mRNA_M-pyrifera_M_contig89332.20708.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig89332.20708.1
Unique NamemRNA_M-pyrifera_M_contig89332.20708.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig89332.20708.1 vs. uniprot
Match: A0A5A8CR06_CAFRO (Uncharacterized protein n=3 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8CR06_CAFRO)

HSP 1 Score: 124 bits (310), Expect = 3.910e-29
Identity = 72/198 (36.36%), Postives = 113/198 (57.07%), Query Frame = 1
Query:    4 REQVVQSFLVIAFLLHTPISKAAFRIMTCRPVGPTDSSGLDDPRTQLRLSMDMNYICTGN-TLGRMVGMGLPVVLCFSFGLPIAAWIFLRQQGGSRRVGDPLLRSRLGFYYNGYSPAYFYWESIVLLRKVTLAMVTVLLGPYGGGIQALTALLLILFSLVLQSWAKPYEEGSLNKLELFALVVAAVTLVGGLYVVFDN 594
            R++++ S  V+ F++HT ++KAA  + TCR +   D +G ++      L+ DM   C    T+G  +  GLP  L FS G+PIAA++ LR++    ++  P +R   GF Y G+    ++WES+V+ RKV L+++ V L PYG  +QA  AL ++  + +L     PYEE S N LEL +LV   +T+  GL     N
Sbjct: 3152 RDRLIVSVFVVLFMIHTTVTKAALSLFTCRWI--RDETGREEATEWQVLTADMTVGCNDPATVGWKLAAGLPTFLLFSCGVPIAAYLALRRE--RHKLERPRVRQTYGFLYAGFRSHVYFWESVVMARKVLLSVIVVFLEPYGVDVQAYVALAVLFAAALLHVATWPYEETSANSLELLSLVACFLTITLGLMTFSPN 3345          
BLAST of mRNA_M-pyrifera_M_contig89332.20708.1 vs. uniprot
Match: A0A067C6E6_SAPPC (Uncharacterized protein n=1 Tax=Saprolegnia parasitica (strain CBS 223.65) TaxID=695850 RepID=A0A067C6E6_SAPPC)

HSP 1 Score: 107 bits (266), Expect = 2.810e-23
Identity = 67/192 (34.90%), Postives = 109/192 (56.77%), Query Frame = 1
Query:    7 EQVVQSFLVIAFLLHTPISKAAFRIMTCRPVGPTDSSGLDDPRTQLRLSMDMNYIC-TGNTLGRMVGMGLPVVLCFSFGLPIAAWIFLRQQGGSRRVGDPLLRSRLGFYYNGYSPAYFYWESIVLLRKVTLAMVTVLLGPYGGGIQALTALLLILFSLVLQSWAKPYEEGSLNKLELFALVVAAVTLVGGLY 579
            ++ V S +V+ FL+H  ++K  F++ +C  +G  D  G+        L  D++  C   +    M+G+GLP VLC +FG+P  A+  LR +  +  + DP  + + GF Y+GY  A++YWE  +++RKV ++MV+V L  +G G Q+L A  LI  +L +   A PYE   +N LE  +L+    T+  GLY
Sbjct:  981 DKSVLSIIVLMFLIHPGVTKQIFQLFSCSELG-MDEHGV----AMAFLDADLDIPCYDASHFKWMLGVGLPSVLCITFGIPGFAYGVLRARRAT--LDDPRTQLQFGFLYDGYKLAHYYWEIWIMMRKVLVSMVSVFLKSWGTGPQSLGATGLIFVALYIHMEATPYELDVVNNLEQTSLLTCLFTMYFGLY 1165          
BLAST of mRNA_M-pyrifera_M_contig89332.20708.1 vs. uniprot
Match: J9I2F5_9SPIT (Uncharacterized protein n=1 Tax=Oxytricha trifallax TaxID=1172189 RepID=J9I2F5_9SPIT)

HSP 1 Score: 97.8 bits (242), Expect = 4.850e-20
Identity = 64/207 (30.92%), Postives = 112/207 (54.11%), Query Frame = 1
Query:    4 REQVVQSFLVIAFLLHTPISKAAFRIMTCRPVGPTDSSGLDDPRTQLRLSMDMNYICTGNTLGRMVGMGLPVVLCFSFGLPIAAWIFL-RQQGGSRRVG--DPL----------LRSRLGFYYNGYSPAYFYWESIVLLRKVTLAMVTVLLGPYGGGIQALTALLLILFSLVLQSWAKPYEEGSLNKLELFALVVAAVTLVGGLYVV 585
            R++++ + +++ FL+H  I+K  F+   C  V         D   +L+ ++DM      + +   V + +P ++ +  G+P+A+ + L R +    RVG  D L          L+ + GF++NGY    F+WE I+L RK+++ MV+V         Q L  +L+I+ SL+LQ   KP+    LNK+ELF+L VA +T+ GG++ V
Sbjct: 2346 RDKLISTMVILLFLVHPTIAKILFQAFNCIEV---------DGINRLKSNIDMVCYKDQHMIYLFVVV-IPGIIIWVIGIPLASLVMLLRNREKIMRVGKYDELSQSDKYQIIHLKMKYGFFFNGYKLGTFFWEVIILYRKISIVMVSVFFSVVSAEAQVLVVILIIVVSLLLQIKYKPFYTDELNKMELFSLQVAVITMYGGMFYV 2542          
BLAST of mRNA_M-pyrifera_M_contig89332.20708.1 vs. uniprot
Match: A0A835T2T5_9CHLO (EF-hand domain-containing protein n=1 Tax=Chlamydomonas schloesseri TaxID=2026947 RepID=A0A835T2T5_9CHLO)

HSP 1 Score: 97.4 bits (241), Expect = 6.660e-20
Identity = 49/132 (37.12%), Postives = 80/132 (60.61%), Query Frame = 1
Query:  199 VGMGLPVVLCFSFGLPIAAWIFLRQQGGSRRVGDPLLRSRLGFYYNGYSPAYFYWESIVLLRKVTLAMVTVLLGPYGGGIQALTALLLILFSLVLQSWAKPYEEGSLNKLELFALVVAAVTLVGGLYVVFDN 594
            +G+ LP+V+ FS G+P+A+ +FLR    SR   DP   ++ GF Y  Y P YFYWESI++ RK+ + +V V +G  G G+Q L A+ +++ + V+Q    P+ E ++N LE  +L   + TL   L++  + 
Sbjct: 3873 MGLALPLVVVFSLGVPVASGVFLRYNR-SRLENDPAFAAKYGFVYGEYRPRYFYWESIIMFRKLIMVVVIVYVGYVGVGVQVLVAMGVLMLATVVQLLCSPFREPAMNDLERLSLYSTSATLYLSLFLYVEG 4003          
BLAST of mRNA_M-pyrifera_M_contig89332.20708.1 vs. uniprot
Match: A0A8J4BME7_9CHLO (TRP domain-containing protein n=3 Tax=Volvox africanus TaxID=51714 RepID=A0A8J4BME7_9CHLO)

HSP 1 Score: 94.4 bits (233), Expect = 7.570e-19
Identity = 64/203 (31.53%), Postives = 104/203 (51.23%), Query Frame = 1
Query:    4 REQVVQSFLVIAFLLHTPISKAAFRIMTCRPVGPTDSSGLDDPRTQLR------LSMDMNYICTGNT-LGRMVGMGLPVVLCFSFGLPIAAWIFLRQQGGSRRVGDPLLRSRLGFYYNGYSPAYFYWESIVLLRKVTLAMVTVLLGPYGGGIQALTALLLILFSLVLQSWAKPYEEGSLNKLELFALVVAAVTLVGGLYVVFD 591
            R +++ + + I F ++  ++ A   + +C+ V P     L  P  QL        S D++  C     L   + +GLP V+ FS G P+ +W +LRQ        +   R   GF Y  YS  ++YW+SIV+LRK+ + +V V LG     +Q LTAL +I+ +++ Q  A+PY+   +N LE  +L+   V L   L+ V D
Sbjct: 1758 RPRLIITTIAIVFYVYPDVTNAFLSLFSCQLVDP-----LTGPYVQLMEARGYFWSSDLDLECYRQPHLWLALAVGLPGVVLFSAGCPVFSWFWLRQHYDVLFKSEEF-RVSYGFMYEDYSRMHYYWDSIVMLRKLAIVVVIVFLGSASPSLQVLTALGIIMSAMIAQISARPYKHQRMNDLERLSLLATMVMLYCALFFVSD 1954          
BLAST of mRNA_M-pyrifera_M_contig89332.20708.1 vs. uniprot
Match: A0A835T3F6_CHLIN (TRP domain-containing protein n=1 Tax=Chlamydomonas incerta TaxID=51695 RepID=A0A835T3F6_CHLIN)

HSP 1 Score: 92.4 bits (228), Expect = 3.600e-18
Identity = 59/198 (29.80%), Postives = 98/198 (49.49%), Query Frame = 1
Query:   10 QVVQSFLVIAFLLHTPISKAAFRIMTCRPVGPTDSS---GLDDPRTQLRLSMDMNYICTGNT-LGRMVGMGLPVVLCFSFGLPIAAWIFLRQQGGSRRVGDPLLRSRLGFYYNGYSPAYFYWESIVLLRKVTLAMVTVLLGPYGGGIQALTALLLILFSLVLQSWAKPYEEGSLNKLELFALVVAAVTLVGGLYVVFD 591
            +++ + + I F ++  I+ A   + +C  V P  +    G    +  L  + D+N  C  N  L   +G+GLP ++  S G P+ +W++LR+    +    P      GF Y  Y+   +YW+SI++ RK+ + M  V L PY   +Q LT L +I+ SLV Q    PY+   +N+LE  +L      L   LY + D
Sbjct: 3125 RIIITIIAIVFYVYPDITGAFMELFSCTTVDPEGADVAYGALMEQRGLYWTSDLNLACYQNPHLWLSLGVGLPGLVLISIGAPVFSWVWLRRHQ-HKLYTSPSFGVSYGFMYEDYNRNCYYWDSIIMARKLFVVMAIVFLAPYSPVLQILTTLGIIIVSLVAQISFNPYKNHRMNRLERLSLYATLAMLYCALYFLSD 3321          
BLAST of mRNA_M-pyrifera_M_contig89332.20708.1 vs. uniprot
Match: A0A7S3XLM1_HETAK (Hypothetical protein (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XLM1_HETAK)

HSP 1 Score: 91.3 bits (225), Expect = 4.820e-18
Identity = 63/200 (31.50%), Postives = 104/200 (52.00%), Query Frame = 1
Query:    1 YREQVVQSFLVIAFLLHTPISKAAFRIMTCRPVGPTDSSGLDDPRTQLRLSMDMNYIC-TGNTLGRMVGMGLPVVLCFSFGLPIAAWIFLRQQGGSRRVGDPLLRSRLGFYYNGYSPAYFYWESIVLLRKVTLAMVTVLLGPYGGGIQALTALLLILFSLVLQSWAKPYEEGSLNKLELFALVVAAVTLVGGLYVVFDNL 597
            YR Q + + +V+ F+++  +      +  C P+  TD+         L L +D+++ C T   +  +V +GLP +  ++ G+P  A   LR+     + G+  +RSR  F Y+GY    ++WE +VLLRKV    + V     G    A+T +++ L   VLQ  A+P+ + SLN LE  +L  A  T + GLY + DNL
Sbjct:  169 YRGQAILTIVVLLFIVYPTVVTETLTLFKCVPLD-TDA---------LYLEIDLDFQCFTDKHILWIVFLGLPALFFYAVGIPAIAMNTLRRHRKELQNGN--IRSRYNFLYDGYREQCYWWEGVVLLRKVAFIALGVSASQIGTTAVAVTGMIVALLVQVLQRHAQPFVDPSLNALEAASLRAALSTSICGLYFLQDNL 356          
BLAST of mRNA_M-pyrifera_M_contig89332.20708.1 vs. uniprot
Match: A8IRB8_CHLRE (Predicted protein (Fragment) n=1 Tax=Chlamydomonas reinhardtii TaxID=3055 RepID=A8IRB8_CHLRE)

HSP 1 Score: 90.9 bits (224), Expect = 1.090e-17
Identity = 58/198 (29.29%), Postives = 98/198 (49.49%), Query Frame = 1
Query:   10 QVVQSFLVIAFLLHTPISKAAFRIMTCRPVGPTDSS---GLDDPRTQLRLSMDMNYICTGNT-LGRMVGMGLPVVLCFSFGLPIAAWIFLRQQGGSRRVGDPLLRSRLGFYYNGYSPAYFYWESIVLLRKVTLAMVTVLLGPYGGGIQALTALLLILFSLVLQSWAKPYEEGSLNKLELFALVVAAVTLVGGLYVVFD 591
            +++ + + I F ++  I+ A   + +C  V P  +    G    +  L  + D++  C  N  L   +G+GLP ++  S G P+ +W++LR+    +    P      GF Y  Y+   +YW+S+V+ RK+ + M  V L PY   +Q LT L +I+ SLV Q    PY+   +N+LE  +L      L   LY + D
Sbjct:   63 RIIITIIAIVFYVYPDITGAFMELFSCTTVDPEGADVAYGALMEQRGLYWTSDLDLACYHNPHLWLALGVGLPGLILISIGAPVFSWVWLRRHQ-HKLYTSPSFGVSYGFLYEDYNRNCYYWDSVVMARKLFVVMAIVFLAPYSPVLQILTTLGIIIVSLVAQISFNPYKNQRMNRLERLSLYATLAMLYCALYFLSD 259          
BLAST of mRNA_M-pyrifera_M_contig89332.20708.1 vs. uniprot
Match: J9IN29_9SPIT (Uncharacterized protein n=1 Tax=Oxytricha trifallax TaxID=1172189 RepID=J9IN29_9SPIT)

HSP 1 Score: 90.9 bits (224), Expect = 1.220e-17
Identity = 59/209 (28.23%), Postives = 108/209 (51.67%), Query Frame = 1
Query:    1 YREQVVQSFLVIAFLLHTPISKAAFRIMTCRPVGPTDSSGLDDPRTQLRLSMDMNYIC-TGNTLGRMVGMGLPVVLCFSFGLPIAAWIFL-------------RQQGGSRRVGDPLLRSRLGFYYNGYSPAYFYWESIVLLRKVTLAMVTVLLGPYGGGIQALTALLLILFSLVLQSWAKPYEEGSLNKLELFALVVAAVTLVGGLYVV 585
            ++++++ + +V+ FL+H  I+KA F    C  +      G+       RL  ++   C  G  L  ++ + +P ++ + FG+P  A I +              +     +V   ++R+R+GF  NG+    F+WE ++L RK+ + MVTVLL       Q L  L++++ S++L     PY    LNK+EL++L VA +T+ GG++ V
Sbjct: 2547 FKDKLIGTIVVLLFLIHPSIAKALFSAFNCIEI-----EGVS------RLKDNVGMECYQGKHLVYVLVIIIPSLIIWIFGIPFVALIMMFKNKTTIIHMSKSNELSKYDKVKIKVIRTRIGFLLNGFQRNNFFWEILILYRKILIVMVTVLLNSVSAESQVLVVLMILVLSVLLHLNYNPYYTFVLNKMELYSLQVACITMYGGMFYV 2744          
BLAST of mRNA_M-pyrifera_M_contig89332.20708.1 vs. uniprot
Match: A0A2K3CPA6_CHLRE (TRP domain-containing protein n=2 Tax=Chlamydomonas reinhardtii TaxID=3055 RepID=A0A2K3CPA6_CHLRE)

HSP 1 Score: 90.9 bits (224), Expect = 1.230e-17
Identity = 58/198 (29.29%), Postives = 98/198 (49.49%), Query Frame = 1
Query:   10 QVVQSFLVIAFLLHTPISKAAFRIMTCRPVGPTDSS---GLDDPRTQLRLSMDMNYICTGNT-LGRMVGMGLPVVLCFSFGLPIAAWIFLRQQGGSRRVGDPLLRSRLGFYYNGYSPAYFYWESIVLLRKVTLAMVTVLLGPYGGGIQALTALLLILFSLVLQSWAKPYEEGSLNKLELFALVVAAVTLVGGLYVVFD 591
            +++ + + I F ++  I+ A   + +C  V P  +    G    +  L  + D++  C  N  L   +G+GLP ++  S G P+ +W++LR+    +    P      GF Y  Y+   +YW+S+V+ RK+ + M  V L PY   +Q LT L +I+ SLV Q    PY+   +N+LE  +L      L   LY + D
Sbjct: 3168 RIIITIIAIVFYVYPDITGAFMELFSCTTVDPEGADVAYGALMEQRGLYWTSDLDLACYHNPHLWLALGVGLPGLILISIGAPVFSWVWLRRHQ-HKLYTSPSFGVSYGFLYEDYNRNCYYWDSVVMARKLFVVMAIVFLAPYSPVLQILTTLGIIIVSLVAQISFNPYKNQRMNRLERLSLYATLAMLYCALYFLSD 3364          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig89332.20708.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A5A8CR06_CAFRO3.910e-2936.36Uncharacterized protein n=3 Tax=Cafeteria roenberg... [more]
A0A067C6E6_SAPPC2.810e-2334.90Uncharacterized protein n=1 Tax=Saprolegnia parasi... [more]
J9I2F5_9SPIT4.850e-2030.92Uncharacterized protein n=1 Tax=Oxytricha trifalla... [more]
A0A835T2T5_9CHLO6.660e-2037.12EF-hand domain-containing protein n=1 Tax=Chlamydo... [more]
A0A8J4BME7_9CHLO7.570e-1931.53TRP domain-containing protein n=3 Tax=Volvox afric... [more]
A0A835T3F6_CHLIN3.600e-1829.80TRP domain-containing protein n=1 Tax=Chlamydomona... [more]
A0A7S3XLM1_HETAK4.820e-1831.50Hypothetical protein (Fragment) n=1 Tax=Heterosigm... [more]
A8IRB8_CHLRE1.090e-1729.29Predicted protein (Fragment) n=1 Tax=Chlamydomonas... [more]
J9IN29_9SPIT1.220e-1728.23Uncharacterized protein n=1 Tax=Oxytricha trifalla... [more]
A0A2K3CPA6_CHLRE1.230e-1729.29TRP domain-containing protein n=2 Tax=Chlamydomona... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig89332contigM-pyrifera_M_contig89332:187..792 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score99.4
Seed ortholog evalue2.1e-18
Seed eggNOG ortholog112098.XP_008617830.1
Hectar predicted targeting categorysignal peptide
EggNOG free text desc.Parallel beta-helix repeats
EggNOG OGs2E21X@1,2S9AU@2759
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Exons1
Model size606
Cds size606
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816803.5831037-CDS-M-pyrifera_M_contig89332:186..7921622816803.5831037-CDS-M-pyrifera_M_contig89332:186..792Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89332 187..792 +
1692278188.0644982-CDS-M-pyrifera_M_contig89332:186..7921692278188.0644982-CDS-M-pyrifera_M_contig89332:186..792Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89332 187..792 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig89332.20708.1prot_M-pyrifera_M_contig89332.20708.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig89332 187..792 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig89332.20708.1

>prot_M-pyrifera_M_contig89332.20708.1 ID=prot_M-pyrifera_M_contig89332.20708.1|Name=mRNA_M-pyrifera_M_contig89332.20708.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=202bp
YREQVVQSFLVIAFLLHTPISKAAFRIMTCRPVGPTDSSGLDDPRTQLRL
SMDMNYICTGNTLGRMVGMGLPVVLCFSFGLPIAAWIFLRQQGGSRRVGD
PLLRSRLGFYYNGYSPAYFYWESIVLLRKVTLAMVTVLLGPYGGGIQALT
ALLLILFSLVLQSWAKPYEEGSLNKLELFALVVAAVTLVGGLYVVFDNLV
SP
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mRNA from alignment at M-pyrifera_M_contig89332:187..792+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig89332.20708.1 ID=mRNA_M-pyrifera_M_contig89332.20708.1|Name=mRNA_M-pyrifera_M_contig89332.20708.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=606bp|location=Sequence derived from alignment at M-pyrifera_M_contig89332:187..792+ (Macrocystis pyrifera P11B4 male)
TACCGAGAGCAGGTGGTACAATCTTTCCTCGTCATTGCGTTTCTCCTCCA CACACCAATTTCCAAAGCGGCATTTCGTATCATGACGTGTCGGCCAGTCG GACCAACAGATAGCAGTGGTCTGGATGATCCACGCACTCAGCTAAGACTG TCGATGGATATGAATTACATCTGCACGGGGAACACTCTTGGTCGTATGGT TGGAATGGGGCTTCCAGTTGTTCTTTGCTTCTCTTTTGGCCTTCCCATTG CAGCATGGATTTTCCTTCGCCAACAAGGTGGAAGTCGACGTGTGGGTGAT CCACTCCTACGTTCACGTCTGGGTTTCTACTACAATGGATACTCTCCCGC ATATTTTTACTGGGAATCAATTGTCTTACTTCGCAAAGTCACGCTTGCCA TGGTCACAGTGCTCCTTGGTCCCTACGGTGGTGGCATTCAAGCACTCACA GCGTTGCTCCTGATTCTCTTTTCCCTCGTTTTGCAATCATGGGCGAAACC GTACGAAGAAGGGAGTTTGAACAAGTTGGAACTCTTTGCATTGGTAGTTG CTGCAGTGACGCTCGTTGGTGGGTTGTATGTGGTGTTTGACAACCTTGTC AGCCCT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig89332:187..792+

>mRNA_M-pyrifera_M_contig89332.20708.1 ID=mRNA_M-pyrifera_M_contig89332.20708.1|Name=mRNA_M-pyrifera_M_contig89332.20708.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1212bp|location=Sequence derived from alignment at M-pyrifera_M_contig89332:187..792+ (Macrocystis pyrifera P11B4 male)
TACCGAGAGCAGGTGGTACAATCTTTCCTCGTCATTGCGTTTCTCCTCCA
CACACCAATTTCCAAAGCGGCATTTCGTATCATGACGTGTCGGCCAGTCG
GACCAACAGATAGCAGTGGTCTGGATGATCCACGCACTCAGCTAAGACTG
TCGATGGATATGAATTACATCTGCACGGGGAACACTCTTGGTCGTATGGT
TGGAATGGGGCTTCCAGTTGTTCTTTGCTTCTCTTTTGGCCTTCCCATTG
CAGCATGGATTTTCCTTCGCCAACAAGGTGGAAGTCGACGTGTGGGTGAT
CCACTCCTACGTTCACGTCTGGGTTTCTACTACAATGGATACTCTCCCGC
ATATTTTTACTGGGAATCAATTGTCTTACTTCGCAAAGTCACGCTTGCCA
TGGTCACAGTGCTCCTTGGTCCCTACGGTGGTGGCATTCAAGCACTCACA
GCGTTGCTCCTGATTCTCTTTTCCCTCGTTTTGCAATCATGGGCGAAACC
GTACGAAGAAGGGAGTTTGAACAAGTTGGAACTCTTTGCATTGGTAGTTG
CTGCAGTGACGCTCGTTGGTGGGTTGTATGTGGTGTTTGACAACCTTGTC
AGCCCTTACCGAGAGCAGGTGGTACAATCTTTCCTCGTCATTGCGTTTCT
CCTCCACACACCAATTTCCAAAGCGGCATTTCGTATCATGACGTGTCGGC
CAGTCGGACCAACAGATAGCAGTGGTCTGGATGATCCACGCACTCAGCTA
AGACTGTCGATGGATATGAATTACATCTGCACGGGGAACACTCTTGGTCG
TATGGTTGGAATGGGGCTTCCAGTTGTTCTTTGCTTCTCTTTTGGCCTTC
CCATTGCAGCATGGATTTTCCTTCGCCAACAAGGTGGAAGTCGACGTGTG
GGTGATCCACTCCTACGTTCACGTCTGGGTTTCTACTACAATGGATACTC
TCCCGCATATTTTTACTGGGAATCAATTGTCTTACTTCGCAAAGTCACGC
TTGCCATGGTCACAGTGCTCCTTGGTCCCTACGGTGGTGGCATTCAAGCA
CTCACAGCGTTGCTCCTGATTCTCTTTTCCCTCGTTTTGCAATCATGGGC
GAAACCGTACGAAGAAGGGAGTTTGAACAAGTTGGAACTCTTTGCATTGG
TAGTTGCTGCAGTGACGCTCGTTGGTGGGTTGTATGTGGTGTTTGACAAC
CTTGTCAGCCCT
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