mRNA_P-littoralis_Contig669.1.1 (mRNA) Pylaiella littoralis U1_48

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-littoralis_Contig669.1.1
Unique NamemRNA_P-littoralis_Contig669.1.1
TypemRNA
OrganismPylaiella littoralis U1_48 (Pylaiella littoralis U1_48 (Mung))
Homology
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BLAST of mRNA_P-littoralis_Contig669.1.1 vs. uniprot
Match: D7FVK4_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FVK4_ECTSI)

HSP 1 Score: 162 bits (409), Expect = 7.760e-45
Identity = 85/112 (75.89%), Postives = 97/112 (86.61%), Query Frame = 3 
Query:   69 MKVEATKFTEVGFHGQDVDRIIKDLVEVGITMTKKKQMEEHREEVKEKTESIIVDILVGEKSNPALRDSFENMLKNGMLEDMKKMKVPLNRSNTPDMPGVILSDNTRFSMPK 404
            +KVEATKFTEVGFHGQDVDRIIKDLVEVGITMTKKKQM EHRE VK  T+SII+D LVGEKS  + R+SFE++LK G LE+   +KVPLNR+NTPDMPGVI SDN+R +MPK
Sbjct:  156 IKVEATKFTEVGFHGQDVDRIIKDLVEVGITMTKKKQMAEHREAVKTTTDSIIIDCLVGEKSGASSRESFEDLLKKGALEEYN-IKVPLNRNNTPDMPGVIFSDNSRANMPK 266
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BLAST of mRNA_P-littoralis_Contig669.1.1 vs. uniprot
Match: A0A024GVM8_9STRA (Uncharacterized protein n=1 Tax=Albugo candida TaxID=65357 RepID=A0A024GVM8_9STRA)

HSP 1 Score: 88.6 bits (218), Expect = 7.240e-18
Identity = 48/94 (51.06%), Postives = 68/94 (72.34%), Query Frame = 3 
Query:   69 MKVEATKFTEVGFHGQDVDRIIKDLVEVGITMTKKKQMEEHREEVKEKTESIIVDILVGEKSNPALRDSFENMLKNGMLED-MKKMKVPLNRSN 347
            +KVEATKFTEVGFHG+DVD+II+DLVE  I + KK++ E  R++V++  E  I+D L+G  S    R +FEN+LK G +E+ M  + VP++R N
Sbjct:  195 IKVEATKFTEVGFHGRDVDQIIRDLVENAINLVKKQKQERVRQQVQQLVERRILDALIGPNSTANSRAAFENLLKTGEMEERMITIDVPVSRGN 288
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BLAST of mRNA_P-littoralis_Contig669.1.1 vs. uniprot
Match: F0Y6N6_AURAN (Uncharacterized protein n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0Y6N6_AURAN)

HSP 1 Score: 88.2 bits (217), Expect = 9.080e-18
Identity = 45/81 (55.56%), Postives = 60/81 (74.07%), Query Frame = 3 
Query:   69 MKVEATKFTEVGFHGQDVDRIIKDLVEVGITMTKKKQMEEHREEVKEKTESIIVDILVGEKSNPALRDSFENMLKNGMLED 311
            +KVEATKFTEVGFHG+DVD+II+DLVE  IT+T+KK+ E+ REE +   E  I+D L G  +  A + SF  ML+ G+L+D
Sbjct:  129 IKVEATKFTEVGFHGRDVDQIIRDLVEASITLTRKKKAEQFREEARRLVEGRILDTLTGLNAAQATQSSFREMLRKGVLDD 209
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BLAST of mRNA_P-littoralis_Contig669.1.1 vs. uniprot
Match: A0A8J2WET5_9STRA (Hypothetical protein n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2WET5_9STRA)

HSP 1 Score: 87.8 bits (216), Expect = 1.200e-17
Identity = 49/106 (46.23%), Postives = 69/106 (65.09%), Query Frame = 3 
Query:   69 MKVEATKFTEVGFHGQDVDRIIKDLVEVGITMTKKKQMEEHREEVKEKTESIIVDILVGEKSNPALRDSFENMLKNGMLEDMKKMKVPLNRSNTPDMPGVILSDNT 386
            +KVEATKFTEVGFHG+DVD+II+DLVE  I +T+K++ E  R E     E+ I+D L G  +  A + SF  ML+ G+L+D +++ V +  S  P   GVI  D +
Sbjct:  118 VKVEATKFTEVGFHGRDVDQIIRDLVEASIALTRKRKAEAFRAEAARAAETRILDTLTGLNAAQATQSSFREMLRKGVLDD-RQIDVDVPHSQPPQQSGVIQLDQS 222
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BLAST of mRNA_P-littoralis_Contig669.1.1 vs. uniprot
Match: B8BRY4_THAPS (Heat shock protein HslVU, ATPase n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B8BRY4_THAPS)

HSP 1 Score: 87.8 bits (216), Expect = 1.350e-17
Identity = 48/94 (51.06%), Postives = 71/94 (75.53%), Query Frame = 3 
Query:   69 MKVEATKFTEVGFHGQDVDRIIKDLVEVGITMTKKKQMEEHREEVKEKTESIIVDILVGEK---SNPALRDSFENMLKNGMLEDMK-KMKVPLN 338
            +KVEATKFTEVG+HG+DVD+I++DL++V + +TKK+Q E+ R E K   E  I+DIL+G     +N + RDSF +ML++G+LED + ++ VP N
Sbjct:  181 LKVEATKFTEVGYHGRDVDQIVRDLMDVSMALTKKRQTEKLRGEAKGLVEERILDILIGPDGTGTNKSSRDSFRSMLRDGLLEDHELEVDVPEN 274
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BLAST of mRNA_P-littoralis_Contig669.1.1 vs. uniprot
Match: A0A836CKY0_9STRA (ATP-dependent hsl protease ATP-binding subunit hslU n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CKY0_9STRA)

HSP 1 Score: 86.7 bits (213), Expect = 3.330e-17
Identity = 54/114 (47.37%), Postives = 72/114 (63.16%), Query Frame = 3 
Query:   69 MKVEATKFTEVGFHGQDVDRIIKDLVEVGITMTKKKQMEEHREEVKEKTESIIVDILV-------GEKSNPALRDSFENMLKNGMLEDMK-KMKVPLNRSNTPDMPGVILSDNT 386
            +KVEATKFTEVGFHG DVD+IIKDLVE+ ITM KK +ME HR   + K E  ++D+L+       G +S    R+SF  +L+ G+LED+   + VP +R      P + L  NT
Sbjct:  162 IKVEATKFTEVGFHGGDVDQIIKDLVEISITMVKKARMENHRAAARLKAEDTVLDLLLKAPIDLEGNRS----RESFRKLLRQGLLEDIAVDVDVPADRG----APSLQLDQNT 267
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BLAST of mRNA_P-littoralis_Contig669.1.1 vs. uniprot
Match: A0A7S4K9Y1_9STRA (Hypothetical protein n=2 Tax=Odontella aurita TaxID=265563 RepID=A0A7S4K9Y1_9STRA)

HSP 1 Score: 86.7 bits (213), Expect = 3.380e-17
Identity = 51/108 (47.22%), Postives = 71/108 (65.74%), Query Frame = 3 
Query:   69 MKVEATKFTEVGFHGQDVDRIIKDLVEVGITMTKKKQMEEHREEVKEKTESIIVDILVGEK----SNPALRDSFENMLKNGMLEDMK-KMKVPLNRSNTPDMPGVILS 377
            +KVEATKFTEVG+HG+DVD+II+DL+++ + +TKK+Q E+ REE K   E  I+D+L G      SN   RD+F  ML+NG L+D   ++ VP    N  D  G + S
Sbjct:  164 IKVEATKFTEVGYHGRDVDQIIRDLMDISMVLTKKRQTEKLREEAKALVEERIIDLLAGPSNTGPSNKRSRDTFRAMLRNGDLDDQTVEVDVP---ENAGDKGGALFS 268
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BLAST of mRNA_P-littoralis_Contig669.1.1 vs. uniprot
Match: A0A7S3JUS6_9STRA (Hypothetical protein n=1 Tax=Aureoumbra lagunensis TaxID=44058 RepID=A0A7S3JUS6_9STRA)

HSP 1 Score: 86.3 bits (212), Expect = 4.540e-17
Identity = 51/117 (43.59%), Postives = 76/117 (64.96%), Query Frame = 3 
Query:   69 MKVEATKFTEVGFHGQDVDRIIKDLVEVGITMTKKKQMEEHREEVKEKTESIIVDILVGEKSNPALRDSFENMLKNGMLEDMK-KMKVPLNRSNTPDM-------PGVILSDNTRFS 395
            +KVEATKFTEVGFHG+DVD+II+DLVEV + +T+KK+ EE R E K   E+ I+D L G  +    + SF +ML+ G+L+D +  + +P +RS    +       P  +L++  R+S
Sbjct:  159 IKVEATKFTEVGFHGRDVDQIIRDLVEVSMILTRKKKAEELRPEAKIAVENRILDTLTGSNAARTTQSSFRDMLRKGVLDDRQIDVDLPNDRSGGQGVIQVDTSNPNALLNEVLRYS 275
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BLAST of mRNA_P-littoralis_Contig669.1.1 vs. uniprot
Match: A0A5D6XXH3_9STRA (Uncharacterized protein n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6XXH3_9STRA)

HSP 1 Score: 84.7 bits (208), Expect = 1.560e-16
Identity = 54/113 (47.79%), Postives = 73/113 (64.60%), Query Frame = 3 
Query:   69 MKVEATKFTEVGFHGQDVDRIIKDLVEVGITMTKKKQMEEHREEVKEKTESIIVDILVGEKSNPALRDSFENMLKNGMLEDMK-KMKVPLNRS-NTPDMPGVILSDNTRFSMP 401
            +KVEATKFTEVGFHG+DVD+II+DLVE  ITM KK++ E  R+EV+   ES I+D L G       R SFE +L+ G +ED    + VP+ R+ N  +M G I    +  ++P
Sbjct:  145 VKVEATKFTEVGFHGKDVDQIIRDLVENAITMVKKQRKERVRKEVQHLVESRILDALTGANVTERSRASFERLLRLGEMEDRTITVDVPVARNHNANNMNGPISFVTSEKALP 257
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BLAST of mRNA_P-littoralis_Contig669.1.1 vs. uniprot
Match: A0A7S2RAH1_9STRA (Hypothetical protein n=1 Tax=Eucampia antarctica TaxID=49252 RepID=A0A7S2RAH1_9STRA)

HSP 1 Score: 84.7 bits (208), Expect = 1.580e-16
Identity = 47/110 (42.73%), Postives = 75/110 (68.18%), Query Frame = 3 
Query:   69 MKVEATKFTEVGFHGQDVDRIIKDLVEVGITMTKKKQMEEHREEVKEKTESIIVDILVGEKSNPAL-------RDSFENMLKNGMLEDMKKMKVPLNRSNTPDMPGVILS 377
            +KVEATKFTEVG+HG+DVD+I++DL+++ + +TKKK+ +E REE K   E  I+D+L+G  S           RDSF +ML+ G+L++ +++++ +  S+     GV LS
Sbjct:  145 IKVEATKFTEVGYHGRDVDQIVRDLMDISMNLTKKKKTDELREEAKLLVEDRILDLLIGSPSTTPSETQKGRGRDSFRDMLRQGLLDE-QEVEIEVPESSPDKSNGVFLS 253
The following BLAST results are available for this feature:
BLAST of mRNA_P-littoralis_Contig669.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FVK4_ECTSI7.760e-4575.89Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
A0A024GVM8_9STRA7.240e-1851.06Uncharacterized protein n=1 Tax=Albugo candida Tax... [more]
F0Y6N6_AURAN9.080e-1855.56Uncharacterized protein n=1 Tax=Aureococcus anopha... [more]
A0A8J2WET5_9STRA1.200e-1746.23Hypothetical protein n=1 Tax=Pelagomonas calceolat... [more]
B8BRY4_THAPS1.350e-1751.06Heat shock protein HslVU, ATPase n=1 Tax=Thalassio... [more]
A0A836CKY0_9STRA3.330e-1747.37ATP-dependent hsl protease ATP-binding subunit hsl... [more]
A0A7S4K9Y1_9STRA3.380e-1747.22Hypothetical protein n=2 Tax=Odontella aurita TaxI... [more]
A0A7S3JUS6_9STRA4.540e-1743.59Hypothetical protein n=1 Tax=Aureoumbra lagunensis... [more]
A0A5D6XXH3_9STRA1.560e-1647.79Uncharacterized protein n=1 Tax=Pythium brassicum ... [more]
A0A7S2RAH1_9STRA1.580e-1642.73Hypothetical protein n=1 Tax=Eucampia antarctica T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-littoralis_Contig669contigP-littoralis_Contig669:10547..12406 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Pylaiella littoralis U1_48 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start1
Seed ortholog2880.D7FVK4
Preferred nameMCX1
PFAMsAAA_2,ClpB_D2-small
Model size405
Max annot lvl2759|Eukaryota
KEGG koko:K03544,ko:K03667,ko:K19852
KEGG Pathwayko04112,map04112
Hectar predicted targeting categorysignal peptide
GOsGO:0000166,GO:0003674,GO:0003824,GO:0004176,GO:0005488,GO:0005524,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0005759,GO:0006457,GO:0006508,GO:0006605,GO:0006626,GO:0006807,GO:0006810,GO:0006839,GO:0006886,GO:0006996,GO:0007005,GO:0008104,GO:0008144,GO:0008150,GO:0008152,GO:0008233,GO:0009056,GO:0009057,GO:0009893,GO:0009987,GO:0010604,GO:0015031,GO:0015833,GO:0016043,GO:0016462,GO:0016787,GO:0016817,GO:0016818,GO:0016887,GO:0017038,GO:0017076,GO:0017111,GO:0019222,GO:0019538,GO:0030150,GO:0030163,GO:0030554,GO:0031323,GO:0031325,GO:0031399,GO:0031401,GO:0031974,GO:0032268,GO:0032270,GO:0032553,GO:0032555,GO:0032559,GO:0033036,GO:0033365,GO:0034613,GO:0035639,GO:0036094,GO:0042026,GO:0042623,GO:0042886,GO:0043167,GO:0043168,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0044238,GO:0044422,GO:0044424,GO:0044429,GO:0044444,GO:0044446,GO:0044464,GO:0044743,GO:0045184,GO:0046907,GO:0048518,GO:0048522,GO:0050789,GO:0050794,GO:0051171,GO:0051173,GO:0051179,GO:0051234,GO:0051246,GO:0051247,GO:0051641,GO:0051649,GO:0055085,GO:0060255,GO:0065002,GO:0065007,GO:0070011,GO:0070013,GO:0070585,GO:0070727,GO:0071702,GO:0071704,GO:0071705,GO:0071806,GO:0071840,GO:0072594,GO:0072655,GO:0080090,GO:0097159,GO:0097367,GO:0140096,GO:1901265,GO:1901363,GO:1901564,GO:1901565,GO:1901575,GO:1904285,GO:1904287,GO:1990542
Exons4
Evalue1.19e-46
EggNOG OGsCOG1219@1|root,KOG0745@2759|Eukaryota
EC2.7.11.1
Descriptionunfolded protein binding
Cds size375
COG categoryO
BRITEko00000,ko00001,ko01000,ko01001,ko03110,ko04131
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680791389.6062183-UTR-P-littoralis_Contig669:10546..105751680791389.6062183-UTR-P-littoralis_Contig669:10546..10575Pylaiella littoralis U1_48UTRP-littoralis_Contig669 10547..10575 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680791389.6202264-CDS-P-littoralis_Contig669:10575..106951680791389.6202264-CDS-P-littoralis_Contig669:10575..10695Pylaiella littoralis U1_48CDSP-littoralis_Contig669 10576..10695 +
1680791389.6347008-CDS-P-littoralis_Contig669:11089..111511680791389.6347008-CDS-P-littoralis_Contig669:11089..11151Pylaiella littoralis U1_48CDSP-littoralis_Contig669 11090..11151 +
1680791389.6467254-CDS-P-littoralis_Contig669:11669..117841680791389.6467254-CDS-P-littoralis_Contig669:11669..11784Pylaiella littoralis U1_48CDSP-littoralis_Contig669 11670..11784 +
1680791389.6621573-CDS-P-littoralis_Contig669:12328..124061680791389.6621573-CDS-P-littoralis_Contig669:12328..12406Pylaiella littoralis U1_48CDSP-littoralis_Contig669 12329..12406 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-littoralis_Contig669.1.1prot_P-littoralis_Contig669.1.1Pylaiella littoralis U1_48polypeptideP-littoralis_Contig669 10576..12406 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-littoralis_Contig669.1.1

>prot_P-littoralis_Contig669.1.1 ID=prot_P-littoralis_Contig669.1.1|Name=mRNA_P-littoralis_Contig669.1.1|organism=Pylaiella littoralis U1_48|type=polypeptide|length=125bp
MVLLLLLLLLMLFMKVEATKFTEVGFHGQDVDRIIKDLVEVGITMTKKKQ
MEEHREEVKEKTESIIVDILVGEKSNPALRDSFENMLKNGMLEDMKKMKV
PLNRSNTPDMPGVILSDNTRFSMPK
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mRNA from alignment at P-littoralis_Contig669:10547..12406+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-littoralis_Contig669.1.1 ID=mRNA_P-littoralis_Contig669.1.1|Name=mRNA_P-littoralis_Contig669.1.1|organism=Pylaiella littoralis U1_48|type=mRNA|length=1860bp|location=Sequence derived from alignment at P-littoralis_Contig669:10547..12406+ (Pylaiella littoralis U1_48)
CTGTTCTGTTGCCGCCGCTGTTCCCACTAATGGTGTTGCTGTTGTTGTTG
TTGTTGTTGATGTTGTTCATGAAGGTGGAGGCGACAAAGTTCACTGAGGT
CGGGTTTCACGGCCAGGACGTGGACCGGATCATCAAGGATCTTGTAGAGG
TGAGGATCCAGAACACTGCAGCATACATTACCGTAACGTCGTACGATACC
TCCTTGGTAGATTTGGCAGCTCTGGCAGTAATTCCTGTCCGGACACATTT
ATTTGCCAGATCCCGGCAATTCTCCCAGCCCCGTTCCCGTTGATAGTGTT
TTATGATCTTTTTGACAATGGTTTCGACGTGATGCGATGGAGAAGGGGGT
GGATGGCGTGCTGTGCTTATTTTGTGATGCGATATGGTGTGATGTGACAC
GATGCACGTGTTCTGGCGTGATTTTTGTGTGAAAGCCCCAAGGCTACAGG
CAACGCGTTTTGGTGTGAATGATGTCTGATGCGATCGGAAACGATGCGAC
CTGGTTAACATGCAATGGATGATGACACCGGCGTGACTGGCAGGTGGGCA
TCACCATGACGAAAAAGAAGCAGATGGAGGAGCACAGAGAGGAGGTCAAG
GAAAAGTGAGTTCGGATCTAATATTCTCCCCGGTTTCCATTGAACAAGCG
TTTATTGGGGGCAGGGAAGAGGGTGCAGTCGGCTACACGTTTGTTCTTGT
CCAATCGCAACTCGCAACCAAACAACACAAACGCAAGATGGCCGGCAGAG
GCCGAGCCATACCATGTTGAACAAACAGTCTTCAAGTTTGGAATCTGGAA
CACATTTTCACGATGATTGGTAGAAAAAAATTTCACCCTGAACAGGCTTG
CCAAGTGTCGGTCGCCGGCTATGCGGGAGTCTGCAGCTCCGCCCGCAGCG
CGTAGCCGAATTTTGTAGCTGTTCATGTGGCCCACTTTTCGTTTTCCGGA
CAGCTGTTGTAAATGCCTCACACGCCCTCTAGGTGTTTCGTCGGCATGTC
TGGTACTCGGAAGTTTGTTCAGCGTTGGTCTGACGTACTCTTAGACGCCA
TTGCTGTTGTGGGGCTGGTCTAGTCCGACCTTGTGGTGCTTGTGCTTGGA
ACGACGCCCTTTCGTCGTTGCAGGACAGAGTCGATCATCGTGGACATTCT
GGTCGGGGAGAAGAGCAATCCCGCCCTCAGGGACAGCTTCGAGAACATGC
TCAAGAACGGAATGCTGGAAGACATGAAGAAAATGAAGGTGAAGCAGCGT
TGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTG
GTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGCGGTGTTGTTGTT
GGTGCTGCTGCTGCTGCTGCTGTTTTGTTGTGTTTCTCGTTGTTCTTGCT
CTTGTTCTTGTTGCTGTGCTGTTGCTGGTGGTCGTGGTCGTGGTCGTGGT
GTTGTGTTACGTGGTGTCGGTGATGGCCGTTGTCACAACTTGCCTTGACC
CCGTGTTTGCGCTTTGTACGGCGGTGAGCGGTGGGGTTGACGAAGAAATG
ATAGTCGCGCTCGCCCGTTCGTGCTTCGCGGTGTACGATTTCTCTCAGCG
CGTTCCCAATTCATGTTCACTGCTGTCCGACCACGTGCAGTATGTTCCGC
AATACAAATGTCCAGTAGCCAGTGGGTGCCAAAGGAAGCTCCCTTTCATC
CGAAAACCCTATTGAAATCCACGATACCAACCCTTTTGTTCTCTCTCTCT
CTCTCGTTTGCTTACCGTCCTCTCCCGACCAGGTTCCCTTAAATCGCAGC
AACACCCCTGACATGCCGGGCGTGATCCTCTCTGACAACACCCGCTTCAG
CATGCCCAAG
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Coding sequence (CDS) from alignment at P-littoralis_Contig669:10547..12406+

>mRNA_P-littoralis_Contig669.1.1 ID=mRNA_P-littoralis_Contig669.1.1|Name=mRNA_P-littoralis_Contig669.1.1|organism=Pylaiella littoralis U1_48|type=CDS|length=375bp|location=Sequence derived from alignment at P-littoralis_Contig669:10547..12406+ (Pylaiella littoralis U1_48)
ATGGTGTTGCTGTTGTTGTTGTTGTTGTTGATGTTGTTCATGAAGGTGGA
GGCGACAAAGTTCACTGAGGTCGGGTTTCACGGCCAGGACGTGGACCGGA
TCATCAAGGATCTTGTAGAGGTGGGCATCACCATGACGAAAAAGAAGCAG
ATGGAGGAGCACAGAGAGGAGGTCAAGGAAAAGACAGAGTCGATCATCGT
GGACATTCTGGTCGGGGAGAAGAGCAATCCCGCCCTCAGGGACAGCTTCG
AGAACATGCTCAAGAACGGAATGCTGGAAGACATGAAGAAAATGAAGGTT
CCCTTAAATCGCAGCAACACCCCTGACATGCCGGGCGTGATCCTCTCTGA
CAACACCCGCTTCAGCATGCCCAAG
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