mRNA_10827 (mRNA) Tribonema minus UTEX_B_3156

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
Namejgi.p|Trimin1|173293
Unique NamemRNA_10827
TypemRNA
OrganismTribonema minus UTEX_B_3156 (Tribonema minus UTEX_B_3156 )
Homology
BLAST of jgi.p|Trimin1|173293 vs. uniprot
Match: A0A835ZI30_9STRA (Kinase-like domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZI30_9STRA)

HSP 1 Score: 452 bits (1163), Expect = 1.460e-160
Identity = 233/233 (100.00%), Postives = 233/233 (100.00%), Query Frame = 1
Query:    1 HPASRTHAHVDVQVHMLQRLASIKNVIRLEGQGVTCDGHFLVTSPYGRRLKPTDSAHVMLRAVRDVASALAMMAAVDPPIIHRDISIGNIVVGSSSSSSEETSAYLIDFATALAETSQSSDPETITGTYIFMACQVMLTGVHSIKTDLESLFLVLLYLACDERVPWARAPSRRMSCALKIQALTMSHDTLFAQTRDDLLPAVTSLHELFKILPAGSQWRDSCSISAAARAVHG 699
            HPASRTHAHVDVQVHMLQRLASIKNVIRLEGQGVTCDGHFLVTSPYGRRLKPTDSAHVMLRAVRDVASALAMMAAVDPPIIHRDISIGNIVVGSSSSSSEETSAYLIDFATALAETSQSSDPETITGTYIFMACQVMLTGVHSIKTDLESLFLVLLYLACDERVPWARAPSRRMSCALKIQALTMSHDTLFAQTRDDLLPAVTSLHELFKILPAGSQWRDSCSISAAARAVHG
Sbjct:    1 HPASRTHAHVDVQVHMLQRLASIKNVIRLEGQGVTCDGHFLVTSPYGRRLKPTDSAHVMLRAVRDVASALAMMAAVDPPIIHRDISIGNIVVGSSSSSSEETSAYLIDFATALAETSQSSDPETITGTYIFMACQVMLTGVHSIKTDLESLFLVLLYLACDERVPWARAPSRRMSCALKIQALTMSHDTLFAQTRDDLLPAVTSLHELFKILPAGSQWRDSCSISAAARAVHG 233          
BLAST of jgi.p|Trimin1|173293 vs. uniprot
Match: A0A2P4NXJ1_RHIID (Non-specific serine/threonine protein kinase n=1 Tax=Rhizophagus irregularis (strain DAOM 181602 / DAOM 197198 / MUCL 43194) TaxID=747089 RepID=A0A2P4NXJ1_RHIID)

HSP 1 Score: 67.4 bits (163), Expect = 1.460e-9
Identity = 54/179 (30.17%), Postives = 88/179 (49.16%), Query Frame = 1
Query:   37 QVHMLQRLASIKNVIRLEGQGVTCDGH-FLVTSPYGRRLKPTD-SAHVMLRAVRDVASALAMMAAVDPPIIHRDISIGNIVVGSSSSSSEETSAYLIDFATALAETSQSSDPETITGTYIFMACQVM------LTGVHSIKTDLESLFLVLLYLACDERVPWARAPSRRMSCALKIQAL 549
            ++ ML+ L   + + ++  +G+  DG  F+VT+P G  L+         L+A+RDV  AL      +  I HRD+S  NIV        +    YLID+  A   +   + P  +TGTY+F +  V+      +   +S+  ++ESLF V +Y+ACD  V W +      S A+K   +
Sbjct:  115 ELKMLEILKDCEYIPQVINKGLLSDGRMFIVTTPAGVHLEAKKHGVKGTLKALRDVMKALKFSH--EHHIYHRDVSYLNIVY-------KNQRGYLIDWGVASNTSDLVNAP--LTGTYLFSSLPVLNCFESGVPHTYSVNDEIESLFYVFMYIACDGIVRWRKHSDLSTSVAIKYNVM 282          
BLAST of jgi.p|Trimin1|173293 vs. uniprot
Match: U9SS86_RHIID (Non-specific serine/threonine protein kinase n=1 Tax=Rhizophagus irregularis (strain DAOM 181602 / DAOM 197198 / MUCL 43194) TaxID=747089 RepID=U9SS86_RHIID)

HSP 1 Score: 67.4 bits (163), Expect = 1.510e-9
Identity = 54/179 (30.17%), Postives = 88/179 (49.16%), Query Frame = 1
Query:   37 QVHMLQRLASIKNVIRLEGQGVTCDGH-FLVTSPYGRRLKPTD-SAHVMLRAVRDVASALAMMAAVDPPIIHRDISIGNIVVGSSSSSSEETSAYLIDFATALAETSQSSDPETITGTYIFMACQVM------LTGVHSIKTDLESLFLVLLYLACDERVPWARAPSRRMSCALKIQAL 549
            ++ ML+ L   + + ++  +G+  DG  F+VT+P G  L+         L+A+RDV  AL      +  I HRD+S  NIV        +    YLID+  A   +   + P  +TGTY+F +  V+      +   +S+  ++ESLF V +Y+ACD  V W +      S A+K   +
Sbjct:  101 ELKMLEILKDCEYIPQVINKGLLSDGRMFIVTTPAGVHLEAKKHGVKGTLKALRDVMKALKFSH--EHHIYHRDVSYLNIVY-------KNQRGYLIDWGVASNTSDLVNAP--LTGTYLFSSLPVLNCFESGVPHTYSVNDEIESLFYVFMYIACDGIVRWRKHSDLSTSVAIKYNVM 268          
BLAST of jgi.p|Trimin1|173293 vs. uniprot
Match: A0A2Z6SRG1_9GLOM (Non-specific serine/threonine protein kinase n=2 Tax=Rhizophagus clarus TaxID=94130 RepID=A0A2Z6SRG1_9GLOM)

HSP 1 Score: 67.4 bits (163), Expect = 2.370e-9
Identity = 55/179 (30.73%), Postives = 91/179 (50.84%), Query Frame = 1
Query:   37 QVHMLQRLASIKNVIRLEGQGVTCDGH-FLVTSPYGRRLKPTD-SAHVMLRAVRDVASALAMMAAVDPPIIHRDISIGNIVVGSSSSSSEETSAYLIDFATALAETSQSSDPETITGTYIFMACQVML---TGV---HSIKTDLESLFLVLLYLACDERVPWARAPSRRMSCALKIQAL 549
            ++ +L+ L   + + ++  +G+  DG  F+VT+P G  L+         L+A+RDV  AL      +  I HRD+S  NIV        +    YLID+  A   +   + P  +TGTY+F +  V++   +GV   +S+  ++ESLF V +Y+ACD  V W +      S A+K   +
Sbjct:  319 ELKILEILKDCEYIPQVINKGLLSDGRMFIVTTPAGVHLEAKKHGVKGTLKALRDVMKALKFSH--EHHIYHRDVSYLNIVY-------KNQRGYLIDWGVASNTSDLVNAP--LTGTYLFSSLPVLMCFESGVPHTYSVNDEIESLFYVFMYIACDGVVRWRKHSDLSTSVAIKYNVM 486          
BLAST of jgi.p|Trimin1|173293 vs. uniprot
Match: A0A015JT29_RHIIW (Non-specific serine/threonine protein kinase n=7 Tax=Rhizophagus irregularis TaxID=588596 RepID=A0A015JT29_RHIIW)

HSP 1 Score: 67.4 bits (163), Expect = 2.400e-9
Identity = 54/179 (30.17%), Postives = 88/179 (49.16%), Query Frame = 1
Query:   37 QVHMLQRLASIKNVIRLEGQGVTCDGH-FLVTSPYGRRLKPTD-SAHVMLRAVRDVASALAMMAAVDPPIIHRDISIGNIVVGSSSSSSEETSAYLIDFATALAETSQSSDPETITGTYIFMACQVM------LTGVHSIKTDLESLFLVLLYLACDERVPWARAPSRRMSCALKIQAL 549
            ++ ML+ L   + + ++  +G+  DG  F+VT+P G  L+         L+A+RDV  AL      +  I HRD+S  NIV        +    YLID+  A   +   + P  +TGTY+F +  V+      +   +S+  ++ESLF V +Y+ACD  V W +      S A+K   +
Sbjct:  337 ELKMLEILKDCEYIPQVINKGLLSDGRMFIVTTPAGVHLEAKKHGVKGTLKALRDVMKALKFSH--EHHIYHRDVSYLNIVY-------KNQRGYLIDWGVASNTSDLVNAP--LTGTYLFSSLPVLNCFESGVPHTYSVNDEIESLFYVFMYIACDGIVRWRKHSDLSTSVAIKYNVM 504          
BLAST of jgi.p|Trimin1|173293 vs. uniprot
Match: A0A395MSV4_9HYPO (Non-specific serine/threonine protein kinase n=1 Tax=Fusarium flagelliforme TaxID=2675880 RepID=A0A395MSV4_9HYPO)

HSP 1 Score: 67.0 bits (162), Expect = 3.250e-9
Identity = 47/124 (37.90%), Postives = 69/124 (55.65%), Query Frame = 1
Query:  118 FLVTSPYGRRLKPTDSAHVMLRAVRDVASALAMMAAVDPPIIHRDISIGNIVVGSSSSSSEETSAYLIDFATALAETSQSSDPETITGTYIFMACQVMLTGVHSIKTDLESLFLVLLYLACDER 489
            + +TSPYGR +         L A+RD   A   +      I+HRDISI NI++ + + + EE+   LID   AL  T++ S  +TITGT  FMA  ++    ++ + DLES F VLL+ A  +R
Sbjct:  348 YTITSPYGRHIIEYTCIKEFLVALRDAIKAHRSLYTAGH-ILHRDISINNIII-TEAMTPEESHGTLIDLDAALDMTAEKSSQKTITGTKPFMAIGLLHGEENAYQHDLESFFYVLLFSATIDR 469          
BLAST of jgi.p|Trimin1|173293 vs. uniprot
Match: I1RDP0_GIBZE (EKC/KEOPS complex subunit BUD32 n=6 Tax=Fusarium sambucinum species complex TaxID=569360 RepID=I1RDP0_GIBZE)

HSP 1 Score: 65.9 bits (159), Expect = 8.030e-9
Identity = 45/124 (36.29%), Postives = 67/124 (54.03%), Query Frame = 1
Query:  118 FLVTSPYGRRLKPTDSAHVMLRAVRDVASALAMMAAVDPPIIHRDISIGNIVVGSSSSSSEETSAYLIDFATALAETSQSSDPETITGTYIFMACQVMLTGVHSIKTDLESLFLVLLYLACDER 489
            + +TSP+GR +    S    L  +RD   A   +   D  I+HRDISI NI++ + + S  E+S  LID   A+  T +    +TITGT  FMA  ++    ++ + DLES F V L+ A  +R
Sbjct:  355 YTITSPFGRHITQYSSIKEFLVVLRDAIKAHRSLYT-DGRILHRDISINNIII-TEAKSPGESSGTLIDLDAAVEMTDEKPTRKTITGTKPFMAIGLLQGEENAYRHDLESFFYVFLFSAAIDR 476          
BLAST of jgi.p|Trimin1|173293 vs. uniprot
Match: A0A2G9I0Y3_9LAMI (Serine/threonine protein kinase n=1 Tax=Handroanthus impetiginosus TaxID=429701 RepID=A0A2G9I0Y3_9LAMI)

HSP 1 Score: 60.8 bits (146), Expect = 4.600e-7
Identity = 52/145 (35.86%), Postives = 71/145 (48.97%), Query Frame = 1
Query:  178 LRAVRDVASALAMMAA-VDPPIIHRDISIGNIVVGSSSSSSEETSAYLIDFATALAETSQSSDPETITGTYIFMACQVMLTGVHSIKTDLESLFLVLLYLACDERVPWARAPSRRMSCALKIQALTMSHDTLFAQTRDDLLPAVT 609
            +R V+ VA ALA +     PPI+HRD+SI NI++ S      E    L DF TA   TS SS+  T+ G+Y +MA ++ L+   + K+D+ S  +V L       V   R P   +S  L   AL    DT      D  LP  T
Sbjct:  922 VRIVKGVAHALAYLHHDCSPPIVHRDVSINNILLES------ELEPRLSDFGTAKLLTSDSSNWTTVAGSYGYMAPELALSMKVTEKSDVYSFGVVAL------EVMMGRHPGELISSLLAKAALQNDPDTFLKDLLDQRLPLPT 1054          
BLAST of jgi.p|Trimin1|173293 vs. uniprot
Match: A0A2G9GP10_9LAMI (Serine/threonine protein kinase n=1 Tax=Handroanthus impetiginosus TaxID=429701 RepID=A0A2G9GP10_9LAMI)

HSP 1 Score: 58.9 bits (141), Expect = 2.020e-6
Identity = 52/145 (35.86%), Postives = 69/145 (47.59%), Query Frame = 1
Query:  178 LRAVRDVASALAMMAA-VDPPIIHRDISIGNIVVGSSSSSSEETSAYLIDFATALAETSQSSDPETITGTYIFMACQVMLTGVHSIKTDLESLFLVLLYLACDERVPWARAPSRRMSCALKIQALTMSHDTLFAQTRDDLLPAVT 609
            +R V+ VA ALA M     PPI+HRD+SI NI++ S      E    L DF TA   TS SS+  T+ G+Y +MA ++ LT   + K+D+ S  +V L       V   R P   +S      AL    D       D  LP  T
Sbjct:  839 VRIVKGVAHALAYMHHDCSPPIVHRDVSINNILLES------EFEPRLSDFGTAKLLTSDSSNWTTVAGSYGYMAPELALTMKATEKSDVYSFGVVAL------EVMMGRHPGELISSLSAKAALQNDPDMFLKDLLDQRLPLPT 971          
BLAST of jgi.p|Trimin1|173293 vs. uniprot
Match: A0A8J5TYC7_FUSOX (Uncharacterized protein n=1 Tax=Fusarium oxysporum f. sp. rapae TaxID=485398 RepID=A0A8J5TYC7_FUSOX)

HSP 1 Score: 57.4 bits (137), Expect = 5.300e-6
Identity = 40/128 (31.25%), Postives = 66/128 (51.56%), Query Frame = 1
Query:  121 LVTSPYGRRLKPTDSAHVMLRAVRDVASALAMMAAVDPPIIHRDISIGNIVVGSSSSSSEETSAY-LIDFATALAETSQSSDPETITGTYIFMACQVMLTG----VHSIKTDLESLFLVLLYLACDER 489
            +V +P+GR +    +   +L  +RD   A   +  +D  I+HRDIS+GNI++ ++ ++    S + LID   A     +   P  +TGT  FMA  +        +H+ + DLES F V L++A   R
Sbjct:  237 MVITPFGRPITEYSTVLEVLECMRDTIKAHRSLY-LDARILHRDISVGNILISNAGNNEPGASKWILIDLDCAKDMEFEPEKPYNLTGTKTFMAIDLSRGSDDRVLHTYRHDLESFFYVFLFMAISRR 363          
The following BLAST results are available for this feature:
BLAST of jgi.p|Trimin1|173293 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 24
Match NameE-valueIdentityDescription
A0A835ZI30_9STRA1.460e-160100.00Kinase-like domain-containing protein n=1 Tax=Trib... [more]
A0A2P4NXJ1_RHIID1.460e-930.17Non-specific serine/threonine protein kinase n=1 T... [more]
U9SS86_RHIID1.510e-930.17Non-specific serine/threonine protein kinase n=1 T... [more]
A0A2Z6SRG1_9GLOM2.370e-930.73Non-specific serine/threonine protein kinase n=2 T... [more]
A0A015JT29_RHIIW2.400e-930.17Non-specific serine/threonine protein kinase n=7 T... [more]
A0A395MSV4_9HYPO3.250e-937.90Non-specific serine/threonine protein kinase n=1 T... [more]
I1RDP0_GIBZE8.030e-936.29EKC/KEOPS complex subunit BUD32 n=6 Tax=Fusarium s... [more]
A0A2G9I0Y3_9LAMI4.600e-735.86Serine/threonine protein kinase n=1 Tax=Handroanth... [more]
A0A2G9GP10_9LAMI2.020e-635.86Serine/threonine protein kinase n=1 Tax=Handroanth... [more]
A0A8J5TYC7_FUSOX5.300e-631.25Uncharacterized protein n=1 Tax=Fusarium oxysporum... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Contig_101contigContig_101:494404..495105 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Tribonema minus UTEX_B_31562021-06-17
Properties
Property NameValue
ProteomeIdTrimin1|173293|e_gw1.101.10.1
BRITEko00000,ko04131
KEGG koko:K20476
DescriptionProtein kinase domain
COG categoryT
Max annot lvl2759|Eukaryota
EggNOG OGsKOG1164@1|root,KOG1164@2759|Eukaryota,3824U@33090|Viridiplantae
Evalue5.99e-09
Seed ortholog88036.EFJ35818
Hectar predicted targeting categoryother localisation
TranscriptId173615
TrackFilteredModels1
ProteinId173293
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameSpeciesTypePosition
jgi.p|Trimin1|173293gene_10827Tribonema minus UTEX_B_3156 geneContig_101 494404..495105 +


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
exon_10827_1exon_10827_1Tribonema minus UTEX_B_3156 exonContig_101 494404..495105 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
CDS_10827CDS_10827Tribonema minus UTEX_B_3156 CDSContig_101 494404..495105 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
jgi.p|Trimin1|173293Trimin1|173293|e_gw1.101.10.1Tribonema minus UTEX_B_3156 polypeptideContig_101 494404..495105 +


Sequences
The following sequences are available for this feature:

spliced messenger RNA

>mRNA_10827 ID=mRNA_10827|Name=jgi.p|Trimin1|173293|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=702bp|location=Sequence derived from alignment at Contig_101:494404..495105+ (Tribonema minus UTEX_B_3156 )|Notes=Excludes all bases but those of type(s): exon.  
CATCCCGCGTCACGCACTCACGCCCATGTTGATGTGCAGGTCCACATGTT
GCAACGCTTGGCGAGCATCAAGAATGTCATTCGCCTGGAGGGGCAGGGCG
TCACCTGTGACGGGCACTTTTTGGTGACTTCGCCATACGGCCGCCGTCTG
AAGCCCACAGACTCTGCGCACGTGATGCTGAGAGCAGTCCGTGACGTAGC
ATCAGCGCTCGCCATGATGGCAGCTGTTGACCCGCCAATCATCCACAGGG
ACATATCAATTGGCAACATTGTCGTTGGCAGCAGCAGCAGCAGCAGCGAG
GAGACGAGCGCGTACCTGATAGACTTTGCCACTGCGCTCGCCGAGACAAG
TCAGAGCTCTGACCCAGAGACCATCACTGGCACATACATCTTCATGGCTT
GTCAGGTCATGCTGACTGGGGTGCACAGTATCAAGACAGACCTCGAGAGT
CTATTCTTGGTGCTGCTATACCTGGCCTGTGATGAGCGGGTGCCCTGGGC
ACGCGCACCCAGTCGCAGGATGTCGTGTGCGCTGAAGATCCAGGCACTCA
CAATGTCGCATGACACTCTGTTTGCACAGACCCGTGATGATCTTCTCCCC
GCAGTCACTAGTCTCCACGAGCTGTTTAAGATCTTACCAGCAGGCAGCCA
ATGGCGTGACTCCTGCAGCATTTCTGCAGCGGCTCGAGCAGTGCATGGCT
GA
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protein sequence of jgi.p|Trimin1|173293

>Trimin1|173293|e_gw1.101.10.1 ID=Trimin1|173293|e_gw1.101.10.1|Name=jgi.p|Trimin1|173293|organism=Tribonema minus UTEX_B_3156 |type=polypeptide|length=234bp
HPASRTHAHVDVQVHMLQRLASIKNVIRLEGQGVTCDGHFLVTSPYGRRL
KPTDSAHVMLRAVRDVASALAMMAAVDPPIIHRDISIGNIVVGSSSSSSE
ETSAYLIDFATALAETSQSSDPETITGTYIFMACQVMLTGVHSIKTDLES
LFLVLLYLACDERVPWARAPSRRMSCALKIQALTMSHDTLFAQTRDDLLP
AVTSLHELFKILPAGSQWRDSCSISAAARAVHG*
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mRNA from alignment at Contig_101:494404..495105+

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_10827 ID=mRNA_10827|Name=jgi.p|Trimin1|173293|organism=Tribonema minus UTEX_B_3156 |type=mRNA|length=702bp|location=Sequence derived from alignment at Contig_101:494404..495105+ (Tribonema minus UTEX_B_3156 )
CATCCCGCGTCACGCACTCACGCCCATGTTGATGTGCAGGTCCACATGTT GCAACGCTTGGCGAGCATCAAGAATGTCATTCGCCTGGAGGGGCAGGGCG TCACCTGTGACGGGCACTTTTTGGTGACTTCGCCATACGGCCGCCGTCTG AAGCCCACAGACTCTGCGCACGTGATGCTGAGAGCAGTCCGTGACGTAGC ATCAGCGCTCGCCATGATGGCAGCTGTTGACCCGCCAATCATCCACAGGG ACATATCAATTGGCAACATTGTCGTTGGCAGCAGCAGCAGCAGCAGCGAG GAGACGAGCGCGTACCTGATAGACTTTGCCACTGCGCTCGCCGAGACAAG TCAGAGCTCTGACCCAGAGACCATCACTGGCACATACATCTTCATGGCTT GTCAGGTCATGCTGACTGGGGTGCACAGTATCAAGACAGACCTCGAGAGT CTATTCTTGGTGCTGCTATACCTGGCCTGTGATGAGCGGGTGCCCTGGGC ACGCGCACCCAGTCGCAGGATGTCGTGTGCGCTGAAGATCCAGGCACTCA CAATGTCGCATGACACTCTGTTTGCACAGACCCGTGATGATCTTCTCCCC GCAGTCACTAGTCTCCACGAGCTGTTTAAGATCTTACCAGCAGGCAGCCA ATGGCGTGACTCCTGCAGCATTTCTGCAGCGGCTCGAGCAGTGCATGGCT GA
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Coding sequence (CDS) from alignment at Contig_101:494404..495105+

>mRNA_10827 ID=mRNA_10827|Name=jgi.p|Trimin1|173293|organism=Tribonema minus UTEX_B_3156 |type=CDS|length=702bp|location=Sequence derived from alignment at Contig_101:494404..495105+ (Tribonema minus UTEX_B_3156 )
CATCCCGCGTCACGCACTCACGCCCATGTTGATGTGCAGGTCCACATGTT
GCAACGCTTGGCGAGCATCAAGAATGTCATTCGCCTGGAGGGGCAGGGCG
TCACCTGTGACGGGCACTTTTTGGTGACTTCGCCATACGGCCGCCGTCTG
AAGCCCACAGACTCTGCGCACGTGATGCTGAGAGCAGTCCGTGACGTAGC
ATCAGCGCTCGCCATGATGGCAGCTGTTGACCCGCCAATCATCCACAGGG
ACATATCAATTGGCAACATTGTCGTTGGCAGCAGCAGCAGCAGCAGCGAG
GAGACGAGCGCGTACCTGATAGACTTTGCCACTGCGCTCGCCGAGACAAG
TCAGAGCTCTGACCCAGAGACCATCACTGGCACATACATCTTCATGGCTT
GTCAGGTCATGCTGACTGGGGTGCACAGTATCAAGACAGACCTCGAGAGT
CTATTCTTGGTGCTGCTATACCTGGCCTGTGATGAGCGGGTGCCCTGGGC
ACGCGCACCCAGTCGCAGGATGTCGTGTGCGCTGAAGATCCAGGCACTCA
CAATGTCGCATGACACTCTGTTTGCACAGACCCGTGATGATCTTCTCCCC
GCAGTCACTAGTCTCCACGAGCTGTTTAAGATCTTACCAGCAGGCAGCCA
ATGGCGTGACTCCTGCAGCATTTCTGCAGCGGCTCGAGCAGTGCATGGCT
GA
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