mRNA_P-wetherbeei_contig1045.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig1045.1.1
Unique NamemRNA_P-wetherbeei_contig1045.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig1045.1.1 vs. uniprot
Match: D8LDX7_ECTSI (Serine/threonine-protein phosphatase n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LDX7_ECTSI)

HSP 1 Score: 73.6 bits (179), Expect = 4.490e-10
Identity = 64/232 (27.59%), Postives = 88/232 (37.93%), Query Frame = 1 
Query:  568 GDKLGFEVWEQIQDIFDRLPLAAVVDHDIFCSYSGIPPEAPGTAAANLTSPVSSRTPSLRRXXXXXXXXXXGTAGGIGAAIDGAAAXXXXXXXXXXXXXXXXXXXXXAELNALQEIMSLPPVLPLLPPGADVSARQLQVAQSLLWGVPLTEPQEKELLQGSSHGSAVAVASVXXXXXXDASGMPAPATFGRKAVADFLSRHNLSFVCRGGLAADGTGPHGVALSQAAKVFTV 1263
            G+ LG +VWE    +FDRLPLA V+D DIFC + GIP             P+   T                                                      + +Q+IM +PPV  + PP    +    QVA   +W  P  + QE  L + S  G ++                     FG KA+ DFL++ NLS++ R    A      GV+LS+ AKVFTV
Sbjct:  246 GEALGEKVWESCNAVFDRLPLAGVIDQDIFCVHGGIP------------RPLEGST------------------------------------------------------SRIQDIMCVPPVASVCPPNECETFESQQVASDCMWSDPAKDEQELAL-EDSGFGESLRGGGAIC--------------FGTKAIDDFLTQGNLSYIVR----AHEAHSEGVSLSKGAKVFTV 392
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BLAST of mRNA_P-wetherbeei_contig1045.1.1 vs. uniprot
Match: A0A7S2ULL1_9STRA (Serine/threonine-protein phosphatase (Fragment) n=1 Tax=Attheya septentrionalis TaxID=420275 RepID=A0A7S2ULL1_9STRA)

HSP 1 Score: 62.0 bits (149), Expect = 1.640e-7
Identity = 26/37 (70.27%), Postives = 29/37 (78.38%), Query Frame = 1 
Query:  568 GDKLGFEVWEQIQDIFDRLPLAAVVDHDIFCSYSGIP 678
            GD LGF VWEQ   +FDRLPLAAV+D DIFC + GIP
Sbjct:   92 GDDLGFRVWEQTNQVFDRLPLAAVIDQDIFCVHGGIP 128
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BLAST of mRNA_P-wetherbeei_contig1045.1.1 vs. uniprot
Match: A0A7S2R9H0_9STRA (Hypothetical protein (Fragment) n=1 Tax=Eucampia antarctica TaxID=49252 RepID=A0A7S2R9H0_9STRA)

HSP 1 Score: 60.1 bits (144), Expect = 2.170e-7
Identity = 24/37 (64.86%), Postives = 29/37 (78.38%), Query Frame = 1 
Query:  568 GDKLGFEVWEQIQDIFDRLPLAAVVDHDIFCSYSGIP 678
            GD+LGF+VWEQ   +FDRLPL  V+D DIFC + GIP
Sbjct:   53 GDELGFKVWEQTNQVFDRLPLVGVIDQDIFCVHGGIP 89
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BLAST of mRNA_P-wetherbeei_contig1045.1.1 vs. uniprot
Match: A0A1D2N6G8_ORCCI (Serine/threonine-protein phosphatase n=1 Tax=Orchesella cincta TaxID=48709 RepID=A0A1D2N6G8_ORCCI)

HSP 1 Score: 63.2 bits (152), Expect = 7.610e-7
Identity = 27/64 (42.19%), Postives = 40/64 (62.50%), Query Frame = 1 
Query:  547 QQSVALLGDKLGFEVWEQIQDIFDRLPLAAVVDHDIFCSYSGIPPEAPGTAAANLTSPVSSRTP 738
            ++ +   GDKLG E+W  + D+FD +PLAAV+D  IFC++ GIPP   G+ A  +   + S  P
Sbjct:  254 KECLTKFGDKLGREIWNAVNDVFDVMPLAAVIDKKIFCAHGGIPPPWIGSGAVEIIKSIPSPLP 317
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BLAST of mRNA_P-wetherbeei_contig1045.1.1 vs. uniprot
Match: A0A7S2WM62_9STRA (Serine/threonine-protein phosphatase n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2WM62_9STRA)

HSP 1 Score: 63.5 bits (153), Expect = 7.940e-7
Identity = 29/67 (43.28%), Postives = 41/67 (61.19%), Query Frame = 1 
Query:  544 LQQSVALLGDKLGFEVWEQIQDIFDRLPLAAVVDHDIFCSYSGIPPEAPGTAAANLTSPVSSRTPSL 744
            + Q  +  G+  G EVWE++  +FDRLPLAAV+DH+IFC + GIP   PG+A       +  R P +
Sbjct:  325 IAQCKSRFGEDRGEEVWEELNQVFDRLPLAAVIDHEIFCIHGGIPRPLPGSAPGASRLDMICRVPPV 391
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BLAST of mRNA_P-wetherbeei_contig1045.1.1 vs. uniprot
Match: A0A6V1NP81_HETAK (Serine/threonine-protein phosphatase n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6V1NP81_HETAK)

HSP 1 Score: 63.2 bits (152), Expect = 8.350e-7
Identity = 23/43 (53.49%), Postives = 34/43 (79.07%), Query Frame = 1 
Query:  568 GDKLGFEVWEQIQDIFDRLPLAAVVDHDIFCSYSGIPPEAPGT 696
            G+++G  VWE++  +FDRLPLAAV+DHD+FC + G+P   PG+
Sbjct:  236 GEEMGIRVWEEVNQVFDRLPLAAVIDHDVFCVHGGLPRPIPGS 278
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BLAST of mRNA_P-wetherbeei_contig1045.1.1 vs. uniprot
Match: A0A7S0YR00_9CRYP (Serine/threonine-protein phosphatase (Fragment) n=1 Tax=Hemiselmis tepida TaxID=464990 RepID=A0A7S0YR00_9CRYP)

HSP 1 Score: 62.8 bits (151), Expect = 1.050e-6
Identity = 32/75 (42.67%), Postives = 46/75 (61.33%), Query Frame = 1 
Query:  523 AGAAPPLLQQSVALLGDKLGFEVWEQIQDIFDRLPLAAVVDHDIFCSYSGIPPEAPGTAAANLTSPVSSRTPSLR 747
            AG AP L Q   +L G+++G  VWE I D+FD LPLAA++D  +FC++ GIP         +L SPV ++   +R
Sbjct:  165 AGYAPCLQQCCTSLFGEEVGMSVWESINDVFDVLPLAAIIDRSVFCTHGGIPDP-------SLLSPVKTQDQLVR 232
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BLAST of mRNA_P-wetherbeei_contig1045.1.1 vs. uniprot
Match: A0A7R9VN74_9STRA (Serine/threonine-protein phosphatase (Fragment) n=1 Tax=Pseudictyota dubia TaxID=2749911 RepID=A0A7R9VN74_9STRA)

HSP 1 Score: 62.0 bits (149), Expect = 1.370e-6
Identity = 26/37 (70.27%), Postives = 29/37 (78.38%), Query Frame = 1 
Query:  568 GDKLGFEVWEQIQDIFDRLPLAAVVDHDIFCSYSGIP 678
            GD LGF VWEQ   +FDRLPLAAV+D DIFC + GIP
Sbjct:  259 GDDLGFRVWEQTNQVFDRLPLAAVIDQDIFCVHGGIP 295
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BLAST of mRNA_P-wetherbeei_contig1045.1.1 vs. uniprot
Match: A0A7S1GWJ1_HEMAN (Serine/threonine-protein phosphatase (Fragment) n=2 Tax=Hemiselmis andersenii TaxID=464988 RepID=A0A7S1GWJ1_HEMAN)

HSP 1 Score: 62.0 bits (149), Expect = 1.650e-6
Identity = 26/52 (50.00%), Postives = 38/52 (73.08%), Query Frame = 1 
Query:  523 AGAAPPLLQQSVALLGDKLGFEVWEQIQDIFDRLPLAAVVDHDIFCSYSGIP 678
            AG +P L Q  ++L G+++G  VWE I D+FD LPLAA++D  +FC++ GIP
Sbjct:  179 AGYSPCLQQCCISLFGEEVGMSVWESINDVFDVLPLAAIIDRSVFCTHGGIP 230
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BLAST of mRNA_P-wetherbeei_contig1045.1.1 vs. uniprot
Match: A0A7S4IG93_9STRA (Serine/threonine-protein phosphatase n=2 Tax=Odontella aurita TaxID=265563 RepID=A0A7S4IG93_9STRA)

HSP 1 Score: 62.0 bits (149), Expect = 2.550e-6
Identity = 26/37 (70.27%), Postives = 29/37 (78.38%), Query Frame = 1 
Query:  568 GDKLGFEVWEQIQDIFDRLPLAAVVDHDIFCSYSGIP 678
            GD LGF VWEQ   +FDRLPLAAV+D DIFC + GIP
Sbjct:  286 GDDLGFRVWEQTNQVFDRLPLAAVIDQDIFCVHGGIP 322
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig1045.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 24
Match NameE-valueIdentityDescription
D8LDX7_ECTSI4.490e-1027.59Serine/threonine-protein phosphatase n=2 Tax=Ectoc... [more]
A0A7S2ULL1_9STRA1.640e-770.27Serine/threonine-protein phosphatase (Fragment) n=... [more]
A0A7S2R9H0_9STRA2.170e-764.86Hypothetical protein (Fragment) n=1 Tax=Eucampia a... [more]
A0A1D2N6G8_ORCCI7.610e-742.19Serine/threonine-protein phosphatase n=1 Tax=Orche... [more]
A0A7S2WM62_9STRA7.940e-743.28Serine/threonine-protein phosphatase n=1 Tax=Rhizo... [more]
A0A6V1NP81_HETAK8.350e-753.49Serine/threonine-protein phosphatase n=1 Tax=Heter... [more]
A0A7S0YR00_9CRYP1.050e-642.67Serine/threonine-protein phosphatase (Fragment) n=... [more]
A0A7R9VN74_9STRA1.370e-670.27Serine/threonine-protein phosphatase (Fragment) n=... [more]
A0A7S1GWJ1_HEMAN1.650e-650.00Serine/threonine-protein phosphatase (Fragment) n=... [more]
A0A7S4IG93_9STRA2.550e-670.27Serine/threonine-protein phosphatase n=2 Tax=Odont... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig1045contigP-wetherbeei_contig1045:5714..7719 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog2880.D8LDX7
PFAMsMetallophos,Plus-3
Model size1838
Max annot lvl2759|Eukaryota
KEGG koko:K01090
Hectar predicted targeting categoryother localisation
Exons2
Evalue7.35e-11
EggNOG OGsCOG0639@1|root,KOG0374@2759|Eukaryota
EC3.1.3.16
Descriptionphosphoprotein phosphatase activity
Cds size1836
COG categoryT
BRITEko00000,ko01000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683185869.8566012-CDS-P-wetherbeei_contig1045:5713..63131683185869.8566012-CDS-P-wetherbeei_contig1045:5713..6313Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig1045 5714..6313 +
1683185869.8662055-CDS-P-wetherbeei_contig1045:6481..77171683185869.8662055-CDS-P-wetherbeei_contig1045:6481..7717Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig1045 6482..7717 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig1045.1.1prot_P-wetherbeei_contig1045.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig1045 5714..7717 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig1045.1.1

>prot_P-wetherbeei_contig1045.1.1 ID=prot_P-wetherbeei_contig1045.1.1|Name=mRNA_P-wetherbeei_contig1045.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=612bp
LNGSSGCVRRKGSSRLGRTGRRGSGSSSGSGSRKSSLTAGARLIGSLGGS
FGGSGASSIGGFTPAYGGTPLEFDADAVAAAALTLPEGGADTSFGACNSA
GTAMEVDPPVPLKAAPVAPAAPAAPAVGLNVSDLLPPSPERTRNAPLAIV
PGVAAAGAAVSMPVQGPSVMAAAAAGAAPPLLQQSVALLGDKLGFEVWEQ
IQDIFDRLPLAAVVDHDIFCSYSGIPPEAPGTAAANLTSPVSSRTPSLRR
RRTTSAAKPPGTAGGIGAAIDGAAAVAAAAAALASGAPGIAAAAAAAELN
ALQEIMSLPPVLPLLPPGADVSARQLQVAQSLLWGVPLTEPQEKELLQGS
SHGSAVAVASVAAAAAADASGMPAPATFGRKAVADFLSRHNLSFVCRGGL
AADGTGPHGVALSQAAKVFTVACSPPRRSRAATLAAGADAAAAAAAAEAV
AGAAVINPGGGRSSLRPLSLGRSSARNAAAAGGGSSGAGGNSSRSPGGGR
SRPWSMTRKRGNAAEDATFADAEGISGFGACGCLLIDFDKIVAVGRAPNY
QRIHHKSGSGATGSITVAGIGTVPELEGWSGSRKGSGGNAGVKTMTAAEA
VGAAALGGRAAE
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mRNA from alignment at P-wetherbeei_contig1045:5714..7719+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig1045.1.1 ID=mRNA_P-wetherbeei_contig1045.1.1|Name=mRNA_P-wetherbeei_contig1045.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=2006bp|location=Sequence derived from alignment at P-wetherbeei_contig1045:5714..7719+ (Phaeothamnion wetherbeei SAG_119_79)
CTCAACGGAAGCTCTGGCTGCGTCCGCCGCAAAGGCAGCAGCAGGCTCGG
CCGCACCGGCCGCCGTGGCAGCGGCAGCAGCAGCGGAAGCGGCAGTAGAA
AATCCAGTTTGACTGCCGGCGCTCGCCTCATTGGCAGTCTTGGCGGCAGC
TTCGGCGGCAGCGGCGCTTCCAGCATCGGCGGTTTCACCCCGGCATATGG
TGGCACTCCTTTGGAATTCGACGCCGACGCCGTTGCTGCCGCCGCTCTCA
CGCTACCCGAGGGAGGAGCGGACACTTCCTTCGGCGCTTGCAATTCGGCG
GGGACCGCCATGGAGGTGGACCCGCCGGTTCCGCTGAAAGCGGCACCGGT
GGCGCCTGCTGCACCCGCCGCGCCAGCTGTCGGCCTGAACGTATCCGATC
TGTTGCCGCCGTCGCCGGAGCGGACACGCAATGCCCCGCTGGCGATAGTG
CCGGGAGTTGCGGCGGCGGGGGCGGCGGTGTCGATGCCGGTGCAGGGGCC
TTCGGTAATGGCGGCGGCGGCAGCAGGAGCTGCTCCGCCGCTGCTGCAGC
AAAGCGTGGCGCTTTTGGGGGACAAGCTCGGCTTTGAAGTCTGGGAGCAG
GTGGGTTATGCGAAGGGTGATGGACGACCGCCCGAAGTGACTCGCAAAAG
AACTGCGATTATTCTTGTGGGCCTGTGATATTCACAAGAGGAGCTTTGCA
GGGGGCTTATCCTTTTGTCACTTCTCCGCTATTACAATATTTGGTTTTCA
CTGCCGTCCTATCCGCAGATCCAGGACATTTTTGACAGGCTGCCGCTGGC
GGCGGTCGTGGACCACGACATCTTTTGCTCGTACAGCGGCATCCCGCCGG
AGGCACCGGGAACGGCCGCCGCAAATTTGACTTCACCAGTTTCGTCGCGG
ACGCCCTCGCTGCGGCGGCGCCGCACGACGTCGGCGGCGAAGCCGCCGGG
CACCGCGGGCGGCATCGGAGCCGCCATCGATGGAGCGGCGGCAGTGGCGG
CGGCGGCCGCCGCTTTGGCATCGGGTGCACCGGGCATCGCTGCGGCGGCG
GCGGCGGCAGAGTTGAATGCGCTCCAGGAGATTATGTCACTGCCGCCAGT
GCTGCCGTTGCTGCCGCCGGGCGCCGATGTCTCGGCGCGGCAGCTGCAGG
TGGCGCAGAGCCTTCTCTGGGGCGTGCCGCTGACAGAGCCGCAGGAGAAA
GAGCTGCTGCAGGGCAGCAGTCACGGCAGCGCCGTGGCGGTTGCGTCAGT
GGCGGCGGCAGCCGCCGCTGACGCCTCGGGGATGCCGGCGCCAGCCACCT
TTGGGCGCAAGGCGGTGGCCGACTTCCTGTCGCGGCACAATCTGTCGTTC
GTCTGCCGCGGCGGGCTCGCGGCGGACGGAACCGGCCCACACGGCGTGGC
GCTGTCCCAGGCCGCCAAGGTCTTCACCGTTGCCTGCTCGCCGCCACGGC
GGTCGCGTGCTGCCACCCTCGCCGCTGGCGCCGACGCGGCGGCCGCAGCG
GCGGCGGCGGAGGCGGTTGCAGGAGCTGCTGTCATCAATCCGGGTGGCGG
CCGCTCGTCGCTGCGCCCGCTGAGCCTTGGTCGGTCAAGTGCTCGCAACG
CTGCCGCCGCCGGCGGAGGCAGCAGCGGGGCTGGTGGCAACAGCAGTCGC
AGTCCTGGTGGTGGCCGTTCCCGGCCGTGGAGTATGACCCGCAAGCGCGG
CAACGCGGCCGAAGACGCCACTTTCGCCGACGCGGAGGGAATCTCTGGCT
TTGGCGCCTGCGGTTGCCTGCTAATTGACTTTGACAAAATTGTCGCTGTC
GGACGGGCGCCGAACTACCAGCGCATCCACCACAAAAGCGGCAGCGGCGC
TACCGGCAGCATAACCGTCGCCGGCATCGGCACCGTCCCCGAGCTGGAAG
GCTGGTCGGGCTCCCGGAAAGGCAGTGGCGGCAACGCCGGCGTCAAGACG
ATGACGGCGGCGGAGGCGGTGGGCGCGGCAGCCCTGGGAGGACGGGCGGC
CGAGGC
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Coding sequence (CDS) from alignment at P-wetherbeei_contig1045:5714..7719+

>mRNA_P-wetherbeei_contig1045.1.1 ID=mRNA_P-wetherbeei_contig1045.1.1|Name=mRNA_P-wetherbeei_contig1045.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=1836bp|location=Sequence derived from alignment at P-wetherbeei_contig1045:5714..7719+ (Phaeothamnion wetherbeei SAG_119_79)
CTCAACGGAAGCTCTGGCTGCGTCCGCCGCAAAGGCAGCAGCAGGCTCGG
CCGCACCGGCCGCCGTGGCAGCGGCAGCAGCAGCGGAAGCGGCAGTAGAA
AATCCAGTTTGACTGCCGGCGCTCGCCTCATTGGCAGTCTTGGCGGCAGC
TTCGGCGGCAGCGGCGCTTCCAGCATCGGCGGTTTCACCCCGGCATATGG
TGGCACTCCTTTGGAATTCGACGCCGACGCCGTTGCTGCCGCCGCTCTCA
CGCTACCCGAGGGAGGAGCGGACACTTCCTTCGGCGCTTGCAATTCGGCG
GGGACCGCCATGGAGGTGGACCCGCCGGTTCCGCTGAAAGCGGCACCGGT
GGCGCCTGCTGCACCCGCCGCGCCAGCTGTCGGCCTGAACGTATCCGATC
TGTTGCCGCCGTCGCCGGAGCGGACACGCAATGCCCCGCTGGCGATAGTG
CCGGGAGTTGCGGCGGCGGGGGCGGCGGTGTCGATGCCGGTGCAGGGGCC
TTCGGTAATGGCGGCGGCGGCAGCAGGAGCTGCTCCGCCGCTGCTGCAGC
AAAGCGTGGCGCTTTTGGGGGACAAGCTCGGCTTTGAAGTCTGGGAGCAG
ATCCAGGACATTTTTGACAGGCTGCCGCTGGCGGCGGTCGTGGACCACGA
CATCTTTTGCTCGTACAGCGGCATCCCGCCGGAGGCACCGGGAACGGCCG
CCGCAAATTTGACTTCACCAGTTTCGTCGCGGACGCCCTCGCTGCGGCGG
CGCCGCACGACGTCGGCGGCGAAGCCGCCGGGCACCGCGGGCGGCATCGG
AGCCGCCATCGATGGAGCGGCGGCAGTGGCGGCGGCGGCCGCCGCTTTGG
CATCGGGTGCACCGGGCATCGCTGCGGCGGCGGCGGCGGCAGAGTTGAAT
GCGCTCCAGGAGATTATGTCACTGCCGCCAGTGCTGCCGTTGCTGCCGCC
GGGCGCCGATGTCTCGGCGCGGCAGCTGCAGGTGGCGCAGAGCCTTCTCT
GGGGCGTGCCGCTGACAGAGCCGCAGGAGAAAGAGCTGCTGCAGGGCAGC
AGTCACGGCAGCGCCGTGGCGGTTGCGTCAGTGGCGGCGGCAGCCGCCGC
TGACGCCTCGGGGATGCCGGCGCCAGCCACCTTTGGGCGCAAGGCGGTGG
CCGACTTCCTGTCGCGGCACAATCTGTCGTTCGTCTGCCGCGGCGGGCTC
GCGGCGGACGGAACCGGCCCACACGGCGTGGCGCTGTCCCAGGCCGCCAA
GGTCTTCACCGTTGCCTGCTCGCCGCCACGGCGGTCGCGTGCTGCCACCC
TCGCCGCTGGCGCCGACGCGGCGGCCGCAGCGGCGGCGGCGGAGGCGGTT
GCAGGAGCTGCTGTCATCAATCCGGGTGGCGGCCGCTCGTCGCTGCGCCC
GCTGAGCCTTGGTCGGTCAAGTGCTCGCAACGCTGCCGCCGCCGGCGGAG
GCAGCAGCGGGGCTGGTGGCAACAGCAGTCGCAGTCCTGGTGGTGGCCGT
TCCCGGCCGTGGAGTATGACCCGCAAGCGCGGCAACGCGGCCGAAGACGC
CACTTTCGCCGACGCGGAGGGAATCTCTGGCTTTGGCGCCTGCGGTTGCC
TGCTAATTGACTTTGACAAAATTGTCGCTGTCGGACGGGCGCCGAACTAC
CAGCGCATCCACCACAAAAGCGGCAGCGGCGCTACCGGCAGCATAACCGT
CGCCGGCATCGGCACCGTCCCCGAGCTGGAAGGCTGGTCGGGCTCCCGGA
AAGGCAGTGGCGGCAACGCCGGCGTCAAGACGATGACGGCGGCGGAGGCG
GTGGGCGCGGCAGCCCTGGGAGGACGGGCGGCCGAG
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