mRNA_12737 (mRNA) Tribonema minus UTEX_B_3156

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
Namejgi.p|Trimin1|173668
Unique NamemRNA_12737
TypemRNA
OrganismTribonema minus UTEX_B_3156 (Tribonema minus UTEX_B_3156 )
Homology
BLAST of jgi.p|Trimin1|173668 vs. uniprot
Match: A0A836CP06_9STRA (Batten's disease protein Cln3 n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CP06_9STRA)

HSP 1 Score: 277 bits (708), Expect = 2.200e-94
Identity = 136/136 (100.00%), Postives = 136/136 (100.00%), Query Frame = 1
Query:    1 MCTQVAGAKEINSGGVGLVFLCDVAPGFFVKLSAPYWFHLVPHSTRVIACGVLMVCAFSLVGCGTTLGAQLAGVSLSSTQSALGETTFLSLAARYDSRWALTMWSSGTGFAGVFGYVTYVFCHLFGETQSVALSLW 408
            MCTQVAGAKEINSGGVGLVFLCDVAPGFFVKLSAPYWFHLVPHSTRVIACGVLMVCAFSLVGCGTTLGAQLAGVSLSSTQSALGETTFLSLAARYDSRWALTMWSSGTGFAGVFGYVTYVFCHLFGETQSVALSLW
Sbjct:    1 MCTQVAGAKEINSGGVGLVFLCDVAPGFFVKLSAPYWFHLVPHSTRVIACGVLMVCAFSLVGCGTTLGAQLAGVSLSSTQSALGETTFLSLAARYDSRWALTMWSSGTGFAGVFGYVTYVFCHLFGETQSVALSLW 136          
BLAST of jgi.p|Trimin1|173668 vs. uniprot
Match: W7TQX4_9STRA (Cln3like protein n=4 Tax=Monodopsidaceae TaxID=425072 RepID=W7TQX4_9STRA)

HSP 1 Score: 135 bits (339), Expect = 8.330e-35
Identity = 74/137 (54.01%), Postives = 84/137 (61.31%), Query Frame = 1
Query:   10 QVAGAKEINSGGVGLVFLCDVAPGFFVKLSAPYWFHLVPHSTRVIACGVLMVCAFSLVGCGTTLGAQLAGVSLSSTQSALGETTFLSLAARYDSRWALTMWSSGTGFAGVFGYVTYVFCHL-----FGETQSVALSL 405
             +AGAKEIN+GGVGLVFL DVAP F VKLSAPYWFH V +S R   CG LM  +   V  G TL  QL GV   S Q+ +GE + L L A+Y     +T WSSGTGFAG+ GY   VF HL     F  T   AL L
Sbjct:   41 MLAGAKEINAGGVGLVFLADVAPTFLVKLSAPYWFHHVSYSLRASLCGFLMAASLLTVALGRTLAVQLTGVGFCSLQAGIGEASMLGLTAQYHGPSTITAWSSGTGFAGILGYAWVVFFHLLLHMSFPATLIAALLL 177          
BLAST of jgi.p|Trimin1|173668 vs. uniprot
Match: A0A2P6VN99_9CHLO (Battenin n=1 Tax=Micractinium conductrix TaxID=554055 RepID=A0A2P6VN99_9CHLO)

HSP 1 Score: 122 bits (307), Expect = 2.150e-30
Identity = 63/132 (47.73%), Postives = 82/132 (62.12%), Query Frame = 1
Query:   10 QVAGAKEINSGGVGLVFLCDVAPGFFVKLSAPYWFHLVPHSTRVIACGVLMVCAFSLVGCGTTLGAQLAGVSLSSTQSALGETTFLSLAARYDSRWALTMWSSGTGFAGVFGYVTYVFCHLFGETQSVALSL 405
             +AGA E+++  VGLV+LC VAP    K SAPYWFHLV +STRV    +LM C+++ V   +  G QL GV  +S Q  LGE + L++ + Y  R A++MWSSGTGFAGVFGY      H+ G     A  L
Sbjct:   28 MLAGANEVSAAAVGLVYLCAVAPAMLCKASAPYWFHLVRYSTRVHIVALLMSCSYTTVALSSARGWQLLGVVFASLQGGLGEASCLAMTSYYSGRPAISMWSSGTGFAGVFGYAWVALLHMLGGLSFRATLL 159          
BLAST of jgi.p|Trimin1|173668 vs. uniprot
Match: A0A6H5JLK7_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JLK7_9PHAE)

HSP 1 Score: 121 bits (304), Expect = 9.180e-30
Identity = 63/112 (56.25%), Postives = 80/112 (71.43%), Query Frame = 1
Query:   10 QVAGAKEINSGGVGLVFLCDVAPGFFVKLSAPYWFHLVPHSTRVIACGVLMVCAFSLVGCGTTLGAQLAGVSLSSTQSALGETTFLSLAARYDSRWALTMWSSGTGFAGVFG 345
             +A AKEI    VG+VF  DVAP   +K++APYWFHLVP+S RV AC +L+V +   VG G +   QL GV  SS Q+ LGE++FL+LA+ YD+  ALT WSSGTGFAG+FG
Sbjct:   80 MMAVAKEIAPDAVGVVFFADVAPTMVIKVTAPYWFHLVPYSARVWACTMLLVLSLVTVGYGESTRVQLLGVCFSSLQAGLGESSFLALASFYDTPRALTAWSSGTGFAGIFG 191          
BLAST of jgi.p|Trimin1|173668 vs. uniprot
Match: A0A7S0XR50_9STRA (Hypothetical protein (Fragment) n=1 Tax=Chrysocystis fragilis TaxID=1411660 RepID=A0A7S0XR50_9STRA)

HSP 1 Score: 116 bits (290), Expect = 3.700e-29
Identity = 68/137 (49.64%), Postives = 92/137 (67.15%), Query Frame = 1
Query:   10 QVAGAKEINSGGVGLVFLCDVAPGFFVKLSAPYWFHLVPHSTRVIACGVLMVCAFSLVGCGTTLGAQLAGVSLSSTQSALGETTFLSLAARYDSRWA--LTMWSSGTGFAGVFGYVTYV-FCHLFGE--TQSVALSL 405
             +A AK I +GGV LV+L  + PG  VK S+P+WF +V + TR+      M  +F+LVG G++L AQLAGV+L S QSA+GE + L+L++RY  R A  +  WSSGTGFAGVFGY   V F    G+  TQ+VAL++
Sbjct:   34 MIASAKSIANGGVALVYLAGIGPGVCVKSSSPFWFDMVGYRTRLGLATSCMAASFALVGFGSSLYAQLAGVALCSAQSAMGEASLLALSSRYPRRGADAVAAWSSGTGFAGVFGYAWVVAFGATLGDIGTQAVALAV 170          
BLAST of jgi.p|Trimin1|173668 vs. uniprot
Match: A0A5J4XD34_9CHLO (Golgi integral membrane protein n=1 Tax=Trebouxia sp. A1-2 TaxID=2608996 RepID=A0A5J4XD34_9CHLO)

HSP 1 Score: 119 bits (298), Expect = 5.700e-29
Identity = 64/133 (48.12%), Postives = 83/133 (62.41%), Query Frame = 1
Query:   10 QVAGAKEINSGGVGLVFLCDVAPGFFVKLSAPYWFHLVPHSTRVIACGVLMVCAFSLVGCGTTLGAQLAGVSLSSTQSALGETTFLSLAARYDSRWALTMWSSGTGFAGVFGYVTYVFCHLF-GETQSVALSL 405
             +AGA +I+SG VGLV+ C V P   VKLSAPYWFHL+ +  R+    V+M  ++  V  G  +  QL GV+ ++ Q  LGE + L+L + YDSR ALT+WSSGTGFAGV GY      HL+ G T    L L
Sbjct:   24 MIAGANDISSGAVGLVYFCAVFPALLVKLSAPYWFHLISYDVRMTVVAVMMTGSYLTVAYGRHMSWQLLGVTFAAFQGGLGEASTLALCSLYDSRRALTLWSSGTGFAGVAGYTWVAVLHLWLGFTFRTTLLL 156          
BLAST of jgi.p|Trimin1|173668 vs. uniprot
Match: A0A7S1JHF2_9EUGL (Hypothetical protein n=1 Tax=Eutreptiella gymnastica TaxID=73025 RepID=A0A7S1JHF2_9EUGL)

HSP 1 Score: 115 bits (289), Expect = 5.080e-28
Identity = 64/125 (51.20%), Postives = 83/125 (66.40%), Query Frame = 1
Query:   10 QVAGAKEINSGGVGLVFLCDVAPGFFVKLSAPYWFHLVPHSTRVIACGVLMVCAFSLVGCGT-TLGAQLA--GVSLSSTQSALGETTFLSLAARYDSRWALTMWSSGTGFAGVFGYVTYVFCHLF 375
             +AGAK+I    VGLV++C+V PGF +KL+ PYWFH V +  RV    +LM  +F +V  G  T  A L+  G+   S Q +LGE +F++L+A YDSR ALT WSSGTGFAGVFGY+   F  LF
Sbjct:   26 MIAGAKDILPSAVGLVYVCNVLPGFAIKLTGPYWFHYVRYKHRVYCASLLMGLSFLIVALGQITKNAALSLFGILFCSAQGSLGEASFVALSAFYDSRKALTAWSSGTGFAGVFGYLWVSFFTLF 150          
BLAST of jgi.p|Trimin1|173668 vs. uniprot
Match: A0A2P6TP97_CHLSO (Batten s disease Cln3 isoform B n=2 Tax=Chlorella sorokiniana TaxID=3076 RepID=A0A2P6TP97_CHLSO)

HSP 1 Score: 116 bits (290), Expect = 8.170e-28
Identity = 61/132 (46.21%), Postives = 80/132 (60.61%), Query Frame = 1
Query:   10 QVAGAKEINSGGVGLVFLCDVAPGFFVKLSAPYWFHLVPHSTRVIACGVLMVCAFSLVGCGTTLGAQLAGVSLSSTQSALGETTFLSLAARYDSRWALTMWSSGTGFAGVFGYVTYVFCHLFGETQSVALSL 405
             +AGA +I++  VGLV+L  V P    K S PYWFHLV + TR+ A  +LM  +++ V   ++ G QL GV L+S QS LGE + L+L + Y  R A+TMWSSGTGFAGVFGY   +  H        A  L
Sbjct:   32 MLAGANQISAAAVGLVYLAAVGPSLLCKASVPYWFHLVRYRTRMQAVALLMTASYTTVALSSSRGWQLLGVVLASLQSGLGEASCLALTSHYRGRAAITMWSSGTGFAGVFGYAWVLLLHFLAGLSFTATLL 163          
BLAST of jgi.p|Trimin1|173668 vs. uniprot
Match: A0A383VZS1_TETOB (Uncharacterized protein n=1 Tax=Tetradesmus obliquus TaxID=3088 RepID=A0A383VZS1_TETOB)

HSP 1 Score: 115 bits (289), Expect = 1.290e-27
Identity = 59/120 (49.17%), Postives = 75/120 (62.50%), Query Frame = 1
Query:   10 QVAGAKEINSGGVGLVFLCDVAPGFFVKLSAPYWFHLVPHSTRVIACGVLMVCAFSLVGCGTTLGAQLAGVSLSSTQSALGETTFLSLAARYDSRWALTMWSSGTGFAGVFGYVTYVFCH 369
             +AGA EI +  VGLV+L  + P   VKLSAPYWFHLV +STR      LM C+F +VGC  ++  QL GV L + Q  LGE + L L + Y ++ +LT WSSGTGFAG+ GY      H
Sbjct:   47 MIAGANEIAASAVGLVYLFAIGPTIIVKLSAPYWFHLVGYSTRFWVAAALMSCSFIIVGCSKSMTKQLLGVGLVAIQGGLGEASALGLCSLYPNQLSLTAWSSGTGFAGIAGYAWVAVLH 166          
BLAST of jgi.p|Trimin1|173668 vs. uniprot
Match: A0A7S0WK83_9CHLO (Hypothetical protein (Fragment) n=1 Tax=Pyramimonas obovata TaxID=1411642 RepID=A0A7S0WK83_9CHLO)

HSP 1 Score: 109 bits (273), Expect = 1.390e-27
Identity = 58/119 (48.74%), Postives = 78/119 (65.55%), Query Frame = 1
Query:   10 QVAGAKEINSGGVGLVFLCDVAPGFFVKLSAPYWFHLVPHSTRVIACGVLMVCAFSLVGCGTTLGA-------QLAGVSLSSTQSALGETTFLSLAARYDSRWALTMWSSGTGFAGVFG 345
             +AGAK+I+SGGV LV++  + PG  VK++ P W+HLV +  R  A  V MV +FS V  G+ L +       QL GV+  S QS LGE++ L+L+  Y  + A+TMWSSGTGFAGVFG
Sbjct:   68 MIAGAKDISSGGVALVYIASIFPGLAVKMTGPCWYHLVSYRLRAYAVAVAMVASFSCVAMGSVLRSPELKLPVQLLGVAFCSFQSGLGESSTLALSVHYSPQRAITMWSSGTGFAGVFG 186          
The following BLAST results are available for this feature:
BLAST of jgi.p|Trimin1|173668 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A836CP06_9STRA2.200e-94100.00Batten's disease protein Cln3 n=1 Tax=Tribonema mi... [more]
W7TQX4_9STRA8.330e-3554.01Cln3like protein n=4 Tax=Monodopsidaceae TaxID=425... [more]
A0A2P6VN99_9CHLO2.150e-3047.73Battenin n=1 Tax=Micractinium conductrix TaxID=554... [more]
A0A6H5JLK7_9PHAE9.180e-3056.25Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A7S0XR50_9STRA3.700e-2949.64Hypothetical protein (Fragment) n=1 Tax=Chrysocyst... [more]
A0A5J4XD34_9CHLO5.700e-2948.12Golgi integral membrane protein n=1 Tax=Trebouxia ... [more]
A0A7S1JHF2_9EUGL5.080e-2851.20Hypothetical protein n=1 Tax=Eutreptiella gymnasti... [more]
A0A2P6TP97_CHLSO8.170e-2846.21Batten s disease Cln3 isoform B n=2 Tax=Chlorella ... [more]
A0A383VZS1_TETOB1.290e-2749.17Uncharacterized protein n=1 Tax=Tetradesmus obliqu... [more]
A0A7S0WK83_9CHLO1.390e-2748.74Hypothetical protein (Fragment) n=1 Tax=Pyramimona... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Contig_105contigContig_105:253257..255243 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Tribonema minus UTEX_B_31562021-06-17
Properties
Property NameValue
ProteomeIdTrimin1|173668|e_gw1.105.6.1
DescriptionCLN3 protein
COG categoryS
Max annot lvl121069|Pythiales
EggNOG OGsKOG3880@1|root,KOG3880@2759|Eukaryota,1MCIU@121069|Pythiales
Evalue1.93e-29
Seed ortholog36331.EPrPI00000019753
Hectar predicted targeting categoryother localisation
TranscriptId173990
TrackFilteredModels1
ProteinId173668
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameSpeciesTypePosition
jgi.p|Trimin1|173668gene_12737Tribonema minus UTEX_B_3156 geneContig_105 253257..255243 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
jgi.p|Trimin1|173668Trimin1|173668|e_gw1.105.6.1Tribonema minus UTEX_B_3156 polypeptideContig_105 253257..255243 -


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
exon_12737_2exon_12737_2Tribonema minus UTEX_B_3156 exonContig_105 253257..253491 -
exon_12737_1exon_12737_1Tribonema minus UTEX_B_3156 exonContig_105 255068..255243 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
CDS_12737CDS_12737Tribonema minus UTEX_B_3156 CDSContig_105 255068..255243 -


Sequences
The following sequences are available for this feature:

spliced messenger RNA

>mRNA_12737 ID=mRNA_12737|Name=jgi.p|Trimin1|173668|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=411bp|location=Sequence derived from alignment at Contig_105:253257..255243- (Tribonema minus UTEX_B_3156 )|Notes=Excludes all bases but those of type(s): exon.  
ATGTGTACGCAGGTGGCGGGGGCCAAGGAGATCAACTCGGGGGGGGTGGG
GCTCGTCTTTCTGTGCGACGTCGCCCCCGGCTTCTTTGTCAAGCTGTCGG
CGCCGTACTGGTTCCACCTGGTGCCGCACAGCACGCGCGTCATCGCGTGC
GGCGTGCTTATGGTGTGCGCATTCAGCCTCGTGGGCTGCGGCACGACACT
GGGCGCGCAGCTGGCGGGCGTGTCGCTCTCGAGCACGCAGAGCGCGCTGG
GCGAGACGACGTTCCTGTCGCTGGCGGCGCGCTACGACAGCCGCTGGGCG
CTCACCATGTGGAGCAGCGGCACGGGCTTCGCCGGCGTGTTCGGGTACGT
AACGTATGTATTTTGTCACCTTTTTGGGGAGACGCAGAGTGTTGCTCTTT
CGCTGTGGTGA
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protein sequence of jgi.p|Trimin1|173668

>Trimin1|173668|e_gw1.105.6.1 ID=Trimin1|173668|e_gw1.105.6.1|Name=jgi.p|Trimin1|173668|organism=Tribonema minus UTEX_B_3156 |type=polypeptide|length=137bp
MCTQVAGAKEINSGGVGLVFLCDVAPGFFVKLSAPYWFHLVPHSTRVIAC
GVLMVCAFSLVGCGTTLGAQLAGVSLSSTQSALGETTFLSLAARYDSRWA
LTMWSSGTGFAGVFGYVTYVFCHLFGETQSVALSLW*
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mRNA from alignment at Contig_105:253257..255243-

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_12737 ID=mRNA_12737|Name=jgi.p|Trimin1|173668|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=1987bp|location=Sequence derived from alignment at Contig_105:253257..255243- (Tribonema minus UTEX_B_3156 )
ATGTGTACGCAGGTGGCGGGGGCCAAGGAGATCAACTCGGGGGGGGTGGG GCTCGTCTTTCTGTGCGACGTCGCCCCCGGCTTCTTTGTCAAGCTGTCGG CGCCGTACTGGTTCCACCTGGTGCCGCACAGCACGCGCGTCATCGCGTGC GGCGTGCTTATGGTGTGCGCATTCAGGTGAGACGAGCCCCAACTGCAGCC CTGGTTGCAGCCTTTCGCCAAACCCTATGGACGGCTGAATTCTGGCTTAC GGCTCACGCTCAGGTGCAGGACACTGAGCTCAAGTTCTTGTGTTCTGGCG CCACCTGGTACTGCACAGTACGCGTGTCATCGCGTGCGGCGTGCTTATGG TGTGCGCATTCAGGTGAGACGAGCCCTAATTGCAGCCCTAGTTGGCCTTC TGCCAAACCCTACGAAGGGCTGAAGTCTGTGAATCCATGACTCCTGGCCC ATGCTCTCGTGTGGGGCGCATCGAGCTTAGATGTTCATGCACTGGTTCCA CGCGCCTCTGCTGCGCAGCATGCGCATCACAAGTGCAGCGCACTCATCGT GTGCGCTTCAGCTGAGGCAAGCCCTAGTTGCGGTTATTTGCCAAACCCTA AAAGTACAGCACTCCGTGCCATGCTGTAAACCACTGTATCCTGTGGGCGC TCGCGCAGTTGGCTCATGATCGTGCTCAACCCCTGACGTTGTGTGCAGTT TCATTCTCCCCGCGCTTATCGGCCCTAACCCTAACCCTAACCCTAAGATT GCTGCAGTACTGGTTTGTGATGCCTCATAACGCTAATACAACTACTGTTA CGGCTAATTTTACCCCTGAGATGCTGACACATGGCAGCATGACGCCCGGC TCGCGGCCGATGGGAGCATCACTCGTCGACCGCGCAAACTTCATGTTTTG AACCGCAACACCGCCCGTCAGCTCTGCTTGCATCCCCGCACAATGCATGG CAGCGCGGCGTACCCCGCCTGCAAGCACCAGGCACGCAACCACCAGTTAA GGGCAGCGTCAGTCACCTGTGTCTAGCCCTCAGTCATTGCAGGGCTAGGG CAGGGGCCAGATGTAGGGCATCGCCAACCGTGCAGGACATACAGTTATAA CCATTACTGTATACATACCCATTACCGCATCTGATCACTTCATGCTGCCG CCCGCTGCGCAATTGGTTACCTGCCGCACGACGCTCTGCACGCAGCTGCC GGGCCTGCCGCTCTAATCATCAGGGCGGGCTCATGTGCCTGTAGCCCCAA CCCTAGTTCATCACCATAACCGGAGACCGTAACCCTTTCTCAGCTGCCCC TACGCGCATAGCCTGATGGGCTGCGGCGTGACGCTTGGCGCGACGCCGTT TCTGCAACACTTTCCGCCAGCAACCTTGTACCCTAACTGCAAGGGCGAGA CGTTTCTGTAAGCTGAAGTCTAATGCGTAATCTCAACCTACCCTCTATAA ACTCCACCCTCGTCTGTAACTGCCACACGACGCTTCGCGTGCAGCTGGCG CGCATAGTGCGCTAACCGTCCGGGCGCGACGATGCTCGTATAAGCCTGAC CCTCACACGTTTTCATCGCAAGTCCCAGCCCCAATCCGTAACTGCCGTGC ATGCAGCCTGGTCGGCTGCGGCACGACGCTCGGCGAGCAGGTGGCGGGCG TATCGTTCTAACAATCGACGTGAGATGTGGCCGCAACCCTAGCCCTATGG TCGTAACCCTCACCCTAAGTCCTGAACCCTGATCCGTAACTGCCGCGCGC AGCCTCGTGGGCTGCGGCACGACACTGGGCGCGCAGCTGGCGGGCGTGTC GCTCTCGAGCACGCAGAGCGCGCTGGGCGAGACGACGTTCCTGTCGCTGG CGGCGCGCTACGACAGCCGCTGGGCGCTCACCATGTGGAGCAGCGGCACG GGCTTCGCCGGCGTGTTCGGGTACGTAACGTATGTATTTTGTCACCTTTT TGGGGAGACGCAGAGTGTTGCTCTTTCGCTGTGGTGA
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Coding sequence (CDS) from alignment at Contig_105:253257..255243-

>mRNA_12737 ID=mRNA_12737|Name=jgi.p|Trimin1|173668|organism=Tribonema minus UTEX_B_3156 |type=CDS|length=411bp|location=Sequence derived from alignment at Contig_105:253257..255243- (Tribonema minus UTEX_B_3156 )
ATGTGTACGCAGGTGGCGGGGGCCAAGGAGATCAACTCGGGGGGGGTGGG
GCTCGTCTTTCTGTGCGACGTCGCCCCCGGCTTCTTTGTCAAGCTGTCGG
CGCCGTACTGGTTCCACCTGGTGCCGCACAGCACGCGCGTCATCGCGTGC
GGCGTGCTTATGGTGTGCGCATTCAGCCTCGTGGGCTGCGGCACGACACT
GGGCGCGCAGCTGGCGGGCGTGTCGCTCTCGAGCACGCAGAGCGCGCTGG
GCGAGACGACGTTCCTGTCGCTGGCGGCGCGCTACGACAGCCGCTGGGCG
CTCACCATGTGGAGCAGCGGCACGGGCTTCGCCGGCGTGTTCGGGTACGT
AACGTATGTATTTTGTCACCTTTTTGGGGAGACGCAGAGTGTTGCTCTTT
CGCTGTGGTGA
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