prot_P-littoralis_Contig609.46.1 (polypeptide) Pylaiella littoralis U1_48

BLAST of mRNA_P-littoralis_Contig609.46.1 vs. uniprot
Match: A0A6H5K6L9_9PHAE (DDE-1 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K6L9_9PHAE)

HSP 1 Score: 259 bits (663), Expect = 1.790e-80
Identity = 151/347 (43.52%), Postives = 196/347 (56.48%), Query Frame = 0

Query:    4 FQKRFNAWYYRFPKRNRFSIRRKTSVGQKKPEGWEGKAWAFVQKLRAILREHAQDA------------------------------IFALLYNMNQTPIQAEMPSETTLEKTGAKSACITTGGEAEKLRYTVVLTVRKPGK-----------------------------NSISYEHLPEKRTSFDYPAGGISLGVQESSWCDGVQSDEWVTQDSMRRPGY-TLRDQRPSVLVLDDFRCHREEKFQQKLKDLANTLVVMLGGGLTPVCQPLDRNINKEFKRGFRARYTIWIRDNFG--RSGEKVSAPSRGLVATWVKEVWEGISERTIPSCFKVCGLTLNLDGSEDH 288
            F+ +FN+WY RF KRN FSIRR+TSVGQK P+G EG AWA V KLR  L + A +                               +F  L NM+QTP+Q E+P ETTLEKTGAK A I+TGG+ EK R+T+VL V   GK                             NS+++E  P  R    +PA G+S GVQE SW D  + + W++     RP   ++  QRP +LVLDDF+CH++E F   LK  ANT+V+ + G LTP+ QPLDR +NK+ KR  R +YT +        RSG K+  P RG+V+TW KE WE  +  T+ +CF +CGLTL LDGSEDH
Sbjct:   27 FKVKFNSWYQRFRKRNGFSIRRRTSVGQKLPKGDEGMAWATVMKLREALVKRAGEIYARRHPPAPDESPIKGEDLTSEQLASVEAEVFEELGNMDQTPVQHEIPVETTLEKTGAKDARISTGGK-EKERFTLVLAVMADGKKVSPRIIFKGTPFTPPTVSGKGKSTLPRKNSVAWEIHPANRAKHGHPANGMSFGVQEKSWSDQRECNGWISGSWKLRPNNGSIVQQRPCILVLDDFKCHKDEGFIAALKRDANTIVIFIPGRLTPLLQPLDRMLNKQMKRLLRRKYTAYSASAVADARSG-KLKPPERGVVSTWCKEAWESSTPETVKTCFMICGLTLALDGSEDH 371          

BLAST of mRNA_P-littoralis_Contig609.46.1 vs. uniprot
Match: A0A6H5JUK2_9PHAE (DDE-1 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JUK2_9PHAE)

HSP 1 Score: 257 bits (656), Expect = 5.310e-80
Identity = 156/376 (41.49%), Postives = 201/376 (53.46%), Query Frame = 0

Query:    4 FQKRFNAWYYRFPKRNRFSIRRKTSVGQKKPEGWEGKAWAFVQKLRAIL---------REH---------------------AQDAIFALLYNMNQTPIQAEMPSETTLEKTGAKSACITTGGEAEKLRYTVVLTVRKPGK-----------------------------NSISYEHLPEKRTSFDYPAGGISLGVQESSWCDGVQSDEWVTQDSMRRPGY-TLRDQRPSVLVLDDFRCHREEKFQQKLKDLANTLVVMLGGGLTPVCQPLDRNINKEFKRGFRARYTIWIRDNFG--RSGEKVSAPSRGLVATWVKEVWEGISERTIPS-----------------------------CFKVCGLTLNLDGSEDH 288
            F+ + N+WY RF KRN FSIRR+TSVGQK P+G+EG AWA V KLR  L         R H                      +  +F  L NM+QTP+Q EMP ETTLEKTGAK A I+TGG+ EK R+T+VL V   GK                             NS+++E  P  R   D+PA G+S GVQE SW D  + + W++     RP   ++  QRP +LVLDDF+CH++E +   LK  ANT+V+++ GGLTP+ QPLDR +NK+ KR  R +YT +        RSG K+  P RG+V+TW KE WE I+  T+ S                             CFK+CGLTL LDGSEDH
Sbjct:   27 FKVKINSWYQRFRKRNGFSIRRRTSVGQKLPKGYEGMAWATVMKLREALVRRAGEIYARRHPPAPDESPIKGEDLTFEQLASVEAEVFKELGNMDQTPVQHEMPVETTLEKTGAKDARISTGGK-EKERFTLVLAVMADGKKVSPRIIFKGTPFIPPAVSGKGKSTLPRKNSVAWEIHPANRAKHDHPANGMSFGVQEKSWSDQRECNGWISGSWKLRPNNGSIVQQRPCILVLDDFKCHKDEGYIAALKRDANTIVIVIPGGLTPLLQPLDRMLNKQMKRLLRGKYTAYSASAVADARSG-KLKPPERGVVSTWCKEAWESITPETVSSVRKERFALGDVRAWSGENPACSVXETVKTCFKICGLTLALDGSEDH 400          

BLAST of mRNA_P-littoralis_Contig609.46.1 vs. uniprot
Match: A0A6H5JHT8_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JHT8_9PHAE)

HSP 1 Score: 261 bits (668), Expect = 5.030e-79
Identity = 149/347 (42.94%), Postives = 195/347 (56.20%), Query Frame = 0

Query:    4 FQKRFNAWYYRFPKRNRFSIRRKTSVGQKKPEGWEGKAWAFVQKLRAILREHAQDA------------------------------IFALLYNMNQTPIQAEMPSETTLEKTGAKSACITTGGEAEKLRYTVVLTVR-----------------------------KPGKNSISYEHLPEKRTSFDYPAGGISLGVQESSWCDGVQSDEWVTQDSMRRPGY-TLRDQRPSVLVLDDFRCHREEKFQQKLKDLANTLVVMLGGGLTPVCQPLDRNINKEFKRGFRARYTIWIRDNFG--RSGEKVSAPSRGLVATWVKEVWEGISERTIPSCFKVCGLTLNLDGSEDH 288
            F+ +FN+WY RF KRN FSIRR+TSVGQK P+G+EG AWA V KL   L + A +                               +F  + NM+Q P+Q EMP ETTL+KTGAK A I+TGG  EK R+T+VL V                              +P KN +++E  P  R    +PA G+S GVQE SW D  + + W++     RP   ++  QRP +LVLDDF+CH +E F   LK  ANT+V+ + GGLTP+ QPLDR +NK+ KR  R +YT +        RSG K+  P RG+V+TW KE WE I+  T+ +C K+CGLTL LDGSEDH
Sbjct:  221 FKFKFNSWYQRFRKRNGFSIRRRTSVGQKLPKGYEGMAWATVMKLCEALVKRAGEIYARRHPPAPDESPIKGEDLNSEQLASVEAEVFEEVGNMDQMPVQHEMPVETTLDKTGAKDARISTGGGNEKERFTLVLAVTADGKKVSPRIIFEGTPFIPPTVSGKGKSTRPRKNPVAWEMHPANRAKHGHPANGMSFGVQEKSWSDQRECNGWISGSWKLRPNNGSIVQQRPRILVLDDFKCHNDEGFIAALKRDANTIVIFIPGGLTPLLQPLDRMLNKQMKRLLRGKYTAYSPSAVADARSG-KLKPPERGVVSTWCKEAWESITPETVKTCSKICGLTLALDGSEDH 566          

BLAST of mRNA_P-littoralis_Contig609.46.1 vs. uniprot
Match: A0A6H5JTG5_9PHAE (DDE-1 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JTG5_9PHAE)

HSP 1 Score: 257 bits (657), Expect = 5.040e-78
Identity = 151/355 (42.54%), Postives = 196/355 (55.21%), Query Frame = 0

Query:    4 FQKRFNAWYYRFPKRNRFSIRRKTSVGQKKPEGWEGKAWAFVQKLRAILREHAQDA------------------------------IFALLYNMNQTPIQAEMPSETTLEKTGAKSACITTGGEAEKLRYTVVLTVRKPGK-----------------------------NSISYEHLPEKRTSFDYPAGGISLGVQESSWCDGVQSDEWV--------TQDSMRRPGY-TLRDQRPSVLVLDDFRCHREEKFQQKLKDLANTLVVMLGGGLTPVCQPLDRNINKEFKRGFRARYTIWIRDNFG--RSGEKVSAPSRGLVATWVKEVWEGISERTIPSCFKVCGLTLNLDGSEDH 288
            F+ +FN+WY RF KRN FSIRR+ SVGQK P+G+EG AWA V KLR  L + A +                               +F  L NM+QTP+Q EMP ETTLE TG K A I+TGG+ EK R+T+VL V   GK                             NS+++E  P  R    +PA G+S GVQE SW    + + W+           SM RP   ++  QRP +LVLDDF+CH++E F   L   ANT+V+ + GGLTP+ QPLDR +NK+ KR  R +YT +        RSG K+  P RG+V+TW KE WE ++  T+ +CFK+CGLTL LDGSEDH
Sbjct:  181 FKVKFNSWYQRFRKRNGFSIRRRASVGQKLPKGYEGMAWATVTKLREALVKRAGEIYARRHPPAPDESPIKGEDLTSEQPASVEAEVFEELGNMDQTPVQHEMPVETTLEDTGGKDARISTGGK-EKERFTLVLAVMADGKMVSPCIIFKGTPFIPPTVSGKGKSTLPRKNSVAWEVHPANRAKHGHPANGMSFGVQEKSWSGQQECNGWIPGSWKLCPNNGSMXRPNNGSIVQQRPCILVLDDFKCHKDEGFIAALNRDANTIVIFIPGGLTPLLQPLDRMLNKQMKRLLRGKYTAYSAPAVADARSG-KLKPPERGVVSTWCKEAWEFVTSETVNTCFKICGLTLALDGSEDH 533          

BLAST of mRNA_P-littoralis_Contig609.46.1 vs. uniprot
Match: A0A6H5KE45_9PHAE (DDE-1 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KE45_9PHAE)

HSP 1 Score: 244 bits (624), Expect = 1.620e-73
Identity = 153/375 (40.80%), Postives = 193/375 (51.47%), Query Frame = 0

Query:    4 FQKRFNAWYYRFPKRNRFSIRRKTSVGQKKPEGWEGKAWAFVQKLRAILREHA------------------------------QDAIFALLYNMNQTPIQAEMPSETTLEKTGAKSACITTGGEA-------------------------EKLRYTVVLTVR-----------------------------KPGKNSISYEHLPEKRTSFDYPAGGISLGVQESSWCDGVQSDEWVTQDSMRRP--GYTLRDQRPSVLVLDDFRCHREEKFQQKLKDLANTLVVMLGGGLTPVCQPLDRNINKEFKRGFRARYTIWI----RDNFGRSGEKVSAPSRGLVATWVKEVWEGISERTIPSCFKVCGLTLNLDGSEDH 288
            F+ +F +WY RF KRN+FSIRR+TSVG + P+G EG AWA + KLR  L E A                               D +F  L NM+QTP+Q EMP ETTLEKTGAK A I TG                            EK R+T+ L V                              +P KNSI++E LP  R+    P GG+S GVQE SWCD  +   W+      RP  G  +R QR  +LVLDDF+CH+++ F   LK  ANT+V+ + GGLTP+ QPLDR +NK+ KR  R  YT       RD   ++G K++ P RG VATW K  W  I+  T+ +CFK+CGLTL LDGSEDH
Sbjct:  114 FKSKFKSWYQRFRKRNKFSIRRRTSVGIRLPKGHEGMAWATLMKLRKALLERAGEIYAQRYPPAPGESPIRGEDLSSSQLELVTDELFKDLGNMDQTPVQYEMPVETTLEKTGAKDARIATGAGVCCIRTILKPLPYPPTSCILPPPPGKEKERFTLCLAVSADGTKVPLRIIFKGTPFVPPATPGKGKATQPRKNSIAWEVLPANRSKHGLPVGGMSFGVQEKSWCDLREYKLWIKDSWKLRPNNGSVVR-QRRCILVLDDFKCHKDDGFIAALKREANTIVIFIPGGLTPLLQPLDRMLNKQMKRLLRGMYTAHTAKAARDP--KTG-KLAPPGRGAVATWCKNAWASITPETVKTCFKICGLTLALDGSEDH 484          

BLAST of mRNA_P-littoralis_Contig609.46.1 vs. uniprot
Match: A0A6H5JQM5_9PHAE (DDE-1 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQM5_9PHAE)

HSP 1 Score: 229 bits (584), Expect = 2.130e-67
Identity = 143/346 (41.33%), Postives = 187/346 (54.05%), Query Frame = 0

Query:    4 FQKRFNAWYYRFPKRNRFSIRRKTSVGQKKPEGWEGKAWAFVQKLRAILREHAQDA------------------------------IFALLYNMNQTPIQAEMPSETTLEKTGAKSACITTGGEAEKLRYTVVLTVR-----------------------------KPGKNSISYEHLPEKRTSFDYPAGGISLGVQESSWCDGVQSDEWVTQDSMRRPGY-TLRDQRPSVLVLDDFRCHREEKFQQKLKDLANTLVVMLGGGLTPVCQPLDRNINKEFKRGFRARYTIWIRDNFG--RSGEKVSAPSRGLVATWVKEVWEGISERTIPSCFKVCGLTLNLDGSED 287
            F+ +F  WY+RF +R+  SIRR+TSVGQK P G+EG A+A + KLR  L E A +                               +F  L NM+QTP+Q EMP ETTLEK GAK A I+TGG+ EK R+T+ L V                              +P K SI+ E LP  +T+F YP  G+S GVQE SW D  +   W+++    RP + ++  QR S+LVLDDFR               NT V+++ GGLTP+ QPLDR ++KE KR  R RYT ++       ++G K+  P RGLV+TW KE W  I+  T+  CFKVCGLTL LDGSED
Sbjct:  200 FRTKFKNWYHRFRERHGLSIRRRTSVGQKLPTGYEGLAFATLMKLRTALLERAGEIYARRNPPASGESPIKGKDLSPAQLESVATEVFEELGNMDQTPVQQEMPVETTLEKGGAKDARISTGGK-EKERFTLCLAVMADGGKVPLRIIFKGKPFIPPSTARRGKNTQPRKGSIAAEVLPANQTNFGYPVSGMSFGVQERSWYDACECTLWLSESWGFRPNHGSVIKQRHSILVLDDFRT--------------NTTVILIPGGLTPLLQPLDRMLDKEMKRLMRRRYTQYMASAIADPKTG-KLQPPGRGLVSTWCKESWAAITAETVKMCFKVCGLTLALDGSED 529          

BLAST of mRNA_P-littoralis_Contig609.46.1 vs. uniprot
Match: A0A6H5K8Y4_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K8Y4_9PHAE)

HSP 1 Score: 236 bits (603), Expect = 2.380e-67
Identity = 145/339 (42.77%), Postives = 183/339 (53.98%), Query Frame = 0

Query:    7 RFNAWYYRFPKRNRFSIRRKTSVGQKKPEGWEGKAWAFVQKLRAILREHA------------------------------QDAIFALLYNMNQTPIQAEMPSETTLEKTGAKSACITTGGEAEKLRYTVVLTVR-----------------------------KPGKNSISYEHLPEKRTSFDYPAGGISLGVQESSWCDGVQSDEWVTQDSMRRP--GYTLRDQRPSVLVLDDFRCHREEKFQQKLKDLANTLVVMLGGGLTPVCQPLDRNINKEFKRGFRARYTIWI----RDNFGRSGEKVSAPSRGLVATWVKEVWEGISERTIPSCFKVCGLTL 280
            +F +WY RF KRN+FSIRR+TSVGQK P+G EG AWA + KLR  L E A                               D +F  L NM+QTP+Q EMP ETTLE TGAK A I TGG+ EK R+T+ L V                              +P KNSI++E LP  R+    P GG+S GVQE SWCD  +   W+      RP  G  +R QR  +LVLDDF+CH+++ F   LK  ANT+V+ + GGL P+ QPLDR +NK+ KR  R  YT       RD   ++G K+  P RG VATW K  W  I+   + +CFK+CGLTL
Sbjct:  184 KFKSWYQRFCKRNKFSIRRRTSVGQKLPKGHEGMAWATLMKLRKALLERAGEIYAQRYPPAPGESPIRGEDLSSSQLELVTDELFEELGNMDQTPVQHEMPVETTLENTGAKDARIATGGK-EKERFTLCLAVSADGKKVPLRIIFKGTPFVPPATPGKGKATQPRKNSIAWEVLPANRSKHGLPVGGMSFGVQEKSWCDLRECKLWIKDSWKLRPNNGSIVR-QRRCILVLDDFKCHKDDGFIAALKREANTIVIFIPGGLIPLFQPLDRMLNKQMKRLLRGMYTAHTAKAARDP--KTG-KLVPPGRGAVATWCKNAWASITPEIVKTCFKICGLTL 517          

BLAST of mRNA_P-littoralis_Contig609.46.1 vs. uniprot
Match: A0A6H5KVR1_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KVR1_9PHAE)

HSP 1 Score: 229 bits (584), Expect = 4.250e-67
Identity = 137/327 (41.90%), Postives = 178/327 (54.43%), Query Frame = 0

Query:    4 FQKRFNAWYYRFPKRNRFSIRRKTSVGQKKPEGWEGKAWAFVQKLRAILREHA------------------------------QDAIFALLYNMNQTPIQAEMPSETTLEKTGAKSACITTGGEAEKLRYTVVLTVRKPGK--------NSISYEHLPEKRTSFDYPAGGISLGVQESSWCDGVQSDEWVTQDSMRRPGYTLRDQRPSVLVLDDFRCHREEKFQQKLKDLANTLVVMLGGGLTPVCQPLDRNINKEFKRGFRARYTIW----IRDNFGRSGEKVSAPSRGLVATWVKEVWEGISERTIPSCFKVCGLTLNLDGSEDH 288
            F+ +F +WY RF KRN+ SIRR+ SV QK P+G EG AW  + KLR  L E A                               D +F  L NM+QTP+Q EMP ETTLEKTG K A I TGG A   + T  ++   PGK        NSI++E LP  R+    P GG+S GVQE SWCD  +   W+      +  + LR    S+       CH+++ F   LK  A+T+V+ + GGLTP+ QPLDR +NK+  R  R  YT      +RD   ++G K++ P RG VATW K  W  I+  T+ +CFK+CGLTL LDGSEDH
Sbjct:  234 FKSKFKSWYQRFRKRNKLSIRRRISVDQKLPKGHEGMAWTTLMKLRKALLERAGEIYAQRYPPAPGESPIRGEDLSSSQLELVTDELFEELGNMDQTPVQHEMPVETTLEKTGVKDARIATGGPAAHFKGTPFVSPATPGKGKATQPRKNSIAWEVLPANRSKHGLPVGGMSFGVQEKSWCDLRECKLWI------KDSWELRPNNGSI-------CHKDDGFIAALKREASTIVIFIPGGLTPLLQPLDRMLNKQ--RLLRGMYTAHTAKAVRDP--KTG-KLAPPGRGAVATWCKNAWASITPETVKTCFKICGLTLALDGSEDH 542          

BLAST of mRNA_P-littoralis_Contig609.46.1 vs. uniprot
Match: A0A6H5KII4_9PHAE (DDE-1 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KII4_9PHAE)

HSP 1 Score: 221 bits (563), Expect = 6.840e-66
Identity = 117/259 (45.17%), Postives = 154/259 (59.46%), Query Frame = 0

Query:   60 IFALLYNMNQTPIQAEMPSETTLEKTGAKSACITTGGEAEKLRYTVVLTVRKPGK-----------------------------NSISYEHLPEKRTSFDYPAGGISLGVQESSWCDGVQSDEWVTQDSMRRPGY-TLRDQRPSVLVLDDFRCHREEKFQQKLKDLANTLVVMLGGGLTPVCQPLDRNINKEFKRGFRARYTIWIRDNFGRSGEKVSAPSRGLVATWVKEVWEGISERTIPSCFKVCGLTLNLDGSEDH 288
            +F  L NM+QTP+Q EMP ETTLEKTGAK A ++TGGE EK R+T+VL V   GK                             NS+++E  P  R    +PA G+S GVQE SW D  + + W +     RP   ++  QRP +LVLDDF+CH++E F   LK  ANT+V+ + GGLTP+ QPLDR +NK+ KR  R++YT +          K+  P RG+V+TW K  WE I+  T+ +CFK+CGLTL LDGSEDH
Sbjct:   47 VFEDLGNMDQTPVQHEMPVETTLEKTGAKDARVSTGGE-EKERFTLVLAVTADGKKVSLRIIFMGTPFIPPTVSGKGKSTLPRKNSVAWEMHPTNRAKHGHPANGMSFGVQEKSWSDQRECNGWTSGSWKLRPNNGSIVQQRPCILVLDDFKCHKDEGFIAALKRDANTIVIFIPGGLTPLLQPLDRMLNKQTKRLLRSKYTAYSASATDARSGKLKPPERGVVSTWCKGAWESITPETVKTCFKICGLTLALDGSEDH 304          

BLAST of mRNA_P-littoralis_Contig609.46.1 vs. uniprot
Match: A0A6H5KRZ8_9PHAE (DDE-1 domain-containing protein n=3 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KRZ8_9PHAE)

HSP 1 Score: 226 bits (576), Expect = 1.090e-65
Identity = 148/360 (41.11%), Postives = 191/360 (53.06%), Query Frame = 0

Query:    4 FQKRFNAWYYRFPKRNRFSIRRKTSVGQKKPEGWEGKAWAFVQKLRAILREHAQDA------------------------------IFALLYNMNQTPIQAEMPSETTLEKTGAKSACITTGGEAEKLRYTVVLTVRKPGKN---------------SISYE---HLPEKRT------SFDYPAGGISLGVQ------------------ESSWCDGVQSDEWVTQDSMRRPGY-TLRDQRPSVLVLDDFRCHREEKFQQKLKDLANTLVVMLGGGLTPVCQPLDRNINKEFKRGFRARYTIWIRDNFG--RSGEKVSAPSRGLVATWVKEVWEGISERTIPSCFKVCGLTLNLDGSEDH 288
            F+ +FN+WY RF KRN FSIRR+TSVGQK P+G+EG AWA V KLR  L + A +                               +F  L NM+QTP+Q EMP ETTLEKTGAK A I+TGGE EK R+T+VL V   GK                ++S +    LP K +      S +    G   G Q                  E SW D  + + W+      RP   ++  QRP +LVLDDF+CH++E F   LK  A T V+ + GGLT + QPLDR +NK+ KR  R +YT          RSG K++ P  G+V+TW KE WE I+ +T+ +CFK+CGLTL LDGSE H
Sbjct:  209 FKVKFNSWYQRFRKRNGFSIRRRTSVGQKLPKGYEGMAWATVMKLREALVKRAGEIYARRHPPAPDESPIKGEDLTSEQLASVEAEVFEELGNMDQTPVQHEMPVETTLEKTGAKDARISTGGE-EKERFTLVLAVMADGKKVSPRIIFKGTPFIPPTVSGKVKSTLPRKNSVAWEIHSANXARSGCHGGRQNDVSPRIIFKGTPFIPPTEKSWSDQRECNGWIPGSWKLRPNNGSIVQQRPCILVLDDFKCHKDEGFIAALKRGAKT-VIFIPGGLTSLLQPLDRMLNKQMKRLLRGKYTACSASAVADARSG-KLNPPEHGVVSTWCKEAWESITSKTVETCFKICGLTLALDGSEHH 565