mRNA_13816 (mRNA) Tribonema minus UTEX_B_3156

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview


Name	jgi.p\|Trimin1\|176367
Unique Name	mRNA_13816
Type	mRNA
Organism	Tribonema minus UTEX_B_3156 (Tribonema minus UTEX_B_3156 )

Homology

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BLAST of jgi.p|Trimin1|176367 vs. uniprot
Match: A0A835ZEK7_9STRA (Sodium/hydrogen exchanger family-domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZEK7_9STRA)

HSP 1 Score: 1155 bits (2987), Expect = 0.000e+0
Identity = 621/621 (100.00%), Postives = 621/621 (100.00%), Query Frame = 1

Query:    1 MTGLEGEVTSLAALGADSLIFLAATVFLVPLSKKLNVSPVLGFLGAGLALGPHGFKLIRELRDLNALGELGITFLLFEQGLELSLDRLKALGKYAFGLGTLQVLLCTLAFGAFPFGEQSSVQLMESIFKSPDSLVNISRLDESVVIGAALSLSSSAFVLKILQEKGLLSGRAGQATLGVLLLQDIAVVPLLVLLPIIENQQAGAGDVPLMAQAAVLGGIALKAVGGLGAILVFGRFLLRRLFDGVAATRSTEAFVALCLLVAIGIGEVTDAMGLSASLGAFVAGTLLAETNYRMQVEADIAPFRGLLLALFFMTTGASVDPHMLWEQWPTVLGLLTGLLLVKGIITSTLGPLFGLTKGESVRVGSILSGGGEFAFVVLTLAQKLNVLPLALDKLLVGTVVLSMALTPVLAAAGDKLGDYFDTQAGHSAPGAGFDDETLASPIGPFEGQDLVVICGFGPVGQIVASLISAPAVSGALCGARFVAFDLDPRRVSAARARGYPVFYGDGSQPKVIETAGIADPRALVVTYSDHALALSAVERLRGAYPRAKVFARATDYGQYWRLRREGATAVVSDLSEISIRLGSELLESFGVARTYEIQSAKAELRETLACLSQVRQKKLQS 1863
            MTGLEGEVTSLAALGADSLIFLAATVFLVPLSKKLNVSPVLGFLGAGLALGPHGFKLIRELRDLNALGELGITFLLFEQGLELSLDRLKALGKYAFGLGTLQVLLCTLAFGAFPFGEQSSVQLMESIFKSPDSLVNISRLDESVVIGAALSLSSSAFVLKILQEKGLLSGRAGQATLGVLLLQDIAVVPLLVLLPIIENQQAGAGDVPLMAQAAVLGGIALKAVGGLGAILVFGRFLLRRLFDGVAATRSTEAFVALCLLVAIGIGEVTDAMGLSASLGAFVAGTLLAETNYRMQVEADIAPFRGLLLALFFMTTGASVDPHMLWEQWPTVLGLLTGLLLVKGIITSTLGPLFGLTKGESVRVGSILSGGGEFAFVVLTLAQKLNVLPLALDKLLVGTVVLSMALTPVLAAAGDKLGDYFDTQAGHSAPGAGFDDETLASPIGPFEGQDLVVICGFGPVGQIVASLISAPAVSGALCGARFVAFDLDPRRVSAARARGYPVFYGDGSQPKVIETAGIADPRALVVTYSDHALALSAVERLRGAYPRAKVFARATDYGQYWRLRREGATAVVSDLSEISIRLGSELLESFGVARTYEIQSAKAELRETLACLSQVRQKKLQS
Sbjct:    1 MTGLEGEVTSLAALGADSLIFLAATVFLVPLSKKLNVSPVLGFLGAGLALGPHGFKLIRELRDLNALGELGITFLLFEQGLELSLDRLKALGKYAFGLGTLQVLLCTLAFGAFPFGEQSSVQLMESIFKSPDSLVNISRLDESVVIGAALSLSSSAFVLKILQEKGLLSGRAGQATLGVLLLQDIAVVPLLVLLPIIENQQAGAGDVPLMAQAAVLGGIALKAVGGLGAILVFGRFLLRRLFDGVAATRSTEAFVALCLLVAIGIGEVTDAMGLSASLGAFVAGTLLAETNYRMQVEADIAPFRGLLLALFFMTTGASVDPHMLWEQWPTVLGLLTGLLLVKGIITSTLGPLFGLTKGESVRVGSILSGGGEFAFVVLTLAQKLNVLPLALDKLLVGTVVLSMALTPVLAAAGDKLGDYFDTQAGHSAPGAGFDDETLASPIGPFEGQDLVVICGFGPVGQIVASLISAPAVSGALCGARFVAFDLDPRRVSAARARGYPVFYGDGSQPKVIETAGIADPRALVVTYSDHALALSAVERLRGAYPRAKVFARATDYGQYWRLRREGATAVVSDLSEISIRLGSELLESFGVARTYEIQSAKAELRETLACLSQVRQKKLQS 621

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BLAST of jgi.p|Trimin1|176367 vs. uniprot
Match: A0A835ZF36_9STRA (K(+) efflux antiporter 3 n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZF36_9STRA)

HSP 1 Score: 868 bits (2242), Expect = 6.860e-309
Identity = 475/613 (77.49%), Postives = 532/613 (86.79%), Query Frame = 1

Query:    1 MTGLEGEVTSLAALGADSLIFLAATVFLVPLSKKLNVSPVLGFLGAGLALGPHGFKLIRELRDLNALGELGITFLLFEQGLELSLDRLKALGKYAFGLGTLQVLLCTLAFGAFPFGEQSSVQLMESIFKSPDSLVNISRLDESVVIGAALSLSSSAFVLKILQEKGLLSGRAGQATLGVLLLQDIAVVPLLVLLPIIENQQAGAGDVPLMAQAAVLGGIALKAVGGLGAILVFGRFLLRRLFDGVAATRSTEAFVALCLLVAIGIGEVTDAMGLSASLGAFVAGTLLAETNYRMQVEADIAPFRGLLLALFFMTTGASVDPHMLWEQWPTVLGLLTGLLLVKGIITSTLGPLFGLTKGESVRVGSILSGGGEFAFVVLTLAQKLNVLPLALDKLLVGTVVLSMALTPVLAAAGDKLGDYFDTQAGHSAPGAGFDDETLASPIGPFEGQDLVVICGFGPVGQIVASLISAPAVSGALCGARFVAFDLDPRRVSAARARGYPVFYGDGSQPKVIETAGIADPRALVVTYSDHALALSAVERLRGAYPRAKVFARATDYGQYWRLRREGATAVVSDLSEISIRLGSELLESFGVARTYEIQSAKAELRETLACLSQ 1839
            M+GLEG+VT +AALG D+L FLAATV +VP++K+ N+SPVLGFL AGL LGP G   I++LRDLNALGELGITFLLFEQGLELSLDRLKALGKYAFGLGTLQVLLCT AFGAFPF     VQL+E +FKSPD+LVNISRLDE+VVIGAALSLSSSAFVLKILQEKGLL GRAGQATLGVLLLQDIAVVPLLVLLPIIENQQ    DVPL  QA++L   A+KA+GGL  ILV GR +LRRLF+ VAA RSTE FVALCLLVAIGIGE+TD++GLS++LGAF+AGTLLAETNYRMQVEADIAPFRGLLL LFFMTTGASVDP +L EQWPTV+GLL GLLL+KG+ITS + PLFGLTKGESVRVGSILSGGGEFAFVVLTLAQKLNVLP+ LDKLLVGTVVLSMALTPVLA AGD+LG Y D     +   +  +D    +P G  EG+D+VVICGFGPVGQIV SLIS+P VS AL  A+F+AFDL+P+RVS +R +GYPV+YGDGSQPKVIETAGI +P+ALVVTYS+H LA+SAVERLR AYP   +F RATDY QYWRL+ EGATAVVSDLSEISIRLGSELLESFGV RTY+IQ+AK ELR+TLA LSQ
Sbjct:  100 MSGLEGDVTGMAALGTDALKFLAATVAIVPIAKRFNLSPVLGFLAAGLILGPKGVGAIKDLRDLNALGELGITFLLFEQGLELSLDRLKALGKYAFGLGTLQVLLCTAAFGAFPF--VGGVQLLELVFKSPDALVNISRLDEAVVIGAALSLSSSAFVLKILQEKGLLRGRAGQATLGVLLLQDIAVVPLLVLLPIIENQQR---DVPLGLQASMLALTAVKAIGGLAGILVLGRVILRRLFEAVAAARSTETFVALCLLVAIGIGELTDSLGLSSTLGAFIAGTLLAETNYRMQVEADIAPFRGLLLGLFFMTTGASVDPGLLLEQWPTVIGLLGGLLLIKGVITSLVCPLFGLTKGESVRVGSILSGGGEFAFVVLTLAQKLNVLPMTLDKLLVGTVVLSMALTPVLAEAGDRLGAYLDELDEQNGVKSAEED----NPEG-VEGRDMVVICGFGPVGQIVGSLISSPTVSNALNNAKFIAFDLNPKRVSESRKKGYPVYYGDGSQPKVIETAGINNPKALVVTYSNHDLAVSAVERLRAAYPLTPIFTRATDYEQYWRLQEEGATAVVSDLSEISIRLGSELLESFGVPRTYDIQAAKVELRQTLARLSQ 702

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BLAST of jgi.p|Trimin1|176367 vs. uniprot
Match: A0A6H5JAW2_9PHAE (RCK N-terminal domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JAW2_9PHAE)

HSP 1 Score: 677 bits (1748), Expect = 4.910e-233
Identity = 389/618 (62.94%), Postives = 468/618 (75.73%), Query Frame = 1

Query:    7 GLEGEVTSLAALGADSLIFLAATVFLVPLSKKLNVSPVLGFLGAGLALGPHGFKLIRELRDLNALGELGITFLLFEQGLELSLDRLKALGKYAFGLGTLQVLLCTLAFGAFPFGEQSSVQLMESIFKSPDSLVNISRLDESVVIGAALSLSSSAFVLKILQEKGLLSGRAGQATLGVLLLQDIAVVPLLVLLPIIENQQAGAGDVPLMAQAAVLGGIALKAVGGLGAILVFGRFLLRRLFDGVAATRSTEAFVALCLLVAIGIGEVTDAMGLSASLGAFVAGTLLAETNYRMQVEADIAPFRGLLLALFFMTTGASVDPHMLWEQWPTVLGLLTGLLLVKGIITSTLGPLFGLTKGESVRVGSILSGGGEFAFVVLTLAQKLNVLPLALDKLLVGTVVLSMALTPVLAAAGDKLGDYF---DTQAGHSAPGAGFDDETLASPIGPFEG-----QDLVVICGFGPVGQIVASLISAPAVSGALCGARFVAFDLDPRRVSAARARGYPVFYGDGSQPKVIETAGIADPRALVVTYSDHALALSAVERLRGAYPRAKVFARATDYGQYWRLRREGATAVVSDLSEISIRLGSELLESFGVARTYEIQSAKAELRETLACLS 1836
            G+  +V  L  LG D+L+FLAATV +VP  KKLN+SPVLGFL  G  LGP G  +I+ L +L+ LGELGI+FLLFEQGLELSLDRLKAL KYAFGLGT QVLLCTLAF AFPF     V ++E IF SP  LV+I R+DE+VVIGAALSLSSSAFVLKILQEKG  + R G+A LG+LLLQDIAVVPLLVLLPIIE Q  G  D  L  Q  ++G    K VGGLG ILV GRFLLR LFD VA  RS+E FVALCLLVA+G+G++T+A+GLS +LGAF AGTLLAETNYR QVEA+IAPFRGLLL LFF TTGA+VDP +L EQWPTV GLL GL+  K II S+    FGL+  E+ + G ILSGGGEFAFVVL+L+Q+L VLP  L++LL+G VVLSMALTP L+  GD   +      T+ G ++  AG +   L  P G  EG     Q+ VVICGFGPVGQIV SL+S P +   L GA +VAFDL+P+RV  +  +G+PVFYGDG++P+V++TAG+ +P+A+VVTYS+  ++  AVERL  A+P + +F RATDY QY  LR  GATAVVSDLSEISIRLGSELLESFGVAR+ ++QSAK +LR+TL  L+
Sbjct:  164 GMAADV--LTTLGRDALMFLAATVAVVPACKKLNISPVLGFLATGAILGPSGISVIKNLNELDTLGELGISFLLFEQGLELSLDRLKALSKYAFGLGTAQVLLCTLAFAAFPF--LGGVDVLEVIFHSPAELVSIRRVDEAVVIGAALSLSSSAFVLKILQEKGQKAARFGRAVLGILLLQDIAVVPLLVLLPIIETQ--GGTDTALSEQLLLVGLTVAKGVGGLGGILVAGRFLLRPLFDVVAGARSSETFVALCLLVALGMGQLTEAIGLSTTLGAFAAGTLLAETNYRTQVEANIAPFRGLLLGLFFTTTGATVDPQLLVEQWPTVTGLLLGLVTFKSIIISSASLGFGLSAPEAAKTGLILSGGGEFAFVVLSLSQRLGVLPDELNRLLIGVVVLSMALTPALSELGDLASEKLIEVATKRGMASAVAGLE---LKGPAGEGEGKGLGGQEKVVICGFGPVGQIVGSLLSTPVIKDKL-GADYVAFDLNPKRVRQSMEQGFPVFYGDGTEPEVLKTAGMGNPKAIVVTYSNKEVSNQAVERLHQAFPGSPIFVRATDYEQYLSLRDFGATAVVSDLSEISIRLGSELLESFGVARSDDVQSAKTDLRKTLVELA 771

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BLAST of jgi.p|Trimin1|176367 vs. uniprot
Match: A0A7S1UH40_9STRA (Hypothetical protein n=1 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1UH40_9STRA)

HSP 1 Score: 573 bits (1477), Expect = 9.470e-195
Identity = 330/603 (54.73%), Postives = 421/603 (69.82%), Query Frame = 1

Query:   22 VTSLAALGADSLIFLAATVFLVPLSKKLNVSPVLGFLGAGLALGPHGFKLIRELRDLNALGELGITFLLFEQGLELSLDRLKALGKYAFGLGTLQVLLCTLAFGAFPFGEQSSVQLMESIFKSPDSLVNISRLDESVVIGAALSLSSSAFVLKILQEKGLLSGRAGQATLGVLLLQDIAVVPLLVLLPIIENQQAGAGDVPLMAQAAVLGGIALKAVGGLGAILVFGRFLLRRLFDGVAATRSTEAFVALCLLVAIGIGEVTDAMGLSASLGAFVAGTLLAETNYRMQVEADIAPFRGLLLALFFMTTGASVDPHMLWEQWPTVLGLLTGLLLVKGIITSTLG-PLFGLTKGESVRVGSILSGGGEFAFVVLTLAQKLNVLPLALDKLLVGTVVLSMALTPVLAAAGDKLGDYFDTQAGHSAPGAGFDDETLASPIGPFEGQDLVVICGFGPVGQIVASLISAPAVSGALCGARFVAFDLDPRRVSAARARGYPVFYGDGSQPKVIETAGIADPRALVVTYSDHALALSAVERLRGAYPRAKVFARATDYGQYWRLRREGATAVVSDLSEISIRLGSELL-ESFGVARTYEIQSAKAELRETL 1824
            V  L  LG D L+FLA TV ++PL+K L +S VLGFL  G  LGP GF + R+L+DL+ LGE+GI FLLFEQGLEL+L+RL++L K+AFGLG LQV+LCT+AFG FPF     V+L+E +F S  +LV+I+R DE+++IG+ALSLSSSAFVLK+LQEK L + R G A+LG+LLLQD+AVVPLL L+P+IE+Q  G G V    +   LG  AL  +G LG +L  G  +LRR+F  VA TRS+EAFVA+ LLVA+G+GE TD +GLS++LGAF AG LLAETNY+ Q+EADI PFRGL+L LFFM TGA+VDP ++   +PT LGLL GL+L K  I S LG  LFGL K E+VRVG++LSGGGEF+FVV TLA+KL VLP+ L KLL   VV SMALTPVL   GDK+G         S    G  +E+L       + +  VV+CGFGPVGQ VASL ++P +     G  FVAFD DP RV+ +R RG P +YGD SQ  V++ AGI +PR  VV +S+    L AV RLR  YP   +F+RA+   Q   L++ GA+ VV +  E+SIRLG+ ++ E FG   T   + AK  LR +L
Sbjct:   91 VDGLRILGNDGLVFLATTVLVIPLTKYLRISNVLGFLAMGFVLGPRGFGIFRDLQDLDVLGEIGILFLLFEQGLELTLERLRSLAKFAFGLGLLQVILCTIAFGVFPF--TGGVELLEVVFHSRPALVSITRPDEALIIGSALSLSSSAFVLKVLQEKNLQNARTGAASLGILLLQDLAVVPLLALIPVIESQLYGTGTVAGFDE---LGRTALVELGALGGVLAIGSLVLRRVFALVANTRSSEAFVAMTLLVALGMGEFTDKLGLSSTLGAFAAGALLAETNYKAQIEADIQPFRGLMLGLFFMVTGATVDPFVVMRDFPTFLGLLGGLILFKATIISALGYGLFGLEKEEAVRVGALLSGGGEFSFVVFTLAEKLEVLPVPLAKLLTAVVVASMALTPVLDFCGDKVGQLLRD----SPEEMGELEESLLLQNSEPDTRSPVVLCGFGPVGQTVASLFASPDLQEVDGGIPFVAFDKDPARVTESRQRGAPCYYGDASQRSVLDAAGILEPRGFVVVHSEFEERLGAVRRLRDQYPETPIFSRASRLNQQLLLKQSGASYVVPERDELSIRLGAAVIDELFGAVSTDAKEVAKRRLRASL 684

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BLAST of jgi.p|Trimin1|176367 vs. uniprot
Match: A0A7R9U8T7_9STRA (Hypothetical protein n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9U8T7_9STRA)

HSP 1 Score: 551 bits (1421), Expect = 1.290e-185
Identity = 329/616 (53.41%), Postives = 422/616 (68.51%), Query Frame = 1

Query:    1 MTGLEGEVTSLAALGADSLIFLAATVFLVPLSKKLNVSPVLGFLGAGLALGPHGFKLIRELRDLNALGELGITFLLFEQGLELSLDRLKALGKYAFGLGTLQVLLCTLAFGAFPFGEQSSVQLMESIFKSPDSLVNISRLDESVVIGAALSLSSSAFVLKILQEKGLLSGRAGQATLGVLLLQDIAVVPLLVLLPIIENQQAGAGDVPLMAQAAVLGGIALKAVGGLGAILVFGRFLLRRLFDGVAATRSTEAFVALCLLVAIGIGEVTDAMGLSASLGAFVAGTLLAETNYRMQVEADIAPFRGLLLALFFMTTGASVDPHMLWEQWPTVLGLLTGLLLVKGIITSTLGPL-FGLTKGESVRVGSILSGGGEFAFVVLTLAQKLNVLPLALDKLLVGTVVLSMALTPVLAAAGDKLGDYF-----DTQAGHSAPGAGFDDETLASPIGPFEGQDLVVICGFGPVGQIVASLISAPAVSGALCGA-RFVAFDLDPRRVSAARARGYPVFYGDGSQPKVIETAGIADPRALVVTYSDHALALSAVERLRGAYPRAKVFARATDYGQYWRLRREGATAVVSDLSEISIRLGSELLES-FGVARTYEIQSAKAELRETL 1824
            +T    + + L  LG+D LIFLAATV +VPL+K + VS VLGFL AG  LGP G  + ++L DL +L E+GI FLLFEQGLEL+LDRL+ L KYAFGLG LQ+LLCT+AFG FPF     V L+E +F+SP  LV I+R DE+++IG+ALSLSSSAFVLK+LQEKGL + R G A+LG+LLLQD+AVVP+LVL+PIIE Q +G G+V   A+   L   A  ++G L  +L  G  +LRR+FD +A+TRST+AFVA+ LLV +G+G+VT  +G+S +LGAF AG LLA+TNY+ QVEADI PFRGLLL LFFMTTGASVDP ++  Q PT L LL GL++ K  I S  G   F L K E++RVG++LSGGGEF+FVVL LA+KL VLP  L KLL   VV SMALTPVL  AG++LG+       D +   + P     D  L SPI         V+ GFGPVGQ V SL ++P +   + G   F AFD DP RV+ +R  G P FYGDGSQ  V++ AG+ DPRA VV +SD    L AV RLR A+P   +F+RA+   Q  +L+  GAT VV +  E+S+RLG+ ++E  +G      +++AK  LR  L
Sbjct:   60 LTRTYADPSGLGPLGSDGLIFLAATVIVVPLTKSVKVSSVLGFLFAGFLLGPAGIGVFKDLNDLESLSEIGILFLLFEQGLELTLDRLRNLAKYAFGLGFLQMLLCTIAFGLFPF--MRGVDLLEVVFRSPPDLVGITRPDEALIIGSALSLSSSAFVLKVLQEKGLQNTRTGAASLGILLLQDLAVVPILVLIPIIEAQLSGTGNVLSTAE---LAQSAAASLGALTLVLGGGSLILRRVFDMIASTRSTDAFVAMSLLVGLGMGKVTSMLGMSDTLGAFAAGALLADTNYKAQVEADIQPFRGLLLGLFFMTTGASVDPTVVAAQVPTFLALLGGLIVFKASIISLTGYRHFDLRKEEAIRVGALLSGGGEFSFVVLALAEKLGVLPSPLAKLLTAVVVASMALTPVLDFAGERLGELARDKNEDAEEFFNVPCG---DGCLVSPI---------VVLGFGPVGQTVVSLFASPELDEWIDGGIPFAAFDRDPARVAESRRLGVPCFYGDGSQQHVLQAAGVDDPRAFVVVHSDFGQRLEAVRRLRDAFPDKPIFSRASLLEQQVQLQAVGATYVVPERDELSLRLGAAVIEEVYGGVNADTLETAKKRLRAEL 658

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BLAST of jgi.p|Trimin1|176367 vs. uniprot
Match: A0A7S0UF61_HEMAN (Hypothetical protein n=1 Tax=Hemiselmis andersenii TaxID=464988 RepID=A0A7S0UF61_HEMAN)

HSP 1 Score: 551 bits (1421), Expect = 2.170e-184
Identity = 322/628 (51.27%), Postives = 421/628 (67.04%), Query Frame = 1

Query:   37 ALGADSLIFLAATVFLVPLSKKLNVSPVLGFLGAGLALGPHGFKLIRELRDLNALGELGITFLLFEQGLELSLDRLKALGKYAFGLGTLQVLLCTLAFGAFPFGEQSSVQLMESIFKSPDSLVNISRLDESVVIGAALSLSSSAFVLKILQEKGLLSGRAGQATLGVLLLQDIAVVPLLVLLPIIENQQAGAGDVPLMAQAAV--LGGIALKAVGGLGAILVFGRFLLRRLFDGVAATRSTEAFVALCLLVAIGIGEVTDAMGLSASLGAFVAGTLLAETNYRMQVEADIAPFRGLLLALFFMTTGASVDPHMLWEQWPTVLGLLTGLLLVKGIITSTLGPLFGLTKGESVRVGSILSGGGEFAFVVLTLAQKLNVLPLALDKLLVGTVVLSMALTPVLAAAGDKLGDYFDTQ-----------AGHSAPGAGFDDETLA--------SPIGPFEGQDLVVICGFGPVGQIVASLISAPAVSGALCGARFVAFDLDPRRVSAARARGYPVFYGDGSQPKVIETAGIADPRALVVTYSDHALALSAVERLRGAYPRAKVFARATDYGQYWRLRREGATAVVSDLSEISIRLGSELLESFGVARTYEIQSAKAELRETLACLSQVRQKKL 1857
            ALG D+++FLAATV +VP+ KKL++SPVLGFL AG+ LGP+GFK                 FLLFEQGLEL++DRL++L K+AFGLGTLQV L T AF A PF   S  Q++ES+F +   LV+I +LDE+ VIGAAL+LSSSAFVLKILQEKG L    G+A LGVLLLQDIAVVPLLVLLPI+E Q     +V   A+ A   LG +   A+GG+ A+L FG  +L ++F+ VA  RS+E F+ALCLL     G  T  +GLS +LGAF+AG LLAETNYR QVEADI PFRG+LL LFF+TTGASVD  +L ++WPT LG+L GL+ +K  IT  L   FGL+KG++VR G +LSGGGEFAFVVL LA+ L VLPL   KL+ G VV+SMALTP L++ GD   +Y DT+                P     D TLA        + I      D+VVICGFGP+GQ V SL+S+  +        +VAFDL+P RV     RG  VFYGDGSQP V+  AG+ +PR + VTY+D A  +S+V+RL+ A+P   + +R     + ++L   GAT V+ +LSE+S+RLGS +L+ FGV+    ++ + A+LR+ LA  ++++QK++
Sbjct:   14 ALGQDAIVFLAATVAVVPICKKLDLSPVLGFLAAGVVLGPNGFKXXXXXXXXXXXXXXXXLFLLFEQGLELTVDRLQSLFKFAFGLGTLQVALTTAAFTAVPFLGGS--QILESVFGASQDLVSIKQLDEAFVIGAALALSSSAFVLKILQEKGQLGSPTGRAALGVLLLQDIAVVPLLVLLPIVELQN----EVATAAEGASQDLGTLFGTAIGGVTALLAFGYIVLPKIFNAVAGARSSETFIALCLLTVAAAGTFTKTIGLSDTLGAFIAGILLAETNYRTQVEADIQPFRGILLGLFFVTTGASVDLPLLVQEWPTALGVLIGLISIKAAITGGLATNFGLSKGDAVRTGLLLSGGGEFAFVVLNLAKNLKVLPLDQTKLISGVVVVSMALTPALSSLGDAAAEYLDTEELKKKGRRRRRVPRGVPSVQVGDATLAGGDASDGDTEIEKMSMDDVVVICGFGPIGQTVGSLLSSQYLKDESLRIDYVAFDLNPDRVEEGSKRGIKVFYGDGSQPNVLRAAGVTNPRVICVTYTDLASRISSVQRLKEAFPGTPILSRGGVLSEVFQLEAAGATTVIPELSEVSLRLGSAVLDEFGVS-IKTMEDSMADLRDKLAARARIQQKRI 634

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BLAST of jgi.p|Trimin1|176367 vs. uniprot
Match: A0A834SJX9_9FABA (K(+) efflux antiporter 3, chloroplastic isoform X1 n=1 Tax=Senna tora TaxID=362788 RepID=A0A834SJX9_9FABA)

HSP 1 Score: 532 bits (1371), Expect = 1.190e-177
Identity = 304/592 (51.35%), Postives = 401/592 (67.74%), Query Frame = 1

Query:   16 GEVTSLAALGADSLIFLAATVFLVPLSKKLNVSPVLGFLGAGLALGPHGFKLIRELRDLNALGELGITFLLFEQGLELSLDRLKALGKYAFGLGTLQVLLCTLAFGAFPFGEQSSV--QLMESIFKSPDSLVNISRLDESVVIGAALSLSSSAFVLKILQEKGLLSGRAGQATLGVLLLQDIAVVPLLVLLPIIENQQAGAGDV-PLMAQAAVLGGIALKAVGGLGAILVFGRFLLRRLFDGVAATRSTEAFVALCLLVAIGIGEVTDAMGLSASLGAFVAGTLLAETNYRMQVEADIAPFRGLLLALFFMTTGASVDPHMLWEQWPTVLGLLTGLLLVKGIITSTLGPLFGLTKGESVRVGSILSGGGEFAFVVLTLAQKLNVLPLALDKLLVGTVVLSMALTPVLAAAGDKLGDYFDTQAGHSAPGAGFDDETLASPIGPFEGQDLVVICGFGPVGQIVASLISAPAVSGA---LCGARFVAFDLDPRRVSAARARGYPVFYGDGSQPKVIETAGIADPRALVVTYSDHALALSAVERLRGAYPRAKVFARATDYGQYWRLRREGATAVVSDLSEISIRLGSELLESFGV 1773
            G +  +  LG D+L FLA TV +VP  K +  SP+LGF  AG+ L   G  LIR + D+  L E GI FLLFE GLELSLDRLKAL KYAFG+G  QVL  TLAF AF      ++  +++E +F S   LVNI  +DE++VIGAALSLSSSAFVL++L EKG L  R G ATLG+LLLQDIAVVPLLV+LPI+E+Q    G + P + Q +      LKA+GGLG + + G+++LRR+F+ VA  RSTEAFVALCLL   G   VT  +G S +LGAF+AG +LAETN+R Q+EADI PF+GLLL LFF+TTG S+D  +L+ +WP VL LL GL+++K +I + +GP  GLT  ESVR+G +LS GGEF FVV +LA  L VLPL L+KLL+  VVLSMALTP+L  AG +   + D +          D E  AS +  F   + VVI GFG +GQ++A+ +S P  SG      G  +VAFDLDP  V AA   G+PV YGDGS+P V+++AGI+ P+A++V Y+   +A+ AV+RLR A+P   ++ARA D      L++ GAT  + + +E S++LGS+LL+  G+
Sbjct:  108 GAIEVMNDLGVDTLTFLAVTVLIVPTFKLMKASPILGFFFAGVVLNQFG--LIRNIEDVKVLSEWGILFLLFEMGLELSLDRLKALAKYAFGMGLAQVLFSTLAFTAFELPPNGAIGTKILEFLFHSRPDLVNIRSIDEAIVIGAALSLSSSAFVLQLLAEKGELPTRFGSATLGILLLQDIAVVPLLVILPILESQNLAEGSIWPTLVQES------LKALGGLGLLSLGGKYILRRVFEVVADARSTEAFVALCLLTIAGTSLVTQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFKGLLLGLFFLTTGTSIDMELLYREWPNVLSLLAGLIVIKTLIITAIGPRVGLTLRESVRIGLLLSQGGEFGFVVFSLANGLGVLPLELNKLLIIVVVLSMALTPLLNEAGRRAAGFIDGK---------IDSENKASEMADFNASEPVVILGFGQMGQVLANFLSNPLASGGDSDAVGWPYVAFDLDPTVVKAAGNLGFPVLYGDGSRPSVLQSAGISSPKAIMVMYAGKEMAIEAVQRLRFAFPAIPIYARAQDLKHLLDLKKAGATDAILENAETSLQLGSKLLQGLGM 682

[Close]

BLAST of jgi.p|Trimin1|176367 vs. uniprot
Match: B9RX36_RICCO (Glutathione-regulated potassium-efflux system protein kefB, putative n=4 Tax=Ricinus communis TaxID=3988 RepID=B9RX36_RICCO)

HSP 1 Score: 531 bits (1367), Expect = 2.010e-177
Identity = 304/584 (52.05%), Postives = 404/584 (69.18%), Query Frame = 1

Query:   40 LGADSLIFLAATVFLVPLSKKLNVSPVLGFLGAGLALGPHGFKLIRELRDLNALGELGITFLLFEQGLELSLDRLKALGKYAFGLGTLQVLLCTLAFGAFPFGEQSSV--QLMESIFKSPDSLVNISRLDESVVIGAALSLSSSAFVLKILQEKGLLSGRAGQATLGVLLLQDIAVVPLLVLLPIIENQQAGAGDV-PLMAQAAVLGGIALKAVGGLGAILVFGRFLLRRLFDGVAATRSTEAFVALCLLVAIGIGEVTDAMGLSASLGAFVAGTLLAETNYRMQVEADIAPFRGLLLALFFMTTGASVDPHMLWEQWPTVLGLLTGLLLVKGIITSTLGPLFGLTKGESVRVGSILSGGGEFAFVVLTLAQKLNVLPLALDKLLVGTVVLSMALTPVLAAAGDKLGDYFDTQAGHSAPGAGFDDETLASPIGPFEGQDLVVICGFGPVGQIVASLISAPAVSGA---LCGARFVAFDLDPRRVSAARARGYPVFYGDGSQPKVIETAGIADPRALVVTYSDHALALSAVERLRGAYPRAKVFARATDYGQYWRLRREGATAVVSDLSEISIRLGSELLESFGV 1773
            LG D+L FLA TV +VP+ K L  SP+LGF  AG+ L   G  LIR L D+  L E GI FLLFE GLELSL RLKAL K+AFG+G  QV+L TLAF AF      ++  +++E +F S   LVNI  +DE+VVIGAALSLSSSAFVL++L EKG L  R G ATLG+LLLQDIAVVPLLV+LP++E+Q      + P++A+ +      LKA+GGLG + + G+++LRR+F+ VA TRS+EAF+ALCLL   G    T  +G S +LGAF+AG LLAETN+R Q+EADI PFRGLLL LFF+TTG S+D  +L+ +WP VL LL GL+++K +I S +GP  GLT  ESVR+G +LS GGEFAFVV +LA +L VLPL L+KLL+  VVLSMALTP+L   G +  D+ D +         FD E  A+ +  F+G + V+I GFG +GQ++A+ +SAP  SG    L G  +VAFDL+P  V A+R  G+PV YGDGS+P V++TAGI+ P+A ++ ++     + AV+RLR A+P   ++ARA D      L++ GAT  + + +E S++LGS LL+  GV
Sbjct:   70 LGMDTLTFLAVTVVVVPVFKILRASPILGFFFAGVVLNQFG--LIRNLTDVKVLSEWGILFLLFEMGLELSLARLKALAKFAFGMGLTQVILSTLAFTAFELPPNGAIGTRILEFLFHSRSDLVNIRSIDEAVVIGAALSLSSSAFVLQLLAEKGELPTRFGSATLGILLLQDIAVVPLLVILPVLESQNLIEESIWPMLAKES------LKALGGLGLLSLGGKYILRRVFEVVAETRSSEAFIALCLLTVTGTSLSTQMLGFSDTLGAFLAGALLAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMQLLFREWPNVLSLLAGLIVIKTLIISAIGPRVGLTIRESVRIGFLLSQGGEFAFVVFSLANRLGVLPLELNKLLIIVVVLSMALTPLLNEVGRRAADFIDDK---------FDKEDKAAELVNFDGSEPVIILGFGQMGQVLANFLSAPLASGIDADLAGWPYVAFDLNPSVVKASRRLGFPVLYGDGSRPAVLQTAGISSPKAFMIMHTGKKRTIEAVQRLRLAFPGIPIYARAQDLVHLLDLKKAGATDAILENAETSLQLGSRLLKGLGV 636

[Close]

BLAST of jgi.p|Trimin1|176367 vs. uniprot
Match: A0A0D3E864_BRAOL (RCK N-terminal domain-containing protein n=14 Tax=Brassica TaxID=3705 RepID=A0A0D3E864_BRAOL)

HSP 1 Score: 528 bits (1360), Expect = 3.390e-176
Identity = 299/584 (51.20%), Postives = 401/584 (68.66%), Query Frame = 1

Query:   40 LGADSLIFLAATVFLVPLSKKLNVSPVLGFLGAGLALGPHGFKLIRELRDLNALGELGITFLLFEQGLELSLDRLKALGKYAFGLGTLQVLLCTLAFGAFPFGEQSSV--QLMESIFKSPDSLVNISRLDESVVIGAALSLSSSAFVLKILQEKGLLSGRAGQATLGVLLLQDIAVVPLLVLLPIIENQQ-AGAGDVPLMAQAAVLGGIALKAVGGLGAILVFGRFLLRRLFDGVAATRSTEAFVALCLLVAIGIGEVTDAMGLSASLGAFVAGTLLAETNYRMQVEADIAPFRGLLLALFFMTTGASVDPHMLWEQWPTVLGLLTGLLLVKGIITSTLGPLFGLTKGESVRVGSILSGGGEFAFVVLTLAQKLNVLPLALDKLLVGTVVLSMALTPVLAAAGDKLGDYFDTQAGHSAPGAGFDDETLASPIGPFEGQDLVVICGFGPVGQIVASLISAPAVSGA---LCGARFVAFDLDPRRVSAARARGYPVFYGDGSQPKVIETAGIADPRALVVTYSDHALALSAVERLRGAYPRAKVFARATDYGQYWRLRREGATAVVSDLSEISIRLGSELLESFGV 1773
            LG D+L FL  TV +VP  + L  SP+LGF  AG+ L   GF  IR L D+  L E GI FLLFE GLELSL RLKAL K+AFG+G  QVLLCTLAF AF      ++  +++E +F S   LVNI  +DE++VIGAALSLSSSAFVL++L EKG L  R G ATLG+LLLQDIAVVPLLV+LP++E+Q   G   +P++A+ +       KA+GGLG + + G+FLLRR+F+ VA TRS+EAFVALCLL   G   +T  +G S +LGAF+AG LLAETN+R Q+EADI PFRGLLL LFF+TTG S+D  +L+ +WP VL LL GL+++K +I + LGP  GLT  ESVR+G +LS GGEFAFVV +LA +L VLPL L+KLL+  VVLSMALTP L   G +  D+ D + G+       +DE +      F+  + +VI GFG +GQ++A+ +S P VSG    L G  ++ FDL+P  V  +R +G+P+ YGDGS+P V+++ G++ P+A+++ Y        AVERLR A+P  K+++RA D      L++ GAT  + + +E S++LGS++L  FGV
Sbjct:  105 LGFDTLTFLMVTVIIVPAFRVLKASPILGFFFAGVVLNQFGF--IRNLTDVKVLSEWGILFLLFEMGLELSLARLKALAKFAFGMGLSQVLLCTLAFTAFELPPNGAIGTRILEFLFHSRPDLVNIRSIDEAIVIGAALSLSSSAFVLQLLAEKGELPTRFGSATLGILLLQDIAVVPLLVVLPVLESQNLVGESILPMLAKESA------KALGGLGILSLGGKFLLRRIFEVVAETRSSEAFVALCLLTVAGTSLLTQKLGFSDTLGAFLAGALLAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMEVLFREWPNVLTLLAGLIVIKTLIITALGPRVGLTLQESVRIGFLLSQGGEFAFVVFSLANRLGVLPLELNKLLIIVVVLSMALTPTLNQLGRRAADFIDEKIGNEEN----EDEEVN-----FDVSESIVIIGFGQMGQVLANFLSTPLVSGVDSDLVGWPYIGFDLNPAVVKESRKQGFPILYGDGSRPSVLQSVGVSSPKAIMIMYKGKKRTTEAVERLRLAFPATKIYSRAQDLPHLLELKKAGATDAILENAETSLQLGSKMLRGFGV 671

[Close]

BLAST of jgi.p|Trimin1|176367 vs. uniprot
Match: A0A2C9V4K5_MANES (RCK N-terminal domain-containing protein n=2 Tax=Crotonoideae TaxID=235631 RepID=A0A2C9V4K5_MANES)

HSP 1 Score: 527 bits (1357), Expect = 2.840e-175
Identity = 300/583 (51.46%), Postives = 402/583 (68.95%), Query Frame = 1

Query:   40 LGADSLIFLAATVFLVPLSKKLNVSPVLGFLGAGLALGPHGFKLIRELRDLNALGELGITFLLFEQGLELSLDRLKALGKYAFGLGTLQVLLCTLAFGAFPFGEQSSV--QLMESIFKSPDSLVNISRLDESVVIGAALSLSSSAFVLKILQEKGLLSGRAGQATLGVLLLQDIAVVPLLVLLPIIENQQAGAGDV-PLMAQAAVLGGIALKAVGGLGAILVFGRFLLRRLFDGVAATRSTEAFVALCLLVAIGIGEVTDAMGLSASLGAFVAGTLLAETNYRMQVEADIAPFRGLLLALFFMTTGASVDPHMLWEQWPTVLGLLTGLLLVKGIITSTLGPLFGLTKGESVRVGSILSGGGEFAFVVLTLAQKLNVLPLALDKLLVGTVVLSMALTPVLAAAGDKLGDYFDTQAGHSAPGAGFDDETLASPIGPFEGQDLVVICGFGPVGQIVASLISAPAVSGALCGA--RFVAFDLDPRRVSAARARGYPVFYGDGSQPKVIETAGIADPRALVVTYSDHALALSAVERLRGAYPRAKVFARATDYGQYWRLRREGATAVVSDLSEISIRLGSELLESFGV 1773
            LG D+L FLA TV +VP+ K +  SP+LGF  AG+ L   GF  IR L D+  L E GI FLLFE GLELSL RLKAL K+AFG+G  QV+L TLAF AF      ++  +++E +F S   LVNI  +DE+VVIGAALSLSSSAFVL++L EKG L  R G ATLG+LLLQDIAVVPLLV+LP++E+Q    G + P++A+ +      LKA+GGLG + + G+++LRR+F+ VA  RS+EAFVALCLL   G   +T  +G S +LGAF+AG LLAETN+R Q+EADI PFRGLLL LFF+TTG S+D  +L+ +WP VL LL GL+++K +I ST+GP  GLT  ESVR+G +LS GGEFAFVV +LA +L VLPL L+KLL+  VVLSMALTP L  AG +  D+ D +         FD++  A  +  F+  + VVI GFG +GQ++A+ +S    +G        +VAFDL+P  V A+R  G+P  YGDGS+P V++TAGI+ P+A ++ Y+     + AV+RLR A+P   ++ARA D      L++ GAT  + + +E S++LGS+LL+S G+
Sbjct:  118 LGLDTLTFLAVTVVVVPVFKIIRASPILGFFFAGIVLNQFGF--IRNLTDVKVLSEWGILFLLFEMGLELSLARLKALAKFAFGMGLTQVVLSTLAFTAFELPPNGAIGTRILEFLFHSRSDLVNIRSIDEAVVIGAALSLSSSAFVLQLLAEKGELPTRFGSATLGILLLQDIAVVPLLVILPVLESQNLVEGSIWPMLAKES------LKALGGLGLLSLGGKYILRRVFEVVAEARSSEAFVALCLLTVSGTSLITQKLGFSDTLGAFLAGALLAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMQLLFREWPNVLSLLAGLIIIKTLIISTIGPRVGLTMRESVRIGFLLSQGGEFAFVVFSLANRLGVLPLELNKLLIIVVVLSMALTPFLNEAGRRAADFIDVK---------FDEDKTAEMVN-FDASEPVVILGFGQMGQVLANFLSVQLATGVDAEVVWPYVAFDLNPSVVKASRKLGFPTLYGDGSRPAVLQTAGISSPKAFMIMYTGRKRTIDAVQRLRLAFPAIPIYARAEDLMHLLDLKKAGATDAILENAETSLQLGSKLLKSLGI 682

The following BLAST results are available for this feature:

BLAST of jgi.p|Trimin1|176367 vs. uniprot

BLAST of jgi.p|Trimin1|176367 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25


Match Name	E-value	Identity	Description
A0A835ZEK7_9STRA	0.000e+0	100.00	Sodium/hydrogen exchanger family-domain-containing...	[more]
A0A835ZF36_9STRA	6.860e-309	77.49	K(+) efflux antiporter 3 n=1 Tax=Tribonema minus T...	[more]
A0A6H5JAW2_9PHAE	4.910e-233	62.94	RCK N-terminal domain-containing protein n=1 Tax=E...	[more]
A0A7S1UH40_9STRA	9.470e-195	54.73	Hypothetical protein n=1 Tax=Phaeomonas parva TaxI...	[more]
A0A7R9U8T7_9STRA	1.290e-185	53.41	Hypothetical protein n=1 Tax=Pinguiococcus pyrenoi...	[more]
A0A7S0UF61_HEMAN	2.170e-184	51.27	Hypothetical protein n=1 Tax=Hemiselmis andersenii...	[more]
A0A834SJX9_9FABA	1.190e-177	51.35	K(+) efflux antiporter 3, chloroplastic isoform X1...	[more]
B9RX36_RICCO	2.010e-177	52.05	Glutathione-regulated potassium-efflux system prot...	[more]
A0A0D3E864_BRAOL	3.390e-176	51.20	RCK N-terminal domain-containing protein n=14 Tax=...	[more]
A0A2C9V4K5_MANES	2.840e-175	51.46	RCK N-terminal domain-containing protein n=2 Tax=C...	[more]

Pages

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
Contig_137	contig	Contig_137:271113..277417 -

Analyses

This mRNA is derived from or has results from the following analyses


Analysis Name	Date Performed
Diamond blastx: OGS1.0 vs UniRef90	2022-09-19
OGS1.0 of Tribonema minus UTEX_B_3156	2021-06-17

Properties


Property Name	Value
ProteomeId	Trimin1\|176367\|e_gw1.137.35.1
GOs	GO:0003674,GO:0005215,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0006810,GO:0006811,GO:0006812,GO:0006996,GO:0008150,GO:0008324,GO:0009314,GO:0009416,GO:0009507,GO:0009534,GO:0009535,GO:0009536,GO:0009579,GO:0009628,GO:0009642,GO:0009643,GO:0009657,GO:0009658,GO:0009893,GO:0009987,GO:0010109,GO:0015075,GO:0015318,GO:0016020,GO:0016043,GO:0019222,GO:0022857,GO:0022890,GO:0031323,GO:0031325,GO:0031976,GO:0031984,GO:0034220,GO:0034357,GO:0042651,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044422,GO:0044424,GO:0044434,GO:0044435,GO:0044436,GO:0044444,GO:0044446,GO:0044464,GO:0048518,GO:0048522,GO:0050789,GO:0050794,GO:0050896,GO:0051179,GO:0051234,GO:0055035,GO:0055085,GO:0065007,GO:0071840,GO:0098655,GO:1905157
Description	efflux antiporter 3
COG category	P
Max annot lvl	35493\|Streptophyta
EggNOG OGs	COG0229@1\|root,COG0475@1\|root,KOG0856@2759\|Eukaryota,KOG1650@2759\|Eukaryota,37J86@33090\|Viridiplantae,3GEMI@35493\|Streptophyta,3HPZF@3699\|Brassicales
Evalue	1.4e-177
Seed ortholog	3711.Bra029519.1-P
Hectar predicted targeting category	other localisation
TranscriptId	176689
Track	FilteredModels1
ProteinId	176367

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Species	Type	Position
jgi.p\|Trimin1\|176367	gene_13816	Tribonema minus UTEX_B_3156	gene	Contig_137 271113..277417 -

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Species	Type	Position
jgi.p\|Trimin1\|176367	Trimin1\|176367\|e_gw1.137.35.1	Tribonema minus UTEX_B_3156	polypeptide	Contig_137 271113..277417 -

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
exon_13816_7	exon_13816_7	Tribonema minus UTEX_B_3156	exon	Contig_137 271113..271788 -
exon_13816_6	exon_13816_6	Tribonema minus UTEX_B_3156	exon	Contig_137 273401..273581 -
exon_13816_5	exon_13816_5	Tribonema minus UTEX_B_3156	exon	Contig_137 274112..274235 -
exon_13816_4	exon_13816_4	Tribonema minus UTEX_B_3156	exon	Contig_137 275336..275611 -
exon_13816_3	exon_13816_3	Tribonema minus UTEX_B_3156	exon	Contig_137 275951..276107 -
exon_13816_2	exon_13816_2	Tribonema minus UTEX_B_3156	exon	Contig_137 276428..276521 -
exon_13816_1	exon_13816_1	Tribonema minus UTEX_B_3156	exon	Contig_137 277060..277417 -

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
CDS_13816	CDS_13816	Tribonema minus UTEX_B_3156	CDS	Contig_137 277060..277417 -

Sequences

The following sequences are available for this feature:

mRNA - spliced transcript sequence
protein sequence
mRNA from alignment at Contig_137:271113..277417-
coding sequence from alignment at Contig_137:271113..277417-

spliced messenger RNA

>mRNA_13816 ID=mRNA_13816|Name=jgi.p|Trimin1|176367|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=1866bp|location=Sequence derived from alignment at Contig_137:271113..277417- (Tribonema minus UTEX_B_3156 )|Notes=Excludes all bases but those of type(s): exon.  
ATGACAGGATTGGAGGGGGAGGTGACGTCCCTGGCTGCCTTGGGCGCAGA
CTCCCTGATATTTCTAGCAGCCACTGTTTTTCTTGTCCCTTTGTCCAAGA
AGCTGAACGTCAGCCCTGTCCTCGGTTTCCTGGGAGCGGGCCTGGCGCTC
GGGCCGCACGGCTTCAAGCTCATCCGGGAGCTGAGAGATCTTAACGCTCT
CGGCGAATTGGGTATCACGTTTCTGCTTTTTGAACAGGGCCTGGAGCTCA
GCTTGGATCGGCTCAAGGCGCTTGGCAAGTACGCGTTCGGGCTGGGGACG
CTGCAGGTGCTGCTGTGCACATTGGCATTTGGAGCATTTCCATTTGGTGA
GCAATCTAGTGTGCAGCTGATGGAGTCGATCTTCAAGTCGCCAGATTCCC
TGGTCAACATCAGCCGGCTGGACGAATCTGTGGTCATTGGCGCAGCTCTC
AGCCTCAGCAGCAGCGCCTTCGTCCTGAAGATCCTTCAGGAGAAGGGGCT
GCTCAGCGGTCGAGCGGGCCAGGCCACCCTGGGCGTGCTGCTCCTCCAAG
ACATTGCCGTCGTCCCGCTTCTCGTCCTGCTGCCCATCATCGAGAACCAG
CAGGCGGGTGCGGGTGACGTGCCGCTCATGGCGCAGGCAGCAGTGCTCGG
CGGCATTGCGCTCAAAGCCGTCGGCGGCCTCGGCGCGATCCTCGTCTTTG
GTCGCTTCCTGCTGCGGCGGCTGTTCGATGGCGTCGCCGCTACGCGCAGC
ACCGAAGCCTTCGTCGCGCTGTGCCTGCTGGTGGCCATCGGCATTGGGGA
GGTTACGGATGCGATGGGTCTGAGTGCGTCGCTGGGGGCGTTCGTCGCGG
GGACGCTGCTCGCGGAGACCAACTACCGCATGCAGGTGGAGGCGGACATA
GCTCCATTCCGCGGCCTGCTTCTGGCGCTGTTCTTCATGACAACGGGGGC
CTCCGTAGACCCACACATGCTGTGGGAGCAGTGGCCCACTGTGCTGGGGC
TGCTCACAGGTTTACTGCTGGTCAAGGGCATCATCACCAGCACACTGGGC
CCCCTGTTTGGCCTGACAAAGGGGGAGTCGGTGCGCGTGGGGAGCATCTT
GAGCGGTGGCGGCGAGTTTGCATTCGTGGTGCTGACGCTCGCACAGAAGC
TCAATGTGCTGCCGCTCGCGCTGGACAAGCTCCTGGTGGGCACGGTGGTG
CTCTCCATGGCGCTGACCCCCGTCCTGGCCGCCGCCGGCGACAAACTCGG
CGACTACTTCGACACGCAAGCAGGGCACTCCGCACCCGGCGCGGGGTTCG
ACGACGAAACCCTGGCCTCCCCGATCGGCCCCTTCGAAGGCCAAGACCTA
GTTGTGATCTGCGGCTTTGGGCCCGTGGGGCAGATTGTGGCGAGCCTCAT
ATCGGCGCCCGCGGTGAGCGGCGCACTGTGCGGCGCGCGCTTCGTGGCCT
TTGACCTGGACCCGCGGCGCGTGAGCGCGGCGCGCGCGCGCGGCTACCCC
GTCTTCTACGGCGACGGCTCGCAGCCCAAGGTCATCGAGACGGCGGGGAT
CGCGGATCCGCGCGCGCTGGTAGTGACGTACTCGGACCACGCGCTAGCCC
TGAGCGCTGTGGAGAGGCTGCGGGGGGCGTACCCGCGCGCAAAAGTGTTC
GCGCGCGCGACCGACTACGGCCAGTACTGGCGGCTGCGGCGCGAGGGCGC
GACGGCGGTGGTGTCCGACCTCAGCGAGATCTCCATTCGGCTCGGGTCGG
AGCTGCTGGAGAGCTTCGGCGTCGCGCGCACGTATGAGATCCAGAGCGCC
AAGGCGGAGCTGCGCGAGACGCTCGCGTGCCTGTCGCAGGTGAGACAGAA
GAAACTTCAAAGTTGA

protein sequence of jgi.p|Trimin1|176367

>Trimin1|176367|e_gw1.137.35.1 ID=Trimin1|176367|e_gw1.137.35.1|Name=jgi.p|Trimin1|176367|organism=Tribonema minus UTEX_B_3156 |type=polypeptide|length=622bp
MTGLEGEVTSLAALGADSLIFLAATVFLVPLSKKLNVSPVLGFLGAGLAL
GPHGFKLIRELRDLNALGELGITFLLFEQGLELSLDRLKALGKYAFGLGT
LQVLLCTLAFGAFPFGEQSSVQLMESIFKSPDSLVNISRLDESVVIGAAL
SLSSSAFVLKILQEKGLLSGRAGQATLGVLLLQDIAVVPLLVLLPIIENQ
QAGAGDVPLMAQAAVLGGIALKAVGGLGAILVFGRFLLRRLFDGVAATRS
TEAFVALCLLVAIGIGEVTDAMGLSASLGAFVAGTLLAETNYRMQVEADI
APFRGLLLALFFMTTGASVDPHMLWEQWPTVLGLLTGLLLVKGIITSTLG
PLFGLTKGESVRVGSILSGGGEFAFVVLTLAQKLNVLPLALDKLLVGTVV
LSMALTPVLAAAGDKLGDYFDTQAGHSAPGAGFDDETLASPIGPFEGQDL
VVICGFGPVGQIVASLISAPAVSGALCGARFVAFDLDPRRVSAARARGYP
VFYGDGSQPKVIETAGIADPRALVVTYSDHALALSAVERLRGAYPRAKVF
ARATDYGQYWRLRREGATAVVSDLSEISIRLGSELLESFGVARTYEIQSA
KAELRETLACLSQVRQKKLQS*

mRNA from alignment at Contig_137:271113..277417-

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

>mRNA_13816 ID=mRNA_13816|Name=jgi.p|Trimin1|176367|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=6305bp|location=Sequence derived from alignment at Contig_137:271113..277417- (Tribonema minus UTEX_B_3156 )
ATGACAGGATTGGAGGGGGAGGTGACGTCCCTGGCTGCCTTGGGCGCAGA
CTCCCTGATATTTCTAGCAGCCACTGTTTTTCTTGTCCCTTTGTCCAAGA
AGCTGAACGTCAGCCCTGTCCTCGGTTTCCTGGGAGCGGGCCTGGCGCTC
GGGCCGCACGGCTTCAAGCTCATCCGGGAGCTGAGAGATCTTAACGCTCT
CGGCGAATTGGGTATCACGTTTCTGCTTTTTGAACAGGGCCTGGAGCTCA
GCTTGGATCGGCTCAAGGCGCTTGGCAAGTACGCGTTCGGGCTGGGGACG
CTGCAGGTGCTGCTGTGCACATTGGCATTTGGAGCATTTCCATTTGGTGA
GCAATCTAGTGTACAGCTGCTTGTTGTAAATCAATACAAGTACATAAGTA
CAACCCGATATAGTACAGCTCAATGTGCTGCTCTGAAGGCGGCGTGCACC
TGTCTGCTTTGCTTACTACTATCCGTACAGTAGTGCTGAATGCATTTTGT
GAATAAAACTGACTTCTAAGTACACTTGGCGAAGCCTTCGCCATGCCACA
ATCCAGACGCAACATCTCTTCTGCGTTACAAAGGAATTGACCGATCCGTC
TTTATCATCATTGACAGTCAACCTTGTGTTGACATGAGAACGTGACATGT
GCATCTGACTGACCGTAGTCATAATGTGCATTGCAGTGAATCCAGTACCA
CTTCTCTGATCAAATTGCATTGATGGCTTTTTCAGGCACTGGATGACGGC
GCTGCACAGCGGACAATTGAGTACTTCGAAGTATGTTGTTATTGCCAGCA
TCCACAACAGCTTAAGAGACTCATGCAGTTATCATGGTGCCTGCTGCTTC
CAACTTCTTTTTCCTTGTTTGCACTATTGAATTGCTGCAGTGGGAGGTGT
GCAGCTGATGGAGTCGATCTTCAAGTCGCCAGATTCCCTGGTCAACATCA
GCCGGCTGGACGAATCTGTGGTCATTGGCGCAGCTCTCAGGTGACTCTAA
CTTCTAGTTGAAGTGTCATTTGCTGCTCTTGCTTTTAAGAACCCTGTGGA
CACAATGACTCATGCGCAGCCAAGGCACGGTCCATGTGTTGCACCATATA
TGGTATGTAGCGGGCGTTCCTTTCTGCAGCACCCTTTTACCTACAGTAGC
TCTTTTATCCTGTGCCGGAACCCTCTTCATTGAAGAGTCTTCAACCTCAT
TTGTGCCTTTCTTTGCACCTTGACATGGCACACCGAATCAAAAATACAGT
AGTTTTTCATTTGACCCCCTGCCCCCCCCGCCCCCTCCCTGCCCCCCCCC
CTCCCTGCAGCCTCAGCAGCAGCGCCTTCGTCCTGAAGATCCTTCAGGAG
AAGGGGCTGCTCAGCGGTCGAGCGGGCCAGGCCACCCTGGGCGTGCTGCT
CCTCCAAGACATTGCCGTCGTCCCGCTTCTCGTCCTGCTGCCCATCATCG
AGAACCAGCAGGCGGGTGTGACGTGCCGCTCATCCCCCACTTCAACTCGC
TCCGATCGCGTGCGATGCGTGTGTGTATGTGTGTGTGTGTGTGTGTGTGT
GTGTGCGCGCGCGTGCGCCGTAATCCTCGTTGTGATACCCCGTTGCCCCT
CCCCCCGCGCCCGCCACCCACATGTGTGTATTACTACGCTCTCACAGCAG
TACGGTGTGGCCTCACAACTCACCATGCTTCTTGTCAAACCTCTTGTGCA
CCCCCATCACCCCCCCCCCCGCAGCAGTATAGTGTGGCCTCTGAACACAG
TTCTGATCTCTTCGTTTTGACCCCCGCCCCCCCCCCCCCCCCCCACACAC
ACACAGGCGGGTGACGTGCCGCTCATGGCGCAGGCAGCAGTGCTCGGCGG
CATTGCGCTCAAAGCCGTCGGCGGCCTCGGCGCGATCCTCGTCTTTGGTC
GCTTCCTGCTGCGGCGGCTGTTCGATGGCGTCGCCGCTACGCGCAGCACC
GAAGCCTTCGTCGCGCTGTGCCTGCTGGTGGCCATCGGCATTGGGGAGGT
TACGGATGCGATGGGTCTGAGTGCGTCGCTGGGGGCGTTCGTCGCGGGGA
CGCTGCTCGCGGAGACCAACTACCGCATGCAGGTGAGGCGTAGGGATGCG
AACGCGTGTACATGCATGTTAAGGCATATACAACACGCTTCGAAGTATAC
GTATCTTAAGGCGTGTAACTGTATACGCCGCCATCTGCGTTTCCTACCTC
GTAGTGAGTCGCAGGGACGCTGCTCGCGGAGATCAACTACCGCATGCAGG
TGAGGCGTAGAAATGCTGAAGTCCGTACGTGTATGGTAAGGCGTATACGA
CGACATGCTTCGCGAAGTATACGGATTTTATGATGTATAACTGCAGGCTG
TATACGCCGGCGTCTACGTTTTGTGAGTCGTGGGGACAGTGCTGGCGGAG
ACCAACAACCGCATGCAGGTGAGGTGTAGGGATGCGGAAGTACATGCATG
TACATTACAGCGTACAAAATCCAGATATATACTCAAGTTATATATAGAAG
TACAAACACTGTGTTGGGCTGCTAGCTGGAGCTGGCCTACCGAACACGTG
GAAGCTTTTCATACTCAACACCCATGCCTCGAACGCTAAAAATCTTGCAC
ACAGAAGTCAGAAGTCTTTTCACTACTGTGGATATGGTGGCACCGTTTGA
CTCGTGGCCCATTGCCGCAGCAGCGGCGACCACAGTCATTGATGAAAGGC
ATTCAGTTTGGAGGCTAACCACACATTGTCACCTGGAGCTCTCCGATCTT
TTGATTCGTTTCCGTATATACTTCAACATGTTTATATTTTGGCGTATATG
TGCGGGCTGTATATGCCGCCATCTACGTTTCCTGCCTGCAGCTCATCCTG
CTGCGATCTTGCATGCGCGTTTGCAAGCTCCCGTCGTAGTGATTCGAGGG
ACGCTGCTTGCAGAGACCAACTACGGCATGCAGGTGGGGGGGGGAGGGGG
GGGGGGGTTTGAGCGGCAAAGCGAGGGAGCCACCAATTGTTGGTAGCTGC
AGCTGTTGGTTGCCGGAACGAAATCCAGCAGCAGCAGCAGCAGCAGCAGC
AGTGCGCAAAACGTCCATTGATCTTATCATCCACATGCCAGCGGCAGTGC
AGACTTTGACATACCAGCATGATGCCCATAAAGCGTTATTTAGATTATGA
TACTGATATGCTGGTGCATTTGCTGTTGGCAGGTGGAGGCGGACATAGCT
CCATTCCGCGGCCTGCTTCTGGCGCTGTTCTTCATGACAACGGGGGCCTC
CGTAGACCCACACATGCTGTGGGAGCAGTGGCCCACTGTGCTGGGGCTGC
TCACAGGTGACTCACGACTCATATTTGTGACTCATGAGTCATGAGTACTC
TAGCCCTGAAGGAATGTGCGGGACAACCTCAGTTCAGGTGTTGTTGGCAT
CATGCACCAGGTGACTCGCGACTCATGTTTGTGACTCATGAGTCATGAGT
AATCCAGCCCTGAAGAGATTCAAGGGACACTCTGCCTCATGTCAGATGTT
GTTGGCATCATGGCATGCGCCATGTCCTTGGAGACACCTGCAGCGCTGAG
CAACATGTAAATCTAACCCTAACCCTAAGAGAAACGTGACACAATGAGAT
GCGACACATGCTTCTACTTGCTCTTAAACATGTAAGTTATTTTGCAATGT
AACCTGACATTACATTACACGTTGCTAGCGGGACGCGCTGCCGCAGGTTT
ACTGCTGATGAAGGGCATCATCACGAGTACATTGTCATCGTGGCATGGCG
TGCGCACACATCCACTTCTTCTGCATTATACTTCCAGTATATCCTGACAT
CACAATACACGTTGCCACTGGTATGCGCTGCCAAAGGTTTACTGCTGGTC
AAGGGCATCATCACCAGCACACTGGGCCCCCTGTTTGGCCTGACAAAGGG
GGAGTCGGTGCGCGTGGGGAGCATCTTGAGCGGTGGCGGCGAGTTTGCAT
TCGTGGTGCTGACGCTCGCACAGAAGCTCAATGTGCTGCCGCTCGCGCTG
GACAAGCTCCTGGTGGGGTAAGTGTAACTTGAAGTTCATCGTTGAATAGA
AATTAACACTATTCAACGAAGACACAACCTTTTGAACAGTGCTGATGTGG
TAAGGGTATTCCTCAGCAATGCTATAGAACATAACAGCGAAGCACAGCGT
TGGCGTAGCAAGTGAACACTAAGTTATCCAGCATTGGTCTCCACCTTCCT
GTGTGGTGGGGGCTTATTGGTAGGCACCGCGATGCGCAACCTCGCATTTG
CGGTGCCACCACTACCAACGTTTCTGGCACTTGCGGTGCGACCCCACTGG
CACCAAGGGGGTCTAGACCCCCCTTGCTGGCACAAAAGTAATGACTGATA
ATGTGCTGCCCAGCTCCTCCCTGGAAACGCGGTGGGGTATGTATGTAGGC
TGAATTTCCTCTAATGGCTGTCTTTGCATTTTAGGAGCTCACACTAGCAT
AGAAACTGAACATACCGCTCTTGGTGGTGCTTAAAAGCGACGTTCATTTC
ATTCAAGGGTGAAGTGTGTCTTGACTCTGGAGTTTGCAGCTGTGGCTCAT
CCTATGAGCACGATGTGATACCCTGGCGGCAATGAACAATCTGCTAGTGC
TGTCTGCTAGTACTGTGAGCCCAGTATGCAGTCAGGGCTATGGCTTTGGG
TTGTGCCATGTGGTCTTGACAACATTGTTGCAAAGGACATGTGCTGCTCC
TTCTACAAACTTGAATGATTGAAGGCACCAGACTATCTGCAGCTGGTGCG
TAGTTTGCAGCACGGCCAGAAATTGAGGTTGCACTATGTAGTGTTAGAGT
TGTTGTTAGAGAACGAGATGTGATGAATCTACTACGTCACATGAGTGAAG
ACACAAATTTAGCCTTTCTGCAGCTGGTGCAGACTTGTTTGACGAATGTA
TCAGACTGAGATTGAGCGCATTTGCTCAAATTGTCACCACATTCTGTCAG
AGCATCAGTGCTACTGCTTTCTCTCTTTGTGGTGTAGATTGCATGCATCT
GCTCAAATTGTCACCACATTCTGTCAGAGCATCAGTGCTACTGCTTTCGC
TCTTTCTGGTTTGTACTAATGCATGCAGCGCCAGGCTAAACTAAACCCTT
TTTCTGTTTGTTCTGGTTTTTCATGTTTGTTGTTGTGACCGCCCTGAGAA
CGGGCCATCATGTTCAGCTGTGCATGACCGCTTAAAGCTAAAAGCTTGTC
CTGACCCCAACCCTACATGACGGTTTTACCAGAGAGGTGCTGGCGGACCA
TCAAATCTGCTGCCGACAGCAACTTTGCGATCACGCTTTGTCGAGACGTG
CGTTCGTATTGAAGTAGCATAAGTGTGTACTACATTTCACGTGCACAATC
CCTCATCCTGTGTTGCTGCTTGCGTCTTTGAAAGCAACCATGCCAACCAC
GCGCTGATGCGTCACGCAGTGGCCACTGCAGACCGCGCAGGGGTCCCCCC
CCTGCCAGGCTTTAAACAGAGCACTGACCCGCTGCTCACACTCAACCCCC
CCCCTACCCCCCCCTCCTCAGCACGGTGGTGCTCTCCATGGCGCTCACCC
CCGTCCTGGTGCCTCAGCCACCTCCACCATGCCCTAAACTCCTCATGCCC
CCCCCCCCCCCACCTCCCCCTCTCCTCAGCACGGTGGTGCTCTCCATGGC
GCTGACCCCCGTCCTGGCCGCCGCCGGCGACAAACTCGGCGACTACTTCG
ACACGCAAGCAGGGCACTCCGCACCCGGCGCGGGGTTCGACGACGAAACC
CTGGCCTCCCCGATCGGCCCCTTCGAAGGCCAAGACCTAGTTGTGATCTG
CGGCTTTGGGCCCGTGGGGCAGATTGTGGCGAGCCTCATATCGGCGCCCG
CGGTGAGCGGCGCACTGTGCGGCGCGCGCTTCGTGGCCTTTGACCTGGAC
CCGCGGCGCGTGAGCGCGGCGCGCGCGCGCGGCTACCCCGTCTTCTACGG
CGACGGCTCGCAGCCCAAGGTCATCGAGACGGCGGGGATCGCGGATCCGC
GCGCGCTGGTAGTGACGTACTCGGACCACGCGCTAGCCCTGAGCGCTGTG
GAGAGGCTGCGGGGGGCGTACCCGCGCGCAAAAGTGTTCGCGCGCGCGAC
CGACTACGGCCAGTACTGGCGGCTGCGGCGCGAGGGCGCGACGGCGGTGG
TGTCCGACCTCAGCGAGATCTCCATTCGGCTCGGGTCGGAGCTGCTGGAG
AGCTTCGGCGTCGCGCGCACGTATGAGATCCAGAGCGCCAAGGCGGAGCT
GCGCGAGACGCTCGCGTGCCTGTCGCAGGTGAGACAGAAGAAACTTCAAA
GTTGA

Coding sequence (CDS) from alignment at Contig_137:271113..277417-

>mRNA_13816 ID=mRNA_13816|Name=jgi.p|Trimin1|176367|organism=Tribonema minus UTEX_B_3156 |type=CDS|length=1866bp|location=Sequence derived from alignment at Contig_137:271113..277417- (Tribonema minus UTEX_B_3156 )
ATGACAGGATTGGAGGGGGAGGTGACGTCCCTGGCTGCCTTGGGCGCAGA
CTCCCTGATATTTCTAGCAGCCACTGTTTTTCTTGTCCCTTTGTCCAAGA
AGCTGAACGTCAGCCCTGTCCTCGGTTTCCTGGGAGCGGGCCTGGCGCTC
GGGCCGCACGGCTTCAAGCTCATCCGGGAGCTGAGAGATCTTAACGCTCT
CGGCGAATTGGGTATCACGTTTCTGCTTTTTGAACAGGGCCTGGAGCTCA
GCTTGGATCGGCTCAAGGCGCTTGGCAAGTACGCGTTCGGGCTGGGGACG
CTGCAGGTGCTGCTGTGCACATTGGCATTTGGAGCATTTCCATTTGGTGA
GCAATCTAGTGTGCAGCTGATGGAGTCGATCTTCAAGTCGCCAGATTCCC
TGGTCAACATCAGCCGGCTGGACGAATCTGTGGTCATTGGCGCAGCTCTC
AGCCTCAGCAGCAGCGCCTTCGTCCTGAAGATCCTTCAGGAGAAGGGGCT
GCTCAGCGGTCGAGCGGGCCAGGCCACCCTGGGCGTGCTGCTCCTCCAAG
ACATTGCCGTCGTCCCGCTTCTCGTCCTGCTGCCCATCATCGAGAACCAG
CAGGCGGGTGCGGGTGACGTGCCGCTCATGGCGCAGGCAGCAGTGCTCGG
CGGCATTGCGCTCAAAGCCGTCGGCGGCCTCGGCGCGATCCTCGTCTTTG
GTCGCTTCCTGCTGCGGCGGCTGTTCGATGGCGTCGCCGCTACGCGCAGC
ACCGAAGCCTTCGTCGCGCTGTGCCTGCTGGTGGCCATCGGCATTGGGGA
GGTTACGGATGCGATGGGTCTGAGTGCGTCGCTGGGGGCGTTCGTCGCGG
GGACGCTGCTCGCGGAGACCAACTACCGCATGCAGGTGGAGGCGGACATA
GCTCCATTCCGCGGCCTGCTTCTGGCGCTGTTCTTCATGACAACGGGGGC
CTCCGTAGACCCACACATGCTGTGGGAGCAGTGGCCCACTGTGCTGGGGC
TGCTCACAGGTTTACTGCTGGTCAAGGGCATCATCACCAGCACACTGGGC
CCCCTGTTTGGCCTGACAAAGGGGGAGTCGGTGCGCGTGGGGAGCATCTT
GAGCGGTGGCGGCGAGTTTGCATTCGTGGTGCTGACGCTCGCACAGAAGC
TCAATGTGCTGCCGCTCGCGCTGGACAAGCTCCTGGTGGGCACGGTGGTG
CTCTCCATGGCGCTGACCCCCGTCCTGGCCGCCGCCGGCGACAAACTCGG
CGACTACTTCGACACGCAAGCAGGGCACTCCGCACCCGGCGCGGGGTTCG
ACGACGAAACCCTGGCCTCCCCGATCGGCCCCTTCGAAGGCCAAGACCTA
GTTGTGATCTGCGGCTTTGGGCCCGTGGGGCAGATTGTGGCGAGCCTCAT
ATCGGCGCCCGCGGTGAGCGGCGCACTGTGCGGCGCGCGCTTCGTGGCCT
TTGACCTGGACCCGCGGCGCGTGAGCGCGGCGCGCGCGCGCGGCTACCCC
GTCTTCTACGGCGACGGCTCGCAGCCCAAGGTCATCGAGACGGCGGGGAT
CGCGGATCCGCGCGCGCTGGTAGTGACGTACTCGGACCACGCGCTAGCCC
TGAGCGCTGTGGAGAGGCTGCGGGGGGCGTACCCGCGCGCAAAAGTGTTC
GCGCGCGCGACCGACTACGGCCAGTACTGGCGGCTGCGGCGCGAGGGCGC
GACGGCGGTGGTGTCCGACCTCAGCGAGATCTCCATTCGGCTCGGGTCGG
AGCTGCTGGAGAGCTTCGGCGTCGCGCGCACGTATGAGATCCAGAGCGCC
AAGGCGGAGCTGCGCGAGACGCTCGCGTGCCTGTCGCAGGTGAGACAGAA
GAAACTTCAAAGTTGA