mRNA_8569 (mRNA) Tribonema minus UTEX_B_3156

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
Namejgi.p|Trimin1|350128
Unique NamemRNA_8569
TypemRNA
OrganismTribonema minus UTEX_B_3156 (Tribonema minus UTEX_B_3156 )
Homology
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BLAST of jgi.p|Trimin1|350128 vs. uniprot
Match: A0A836CB06_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CB06_9STRA)

HSP 1 Score: 745 bits (1924), Expect = 3.770e-270
Identity = 409/409 (100.00%), Postives = 409/409 (100.00%), Query Frame = 1 
Query:   22 MESFMSLPPTGDGRTSRKRQQMSATMSAAVAEGEAPDPSHAPLQEIVLEQWLSRKAQRLDVAAAATALTVTQASALGGYGLAGRRPRGRPLAMRCKRKPRRRAVXXXXXXXXXXDTATRSXXXXXXXXADTAAIDAALQQADAHRACISANLAAAGSDGTITLVPHNSKDGQGRIAYFLTRAEARLYEGVNPMQLLLDAHHGGYAIATCSAVRSDGSCSPEVVLDLRLELRRRGGGGSGTDFVCTRDGLETRGSGAVVGRTAGSKAVKAARRDAGEPVERCTAYDHLSTDELVAQAALRCAYTVEGVPVAAAQQHVRAELDARLLPDGRSRLVPGKLKVAVACLMDEAVASAAHAAQDWVMLPGNVAWCPRAGCTRQRLPLWLLVAESRMEEWSAPAGYFLQTSCDGKL 1248
            MESFMSLPPTGDGRTSRKRQQMSATMSAAVAEGEAPDPSHAPLQEIVLEQWLSRKAQRLDVAAAATALTVTQASALGGYGLAGRRPRGRPLAMRCKRKPRRRAVXXXXXXXXXXDTATRSXXXXXXXXADTAAIDAALQQADAHRACISANLAAAGSDGTITLVPHNSKDGQGRIAYFLTRAEARLYEGVNPMQLLLDAHHGGYAIATCSAVRSDGSCSPEVVLDLRLELRRRGGGGSGTDFVCTRDGLETRGSGAVVGRTAGSKAVKAARRDAGEPVERCTAYDHLSTDELVAQAALRCAYTVEGVPVAAAQQHVRAELDARLLPDGRSRLVPGKLKVAVACLMDEAVASAAHAAQDWVMLPGNVAWCPRAGCTRQRLPLWLLVAESRMEEWSAPAGYFLQTSCDGKL
Sbjct:    1 MESFMSLPPTGDGRTSRKRQQMSATMSAAVAEGEAPDPSHAPLQEIVLEQWLSRKAQRLDVAAAATALTVTQASALGGYGLAGRRPRGRPLAMRCKRKPRRRAVXXXXXXXXXXDTATRSXXXXXXXXADTAAIDAALQQADAHRACISANLAAAGSDGTITLVPHNSKDGQGRIAYFLTRAEARLYEGVNPMQLLLDAHHGGYAIATCSAVRSDGSCSPEVVLDLRLELRRRGGGGSGTDFVCTRDGLETRGSGAVVGRTAGSKAVKAARRDAGEPVERCTAYDHLSTDELVAQAALRCAYTVEGVPVAAAQQHVRAELDARLLPDGRSRLVPGKLKVAVACLMDEAVASAAHAAQDWVMLPGNVAWCPRAGCTRQRLPLWLLVAESRMEEWSAPAGYFLQTSCDGKL 409
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BLAST of jgi.p|Trimin1|350128 vs. uniprot
Match: A0A836CKF5_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CKF5_9STRA)

HSP 1 Score: 344 bits (882), Expect = 1.210e-107
Identity = 203/347 (58.50%), Postives = 227/347 (65.42%), Query Frame = 1 
Query:  409 DTAAIDAALQQADAHRACISANLAAAGSDGTITLVPHNSKDGQGRIAYFLTRAEA---------------------------RLYEGVNPMQLLLDAHHGGYAIATCSAVRSDGSCSPEVVLDLRLELRRRGGGGSGTDFVCT----------------------------------------RDGLETRGSGAVVGRTAGSKAVKAARRDAGEPVERCTAYDHLSTDELVAQAALRCAYTVEGVPVAAAQQHVRAELDARLLPDGRSRLVPGKLKVAVACLMDEAVASAAHAAQDWVMLPGNVAWCPRAGCTRQRLPLWLLVAESRMEEWSAPAGYFLQTSCDGKL 1248
            D   I  A QQAD HRA ISA LAAAG D T+TLVPH +K+G GRIAYFL RAEA                           +LY+GV+P+++LLDA  GGYAIAT SA+R DGSCSPEVVL LRLEL+RRGGGGSGT+FVCT                                        RDGL+TRGSGAVVGRTAGSKAVKA RRDA EPVER TAY  LSTD+LV +AALRCAYTV+G  VAAAQQ VR EL+ARLLPD   R+ P KLKV  A L DEA+A AAH  QDWV+LPG V WCPRAGCTR+  P  LLVAESR+ E SAPAGY L+TS  G +
Sbjct:   81 DARGISDASQQADVHRAIISAKLAAAGPDDTVTLVPHYTKEGAGRIAYFLKRAEAIEFIRKNQDSFGGVPPCMDTVRAAALGQLYDGVDPLKVLLDARQGGYAIATRSALRGDGSCSPEVVLGLRLELQRRGGGGSGTEFVCTWAEIQRFQQFGDGPQSQCVFGPIEEVLCGEPGATVPLHRIRDGLQTRGSGAVVGRTAGSKAVKATRRDAAEPVERSTAYGDLSTDQLVTKAALRCAYTVKGTTVAAAQQRVRVELEARLLPDDDGRITPRKLKVGFARLEDEALAIAAHTEQDWVVLPGGVEWCPRAGCTRRWRPRRLLVAESRVGELSAPAGYTLETSRGGDV 427
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BLAST of jgi.p|Trimin1|350128 vs. uniprot
Match: A0A835YI09_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YI09_9STRA)

HSP 1 Score: 161 bits (407), Expect = 1.680e-44
Identity = 91/120 (75.83%), Postives = 98/120 (81.67%), Query Frame = 1 
Query:  409 DTAAIDAALQQADAHRACISANL--AAAGSDGTITLVPHNSKDGQGRIAYFLTRAEARLYEGVNPMQ---LLLDAHHGGYAIATCSAVRSDGSCSPEVVLDLRLELRRRGGGGSGTDFVC 753
            DTAAI AALQQAD +RACISANL  AAAG DGTITLV HN+ +G+GRIAYFLTRA+A  +   N      LLLDA  GGYAIA C A+RSDGSCSPEVVLDLRLELRRRGGGGSGTDFVC
Sbjct:    6 DTAAITAALQQADENRACISANLQDAAAGPDGTITLVAHNNLEGEGRIAYFLTRAKAMEFIRKNHGTFNGLLLDARQGGYAIAACRALRSDGSCSPEVVLDLRLELRRRGGGGSGTDFVC 125
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BLAST of jgi.p|Trimin1|350128 vs. uniprot
Match: A0A835ZEI0_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZEI0_9STRA)

HSP 1 Score: 95.9 bits (237), Expect = 1.020e-17
Identity = 55/84 (65.48%), Postives = 60/84 (71.43%), Query Frame = -3 
Query:  994 LAVAAGLQEVACRR*PLLHPALRHEQPERQPLTRAAGTWAPCHVARQHHPVLRRVGRAGNRLVHQARDCHLQLAGHKAAAAVRQ 1245
            +A AA LQ VA RR  L  PALRH+QP RQPLTRAAG WAP HVARQ HP+L RV R G+RLV Q RD HL+ A   A  AVRQ
Sbjct:  326 VAAAAALQGVAGRRRRLPGPALRHQQPVRQPLTRAAGMWAPLHVARQCHPLLLRVCRYGDRLVLQPRDAHLEFASCNADVAVRQ 409
The following BLAST results are available for this feature:
BLAST of jgi.p|Trimin1|350128 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 4
Match NameE-valueIdentityDescription
A0A836CB06_9STRA3.770e-270100.00Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A836CKF5_9STRA1.210e-10758.50Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A835YI09_9STRA1.680e-4475.83Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A835ZEI0_9STRA1.020e-1765.48Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Contig_57contigContig_57:113940..116224 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Tribonema minus UTEX_B_31562021-06-17
Properties
Property NameValue
ProteomeIdTrimin1|350128|estExt_Genemark1.C_Ctg_570020
Hectar predicted targeting categoryother localisation
TranscriptId350450
TrackFilteredModels1
ProteinId350128
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameSpeciesTypePosition
jgi.p|Trimin1|350128gene_8569Tribonema minus UTEX_B_3156 geneContig_57 113940..116224 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
jgi.p|Trimin1|350128Trimin1|350128|estExt_Genemark1.C_Ctg_570020Tribonema minus UTEX_B_3156 polypeptideContig_57 113961..116159 +


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
exon_8569_1exon_8569_1Tribonema minus UTEX_B_3156 exonContig_57 113940..114226 +
exon_8569_2exon_8569_2Tribonema minus UTEX_B_3156 exonContig_57 114884..114923 +
exon_8569_3exon_8569_3Tribonema minus UTEX_B_3156 exonContig_57 115031..115276 +
exon_8569_4exon_8569_4Tribonema minus UTEX_B_3156 exonContig_57 115362..115543 +
exon_8569_5exon_8569_5Tribonema minus UTEX_B_3156 exonContig_57 115664..116224 +


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
UTR5_8569UTR5_8569Tribonema minus UTEX_B_3156 five_prime_UTRContig_57 113940..113960 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
CDS_8569CDS_8569Tribonema minus UTEX_B_3156 CDSContig_57 113961..114226 +


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
UTR3_8569UTR3_8569Tribonema minus UTEX_B_3156 three_prime_UTRContig_57 116160..116224 +


Sequences
The following sequences are available for this feature:

spliced messenger RNA

>mRNA_8569 ID=mRNA_8569|Name=jgi.p|Trimin1|350128|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=1316bp|location=Sequence derived from alignment at Contig_57:113940..116224+ (Tribonema minus UTEX_B_3156 )|Notes=Excludes all bases but those of type(s): exon.  
GAGAGAGAGAGAGGGTCAGTTATGGAGTCGTTCATGTCGTTGCCGCCCAC
AGGGGATGGGCGTACGAGCCGGAAGCGGCAACAAATGTCAGCGACTATGT
CGGCTGCAGTGGCAGAGGGGGAGGCGCCGGATCCCAGCCACGCGCCACTG
CAGGAAATCGTGCTGGAGCAATGGCTTTCCCGCAAAGCGCAGCGGCTGGA
CGTCGCGGCCGCTGCGACTGCCCTGACAGTCACACAGGCATCCGCCCTCG
GAGGCTATGGCCTTGCAGGGCGCAGACCGAGGGGCAGGCCGCTCGCTATG
CGTTGCAAGCGCAAGCCGCGGCGGCGGGCAGTGGCCGCTGTCGCCGCCGC
CGCCGCCCGTGCCGACACCGCCACCAGATCCGCCGCCGTCGCCGTTGCCG
CCGCCGCCGACACCGCCGCCATCGATGCCGCACTTCAGCAAGCCGACGCA
CACCGCGCCTGCATTTCCGCCAACCTTGCCGCCGCAGGGTCGGACGGCAC
CATCACACTGGTGCCGCACAACAGCAAGGACGGTCAAGGGCGCATCGCGT
ACTTTTTGACGCGAGCGGAGGCGCGGCTTTACGAGGGCGTCAACCCCATG
CAGCTCCTGCTCGACGCCCACCACGGCGGCTACGCAATCGCCACGTGCAG
CGCCGTGCGTAGCGACGGGAGCTGCAGCCCGGAGGTAGTTCTGGACCTTC
GTTTGGAGCTGCGGCGGCGCGGCGGCGGCGGCAGCGGTACCGACTTTGTG
TGCACACGCGACGGCCTGGAGACGAGGGGATCTGGCGCCGTCGTGGGCCG
CACAGCTGGATCCAAGGCCGTCAAGGCCGCGCGGCGCGATGCAGGCGAGC
CCGTTGAGCGCTGCACGGCGTACGACCACTTAAGCACCGATGAACTCGTG
GCGCAGGCGGCACTGCGCTGCGCATACACGGTGGAAGGCGTACCGGTGGC
GGCAGCGCAGCAGCATGTGCGGGCGGAGCTGGACGCGCGGCTGCTGCCTG
ACGGCCGCAGCCGCCTTGTGCCCGGCAAGCTGAAGGTGGCAGTCGCGTGC
CTGATGGACGAGGCGGTTGCCAGCGCGGCCCACGCGGCGCAGGACTGGGT
GATGCTGCCTGGCAACGTGGCATGGTGCCCACGTGCCGGCTGCACGCGTC
AGCGGCTGCCGCTCTGGCTGCTCGTGGCGGAGAGCAGGATGGAGGAGTGG
TCAGCGCCGGCAGGCTACTTCCTGCAGACCAGCTGCGACGGCAAGCTATG
AGGCTCTCCTGCACTTGAGCAGCCGCGCGCGCGCATGAAGTCATGATAAC
AAATTCATGATGCTCA
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protein sequence of jgi.p|Trimin1|350128

>Trimin1|350128|estExt_Genemark1.C_Ctg_570020 ID=Trimin1|350128|estExt_Genemark1.C_Ctg_570020|Name=jgi.p|Trimin1|350128|organism=Tribonema minus UTEX_B_3156 |type=polypeptide|length=410bp
MESFMSLPPTGDGRTSRKRQQMSATMSAAVAEGEAPDPSHAPLQEIVLEQ
WLSRKAQRLDVAAAATALTVTQASALGGYGLAGRRPRGRPLAMRCKRKPR
RRAVAAVAAAAARADTATRSAAVAVAAAADTAAIDAALQQADAHRACISA
NLAAAGSDGTITLVPHNSKDGQGRIAYFLTRAEARLYEGVNPMQLLLDAH
HGGYAIATCSAVRSDGSCSPEVVLDLRLELRRRGGGGSGTDFVCTRDGLE
TRGSGAVVGRTAGSKAVKAARRDAGEPVERCTAYDHLSTDELVAQAALRC
AYTVEGVPVAAAQQHVRAELDARLLPDGRSRLVPGKLKVAVACLMDEAVA
SAAHAAQDWVMLPGNVAWCPRAGCTRQRLPLWLLVAESRMEEWSAPAGYF
LQTSCDGKL*
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mRNA from alignment at Contig_57:113940..116224+

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_8569 ID=mRNA_8569|Name=jgi.p|Trimin1|350128|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=2285bp|location=Sequence derived from alignment at Contig_57:113940..116224+ (Tribonema minus UTEX_B_3156 )
GAGAGAGAGAGAGGGTCAGTTATGGAGTCGTTCATGTCGTTGCCGCCCAC
AGGGGATGGGCGTACGAGCCGGAAGCGGCAACAAATGTCAGCGACTATGT
CGGCTGCAGTGGCAGAGGGGGAGGCGCCGGATCCCAGCCACGCGCCACTG
CAGGAAATCGTGCTGGAGCAATGGCTTTCCCGCAAAGCGCAGCGGCTGGA
CGTCGCGGCCGCTGCGACTGCCCTGACAGTCACACAGGCATCCGCCCTCG
GAGGCTATGGCCTTGCAGGGCGCAGACCGAGGGGCAGGTGAGCAAATCGA
TTCGGATCTTGCACTGCAGATAGCGGAGCATCCCACATGATGGCGTCGGC
TGCATGTTTGCTCATGCAATGCCCAAATGCAGCTTGAGTGCTCATGCAAA
GCCCAAATGCTGCTGTTGATGACTTGTTGGGCATCTGCAGTCTGCGGGTG
CACGATATACACATGATCATCAGATCTTTTGCACATGAAGGCCAAAACGT
GCATTCAATGTCTTGAACATCGATGGCACAGAGGATCATCGACACTGAAC
CACCGTGTACCGGCCTGTTCTGAACTGTCACTAACAAATGCCTGCCGCTT
GCCCTGCACTACCTTGGACTGGAAGTGCATGCAGTTTGCAAAATGCACTG
TGCAGCACTGGCAGGACGCCGCCGTTGATTTGAGTCATGTCAAACACTGC
TTACAATGCAGGCATCAACTGGAACAGCGGCTTCCCGACAAAAGATTGCA
AAGATCCATATCAAAAGCTTCTGGTGGATAGCTCTTCATAAAATGAACTG
TGGTTCACAGCGCCCACAACAGTAAATGTTGTAAATTACTCATTTACACA
CGTCGGATCATCATGCGATCTTTGCAAAAGATTTGGCAAAACGCTATTCC
TGCTCTCTCGCTCCGCTGACACATTCTGCCTTGTGAATGTGCAGGCCGCT
CGCTATGCGTTGCAAGCGCAAGCCGCGGCGGCGGGTGCTGAGCCACCACA
TGCGCCGTCGGAGACCTCTGCCGCCGCCGCAGCACCCGCACCACCCGCAC
CACCAACCGCACCAGCCTGCGATCACCCTCGCCGCGCCAAGGCAGTGGCC
GCTGTCGCCGCCGCCGCCGCCCGTGCCGACACCGCCACCAGATCCGCCGC
CGTCGCCGTTGCCGCCGCCGCCGACACCGCCGCCATCGATGCCGCACTTC
AGCAAGCCGACGCACACCGCGCCTGCATTTCCGCCAACCTTGCCGCCGCA
GGGTCGGACGGCACCATCACACTGGTGCCGCACAACAGCAAGGACGGTCA
AGGGCGCATCGCGTACTTTTTGACGCGAGCGGAGGCGGTCGGTCGCCTTC
ATCACGAACAACCACGGCACCTTCGGCGGCGTCCCGCCCTGTCTGGACAC
CGTCCGCGCAGCGGCACTCCAGCGGCTTTACGAGGGCGTCAACCCCATGC
AGCTCCTGCTCGACGCCCACCACGGCGGCTACGCAATCGCCACGTGCAGC
GCCGTGCGTAGCGACGGGAGCTGCAGCCCGGAGGTAGTTCTGGACCTTCG
TTTGGAGCTGCGGCGGCGCGGCGGCGGCGGCAGCGGTACCGACTTTGTGT
GCACGTGGGCTCAAGTGCAACGCTACGAGCAGACCGGCGATGGGCCGCAA
AGCCAATGCGTCTTCGGCCCCATCGACTAGGTACTCTGCGGCGAGCCTGG
CGTCGCGGTGCCGTTGCACCGCAGACGCGACGGCCTGGAGACGAGGGGAT
CTGGCGCCGTCGTGGGCCGCACAGCTGGATCCAAGGCCGTCAAGGCCGCG
CGGCGCGATGCAGGCGAGCCCGTTGAGCGCTGCACGGCGTACGACCACTT
AAGCACCGATGAACTCGTGGCGCAGGCGGCACTGCGCTGCGCATACACGG
TGGAAGGCGTACCGGTGGCGGCAGCGCAGCAGCATGTGCGGGCGGAGCTG
GACGCGCGGCTGCTGCCTGACGGCCGCAGCCGCCTTGTGCCCGGCAAGCT
GAAGGTGGCAGTCGCGTGCCTGATGGACGAGGCGGTTGCCAGCGCGGCCC
ACGCGGCGCAGGACTGGGTGATGCTGCCTGGCAACGTGGCATGGTGCCCA
CGTGCCGGCTGCACGCGTCAGCGGCTGCCGCTCTGGCTGCTCGTGGCGGA
GAGCAGGATGGAGGAGTGGTCAGCGCCGGCAGGCTACTTCCTGCAGACCA
GCTGCGACGGCAAGCTATGAGGCTCTCCTGCACTTGAGCAGCCGCGCGCG
CGCATGAAGTCATGATAACAAATTCATGATGCTCA
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Coding sequence (CDS) from alignment at Contig_57:113940..116224+

>mRNA_8569 ID=mRNA_8569|Name=jgi.p|Trimin1|350128|organism=Tribonema minus UTEX_B_3156 |type=CDS|length=1230bp|location=Sequence derived from alignment at Contig_57:113940..116224+ (Tribonema minus UTEX_B_3156 )
ATGGAGTCGTTCATGTCGTTGCCGCCCACAGGGGATGGGCGTACGAGCCG
GAAGCGGCAACAAATGTCAGCGACTATGTCGGCTGCAGTGGCAGAGGGGG
AGGCGCCGGATCCCAGCCACGCGCCACTGCAGGAAATCGTGCTGGAGCAA
TGGCTTTCCCGCAAAGCGCAGCGGCTGGACGTCGCGGCCGCTGCGACTGC
CCTGACAGTCACACAGGCATCCGCCCTCGGAGGCTATGGCCTTGCAGGGC
GCAGACCGAGGGGCAGGCCGCTCGCTATGCGTTGCAAGCGCAAGCCGCGG
CGGCGGGCAGTGGCCGCTGTCGCCGCCGCCGCCGCCCGTGCCGACACCGC
CACCAGATCCGCCGCCGTCGCCGTTGCCGCCGCCGCCGACACCGCCGCCA
TCGATGCCGCACTTCAGCAAGCCGACGCACACCGCGCCTGCATTTCCGCC
AACCTTGCCGCCGCAGGGTCGGACGGCACCATCACACTGGTGCCGCACAA
CAGCAAGGACGGTCAAGGGCGCATCGCGTACTTTTTGACGCGAGCGGAGG
CGCGGCTTTACGAGGGCGTCAACCCCATGCAGCTCCTGCTCGACGCCCAC
CACGGCGGCTACGCAATCGCCACGTGCAGCGCCGTGCGTAGCGACGGGAG
CTGCAGCCCGGAGGTAGTTCTGGACCTTCGTTTGGAGCTGCGGCGGCGCG
GCGGCGGCGGCAGCGGTACCGACTTTGTGTGCACACGCGACGGCCTGGAG
ACGAGGGGATCTGGCGCCGTCGTGGGCCGCACAGCTGGATCCAAGGCCGT
CAAGGCCGCGCGGCGCGATGCAGGCGAGCCCGTTGAGCGCTGCACGGCGT
ACGACCACTTAAGCACCGATGAACTCGTGGCGCAGGCGGCACTGCGCTGC
GCATACACGGTGGAAGGCGTACCGGTGGCGGCAGCGCAGCAGCATGTGCG
GGCGGAGCTGGACGCGCGGCTGCTGCCTGACGGCCGCAGCCGCCTTGTGC
CCGGCAAGCTGAAGGTGGCAGTCGCGTGCCTGATGGACGAGGCGGTTGCC
AGCGCGGCCCACGCGGCGCAGGACTGGGTGATGCTGCCTGGCAACGTGGC
ATGGTGCCCACGTGCCGGCTGCACGCGTCAGCGGCTGCCGCTCTGGCTGC
TCGTGGCGGAGAGCAGGATGGAGGAGTGGTCAGCGCCGGCAGGCTACTTC
CTGCAGACCAGCTGCGACGGCAAGCTATGA
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