mRNA_10523 (mRNA) Tribonema minus UTEX_B_3156

BLAST of jgi.p|Trimin1|170590 vs. uniprot
Match: A0A835YM61_9STRA (DNA topoisomerase (Fragment) n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YM61_9STRA)

HSP 1 Score: 1338 bits (3464), Expect = 0.000e+0
Identity = 753/753 (100.00%), Postives = 753/753 (100.00%), Query Frame = 1

Query:    1 RFDGLDQSIISYGPCQFPTLGFIVERYRRIQAFQREAFWSIAMEYCADAAGGARLPAPXXXXXXXXXXXXXXXXQATRASFMWERGRLFDRGVAAALAELCADAGTAVVTAVSARPQTRRRPLPMSTVELQRRASRFLRIPSERAMAAAEALYQRGVLSYPRTETDAFSRETDLAGLVGEFRGNAALGAYAAALLDGGAFEWPREGTNNDNAHPPIHPTASVDPATLHGDERGVYELVARHFLAACSRDARGQQTSVRAHMGEEFSASGLMITERNWLEIYHPWERWSDKAIPTFTVGQEFVPTSLMLTEGRTEPPPLLSESDLIQLMDANRIGTDATIAEHIKKIQERGYAERCHESRFRPTVLGLALVDGYEALGLKLSQPLLRQAQEQDCKLVARRELARADAIARCLSVMKPAFEKCVSDAHVLDAAIGAHFGALGARAACVTAAFAACGNCGTRMDLRVTDAGXXXXXXXXARPLLYCTTCRRGLPLPHRGEIALATPGVACPLCAYEVLEVRPGNGFEGNAYTICPWCYNNPPEEHGGAGVGTFPCFLCSADCPLAKKTGSDATPIAPCPLGCGNSLSIRSAXXXXXXXXXXXXXXXXXXXXXXSLSCSGYPACRGAVWLPRVVASARPAESGATCARCGPNVRRLTLTLRRAGRPAYVPPLLEERRGGGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPAPKITVRKDGPNQGRNFFKCATGECAFWEWEHDNWPEGGGG 2259
            RFDGLDQSIISYGPCQFPTLGFIVERYRRIQAFQREAFWSIAMEYCADAAGGARLPAPXXXXXXXXXXXXXXXXQATRASFMWERGRLFDRGVAAALAELCADAGTAVVTAVSARPQTRRRPLPMSTVELQRRASRFLRIPSERAMAAAEALYQRGVLSYPRTETDAFSRETDLAGLVGEFRGNAALGAYAAALLDGGAFEWPREGTNNDNAHPPIHPTASVDPATLHGDERGVYELVARHFLAACSRDARGQQTSVRAHMGEEFSASGLMITERNWLEIYHPWERWSDKAIPTFTVGQEFVPTSLMLTEGRTEPPPLLSESDLIQLMDANRIGTDATIAEHIKKIQERGYAERCHESRFRPTVLGLALVDGYEALGLKLSQPLLRQAQEQDCKLVARRELARADAIARCLSVMKPAFEKCVSDAHVLDAAIGAHFGALGARAACVTAAFAACGNCGTRMDLRVTDAGXXXXXXXXARPLLYCTTCRRGLPLPHRGEIALATPGVACPLCAYEVLEVRPGNGFEGNAYTICPWCYNNPPEEHGGAGVGTFPCFLCSADCPLAKKTGSDATPIAPCPLGCGNSLSIRSAXXXXXXXXXXXXXXXXXXXXXXSLSCSGYPACRGAVWLPRVVASARPAESGATCARCGPNVRRLTLTLRRAGRPAYVPPLLEERRGGGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPAPKITVRKDGPNQGRNFFKCATGECAFWEWEHDNWPEGGGG
Sbjct:    1 RFDGLDQSIISYGPCQFPTLGFIVERYRRIQAFQREAFWSIAMEYCADAAGGARLPAPXXXXXXXXXXXXXXXXQATRASFMWERGRLFDRGVAAALAELCADAGTAVVTAVSARPQTRRRPLPMSTVELQRRASRFLRIPSERAMAAAEALYQRGVLSYPRTETDAFSRETDLAGLVGEFRGNAALGAYAAALLDGGAFEWPREGTNNDNAHPPIHPTASVDPATLHGDERGVYELVARHFLAACSRDARGQQTSVRAHMGEEFSASGLMITERNWLEIYHPWERWSDKAIPTFTVGQEFVPTSLMLTEGRTEPPPLLSESDLIQLMDANRIGTDATIAEHIKKIQERGYAERCHESRFRPTVLGLALVDGYEALGLKLSQPLLRQAQEQDCKLVARRELARADAIARCLSVMKPAFEKCVSDAHVLDAAIGAHFGALGARAACVTAAFAACGNCGTRMDLRVTDAGXXXXXXXXARPLLYCTTCRRGLPLPHRGEIALATPGVACPLCAYEVLEVRPGNGFEGNAYTICPWCYNNPPEEHGGAGVGTFPCFLCSADCPLAKKTGSDATPIAPCPLGCGNSLSIRSAXXXXXXXXXXXXXXXXXXXXXXSLSCSGYPACRGAVWLPRVVASARPAESGATCARCGPNVRRLTLTLRRAGRPAYVPPLLEERRGGGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPAPKITVRKDGPNQGRNFFKCATGECAFWEWEHDNWPEGGGG 753          

BLAST of jgi.p|Trimin1|170590 vs. uniprot
Match: D7FUP7_ECTSI (DNA topoisomerase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FUP7_ECTSI)

HSP 1 Score: 623 bits (1607), Expect = 3.430e-211
Identity = 360/778 (46.27%), Postives = 446/778 (57.33%), Query Frame = 1

Query:    1 RFDGLDQSIISYGPCQFPTLGFIVERYRRIQAFQREAFWSIAMEYCADAAGGARLPAPXXXXXXXXXXXXXXXXQATRASFMWERGRLFDRGVAAALAELCADAGTAVVTAVSARPQTRRRPLPMSTVELQRRASRFLRIPSERAMAAAEALYQRGVLSYPRTETDAFSRETDLAGLVGEFRGNAALGAYAAALLDGGAFEWPREGTNNDNAHPPIHPTASVDPATLHGDERGVYELVARHFLAACSRDARGQQTSVRAHMG-EEFSASGLMITERNWLEIYHPWERWSDKAIPTFTVGQEFVPTSLMLTEGRTEPPPLLSESDLIQLMDANRIGTDATIAEHIKKIQERGYAERCHESRFRPTVLGLALVDGYEALGLKLSQPLLRQAQEQDCKLVARRELARADAIARCLSVMKPAFEKCVSDAHVLDAAIGAHFGALGA-----RAACVTAAFAACGNCGTRMDLRVTDAGXXXXXXXXARPLLYCTTCRRGLPLPHRGEIALATPGVACPLCAYE-------VLEVRPGNGFEGNAYTICPWCYNNPPEEHGGAGVGTFPCFLCSADCPLAKKT-GSDATPIAPCPLGCGNSLSIRSAXXXXXXXXXXXXXXXXXXXXXXSLSCSGYPAC-RGAVWLPRVVASARPAESGATCARC-GPN----VRRLTLTLRRAGRPAYVPPLLEERRGGGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPAPKITVRKDGPNQGRNFFKCATGE----CAFWEW-EHDNWPEGGGG 2259
            RFD L + +ISYGPCQFPTLGFIV+RY+RIQAFQ EAFWSI MEY  D     R P                     RA F W+RGRL+D    A L E+  + G AVVT+V AR  T+ RP+PMSTVELQ+RASRF  I SER M  AEALYQRG++SYPRTET+ F RE D+  L+G+FR N   G Y   LLD G F WPREG  +D+AHPPIHPT SVDP +L  DE+ +YELV +HFLA CS+DA G QT V A+ G E+F+A+GLM+TERNWL++Y P+ERW+ + IP F  GQEFVP+ L++ EGRT+ PPLLSESDLI LMD   IGTDATIAEHI+KIQ+R YA +  + RF PT LGLALVDGY+ +G ++S P LR A E DC  ++R E  +   + RC+S MK  F+ C + AH LD A+  HF  LG+     R       F+ CG CGT MDL+ + +G         RPLL+      G PLPH+G++    P   CPLC ++       VL V  GN + G+ YT+CP+C+NN P  HGG G   F CF+CSA CPLAK+  G D T +     GCG +L+I+                         L C GYPAC    VWLP+   +    E   TCARC GP     VRRL  T +   RP +     + R GGG                                   P         GPNQGR F+ C   +    C  + W + DN   G GG
Sbjct:    4 RFDELQEGVISYGPCQFPTLGFIVDRYKRIQAFQSEAFWSIKMEYAED---DPRSPGGK-----------------ARADFSWQRGRLYDHACCAILYEMTVEDGVAVVTSVEARRVTKARPIPMSTVELQKRASRFCHISSERTMHVAEALYQRGIISYPRTETEKFKREFDINTLLGDFRDNPRFGNYVGGLLDNGGFLWPREGRGDDSAHPPIHPTKSVDPNSLQADEKKIYELVTKHFLACCSKDAVGHQTKVLANCGGEDFTATGLMVTERNWLDVYEPFERWTGRNIPEFFQGQEFVPSVLVMDEGRTQAPPLLSESDLIGLMDQKGIGTDATIAEHIQKIQQRKYAVKNEQDRFYPTTLGLALVDGYDNIGFQMSAPTLRAAMELDCTRISRGEDTKEAVVERCISKMKECFQTCQALAHKLDDAVAMHFRPLGSSGGESRTVRGGRGFSRCGVCGTAMDLKKSSSG---------RPLLFXXXXXTGFPLPHKGKLTKQDPEFECPLCHFQARFLHVLVLSVSTGNDYTGSGYTVCPYCFNNAPAAHGGEGQSQFRCFMCSASCPLAKRVQGGDTTIVRCWKAGCGQALNIKRTRTGKYH-----------------LGCKGYPACSESTVWLPKNATNVSVTEE--TCARCSGPTAADAVRRLDFTFK-FQRPWFSARTAKGRGGGGVPSRPERNEPTGTSPLNTSQSPRFPSLRNIDRFGRPLL-------GPNQGRQFYTCPRSDRNEQCTTFTWLDGDNRRSGRGG 725          

BLAST of jgi.p|Trimin1|170590 vs. uniprot
Match: A0A1V9Z583_9STRA (DNA topoisomerase n=1 Tax=Achlya hypogyna TaxID=1202772 RepID=A0A1V9Z583_9STRA)

HSP 1 Score: 449 bits (1156), Expect = 5.170e-140
Identity = 283/672 (42.11%), Postives = 374/672 (55.65%), Query Frame = 1

Query:   16 DQSIISYGPCQFPTLGFIVERYRRIQAFQREAFWSIAMEYCADAAGGARLPAPXXXXXXXXXXXXXXXXQATRASFMWERGRLFDRGVAAALAELCADAGTAVVTAVSARPQTRRRPLPMSTVELQRRASRFLRIPSERAMAAAEALYQRGVLSYPRTETDAFSRETDLAGLVGEFRGNAALGAYAAALLDGGAFEWPREGTNNDNAHPPIHPTASVDPATLHGD-ERGVYELVARHFLAACSRDARGQQTSVRAHMGEE-FSASGLMITERNWLEIYHPWERWSDKAIPTFTVGQEFVPTSLMLTEGRTEPPPLLSESDLIQLMDANRIGTDATIAEHIKKIQERGYAERCH-ESRFRPTVLGLALVDGYEALGLKLSQPLLRQAQEQDCKLVARRELARADAIARCLSVMKPAFEKCVSDAHVLDAAIGAHFG-----ALGARAACVTAAFAACGNCGTRMDLRV-TDAGXXXXXXXXARPLLYCTTCRRGLPLPHRGEIALATPGVACPLCAYEVLEVRPGNGFEGNAYTICPWCYNNPPEEHGGAGVGTFPCFLCSADCPLAKKTGSDATPIAPCPLGCGNSLSIRSAXXXXXXXXXXXXXXXXXXXXXXSLSCSGYPACRGAVWLPRVVASARPAESGATCARCG-PN--VRRLTLTLRRAGRPAYV 1995
            +  ++S+G CQFPTLGF+V+R+  IQAF RE F+ I + +     G                             F W R R+FD     AL E C DA  A VT V+ +  ++R+PLP++TVE Q+RASR+LRI SE+AM AAEALY RG+LSYPRTETD+F   TDL  L+     +   G Y   LL GG FE PR G+++D AHPPIHPT SV  A +  D ER VYE V RHFLA CSRDARG QTSV   + +E F A GLMI ERN+L+IY  +E+W+   IP +  G  FVPT+L+L  G T PPPLL+ESDLI  MD + IGTDATIAEHI  I +R YA + + +++F+PT LGLALV  Y+ +G +L++P LR A E+DC  +A+ +      +  C+S MK  FE  V+ ++VLD     +FG     AL   +  V  AF+ACG C   M+LR  +D G           LL C  C     LP     A+A     CPLC ++V+E++   G  G  Y++CP C+N PP    G  +    C+ C+ADCPLA  T      +  C    G S+ ++                         LSC+ YPAC+ ++WLP  VA A    +   CARC  PN  V R+ L  + +  P  V
Sbjct:  207 ESRVVSFGSCQFPTLGFVVDRFLAIQAFVREPFYHIHVAHTIPDVGSI--------------------------VFSWNRARVFDFVAGLALFETCVDAQVATVTDVTKKETSKRKPLPLTTVEFQKRASRWLRISSEQAMTAAEALYNRGLLSYPRTETDSFKEGTDLPALLALHTDHEMWGGYVN-LLQGGKFEAPRRGSHDDQAHPPIHPTKSVALAAITNDVERRVYEFVVRHFLACCSRDARGSQTSVAMRLADETFGAKGLMILERNYLDIYK-YEKWAATTIPVYERGDTFVPTTLLLKAGETAPPPLLTESDLIAKMDQHGIGTDATIAEHINTILKRQYAVKVNNDTQFQPTELGLALVQTYDRMGYQLAKPDLRAAMERDCNAIAQGQKTCEQVVRDCMSQMKTIFESVVARSNVLDETFQGYFGEATPEALAVNSRVVDRAFSACGKCQQPMELRKKSDDGG----------LLVCRRCNELYALPLHS--AVAQSATRCPLCHFQVVEIQKAAGSAG--YSVCPHCFNAPP----GGRLNEMACYQCTADCPLATGTHEPFV-VEACIQCAGASMKLKKTKTAKYV-----------------LSCTEYPACKASLWLPAAVAHAMVTTT--PCARCSRPNQLVHRVGLRFQTSALPPDV 812          

BLAST of jgi.p|Trimin1|170590 vs. uniprot
Match: B7G3B1_PHATC (DNA topoisomerase n=2 Tax=Phaeodactylum tricornutum TaxID=2850 RepID=B7G3B1_PHATC)

HSP 1 Score: 446 bits (1148), Expect = 1.280e-139
Identity = 274/696 (39.37%), Postives = 377/696 (54.17%), Query Frame = 1

Query:    1 RFDGL-DQSIISYGPCQFPTLGFIVERYRRIQAFQREAFWSIAMEYCADAAGGARLPAPXXXXXXXXXXXXXXXXQATRASFMWERGRLFDRGVAAALAELCADAGTAVVTAVSARPQTRRRPLPMSTVELQRRASRFLRIPSERAMAAAEALYQRGVLSYPRTETDAFSRETDLAGLVGEFRG-NAALGAYAAALLDGGAFEWPREGTNNDNAHPPIHPTASVDPATLHGD-ERGVYELVARHFLAACSRDARGQQTSVRAHMG-EEFSASGLMITERNWLEIYHPWERWSDKA--IPTFTVGQEFVPTSLMLTEGRTEPPPLLSESDLIQLMDANRIGTDATIAEHIKKIQERGYAERCHESRFRPTVLGLALVDGYEALGLKLSQPLLRQAQEQDCKLVARRELARADAIARCLSVMKPAFEKCVSDAHVLDAAIGAHFGALGA---RAACVTAAFAACGNCGTRMDLRVT--DAGXXXXXXXXARPLLYCTTCRRGLPLPHRGEIALAT------PGVACPLCAYEVLEVRPGNGFEGNAYTICPWCYNNPPEEHGGAG-VGTFPCFLCS-ADCPLAKKTGSDATPIAPCPLGCGNSLSIRSAXXXXXXXXXXXXXXXXXXXXXXSLSCSGY----PACRGAVWLPR----VVASARPAESGATCARCGPNVRRLTLTLRRAGRPAYVPPLL 2007
            +FDG  +Q +ISYGPCQFPTLGF+VER+ RI+ F  E FW + +    D     +                    Q     F W+RG L+D+ +   L E C +AG AVVTA++ R + + RP+P++TVELQ+RAS +LRI SE  M+AAE LYQ+G +SYPRTET+ F  E +   L+ +F       GAYA+ LL+   F+ PR G ++D AHPPI P  +VDP T+    +R +Y L+ +H+LA CSRDA G+ T++   MG EEF+A+GLM+ E+NWLEIY PWERW      +P   VG    PTS ++ EGR+ PP  +SE +LI LMD N IGTDATIA+HI  I +R YA +    +F PT LG+ALV+GY ++G +L++P LR+  E +C  VA     + + +   L+ MK  +E   ++A  LD A+  HF  LGA    +  V  +F+ CG C   M L+    +           R LLYC+TCR G  LP RG +   T      P V CP+C ++V+ +  G G+EGN Y +CP C+++PP +HGGA   G F CF C    C LA  T      +  CP  C  S    S                        LSC+ Y      C   +WLP+    V   +        C RC  + R +   +    +P  VPP L
Sbjct:  256 KFDGFAEQGVISYGPCQFPTLGFVVERWARIETFIPEDFWHLELAISVDDTYHQQ------SQEEDQSAARGRTQQNRTIHFSWKRGYLYDKLLTTVLFEECLEAGEAVVTAMNGRTRNKWRPVPLATVELQKRASMYLRIGSETLMSAAEELYQQGYISYPRTETERFRPEFEHRPLIQQFSSLQGEFGAYASKLLNENGFQIPRAGKSDDQAHPPITPAKAVDPNTIQDQIQRKIYSLIVKHYLACCSRDAVGRGTTLTVRMGTEEFNATGLMVIEKNWLEIYSPWERWGSGQGELPPLQVGSRIRPTSFLMKEGRSGPPQPISEVELISLMDRNGIGTDATIAQHISTILDREYARKDGRQKFLPTPLGIALVEGYNSMGYQLNKPDLRREMEAECNEVASGRKTKEEIMVPILAKMKSCYETARAEARKLDEAVARHFPRLGAGESTSQVVEESFSECGVCRNSMALKQERENNNRTTARNTVRRKLLYCSTCRAGWTLP-RGVVRPKTEQEDNGPPVKCPICQFQVIRILRGEGYEGNGYHVCPKCFSDPPSDHGGASNAGDFRCFACQHPTCALASGTPGGDVEVFRCPF-CHPSAQPTSTSDSGKVCVRKTSRGYV-------LSCNKYVRGQDRCSYTIWLPKECHKVSVLSGDENQNEICGRCS-SPRAVIRKVHFVWKPGSVPPHL 935          

BLAST of jgi.p|Trimin1|170590 vs. uniprot
Match: A0A4D9CZ97_9STRA (DNA topoisomerase n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9CZ97_9STRA)

HSP 1 Score: 448 bits (1152), Expect = 1.090e-138
Identity = 281/683 (41.14%), Postives = 362/683 (53.00%), Query Frame = 1

Query:    1 RFDGLDQSIISYGPCQFPTLGFIVERYRRIQAFQREAFWSIAMEYCADAAGGARLPAPXXXXXXXXXXXXXXXXQATRASFMWERGRLFDRGVAAALAELCADAGTAVVTAVSARPQTRRRPLPMSTVELQRRASRFLRIPSERAMAAAEALYQRGVLSYPRTETDAFSRETDLAGLVGEFRGNAALGAYAAALLDGGA--------------FEWPREGTNNDNAHPPIHPTASVDPATLHG-DERGVYELVARHFLAACSRDARGQQTSVRAHMG-EEFSASGLMITERNWLEIYHPWERWSDKAIPT--FTVGQEFVPTSLMLTEGRTEPPPLLSESDLIQLMDANRIGTDATIAEHIKKIQERGYAERCH------------ESRFRPTVLGLALVDGYEALGLKLSQPLLRQAQEQDCKLVARRELARADAIARCLSVMKPAFEKCVSDAHVLDAAIGAH---FGALGARAAC--------VTAAFAACGNCGTRMDLRVTDAGXXXXXXXXARPLLYCTTCRRGLPLPHR----GEIALATPGVACPLCAYEVLEVRP-GNGFEGNA--------YTICPWCYNNPPEEHGGAGVGTFPCFLCSA--DCPLAKKTGSDATPI-APCPLGCGNSLSIRSAXXXXXXXXXXXXXXXXXXXXXXSLSCSGYPACRGAVWL 1878
            +FDG++ SIISYGPCQFPT+GF+V+RY+ I++FQRE FW I M Y      G   P                    TR  F W+R RLFD    A L  +  +A  A VT V+A P TR RPLP+STVELQ++ASRF  + S+  M  AE LY +G +SYPRTET++FSR+ DL  L+ E   + A GA+A  L+ GG               F +PR+G+ +D AHPPIHPT    P  L G DE+ VYELV RHFLA CS DA+G++T V   MG EEF+A+G++I ERNWL++Y+ WE+W    +P     VG  FVPTSL L  GRT+PPPLLSE+DLI  MD   IGTDATIA+HIK I +R Y                 + RF+PT LGL LV+GYE +G +L++P LR   E++CK VAR E  R + +  CL  MK  FE C +    L  A+G +   +G  G R           + A F+ CG+C  RMDLR   +             L C TC     LPH        +  TP V CPLC +E L + P G G EG          Y +CPWCY++PP    G  +  F C  C+   D  L  +       +  PCPL     L    A                       L C G+PAC+G ++ 
Sbjct:  202 KFDGVEDSIISYGPCQFPTMGFVVDRYKAIESFQREEFWRIQMAYRGARENGDEGPQGF-----------------TR--FQWKRERLFDEAACAVLYVMVVEAKEARVTYVNAAPATRDRPLPLSTVELQKKASRFFNMSSDVCMHVAEELYTKGFISYPRTETESFSRDADLRSLIAEQGRHPAWGAFARELVGGGEEEGGQHGRDSGAGKFRFPRKGSKDDQAHPPIHPTKLAAPQELKGTDEQKVYELVVRHFLACCSDDAKGKRTKVTVEMGGEEFTATGIVILERNWLDVYNGWEKWHASTLPANVLQVGAAFVPTSLTLETGRTQPPPLLSEADLISRMDEKGIGTDATIAQHIKTILQRKYVTXXXXXXXXXXXXXXLQRRFQPTNLGLGLVEGYEEIGYQLTKPELRAEMERECKRVARGERGRTEMVQACLLKMKECFEDCQNQWERLPEAVGKYLRRYGQGGGREXXXXXGTIRTMQAGFSQCGHCQGRMDLRRDKSVL----------FLACETCAASHLLPHSRKGTSRPSTLTPPVLCPLCGFEALLITPEGGGREGGQGDERVPPPYHMCPWCYHHPPAS--GTAMDNFRCANCTGVLDRGLEGRENDGGGGVRGPCPLARA-VLDADKAVLSCRGCKGRHSMTLRKNERGLMLGCQGFPACKGTIYF 852          

BLAST of jgi.p|Trimin1|170590 vs. uniprot
Match: A0A7S2LVS8_9STRA (DNA topoisomerase n=1 Tax=Skeletonema marinoi TaxID=267567 RepID=A0A7S2LVS8_9STRA)

HSP 1 Score: 437 bits (1125), Expect = 4.140e-138
Identity = 279/692 (40.32%), Postives = 374/692 (54.05%), Query Frame = 1

Query:    1 RFDGL-DQSIISYGPCQFPTLGFIVERYRRIQAFQREAFWSIAMEYCADAAGGARLPAPXXXXXXXXXXXXXXXXQATRASFMWERGRLFDRGVAAALAELCADAGTAVVTAVSARPQTRRRPLPMSTVELQRRASRFLRIPSERAMAAAEALYQRGVLSYPRTETDAFSRETDLAGLVGEFRGNAA-LGAYAAALLDGGAFEWPREGTNNDNAHPPIHPTASVDPATLHGD-ERGVYELVARHFLAACSRDARGQQTSVRAHMG-EEFSASGLMITERNWLEIYHPWERW--SDKAIPTFTVGQEFVPTSLMLTEGRTEPPPLLSESDLIQLMDANRIGTDATIAEHIKKIQERGYAERCHESRFRPTVLGLALVDGYEALGLKLSQPLLRQAQEQDCKLVARRELARADAIARCLSVMKPAFEKCVSDAHVLDAAIGAHFGALGARAA---CVTAAFAACGNCGTRMDLRVTDAGXXXXXXXX---ARPLLYCTTCRRGLPLP-HRGEIALATPGV---------ACPLCAYEVLEVRPGNGFEGNAYTICPWCYNNPPEEHGGAGVGTFPCFLCS-ADCPLAKKTGSDATPIAPCPLGCGNSLSIRSAXXXXXXXXXXXXXXXXXXXXXXSLSCS--GYPACRGAVWLPRVVASARPAESGATCARCGPNVRRLTLTLRRAGRPAYVPP 2001
            +FDG  D  +ISYGPCQFPTLGF+VER+ RI+ F  E FW+I M          RL                   Q+      W+RGRL+DR +  AL + C +AG AVVT+++ RP+ + RP+P++TVELQ+RAS++LRI SE  M  AE LY +G +SYPRTET+ F  E +   L+  F+  A   G YA  LL    F+ PR G N+DNAHPPI P  +VDP T+    +R +Y LV +H+LA CSRDA G++T +   +  EEF A GLMI ERNWL+IY PWE+W      +P   +G   VPTSL +  G+T PP L+SE +LI LMD N IGTDATIA+HI  I +R YAE+    RF PT LG+ALV+GY ++G +L++P LR+  E +C  VA     R   +   LS M   F+   ++ H LD A+  HF  LG+ ++    + A F  CG+C     L+    G  XXXXXX      LL C  C +   +P +  E+    PGV          CP+C ++V+E       +G  Y +CP C+++PPEEHGG     FPC  C+   C LAK T      I  CP    +  S                           LSCS  G  +C+  VWLP+        +S  +C RC  N  R+   L+   +P  VPP
Sbjct:   38 KFDGFQDSGVISYGPCQFPTLGFVVERWARIETFVPEDFWTIEMSI--------RLNGDGSVNNSDQNGNSNNNQQSRPIKLTWKRGRLYDRAMTMALCDACQEAGEAVVTSLTGRPKNKWRPVPLATVELQKRASKYLRIGSEELMQKAEELYHQGFISYPRTETEKFRPEFNHHTLIQSFQAVAGEFGDYANRLLSDNNFQNPRAGQNDDNAHPPITPAKAVDPQTIADPTQRKIYTLVVKHYLACCSRDAVGRETELTLRIASEEFVAKGLMILERNWLDIYQPWEKWYTGQGELPRARIGSRIVPTSLSMNAGQTSPPQLISEVELIALMDRNGIGTDATIAQHITTIMDRKYAEKDGNQRFHPTKLGIALVEGYNSMGYQLNKPDLRREMESECNAVAAGRKTRDQILGPILSKMLECFKIVNAEVHKLDEAVARHFNNLGSDSSQYRVLQANFTRCGSCNGMTVLKQRTGGGSXXXXXXXXGGSKLLQCAPCSKSFLMPRYNREVR---PGVNPNNQHEPLICPICQFQVVECVKD---DGGTYFVCPQCFSDPPEEHGGDSTD-FPCTKCTHPTCSLAKGTRGGDVEIFSCPFCASSGTSAGKVCLKRGQHGYR-------------LSCSNGGPNSCQFTVWLPKEAREIVIDDSTGSCNRCS-NPNRVVRQLKFIWKPGSVPP 700          

BLAST of jgi.p|Trimin1|170590 vs. uniprot
Match: A0A1Z5KJN8_FISSO (DNA topoisomerase n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5KJN8_FISSO)

HSP 1 Score: 433 bits (1114), Expect = 2.300e-135
Identity = 268/689 (38.90%), Postives = 374/689 (54.28%), Query Frame = 1

Query:   19 QSIISYGPCQFPTLGFIVERYRRIQAFQREAFWSIAMEYCADAAGGARLPAPXXXXXXXXXXXXXXXXQATRASFMWERGRLFDRGVAAALAELCADAGTAVVTAVSARPQTRRRPLPMSTVELQRRASRFLRIPSERAMAAAEALYQRGVLSYPRTETDAFSRE-------TDLAGLVGEFRGNAALGAYAAALLDGGAFEWPREGTNNDNAHPPIHPTASVDPATL-HGDERGVYELVARHFLAACSRDARGQQTSVRAHMGEE-FSASGLMITERNWLEIYHPWERWS--DKAIPTFTVGQEFVPTSLMLTEGRTEPPPLLSESDLIQLMDANRIGTDATIAEHIKKIQERGYAERCHESRFRPTVLGLALVDGYEALGLKLSQPLLRQAQEQDCKLVARRELARADAIARCLSVMKPAFEKCVSDAHVLDAAIGAHFGALGARAACVTAAFAACGNCGTRMDLRVTDAGXXXXXXXXARPLLYCTTCRR------GLPLPHRGEIALATPGVACPLCAYEVLEVRPGNGFEGNAYTICPWCYNNPPEEHGGAGVGTFPCFLCS-ADCPLAKKTGSDATPIAPCPLGCGNSLSIRSAXXXXXXXXXXXXXXXXXXXXXXSLSCSGYPA---CRGAVWLPR-------VVASARPAESG--ATCARCGPNVRRLTLTLRRAGRPAYV 1995
            + ++SYGPCQFPTLGF+VER+ RIQ F  E FW + +   AD A                         A    F W+R RL+D+     L E C DA  A+V + + RP+ + RP+P++TVELQ+RASRFL+I SE+ M AAE LYQ+G +SYPRTET+ F  E         LA + GE      +G  A  LL  G F+ PR G N+D AHPPI P  +VDPA++ +  +R VYELV +H+LA CSRDA G++T +   MGEE F A+GLM+ ERNWL+ Y PWERWS     +P+  VG    PTSL + EGRT PP  LSE +LI LMD + IGTDATIA+HI  IQ+R YA +    +F PT LG+ALV+GY ++G +L++P LR+  E +C L+A  +    + + R L  M+  +   V +AH+LD A+  H+ +LG  +  V   F  CG C   M L+    G         R +L+C +C R      G+P P R         V CPLC Y+V+++  G+G+ G+ Y +CP C++NPP  H       F C  C+  +CPL+  T  D T +  CP         +S                        +SC+ +     C  ++WLP+       +  ++   E G  ATC+R G  V ++  T +R   P ++
Sbjct:  217 EGVVSYGPCQFPTLGFVVERWARIQTFAPEDFWFLELTLTADEAN------------------------ARPIIFQWKRTRLYDQLTTLILYEACLDAQDAIVISANGRPKHKWRPVPLATVELQKRASRFLQISSEQLMTAAEELYQQGYISYPRTETERFRPEFQHRPLLEQLATMPGE------MGELAQRLLLPGHFQNPRAGPNDDQAHPPITPCKAVDPASIGNHQQRRVYELVVKHYLACCSRDAVGRETQLVVRMGEEEFQATGLMVLERNWLDAYRPWERWSTGQGELPSLPVGSRIRPTSLTMKEGRTTPPSPLSEVELISLMDRHGIGTDATIAQHIATIQDREYAVKDGNLKFSPTQLGIALVEGYNSMGYQLNKPDLRRETEAECNLIAAGQKTAEEVVLRLLEKMQQCYRTAVQEAHLLDRAVARHYQSLGDTSTVVQEQFTLCGTCRHPMTLKHNIQGRV------TRKMLHCASCSRTWALPRGIPQPKRDA---QNQPVICPLCQYQVVQITTGDGYTGSGYNVCPHCFSNPPPGH----APDFRCLNCTNIECPLSLGT-PDVT-LFHCPFCASQGNPTQSVTLRKTSRGYV-------------VSCTHFQRGNRCPYSIWLPKECEEVTVLDVTSNENEDGLCATCSRPGHLVLKMKFTWKRGSVPPHL 847          

BLAST of jgi.p|Trimin1|170590 vs. uniprot
Match: A0A7S4EJM4_9STRA (DNA topoisomerase n=1 Tax=Pseudo-nitzschia australis TaxID=44445 RepID=A0A7S4EJM4_9STRA)

HSP 1 Score: 434 bits (1115), Expect = 1.770e-134
Identity = 286/720 (39.72%), Postives = 386/720 (53.61%), Query Frame = 1

Query:    7 DGLDQSIISYGPCQFPTLGFIVERYRRIQAFQREAFWSIAMEYCADAAGGARLP--APXXXXXXXXXXXXXXXX------QATRASFMWERGRLFDRGVAAALAELCADAG--TAVVTAVSARPQTRRRPLPMSTVELQRRASRFLRIPSERAMAAAEALYQRGVLSYPRTETDAFSRETDLAGLVGEFRG-NAALGAYAAA---LLDGGAFEWPREGTNNDNAHPPIHPTASVDPATLHGD-ERGVYELVARHFLAACSRDARGQQTSVRAHMG-EEFSASGLMITERNWLEIYHPWERWS--DKAIPTFTVGQEFVPTSLMLTEGRTEPPPLLSESDLIQLMDANRIGTDATIAEHIKKIQERGYAERCHESRFRPTVLGLALVDGYEALGLKLSQPLLRQAQEQDCKLVARRELARADAIARCLSVMKPAFEKCVSDAHVLDAAIGAHFGALGA---RAACVTAAFAACGNCGTRMDLR-----------VTDAGXXXXXXXXARPLLYCTTCRRGLPLPHRGEIALATPG--------VACPLCAYEVLEVRPGNGFEGNAYTICPWCYNNPPEEHGGAGVG----TFPCFLCS-ADCPLAKKTGSDATPIAPCPLGC------GNSLSIRSAXXXXXXXXXXXXXXXXXXXXXXSLSCSGYPA---CRGAVWLPRVVASAR-PAESGATCARCGPN--VRRLTLTLRRAGRPAYV 1995
            +G    ++SYGPCQFPTLGF+VER+ RI+ F  + FW + +         A  P  AP             XXX       A   +F W+R RL+DR    A+ E C D     A VT +  RP+ + RP+P++T+ELQ+RASR+LRI SE  M+AAE LYQ+G++SYPRTET+ F  E D   L+ +F   + A G YA+    LL+   F+ PR G N+D AHPPI P   VDP T+    +R VY LV++H+LA CSRDA G++TS+   +  EEF+A GLMI ++NWLEIYHPWERWS     +P   VG    P SL++ EG+T PP  +SE +LI LMD   IGTDATIA+HI  IQ+R Y  +    RF PT LG+ALV+GY ++G +L++P LR+  E +C LVA R+  + + +   L+ MK  + K   DA  LD A+  HF  +GA       V A F+ CGNC   M L+                XXXXXX   R LL+C  C+ G  +P RG++   T          V CP+C ++V+ +  G+G+EGN Y ICP CY++PP +HG           PCF CS   C LA  T      +  CP  C      G  +++R                         LSC+ Y A   C   +W+PR   S   P      C RC     VR+LT   +  G P ++
Sbjct:  226 NGGSSGVVSYGPCQFPTLGFVVERWARIEIFVPDDFWFLELTLKVPQQAVAPPPPAAPDTDGSNRTNNADRXXXXXXXXXNARPIAFTWKRQRLYDRLATLAIYESCLDTTPCVATVTKLEGRPKNKWRPVPLATIELQKRASRYLRIGSETLMSAAEELYQQGLISYPRTETERFRPEFDHHALLRDFCSLDEAAGPYASYANHLLNENGFQNPRAGQNDDQAHPPITPCKVVDPNTIQDHTQRNVYILVSKHYLACCSRDAVGRETSLTVRIASEEFTAKGLMIEQKNWLEIYHPWERWSTGQGELPHLMVGSRIAPHSLVMKEGQTTPPLPISEPELITLMDKAGIGTDATIAQHITTIQDRDYVTKDGNLRFLPTPLGIALVEGYNSMGYQLNKPDLRRETEHECNLVANRQKTKEEIVPLILNKMKDCYLKATRDARKLDQAMARHFQRIGAGNDHTEVVQAQFSTCGNCQQPMALKQERRXXXXXXXXXXXXXXXXXXDVPRKLLFCNACQEGYVMPRRGQLRPKTSEEGNNDNTPVKCPICNFQVISIVRGDGYEGNGYNICPKCYSDPPADHGXXXXXXXXTNHPCFSCSHPTCALAGGTQGGDIEVFACPF-CREKNITGGKVTLRKNTRGYV------------------LSCANYSARQRCSFTIWMPRASRSIEVPPGDEHVCTRCSTQSVVRKLTFQWKSGGVPPHM 926          

BLAST of jgi.p|Trimin1|170590 vs. uniprot
Match: A0A067D7B1_SAPPC (DNA topoisomerase n=3 Tax=Saprolegnia TaxID=4769 RepID=A0A067D7B1_SAPPC)

HSP 1 Score: 433 bits (1114), Expect = 7.610e-134
Identity = 266/660 (40.30%), Postives = 371/660 (56.21%), Query Frame = 1

Query:   16 DQSIISYGPCQFPTLGFIVERYRRIQAFQREAFWSIAMEYCADAAGGARLPAPXXXXXXXXXXXXXXXXQATRASFMWERGRLFDRGVAAALAELCADAGTAVVTAVSARPQTRRRPLPMSTVELQRRASRFLRIPSERAMAAAEALYQRGVLSYPRTETDAFSRETDLAGLVGEFRGNAALGAYAAALLDGGAFEWPREGTNNDNAHPPIHPTASVDPATLHGD-ERGVYELVARHFLAACSRDARGQQTSVRAHMGEE-FSASGLMITERNWLEIYHPWERWSDKAIPTFTVGQEFVPTSLMLTEGRTEPPPLLSESDLIQLMDANRIGTDATIAEHIKKIQERGYAERCHES-RFRPTVLGLALVDGYEALGLKLSQPLLRQAQEQDCKLVARRELARADAIARCLSVMKPAFEKCVSDAHVLDAAIGAHFGALGARAAC-----VTAAFAACGNCGTRMDLRVT-DAGXXXXXXXXARPLLYCTTCRRGLPLPHRGEIALATPGVACPLCAYEVLEVRPGNGFEGNAYTICPWCYNNPPEEHGGAGVGTFPCFLCSADCPLAKKTGSDATPIAPCPLGCG-NSLSIRSAXXXXXXXXXXXXXXXXXXXXXXSLSCSGYPACRGAVWLPRVVASARPAESGAT-CARCGPNVRRLTL 1962
            +  ++S+G CQFPTLGF+V+R+  I+AF RE F+ I + +     G                             F W RGR++D   A AL E C +A  A + ++S +  ++R+P P++TVE Q+RASR+LRI SE+AM AAEALY RG+LSYPRTETD+F   TDL  L+    G+   G Y AAL +G  FE PR G ++D AHPPIHPT  V  ++L+ D ER VYELV RHFLA CS DARG QT+V   +  E F+ASGLMI  RN+L+IY  +E+W+   IP +  G  FVPT+L+L  G T PPPLL+ESDLI  MD + IGTDATIAEHI  I +R YA + + + +F+PT LGLALV  Y+ +G +L++P LR A E+DC  +A+ + +    +  C+  M+  ++  V+ + VLD     +FG     A       V  AF+ACG C   M+LR   D G           LL C  C     LP    I  +T    CPLC ++V++++   G   N Y++CP C+N+PP     A +   PC+ C+ADC LA  TG+  +P+  C   CG + + ++                         +SC+ Y +C+ +VWLP  +A A       T C+R    + R+TL
Sbjct:  207 ESKVVSFGSCQFPTLGFVVDRHLAIEAFVRELFYHIHVTHAIPDVGSI--------------------------VFTWGRGRVYDHIAALALYESCVEAQIATINSISKKDTSKRKPFPLTTVEFQKRASRWLRISSEQAMTAAEALYNRGLLSYPRTETDSFKEGTDLRALLDLHAGHETWGGYVAALQNG-KFEAPRSGKHDDQAHPPIHPTKCVPLSSLNNDVERRVYELVVRHFLACCSMDARGSQTNVTMTLASETFTASGLMILARNYLDIY-VYEKWAATVIPVYERGDRFVPTTLLLKRGETAPPPLLTESDLIAKMDTHGIGTDATIAEHINTILKRQYAIKVNNNTQFKPTELGLALVQTYDRMGYQLAKPDLRAAVERDCNAIAQGQKSCDQVVRSCMDQMRTIYQNVVARSGVLDETFQGYFGVANQDAGAGNSRVVDGAFSACGKCHQPMELRKKPDDGS----------LLVCRRCDDQYALPMLANIQKST--TRCPLCQFQVVDIQRSAGT--NVYSVCPHCFNHPPN----ARLNEMPCYSCTADCALA--TGTHDSPVVQCCFQCGGHPMKLKKTRTGKYV-----------------VSCTDYQSCKESVWLPSSIAYASVTSHPCTNCSRPQQTIHRVTL 801          

BLAST of jgi.p|Trimin1|170590 vs. uniprot
Match: K0TAA4_THAOC (DNA topoisomerase (Fragment) n=1 Tax=Thalassiosira oceanica TaxID=159749 RepID=K0TAA4_THAOC)

HSP 1 Score: 425 bits (1093), Expect = 3.580e-132
Identity = 276/693 (39.83%), Postives = 365/693 (52.67%), Query Frame = 1

Query:   13 LDQSIISYGPCQFPTLGFIVERYRRIQAFQREAFWSIAMEYCADAAGGA--RLPAPXXXXXXXXXXXXXXXXQATRASFMWERGRLFDRGVAAALAELCADAGTAVVTAVSARPQTRRRPLPMSTVELQRRASRFLRIPSERAMAAAEALYQRGVLSYPRTETDAFSRETDLAGLVGEFRG-NAALGAYAAALLDGGAFEWPREGTNNDNAHPPIHPTASVDPATLHGD-ERGVYELVARHFLAACSRDARGQQTSVRAHMG-EEFSASGLMITERNWLEIYHPWERWS--DKAIPTFTVGQEFVPTSLMLTEGRTEPPPLLSESDLIQLMDANRIGTDATIAEHIKKIQERGYAERCHESRFRPTVLGLALVDGYEALGLKLSQPLLRQAQEQDCKLVARRELARADAIARCLSVMKPAFEKCVSDAHVLDAAIGAHFGALG---ARAACVTAAFAACGNCGTRMDLRV--TDAGXXXXXXXXARP--LLYCTTCRRGLPLPH-RGEIALATPG----VACPLCAYEVLEVRPGNGFEGNAYTICPWCYNNPPEEHGGAGVGTFPCFLCS-ADCPLAKKTGSDATPIAPCPLGCGNSLSIRSAXXXXXXXXXXXXXXXXXXXXXXSLSCS--GYPACRGAVWLPRVVASARPAESG--------ATCARCGPNVRRLTLTLRRAGRPAYVPP 2001
            LDQ +ISYGPCQFPTLGF+VER+ RIQ F +E FW + M    +A G     +PA                 Q    S  W+R RL+DR +  A+ + C +AG AVVT VS RP+ + RP+P++TVELQ+RAS++LRI SE  M  AE+LY  G +SYPRTET+ F  E +   L+  F   N  LG YA  LL    F+ PR G N+DNAHPPI P  +V+P  +    ER +Y LV +H+LA CSRDA G++T +   +  EEFSA GLMI ERNWLEIYHPWE+WS     +P   VG    PTSLM+ +G T PP L+SE +LI  MD N IGTDATIA+HI  I +RGY  +    RF P  LG+ALV+GY ++G +L++P LR++ E +C  +A     +   +   +  M   F +  + A  LD A+  HFG LG   ++ + +   F+ CGNC   M L+   + AG          P  +++C TC +   +P     I     G     +CP+C ++VL        +G     CP CYNNPP  HGG+    F C  C+   CPLAK        + PCP  C +S                             LSCS  G  AC+  VWLP        A+S         A C  C  N  +L   LR   +   VPP
Sbjct:  271 LDQ-VISYGPCQFPTLGFVVERWARIQTFVKEDFWFLEMTIRLNADGSVVNNIPAQGVNNVYNNQQ------QGRAISLTWKRVRLYDRTMTLAIYDACLEAGEAVVTDVSGRPKNKWRPIPLATVELQKRASKYLRIGSEVLMQKAESLYNNGYISYPRTETERFRPEFNHHDLIQSFHAVNGELGNYAQLLLSNNNFQNPRAGPNDDNAHPPITPAKAVNPEEIADPIERNIYSLVVKHYLACCSRDAVGRETELTLKIASEEFSAKGLMILERNWLEIYHPWEKWSTGQGVLPKARVGTRITPTSLMMRDGATAPPNLISEVELISAMDKNGIGTDATIAQHITTILDRGYCVKDASQRFNPNPLGIALVEGYNSMGHQLNKPDLRRSMEAECNAIADGRKTKEAVLGSVVLQMLQCFNEANAKAAKLDEAMERHFGRLGTGNSQYSVLQGNFSRCGNCNGMMTLKQVRSSAGNQNPRNNNQAPSKIVHCQTCSQAHMMPKFHSNITPYFDGNQQPTSCPICQFQVLTCE----NDGRNSQFCPNCYNNPPANHGGSPSLEFQCSSCTHRSCPLAKGVQGGDVEVYPCPF-CTSS------------GNAGGRVLLKRSDNGYRLSCSNGGRDACQYTVWLPGEAREITLADSSVNVNRMSQAVCQTCT-NQSQLVRKLRFVWKQGTVPP 938