mRNA_5460 (mRNA) Tribonema minus UTEX_B_3156

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
Namejgi.p|Trimin1|147988
Unique NamemRNA_5460
TypemRNA
OrganismTribonema minus UTEX_B_3156 (Tribonema minus UTEX_B_3156 )
Homology
BLAST of jgi.p|Trimin1|147988 vs. uniprot
Match: A0A835Z3C8_9STRA (Uncharacterized protein (Fragment) n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z3C8_9STRA)

HSP 1 Score: 677 bits (1746), Expect = 2.040e-245
Identity = 353/353 (100.00%), Postives = 353/353 (100.00%), Query Frame = 1
Query:    1 EFGAQDFLEPGRQFVLEGDLIKITRGGRKEHKFVLFNDKLVYGEEKGLLMRRTGMFANVVSGNTKRQYRKAYDFPLSRGLYVVNDVGGAYASARSGXXXXXAAEGVLGDTGFMFITPDKSFVVHAASPAEKDAWVQALRRVMADLTAKGDSRALKVDMDLSDKVKPAKSGKSISCDIFVWVIRDGKTMRQGFSLPPSALSAPFGVLSYSELVQRGAQVDPSPTRAGLPDDDLGFGLEWDAPPALQLEVLPDPFDDDYDDPLGGSRLQVEVSLQPGDRLGLRLRDIQGGGVLVMGFITRGCAAQVAGVCQGDRVTHIDGTGPLLSAAEAXXXXXXXLDRCGGGGVLKLTLRRGF 1059
            EFGAQDFLEPGRQFVLEGDLIKITRGGRKEHKFVLFNDKLVYGEEKGLLMRRTGMFANVVSGNTKRQYRKAYDFPLSRGLYVVNDVGGAYASARSGXXXXXAAEGVLGDTGFMFITPDKSFVVHAASPAEKDAWVQALRRVMADLTAKGDSRALKVDMDLSDKVKPAKSGKSISCDIFVWVIRDGKTMRQGFSLPPSALSAPFGVLSYSELVQRGAQVDPSPTRAGLPDDDLGFGLEWDAPPALQLEVLPDPFDDDYDDPLGGSRLQVEVSLQPGDRLGLRLRDIQGGGVLVMGFITRGCAAQVAGVCQGDRVTHIDGTGPLLSAAEAXXXXXXXLDRCGGGGVLKLTLRRGF
Sbjct:    1 EFGAQDFLEPGRQFVLEGDLIKITRGGRKEHKFVLFNDKLVYGEEKGLLMRRTGMFANVVSGNTKRQYRKAYDFPLSRGLYVVNDVGGAYASARSGXXXXXAAEGVLGDTGFMFITPDKSFVVHAASPAEKDAWVQALRRVMADLTAKGDSRALKVDMDLSDKVKPAKSGKSISCDIFVWVIRDGKTMRQGFSLPPSALSAPFGVLSYSELVQRGAQVDPSPTRAGLPDDDLGFGLEWDAPPALQLEVLPDPFDDDYDDPLGGSRLQVEVSLQPGDRLGLRLRDIQGGGVLVMGFITRGCAAQVAGVCQGDRVTHIDGTGPLLSAAEAXXXXXXXLDRCGGGGVLKLTLRRGF 353          
BLAST of jgi.p|Trimin1|147988 vs. uniprot
Match: A0A1E1XGQ0_9ACAR (Putative rho/rac guanine nucleotide exchange factor/faciogenital dysplasia protein 3 n=1 Tax=Amblyomma aureolatum TaxID=187763 RepID=A0A1E1XGQ0_9ACAR)

HSP 1 Score: 55.8 bits (133), Expect = 9.020e-5
Identity = 41/139 (29.50%), Postives = 64/139 (46.04%), Query Frame = 1
Query:   16 DFLEPGRQFVLEGDLIKITRGGRKEHKFVLFNDKLVYGEEKGLLMRRTGMFANVVSGNTKRQYRKAYDFPLSRGLYVVNDVGGAYASARSGXXXXXAAEGVLGDTGFMFITPDKSFVVHAASPAEKDAWVQALRRVMAD 432
            + L+PGR F+ EG+L+K++R   +   FVLF+D L+Y     LL    G             YR  Y+ PLS G+ V+      Y +                   F   T  +SF++ A+SP E++ W+ AL + + D
Sbjct: 1343 EVLKPGRTFLKEGELMKVSRKEMQPRYFVLFSDSLLY-----LLPTPQGF------------YRVNYELPLS-GMKVMVPPQQDYQNE------------------FSVYTMKRSFILSASSPEEREEWISALTKAIED 1445          
The following BLAST results are available for this feature:
BLAST of jgi.p|Trimin1|147988 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 2
Match NameE-valueIdentityDescription
A0A835Z3C8_9STRA2.040e-245100.00Uncharacterized protein (Fragment) n=1 Tax=Tribone... [more]
A0A1E1XGQ0_9ACAR9.020e-529.50Putative rho/rac guanine nucleotide exchange facto... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Contig_19contigContig_19:96491..101227 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Tribonema minus UTEX_B_31562021-06-17
Properties
Property NameValue
ProteomeIdTrimin1|147988|gw1.19.143.1
DescriptionRho guanyl-nucleotide exchange factor activity
COG categoryT
Max annot lvl2759|Eukaryota
EggNOG OGsCOG5422@1|root,KOG4424@2759|Eukaryota
Evalue3.62e-22
Seed ortholog2880.D8LKY8
Hectar predicted targeting categoryother localisation
TranscriptId148310
TrackFilteredModels1
ProteinId147988
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameSpeciesTypePosition
jgi.p|Trimin1|147988gene_5460Tribonema minus UTEX_B_3156 geneContig_19 96491..101227 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
jgi.p|Trimin1|147988Trimin1|147988|gw1.19.143.1Tribonema minus UTEX_B_3156 polypeptideContig_19 96491..101227 +


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
exon_5460_1exon_5460_1Tribonema minus UTEX_B_3156 exonContig_19 96491..96556 +
exon_5460_2exon_5460_2Tribonema minus UTEX_B_3156 exonContig_19 97594..97935 +
exon_5460_3exon_5460_3Tribonema minus UTEX_B_3156 exonContig_19 98886..98966 +
exon_5460_4exon_5460_4Tribonema minus UTEX_B_3156 exonContig_19 99631..99882 +
exon_5460_5exon_5460_5Tribonema minus UTEX_B_3156 exonContig_19 100240..100407 +
exon_5460_6exon_5460_6Tribonema minus UTEX_B_3156 exonContig_19 101078..101227 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
CDS_5460CDS_5460Tribonema minus UTEX_B_3156 CDSContig_19 96491..96556 +


Sequences
The following sequences are available for this feature:

spliced messenger RNA

>mRNA_5460 ID=mRNA_5460|Name=jgi.p|Trimin1|147988|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=1059bp|location=Sequence derived from alignment at Contig_19:96491..101227+ (Tribonema minus UTEX_B_3156 )|Notes=Excludes all bases but those of type(s): exon.  
GAGTTTGGTGCGCAAGACTTCCTGGAGCCCGGCCGGCAGTTTGTGCTGGA
GGGCGACCTTATTAAGATCACACGCGGCGGCCGCAAAGAGCACAAGTTCG
TGCTCTTCAATGACAAGCTCGTCTATGGCGAAGAGAAAGGCCTACTCATG
CGCCGCACGGGCATGTTTGCCAACGTCGTCAGCGGCAACACCAAGCGCCA
GTACCGCAAGGCCTACGACTTCCCCCTGAGCAGGGGGCTGTACGTGGTCA
ACGACGTGGGGGGTGCGTATGCCAGCGCGCGTAGCGGGGGAGGGGGCGGG
GGGGCAGCGGAGGGGGTTTTGGGGGACACGGGCTTCATGTTCATCACGCC
GGATAAGAGTTTTGTCGTGCACGCTGCGTCGCCTGCGGAGAAGGACGCGT
GGGTGCAGGCATTACGACGCGTGATGGCTGACCTGACGGCCAAGGGTGAC
TCGCGCGCGCTCAAGGTCGACATGGATCTGTCAGACAAGGTCAAGCCCGC
CAAAAGCGGCAAATCCATAAGCTGCGACATCTTCGTCTGGGTGATTCGCG
ACGGGAAGACCATGCGCCAGGGGTTCTCCCTCCCCCCGTCCGCCCTGTCC
GCCCCCTTTGGCGTCCTCTCGTACAGCGAGCTGGTCCAGAGGGGGGCGCA
AGTGGACCCCTCCCCCACCCGCGCTGGCCTTCCTGATGACGATCTAGGGT
TCGGTCTGGAGTGGGATGCGCCCCCCGCACTTCAACTTGAGGTGCTACCG
GACCCCTTCGACGATGACTACGATGACCCCCTCGGGGGGTCACGACTTCA
AGTTGAAGTTTCCCTTCAACCGGGCGACCGGCTGGGACTGCGCCTGCGGG
ATATACAGGGGGGCGGCGTGCTGGTGATGGGGTTCATCACCAGGGGGTGC
GCTGCGCAGGTTGCTGGCGTCTGCCAAGGCGACCGAGTCACCCATATCGA
CGGCACTGGACCGCTATTATCAGCAGCAGAGGCGGCGGCCGCAGCAGCAG
CAGCGCTCGACCGCTGCGGCGGTGGGGGAGTATTGAAGTTGACGCTCCGC
AGGGGGTTC
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protein sequence of jgi.p|Trimin1|147988

>Trimin1|147988|gw1.19.143.1 ID=Trimin1|147988|gw1.19.143.1|Name=jgi.p|Trimin1|147988|organism=Tribonema minus UTEX_B_3156 |type=polypeptide|length=353bp
EFGAQDFLEPGRQFVLEGDLIKITRGGRKEHKFVLFNDKLVYGEEKGLLM
RRTGMFANVVSGNTKRQYRKAYDFPLSRGLYVVNDVGGAYASARSGGGGG
GAAEGVLGDTGFMFITPDKSFVVHAASPAEKDAWVQALRRVMADLTAKGD
SRALKVDMDLSDKVKPAKSGKSISCDIFVWVIRDGKTMRQGFSLPPSALS
APFGVLSYSELVQRGAQVDPSPTRAGLPDDDLGFGLEWDAPPALQLEVLP
DPFDDDYDDPLGGSRLQVEVSLQPGDRLGLRLRDIQGGGVLVMGFITRGC
AAQVAGVCQGDRVTHIDGTGPLLSAAEAAAAAAAALDRCGGGGVLKLTLR
RGF
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mRNA from alignment at Contig_19:96491..101227+

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_5460 ID=mRNA_5460|Name=jgi.p|Trimin1|147988|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=4737bp|location=Sequence derived from alignment at Contig_19:96491..101227+ (Tribonema minus UTEX_B_3156 )
GAGTTTGGTGCGCAAGACTTCCTGGAGCCCGGCCGGCAGTTTGTGCTGGA GGGCGACCTTATTAAGGTGAGTCAGGCACCACTTAAGGTTAGGGTTAGGA TTAGGGTTGGGTTAAAAGAACATGCCTTTGGTGCTGCAAAAAAGTGTGTG TGTGTGGCGGGGACATTTCGGCCACCTCAGGGTTACGCTTAGAATTAGGG TTAGAGGGTCTTCCACCAGGCCGTGTTCCTGCCAGGCCGGTGTGTGCGTG TGTGTGTGGGTGAACGGGGGCAAGAACGATGCCAGTCGACAGAATGAGGG GTCTTCCTTCTGCTTGTATGGGGTATGTATGCTTGTATGGGATACTCAAG GTCGTTCCAAAACTCAAGGTCGTTCCAAAACGGTCATTGCTCTGGTCCAT ATACCGCTAGACCTAGCAGTAGACGTAGGCGGTAGACCTAGAAGTACACG CTGCAGTGTGGGTGCACGGGCGTCTGCAGTGGTGCGCTTTGTGACATGCA CGTGCAGACAGTGCCGTGACGCAAACGCTAGCCCTAGCCCTGCGTCTTGC GCACACCTGCCCATTTGACTCCAGCCGCGTGCGCAGACGCTAGCCCTAAC CCTAGGTATGATGCATACCTACCCGTTACGCAGATCACGCGCGGCAGCCG CAAAGAGCACAACTTTGTCCGCGTTATCATGAATCTTCCTACTGGGAACT GGCATCGTACAACATGCAACGGCATTCACGCATCGGCATTTACACATTAA TGGCTGAGACACCCAGCAGGCATGTGCAGTAAGCGCCGTCCCCCCCCCCC CCAATCCCTCACTTCAACCCTCATCCTAGGTCCTGAGCGGTGACGCAAGC CCTACAGTACCTCAAACCTAACCCTACCTCCTGAGCCGTGACACAAAAGC GAGCCCCAACCCTAGGTCTGATGCAAACTTACCCACCTACCCTCCAACCA ATGCACGCGCAGATCACGCGCAGCGGCCGCAAGGAGCGCATGTTTGTGCT CTTCAGCGACGAAGCTGGTGTACAGCGAAGCGAAAGGGCTACCCTAACCC TAACCCTAGGTCTGATTTGTACGTACCTACATACCCCCCAATCTTGCATG CAGATCACACGCGGCGGCCGCAAAGAGCACAAGTTCGTGCTCTTCAATGA CAAGCTCGTCTATGGCGAAGAGAAAGGCCTACTCATGCGCCGCACGGGCA TGTTTGCCAACGTCGTCAGCGGCAACACCAAGCGCCAGTACCGCAAGGCC TACGACTTCCCCCTGAGCAGGGGGCTGTACGTGGTCAACGACGTGGGGGG TGCGTATGCCAGCGCGCGTAGCGGGGGAGGGGGCGGGGGGGCAGCGGAGG GGGTTTTGGGGGACACGGGCTTCATGTTCATCACGCCGGATAAGAGTTTT GTCGTGCACGCTGCGTCGCCTGCGGAGAAGGACGCGTGGGTGCAGGTGCG TCATGGCGGCAATTATGCAGTTGGCAGTTATGTGGCAGTTACCACGTGGC GTCGCCCATGCAGTTGCGTGGGTGCAGGTGCAGTTCGCATCCGGCAGTTA CATGGCAGTTGCACAATTTGTTTTCGATACTTCTATTAGAATGCGGGGCA AGCTTTGTCGCGCACGCGGCGTCACACGCGGAAAATTATGCGTGGGCAGT TACATGGCAGTTACACATTACGACGCCGGACAAGAGCTTTGTCTTGCATG CTGCGTCACCTGCTGAAAAGGATGCGCGGGTTCAGGCGCGTACCAACGGC TGTTACGCAAACAGCAGTTACATGGCAGTTGCACAGAATTCATGATCCAC GGCAGGAGCTGCGTGGTGCATGTGGCGTCGCCTGACCGGCGCGTGCGCGG GCGGCGTCAGTCGCGCCGTTGGCCGTCACAGAGCAGTTGCGCAGTCTGCA TGGTCAGGAGAGGTTTCGTGGTTCACGTGCTGTCGCTAGCGGAGAAGCTG AGAGGCACGAATGGCAGCTACACAAACGGCAGTTACGCAGTTTGCCACAC TGCTCAGCATCAGAAGGGTCTCTGTGCAACGCGGCTCGAAAACCCCTAGG GTTAGGCTTAGGGTTAGGGTTAGGATTAGGGTTAGGGTTAAGGTTAACCC TAACCCCAAGACATCCATCACTGTACTCGCAACTTCCAGTTCCATGGAGG ATGAAGAGGCTCACAAGCACGGCGCAAGTGAACAGTGAACATGCTGCTGT TGTTCAGCATGCAGCGCGCATTGCAGTTCTGCCTTGCTGCGCTCTTGAGG CTAAAGTAAGGGCTAACCTAACCCTAACACTAATGGAAACGAATCAAACG ATCGTAGAGCCGTAGGAGTTAAGGAGTGGTTAGCCGCAAGTGCCTTCCAC CAATGATCCTAACCCTAACCCTATCCCTCTTCTCAATGTGTGCAGGCATT ACGACGCGTGATGGCTGACCTGACGGCCAAGGGTGACTCGCGCGCGCTCA AGGTCGACATGGATCTGTCAGACAAGGTGCGCTTATTAGCAGGTCATCAT TGCTGGTGTGCCGTGCGCATCATCGATTCTGATTCAGGTGGCTATTAGTC TGTTTCTGAAATAGCCATTGATATATGCACAGGGTAGAGTCAGACACGGG CCATGAGTCGCTGAGATCAGCCACTGCGGCGCGTGCAATACAAACACTTT TCGACAACTCACAATCTTTGATTCGAAGAAACATTGATCTATCGGACAGC GTGGGACCGTTGCCATGCATGCAAAATCTGACCGGGCTGGCATTCAGGCT CCGCATTGACATTGACATCGCATTCGCGTTATGCTCACGTCAGCAGCTGG TGTGTCACACCACCCGCGCCTCTTGCTCAAGTGATGGCTGGGCATGAGAA ATCTGGGCACAATACCCTGCAAGTGCGCTCTTCCGGCGCGTAAACTCCCA AATAATTGACACCTGAACCCCCTCCCCCCCCTTCAACTCAGGTCAAGCCC ACCAAAAGCGGCAAGTCCATATTGCGCGGTTTCAACCCCCCCCTCCCCCC CCCTCCCCCCCTCCCCTTCCCTTCTCTTTTCATCTCAGGTCAAGCCCGCC AAAAGCGGCAAATCCACATTGCGCGCCCTCACCAACTTGCAATGAATTGA CCCCCCCCTCCCCTCCCCTTTTCTTCTCTTTTCAACTCAGGTCAAGCCCG CCAAAAGCGGCAAATCCATAAGCTGCGACATCTTCGTCTGGGTGATTCGC GACGGGAAGACCATGCGCCAGGGGTTCTCCCTCCCCCCGTCCGCCCTGTC CGCCCCCTTTGGCGTCCTCTCGTACAGCGAGCTGGTCCAGAGGGGGGCGC AAGTGGACCCCTCCCCCACCCGCGCTGGCCTTCCTGATGACGATCTAGGG TTCGGTCTGGAGTGGGATGCGCCCCCCGCACTTCAACTTGAGGTGAGCGG TGCCATGTCAGGGTTAGGGTTAGTTTTTTTTTAGTGTGCGCACTTGCACG TACGTGTCGGGGTTAGAGTTAGGGTCAGGCCAAGCGCTCCGTTGCTTTGA ACGGCAACGCATTACAGCAGTTATTGTTTGGTGTGTTGTTGTTGTGTGGC GTCGCAGCATGGTAAACAGAGTGCGCCACACGCTGCTGGCACCTTGCGCT GTTGCTGATTTGCATGCTCACCTGCACTCCGAGCGATGCCACAAGGTGAG CTTGACATGCTCGTAGCATCAATTTGATGTTGACCACTGTAGCCATGCAT TTCTTTCTTCATCAATCCTGTTTCTTGGTTTTTGTTGCCTCTGCATCAGG TGCTACCGGACCCCTTCGACGATGACTACGATGACCCCCTCGGGGGGTCA CGACTTCAAGTTGAAGTTTCCCTTCAACCGGGCGACCGGCTGGGACTGCG CCTGCGGGATATACAGGGGGGCGGCGTGCTGGTGATGGGGTTCATCACCA GGGGGTGCGCTGCGCAGGTGCGTCAGCTTTGAAGTTTACAGTTGCTTATG TACTCGAAGTACATGCTTTGAGGCTATACGGATGCAAATGCGATGTGATG TACTTACTCATGGTGCGCATACAGTATGTGAGGCATGTGCCATCATTGTA TGTTGTACACGTTTTTACATCCCTCGGCAGTAGCCCATTATCAATGTGGG GTGTGCTGCGCAGGTGCATACTCGTCAGCGGTCTGTCAGCGGCACAAGTC TTCTTCAGCACGGTTTAGTTTTGACACTTTTCTGATCTTGCTGCATCCGT TCAACTGGTTCCAGCCCATGGAGCCACTGCATTTTTGAAAGGGATCTGCC TATTGAGACGAGTACCTCACACCAGGGCAGTTTGACGCGACGCTTGCAAC AGCCCTTTGAGTCGACTAAAGGCTGTTCTCAGCTCAGTGCTGTAGCAATA ACAATTTTTCATTGTTGTATAAAAATGCCCGTACCAGTCACTTCATGCCC GAATGTGTGCGCTCAGCACAGCAACACATCACTCCTGCTCATTGCAACAT CAACTTCAACTTATTTTTGCACATCGCTGGCTGTTGCCACAGCGACCTGG TCACTTCTGCCTCATGCGCATACATACGGCTGGACCAAGCACAGCACACG CTCACTTCACCAGTGACTTCAACTCCTTCGTGCGCAGGTTGCTGGCGTCT GCCAAGGCGACCGAGTCACCCATATCGACGGCACTGGACCGCTATTATCA GCAGCAGAGGCGGCGGCCGCAGCAGCAGCAGCGCTCGACCGCTGCGGCGG TGGGGGAGTATTGAAGTTGACGCTCCGCAGGGGGTTC
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Coding sequence (CDS) from alignment at Contig_19:96491..101227+

>mRNA_5460 ID=mRNA_5460|Name=jgi.p|Trimin1|147988|organism=Tribonema minus UTEX_B_3156 |type=CDS|length=1059bp|location=Sequence derived from alignment at Contig_19:96491..101227+ (Tribonema minus UTEX_B_3156 )
GAGTTTGGTGCGCAAGACTTCCTGGAGCCCGGCCGGCAGTTTGTGCTGGA
GGGCGACCTTATTAAGATCACACGCGGCGGCCGCAAAGAGCACAAGTTCG
TGCTCTTCAATGACAAGCTCGTCTATGGCGAAGAGAAAGGCCTACTCATG
CGCCGCACGGGCATGTTTGCCAACGTCGTCAGCGGCAACACCAAGCGCCA
GTACCGCAAGGCCTACGACTTCCCCCTGAGCAGGGGGCTGTACGTGGTCA
ACGACGTGGGGGGTGCGTATGCCAGCGCGCGTAGCGGGGGAGGGGGCGGG
GGGGCAGCGGAGGGGGTTTTGGGGGACACGGGCTTCATGTTCATCACGCC
GGATAAGAGTTTTGTCGTGCACGCTGCGTCGCCTGCGGAGAAGGACGCGT
GGGTGCAGGCATTACGACGCGTGATGGCTGACCTGACGGCCAAGGGTGAC
TCGCGCGCGCTCAAGGTCGACATGGATCTGTCAGACAAGGTCAAGCCCGC
CAAAAGCGGCAAATCCATAAGCTGCGACATCTTCGTCTGGGTGATTCGCG
ACGGGAAGACCATGCGCCAGGGGTTCTCCCTCCCCCCGTCCGCCCTGTCC
GCCCCCTTTGGCGTCCTCTCGTACAGCGAGCTGGTCCAGAGGGGGGCGCA
AGTGGACCCCTCCCCCACCCGCGCTGGCCTTCCTGATGACGATCTAGGGT
TCGGTCTGGAGTGGGATGCGCCCCCCGCACTTCAACTTGAGGTGCTACCG
GACCCCTTCGACGATGACTACGATGACCCCCTCGGGGGGTCACGACTTCA
AGTTGAAGTTTCCCTTCAACCGGGCGACCGGCTGGGACTGCGCCTGCGGG
ATATACAGGGGGGCGGCGTGCTGGTGATGGGGTTCATCACCAGGGGGTGC
GCTGCGCAGGTTGCTGGCGTCTGCCAAGGCGACCGAGTCACCCATATCGA
CGGCACTGGACCGCTATTATCAGCAGCAGAGGCGGCGGCCGCAGCAGCAG
CAGCGCTCGACCGCTGCGGCGGTGGGGGAGTATTGAAGTTGACGCTCCGC
AGGGGGTTC
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