mRNA_3179 (mRNA) Tribonema minus UTEX_B_3156

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
Namejgi.p|Trimin1|160751
Unique NamemRNA_3179
TypemRNA
OrganismTribonema minus UTEX_B_3156 (Tribonema minus UTEX_B_3156 )
Homology
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BLAST of jgi.p|Trimin1|160751 vs. uniprot
Match: A0A835ZFQ8_9STRA (U-box domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZFQ8_9STRA)

HSP 1 Score: 560 bits (1444), Expect = 3.200e-201
Identity = 298/298 (100.00%), Postives = 298/298 (100.00%), Query Frame = 1 
Query:    1 MGRPASGLQRSQASNNIRLAARCLREKFGESTRSFVAQGIFIITRLRNSRPDALELAQGKKLVAYVNEGQLALACLPLFETGRIEERFSLSTLEGTLRKISEARMALLHATKKRSRCNATSTSAASRARASYPPATGRSVSDGEAELEDAHEPITKRRRNHTYGGGEGARLAKMAAEADASCKHANAERPADQRAEDDVKQGPVVTKLRAAGVAVPSEFVCPLTGDVMREPVLLTGDQRHYDSKACAKWLISNSVSPSTGEPLGACSFMVPAHALRQRIAAFVEEHASVLSVTVTAQG 894
            MGRPASGLQRSQASNNIRLAARCLREKFGESTRSFVAQGIFIITRLRNSRPDALELAQGKKLVAYVNEGQLALACLPLFETGRIEERFSLSTLEGTLRKISEARMALLHATKKRSRCNATSTSAASRARASYPPATGRSVSDGEAELEDAHEPITKRRRNHTYGGGEGARLAKMAAEADASCKHANAERPADQRAEDDVKQGPVVTKLRAAGVAVPSEFVCPLTGDVMREPVLLTGDQRHYDSKACAKWLISNSVSPSTGEPLGACSFMVPAHALRQRIAAFVEEHASVLSVTVTAQG
Sbjct:    1 MGRPASGLQRSQASNNIRLAARCLREKFGESTRSFVAQGIFIITRLRNSRPDALELAQGKKLVAYVNEGQLALACLPLFETGRIEERFSLSTLEGTLRKISEARMALLHATKKRSRCNATSTSAASRARASYPPATGRSVSDGEAELEDAHEPITKRRRNHTYGGGEGARLAKMAAEADASCKHANAERPADQRAEDDVKQGPVVTKLRAAGVAVPSEFVCPLTGDVMREPVLLTGDQRHYDSKACAKWLISNSVSPSTGEPLGACSFMVPAHALRQRIAAFVEEHASVLSVTVTAQG 298
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BLAST of jgi.p|Trimin1|160751 vs. uniprot
Match: A0A835Z8R9_9STRA (U-box domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z8R9_9STRA)

HSP 1 Score: 245 bits (626), Expect = 9.490e-77
Identity = 154/293 (52.56%), Postives = 197/293 (67.24%), Query Frame = 1 
Query:    1 MGRPASGLQRSQASNNIRLAARCLREKFGESTRSFVAQGIFIITRLRNSRPDALELAQGKKLVAYVNEGQLALACLPLFETGRIE-ERFSLSTLEGTLRKISEARMALLHATKKRSRCNATSTSAASRARASYPPATGRSVSDGEAELEDAHEPITKRRRNHTYGGGEGARLAKMAAEADASCKHANAERPADQRAEDDVKQG--PVVTKLRAAGVAVPSEFVCPLTGDVMREPVLLTGDQRHYDSKACAKWLISNSVSPSTGEPLGACSFMVPAHALRQRIAAFVEEHASVL 870
            M  P SG   SQ S  +  AAR LREKFG+ST+SFVAQG  II+RLR SRPDAL   QGKKL+ YVNEGQ AL CLPL E G ++ E  +L++LE T++KISEAR+ALLHAT+KR+R  A  + +A+    ++        SDGE+    A     +RRR H+Y G +G+ LA  AA  D        E P  Q  +  ++ G  P+V +LRAAGV VP EFVC LT DVMR+PVLLTGD RHY+S ACA+WL+S++ SP+T   L AC+FM  AHAL+QRI AF++EH+ +L
Sbjct:    9 MRAPHSGFSGSQLS--VTQAARGLREKFGDSTKSFVAQGNLIISRLRASRPDALSAPQGKKLLTYVNEGQKALDCLPLLEAGEMQQEGNALASLEATMQKISEARVALLHATRKRTRAAAIRSHSATSDTGAF--------SDGESR--SAAPAPKRRRRPHSYAGHDGSWLAAAAANGD--------ETPLTQGLDLSLRAGSIPLVARLRAAGVKVPPEFVCALTNDVMRDPVLLTGDGRHYESSACARWLVSHNTSPTTHAQLPACNFMARAHALKQRIWAFLDEHSDIL 281
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BLAST of jgi.p|Trimin1|160751 vs. uniprot
Match: A0A7S0E165_9CRYP (Hypothetical protein n=1 Tax=Hanusia phi TaxID=3032 RepID=A0A7S0E165_9CRYP)

HSP 1 Score: 66.2 bits (160), Expect = 1.250e-9
Identity = 36/82 (43.90%), Postives = 53/82 (64.63%), Query Frame = 1 
Query:  610 VVTKLRAAGVAVPSEFVCPLTGDVMREPVLLTGDQRHYDSKACAKWLISNSVSPSTGEPLGACSFMVPAHALRQRIAAFVEE 855
            + T L   GV+VPSE++CP+T ++MR+PV+LT D   Y+  A  +WL  +  SP T + +   S ++P HALR  IA FVE+
Sbjct:   54 IKTLLEERGVSVPSEYLCPITMELMRDPVILT-DGHVYERAAIDRWLRHHCTSPKTNQIVNR-SQIIPCHALRVIIAEFVEQ 133
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BLAST of jgi.p|Trimin1|160751 vs. uniprot
Match: A0A7S0YUT2_9CRYP (Hypothetical protein n=1 Tax=Hemiselmis tepida TaxID=464990 RepID=A0A7S0YUT2_9CRYP)

HSP 1 Score: 63.2 bits (152), Expect = 7.010e-9
Identity = 32/75 (42.67%), Postives = 47/75 (62.67%), Query Frame = 1 
Query:  634 GVAVPSEFVCPLTGDVMREPVLLTGDQRHYDSKACAKWLISNSVSPSTGEPLGACSFMVPAHALRQRIAAFVEEH 858
            G+ VP  F+CP++ +VM +PV+LT D   Y+ K   +WL+S+  SP T + + A   ++P HALR  I  FVE H
Sbjct:   34 GIPVPDAFLCPISMEVMSDPVILT-DGHVYERKTIERWLLSHDTSPKTNQVV-ATDQLIPCHALRTVIQDFVETH 106
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BLAST of jgi.p|Trimin1|160751 vs. uniprot
Match: A0A7S4J9M5_GUITH (Hypothetical protein n=1 Tax=Guillardia theta TaxID=55529 RepID=A0A7S4J9M5_GUITH)

HSP 1 Score: 66.6 bits (161), Expect = 1.290e-8
Identity = 35/82 (42.68%), Postives = 54/82 (65.85%), Query Frame = 1 
Query:  610 VVTKLRAAGVAVPSEFVCPLTGDVMREPVLLTGDQRHYDSKACAKWLISNSVSPSTGEPLGACSFMVPAHALRQRIAAFVEE 855
            + T L   G++VP+E++CP+T ++MR+PV+LT D   Y+  A  +WL  +S SP T + +   S ++P HALR  IA FVE+
Sbjct:  507 IKTLLEERGLSVPTEYLCPITMEIMRDPVILT-DGHVYERAAIDRWLRHHSTSPKTNQIVNR-SQIIPCHALRVIIAEFVEQ 586
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BLAST of jgi.p|Trimin1|160751 vs. uniprot
Match: R1D2B9_EMIHU (U-box domain-containing protein (Fragment) n=2 Tax=Emiliania huxleyi TaxID=2903 RepID=R1D2B9_EMIHU)

HSP 1 Score: 56.2 bits (134), Expect = 6.520e-7
Identity = 30/72 (41.67%), Postives = 42/72 (58.33%), Query Frame = 1 
Query:  646 PSEFVCPLTGDVMREPVLLTGDQRHYDSKACAKWLISNSVSPSTGEPLGACSFMVPAHALRQRIAAFVEEHA 861
            P +FVCP+T +VM +PV+   D   Y+  A  +WL S S SP TG  L     ++P H LR++I  + E HA
Sbjct:    3 PHDFVCPITTEVMSDPVM-AADGHSYERSAIERWLASKSTSPLTGGELEH-RILIPNHTLRRQIGEWHEAHA 72
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BLAST of jgi.p|Trimin1|160751 vs. uniprot
Match: A0A7S2BC43_9EUKA (Hypothetical protein n=1 Tax=Haptolina brevifila TaxID=156173 RepID=A0A7S2BC43_9EUKA)

HSP 1 Score: 60.1 bits (144), Expect = 9.950e-7
Identity = 31/65 (47.69%), Postives = 44/65 (67.69%), Query Frame = 1 
Query:  643 VPSEFVCPLTGDVMREPVLLTGDQRHYDSKACAKWLISNSVSPSTGEPLGACSFMVPAHALRQRI 837
            +P EF+CP+TG+VMR+PV  T D   ++ +A  +WL+++S SP TG PL     + PA ALRQ I
Sbjct:  227 IPPEFLCPITGEVMRDPVT-TADGHAFERRAIERWLLTHSSSPMTGAPLPHKQ-LAPAIALRQLI 289
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BLAST of jgi.p|Trimin1|160751 vs. uniprot
Match: A0A7S3L6F9_9STRA (Hypothetical protein (Fragment) n=1 Tax=Amphora coffeiformis TaxID=265554 RepID=A0A7S3L6F9_9STRA)

HSP 1 Score: 57.8 bits (138), Expect = 1.720e-6
Identity = 34/82 (41.46%), Postives = 47/82 (57.32%), Query Frame = 1 
Query:  613 VTKLRAAGVAVPSEFVCPLTGDVMREPVLLTGDQRHYDSKACAKWLISNSVSPSTGEPLGACSFMVPAHALRQRIAAFVEEH 858
            +T      V VP E++CPLT DVM EP LL+ +  +Y+ +A   W+  N  SP T EPL   S +V   AL+ RI  F+ +H
Sbjct:   17 ITSADGTLVPVPEEYICPLTLDVMAEP-LLSREGHNYEREAILNWVSENGTSPLTREPLRP-SQLVLNRALKFRIQFFLRQH 96
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BLAST of jgi.p|Trimin1|160751 vs. uniprot
Match: A0A7S3T7N2_EMIHU (Hypothetical protein n=1 Tax=Emiliania huxleyi TaxID=2903 RepID=A0A7S3T7N2_EMIHU)

HSP 1 Score: 55.8 bits (133), Expect = 2.980e-6
Identity = 28/72 (38.89%), Postives = 43/72 (59.72%), Query Frame = 1 
Query:  646 PSEFVCPLTGDVMREPVLLTGDQRHYDSKACAKWLISNSVSPSTGEPLGACSFMVPAHALRQRIAAFVEEHA 861
            P +F+CP+T ++M +PV+   D + Y+  A  +WL S S SP TG  L     ++P H LR++I  + E HA
Sbjct:   54 PDDFICPITTEIMSDPVV-AADGQSYERSAIERWLASKSTSPLTGGELEH-RILIPNHTLRRQIGEWHEAHA 123
[Close]
BLAST of jgi.p|Trimin1|160751 vs. uniprot
Match: R1B7B1_EMIHU (U-box domain-containing protein n=1 Tax=Emiliania huxleyi TaxID=2903 RepID=R1B7B1_EMIHU)

HSP 1 Score: 54.7 bits (130), Expect = 4.280e-6
Identity = 29/71 (40.85%), Postives = 42/71 (59.15%), Query Frame = 1 
Query:  646 PSEFVCPLTGDVMREPVLLTGDQRHYDSKACAKWLISNSVSPSTGEPLGACSFMVPAHALRQRIAAFVEEH 858
            P EF CP+T ++M +PV+   D + Y+  A  +WL + S SP TGE L   +   P H LR++I A+ E H
Sbjct:   28 PDEFGCPITTELMSDPVM-AADGQSYERSAIERWLATKSTSPMTGEALE-YTHTFPNHLLRRQIRAWQEAH 96
The following BLAST results are available for this feature:
BLAST of jgi.p|Trimin1|160751 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A835ZFQ8_9STRA3.200e-201100.00U-box domain-containing protein n=1 Tax=Tribonema ... [more]
A0A835Z8R9_9STRA9.490e-7752.56U-box domain-containing protein n=1 Tax=Tribonema ... [more]
A0A7S0E165_9CRYP1.250e-943.90Hypothetical protein n=1 Tax=Hanusia phi TaxID=303... [more]
A0A7S0YUT2_9CRYP7.010e-942.67Hypothetical protein n=1 Tax=Hemiselmis tepida Tax... [more]
A0A7S4J9M5_GUITH1.290e-842.68Hypothetical protein n=1 Tax=Guillardia theta TaxI... [more]
R1D2B9_EMIHU6.520e-741.67U-box domain-containing protein (Fragment) n=2 Tax... [more]
A0A7S2BC43_9EUKA9.950e-747.69Hypothetical protein n=1 Tax=Haptolina brevifila T... [more]
A0A7S3L6F9_9STRA1.720e-641.46Hypothetical protein (Fragment) n=1 Tax=Amphora co... [more]
A0A7S3T7N2_EMIHU2.980e-638.89Hypothetical protein n=1 Tax=Emiliania huxleyi Tax... [more]
R1B7B1_EMIHU4.280e-640.85U-box domain-containing protein n=1 Tax=Emiliania ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Contig_14contigContig_14:594344..595701 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Tribonema minus UTEX_B_31562021-06-17
Properties
Property NameValue
ProteomeIdTrimin1|160751|e_gw1.14.85.1
Descriptionubiquitin-protein transferase activity
COG categoryS
Max annot lvl2759|Eukaryota
EggNOG OGs2E1VG@1|root,2S95A@2759|Eukaryota
Evalue1.07e-09
Seed ortholog2903.EOD06267
Hectar predicted targeting categoryother localisation
TranscriptId161073
TrackFilteredModels1
ProteinId160751
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameSpeciesTypePosition
jgi.p|Trimin1|160751gene_3179Tribonema minus UTEX_B_3156 geneContig_14 594344..595701 +


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
exon_3179_1exon_3179_1Tribonema minus UTEX_B_3156 exonContig_14 594344..594649 +
exon_3179_2exon_3179_2Tribonema minus UTEX_B_3156 exonContig_14 594859..595268 +
exon_3179_3exon_3179_3Tribonema minus UTEX_B_3156 exonContig_14 595521..595701 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
CDS_3179CDS_3179Tribonema minus UTEX_B_3156 CDSContig_14 594344..594649 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
jgi.p|Trimin1|160751Trimin1|160751|e_gw1.14.85.1Tribonema minus UTEX_B_3156 polypeptideContig_14 594344..595701 +


Sequences
The following sequences are available for this feature:

spliced messenger RNA

>mRNA_3179 ID=mRNA_3179|Name=jgi.p|Trimin1|160751|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=897bp|location=Sequence derived from alignment at Contig_14:594344..595701+ (Tribonema minus UTEX_B_3156 )|Notes=Excludes all bases but those of type(s): exon.  
ATGGGCCGTCCAGCATCAGGACTGCAGAGGTCACAAGCCTCGAACAACAT
CCGGCTTGCAGCACGCTGTCTCAGGGAAAAGTTTGGGGAGAGCACTCGGA
GCTTTGTGGCCCAAGGCATCTTCATCATCACACGTTTGCGCAACAGCAGA
CCGGATGCATTGGAGCTTGCACAAGGGAAAAAGCTGGTAGCATACGTCAA
TGAAGGACAATTGGCACTGGCATGCCTGCCGCTCTTTGAGACAGGACGCA
TCGAGGAGCGCTTCTCCTTGTCCACACTGGAGGGCACTCTGCGAAAAATC
AGCGAGGCCCGTATGGCACTGCTGCATGCCACCAAGAAGCGCTCGCGCTG
CAACGCCACCAGCACCAGCGCCGCCAGCCGCGCGCGCGCCTCGTACCCAC
CCGCCACAGGCCGCTCAGTGTCAGACGGCGAGGCGGAGCTTGAGGATGCC
CACGAGCCAATCACCAAGCGGCGCCGCAACCACACGTACGGTGGCGGCGA
GGGCGCCCGACTCGCCAAGATGGCTGCTGAAGCTGACGCCTCATGCAAGC
ACGCAAATGCCGAGAGACCCGCAGATCAGCGGGCCGAAGATGACGTCAAG
CAGGGGCCGGTGGTCACAAAGCTGCGCGCGGCAGGCGTCGCCGTGCCATC
CGAGTTTGTGTGCCCGCTCACCGGGGATGTGATGCGTGAGCCGGTTCTGC
TTACGGGAGACCAAAGGCACTACGACAGCAAAGCGTGCGCGAAGTGGCTC
ATCAGCAACTCTGTCAGCCCGTCAACAGGCGAGCCGCTGGGTGCGTGCAG
CTTCATGGTCCCGGCGCACGCGTTGCGCCAACGGATCGCCGCATTTGTCG
AGGAACACGCTTCTGTACTCAGCGTCACCGTCACGGCACAGGGATGA
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protein sequence of jgi.p|Trimin1|160751

>Trimin1|160751|e_gw1.14.85.1 ID=Trimin1|160751|e_gw1.14.85.1|Name=jgi.p|Trimin1|160751|organism=Tribonema minus UTEX_B_3156 |type=polypeptide|length=299bp
MGRPASGLQRSQASNNIRLAARCLREKFGESTRSFVAQGIFIITRLRNSR
PDALELAQGKKLVAYVNEGQLALACLPLFETGRIEERFSLSTLEGTLRKI
SEARMALLHATKKRSRCNATSTSAASRARASYPPATGRSVSDGEAELEDA
HEPITKRRRNHTYGGGEGARLAKMAAEADASCKHANAERPADQRAEDDVK
QGPVVTKLRAAGVAVPSEFVCPLTGDVMREPVLLTGDQRHYDSKACAKWL
ISNSVSPSTGEPLGACSFMVPAHALRQRIAAFVEEHASVLSVTVTAQG*
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mRNA from alignment at Contig_14:594344..595701+

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_3179 ID=mRNA_3179|Name=jgi.p|Trimin1|160751|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=1358bp|location=Sequence derived from alignment at Contig_14:594344..595701+ (Tribonema minus UTEX_B_3156 )
ATGGGCCGTCCAGCATCAGGACTGCAGAGGTCACAAGCCTCGAACAACAT
CCGGCTTGCAGCACGCTGTCTCAGGGAAAAGTTTGGGGAGAGCACTCGGA
GCTTTGTGGCCCAAGGCATCTTCATCATCACACGTTTGCGCAACAGCAGA
CCGGATGCATTGGAGCTTGCACAAGGGAAAAAGCTGGTAGCATACGTCAA
TGAAGGACAATTGGCACTGGCATGCCTGCCGCTCTTTGAGACAGGACGCA
TCGAGGAGCGCTTCTCCTTGTCCACACTGGAGGGCACTCTGCGAAAAATC
AGCGAGGTGAGTTGCAGGTGTCGCGCAGCGCTGAGTCGTCTCAGAATAAC
GCATGGAGCTCGCTCTACCTTTGTTGGCAAAATGCGACAGACGTGGTGCT
GTAGAATGGTTGCTGTGGGCACACATCACTCTCCAGCACGCTGCTGCGCT
GCTGCGATCAAGCTTCCGCACTGCTAACAAGCTGCTGCATCGCTGCTGCT
GCCGTTTCGACGCAGGCCCGTATGGCACTGCTGCATGCCACCAAGAAGCG
CTCGCGCTGCAACGCCACCAGCACCAGCGCCGCCAGCCGCGCGCGCGCCT
CGTACCCACCCGCCACAGGCCGCTCAGTGTCAGACGGCGAGGCGGAGCTT
GAGGATGCCCACGAGCCAATCACCAAGCGGCGCCGCAACCACACGTACGG
TGGCGGCGAGGGCGCCCGACTCGCCAAGATGGCTGCTGAAGCTGACGCCT
CATGCAAGCACGCAAATGCCGAGAGACCCGCAGATCAGCGGGCCGAAGAT
GACGTCAAGCAGGGGCCGGTGGTCACAAAGCTGCGCGCGGCAGGCGTCGC
CGTGCCATCCGAGTTTGTGTGCCCGCTCACCGGGGATGTGATGCGTGAGC
CGGTTCTGCTTACGGGAGACCAAAGGTGGGTCACCTATGTACTGTGCATG
ATGTTCATGCAGTGTTGTGGTTGTGCGTCGACGTTTGTGTACAACAGCGA
TGCTGCTGCTATGTACAGCATTCAGTCATGTGCACGAGACATGTCACATG
TGTCATCATGGAGGCCTGCCTGTACCTTAGTTTTCACCTGCTCGACACAT
ACTGCGCATCGCTCACTGGCTTGCACGAGCTCATCAATCGGCATCAGCTC
ATCACTCTCTGTGCTTGCCCTCTGCAGGCACTACGACAGCAAAGCGTGCG
CGAAGTGGCTCATCAGCAACTCTGTCAGCCCGTCAACAGGCGAGCCGCTG
GGTGCGTGCAGCTTCATGGTCCCGGCGCACGCGTTGCGCCAACGGATCGC
CGCATTTGTCGAGGAACACGCTTCTGTACTCAGCGTCACCGTCACGGCAC
AGGGATGA
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Coding sequence (CDS) from alignment at Contig_14:594344..595701+

>mRNA_3179 ID=mRNA_3179|Name=jgi.p|Trimin1|160751|organism=Tribonema minus UTEX_B_3156 |type=CDS|length=897bp|location=Sequence derived from alignment at Contig_14:594344..595701+ (Tribonema minus UTEX_B_3156 )
ATGGGCCGTCCAGCATCAGGACTGCAGAGGTCACAAGCCTCGAACAACAT
CCGGCTTGCAGCACGCTGTCTCAGGGAAAAGTTTGGGGAGAGCACTCGGA
GCTTTGTGGCCCAAGGCATCTTCATCATCACACGTTTGCGCAACAGCAGA
CCGGATGCATTGGAGCTTGCACAAGGGAAAAAGCTGGTAGCATACGTCAA
TGAAGGACAATTGGCACTGGCATGCCTGCCGCTCTTTGAGACAGGACGCA
TCGAGGAGCGCTTCTCCTTGTCCACACTGGAGGGCACTCTGCGAAAAATC
AGCGAGGCCCGTATGGCACTGCTGCATGCCACCAAGAAGCGCTCGCGCTG
CAACGCCACCAGCACCAGCGCCGCCAGCCGCGCGCGCGCCTCGTACCCAC
CCGCCACAGGCCGCTCAGTGTCAGACGGCGAGGCGGAGCTTGAGGATGCC
CACGAGCCAATCACCAAGCGGCGCCGCAACCACACGTACGGTGGCGGCGA
GGGCGCCCGACTCGCCAAGATGGCTGCTGAAGCTGACGCCTCATGCAAGC
ACGCAAATGCCGAGAGACCCGCAGATCAGCGGGCCGAAGATGACGTCAAG
CAGGGGCCGGTGGTCACAAAGCTGCGCGCGGCAGGCGTCGCCGTGCCATC
CGAGTTTGTGTGCCCGCTCACCGGGGATGTGATGCGTGAGCCGGTTCTGC
TTACGGGAGACCAAAGGCACTACGACAGCAAAGCGTGCGCGAAGTGGCTC
ATCAGCAACTCTGTCAGCCCGTCAACAGGCGAGCCGCTGGGTGCGTGCAG
CTTCATGGTCCCGGCGCACGCGTTGCGCCAACGGATCGCCGCATTTGTCG
AGGAACACGCTTCTGTACTCAGCGTCACCGTCACGGCACAGGGATGA
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