mRNA_M-pyrifera_M_contig82299.19247.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig82299.19247.1
Unique NamemRNA_M-pyrifera_M_contig82299.19247.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig82299.19247.1 vs. uniprot
Match: A0A497U2V1_9BACT (DNA helicase n=1 Tax=bacterium endosymbiont of Escarpia laminata TaxID=1221601 RepID=A0A497U2V1_9BACT)

HSP 1 Score: 145 bits (367), Expect = 2.970e-38
Identity = 85/186 (45.70%), Postives = 115/186 (61.83%), Query Frame = 1
Query:    4 LNISRLTRGHGSIAQELDDAY-QANSMSGLRIFVDDDTTSLSGIVARASEWKHQHGIAALFVDYLGLVEVDGNEPRHVKLGQVSRTLKHFLFLLEIPIVAASQFNRESTKEDRRPRLSDLRDSGSIEQDADVVIALNPIADPDSDGRTL-------IELGVLKNRSGMSGWISDSVTFAGKTQRFL 537
            LN S L  G       L DA  +   +S L I++D DTT+L GI AR +EWK QHGIA   VD++GLVE   ++ R+  LG++SR+LK     L++PI+A SQ NR   ++ RRP L+DLRDSG+IEQDADV   L+       DG  +       +E+G+LKNR+G  GW+ +  TF G+TQ FL
Sbjct:  247 LNFSGLAYGDDEECTRLTDAMTEKPPISSLPIYIDTDTTTLGGITARMAEWKRQHGIALCIVDHIGLVEGGDDKTRNDWLGKISRSLKLTAKRLDLPILAVSQLNRSVERDKRRPVLADLRDSGNIEQDADVCAFLHV------DGNAVETTYGLPMEIGLLKNRTGQRGWLPEEFTFDGRTQTFL 426          
BLAST of mRNA_M-pyrifera_M_contig82299.19247.1 vs. uniprot
Match: UPI001FF07148 (DnaB-like helicase C-terminal domain-containing protein n=1 Tax=Flagellatimonas centrodinii TaxID=2806210 RepID=UPI001FF07148)

HSP 1 Score: 121 bits (304), Expect = 3.480e-29
Identity = 74/176 (42.05%), Postives = 105/176 (59.66%), Query Frame = 1
Query:   10 ISRLTRGHGSIAQELDDAYQANSMSG-LRIFVDDDTTSLSGIVARASEWKHQHGIAALFVDYLGLVEVDGNEPRHVKLGQVSRTLKHFLFLLEIPIVAASQFNRESTKEDRRPRLSDLRDSGSIEQDADVVIALNPIADPDSDGRTLIELGVLKNRSGMSGWISDSVTFAGKTQRF 534
            +SRL RG  S   E+  A  A +  G + +++D DT +L  I ARA+  K +HGI  + VD++GLVE      R+ ++G +SR+LK     L++P++A SQ NR    ++RRP L+DLRDSG+IEQDAD+V+ L+   D  S  R  I +G  KNR G + W      F   TQRF
Sbjct:  241 LSRLLRGTAS-PDEVSQATDAMTKLGDIPLWIDTDTYNLDAISARATVAKARHGIGWIAVDHMGLVETARMPSRNEQMGLISRSLKQLAKRLDVPVIAVSQLNRSCDTDNRRPGLADLRDSGNIEQDADIVMMLHCDKDDRSKNRRPISIGFPKNRVGPAVWPDADFEFDALTQRF 415          
BLAST of mRNA_M-pyrifera_M_contig82299.19247.1 vs. uniprot
Match: UPI00163E2CB6 (replicative DNA helicase n=2 Tax=Burkholderia gladioli TaxID=28095 RepID=UPI00163E2CB6)

HSP 1 Score: 108 bits (271), Expect = 2.290e-24
Identity = 72/189 (38.10%), Postives = 110/189 (58.20%), Query Frame = 1
Query:    1 GLNISRLTRGHGSIAQELDDAYQANS-MSGLRIFVDDDTT-SLSGIVARASEWKHQHGIAALFVDYLGLVEVDGNEPRHVKLGQVSRTLKHFLFLLEIPIVAASQFNRE-STKEDRRPRLSDLRDSGSIEQDADVVIAL--NPIADPDSDGRTLIELGVLKNRSGMSGWISDSVTFAGKTQRFLTEPLG 552
            G+N+  +  G     +E      A S M+G+ + VDD  + SL+ IV+R+   K +HG+A + VDYLGL+    +E   +K+G  S  LK     L++P++A +Q NR    + ++RP ++DLRDSG+IEQDADV++ L  + +  PDS  R   E+ + K R+G +G  S  V F G+ QRF   P G
Sbjct:  260 GINLGHILDGRRMTDEEWPRLTHAVSVMAGVTLLVDDTPSLSLAEIVSRSRTVKRKHGLALVIVDYLGLMTGGTDERHDLKIGSYSAGLKGLAKQLDVPVIALAQLNRGVENRPNKRPTMADLRDSGAIEQDADVILMLYRDQVYFPDSPDRGTAEIIIGKQRNGETG--SVRVAFEGEFQRFSDLPAG 446          
BLAST of mRNA_M-pyrifera_M_contig82299.19247.1 vs. uniprot
Match: A0A6L5F4G2_9ACTN (Replicative DNA helicase n=1 Tax=Acidimicrobiia bacterium TaxID=2080302 RepID=A0A6L5F4G2_9ACTN)

HSP 1 Score: 108 bits (270), Expect = 2.860e-24
Identity = 66/152 (43.42%), Postives = 98/152 (64.47%), Query Frame = 1
Query:   94 IFVDDD-TTSLSGIVARASEWKHQHGIAALFVDYLGLVEVDGNEPRHVKLGQVSRTLKHFLFLLEIPIVAASQFNRE-STKEDRRPRLSDLRDSGSIEQDADVVIAL--NPIADP-DSDGRTLIELGVLKNRSGMSGWISDSVTFAGKTQRF 534
            IF+DD    +++ I A++   K +HG+  + VDYL L++  G E R  ++ ++SR LK+    LE+PIVA SQ NR   ++ED+RPRLSDLR+SGSIEQDADVV+ +  +    P D++ R L E+ V K+R+G  G +   +TF  +  RF
Sbjct:  294 IFIDDSPVVTVTDIRAKSRRLKRRHGLGLVVVDYLQLMQASGRENRQQEIAEISRNLKNLARELEVPIVAVSQLNRSLESREDKRPRLSDLRESGSIEQDADVVMFIYRHEYYHPEDAEARGLAEVAVAKHRAGSVGTVK--MTFLPEFTRF 443          
BLAST of mRNA_M-pyrifera_M_contig82299.19247.1 vs. uniprot
Match: A0A383CK35_9ZZZZ (SF4 helicase domain-containing protein (Fragment) n=1 Tax=marine metagenome TaxID=408172 RepID=A0A383CK35_9ZZZZ)

HSP 1 Score: 101 bits (252), Expect = 1.810e-23
Identity = 60/147 (40.82%), Postives = 93/147 (63.27%), Query Frame = 1
Query:   70 ANSMSGLRIFVDDDTT-SLSGIVARASEWKHQHGIAALFVDYLGLVEVDGNEPRHVKLGQVSRTLKHFLFLLEIPIVAASQFNRES-TKEDRRPRLSDLRDSGSIEQDADVVIAL--NPIADPDSDGRTLIELGVLKNRSGMSGWIS 498
            A+ +  L I+ DD +  +++ I A++   K   GI  + VDYL L+E    + R  ++  +SR LK+    L++PI+A SQ NR +  +ED+RPRL DLR+SG+IEQDAD+V+ L  +   DP S+   + E+ ++KNRSG +G IS
Sbjct:   32 ASKVHSLPIYFDDASVITVTDIRAKSRRLKSMKGIDLIIVDYLQLMEDSSGDNRQQEIANISRNLKNLAKELDVPIIAVSQLNRAAEAREDKRPRLGDLRESGAIEQDADIVMMLYRDDYYDPASELTGIAEVNIVKNRSGETGKIS 178          
BLAST of mRNA_M-pyrifera_M_contig82299.19247.1 vs. uniprot
Match: A0A0U2SHR3_9BURK (DNA helicase n=2 Tax=Herbaspirillum TaxID=963 RepID=A0A0U2SHR3_9BURK)

HSP 1 Score: 104 bits (260), Expect = 7.430e-23
Identity = 78/209 (37.32%), Postives = 111/209 (53.11%), Query Frame = 1
Query:    1 GLNISRLTRGHGSIAQELDDA---------YQANSMSGLRIFVDDDTT-SLSGIVARASEWKHQHGIAALFVDYLGLVEVDGNEPRHVKLGQVSRTLKHFLFLLEIPIVAASQFNREST-KEDRRPRLSDLRDSGSIEQDADVVIALN--PIADPDSDG-RTLIELGVLKNRSGMSGWISDSVTFAGKTQRF-----LTEPLGPKRGAW 570
            GL++SR       I   +DDA         +  + ++ LR  +DD    SL  +  RA  WK +HG+  + VDYLGL+     E RH ++G  SR LK     L++ ++A +Q NR+S  + DRRP+LSDLRDSG IEQDADVV+ ++   + DP ++  R   E  V K R+G  G I   + F G T RF     L     P++  W
Sbjct:  252 GLSLSR-------ILGRIDDADAQAWGAVTHGCSRLNQLRFAIDDTAAISLLELRLRAKAWKRRHGLDLIVVDYLGLMTGGEGEKRHEQIGSYSRGLKALAKELDVTVLALAQLNRKSEDRADRRPQLSDLRDSGEIEQDADVVVLIHRPEMHDPTNEALRGFAEALVRKQRNGALGDIP--LKFKGATSRFEEWAGLIPTTTPQKSGW 451          
BLAST of mRNA_M-pyrifera_M_contig82299.19247.1 vs. uniprot
Match: A0A2E5X5T3_9BACT (Replicative DNA helicase n=2 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A2E5X5T3_9BACT)

HSP 1 Score: 103 bits (257), Expect = 2.020e-22
Identity = 67/172 (38.95%), Postives = 99/172 (57.56%), Query Frame = 1
Query:   70 ANSMSGLRIFVDDDT-TSLSGIVARASEWKHQHGIAALFVDYLGLVEVDGNEPRHVKLGQVSRTLKHFLFLLEIPIVAASQFNRE-STKEDRRPRLSDLRDSGSIEQDADVVIALNPIA--DPDSDGRTLIELGVLKNRSGMSGWISDSVTFAGKTQRF------LTEPLGP 555
            A  + G R+F+DD    S+  + +RA + KHQHG+  + +DYL L+     E R  ++  +SR LK     LEIP++A SQ +R   ++ED+RP+LSDLR+SGSIEQDADVV+ L      +P  + + L E    K R+G +G +   + F   T RF      + EP+ P
Sbjct:  293 AGELQGTRLFIDDTPGLSVMALRSRARKIKHQHGLDMVVIDYLQLMSHPKAESRQQEISAISRALKGLARELEIPVIALSQLSRAVESREDKRPQLSDLRESGSIEQDADVVLMLYRAEYYNPTDENKGLAEAICGKQRNGPTGRVK--LQFRASTMRFENREPSMAEPILP 462          
BLAST of mRNA_M-pyrifera_M_contig82299.19247.1 vs. uniprot
Match: UPI001906CD84 (replicative DNA helicase n=3 Tax=Burkholderia gladioli TaxID=28095 RepID=UPI001906CD84)

HSP 1 Score: 103 bits (257), Expect = 2.080e-22
Identity = 70/189 (37.04%), Postives = 105/189 (55.56%), Query Frame = 1
Query:    1 GLNISRLTRGHGSIAQELDDAYQANSMSGLRIFVDDDTT--SLSGIVARASEWKHQHGIAALFVDYLGLVEVDGNEPRHVKLGQVSRTLKHFLFLLEIPIVAASQFNRE-STKEDRRPRLSDLRDSGSIEQDADVVIAL--NPIADPDSDGRTLIELGVLKNRSGMSGWISDSVTFAGKTQRFLTEPLG 552
            G+N+  +  G     +E      A S+      + DDT   SL+ IV+R+   K +HG+  + VDYLGL+    +E   +K+G  S  LK     L++P++A +Q NR    + ++RP ++DLRDSG+IEQDADV++ L  + +  PDS  R   E+ V K R+G +G  S  V F G+ QRF   P G
Sbjct:  261 GINLGHVLDGRRMTDEEWPRLTHAVSVMAEMPMLVDDTPGLSLAEIVSRSRTVKRKHGLELIVVDYLGLMTGGTDERHDLKIGSYSAGLKGLAKQLDVPVIALAQLNRGVENRPNKRPTMADLRDSGAIEQDADVILMLYRDQVYHPDSTDRGTAEIIVGKQRNGETG--SVRVAFEGEFQRFSDLPAG 447          
BLAST of mRNA_M-pyrifera_M_contig82299.19247.1 vs. uniprot
Match: W0V8C2_9BURK (DNA helicase n=2 Tax=Janthinobacterium agaricidamnosum TaxID=55508 RepID=W0V8C2_9BURK)

HSP 1 Score: 103 bits (256), Expect = 2.770e-22
Identity = 66/153 (43.14%), Postives = 94/153 (61.44%), Query Frame = 1
Query:   97 FVDDDTTSLSGIVAR--ASEWKHQHGIAALFVDYLGLVEVDGNEPRHVKLGQVSRTLKHFLFLLEIPIVAASQFNRES-TKEDRRPRLSDLRDSGSIEQDADVVIALN--PIADPDSDG-RTLIELGVLKNRSGMSGWISDSVTFAGKTQRFL 537
            F  DDT +++ +  R  A  WK +HG+  + VDY+GL+     + RH ++G  SR+LK     L + I+A +Q NR+S  + D+RP+LSDLRDSG IEQDAD+VI ++   +  PDS   +   E+ V KNRSG  G I+  + F G T RFL
Sbjct:  288 FAIDDTAAITMLDLRMKAKAWKRKHGLGVIIVDYIGLMTGGDGDKRHEQIGYYSRSLKALAKELGVAIIALAQLNRQSEARTDKRPQLSDLRDSGEIEQDADIVILVHRPEMHHPDSPHLKGWAEVMVRKNRSGSLGTIN--MQFDGPTCRFL 438          
BLAST of mRNA_M-pyrifera_M_contig82299.19247.1 vs. uniprot
Match: UPI0018A0CF5A (replicative DNA helicase n=4 Tax=Clostridium paraputrificum TaxID=29363 RepID=UPI0018A0CF5A)

HSP 1 Score: 102 bits (254), Expect = 4.450e-22
Identity = 69/170 (40.59%), Postives = 99/170 (58.24%), Query Frame = 1
Query:   70 ANSMSGLRIFVDDDTTSLSGIVARASEWKHQHGIAALFVDYLGLVEV-DGNEPRHVKLGQVSRTLKHFLFLLEIPIVAASQFNRE-STKEDRRPRLSDLRDSGSIEQDADVVIAL--NPIADPDSDGRTLIELGVLKNRSGMS-----GWISDSVTFAGKTQRFLTEPLG 552
            AN ++  +IF+DD +T+LS I A+    K + G+  + +DYL L+E+ D    R  ++ ++SR LK     LEI IVA SQ +R    + D RP LSDLR+SGSIEQDADV+  L  +   + +S+ R + EL + KNR+G +     GWI       G+ QRF T   G
Sbjct:  274 ANVLAKRKIFIDDKSTNLSDIKAKCRSLKVKEGLDVVIIDYLQLIEISDKTNSREQEIAKISRELKKLAKKLEITIVALSQLSRAPEQRTDHRPVLSDLRESGSIEQDADVIFMLYRDEYYNKESEERNIAELIINKNRNGETKTIKLGWI-------GQYQRFATLDRG 436          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig82299.19247.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A497U2V1_9BACT2.970e-3845.70DNA helicase n=1 Tax=bacterium endosymbiont of Esc... [more]
UPI001FF071483.480e-2942.05DnaB-like helicase C-terminal domain-containing pr... [more]
UPI00163E2CB62.290e-2438.10replicative DNA helicase n=2 Tax=Burkholderia glad... [more]
A0A6L5F4G2_9ACTN2.860e-2443.42Replicative DNA helicase n=1 Tax=Acidimicrobiia ba... [more]
A0A383CK35_9ZZZZ1.810e-2340.82SF4 helicase domain-containing protein (Fragment) ... [more]
A0A0U2SHR3_9BURK7.430e-2337.32DNA helicase n=2 Tax=Herbaspirillum TaxID=963 RepI... [more]
A0A2E5X5T3_9BACT2.020e-2238.95Replicative DNA helicase n=2 Tax=Planctomycetes ba... [more]
UPI001906CD842.080e-2237.04replicative DNA helicase n=3 Tax=Burkholderia glad... [more]
W0V8C2_9BURK2.770e-2243.14DNA helicase n=2 Tax=Janthinobacterium agaricidamn... [more]
UPI0018A0CF5A4.450e-2240.59replicative DNA helicase n=4 Tax=Clostridium parap... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig82299contigM-pyrifera_M_contig82299:27..912 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score146.7
Seed ortholog evalue1.1e-32
Seed eggNOG ortholog1122604.JONR01000011_gene3585
Preferred namednaB
KEGG koko:K02314
KEGG Pathwayko03030,ko04112,map03030,map04112
Hectar predicted targeting categoryother localisation
EggNOG free text desc.it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with DnaC protein, primase, and other prepriming proteins
EggNOG OGs1MUG9@1224,1RPM2@1236,1X3P0@135614,COG0305@1,COG0305@2
EC3.6.4.12
COG Functional cat.L
Best tax levelXanthomonadales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03032
Exons2
Model size597
Cds size597
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816729.9069269-CDS-M-pyrifera_M_contig82299:26..761622816729.9069269-CDS-M-pyrifera_M_contig82299:26..76Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig82299 27..76 -
1692278144.6881013-CDS-M-pyrifera_M_contig82299:26..761692278144.6881013-CDS-M-pyrifera_M_contig82299:26..76Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig82299 27..76 -
1622816729.922264-CDS-M-pyrifera_M_contig82299:365..9121622816729.922264-CDS-M-pyrifera_M_contig82299:365..912Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig82299 366..912 -
1692278144.7019475-CDS-M-pyrifera_M_contig82299:365..9121692278144.7019475-CDS-M-pyrifera_M_contig82299:365..912Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig82299 366..912 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig82299.19247.1prot_M-pyrifera_M_contig82299.19247.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig82299 27..912 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig82299.19247.1

>prot_M-pyrifera_M_contig82299.19247.1 ID=prot_M-pyrifera_M_contig82299.19247.1|Name=mRNA_M-pyrifera_M_contig82299.19247.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=199bp
GLNISRLTRGHGSIAQELDDAYQANSMSGLRIFVDDDTTSLSGIVARASE
WKHQHGIAALFVDYLGLVEVDGNEPRHVKLGQVSRTLKHFLFLLEIPIVA
ASQFNRESTKEDRRPRLSDLRDSGSIEQDADVVIALNPIADPDSDGRTLI
ELGVLKNRSGMSGWISDSVTFAGKTQRFLTEPLGPKRGAWPWWREEKRN
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mRNA from alignment at M-pyrifera_M_contig82299:27..912-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig82299.19247.1 ID=mRNA_M-pyrifera_M_contig82299.19247.1|Name=mRNA_M-pyrifera_M_contig82299.19247.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=886bp|location=Sequence derived from alignment at M-pyrifera_M_contig82299:27..912- (Macrocystis pyrifera P11B4 male)
GGACTCAATATTTCCCGCTTGACCCGCGGTCACGGGAGCATCGCTCAGGA ACTTGATGACGCTTATCAGGCGAACTCGATGTCTGGTCTTCGCATCTTCG TCGATGACGACACCACGAGCTTGTCCGGGATCGTCGCGCGAGCTTCCGAG TGGAAGCACCAGCACGGGATAGCGGCACTTTTCGTCGACTATCTGGGACT GGTCGAAGTAGATGGCAACGAGCCACGACACGTGAAGCTTGGCCAGGTAT CGCGCACATTAAAGCATTTTTTATTTCTCCTTGAGATTCCGATCGTTGCA GCCTCGCAATTCAACCGCGAATCAACTAAGGAGGATCGGCGCCCGCGCCT TTCCGATTTACGCGACTCGGGCTCCATCGAGCAGGACGCCGATGTCGTGA TAGCCCTGAATCCGATTGCAGATCCGGACAGCGATGGTCGCACGTTAATC GAACTCGGGGTTTTGAAAAATCGTTCTGGAATGTCTGGTTGGATATCAGA CAGTGTCACCTTCGCCGGCAAAACCCAGAGGTTTCTGACCGAACCTCGTG GGCAACGATCGGCGTGATTCGCCGTACAGAAAACCCTGTCGTCATTTCGG CGCGGTTACCGTTGACGCTCAGTGGCTTGCGGACACGCCAATCGGTGTAC CAAAACCTGCCGTTATTCGAACGCGCGGGAGTTCAGGGTTGAGCGTTGCG CTTCGAAACATCGAGCGGCTACGTAGATTTGCTACGGGCGACCACGAGCG AGGGAGCGATGCAGAGTGGATAGCCGAGCGAATCAAGAAATACACCGCGT CCGCCGGGGCGCTAAGTCTTGACGAAGCGCTCGAAGTCGGACCGAAGCGA GGAGCCTGGCCGTGGTGGCGCGAGGAGAAGCGCAAT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig82299:27..912-

>mRNA_M-pyrifera_M_contig82299.19247.1 ID=mRNA_M-pyrifera_M_contig82299.19247.1|Name=mRNA_M-pyrifera_M_contig82299.19247.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1194bp|location=Sequence derived from alignment at M-pyrifera_M_contig82299:27..912- (Macrocystis pyrifera P11B4 male)
GGACTCAATATTTCCCGCTTGACCCGCGGTCACGGGAGCATCGCTCAGGA
ACTTGATGACGCTTATCAGGCGAACTCGATGTCTGGTCTTCGCATCTTCG
TCGATGACGACACCACGAGCTTGTCCGGGATCGTCGCGCGAGCTTCCGAG
TGGAAGCACCAGCACGGGATAGCGGCACTTTTCGTCGACTATCTGGGACT
GGTCGAAGTAGATGGCAACGAGCCACGACACGTGAAGCTTGGCCAGGTAT
CGCGCACATTAAAGCATTTTTTATTTCTCCTTGAGATTCCGATCGTTGCA
GCCTCGCAATTCAACCGCGAATCAACTAAGGAGGATCGGCGCCCGCGCCT
TTCCGATTTACGCGACTCGGGCTCCATCGAGCAGGACGCCGATGTCGTGA
TAGCCCTGAATCCGATTGCAGATCCGGACAGCGATGGTCGCACGTTAATC
GAACTCGGGGTTTTGAAAAATCGTTCTGGAATGTCTGGTTGGATATCAGA
CAGTGTCACCTTCGCCGGCAAAACCCAGAGGTTTCTGACCGAACCTCGGA
CTCAATATTTCCCGCTTGACCCGCGGTCACGGGAGCATCGCTCAGGAACT
TGATGACGCTTATCAGGCGAACTCGATGTCTGGTCTTCGCATCTTCGTCG
ATGACGACACCACGAGCTTGTCCGGGATCGTCGCGCGAGCTTCCGAGTGG
AAGCACCAGCACGGGATAGCGGCACTTTTCGTCGACTATCTGGGACTGGT
CGAAGTAGATGGCAACGAGCCACGACACGTGAAGCTTGGCCAGGTATCGC
GCACATTAAAGCATTTTTTATTTCTCCTTGAGATTCCGATCGTTGCAGCC
TCGCAATTCAACCGCGAATCAACTAAGGAGGATCGGCGCCCGCGCCTTTC
CGATTTACGCGACTCGGGCTCCATCGAGCAGGACGCCGATGTCGTGATAG
CCCTGAATCCGATTGCAGATCCGGACAGCGATGGTCGCACGTTAATCGAA
CTCGGGGTTTTGAAAAATCGTTCTGGAATGTCTGGTTGGATATCAGACAG
TGTCACCTTCGCCGGCAAAACCCAGAGGTTTCTGACCGAACCTCTCGGAC
CGAAGCGAGGAGCCTGGCCGTGGTGGCGCGAGGAGAAGCGCAATTCGGAC
CGAAGCGAGGAGCCTGGCCGTGGTGGCGCGAGGAGAAGCGCAAT
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