mRNA_M-pyrifera_M_contig84843.19771.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig84843.19771.1
Unique NamemRNA_M-pyrifera_M_contig84843.19771.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig84843.19771.1 vs. uniprot
Match: A0A7S2RUW1_9STRA (Hypothetical protein (Fragment) n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2RUW1_9STRA)

HSP 1 Score: 85.9 bits (211), Expect = 2.470e-16
Identity = 66/212 (31.13%), Postives = 93/212 (43.87%), Query Frame = 1
Query:   46 AHRSAVTSLRFEDGGLRLASADAAGHVVVWRWRWRDSKDAFTSPSAPESYVPVDITHPSVQDTPIVDIAWRPG----------------RPQLVVCGHRNLLRLFDVGPKSLQPVRAFPSLSNAASHLTIAFSPDGRLLAAGGDSGTLTIWDVDSGVVLLGSARATHAGARIAFPSPLGSVHWSPTEHLVAATSFGDPNPVLLCEPKKRQPV 633
            AH + V +L  +    R+ S DA G  VVW WRW  S +   S   PE      I  P +Q   I  +A RP                 R QL++      L L+D+  ++   V  +       S L   FSPDGRL+ AGG+ G++ IWD  SG  L       H  + + F  P+  + W+P +H VA  + G   PVL+   +K   V
Sbjct:   42 AHHTHVNALAIDTDHHRVYSGDAEG--VVWSWRWESSGEDGASDFRPERA----IQPPKLQGRAITSLAMRPKLRVTGASNKGSEVTRRRNQLLIYSQGGFLILYDMSTRT--EVVQYRGAVCKNSRLPATFSPDGRLVLAGGEDGSVGIWDAWSGDSL------HHVFSEVRFTGPIMDISWNPRQHCVALAACGGNFPVLVGYARKNTAV 239          
BLAST of mRNA_M-pyrifera_M_contig84843.19771.1 vs. uniprot
Match: A0A3P3YLQ6_PLABS (Uncharacterized protein n=1 Tax=Plasmodiophora brassicae TaxID=37360 RepID=A0A3P3YLQ6_PLABS)

HSP 1 Score: 75.9 bits (185), Expect = 2.830e-12
Identity = 51/187 (27.27%), Postives = 89/187 (47.59%), Query Frame = 1
Query:   49 HRSAVTSLRFEDGGLRLASADAAGHVVVWRWRWRDSKDAFTSPSAPESYVPVD-ITHPSVQDTPIVDIAWRPGRPQLVVCGHRNLLRLFDVGPKSLQPVRAFPSLSNAASHLTIAFSPDGRLLAAGGDSGTLTIWDVDSGVVLLGSARATHAGARIAFPSPLGSVHWSPTEHLVAATSFGDPNPVLL 606
            H + + S+  +  GLR+ + DA+G V  W       +D    P +   + PV  + HP ++D  I  +   P R  +VV    +LL +F V    L+ V +     ++A  +   FSPDG  + +G + G + +W V SG ++        +     + +    V W P++HL+AA SFG   P+++
Sbjct:  576 HDAPINSIAVDQQGLRMWTGDASGSVKSW------DRDL---PQSASRFRPVHRLQHPELKDVAITSVRHDPVRDSIVVMARNHLLYVFGVRRYDLRRVLSGVQCDHSA--IRAQFSPDGAFVVSGSEDGKVHVWRVASGELV--------SQIETGYTAACYGVAWHPSQHLIAACSFGGDYPIMV 743          
BLAST of mRNA_M-pyrifera_M_contig84843.19771.1 vs. uniprot
Match: D8LM44_ECTSI (Similar to jouberin isoform 1 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LM44_ECTSI)

HSP 1 Score: 73.6 bits (179), Expect = 1.760e-11
Identity = 55/188 (29.26%), Postives = 82/188 (43.62%), Query Frame = 1
Query:   49 HRSAVTSLRFEDGGLRLASADAAGHVVVWRWRWRDSKDAFTSPSAPESYVPVDITHPSVQDTPIVDIAWRPG--RPQLVVCGHRNLLRLFDVGPKSLQPVRAFPSLSNAASHLTIAFSPDGRLLAAGGDSGTLTIWDVDSGVVLLGSARATHAGARIAFPSPLGSVHWSPTEHLVAATSFGDPNPVLL 606
            H S V ++ ++    RL S D AG +VVWR      +         +  +   + H  +    I  +A  P   R QL+V  H N LRL D+G   L     F   + A S +  +FSPDGR + +G ++G + +W+   G       R       +     L  V W P +H+VA   +G   PVLL
Sbjct:  521 HESHVNAIVYDSRNSRLYSGDGAGVIVVWR------RGGGGQGGVEDYGILRKVQHADLIGKAITSLALAPRLRRGQLLVQAHGNTLRLLDLGTYRLVNP-GFAGGATAKSLIRASFSPDGRFVVSGSETGRVRVWESQEG------KRVKTPLQTLGTACCLRGVGWHPNQHVVALAGYGQDAPVLL 695          
BLAST of mRNA_M-pyrifera_M_contig84843.19771.1 vs. uniprot
Match: A2G3I2_TRIVA (Uncharacterized protein n=1 Tax=Trichomonas vaginalis TaxID=5722 RepID=A2G3I2_TRIVA)

HSP 1 Score: 58.5 bits (140), Expect = 2.040e-6
Identity = 45/197 (22.84%), Postives = 85/197 (43.15%), Query Frame = 1
Query:   49 HRSAVTSLRFEDGGLRLASADAAGHVVVWRWRWRDSKDAFTSPSAPESYVPVDITHPSVQDTPIVDIAWRPGRPQLVVCGHRNLLRLFDVGPKSLQPVRAFPSLSNAASHLTIAFSPDGRLLAAGGDSGTLTIWDVDSGVVLLGSARATHAGARIAFPSPLGSVHWSPTEHLVAATSFGDPNPVLLCEPKKRQPVPD 639
            ++  V S+ F   G  L S D++G + V+    R         S  +    +      ++D PI  ++       L+V    N++R+F+   K + P + +        ++   FSPD + + AG + GT  +W      ++              F  P+ +V W+P E++VA +SFG   P+++   K   P+P+
Sbjct:  397 NKGTVNSITFSPDGKTLYSGDSSGQICVFNTDIRGG-------SRHDIVRKLIAKDEEIKDVPITHLSMELSPYSLLVHTRDNVIRIFET--KVMVPSQRYTGTICTKFNIPSVFSPDCQFVLAGSEDGTAKVWAARKADII------ETPEWNFKFDYPVTAVAWNPVENMVAFSSFGPKQPIIIF--KDPTPLPE 576          
BLAST of mRNA_M-pyrifera_M_contig84843.19771.1 vs. uniprot
Match: A0A1J4K373_9EUKA (Uncharacterized protein n=1 Tax=Tritrichomonas foetus TaxID=1144522 RepID=A0A1J4K373_9EUKA)

HSP 1 Score: 57.0 bits (136), Expect = 6.710e-6
Identity = 42/187 (22.46%), Postives = 78/187 (41.71%), Query Frame = 1
Query:   49 HRSAVTSLRFEDGGLRLASADAAGHVVVWRWRWR-DSKDAFTSPSAPESYVPVDITHPSVQDTPIVDIAWRPGRPQLVVCGHRNLLRLFDVGPKSLQPVRAFPSLSNAASHLTIAFSPDGRLLAAGGDSGTLTIWDVDSGVVLLGSARATHAGARIAFPSPLGSVHWSPTEHLVAATSFGDPNPVLL 606
            H+  + SL F   G  L + DA G +  W      D  D F             +    ++   I  +        L+V    N++R+F+   K + P + +  +      +   FSPDG+ + +G + G++ +W V     +  +           F  P+ +V W+  E++VA +SFG+  P+L+
Sbjct:  393 HQVRINSLTFSPDGKALYAGDADGIISSWNTNLEPDGIDGFRRIKL--------VKEGEIEKVSITHVEMGKSNFSLLVHTQDNMIRIFET--KVMVPSQRYSGILCKKFRMISTFSPDGQYILSGSEDGSVMLWTVRKAEPVAVNEWTCK------FDQPVTAVAWNRVENMVAFSSFGESQPILV 563          
BLAST of mRNA_M-pyrifera_M_contig84843.19771.1 vs. uniprot
Match: A0A5N4AHG3_PHOPY (Uncharacterized protein n=3 Tax=Photinus pyralis TaxID=7054 RepID=A0A5N4AHG3_PHOPY)

HSP 1 Score: 56.6 bits (135), Expect = 9.230e-6
Identity = 53/189 (28.04%), Postives = 89/189 (47.09%), Query Frame = 1
Query:   49 HRSAVTSLRFEDGGLRLASADAAGHVVVWRWRWRDSKDAFTSPSAPESYVPVDITHPSVQDTPIVDIAWRPGRPQLVVCGHRNLLRLFDVGPKSLQPVRAFPSLSNAASHLTIAFSPDGRLLAAGGDSGTLTIWDVDSGVVLLGSARATHAGARIAFPSPLGSVH---WSPTEHLVAATSFGDPNPVLL 606
            H+  VTSL F      L SAD+ G V+ WR R  DS   +   S    Y  +D+     +DT I  I       +L++    + LRL D+  K+   ++     +N       +FSP G  + AGG++GT+++W+ D     LG   AT+      F     ++H   + P ++++A + +G   P+L+
Sbjct:  386 HKGYVTSLCFASNHHSLYSADSVGIVIEWR-RGEDSHHLW---SFSREYKWIDL-----KDTIINQIVLHKRERRLLIHARDSTLRLVDL--KTGCTLQWLQGETNNRFRTICSFSPCGTYIFAGGENGTVSVWNAD-----LGDLIATYEP----FNKQRVTIHCVQFHPHDNILAISHYGHNVPILI 554          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig84843.19771.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 6
Match NameE-valueIdentityDescription
A0A7S2RUW1_9STRA2.470e-1631.13Hypothetical protein (Fragment) n=1 Tax=Rhizochrom... [more]
A0A3P3YLQ6_PLABS2.830e-1227.27Uncharacterized protein n=1 Tax=Plasmodiophora bra... [more]
D8LM44_ECTSI1.760e-1129.26Similar to jouberin isoform 1 n=1 Tax=Ectocarpus s... [more]
A2G3I2_TRIVA2.040e-622.84Uncharacterized protein n=1 Tax=Trichomonas vagina... [more]
A0A1J4K373_9EUKA6.710e-622.46Uncharacterized protein n=1 Tax=Tritrichomonas foe... [more]
A0A5N4AHG3_PHOPY9.230e-628.04Uncharacterized protein n=3 Tax=Photinus pyralis T... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig84843contigM-pyrifera_M_contig84843:195..869 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score76.3
Seed ortholog evalue2.1e-11
Seed eggNOG ortholog44056.XP_009041104.1
Preferred nameAHI1
KEGG koko:K11143,ko:K16740
KEGG Pathwayko05016,map05016
Hectar predicted targeting categoryother localisation
GOsGO:0001654,GO:0001655,GO:0001738,GO:0001750,GO:0001754,GO:0001822,GO:0001947,GO:0002009,GO:0002090,GO:0002092,GO:0002791,GO:0003002,GO:0003007,GO:0003143,GO:0003407,GO:0003674,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005813,GO:0005814,GO:0005815,GO:0005829,GO:0005856,GO:0005911,GO:0005912,GO:0005929,GO:0006355,GO:0006357,GO:0006810,GO:0006903,GO:0006996,GO:0007154,GO:0007165,GO:0007166,GO:0007167,GO:0007169,GO:0007275,GO:0007368,GO:0007389,GO:0007399,GO:0007417,GO:0007420,GO:0007423,GO:0007507,GO:0008104,GO:0008150,GO:0009605,GO:0009653,GO:0009790,GO:0009798,GO:0009799,GO:0009855,GO:0009887,GO:0009888,GO:0009889,GO:0009891,GO:0009893,GO:0009987,GO:0009991,GO:0010468,GO:0010556,GO:0010557,GO:0010604,GO:0010628,GO:0010720,GO:0010827,GO:0010829,GO:0010842,GO:0010941,GO:0010975,GO:0010976,GO:0015630,GO:0016043,GO:0016192,GO:0019219,GO:0019222,GO:0021510,GO:0022008,GO:0022406,GO:0022603,GO:0022607,GO:0023052,GO:0030030,GO:0030031,GO:0030054,GO:0030100,GO:0030154,GO:0030182,GO:0030856,GO:0030858,GO:0030860,GO:0030862,GO:0030902,GO:0031323,GO:0031325,GO:0031326,GO:0031328,GO:0031344,GO:0031346,GO:0031667,GO:0032094,GO:0032501,GO:0032502,GO:0032879,GO:0032880,GO:0032991,GO:0033036,GO:0033365,GO:0034613,GO:0034762,GO:0034763,GO:0035050,GO:0035239,GO:0035295,GO:0035844,GO:0035845,GO:0035869,GO:0036038,GO:0036064,GO:0039007,GO:0039008,GO:0039019,GO:0039020,GO:0039022,GO:0039023,GO:0040008,GO:0042221,GO:0042461,GO:0042462,GO:0042670,GO:0042802,GO:0042981,GO:0042995,GO:0043005,GO:0043010,GO:0043066,GO:0043067,GO:0043069,GO:0043226,GO:0043228,GO:0043229,GO:0043232,GO:0043583,GO:0044085,GO:0044422,GO:0044424,GO:0044430,GO:0044441,GO:0044444,GO:0044446,GO:0044450,GO:0044463,GO:0044464,GO:0044782,GO:0045595,GO:0045597,GO:0045664,GO:0045666,GO:0045807,GO:0045893,GO:0045927,GO:0045935,GO:0045944,GO:0046324,GO:0046325,GO:0046530,GO:0046548,GO:0046549,GO:0048259,GO:0048260,GO:0048468,GO:0048513,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048546,GO:0048562,GO:0048565,GO:0048568,GO:0048592,GO:0048593,GO:0048598,GO:0048646,GO:0048666,GO:0048699,GO:0048729,GO:0048731,GO:0048793,GO:0048839,GO:0048856,GO:0048869,GO:0050708,GO:0050767,GO:0050769,GO:0050789,GO:0050793,GO:0050794,GO:0050795,GO:0050896,GO:0051046,GO:0051049,GO:0051050,GO:0051051,GO:0051094,GO:0051128,GO:0051130,GO:0051171,GO:0051173,GO:0051179,GO:0051223,GO:0051234,GO:0051239,GO:0051240,GO:0051252,GO:0051254,GO:0051640,GO:0051641,GO:0051648,GO:0051649,GO:0051650,GO:0051656,GO:0051716,GO:0051960,GO:0051962,GO:0055123,GO:0060041,GO:0060042,GO:0060219,GO:0060255,GO:0060271,GO:0060284,GO:0060322,GO:0060429,GO:0060548,GO:0060562,GO:0060627,GO:0060972,GO:0060993,GO:0061326,GO:0061333,GO:0061371,GO:0065001,GO:0065007,GO:0070121,GO:0070161,GO:0070201,GO:0070727,GO:0070925,GO:0070986,GO:0071599,GO:0071840,GO:0072001,GO:0072006,GO:0072009,GO:0072028,GO:0072073,GO:0072078,GO:0072080,GO:0072088,GO:0072114,GO:0072176,GO:0072178,GO:0072359,GO:0080090,GO:0090087,GO:0090596,GO:0097458,GO:0097711,GO:0097730,GO:0097731,GO:0097733,GO:0120025,GO:0120031,GO:0120035,GO:0120036,GO:0120038,GO:0140056,GO:1902680,GO:1903506,GO:1903508,GO:1903530,GO:1905330,GO:1905332,GO:1905515,GO:1990403,GO:2000026,GO:2000027,GO:2000112,GO:2001141
EggNOG free text desc.WD repeat-containing protein
EggNOG OGsKOG0266@1,KOG0266@2759
COG Functional cat.B
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko03036,ko04812
Exons1
Model size675
Cds size675
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816755.6455936-CDS-M-pyrifera_M_contig84843:194..8691622816755.6455936-CDS-M-pyrifera_M_contig84843:194..869Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig84843 195..869 +
1692278159.3468466-CDS-M-pyrifera_M_contig84843:194..8691692278159.3468466-CDS-M-pyrifera_M_contig84843:194..869Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig84843 195..869 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig84843.19771.1prot_M-pyrifera_M_contig84843.19771.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig84843 195..869 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig84843.19771.1

>prot_M-pyrifera_M_contig84843.19771.1 ID=prot_M-pyrifera_M_contig84843.19771.1|Name=mRNA_M-pyrifera_M_contig84843.19771.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=225bp
GFASALGASGGGMGRAHRSAVTSLRFEDGGLRLASADAAGHVVVWRWRWR
DSKDAFTSPSAPESYVPVDITHPSVQDTPIVDIAWRPGRPQLVVCGHRNL
LRLFDVGPKSLQPVRAFPSLSNAASHLTIAFSPDGRLLAAGGDSGTLTIW
DVDSGVVLLGSARATHAGARIAFPSPLGSVHWSPTEHLVAATSFGDPNPV
LLCEPKKRQPVPDAARALAALGDL*
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mRNA from alignment at M-pyrifera_M_contig84843:195..869+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig84843.19771.1 ID=mRNA_M-pyrifera_M_contig84843.19771.1|Name=mRNA_M-pyrifera_M_contig84843.19771.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=675bp|location=Sequence derived from alignment at M-pyrifera_M_contig84843:195..869+ (Macrocystis pyrifera P11B4 male)
GGATTTGCTTCAGCGTTGGGTGCTTCTGGTGGTGGAATGGGTCGCGCCCA CCGATCTGCAGTGACTAGTTTGCGATTTGAGGATGGTGGGCTTCGTTTGG CGAGTGCAGATGCGGCGGGGCATGTGGTGGTGTGGCGTTGGCGTTGGCGC GATTCAAAAGATGCGTTTACGTCACCTTCTGCGCCTGAGAGCTATGTTCC AGTGGACATCACGCACCCTTCGGTGCAAGATACCCCAATTGTGGACATTG CCTGGCGTCCCGGGCGGCCGCAGCTGGTGGTGTGCGGCCATCGCAACTTG CTCCGGCTCTTTGACGTGGGTCCAAAGTCGCTTCAACCAGTGCGAGCCTT CCCTTCCCTCTCCAACGCCGCCTCCCACCTCACAATTGCATTCTCTCCCG ATGGGAGGCTCCTCGCGGCTGGAGGGGACTCAGGCACCCTCACCATCTGG GACGTTGATTCCGGAGTGGTGCTCCTCGGCTCTGCCAGAGCAACACACGC CGGCGCTCGCATCGCATTCCCATCCCCGCTCGGATCCGTCCACTGGAGCC CCACCGAACACCTCGTCGCAGCAACCTCGTTCGGAGACCCAAACCCAGTC CTCCTCTGCGAGCCAAAAAAGAGACAGCCAGTCCCAGACGCCGCAAGGGC GCTCGCAGCGCTTGGAGATCTATAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig84843:195..869+

>mRNA_M-pyrifera_M_contig84843.19771.1 ID=mRNA_M-pyrifera_M_contig84843.19771.1|Name=mRNA_M-pyrifera_M_contig84843.19771.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1350bp|location=Sequence derived from alignment at M-pyrifera_M_contig84843:195..869+ (Macrocystis pyrifera P11B4 male)
GGATTTGCTTCAGCGTTGGGTGCTTCTGGTGGTGGAATGGGTCGCGCCCA
CCGATCTGCAGTGACTAGTTTGCGATTTGAGGATGGTGGGCTTCGTTTGG
CGAGTGCAGATGCGGCGGGGCATGTGGTGGTGTGGCGTTGGCGTTGGCGC
GATTCAAAAGATGCGTTTACGTCACCTTCTGCGCCTGAGAGCTATGTTCC
AGTGGACATCACGCACCCTTCGGTGCAAGATACCCCAATTGTGGACATTG
CCTGGCGTCCCGGGCGGCCGCAGCTGGTGGTGTGCGGCCATCGCAACTTG
CTCCGGCTCTTTGACGTGGGTCCAAAGTCGCTTCAACCAGTGCGAGCCTT
CCCTTCCCTCTCCAACGCCGCCTCCCACCTCACAATTGCATTCTCTCCCG
ATGGGAGGCTCCTCGCGGCTGGAGGGGACTCAGGCACCCTCACCATCTGG
GACGTTGATTCCGGAGTGGTGCTCCTCGGCTCTGCCAGAGCAACACACGC
CGGCGCTCGCATCGCATTCCCATCCCCGCTCGGATCCGTCCACTGGAGCC
CCACCGAACACCTCGTCGCAGCAACCTCGTTCGGAGACCCAAACCCAGTC
CTCCTCTGCGAGCCAAAAAAGAGACAGCCAGTCCCAGACGCCGCAAGGGC
GCTCGCAGCGCTTGGAGATCTATAGGGATTTGCTTCAGCGTTGGGTGCTT
CTGGTGGTGGAATGGGTCGCGCCCACCGATCTGCAGTGACTAGTTTGCGA
TTTGAGGATGGTGGGCTTCGTTTGGCGAGTGCAGATGCGGCGGGGCATGT
GGTGGTGTGGCGTTGGCGTTGGCGCGATTCAAAAGATGCGTTTACGTCAC
CTTCTGCGCCTGAGAGCTATGTTCCAGTGGACATCACGCACCCTTCGGTG
CAAGATACCCCAATTGTGGACATTGCCTGGCGTCCCGGGCGGCCGCAGCT
GGTGGTGTGCGGCCATCGCAACTTGCTCCGGCTCTTTGACGTGGGTCCAA
AGTCGCTTCAACCAGTGCGAGCCTTCCCTTCCCTCTCCAACGCCGCCTCC
CACCTCACAATTGCATTCTCTCCCGATGGGAGGCTCCTCGCGGCTGGAGG
GGACTCAGGCACCCTCACCATCTGGGACGTTGATTCCGGAGTGGTGCTCC
TCGGCTCTGCCAGAGCAACACACGCCGGCGCTCGCATCGCATTCCCATCC
CCGCTCGGATCCGTCCACTGGAGCCCCACCGAACACCTCGTCGCAGCAAC
CTCGTTCGGAGACCCAAACCCAGTCCTCCTCTGCGAGCCAAAAAAGAGAC
AGCCAGTCCCAGACGCCGCAAGGGCGCTCGCAGCGCTTGGAGATCTATAG
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