukaryota
Evalue1.87e-44
Seed ortholog2880.D7G5N9
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionZinc finger, TFIIS-type
Ec32 orthologEc-12_008560.1
TranscriptId158838
TrackFilteredModels1
ProteinId158516
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameSpeciesTypePosition
jgi.p|Trimin1|158516gene_2201Tribonema minus UTEX_B_3156 geneContig_6 1394563..1395977 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
jgi.p|Trimin1|158516Trimin1|158516|e_gw1.6.11.1Tribonema minus UTEX_B_3156 polypeptideContig_6 1394563..1395977 -


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
exon_2201_2exon_2201_2Tribonema minus UTEX_B_3156 exonContig_6 1394563..1394677 -
exon_2201_1exon_2201_1Tribonema minus UTEX_B_3156 exonContig_6 1395754..1395977 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
CDS_2201CDS_2201Tribonema minus UTEX_B_3156 CDSContig_6 1395754..1395977 -


Sequences
The following sequences are available for this feature:

spliced messenger RNA

>mRNA_2201 ID=mRNA_2201|Name=jgi.p|Trimin1|158516|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=339bp|location=Sequence derived from alignment at Contig_6:1394563..1395977- (Tribonema minus UTEX_B_3156 )|Notes=Excludes all bases but those of type(s): exon.  
ATGCTCTTCTGCCCCACGTGCGGCAACCTGCTCATGGTGGAGGCGGCGAC
GCGCGGCGGGCAGCGCTTTGCGTGCCAGACGTGCCCGTACGTGCACCCGC
TCGGGCAGGGCGGCAAGGTGACCAAGGCGCTCACGCTGCAGCGCAAGGAG
GTGGACGACGTGCTCGGCGGCGCGGACGCGTGGGTCAACGTCGACAAGGC
GCCCGTGGCGTGCGCCGACTGCAAGGATCCGGGCAAGGGCAAGGAGGCCT
ACTTCTTCCAGGCGCAGCTCAGATCCGCTGACGAGCCCATGACCGTGTTC
TACAAATGCGTCGACTGTGGCTTCCGCTGGAATGAATGA
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protein sequence of jgi.p|Trimin1|158516

>Trimin1|158516|e_gw1.6.11.1 ID=Trimin1|158516|e_gw1.6.11.1|Name=jgi.p|Trimin1|158516|organism=Tribonema minus UTEX_B_3156 |type=polypeptide|length=113bp
MLFCPTCGNLLMVEAATRGGQRFACQTCPYVHPLGQGGKVTKALTLQRKE
VDDVLGGADAWVNVDKAPVACADCKDPGKGKEAYFFQAQLRSADEPMTVF
YKCVDCGFRWNE*
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mRNA from alignment at Contig_6:1394563..1395977-

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_2201 ID=mRNA_2201|Name=jgi.p|Trimin1|158516|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=1415bp|location=Sequence derived from alignment at Contig_6:1394563..1395977- (Tribonema minus UTEX_B_3156 )
ATGCTCTTCTGCCCCACGTGCGGCAACCTGCTCATGGTGGAGGCGGCGAC
GCGCGGCGGGCAGCGCTTTGCGTGCCAGACGTGCCCGTACGTGCACCCGC
TCGGGCAGGGCGGCAAGGTGACCAAGGCGCTCACGCTGCAGCGCAAGGAG
GTGGACGACGTGCTCGGCGGCGCGGACGCGTGGGTCAACGTCGACAAGGC
GCCCGTGGCGTGCGCCGACTGCAAGTGAGTGGACGCTCCGCGCGCGCCGC
ACGAGCCCCTTCCTCACTCTTTCGTAAAATCTTGCTTCAAAGTATTAGTT
TTTCCCATTCTTCAGAAGCCCAAAGAAATGGTTGACCGTCCTCTCAGACC
AACTCAAGATCACAGGCTTGTGTCGCCACGGCGCGCGACTTCAATACTTG
AAGTGTTTCACAGGCGAGGGCAGGTGCCGACAGCGTGGGAGTGGGCGCCG
TCCTTGCGTGCACGCGTGCGGAAGCGCCGTGTTTGCTGCGGACAGCGCAT
GCTCGCCGCACTGCGCTGCTGACCTCATTCTGTGACAATTGCTGGTACTT
ATTTGCGAGCAAGTATGTCCCAGAAACGAGGGTCAGATGCCAACTGCAAG
TGAGTGAACGCTCCACGCACGCACAGGGGTGCGTGCATGCGGCGCTCGCG
ACCAGCGCAAGCGAGCTACAGCGCACCAACGAGCCCATTAGTTGTTGTGT
ATGCCTAATTATCGCACACAGTAAGCCAGAAGTCTGATGCGGTTGTGAGT
TCGTTGTCAGACCCTTTCACATACTTTTGGATGGGAAGGGGGGGGGGGTG
GGGTAGCTGGCGCGTGTTGCTTGGTGAGTTGGCTGTGCGCATAGCACTGC
CACTGGGCGCTCTGTGCGTCCACAGTGTGCCGAGCGCCACAGTCAGTTGC
TAGTGTCTGATGCAATACCCAGTGCGCCGACCATCGAAGTTCACCGCCAA
GCATTTGTGATGTACTTCAACTGCAATGCAGACTTTTCGCGCAAGTTGCC
GATGCTCAGGGATTGAGTTCAGTAGTAGTAGCAGGAAGTCAAATTGTCGC
ACAGTGACCTTGCATGCCTGCTCAACAGTAGGTGGTTCAGAGCCTGCAGC
CGGCCACAGATCTGAAGTCACACGCAGAGTTTCTGCTGATGAAAAAAGGC
TTGTTTGTCCGCATTTTGAGCTCCGCATATCATCGGGCCACCATCAGTAC
AACACGTACCTGACCTGCATAAGCCACACATTGACTCCATTGCTGTCTAC
GTCATTCGAGAGCCAGCTGACTCCTGATCTGTTTTTCTGCGGCATGTCAG
GGATCCGGGCAAGGGCAAGGAGGCCTACTTCTTCCAGGCGCAGCTCAGAT
CCGCTGACGAGCCCATGACCGTGTTCTACAAATGCGTCGACTGTGGCTTC
CGCTGGAATGAATGA
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Coding sequence (CDS) from alignment at Contig_6:1394563..1395977-

>mRNA_2201 ID=mRNA_2201|Name=jgi.p|Trimin1|158516|organism=Tribonema minus UTEX_B_3156 |type=CDS|length=339bp|location=Sequence derived from alignment at Contig_6:1394563..1395977- (Tribonema minus UTEX_B_3156 )
ATGCTCTTCTGCCCCACGTGCGGCAACCTGCTCATGGTGGAGGCGGCGAC
GCGCGGCGGGCAGCGCTTTGCGTGCCAGACGTGCCCGTACGTGCACCCGC
TCGGGCAGGGCGGCAAGGTGACCAAGGCGCTCACGCTGCAGCGCAAGGAG
GTGGACGACGTGCTCGGCGGCGCGGACGCGTGGGTCAACGTCGACAAGGC
GCCCGTGGCGTGCGCCGACTGCAAGGATCCGGGCAAGGGCAAGGAGGCCT
ACTTCTTCCAGGCGCAGCTCAGATCCGCTGACGAGCCCATGACCGTGTTC
TACAAATGCGTCGACTGTGGCTTCCGCTGGAATGAATGA
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