mRNA_M-pyrifera_M_contig8575.19999.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig8575.19999.1
Unique NamemRNA_M-pyrifera_M_contig8575.19999.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig8575.19999.1 vs. uniprot
Match: D8LS34_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LS34_ECTSI)

HSP 1 Score: 137 bits (346), Expect = 2.440e-38
Identity = 64/74 (86.49%), Postives = 69/74 (93.24%), Query Frame = 1
Query:    4 QAFFGLNPGVTVLIEFDHVDKRPTKPAAPGDKRASETLPIFEGSEAVSGTATVLVAPAKKVDHLGIKAELIGQI 225
            QA FG NPGVT+L+EFDH+DKRPTKP+ PGD+RASE LPIFEGSEAVSGTATVLVAP KKVDHLGIKAELIGQI
Sbjct:    2 QALFGFNPGVTILVEFDHIDKRPTKPSTPGDRRASEALPIFEGSEAVSGTATVLVAPGKKVDHLGIKAELIGQI 75          
BLAST of mRNA_M-pyrifera_M_contig8575.19999.1 vs. uniprot
Match: A0A836CB39_9STRA (Vacuolar protein sorting-associated protein 26-domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CB39_9STRA)

HSP 1 Score: 104 bits (259), Expect = 2.150e-25
Identity = 48/73 (65.75%), Postives = 58/73 (79.45%), Query Frame = 1
Query:    7 AFFGLNPGVTVLIEFDHVDKRPTKPAAPGDKRASETLPIFEGSEAVSGTATVLVAPAKKVDHLGIKAELIGQI 225
            A+FGL PG TVL+EFD  ++RP++   PGDK+A E +PIF GSE VSG  TVLVAP KKVDH+GIK E+IGQI
Sbjct:    2 AWFGLQPGATVLLEFDGAERRPSRAPPPGDKKALERVPIFVGSEPVSGAVTVLVAPGKKVDHMGIKVEMIGQI 74          
BLAST of mRNA_M-pyrifera_M_contig8575.19999.1 vs. uniprot
Match: A0A7S2UYV3_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2UYV3_9STRA)

HSP 1 Score: 60.8 bits (146), Expect = 4.090e-9
Identity = 29/73 (39.73%), Postives = 42/73 (57.53%), Query Frame = 1
Query:    7 AFFGLNPGVTVLIEFDHVDKRPTKPAAPGDKRASETLPIFEGSEAVSGTATVLVAPAKKVDHLGIKAELIGQI 225
            + FG  P   + ++F     R  K      ++ S+ L IF G EAVSGT  V++ P K+++H GIK E+IGQI
Sbjct:    2 SLFGFGPAAEIRLDFADSATRTRKAVGAVGQKDSKQLLIFHGEEAVSGTVDVVLPPGKRLEHQGIKVEMIGQI 74          
BLAST of mRNA_M-pyrifera_M_contig8575.19999.1 vs. uniprot
Match: A0A7S1CEN8_9STRA (Hypothetical protein n=1 Tax=Bicosoecida sp. CB-2014 TaxID=1486930 RepID=A0A7S1CEN8_9STRA)

HSP 1 Score: 58.9 bits (141), Expect = 2.000e-8
Identity = 27/73 (36.99%), Postives = 43/73 (58.90%), Query Frame = 1
Query:    7 AFFGLNPGVTVLIEFDHVDKRPTKPAAPGDKRASETLPIFEGSEAVSGTATVLVAPAKKVDHLGIKAELIGQI 225
            + FG  P  T+ IE +  +KR  +     D      +P+F+ +E VSG  T++V  +KK++H+GIK EL+G I
Sbjct:    2 SIFGFGPSATIDIELNGAEKRAMRRQETLDGTEGVDVPVFQDTEDVSGKVTLVVPGSKKLEHIGIKVELLGHI 74          
BLAST of mRNA_M-pyrifera_M_contig8575.19999.1 vs. uniprot
Match: A0A5J4XEX0_9CHLO (Subunit of retromer complex n=1 Tax=Trebouxia sp. A1-2 TaxID=2608996 RepID=A0A5J4XEX0_9CHLO)

HSP 1 Score: 57.8 bits (138), Expect = 5.740e-8
Identity = 31/79 (39.24%), Postives = 44/79 (55.70%), Query Frame = 1
Query:    1 QQAFFG---LNPGVTVLIEFDHVDKRPTKPAA-PGDKRASETLPIFEGSEAVSGTATVLVAPAKKVDHLGIKAELIGQI 225
            Q+  FG   L    TV +EF   D++P K A+  G    +E LP++   ++V G   V   P KKV+H GIK +L+GQI
Sbjct:    4 QKGLFGGLTLGTNATVQLEFRRADRKPVKAASIKGKGSETEQLPLYTNKDSVVGEIRVANTPGKKVEHQGIKVQLLGQI 82          
BLAST of mRNA_M-pyrifera_M_contig8575.19999.1 vs. uniprot
Match: A0A8J2AHK6_9DINO (Hypothetical protein n=2 Tax=unclassified Amoebophrya TaxID=2558461 RepID=A0A8J2AHK6_9DINO)

HSP 1 Score: 56.6 bits (135), Expect = 1.370e-7
Identity = 34/73 (46.58%), Postives = 40/73 (54.79%), Query Frame = 1
Query:    7 AFFGLNPGVTVLIEFDHVDKRPTKPAAPGDKRASETLPIFEGSEAVSGTATVLVAPAKKVDHLGIKAELIGQI 225
             FFG  P  TV I  D  + R T P   G  +  E   I+   E V+GTA V V P KKVDH GI+ ELIGQ+
Sbjct:    4 GFFGGTP-CTVDITLDAEENRKTAPL--GRDKKGERAYIYTDGEDVNGTAVVSVRPGKKVDHQGIRVELIGQV 73          
BLAST of mRNA_M-pyrifera_M_contig8575.19999.1 vs. uniprot
Match: I0Z698_COCSC (Vacuolar protein sorting-associated protein 26 n=2 Tax=Coccomyxa TaxID=41891 RepID=I0Z698_COCSC)

HSP 1 Score: 56.6 bits (135), Expect = 1.480e-7
Identity = 32/73 (43.84%), Postives = 43/73 (58.90%), Query Frame = 1
Query:   16 GLNPGVTVLIEFDHVDKRPTKPA---APGDKRASETLPIFEGSEAVSGTATVLVAPAKKVDHLGIKAELIGQI 225
            GL  G TV +EF + D  P K A   A G +  +ETLP+F   + ++G   V   P KK++H GIK +LIGQI
Sbjct:    9 GLGQGGTVQLEFKNDDGAPVKTATVKAGGGE--TETLPLFTNKDTITGEVKVANIPGKKLEHQGIKVQLIGQI 79          
BLAST of mRNA_M-pyrifera_M_contig8575.19999.1 vs. uniprot
Match: A0A7S0L8Y1_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Coccolithus braarudii TaxID=221442 RepID=A0A7S0L8Y1_9EUKA)

HSP 1 Score: 55.1 bits (131), Expect = 1.680e-7
Identity = 27/63 (42.86%), Postives = 39/63 (61.90%), Query Frame = 1
Query:   37 VLIEFDHVDKRPTKPAAPGDKRASETLPIFEGSEAVSGTATVLVAPAKKVDHLGIKAELIGQI 225
            ++I+FD+ D      +   + + SE L +F+G + V GT  V + P KK+DH GIK ELIGQI
Sbjct:    1 IVIKFDNEDSMQMV-SMKNEHKDSEQLCLFQGKDTVGGTVEVNIKPGKKIDHTGIKIELIGQI 62          
BLAST of mRNA_M-pyrifera_M_contig8575.19999.1 vs. uniprot
Match: A0A0G4EAR0_VITBC (Uncharacterized protein n=1 Tax=Vitrella brassicaformis (strain CCMP3155) TaxID=1169540 RepID=A0A0G4EAR0_VITBC)

HSP 1 Score: 55.8 bits (133), Expect = 2.580e-7
Identity = 28/66 (42.42%), Postives = 39/66 (59.09%), Query Frame = 1
Query:   28 GVTVLIEFDHVDKRPTKPAAPGDKRASETLPIFEGSEAVSGTATVLVAPAKKVDHLGIKAELIGQI 225
            G ++ ++FDH  +R   P   G  R  E   I+   E VSG+A V + P KK+DH G+K ELIGQ+
Sbjct:   11 GCSLEVQFDHEPQRKMAPL--GRDRKGEKALIYSDGEDVSGSAMVSMKPGKKIDHQGVKIELIGQV 74          
BLAST of mRNA_M-pyrifera_M_contig8575.19999.1 vs. uniprot
Match: A0A7S2C2A7_9STRA (Hypothetical protein n=2 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2C2A7_9STRA)

HSP 1 Score: 55.1 bits (131), Expect = 4.910e-7
Identity = 28/70 (40.00%), Postives = 37/70 (52.86%), Query Frame = 1
Query:   13 FGLNPGVTVLIEFDHVDKRPTKPAAPGDKRASETLPIFEGSEAVSGTATVLVAPAKKVDHLGIKAELIGQ 222
            FG      + I FD    RPTK    G       LPIF+  E  +G+  V V P KK++H+GIK E++GQ
Sbjct:    7 FGSKSSDRIAISFDGGANRPTKRTGTGPTEV--VLPIFDNKEPFTGSVEVRVDPGKKLEHMGIKIEMVGQ 74          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig8575.19999.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LS34_ECTSI2.440e-3886.49Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A836CB39_9STRA2.150e-2565.75Vacuolar protein sorting-associated protein 26-dom... [more]
A0A7S2UYV3_9STRA4.090e-939.73Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
A0A7S1CEN8_9STRA2.000e-836.99Hypothetical protein n=1 Tax=Bicosoecida sp. CB-20... [more]
A0A5J4XEX0_9CHLO5.740e-839.24Subunit of retromer complex n=1 Tax=Trebouxia sp. ... [more]
A0A8J2AHK6_9DINO1.370e-746.58Hypothetical protein n=2 Tax=unclassified Amoeboph... [more]
I0Z698_COCSC1.480e-743.84Vacuolar protein sorting-associated protein 26 n=2... [more]
A0A7S0L8Y1_9EUKA1.680e-742.86Hypothetical protein (Fragment) n=1 Tax=Coccolithu... [more]
A0A0G4EAR0_VITBC2.580e-742.42Uncharacterized protein n=1 Tax=Vitrella brassicaf... [more]
A0A7S2C2A7_9STRA4.910e-740.00Hypothetical protein n=2 Tax=Dictyocha speculum Ta... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig8575contigM-pyrifera_M_contig8575:8564..9544 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score138.3
Seed ortholog evalue1.6e-30
Seed eggNOG ortholog2880.D8LS34
Preferred nameVPS26B
KEGG rclassRC00055,RC00523
KEGG koko:K01876,ko:K18466
KEGG ReactionR05577
KEGG Pathwayko00970,ko04144,map00970,map04144
KEGG ModuleM00359,M00360
Hectar predicted targeting categoryother localisation
GOsGO:0000322,GO:0000323,GO:0000324,GO:0000329,GO:0002376,GO:0003674,GO:0003824,GO:0005215,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005764,GO:0005768,GO:0005769,GO:0005770,GO:0005773,GO:0005774,GO:0005829,GO:0006810,GO:0006886,GO:0006950,GO:0006952,GO:0006955,GO:0007154,GO:0007165,GO:0007166,GO:0007267,GO:0008104,GO:0008150,GO:0008565,GO:0009894,GO:0009966,GO:0009987,GO:0010008,GO:0010033,GO:0010506,GO:0010646,GO:0012505,GO:0015031,GO:0015833,GO:0016020,GO:0016055,GO:0016192,GO:0016197,GO:0016241,GO:0016482,GO:0016787,GO:0019222,GO:0023051,GO:0023052,GO:0030111,GO:0030139,GO:0030904,GO:0030906,GO:0031090,GO:0031323,GO:0031329,GO:0031410,GO:0031982,GO:0032507,GO:0032991,GO:0033036,GO:0033365,GO:0034067,GO:0034097,GO:0034341,GO:0034613,GO:0042147,GO:0042221,GO:0042886,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044422,GO:0044424,GO:0044425,GO:0044433,GO:0044437,GO:0044440,GO:0044444,GO:0044446,GO:0044464,GO:0045053,GO:0045087,GO:0045184,GO:0045185,GO:0045335,GO:0046907,GO:0048583,GO:0050789,GO:0050794,GO:0050896,GO:0051179,GO:0051234,GO:0051235,GO:0051641,GO:0051649,GO:0051651,GO:0051716,GO:0065007,GO:0065008,GO:0070727,GO:0070887,GO:0071310,GO:0071345,GO:0071346,GO:0071702,GO:0071705,GO:0097422,GO:0097708,GO:0098588,GO:0098796,GO:0098805,GO:0098852,GO:0098876,GO:0198738,GO:1905114,GO:1990126
EggNOG free text desc.retrograde transport, endosome to Golgi
EggNOG OGsKOG3063@1,KOG3063@2759
Ec32 ortholog descriptionVacuolar protein sorting protein 26 related
Ec32 orthologEc-08_001990.1
EC6.1.1.12
COG Functional cat.E
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko01007,ko03016,ko03029,ko04131
Exons2
Model size234
Cds size234
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816767.9166982-CDS-M-pyrifera_M_contig8575:8563..86591622816767.9166982-CDS-M-pyrifera_M_contig8575:8563..8659Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8575 8564..8659 -
1692278166.7516165-CDS-M-pyrifera_M_contig8575:8563..86591692278166.7516165-CDS-M-pyrifera_M_contig8575:8563..8659Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8575 8564..8659 -
1622816767.9308057-CDS-M-pyrifera_M_contig8575:9406..95441622816767.9308057-CDS-M-pyrifera_M_contig8575:9406..9544Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8575 9407..9544 -
1692278166.7653277-CDS-M-pyrifera_M_contig8575:9406..95441692278166.7653277-CDS-M-pyrifera_M_contig8575:9406..9544Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8575 9407..9544 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig8575.19999.1prot_M-pyrifera_M_contig8575.19999.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig8575 8564..9544 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig8575.19999.1

>prot_M-pyrifera_M_contig8575.19999.1 ID=prot_M-pyrifera_M_contig8575.19999.1|Name=mRNA_M-pyrifera_M_contig8575.19999.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=78bp
QQAFFGLNPGVTVLIEFDHVDKRPTKPAAPGDKRASETLPIFEGSEAVSG
TATVLVAPAKKVDHLGIKAELIGQIGE*
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mRNA from alignment at M-pyrifera_M_contig8575:8564..9544-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig8575.19999.1 ID=mRNA_M-pyrifera_M_contig8575.19999.1|Name=mRNA_M-pyrifera_M_contig8575.19999.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=981bp|location=Sequence derived from alignment at M-pyrifera_M_contig8575:8564..9544- (Macrocystis pyrifera P11B4 male)
CAGCAAGCTTTCTTCGGGCTCAACCCGGGGGTCACGGTCCTGATCGAGTT CGACCACGTGGATAAGAGACCCACCAAGCCCGCCGCCCCGGGAGACAAGC GGGCTTCGGAGACCCTCCCTATCTTCGAGGGGTCCGAGGTGCGCATGGTT TGTGGGCGGGGATTCGATTTGTTTGTTGTTGTGTGGTTGTTTTTGTTTTG TCGTAGTAGTAGTTGTAGTTGTGGTTGCTGTTGTTATTTGTGAAATTTTA CACAGTGCAGCAACGACCCCTCTTGCCTGGCCGGGTAGCGTAAGGGTCGA TGCCCTTTGAGGTTGATTATGGCCCCGATTCGGGCACATATCATTTGGTT CTGTCGGTGCTGCCGTCTCAAGCTATGCTGCGATATATAACGGGCTGATC GCGTTTGTCTGAACAGCGAGTTAGGTTTTGTTATTCCGGCGGCGACCACT GCTGTGACGGCGGCTGCTGTACTCATGACTTTTGGACGACGGGTGCTCTA GGATGACTGACTGCTGTGTGATGACTGCCGTAAGCACAGGTGTTTCGTGA TGTGTGATGATTTATTTATTTTTTCCATCCCTTTTATTATCTTCGCTCTT ACTGCTCCTGCTCCCTCCTAGTCGTTAAGTCAAATCAGGGGTCACAGCAG TAGGGTTTTATCTTCCCTCCCCCTACGGCACGTTCCTTGCAGTTTTTGTC ACGAGAAGGGTTCAACGCTTTCTTCCCTCGTCGACTCGCGTAGAATTGCG CCTACCCACTCTATCGTAGGCGCTGTCGGCAGTGCTCGAGGCTAACCGCT ACCGTAGACCATCGGGGTGACCGACTGCTCTATAATGACTGCTGTAACTG TTGACTGCCGTGTTTTTTCCCCGGCTCATTTTCAGGCGGTGAGCGGAACA GCGACGGTTCTCGTTGCGCCGGCGAAAAAGGTGGACCACCTGGGGATCAA GGCCGAACTCATCGGCCAGATAGGCGAGTAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig8575:8564..9544-

>mRNA_M-pyrifera_M_contig8575.19999.1 ID=mRNA_M-pyrifera_M_contig8575.19999.1|Name=mRNA_M-pyrifera_M_contig8575.19999.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=468bp|location=Sequence derived from alignment at M-pyrifera_M_contig8575:8564..9544- (Macrocystis pyrifera P11B4 male)
CAGCAAGCTTTCTTCGGGCTCAACCCGGGGGTCACGGTCCTGATCGAGTT
CGACCACGTGGATAAGAGACCCACCAAGCCCGCCGCCCCGGGAGACAAGC
GGGCTTCGGAGACCCTCCCTATCTTCGAGGGGTCCGAGCAGCAAGCTTTC
TTCGGGCTCAACCCGGGGGTCACGGTCCTGATCGAGTTCGACCACGTGGA
TAAGAGACCCACCAAGCCCGCCGCCCCGGGAGACAAGCGGGCTTCGGAGA
CCCTCCCTATCTTCGAGGGGTCCGAGGCGGTGAGCGGAACAGCGACGGTT
CTCGTTGCGCCGGCGAAAAAGGTGGACCACCTGGGGATCAAGGCCGAACT
CATCGGCCAGATAGGCGAGTAGGCGGTGAGCGGAACAGCGACGGTTCTCG
TTGCGCCGGCGAAAAAGGTGGACCACCTGGGGATCAAGGCCGAACTCATC
GGCCAGATAGGCGAGTAG
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