mRNA_P-wetherbeei_contig54403.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig54403.1.1
Unique NamemRNA_P-wetherbeei_contig54403.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig54403.1.1 vs. uniprot
Match: UPI001BABF1AB (DDE-type integrase/transposase/recombinase n=2 Tax=Proteobacteria TaxID=1224 RepID=UPI001BABF1AB)

HSP 1 Score: 353 bits (905), Expect = 1.040e-117
Identity = 172/176 (97.73%), Postives = 173/176 (98.30%), Query Frame = 1 
Query:    1 LSLIQIDHTLVDVIVVDSETRAPIQRPWLTLAIDVCTRCVAGFHLSLEPPSATSVALCLSHGALAKDSWLAERRIDAVWPVRGIPERLHLDNAKEFRSEALKRGCEQYGIAIDHRPVRTPHYGGHIERLIGTMMGKVHLLPGTTFSNLQAKGDLIPDKTAAMTLEEVERWLGHAIA 528
            LSLIQIDHTLVDVIVVDSETR  IQRPWLTLAIDVCTRCVAGFHLSLEPPSATSVALCLSHGALAKDSWLAERRIDAVWPVRGIPERLHLDNAKEFRSEALKRGCEQYGIAIDHRPVRTPHYGGHIERLIGTMMGKVHLLPGTTFSN+QAKGDL PDKTAAMTLEEVERWLGHAIA
Sbjct:  169 LSLIQIDHTLVDVIVVDSETRTSIQRPWLTLAIDVCTRCVAGFHLSLEPPSATSVALCLSHGALAKDSWLAERRIDAVWPVRGIPERLHLDNAKEFRSEALKRGCEQYGIAIDHRPVRTPHYGGHIERLIGTMMGKVHLLPGTTFSNVQAKGDLNPDKTAAMTLEEVERWLGHAIA 344
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BLAST of mRNA_P-wetherbeei_contig54403.1.1 vs. uniprot
Match: A0A1Q5SWM5_9BRAD (Integrase n=2 Tax=unclassified Bradyrhizobium TaxID=2631580 RepID=A0A1Q5SWM5_9BRAD)

HSP 1 Score: 342 bits (878), Expect = 1.310e-113
Identity = 166/176 (94.32%), Postives = 171/176 (97.16%), Query Frame = 1 
Query:    1 LSLIQIDHTLVDVIVVDSETRAPIQRPWLTLAIDVCTRCVAGFHLSLEPPSATSVALCLSHGALAKDSWLAERRIDAVWPVRGIPERLHLDNAKEFRSEALKRGCEQYGIAIDHRPVRTPHYGGHIERLIGTMMGKVHLLPGTTFSNLQAKGDLIPDKTAAMTLEEVERWLGHAIA 528
            LSLIQIDHTLVDVIVVDS TR PIQRPWLTLAIDVCTRCVAGFHLSLEPPSATSVALCLSH AL+K+SWLAER IDAVWPVRGIPERLHLDNAKEFRSEALKRGCEQYGIAIDHRPVRTPHYGGHIERLIGT+MGKVHLLPGTTFSN+QAKGDL PDKTAAMTL+EVERWLGHAIA
Sbjct:  169 LSLIQIDHTLVDVIVVDSVTRMPIQRPWLTLAIDVCTRCVAGFHLSLEPPSATSVALCLSHAALSKESWLAERGIDAVWPVRGIPERLHLDNAKEFRSEALKRGCEQYGIAIDHRPVRTPHYGGHIERLIGTVMGKVHLLPGTTFSNVQAKGDLNPDKTAAMTLDEVERWLGHAIA 344
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BLAST of mRNA_P-wetherbeei_contig54403.1.1 vs. uniprot
Match: A0A2G1YED3_9SPHN (Integrase n=1 Tax=Sphingobium sp. TaxID=1912891 RepID=A0A2G1YED3_9SPHN)

HSP 1 Score: 340 bits (873), Expect = 7.000e-113
Identity = 163/176 (92.61%), Postives = 171/176 (97.16%), Query Frame = 1 
Query:    1 LSLIQIDHTLVDVIVVDSETRAPIQRPWLTLAIDVCTRCVAGFHLSLEPPSATSVALCLSHGALAKDSWLAERRIDAVWPVRGIPERLHLDNAKEFRSEALKRGCEQYGIAIDHRPVRTPHYGGHIERLIGTMMGKVHLLPGTTFSNLQAKGDLIPDKTAAMTLEEVERWLGHAIA 528
            LSLIQIDHTLVDVIVVDSETRAPIQRPWLTLAIDVCTRCVAGFHLSLEPPSATSVALCL+H AL K+SWLAER IDAVWPVRG+PERLHLDNAKEFRSEALKRGCEQYGIAIDHRPVRTPHYGGHIERLIGTMMGKVHLLPGTTFSN++ KGDL P+K+AAMTL+EVERWLGHAIA
Sbjct:  169 LSLIQIDHTLVDVIVVDSETRAPIQRPWLTLAIDVCTRCVAGFHLSLEPPSATSVALCLAHAALGKESWLAERGIDAVWPVRGVPERLHLDNAKEFRSEALKRGCEQYGIAIDHRPVRTPHYGGHIERLIGTMMGKVHLLPGTTFSNIREKGDLNPEKSAAMTLDEVERWLGHAIA 344
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BLAST of mRNA_P-wetherbeei_contig54403.1.1 vs. uniprot
Match: A0A521QEE9_9HYPH (Integrase n=2 Tax=Hyphomicrobiales TaxID=356 RepID=A0A521QEE9_9HYPH)

HSP 1 Score: 340 bits (872), Expect = 1.020e-112
Identity = 165/176 (93.75%), Postives = 172/176 (97.73%), Query Frame = 1 
Query:    1 LSLIQIDHTLVDVIVVDSETRAPIQRPWLTLAIDVCTRCVAGFHLSLEPPSATSVALCLSHGALAKDSWLAERRIDAVWPVRGIPERLHLDNAKEFRSEALKRGCEQYGIAIDHRPVRTPHYGGHIERLIGTMMGKVHLLPGTTFSNLQAKGDLIPDKTAAMTLEEVERWLGHAIA 528
            LSLIQIDHTLVDVIVVDSETRAPIQRPWLTLAIDVCTRCVAGFHLSLEPPSATSVALCLSH ALAK+SWLAER IDAVWPVRGIPERLHLDNAKEFRSEALKRGCEQYG+AID+RPVRTPHYGGHIERLIGTMMGKVHLLPGTTFS+++AK DL PDKTAAMTL+EVERWLGHAIA
Sbjct:  169 LSLIQIDHTLVDVIVVDSETRAPIQRPWLTLAIDVCTRCVAGFHLSLEPPSATSVALCLSHVALAKESWLAERGIDAVWPVRGIPERLHLDNAKEFRSEALKRGCEQYGVAIDYRPVRTPHYGGHIERLIGTMMGKVHLLPGTTFSDVRAKSDLNPDKTAAMTLDEVERWLGHAIA 344
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BLAST of mRNA_P-wetherbeei_contig54403.1.1 vs. uniprot
Match: M5EYS5_9HYPH (Integrase, catalytic region n=4 Tax=Hyphomicrobiales TaxID=356 RepID=M5EYS5_9HYPH)

HSP 1 Score: 338 bits (868), Expect = 3.970e-112
Identity = 162/176 (92.05%), Postives = 171/176 (97.16%), Query Frame = 1 
Query:    1 LSLIQIDHTLVDVIVVDSETRAPIQRPWLTLAIDVCTRCVAGFHLSLEPPSATSVALCLSHGALAKDSWLAERRIDAVWPVRGIPERLHLDNAKEFRSEALKRGCEQYGIAIDHRPVRTPHYGGHIERLIGTMMGKVHLLPGTTFSNLQAKGDLIPDKTAAMTLEEVERWLGHAIA 528
            LSLIQIDHTLVDV+VVDSETRAPIQRPWLTLAIDVCTRCVAGFHLSLEPPSATSVALCL+H ALAK+SWLAER IDA WPVRGIPERLHLDNAKEFRSEALKRGCEQYGIAIDHRPVRTPHYGGHIERLIGTMMGKVHLLPGTTFS++Q KGDL P+K+AAMT++EVERWLGHAIA
Sbjct:  169 LSLIQIDHTLVDVVVVDSETRAPIQRPWLTLAIDVCTRCVAGFHLSLEPPSATSVALCLTHAALAKESWLAERAIDAEWPVRGIPERLHLDNAKEFRSEALKRGCEQYGIAIDHRPVRTPHYGGHIERLIGTMMGKVHLLPGTTFSSVQEKGDLNPEKSAAMTIDEVERWLGHAIA 344
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BLAST of mRNA_P-wetherbeei_contig54403.1.1 vs. uniprot
Match: A0A7W5Z7L8_9HYPH (Putative transposase n=3 Tax=Hyphomicrobiales TaxID=356 RepID=A0A7W5Z7L8_9HYPH)

HSP 1 Score: 336 bits (862), Expect = 3.280e-111
Identity = 162/176 (92.05%), Postives = 172/176 (97.73%), Query Frame = 1 
Query:    1 LSLIQIDHTLVDVIVVDSETRAPIQRPWLTLAIDVCTRCVAGFHLSLEPPSATSVALCLSHGALAKDSWLAERRIDAVWPVRGIPERLHLDNAKEFRSEALKRGCEQYGIAIDHRPVRTPHYGGHIERLIGTMMGKVHLLPGTTFSNLQAKGDLIPDKTAAMTLEEVERWLGHAIA 528
            LSLIQIDHTLVDVIVVDSETRAPIQRPWLTLAIDVC+RCVAGFHLSLEPPSATSVALCL+H AL+K+SWLAER IDA WPVRGIPERLHLDNAKEFRSEALKRGCEQYGIAID+RPVRTPHYGGHIERLIGTMMGKVHLLPGTTFS+++AKGDL P+KTAAMTL+EVERWLGHAIA
Sbjct:  169 LSLIQIDHTLVDVIVVDSETRAPIQRPWLTLAIDVCSRCVAGFHLSLEPPSATSVALCLTHAALSKESWLAERGIDAEWPVRGIPERLHLDNAKEFRSEALKRGCEQYGIAIDYRPVRTPHYGGHIERLIGTMMGKVHLLPGTTFSDVRAKGDLDPEKTAAMTLDEVERWLGHAIA 344
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BLAST of mRNA_P-wetherbeei_contig54403.1.1 vs. uniprot
Match: UPI0018C2D031 (DDE-type integrase/transposase/recombinase n=2 Tax=Alphaproteobacteria TaxID=28211 RepID=UPI0018C2D031)

HSP 1 Score: 336 bits (862), Expect = 3.280e-111
Identity = 162/176 (92.05%), Postives = 170/176 (96.59%), Query Frame = 1 
Query:    1 LSLIQIDHTLVDVIVVDSETRAPIQRPWLTLAIDVCTRCVAGFHLSLEPPSATSVALCLSHGALAKDSWLAERRIDAVWPVRGIPERLHLDNAKEFRSEALKRGCEQYGIAIDHRPVRTPHYGGHIERLIGTMMGKVHLLPGTTFSNLQAKGDLIPDKTAAMTLEEVERWLGHAIA 528
            LSLIQIDHTLVDVIVVDSETRAPIQRPWLTLAIDVCTRCVAGFHLSLEPPSATSVALCLSH AL+K+SWLAER IDA WPVRGIPERLHLDNAKEFRSEALKRGCEQYGIAID+RPVRTPHYGGHIERLIGTMMGKVHLLPGTTFS++Q +GDL P KTAAMTL+EVERWLGHA+A
Sbjct:  169 LSLIQIDHTLVDVIVVDSETRAPIQRPWLTLAIDVCTRCVAGFHLSLEPPSATSVALCLSHAALSKESWLAERGIDAEWPVRGIPERLHLDNAKEFRSEALKRGCEQYGIAIDYRPVRTPHYGGHIERLIGTMMGKVHLLPGTTFSDVQERGDLDPQKTAAMTLDEVERWLGHAVA 344
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BLAST of mRNA_P-wetherbeei_contig54403.1.1 vs. uniprot
Match: A0A2A4FPD1_9SPHN (Integrase n=1 Tax=Rhizorhabdus dicambivorans TaxID=1850238 RepID=A0A2A4FPD1_9SPHN)

HSP 1 Score: 336 bits (862), Expect = 3.680e-111
Identity = 160/176 (90.91%), Postives = 170/176 (96.59%), Query Frame = 1 
Query:    1 LSLIQIDHTLVDVIVVDSETRAPIQRPWLTLAIDVCTRCVAGFHLSLEPPSATSVALCLSHGALAKDSWLAERRIDAVWPVRGIPERLHLDNAKEFRSEALKRGCEQYGIAIDHRPVRTPHYGGHIERLIGTMMGKVHLLPGTTFSNLQAKGDLIPDKTAAMTLEEVERWLGHAIA 528
            LSLIQIDHTLVDVIVVDSETRAPIQRPWLTLAIDVCTRCVAGFHLSLEPPSATSVA+CL+H AL K++WLA R IDAVWPVRG+PERLHLDNAKEFRSEALKRGCEQYGIAIDHRPVRTPHYGGHIERLIGTMMGKVHLLPGTTFSN++ KGDL P+K+AAMTL+EVERWLGHAIA
Sbjct:  169 LSLIQIDHTLVDVIVVDSETRAPIQRPWLTLAIDVCTRCVAGFHLSLEPPSATSVAMCLAHAALGKETWLATRDIDAVWPVRGVPERLHLDNAKEFRSEALKRGCEQYGIAIDHRPVRTPHYGGHIERLIGTMMGKVHLLPGTTFSNIREKGDLNPEKSAAMTLDEVERWLGHAIA 344
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BLAST of mRNA_P-wetherbeei_contig54403.1.1 vs. uniprot
Match: A0A6N7H5F5_9HYPH (DDE-type integrase/transposase/recombinase (Fragment) n=2 Tax=Hyphomicrobiales TaxID=356 RepID=A0A6N7H5F5_9HYPH)

HSP 1 Score: 330 bits (847), Expect = 4.130e-109
Identity = 161/176 (91.48%), Postives = 168/176 (95.45%), Query Frame = 1 
Query:    1 LSLIQIDHTLVDVIVVDSETRAPIQRPWLTLAIDVCTRCVAGFHLSLEPPSATSVALCLSHGALAKDSWLAERRIDAVWPVRGIPERLHLDNAKEFRSEALKRGCEQYGIAIDHRPVRTPHYGGHIERLIGTMMGKVHLLPGTTFSNLQAKGDLIPDKTAAMTLEEVERWLGHAIA 528
            LSLIQIDHTLVDVIVVDS TR PIQRPWLTLAIDVCTRCVAGFHLSLEPPSATSVALCLSH  L K+SWLAER IDAVWPVRGIPERLHLDNAKEFRSEALKRGCEQYGIAID+RPVRTPHYGGHIERLIGTMMGKVHLLPGTTFS+++AKG+L P KTAAMTL+EVERWLGHAIA
Sbjct:  156 LSLIQIDHTLVDVIVVDSVTRTPIQRPWLTLAIDVCTRCVAGFHLSLEPPSATSVALCLSHVVLTKESWLAERGIDAVWPVRGIPERLHLDNAKEFRSEALKRGCEQYGIAIDYRPVRTPHYGGHIERLIGTMMGKVHLLPGTTFSDIRAKGNLNPYKTAAMTLDEVERWLGHAIA 331
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BLAST of mRNA_P-wetherbeei_contig54403.1.1 vs. uniprot
Match: A0A2S0QYG0_9RHOO (Integrase n=5 Tax=Proteobacteria TaxID=1224 RepID=A0A2S0QYG0_9RHOO)

HSP 1 Score: 326 bits (836), Expect = 2.710e-107
Identity = 156/176 (88.64%), Postives = 164/176 (93.18%), Query Frame = 1 
Query:    1 LSLIQIDHTLVDVIVVDSETRAPIQRPWLTLAIDVCTRCVAGFHLSLEPPSATSVALCLSHGALAKDSWLAERRIDAVWPVRGIPERLHLDNAKEFRSEALKRGCEQYGIAIDHRPVRTPHYGGHIERLIGTMMGKVHLLPGTTFSNLQAKGDLIPDKTAAMTLEEVERWLGHAIA 528
            LSL+QIDHTLVDVIVVDS+TR PIQRPWLTLAIDVCTRCVAG HLSLEPPSATSVALC+S  AL KD+WLAER ID  WPV GIPERLHLDNAKEFRSEAL+RGCEQ+GIAIDHRPVRTPHYGGHIERLIGTMMGKVHLLPGTTFSN+QAKGDL P K+A MTLEEVERWLGHAIA
Sbjct:  170 LSLVQIDHTLVDVIVVDSQTREPIQRPWLTLAIDVCTRCVAGLHLSLEPPSATSVALCISQAALPKDAWLAERGIDGAWPVHGIPERLHLDNAKEFRSEALRRGCEQHGIAIDHRPVRTPHYGGHIERLIGTMMGKVHLLPGTTFSNVQAKGDLDPSKSAVMTLEEVERWLGHAIA 345
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig54403.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UPI001BABF1AB1.040e-11797.73DDE-type integrase/transposase/recombinase n=2 Tax... [more]
A0A1Q5SWM5_9BRAD1.310e-11394.32Integrase n=2 Tax=unclassified Bradyrhizobium TaxI... [more]
A0A2G1YED3_9SPHN7.000e-11392.61Integrase n=1 Tax=Sphingobium sp. TaxID=1912891 Re... [more]
A0A521QEE9_9HYPH1.020e-11293.75Integrase n=2 Tax=Hyphomicrobiales TaxID=356 RepID... [more]
M5EYS5_9HYPH3.970e-11292.05Integrase, catalytic region n=4 Tax=Hyphomicrobial... [more]
A0A7W5Z7L8_9HYPH3.280e-11192.05Putative transposase n=3 Tax=Hyphomicrobiales TaxI... [more]
UPI0018C2D0313.280e-11192.05DDE-type integrase/transposase/recombinase n=2 Tax... [more]
A0A2A4FPD1_9SPHN3.680e-11190.91Integrase n=1 Tax=Rhizorhabdus dicambivorans TaxID... [more]
A0A6N7H5F5_9HYPH4.130e-10991.48DDE-type integrase/transposase/recombinase (Fragme... [more]
A0A2S0QYG0_9RHOO2.710e-10788.64Integrase n=5 Tax=Proteobacteria TaxID=1224 RepID=... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig54403contigP-wetherbeei_contig54403:3..530 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog1297569.MESS2_850019
Preferred nametniA
PFAMsHTH_28,HTH_Tnp_Mu_2,Mu-transpos_C,rve
Model size528
Max annot lvl28211|Alphaproteobacteria
KEGG koko:K07497
Hectar predicted targeting categoryother localisation
Exons1
Evalue6.51e-113
EggNOG OGsCOG2801@1|root,COG2801@2|Bacteria,1MXCK@1224|Proteobacteria,2TTWU@28211|Alphaproteobacteria,43M1V@69277|Phyllobacteriaceae
DescriptionMu transposase, C-terminal
Cds size528
COG categoryL
BRITEko00000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186813.033958-CDS-P-wetherbeei_contig54403:2..5301683186813.033958-CDS-P-wetherbeei_contig54403:2..530Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig54403 3..530 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig54403.1.1prot_P-wetherbeei_contig54403.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig54403 3..530 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig54403.1.1

>prot_P-wetherbeei_contig54403.1.1 ID=prot_P-wetherbeei_contig54403.1.1|Name=mRNA_P-wetherbeei_contig54403.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=176bp
LSLIQIDHTLVDVIVVDSETRAPIQRPWLTLAIDVCTRCVAGFHLSLEPP
SATSVALCLSHGALAKDSWLAERRIDAVWPVRGIPERLHLDNAKEFRSEA
LKRGCEQYGIAIDHRPVRTPHYGGHIERLIGTMMGKVHLLPGTTFSNLQA
KGDLIPDKTAAMTLEEVERWLGHAIA
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mRNA from alignment at P-wetherbeei_contig54403:3..530-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig54403.1.1 ID=mRNA_P-wetherbeei_contig54403.1.1|Name=mRNA_P-wetherbeei_contig54403.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=528bp|location=Sequence derived from alignment at P-wetherbeei_contig54403:3..530- (Phaeothamnion wetherbeei SAG_119_79)
CTGTCGCTGATCCAGATCGACCATACGCTGGTCGATGTAATTGTGGTTGA
CAGCGAAACCCGTGCGCCGATCCAGCGTCCGTGGCTGACGTTGGCGATCG
ACGTTTGCACGCGCTGCGTTGCCGGTTTCCATCTCTCGCTGGAACCACCG
TCTGCAACGTCGGTTGCGCTGTGTCTGAGCCACGGCGCGCTCGCCAAGGA
CAGTTGGCTTGCCGAACGACGCATCGATGCGGTGTGGCCAGTGCGGGGCA
TTCCGGAACGACTGCATCTGGACAATGCGAAGGAGTTCCGTTCGGAAGCT
CTGAAGCGCGGTTGCGAGCAATACGGGATCGCGATCGACCATAGGCCGGT
GCGAACACCGCACTATGGCGGCCATATCGAACGCCTGATCGGCACCATGA
TGGGCAAGGTTCATCTGCTGCCGGGAACGACGTTCTCCAATCTCCAGGCA
AAGGGCGATCTCATCCCCGACAAAACGGCAGCAATGACACTGGAGGAAGT
CGAGCGTTGGCTCGGTCATGCGATAGCA
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Coding sequence (CDS) from alignment at P-wetherbeei_contig54403:3..530-

>mRNA_P-wetherbeei_contig54403.1.1 ID=mRNA_P-wetherbeei_contig54403.1.1|Name=mRNA_P-wetherbeei_contig54403.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=528bp|location=Sequence derived from alignment at P-wetherbeei_contig54403:3..530- (Phaeothamnion wetherbeei SAG_119_79)
CTGTCGCTGATCCAGATCGACCATACGCTGGTCGATGTAATTGTGGTTGA
CAGCGAAACCCGTGCGCCGATCCAGCGTCCGTGGCTGACGTTGGCGATCG
ACGTTTGCACGCGCTGCGTTGCCGGTTTCCATCTCTCGCTGGAACCACCG
TCTGCAACGTCGGTTGCGCTGTGTCTGAGCCACGGCGCGCTCGCCAAGGA
CAGTTGGCTTGCCGAACGACGCATCGATGCGGTGTGGCCAGTGCGGGGCA
TTCCGGAACGACTGCATCTGGACAATGCGAAGGAGTTCCGTTCGGAAGCT
CTGAAGCGCGGTTGCGAGCAATACGGGATCGCGATCGACCATAGGCCGGT
GCGAACACCGCACTATGGCGGCCATATCGAACGCCTGATCGGCACCATGA
TGGGCAAGGTTCATCTGCTGCCGGGAACGACGTTCTCCAATCTCCAGGCA
AAGGGCGATCTCATCCCCGACAAAACGGCAGCAATGACACTGGAGGAAGT
CGAGCGTTGGCTCGGTCATGCGATAGCA
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