mRNA_M-pyrifera_M_contig88027.20449.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig88027.20449.1
Unique NamemRNA_M-pyrifera_M_contig88027.20449.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig88027.20449.1 vs. uniprot
Match: A0A7W0V787_9CHLR (Glycine--tRNA ligase n=1 Tax=Chloroflexia bacterium TaxID=2448782 RepID=A0A7W0V787_9CHLR)

HSP 1 Score: 115 bits (288), Expect = 4.450e-31
Identity = 62/105 (59.05%), Postives = 76/105 (72.38%), Query Frame = 1 
Query:    7 DAKGKVTTRQVLRLHPDLAPFRAAVLPLSKKPQDLIDVASDLHQALLFNLGD-VDYDIKGAIGRRYRRHDEIGTPFCITVDHDSLQDNEVTVRFRDSSKQTRVPI 318
            D  GK  TR VLRLHP +AP++AAVLPL KK Q+L + A +L Q L+ + G  VDYD  G IG+RYRR DEIGTPFC T+D D+LQD+ VTVR RD  +Q R+ I
Sbjct:    7 DVNGKPDTRVVLRLHPRVAPYKAAVLPLMKK-QELAEPARNLFQTLVDSSGVLVDYDESGNIGKRYRRQDEIGTPFCFTIDFDTLQDSSVTVRHRDDMRQERLAI 110
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BLAST of mRNA_M-pyrifera_M_contig88027.20449.1 vs. uniprot
Match: UPI00199C2E9E (His/Gly/Thr/Pro-type tRNA ligase C-terminal domain-containing protein n=1 Tax=Salmonella enterica TaxID=28901 RepID=UPI00199C2E9E)

HSP 1 Score: 115 bits (289), Expect = 9.340e-31
Identity = 64/103 (62.14%), Postives = 73/103 (70.87%), Query Frame = 1 
Query:   13 KGKVTTRQVLRLHPDLAPFRAAVLPLSKKPQDLIDVASDLHQALLFNLGDVDYDIKGAIGRRYRRHDEIGTPFCITVDHDSLQDNEVTVRFRDSSKQTRVPID 321
            KG V  R VLRL P LAP +AAVLPLSKKP+ L  VA DL Q L  N   +DYD  GAIGRRYRR DEIGTP CITVD D+L D  VT+R RD+ +Q RV ++
Sbjct:    2 KGGVDKRTVLRLDPRLAPVKAAVLPLSKKPE-LQSVAQDLAQDLRLNDWMIDYDESGAIGRRYRRQDEIGTPLCITVDFDTLDDQAVTIRERDTMQQERVALN 103
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BLAST of mRNA_M-pyrifera_M_contig88027.20449.1 vs. uniprot
Match: A0A358C171_9BACT (Glycine--tRNA ligase (Fragment) n=1 Tax=Candidatus Uhrbacteria bacterium TaxID=2053634 RepID=A0A358C171_9BACT)

HSP 1 Score: 115 bits (288), Expect = 1.030e-30
Identity = 60/104 (57.69%), Postives = 80/104 (76.92%), Query Frame = 1 
Query:    7 DAKGKVTTRQVLRLHPDLAPFRAAVLPLSKKPQDLIDVASDLHQALLFNLGDVDYDIKGAIGRRYRRHDEIGTPFCITVDHDSLQDNEVTVRFRDSSKQTRVPI 318
            D+KG V TR V++L P++AP++ AVLPLSKK Q LI  A  ++  LL N   V++D+ G+IG+RYRR DEIGTPFC+TVD ++L+D  VTVR RD+ +QTRVPI
Sbjct:   38 DSKGGVDTRVVMKLTPEMAPYQVAVLPLSKKDQ-LIAPAQVIYNTLL-NTMRVEFDLSGSIGKRYRRQDEIGTPFCVTVDFETLEDKAVTVRDRDTMQQTRVPI 139
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BLAST of mRNA_M-pyrifera_M_contig88027.20449.1 vs. uniprot
Match: A0A0W8IFB1_9MICC (Glycine--tRNA ligase n=10 Tax=Nesterenkonia TaxID=57494 RepID=A0A0W8IFB1_9MICC)

HSP 1 Score: 119 bits (298), Expect = 1.480e-29
Identity = 64/105 (60.95%), Postives = 77/105 (73.33%), Query Frame = 1 
Query:    7 DAKGKVTTRQVLRLHPDLAPFRAAVLPLSKKPQDLIDVASDLHQALLFNLGDVDYDIKGAIGRRYRRHDEIGTPFCITVDHDSLQDNEVTVRFRDSSKQTRVPID 321
            + KG V TR VL+LHP LAP +AAVLPLSKKP+ L  V+ DL Q+L  +   +D+D  GAIGRRYRR DEIGTPFCITVD D+L+D  VT+R RD   Q RV +D
Sbjct:  346 NTKGGVDTRTVLKLHPKLAPVKAAVLPLSKKPE-LAGVSQDLAQSLRQHWN-IDFDDSGAIGRRYRRQDEIGTPFCITVDFDTLEDQAVTIRDRDEMTQERVSLD 448
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BLAST of mRNA_M-pyrifera_M_contig88027.20449.1 vs. uniprot
Match: A0A6J4UM06_9CHLR (Glycine--tRNA ligase n=1 Tax=uncultured Thermomicrobiales bacterium TaxID=1645740 RepID=A0A6J4UM06_9CHLR)

HSP 1 Score: 119 bits (298), Expect = 1.650e-29
Identity = 63/106 (59.43%), Postives = 76/106 (71.70%), Query Frame = 1 
Query:    4 VDAKGKVTTRQVLRLHPDLAPFRAAVLPLSKKPQDLIDVASDLHQALLFNLGD-VDYDIKGAIGRRYRRHDEIGTPFCITVDHDSLQDNEVTVRFRDSSKQTRVPI 318
            VD  GK  TR VLRLHP LAP++AAVLPL+KKP+ L +VA  L   L    G  +DYD    IG+RYRR DEIGTPFC+TVD D+LQD  VT+R RD++ Q RVP+
Sbjct:  355 VDVNGKPDTRVVLRLHPRLAPYKAAVLPLAKKPE-LTEVAKTLFDTLEAETGALIDYDETANIGKRYRRQDEIGTPFCVTVDFDTLQDRAVTIRDRDTTNQERVPL 459
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BLAST of mRNA_M-pyrifera_M_contig88027.20449.1 vs. uniprot
Match: A0A7W0SAT2_9CHLR (Glycine--tRNA ligase n=1 Tax=Chloroflexia bacterium TaxID=2448782 RepID=A0A7W0SAT2_9CHLR)

HSP 1 Score: 114 bits (284), Expect = 1.730e-29
Identity = 61/106 (57.55%), Postives = 75/106 (70.75%), Query Frame = 1 
Query:    4 VDAKGKVTTRQVLRLHPDLAPFRAAVLPLSKKPQDLIDVASDLHQALLFNLGD-VDYDIKGAIGRRYRRHDEIGTPFCITVDHDSLQDNEVTVRFRDSSKQTRVPI 318
            VD  GK  TR +LRLHP LAP++AAVLPL KKP+ L +VA  L   L    G  VDYD  G IG+RYRR DEIGTPFC T+D+++L D  VTVR RD++ Q R+P+
Sbjct:   88 VDVNGKPDTRVLLRLHPRLAPYKAAVLPLMKKPE-LAEVARSLFTRLEDETGALVDYDETGNIGKRYRRQDEIGTPFCFTIDYETLDDQAVTVRDRDTTAQERLPL 192
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BLAST of mRNA_M-pyrifera_M_contig88027.20449.1 vs. uniprot
Match: A0A259S9Q3_9MICO (Glycine--tRNA ligase (Fragment) n=1 Tax=Microbacterium sp. 14-71-5 TaxID=1970400 RepID=A0A259S9Q3_9MICO)

HSP 1 Score: 116 bits (290), Expect = 2.330e-29
Identity = 65/106 (61.32%), Postives = 74/106 (69.81%), Query Frame = 1 
Query:   10 AKGKVTTRQVLRLHPDLAPFRAAVLPLSKKPQDLIDVASDLHQALLFNLGDVDYDIKGAIGRRYRRHDEIGTPFCITVDHDSLQDNEVTVRFRDSSKQTRVPIDLL 327
            A GK  TR VLRL P LAPF  AVLPLSKK   L+ VA ++   LL    DVDYD   AIGRRYRR DE+GTP+C+TVD DSL D  VTVR RD+ +Q R+PID L
Sbjct:  195 ADGKADTRTVLRLDPRLAPFTIAVLPLSKK-DTLVPVAQEVAD-LLREFWDVDYDDAQAIGRRYRRQDEVGTPYCVTVDFDSLGDRAVTVRDRDTMQQVRIPIDQL 298
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BLAST of mRNA_M-pyrifera_M_contig88027.20449.1 vs. uniprot
Match: A0A7V8VWF9_9CHLR (Glycine--tRNA ligase n=1 Tax=Chloroflexia bacterium TaxID=2448782 RepID=A0A7V8VWF9_9CHLR)

HSP 1 Score: 112 bits (279), Expect = 3.700e-29
Identity = 63/106 (59.43%), Postives = 74/106 (69.81%), Query Frame = 1 
Query:    4 VDAKGKVTTRQVLRLHPDLAPFRAAVLPLSKKPQDLIDVASDLHQALLFNLGD-VDYDIKGAIGRRYRRHDEIGTPFCITVDHDSLQDNEVTVRFRDSSKQTRVPI 318
            VD  GK  TR VLRL P LAP++AAVLPL+KKP+ L  VA +L   L  +L   VDYD    IG+RYRR DEIGTPFC+TVD DSL+D  VTVR RD+  Q R+ I
Sbjct:   51 VDVNGKADTRVVLRLAPRLAPYKAAVLPLAKKPE-LTTVARELFTTLQDDLDTLVDYDETANIGKRYRRQDEIGTPFCLTVDFDSLEDGAVTVRDRDTMAQERIAI 155
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BLAST of mRNA_M-pyrifera_M_contig88027.20449.1 vs. uniprot
Match: UPI00182E9A4A (Glycine--tRNA ligase n=1 Tax=Streptomyces sp. TaxID=1931 RepID=UPI00182E9A4A)

HSP 1 Score: 113 bits (283), Expect = 3.940e-29
Identity = 63/105 (60.00%), Postives = 75/105 (71.43%), Query Frame = 1 
Query:    7 DAKGKVTTRQVLRLHPDLAPFRAAVLPLSKKPQDLIDVASDLHQALLFNLGDVDYDIKGAIGRRYRRHDEIGTPFCITVDHDSLQDNEVTVRFRDSSKQTRVPID 321
            +AKGK+  R VLRL P LAP + AVLPLS+ P+ L   A  L QAL  +   +D+D  GAIGRRYRR DEIGTP+C+TVD D+L DN VTVR RDS KQ RV +D
Sbjct:  108 NAKGKMEKRTVLRLDPRLAPVKVAVLPLSRNPE-LSPKAKGLAQALRQHWN-IDFDDAGAIGRRYRRQDEIGTPYCVTVDFDTLDDNAVTVRERDSMKQERVSLD 210
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BLAST of mRNA_M-pyrifera_M_contig88027.20449.1 vs. uniprot
Match: A0A3D4QWD1_9FIRM (Glycine--tRNA ligase (Fragment) n=1 Tax=Clostridiales bacterium TaxID=1898207 RepID=A0A3D4QWD1_9FIRM)

HSP 1 Score: 109 bits (272), Expect = 7.330e-29
Identity = 57/97 (58.76%), Postives = 74/97 (76.29%), Query Frame = 1 
Query:   31 RQVLRLHPDLAPFRAAVLPLSKKPQDLIDVASDLHQALLFNLGDVDYDIKGAIGRRYRRHDEIGTPFCITVDHDSLQDNEVTVRFRDSSKQTRVPID 321
            R V++LHP LAPF+AAVLPLSKK   L + A +L+ +L+     +DYD  G+IG+RYRR DEIGTP+CITVD D+L+D  VTVR RD+ +Q R+PID
Sbjct:    1 RVVMKLHPALAPFQAAVLPLSKK---LKEPAEELYSSLVGKFN-IDYDETGSIGKRYRREDEIGTPYCITVDFDTLEDQAVTVRDRDTMEQIRLPID 93
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig88027.20449.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7W0V787_9CHLR4.450e-3159.05Glycine--tRNA ligase n=1 Tax=Chloroflexia bacteriu... [more]
UPI00199C2E9E9.340e-3162.14His/Gly/Thr/Pro-type tRNA ligase C-terminal domain... [more]
A0A358C171_9BACT1.030e-3057.69Glycine--tRNA ligase (Fragment) n=1 Tax=Candidatus... [more]
A0A0W8IFB1_9MICC1.480e-2960.95Glycine--tRNA ligase n=10 Tax=Nesterenkonia TaxID=... [more]
A0A6J4UM06_9CHLR1.650e-2959.43Glycine--tRNA ligase n=1 Tax=uncultured Thermomicr... [more]
A0A7W0SAT2_9CHLR1.730e-2957.55Glycine--tRNA ligase n=1 Tax=Chloroflexia bacteriu... [more]
A0A259S9Q3_9MICO2.330e-2961.32Glycine--tRNA ligase (Fragment) n=1 Tax=Microbacte... [more]
A0A7V8VWF9_9CHLR3.700e-2959.43Glycine--tRNA ligase n=1 Tax=Chloroflexia bacteriu... [more]
UPI00182E9A4A3.940e-2960.00Glycine--tRNA ligase n=1 Tax=Streptomyces sp. TaxI... [more]
A0A3D4QWD1_9FIRM7.330e-2958.76Glycine--tRNA ligase (Fragment) n=1 Tax=Clostridia... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig88027contigM-pyrifera_M_contig88027:39..365 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score121.3
Seed ortholog evalue2.8e-25
Seed eggNOG ortholog641146.HMPREF9020_00398
Preferred nameglyQS
KEGG rclassRC00055,RC00523
KEGG koko:K01880
KEGG ReactionR03654
KEGG Pathwayko00970,map00970
KEGG ModuleM00359,M00360
Hectar predicted targeting categoryother localisation
GOsGO:0000166,GO:0000287,GO:0003674,GO:0003824,GO:0004812,GO:0004820,GO:0005488,GO:0005515,GO:0005524,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0006082,GO:0006139,GO:0006399,GO:0006412,GO:0006418,GO:0006426,GO:0006518,GO:0006520,GO:0006725,GO:0006807,GO:0008144,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009345,GO:0009987,GO:0010467,GO:0016070,GO:0016594,GO:0016597,GO:0016874,GO:0016875,GO:0017076,GO:0019538,GO:0019752,GO:0030554,GO:0031406,GO:0032553,GO:0032555,GO:0032559,GO:0032991,GO:0034641,GO:0034645,GO:0034660,GO:0035639,GO:0036094,GO:0042165,GO:0042802,GO:0042803,GO:0043038,GO:0043039,GO:0043043,GO:0043167,GO:0043168,GO:0043169,GO:0043170,GO:0043177,GO:0043436,GO:0043603,GO:0043604,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044271,GO:0044281,GO:0044424,GO:0044444,GO:0044464,GO:0046483,GO:0046872,GO:0046983,GO:0071704,GO:0090304,GO:0097159,GO:0097367,GO:0140098,GO:0140101,GO:1901265,GO:1901360,GO:1901363,GO:1901564,GO:1901566,GO:1901576,GO:1902494
EggNOG free text desc.Catalyzes the attachment of glycine to tRNA(Gly)
EggNOG OGs2GIT3@201174,4CYZU@85004,COG0423@1,COG0423@2
Ec32 ortholog descriptionGlycyl-tRNA Synthetase (C-terminal region)
Ec32 orthologEc-07_006780.1
EC6.1.1.14
COG Functional cat.J
BiGG ReactioniSB619.SA_RS07880
Best tax levelBifidobacteriales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko01007,ko03016,ko03029
Exons1
Model size327
Cds size327
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816789.8677914-CDS-M-pyrifera_M_contig88027:38..3651622816789.8677914-CDS-M-pyrifera_M_contig88027:38..365Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig88027 39..365 +
1692278179.7653472-CDS-M-pyrifera_M_contig88027:38..3651692278179.7653472-CDS-M-pyrifera_M_contig88027:38..365Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig88027 39..365 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig88027.20449.1prot_M-pyrifera_M_contig88027.20449.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig88027 39..365 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig88027.20449.1

>prot_M-pyrifera_M_contig88027.20449.1 ID=prot_M-pyrifera_M_contig88027.20449.1|Name=mRNA_M-pyrifera_M_contig88027.20449.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=109bp
MVDAKGKVTTRQVLRLHPDLAPFRAAVLPLSKKPQDLIDVASDLHQALLF
NLGDVDYDIKGAIGRRYRRHDEIGTPFCITVDHDSLQDNEVTVRFRDSSK
QTRVPIDLL
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mRNA from alignment at M-pyrifera_M_contig88027:39..365+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig88027.20449.1 ID=mRNA_M-pyrifera_M_contig88027.20449.1|Name=mRNA_M-pyrifera_M_contig88027.20449.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=327bp|location=Sequence derived from alignment at M-pyrifera_M_contig88027:39..365+ (Macrocystis pyrifera P11B4 male)
ATGGTGGATGCCAAAGGAAAAGTAACAACTCGTCAAGTTCTGCGACTCCA
CCCTGACCTTGCTCCATTTCGTGCAGCTGTTCTACCCCTTTCAAAGAAAC
CACAAGACCTCATAGACGTTGCTTCAGATCTGCACCAAGCGCTTTTATTC
AACCTTGGAGATGTAGACTATGACATCAAAGGTGCGATTGGTAGAAGATA
CAGGAGACACGATGAAATTGGAACTCCATTTTGCATCACTGTTGACCACG
ATTCCCTCCAAGACAACGAAGTTACAGTCCGTTTTCGCGATTCATCGAAA
CAAACTCGTGTTCCCATCGATCTTCTC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig88027:39..365+

>mRNA_M-pyrifera_M_contig88027.20449.1 ID=mRNA_M-pyrifera_M_contig88027.20449.1|Name=mRNA_M-pyrifera_M_contig88027.20449.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=654bp|location=Sequence derived from alignment at M-pyrifera_M_contig88027:39..365+ (Macrocystis pyrifera P11B4 male)
ATGGTGGATGCCAAAGGAAAAGTAACAACTCGTCAAGTTCTGCGACTCCA
CCCTGACCTTGCTCCATTTCGTGCAGCTGTTCTACCCCTTTCAAAGAAAC
CACAAGACCTCATAGACGTTGCTTCAGATCTGCACCAAGCGCTTTTATTC
AACCTTGGAGATGTAGACTATGACATCAAAGGTGCGATTGGTAGAAGATA
CAGGAGACACGATGAAATTGGAACTCCATTTTGCATCACTGTTGACCACG
ATTCCCTCCAAGACAACGAAGTTACAGTCCGTTTTCGCGATTCATCGAAA
CAAACTCGTGTTCCCATCGATCTTCTCATGGTGGATGCCAAAGGAAAAGT
AACAACTCGTCAAGTTCTGCGACTCCACCCTGACCTTGCTCCATTTCGTG
CAGCTGTTCTACCCCTTTCAAAGAAACCACAAGACCTCATAGACGTTGCT
TCAGATCTGCACCAAGCGCTTTTATTCAACCTTGGAGATGTAGACTATGA
CATCAAAGGTGCGATTGGTAGAAGATACAGGAGACACGATGAAATTGGAA
CTCCATTTTGCATCACTGTTGACCACGATTCCCTCCAAGACAACGAAGTT
ACAGTCCGTTTTCGCGATTCATCGAAACAAACTCGTGTTCCCATCGATCT
TCTC
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