mRNA_P-wetherbeei_contig17994.2.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig17994.2.1
Unique NamemRNA_P-wetherbeei_contig17994.2.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig17994.2.1 vs. uniprot
Match: A0A1Y0G8E0_9PROT (Crossover junction endodeoxyribonuclease RuvC n=1 Tax=Sulfuriferula sp. AH1 TaxID=1985873 RepID=A0A1Y0G8E0_9PROT)

HSP 1 Score: 273 bits (698), Expect = 1.440e-91
Identity = 140/173 (80.92%), Postives = 154/173 (89.02%), Query Frame = 1 
Query:    7 SIRILGIDPGLRVTGFGVIDKVGTRLTYVTSGCVKSGEGELPERIKVLLEGLSEVIAANRPDQSAIEKVFVNVNPQSTLLLGQARGAAICAAVLAGLPVAEYTALQVKQSVVGAGKAAKEQVQMMVKRLLSLAGDPSPDAADALACAICHAHAGQGLGAFPTKGRRMKDGRLV 525
            +IRILGIDPGLRVTGFGV++K+G RL YVTSGC++SGEGELP+R+KV+L GL EVIA N+P Q AIEKVFVNVNPQSTLLLGQARGAAICAAVL  LPVAEYTALQVKQSVVG G AAK+QVQ MVKRLL+L G PSPDAADALACAICHAH GQGLGA  T G RMK+GRL+
Sbjct:    4 TIRILGIDPGLRVTGFGVLEKIGNRLVYVTSGCIRSGEGELPQRLKVILAGLGEVIAVNQPQQVAIEKVFVNVNPQSTLLLGQARGAAICAAVLHDLPVAEYTALQVKQSVVGNGHAAKDQVQEMVKRLLNLPGVPSPDAADALACAICHAHGGQGLGALATAGYRMKNGRLI 176
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BLAST of mRNA_P-wetherbeei_contig17994.2.1 vs. uniprot
Match: A0A401JFB9_9PROT (Crossover junction endodeoxyribonuclease RuvC n=2 Tax=Sulfuriferula TaxID=1778653 RepID=A0A401JFB9_9PROT)

HSP 1 Score: 268 bits (684), Expect = 1.830e-89
Identity = 138/174 (79.31%), Postives = 151/174 (86.78%), Query Frame = 1 
Query:    4 ESIRILGIDPGLRVTGFGVIDKVGTRLTYVTSGCVKSGEGELPERIKVLLEGLSEVIAANRPDQSAIEKVFVNVNPQSTLLLGQARGAAICAAVLAGLPVAEYTALQVKQSVVGAGKAAKEQVQMMVKRLLSLAGDPSPDAADALACAICHAHAGQGLGAFPTKGRRMKDGRLV 525
             SIRILGIDPGLR+TGFGVI+K G R+ Y+TSGC+KSGEGELPER+KV+L  L EVI  N+P Q A+EKVFVNVNPQSTLLLGQARGAAICAAVL  LPVAEYTALQVKQ+VVG G AAKEQVQ MVKRLL+L G PSPDAADALACAICHAH GQGLG F T G R+K+GRLV
Sbjct:    2 NSIRILGIDPGLRITGFGVIEKTGNRIQYLTSGCIKSGEGELPERLKVILLSLREVIQNNQPQQVAVEKVFVNVNPQSTLLLGQARGAAICAAVLEALPVAEYTALQVKQAVVGNGHAAKEQVQEMVKRLLNLPGVPSPDAADALACAICHAHGGQGLGGFATAGYRVKNGRLV 175
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BLAST of mRNA_P-wetherbeei_contig17994.2.1 vs. uniprot
Match: A0A7Y4S5U6_9PROT (Crossover junction endodeoxyribonuclease RuvC n=3 Tax=Sulfuriferula TaxID=1778653 RepID=A0A7Y4S5U6_9PROT)

HSP 1 Score: 267 bits (683), Expect = 2.600e-89
Identity = 137/173 (79.19%), Postives = 153/173 (88.44%), Query Frame = 1 
Query:    7 SIRILGIDPGLRVTGFGVIDKVGTRLTYVTSGCVKSGEGELPERIKVLLEGLSEVIAANRPDQSAIEKVFVNVNPQSTLLLGQARGAAICAAVLAGLPVAEYTALQVKQSVVGAGKAAKEQVQMMVKRLLSLAGDPSPDAADALACAICHAHAGQGLGAFPTKGRRMKDGRLV 525
            +IRILGIDPGLRVTGFGVIDK+G RL YVTSGC+KSGEGELP+R+ V+L GL EVI+ ++P Q+A+EKVFVNVNPQSTLLLGQARGAAICAAVL  LPVAEYTALQVKQ+VVG G AAKEQVQ MVKRLL+L G PS DAADALACAICHAH GQGLGA  T G R+K+GRL+
Sbjct:    2 TIRILGIDPGLRVTGFGVIDKIGNRLVYVTSGCIKSGEGELPQRLGVILAGLDEVISLHQPQQAAVEKVFVNVNPQSTLLLGQARGAAICAAVLKNLPVAEYTALQVKQAVVGNGHAAKEQVQEMVKRLLNLPGVPSSDAADALACAICHAHGGQGLGALATAGYRVKNGRLI 174
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BLAST of mRNA_P-wetherbeei_contig17994.2.1 vs. uniprot
Match: A0A1F4AHP4_9PROT (Crossover junction endodeoxyribonuclease RuvC n=1 Tax=Betaproteobacteria bacterium RIFCSPLOWO2_02_64_14 TaxID=1797482 RepID=A0A1F4AHP4_9PROT)

HSP 1 Score: 261 bits (667), Expect = 8.430e-87
Identity = 132/175 (75.43%), Postives = 150/175 (85.71%), Query Frame = 1 
Query:    1 AESIRILGIDPGLRVTGFGVIDKVGTRLTYVTSGCVKSGEGELPERIKVLLEGLSEVIAANRPDQSAIEKVFVNVNPQSTLLLGQARGAAICAAVLAGLPVAEYTALQVKQSVVGAGKAAKEQVQMMVKRLLSLAGDPSPDAADALACAICHAHAGQGLGAFPTKGRRMKDGRLV 525
            A ++RILGIDPGLR+TGFGVIDK G RLTYVTSGC+++  G L ER+K +L+GL EVIA  RP+Q A+EKVFVNVNPQSTLLLGQARG AICAAV+ GLPV+EYTALQVKQ+VVG G AAK+QVQ MVKRLL LAGDP+PDAADALACAICHAH GQG+G   T G RM+ GRLV
Sbjct:    6 APAVRILGIDPGLRITGFGVIDKTGQRLTYVTSGCIRTPAGALSERLKAILDGLDEVIAGCRPEQVALEKVFVNVNPQSTLLLGQARGTAICAAVMHGLPVSEYTALQVKQAVVGKGHAAKDQVQEMVKRLLKLAGDPNPDAADALACAICHAHGGQGMGRLATAGYRMRRGRLV 180
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BLAST of mRNA_P-wetherbeei_contig17994.2.1 vs. uniprot
Match: A0A1G0BXM2_9PROT (Crossover junction endodeoxyribonuclease RuvC n=3 Tax=Betaproteobacteria TaxID=28216 RepID=A0A1G0BXM2_9PROT)

HSP 1 Score: 261 bits (666), Expect = 1.420e-86
Identity = 132/171 (77.19%), Postives = 150/171 (87.72%), Query Frame = 1 
Query:   13 RILGIDPGLRVTGFGVIDKVGTRLTYVTSGCVKSGEGELPERIKVLLEGLSEVIAANRPDQSAIEKVFVNVNPQSTLLLGQARGAAICAAVLAGLPVAEYTALQVKQSVVGAGKAAKEQVQMMVKRLLSLAGDPSPDAADALACAICHAHAGQGLGAFPTKGRRMKDGRLV 525
            RILGIDPGLR+TGFG++DK G +L+YV SGC+K+ +GELPER+KV+L  LSEVIA +RPDQ+A+EKVFVNVNPQSTLLLGQARGAAICAAVLA LPVAEYTALQVKQ+VVG+G A KEQVQ MVKRLL L+G P  DAADALACAICHAH G GLGA  TKG R++ GRLV
Sbjct:   15 RILGIDPGLRITGFGILDKTGQQLSYVASGCIKTPDGELPERLKVILNSLSEVIAQHRPDQAAVEKVFVNVNPQSTLLLGQARGAAICAAVLANLPVAEYTALQVKQAVVGSGHAKKEQVQEMVKRLLKLSGTPGTDAADALACAICHAHGGLGLGALATKGFRVRHGRLV 185
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BLAST of mRNA_P-wetherbeei_contig17994.2.1 vs. uniprot
Match: A0A522X4L9_9PROT (Crossover junction endodeoxyribonuclease RuvC n=2 Tax=Nitrosomonadales TaxID=32003 RepID=A0A522X4L9_9PROT)

HSP 1 Score: 260 bits (665), Expect = 2.470e-86
Identity = 132/173 (76.30%), Postives = 151/173 (87.28%), Query Frame = 1 
Query:    4 ESIRILGIDPGLRVTGFGVIDKVGTRLTYVTSGCVKSGEGELPERIKVLLEGLSEVIAANRPDQSAIEKVFVNVNPQSTLLLGQARGAAICAAVLAGLPVAEYTALQVKQSVVGAGKAAKEQVQMMVKRLLSLAGDPSPDAADALACAICHAHAGQGLGAFPTKGRRMKDGRL 522
             +IRILGIDPGLRVTGFGV+DKVG +L YV SGC+K+ +GELPER+KV+L  L EVIA ++P Q A+EKVFVNVNPQSTLLLGQARGAAICAAVLA LPV+EYTALQVKQ+VVG G A KEQVQ+MV+RLL+L+G P PDAADALACAICHAH G GLGA  TKG RM++GRL
Sbjct:   18 STIRILGIDPGLRVTGFGVLDKVGQQLVYVASGCIKTPDGELPERLKVILNSLGEVIAQHQPGQVAVEKVFVNVNPQSTLLLGQARGAAICAAVLANLPVSEYTALQVKQAVVGNGHADKEQVQLMVQRLLNLSGTPGPDAADALACAICHAHGGTGLGALATKGMRMRNGRL 190
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BLAST of mRNA_P-wetherbeei_contig17994.2.1 vs. uniprot
Match: A0A1F3WY67_9PROT (Crossover junction endodeoxyribonuclease RuvC n=2 Tax=Betaproteobacteria TaxID=28216 RepID=A0A1F3WY67_9PROT)

HSP 1 Score: 259 bits (662), Expect = 5.760e-86
Identity = 130/172 (75.58%), Postives = 149/172 (86.63%), Query Frame = 1 
Query:   10 IRILGIDPGLRVTGFGVIDKVGTRLTYVTSGCVKSGEGELPERIKVLLEGLSEVIAANRPDQSAIEKVFVNVNPQSTLLLGQARGAAICAAVLAGLPVAEYTALQVKQSVVGAGKAAKEQVQMMVKRLLSLAGDPSPDAADALACAICHAHAGQGLGAFPTKGRRMKDGRLV 525
            +RILGIDPGLR+TGFGV+DKVG +L+YV SGC+K+ +GELPER+K +L+ L EVIA ++P Q A+EKVFVN+NPQSTL LGQARGAAICAAVLA LPVAEYTALQVKQ+VVG G A KEQVQ MVKRLL L+G PSPDAADALACA+CHAH GQGLG   T G RMK+GRLV
Sbjct:   14 VRILGIDPGLRITGFGVLDKVGQQLSYVASGCIKTPDGELPERLKAILDSLGEVIAQHQPQQVAVEKVFVNINPQSTLALGQARGAAICAAVLANLPVAEYTALQVKQAVVGNGHAKKEQVQEMVKRLLKLSGIPSPDAADALACAVCHAHGGQGLGVLATAGYRMKNGRLV 185
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BLAST of mRNA_P-wetherbeei_contig17994.2.1 vs. uniprot
Match: D9SCK9_GALCS (Crossover junction endodeoxyribonuclease RuvC n=11 Tax=Nitrosomonadales TaxID=32003 RepID=D9SCK9_GALCS)

HSP 1 Score: 257 bits (657), Expect = 3.940e-85
Identity = 132/173 (76.30%), Postives = 149/173 (86.13%), Query Frame = 1 
Query:    7 SIRILGIDPGLRVTGFGVIDKVGTRLTYVTSGCVKSGEGELPERIKVLLEGLSEVIAANRPDQSAIEKVFVNVNPQSTLLLGQARGAAICAAVLAGLPVAEYTALQVKQSVVGAGKAAKEQVQMMVKRLLSLAGDPSPDAADALACAICHAHAGQGLGAFPTKGRRMKDGRLV 525
            SIRILGIDPGLR+TGFGVIDKVG +L YV SGC+K+  G+LPER+KV+L  L  VI  ++PDQ+A+EKVFVNVNPQSTLLLGQARGAAICAAVLA LPV EYTALQVKQ+VVG G A KEQVQ+MV+RLL L+G PSPDAADALACAICHAH G GLGA  TKG R++ GRLV
Sbjct:   18 SIRILGIDPGLRITGFGVIDKVGQQLHYVASGCIKTPAGDLPERLKVILNSLGVVITQHQPDQAAVEKVFVNVNPQSTLLLGQARGAAICAAVLANLPVGEYTALQVKQAVVGNGHADKEQVQLMVQRLLKLSGTPSPDAADALACAICHAHGGLGLGALATKGFRVRGGRLV 190
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BLAST of mRNA_P-wetherbeei_contig17994.2.1 vs. uniprot
Match: A0A2Z6GBN2_9PROT (Crossover junction endodeoxyribonuclease RuvC n=9 Tax=Bacteria TaxID=2 RepID=A0A2Z6GBN2_9PROT)

HSP 1 Score: 256 bits (655), Expect = 4.620e-85
Identity = 132/171 (77.19%), Postives = 148/171 (86.55%), Query Frame = 1 
Query:   13 RILGIDPGLRVTGFGVIDKVGTRLTYVTSGCVKSGEGELPERIKVLLEGLSEVIAANRPDQSAIEKVFVNVNPQSTLLLGQARGAAICAAVLAGLPVAEYTALQVKQSVVGAGKAAKEQVQMMVKRLLSLAGDPSPDAADALACAICHAHAGQGLGAFPTKGRRMKDGRLV 525
            RILGIDPGLR+TGFGV+DK G +L+YV SGC+K+ +GELPER+KV+L  L EVIA ++P Q A+EKVFVNVNPQSTLLLGQARGAAICAAV A LPVAEYTALQVKQSVVG G AAKEQVQ MVKRLL+L+G PSPDAADALACAICHAH G GLG   TKG R+K GRL+
Sbjct:    4 RILGIDPGLRITGFGVLDKQGQQLSYVASGCIKTPDGELPERLKVILLSLGEVIALHQPQQVAVEKVFVNVNPQSTLLLGQARGAAICAAVSAHLPVAEYTALQVKQSVVGNGHAAKEQVQEMVKRLLNLSGTPSPDAADALACAICHAHGGMGLGGLATKGFRVKGGRLI 174
[Close]
BLAST of mRNA_P-wetherbeei_contig17994.2.1 vs. uniprot
Match: D5CMR6_SIDLE (Crossover junction endodeoxyribonuclease RuvC n=8 Tax=root TaxID=1 RepID=D5CMR6_SIDLE)

HSP 1 Score: 256 bits (655), Expect = 6.270e-85
Identity = 131/174 (75.29%), Postives = 149/174 (85.63%), Query Frame = 1 
Query:    4 ESIRILGIDPGLRVTGFGVIDKVGTRLTYVTSGCVKSGEGELPERIKVLLEGLSEVIAANRPDQSAIEKVFVNVNPQSTLLLGQARGAAICAAVLAGLPVAEYTALQVKQSVVGAGKAAKEQVQMMVKRLLSLAGDPSPDAADALACAICHAHAGQGLGAFPTKGRRMKDGRLV 525
             SIRILGIDPGLR+TGFGV+DK G +L YV SGC+K+ +GELPER+KV+L  L EVIA ++P+Q A+EKVFVNVNPQSTLLLGQARGAAICAAV A LPVAEYTALQVKQ+VVG G A KEQVQ MV+RLL L+G PSPDAADALACAICHAH G G+GA  TKG R++ GRLV
Sbjct:   10 SSIRILGIDPGLRITGFGVVDKEGQQLHYVASGCIKTPDGELPERLKVILASLGEVIAQHKPEQVAVEKVFVNVNPQSTLLLGQARGAAICAAVQADLPVAEYTALQVKQAVVGNGHADKEQVQAMVQRLLKLSGTPSPDAADALACAICHAHGGMGMGALATKGFRVRKGRLV 183
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig17994.2.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A1Y0G8E0_9PROT1.440e-9180.92Crossover junction endodeoxyribonuclease RuvC n=1 ... [more]
A0A401JFB9_9PROT1.830e-8979.31Crossover junction endodeoxyribonuclease RuvC n=2 ... [more]
A0A7Y4S5U6_9PROT2.600e-8979.19Crossover junction endodeoxyribonuclease RuvC n=3 ... [more]
A0A1F4AHP4_9PROT8.430e-8775.43Crossover junction endodeoxyribonuclease RuvC n=1 ... [more]
A0A1G0BXM2_9PROT1.420e-8677.19Crossover junction endodeoxyribonuclease RuvC n=3 ... [more]
A0A522X4L9_9PROT2.470e-8676.30Crossover junction endodeoxyribonuclease RuvC n=2 ... [more]
A0A1F3WY67_9PROT5.760e-8675.58Crossover junction endodeoxyribonuclease RuvC n=2 ... [more]
D9SCK9_GALCS3.940e-8576.30Crossover junction endodeoxyribonuclease RuvC n=11... [more]
A0A2Z6GBN2_9PROT4.620e-8577.19Crossover junction endodeoxyribonuclease RuvC n=9 ... [more]
D5CMR6_SIDLE6.270e-8575.29Crossover junction endodeoxyribonuclease RuvC n=8 ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig17994contigP-wetherbeei_contig17994:513..1040 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start0
Seed ortholog395494.Galf_2593
Preferred nameruvC
PFAMsRuvC
Model size528
Max annot lvl28216|Betaproteobacteria
KEGG koko:K01159
KEGG Pathwayko03440,map03440
Hectar predicted targeting categoryother localisation
Exons1
Evalue6.46e-86
EggNOG OGsCOG0817@1|root,COG0817@2|Bacteria,1MUJI@1224|Proteobacteria,2VQ3U@28216|Betaproteobacteria,44VRQ@713636|Nitrosomonadales
EC3.1.22.4
DescriptionNuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group
Cds size528
COG categoryL
BRITEko00000,ko00001,ko01000,ko03400
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683186132.9696903-CDS-P-wetherbeei_contig17994:512..10401683186132.9696903-CDS-P-wetherbeei_contig17994:512..1040Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig17994 513..1040 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig17994.2.1prot_P-wetherbeei_contig17994.2.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig17994 513..1040 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig17994.2.1

>prot_P-wetherbeei_contig17994.2.1 ID=prot_P-wetherbeei_contig17994.2.1|Name=mRNA_P-wetherbeei_contig17994.2.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=176bp
AESIRILGIDPGLRVTGFGVIDKVGTRLTYVTSGCVKSGEGELPERIKVL
LEGLSEVIAANRPDQSAIEKVFVNVNPQSTLLLGQARGAAICAAVLAGLP
VAEYTALQVKQSVVGAGKAAKEQVQMMVKRLLSLAGDPSPDAADALACAI
CHAHAGQGLGAFPTKGRRMKDGRLV*
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mRNA from alignment at P-wetherbeei_contig17994:513..1040-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig17994.2.1 ID=mRNA_P-wetherbeei_contig17994.2.1|Name=mRNA_P-wetherbeei_contig17994.2.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=528bp|location=Sequence derived from alignment at P-wetherbeei_contig17994:513..1040- (Phaeothamnion wetherbeei SAG_119_79)
GCCGAGTCCATCCGCATCCTCGGCATCGACCCTGGCCTGAGGGTCACCGG
GTTCGGCGTGATCGACAAGGTCGGCACCCGCCTCACCTACGTCACCAGCG
GCTGCGTCAAGAGCGGCGAGGGCGAGCTGCCCGAGCGCATCAAGGTGCTG
CTCGAGGGCCTGTCCGAGGTGATTGCCGCCAACCGGCCAGACCAGTCCGC
GATAGAGAAAGTCTTCGTCAACGTGAACCCGCAGTCCACGCTGCTGCTCG
GCCAGGCGCGCGGCGCGGCGATCTGCGCGGCGGTCCTCGCGGGCCTGCCG
GTCGCCGAGTACACTGCCCTGCAGGTCAAGCAATCCGTCGTAGGGGCGGG
CAAGGCCGCCAAGGAGCAGGTGCAGATGATGGTGAAGCGGTTGCTTTCGC
TCGCGGGCGATCCCAGCCCGGACGCGGCCGACGCGCTGGCCTGCGCGATC
TGCCACGCGCATGCCGGCCAGGGCCTGGGCGCGTTCCCCACCAAGGGTCG
CCGCATGAAAGACGGGAGGCTGGTGTGA
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Coding sequence (CDS) from alignment at P-wetherbeei_contig17994:513..1040-

>mRNA_P-wetherbeei_contig17994.2.1 ID=mRNA_P-wetherbeei_contig17994.2.1|Name=mRNA_P-wetherbeei_contig17994.2.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=528bp|location=Sequence derived from alignment at P-wetherbeei_contig17994:513..1040- (Phaeothamnion wetherbeei SAG_119_79)
GCCGAGTCCATCCGCATCCTCGGCATCGACCCTGGCCTGAGGGTCACCGG
GTTCGGCGTGATCGACAAGGTCGGCACCCGCCTCACCTACGTCACCAGCG
GCTGCGTCAAGAGCGGCGAGGGCGAGCTGCCCGAGCGCATCAAGGTGCTG
CTCGAGGGCCTGTCCGAGGTGATTGCCGCCAACCGGCCAGACCAGTCCGC
GATAGAGAAAGTCTTCGTCAACGTGAACCCGCAGTCCACGCTGCTGCTCG
GCCAGGCGCGCGGCGCGGCGATCTGCGCGGCGGTCCTCGCGGGCCTGCCG
GTCGCCGAGTACACTGCCCTGCAGGTCAAGCAATCCGTCGTAGGGGCGGG
CAAGGCCGCCAAGGAGCAGGTGCAGATGATGGTGAAGCGGTTGCTTTCGC
TCGCGGGCGATCCCAGCCCGGACGCGGCCGACGCGCTGGCCTGCGCGATC
TGCCACGCGCATGCCGGCCAGGGCCTGGGCGCGTTCCCCACCAAGGGTCG
CCGCATGAAAGACGGGAGGCTGGTGTGA
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