mRNA_M-pyrifera_M_contig126066.5266.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig126066.5266.1
Unique NamemRNA_M-pyrifera_M_contig126066.5266.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig126066.5266.1 vs. uniprot
Match: A0A2D8GI33_9ACTN (DNA topoisomerase (ATP-hydrolyzing) n=8 Tax=Acidimicrobiaceae bacterium TaxID=2024894 RepID=A0A2D8GI33_9ACTN)

HSP 1 Score: 312 bits (800), Expect = 1.550e-100
Identity = 158/170 (92.94%), Postives = 166/170 (97.65%), Query Frame = 1 
Query:    1 TVNAYARDRGFLKEKDDNLTGEDIREGLTAIISARIGDPQFEGQTKSKLGNVSMRSLVERVTNERMAEWLEEHPGEGKVIVTKATNAARARIAASDARKAIRSKSLLDGAGMPDKLKDCSAKDPERRELFIVEGDSAGGSAVRARDPETQAILPIRGKILNVERARIDKM 510
            TVN YARD+G LKEKDDNLTGEDIREGLTAIISARIG+PQFEGQTKSKLGNVSMRSLVE+VTNE+MAEWLEEHP EGK IVTKATNAARAR+AA+DARKAIRSKSLLDGAGMPDKLKDCSAK+PERRELFIVEGDSAGGSAVRARDPETQAILPIRGKILNVERAR+DKM
Sbjct:  343 TVNNYARDKGLLKEKDDNLTGEDIREGLTAIISARIGEPQFEGQTKSKLGNVSMRSLVEKVTNEKMAEWLEEHPAEGKAIVTKATNAARARVAAADARKAIRSKSLLDGAGMPDKLKDCSAKEPERRELFIVEGDSAGGSAVRARDPETQAILPIRGKILNVERARVDKM 512
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BLAST of mRNA_M-pyrifera_M_contig126066.5266.1 vs. uniprot
Match: A0A7X5WVW5_9ACTN (DNA topoisomerase (ATP-hydrolyzing) n=1 Tax=Actinomycetia bacterium TaxID=1883427 RepID=A0A7X5WVW5_9ACTN)

HSP 1 Score: 307 bits (787), Expect = 8.980e-99
Identity = 155/170 (91.18%), Postives = 165/170 (97.06%), Query Frame = 1 
Query:    1 TVNAYARDRGFLKEKDDNLTGEDIREGLTAIISARIGDPQFEGQTKSKLGNVSMRSLVERVTNERMAEWLEEHPGEGKVIVTKATNAARARIAASDARKAIRSKSLLDGAGMPDKLKDCSAKDPERRELFIVEGDSAGGSAVRARDPETQAILPIRGKILNVERARIDKM 510
            TVNAYAR++G LKEKD+NL GEDIREGLTAI+S RIG+PQFEGQTKSKLGNVSMRSLVERVTNER+AEWLEEHPGE K +VTKATNAARAR+AA+DARKAIRSKSLLDGAGMPDKLKDCSAK+PERRELFIVEGDSAGGSAVRARDPETQAILPIRGKILNVERARIDKM
Sbjct:  326 TVNAYAREKGTLKEKDENLQGEDIREGLTAIVSVRIGEPQFEGQTKSKLGNVSMRSLVERVTNERLAEWLEEHPGEAKALVTKATNAARARVAAADARKAIRSKSLLDGAGMPDKLKDCSAKEPERRELFIVEGDSAGGSAVRARDPETQAILPIRGKILNVERARIDKM 495
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BLAST of mRNA_M-pyrifera_M_contig126066.5266.1 vs. uniprot
Match: A0A7K1ZBL8_9ACTN (DNA topoisomerase (ATP-hydrolyzing) n=2 Tax=Acidimicrobiaceae bacterium TaxID=2024894 RepID=A0A7K1ZBL8_9ACTN)

HSP 1 Score: 305 bits (780), Expect = 9.960e-98
Identity = 154/170 (90.59%), Postives = 162/170 (95.29%), Query Frame = 1 
Query:    1 TVNAYARDRGFLKEKDDNLTGEDIREGLTAIISARIGDPQFEGQTKSKLGNVSMRSLVERVTNERMAEWLEEHPGEGKVIVTKATNAARARIAASDARKAIRSKSLLDGAGMPDKLKDCSAKDPERRELFIVEGDSAGGSAVRARDPETQAILPIRGKILNVERARIDKM 510
            TVN YAR++G LKEKDDNLTGEDIREGLTAIISARIG+PQFEGQTKSKLGNVSMRSLVERVTNERMA+WLEEHPGE K  +TKATNAARAR+AA+DARKAIRSKSLLDGAGMPDKLKDCS+KDP  RELFIVEGDSAGGSAVRARDP TQAILPIRGKILNVERARIDKM
Sbjct:  326 TVNTYARNKGLLKEKDDNLTGEDIREGLTAIISARIGEPQFEGQTKSKLGNVSMRSLVERVTNERMADWLEEHPGEAKATITKATNAARARVAAADARKAIRSKSLLDGAGMPDKLKDCSSKDPAERELFIVEGDSAGGSAVRARDPRTQAILPIRGKILNVERARIDKM 495
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BLAST of mRNA_M-pyrifera_M_contig126066.5266.1 vs. uniprot
Match: A0A3M2GUA3_ACTSX (DNA topoisomerase (ATP-hydrolyzing) n=1 Tax=Actinomyces sp. TaxID=29317 RepID=A0A3M2GUA3_ACTSX)

HSP 1 Score: 300 bits (769), Expect = 3.520e-96
Identity = 151/170 (88.82%), Postives = 164/170 (96.47%), Query Frame = 1 
Query:    1 TVNAYARDRGFLKEKDDNLTGEDIREGLTAIISARIGDPQFEGQTKSKLGNVSMRSLVERVTNERMAEWLEEHPGEGKVIVTKATNAARARIAASDARKAIRSKSLLDGAGMPDKLKDCSAKDPERRELFIVEGDSAGGSAVRARDPETQAILPIRGKILNVERARIDKM 510
            TVNAYARD+G LKE+DDNLTG+DIREGLTAIISAR+ +PQFEGQTKSKLGNVSMRSLVE+VTNER+AEWLEEHPGE + IVTKA NAARAR+AA+DARKAIR+KSLLDGAGMPDKLKDCSA++PERRELFIVEGDSAGGSAVRARDPETQAILPIRGKILNVERAR DKM
Sbjct:  318 TVNAYARDKGLLKERDDNLTGDDIREGLTAIISARLAEPQFEGQTKSKLGNVSMRSLVEKVTNERLAEWLEEHPGEARKIVTKALNAARARVAAADARKAIRAKSLLDGAGMPDKLKDCSAREPERRELFIVEGDSAGGSAVRARDPETQAILPIRGKILNVERARPDKM 487
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BLAST of mRNA_M-pyrifera_M_contig126066.5266.1 vs. uniprot
Match: A0A351IV20_9ACTN (DNA topoisomerase (ATP-hydrolyzing) n=5 Tax=root TaxID=1 RepID=A0A351IV20_9ACTN)

HSP 1 Score: 263 bits (671), Expect = 9.180e-82
Identity = 132/169 (78.11%), Postives = 144/169 (85.21%), Query Frame = 1 
Query:    4 VNAYARDRGFLKEKDDNLTGEDIREGLTAIISARIGDPQFEGQTKSKLGNVSMRSLVERVTNERMAEWLEEHPGEGKVIVTKATNAARARIAASDARKAIRSKSLLDGAGMPDKLKDCSAKDPERRELFIVEGDSAGGSAVRARDPETQAILPIRGKILNVERARIDKM 510
            VN YAR RG LKE DDNL GEDIREGLTAIIS R+ DPQFEGQTKSKLGNV  RSLV+R TNE++AEWLEEHP EGK I+ KA NA RAR+AA DARK+ R KS LDGAGMPDKLKDC+++DP   ELFIVEGDSAGGSAV+ARDP T AILPIRGKILNVERAR D+M
Sbjct:  302 VNRYARKRGLLKENDDNLQGEDIREGLTAIISVRLEDPQFEGQTKSKLGNVETRSLVQRTTNEKLAEWLEEHPSEGKRILQKAVNAQRARVAAQDARKSARRKSALDGAGMPDKLKDCASRDPRESELFIVEGDSAGGSAVQARDPRTMAILPIRGKILNVERARADRM 470
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BLAST of mRNA_M-pyrifera_M_contig126066.5266.1 vs. uniprot
Match: A0A800LRE1_9ACTN (DNA topoisomerase (ATP-hydrolyzing) n=1 Tax=Acidimicrobiia bacterium TaxID=2080302 RepID=A0A800LRE1_9ACTN)

HSP 1 Score: 261 bits (668), Expect = 2.500e-81
Identity = 131/169 (77.51%), Postives = 145/169 (85.80%), Query Frame = 1 
Query:    4 VNAYARDRGFLKEKDDNLTGEDIREGLTAIISARIGDPQFEGQTKSKLGNVSMRSLVERVTNERMAEWLEEHPGEGKVIVTKATNAARARIAASDARKAIRSKSLLDGAGMPDKLKDCSAKDPERRELFIVEGDSAGGSAVRARDPETQAILPIRGKILNVERARIDKM 510
            +N YAR +G LKEKDDNL GEDIREG+T IIS R+GDPQFEGQTKSKLGNV +RSLV++ TNER+AEWLEEHP E K ++ KA NA RARIAA DARK+ R KS LDGAGMPDKLKDCS++DP   ELFIVEGDSAGGSAV+ARDP T AILPIRGKILNVERAR DKM
Sbjct:  301 MNRYARVKGLLKEKDDNLQGEDIREGMTTIISVRLGDPQFEGQTKSKLGNVEIRSLVQKATNERLAEWLEEHPSEAKRVLQKAINAQRARIAAHDARKSARRKSALDGAGMPDKLKDCSSRDPRESELFIVEGDSAGGSAVQARDPRTMAILPIRGKILNVERARPDKM 469
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BLAST of mRNA_M-pyrifera_M_contig126066.5266.1 vs. uniprot
Match: A0A382KS51_9ZZZZ (DNA topoisomerase (ATP-hydrolyzing) (Fragment) n=1 Tax=marine metagenome TaxID=408172 RepID=A0A382KS51_9ZZZZ)

HSP 1 Score: 254 bits (648), Expect = 8.490e-81
Identity = 121/169 (71.60%), Postives = 147/169 (86.98%), Query Frame = 1 
Query:    4 VNAYARDRGFLKEKDDNLTGEDIREGLTAIISARIGDPQFEGQTKSKLGNVSMRSLVERVTNERMAEWLEEHPGEGKVIVTKATNAARARIAASDARKAIRSKSLLDGAGMPDKLKDCSAKDPERRELFIVEGDSAGGSAVRARDPETQAILPIRGKILNVERARIDKM 510
            +N +A+ RG +K+KDDNL GEDIREGLTAIIS R+G+PQFEGQTKSKLGNV +RSLV++ TNE++++WLEEHP E K+I+ KA NA RAR+AA DAR++ R KS LDGAGMP+KLKDCS++DP   ELFIVEGDSAGGSA++ARDP T AILP+RGKILNVERAR D+M
Sbjct:  121 MNKHAKKRGLIKDKDDNLQGEDIREGLTAIISVRLGEPQFEGQTKSKLGNVDIRSLVQKTTNEKLSDWLEEHPAEAKIILQKAINAQRARLAAQDARRSARRKSALDGAGMPEKLKDCSSRDPRESELFIVEGDSAGGSAIQARDPRTMAILPLRGKILNVERARADRM 289
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BLAST of mRNA_M-pyrifera_M_contig126066.5266.1 vs. uniprot
Match: A0A382FPE9_9ZZZZ (DNA topoisomerase (ATP-hydrolyzing) (Fragment) n=1 Tax=marine metagenome TaxID=408172 RepID=A0A382FPE9_9ZZZZ)

HSP 1 Score: 253 bits (645), Expect = 1.920e-80
Identity = 123/169 (72.78%), Postives = 147/169 (86.98%), Query Frame = 1 
Query:    4 VNAYARDRGFLKEKDDNLTGEDIREGLTAIISARIGDPQFEGQTKSKLGNVSMRSLVERVTNERMAEWLEEHPGEGKVIVTKATNAARARIAASDARKAIRSKSLLDGAGMPDKLKDCSAKDPERRELFIVEGDSAGGSAVRARDPETQAILPIRGKILNVERARIDKM 510
            VN YAR RG +K+KDDNL GEDIREGLTAI+S R+ DPQFEGQTKSKLGNV +RSLV++VTN+++A+WLEE+P E K +V KA NA RAR+AA +AR++ R KS LDGAGMPDKLKDC++KDP+  EL IVEGDSAGGSAV+ARDP T A+LPIRGKILNVERAR+D+M
Sbjct:   67 VNKYARRRGAIKDKDDNLQGEDIREGLTAIVSVRLVDPQFEGQTKSKLGNVEIRSLVQKVTNDKLADWLEENPSEAKRVVEKAVNAQRARLAAQEARRSARRKSALDGAGMPDKLKDCTSKDPKESELLIVEGDSAGGSAVQARDPRTMAVLPIRGKILNVERARVDRM 235
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BLAST of mRNA_M-pyrifera_M_contig126066.5266.1 vs. uniprot
Match: A0A3D5K493_9ACTN (DNA gyrase subunit B n=3 Tax=Acidimicrobiaceae bacterium TaxID=2024894 RepID=A0A3D5K493_9ACTN)

HSP 1 Score: 259 bits (661), Expect = 2.790e-80
Identity = 129/169 (76.33%), Postives = 143/169 (84.62%), Query Frame = 1 
Query:    4 VNAYARDRGFLKEKDDNLTGEDIREGLTAIISARIGDPQFEGQTKSKLGNVSMRSLVERVTNERMAEWLEEHPGEGKVIVTKATNAARARIAASDARKAIRSKSLLDGAGMPDKLKDCSAKDPERRELFIVEGDSAGGSAVRARDPETQAILPIRGKILNVERARIDKM 510
            +N YA+ R  LK+ DDNL GEDIREGLTAIIS R+ DPQFEGQTKSKLGNV MRSLV+R TN+R+A+WLEEHP E K IV KA NA RAR+AA DARK+ R KS LDGAGMPDKLKDC++KDP   ELFIVEGDSAGGSAV+ARDP T AILPIRGKILNVERAR D+M
Sbjct:  302 MNRYAKKRNLLKDNDDNLQGEDIREGLTAIISVRLSDPQFEGQTKSKLGNVEMRSLVQRTTNDRLADWLEEHPPEAKAIVQKAINAQRARVAAQDARKSARRKSALDGAGMPDKLKDCASKDPRESELFIVEGDSAGGSAVQARDPRTMAILPIRGKILNVERARADRM 470
[Close]
BLAST of mRNA_M-pyrifera_M_contig126066.5266.1 vs. uniprot
Match: A0A6I3A784_9ACTN (DNA topoisomerase (ATP-hydrolyzing) n=2 Tax=root TaxID=1 RepID=A0A6I3A784_9ACTN)

HSP 1 Score: 258 bits (658), Expect = 2.020e-79
Identity = 125/169 (73.96%), Postives = 146/169 (86.39%), Query Frame = 1 
Query:    4 VNAYARDRGFLKEKDDNLTGEDIREGLTAIISARIGDPQFEGQTKSKLGNVSMRSLVERVTNERMAEWLEEHPGEGKVIVTKATNAARARIAASDARKAIRSKSLLDGAGMPDKLKDCSAKDPERRELFIVEGDSAGGSAVRARDPETQAILPIRGKILNVERARIDKM 510
            +N YAR +G LKEKD+NL GEDIREGLTAII+ ++ +PQFEGQTK KLGNVS+RSLVER TN+++AEWLEEHP E +  V KAT +ARAR+AA +AR  +R K+LLDGAGMPDKL+DCS+ DP  RELFIVEGDSAGGSA +ARDP TQAILPIRGKILNVERAR+DKM
Sbjct:  348 INKYARAKGLLKEKDENLQGEDIREGLTAIIAVKLREPQFEGQTKGKLGNVSVRSLVERATNDKLAEWLEEHPSEARAAVAKATQSARARVAAQNARNTMRRKTLLDGAGMPDKLRDCSSSDPRERELFIVEGDSAGGSATQARDPRTQAILPIRGKILNVERARVDKM 516
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig126066.5266.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A2D8GI33_9ACTN1.550e-10092.94DNA topoisomerase (ATP-hydrolyzing) n=8 Tax=Acidim... [more]
A0A7X5WVW5_9ACTN8.980e-9991.18DNA topoisomerase (ATP-hydrolyzing) n=1 Tax=Actino... [more]
A0A7K1ZBL8_9ACTN9.960e-9890.59DNA topoisomerase (ATP-hydrolyzing) n=2 Tax=Acidim... [more]
A0A3M2GUA3_ACTSX3.520e-9688.82DNA topoisomerase (ATP-hydrolyzing) n=1 Tax=Actino... [more]
A0A351IV20_9ACTN9.180e-8278.11DNA topoisomerase (ATP-hydrolyzing) n=5 Tax=root T... [more]
A0A800LRE1_9ACTN2.500e-8177.51DNA topoisomerase (ATP-hydrolyzing) n=1 Tax=Acidim... [more]
A0A382KS51_9ZZZZ8.490e-8171.60DNA topoisomerase (ATP-hydrolyzing) (Fragment) n=1... [more]
A0A382FPE9_9ZZZZ1.920e-8072.78DNA topoisomerase (ATP-hydrolyzing) (Fragment) n=1... [more]
A0A3D5K493_9ACTN2.790e-8076.33DNA gyrase subunit B n=3 Tax=Acidimicrobiaceae bac... [more]
A0A6I3A784_9ACTN2.020e-7973.96DNA topoisomerase (ATP-hydrolyzing) n=2 Tax=root T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig126066contigM-pyrifera_M_contig126066:3..512 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score238.8
Seed ortholog evalue1.8e-60
Seed eggNOG ortholog1121877.JQKF01000017_gene188
Preferred namegyrB
KEGG koko:K02470
Hectar predicted targeting categoryother localisation
GOsGO:0000166,GO:0000287,GO:0003674,GO:0003824,GO:0003916,GO:0003918,GO:0005488,GO:0005524,GO:0005575,GO:0005618,GO:0005623,GO:0005886,GO:0006139,GO:0006259,GO:0006265,GO:0006725,GO:0006807,GO:0006996,GO:0007059,GO:0008094,GO:0008144,GO:0008150,GO:0008152,GO:0009295,GO:0009987,GO:0016020,GO:0016043,GO:0016462,GO:0016787,GO:0016817,GO:0016818,GO:0016853,GO:0016887,GO:0017076,GO:0017111,GO:0030312,GO:0030554,GO:0032553,GO:0032555,GO:0032559,GO:0034335,GO:0034641,GO:0035639,GO:0036094,GO:0040007,GO:0042623,GO:0043167,GO:0043168,GO:0043169,GO:0043170,GO:0044237,GO:0044238,GO:0044260,GO:0044464,GO:0046483,GO:0046872,GO:0051276,GO:0061505,GO:0071103,GO:0071704,GO:0071840,GO:0071944,GO:0090304,GO:0097159,GO:0097367,GO:0140097,GO:1901265,GO:1901360,GO:1901363
EggNOG free text desc.TopoisomeraseII
EggNOG OGs2GKGP@201174,4CMQZ@84992,COG0187@1,COG0187@2
EC5.99.1.3
COG Functional cat.L
Best tax levelAcidimicrobiia
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko03032,ko03400
Exons1
Model size510
Cds size510
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815775.9699297-CDS-M-pyrifera_M_contig126066:2..5121622815775.9699297-CDS-M-pyrifera_M_contig126066:2..512Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig126066 3..512 +
1692277563.961744-CDS-M-pyrifera_M_contig126066:2..5121692277563.961744-CDS-M-pyrifera_M_contig126066:2..512Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig126066 3..512 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig126066.5266.1prot_M-pyrifera_M_contig126066.5266.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig126066 3..512 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig126066.5266.1

>prot_M-pyrifera_M_contig126066.5266.1 ID=prot_M-pyrifera_M_contig126066.5266.1|Name=mRNA_M-pyrifera_M_contig126066.5266.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=170bp
TVNAYARDRGFLKEKDDNLTGEDIREGLTAIISARIGDPQFEGQTKSKLG
NVSMRSLVERVTNERMAEWLEEHPGEGKVIVTKATNAARARIAASDARKA
IRSKSLLDGAGMPDKLKDCSAKDPERRELFIVEGDSAGGSAVRARDPETQ
AILPIRGKILNVERARIDKM
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mRNA from alignment at M-pyrifera_M_contig126066:3..512+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig126066.5266.1 ID=mRNA_M-pyrifera_M_contig126066.5266.1|Name=mRNA_M-pyrifera_M_contig126066.5266.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=510bp|location=Sequence derived from alignment at M-pyrifera_M_contig126066:3..512+ (Macrocystis pyrifera P11B4 male)
ACGGTCAACGCCTATGCCCGTGATCGCGGCTTCCTCAAGGAGAAGGACGA
CAACCTCACCGGCGAAGACATCCGCGAGGGTCTGACCGCGATCATCAGCG
CCCGCATCGGCGACCCCCAGTTCGAGGGTCAGACCAAGTCGAAGCTCGGC
AACGTCTCGATGCGATCCCTGGTCGAGCGGGTCACCAACGAGCGCATGGC
CGAGTGGCTCGAAGAACACCCCGGCGAAGGCAAGGTCATCGTCACCAAGG
CCACCAACGCCGCCCGGGCCCGCATCGCGGCGTCCGACGCCCGCAAGGCG
ATCCGGTCGAAGTCGTTGCTCGACGGCGCCGGCATGCCCGACAAGTTGAA
GGACTGTTCGGCCAAGGATCCCGAGCGACGCGAACTCTTCATCGTCGAGG
GCGATTCTGCCGGCGGTTCGGCGGTGCGGGCCCGAGATCCCGAGACCCAG
GCCATCCTGCCGATCCGCGGCAAGATCCTCAATGTCGAGCGAGCTCGCAT
CGACAAGATG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig126066:3..512+

>mRNA_M-pyrifera_M_contig126066.5266.1 ID=mRNA_M-pyrifera_M_contig126066.5266.1|Name=mRNA_M-pyrifera_M_contig126066.5266.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1020bp|location=Sequence derived from alignment at M-pyrifera_M_contig126066:3..512+ (Macrocystis pyrifera P11B4 male)
ACGGTCAACGCCTATGCCCGTGATCGCGGCTTCCTCAAGGAGAAGGACGA
CAACCTCACCGGCGAAGACATCCGCGAGGGTCTGACCGCGATCATCAGCG
CCCGCATCGGCGACCCCCAGTTCGAGGGTCAGACCAAGTCGAAGCTCGGC
AACGTCTCGATGCGATCCCTGGTCGAGCGGGTCACCAACGAGCGCATGGC
CGAGTGGCTCGAAGAACACCCCGGCGAAGGCAAGGTCATCGTCACCAAGG
CCACCAACGCCGCCCGGGCCCGCATCGCGGCGTCCGACGCCCGCAAGGCG
ATCCGGTCGAAGTCGTTGCTCGACGGCGCCGGCATGCCCGACAAGTTGAA
GGACTGTTCGGCCAAGGATCCCGAGCGACGCGAACTCTTCATCGTCGAGG
GCGATTCTGCCGGCGGTTCGGCGGTGCGGGCCCGAGATCCCGAGACCCAG
GCCATCCTGCCGATCCGCGGCAAGATCCTCAATGTCGAGCGAGCTCGCAT
CGACAAGATGACGGTCAACGCCTATGCCCGTGATCGCGGCTTCCTCAAGG
AGAAGGACGACAACCTCACCGGCGAAGACATCCGCGAGGGTCTGACCGCG
ATCATCAGCGCCCGCATCGGCGACCCCCAGTTCGAGGGTCAGACCAAGTC
GAAGCTCGGCAACGTCTCGATGCGATCCCTGGTCGAGCGGGTCACCAACG
AGCGCATGGCCGAGTGGCTCGAAGAACACCCCGGCGAAGGCAAGGTCATC
GTCACCAAGGCCACCAACGCCGCCCGGGCCCGCATCGCGGCGTCCGACGC
CCGCAAGGCGATCCGGTCGAAGTCGTTGCTCGACGGCGCCGGCATGCCCG
ACAAGTTGAAGGACTGTTCGGCCAAGGATCCCGAGCGACGCGAACTCTTC
ATCGTCGAGGGCGATTCTGCCGGCGGTTCGGCGGTGCGGGCCCGAGATCC
CGAGACCCAGGCCATCCTGCCGATCCGCGGCAAGATCCTCAATGTCGAGC
GAGCTCGCATCGACAAGATG
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