mRNA_M-pyrifera_M_contig84623.19723.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig84623.19723.1
Unique NamemRNA_M-pyrifera_M_contig84623.19723.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig84623.19723.1 vs. uniprot
Match: A0A8C9TCL4_SCLFO (DEP domain containing 5, GATOR1 subcomplex subunit n=1 Tax=Scleropages formosus TaxID=113540 RepID=A0A8C9TCL4_SCLFO)

HSP 1 Score: 64.3 bits (155), Expect = 4.900e-9
Identity = 45/147 (30.61%), Postives = 78/147 (53.06%), Query Frame = 1
Query:   34 DLVLSASLFPASAVGKVAQLRQKRAPNAPALGFLTVQAVRQLPSNAAASVAKGVAEVLGFASRMAVEVELVEDQVSREAAALSSVVLLFKGQYIGRADMYHVKRSLVGECIQRSKEVLYGSISAEVHALHRDGEVRSSGLVTEDTRV 474
            +L+++  +FP  ++G + ++       +P L  L V+++++       SV + VA+     +   V V +V+ +       L  V L FK QYIGR DM+ +K+SLVG C   +++V +  I A+   L   GE  + G ++EDTRV
Sbjct:   22 ELMVNPKVFPQVSIGDIVEIAHPNDEYSPLL--LQVRSLKEDLQKETISVDQTVAQAFKLRAYQDVYVNIVDPK----DVTLDLVELTFKDQYIGRGDMWRLKKSLVGTCAYVTQKVEFAGIRAQATELWVKGEKVTCGYISEDTRV 162          
BLAST of mRNA_M-pyrifera_M_contig84623.19723.1 vs. uniprot
Match: UPI0008781A92 (GATOR complex protein DEPDC5 isoform X6 n=2 Tax=Scleropages formosus TaxID=113540 RepID=UPI0008781A92)

HSP 1 Score: 64.3 bits (155), Expect = 5.030e-9
Identity = 45/147 (30.61%), Postives = 78/147 (53.06%), Query Frame = 1
Query:   34 DLVLSASLFPASAVGKVAQLRQKRAPNAPALGFLTVQAVRQLPSNAAASVAKGVAEVLGFASRMAVEVELVEDQVSREAAALSSVVLLFKGQYIGRADMYHVKRSLVGECIQRSKEVLYGSISAEVHALHRDGEVRSSGLVTEDTRV 474
            +L+++  +FP  ++G + ++       +P L  L V+++++       SV + VA+     +   V V +V+ +       L  V L FK QYIGR DM+ +K+SLVG C   +++V +  I A+   L   GE  + G ++EDTRV
Sbjct:   72 ELMVNPKVFPQVSIGDIVEIAHPNDEYSPLL--LQVRSLKEDLQKETISVDQTVAQAFKLRAYQDVYVNIVDPK----DVTLDLVELTFKDQYIGRGDMWRLKKSLVGTCAYVTQKVEFAGIRAQATELWVKGEKVTCGYISEDTRV 212          
BLAST of mRNA_M-pyrifera_M_contig84623.19723.1 vs. uniprot
Match: A0A8C9T722_SCLFO (DEP domain containing 5, GATOR1 subcomplex subunit n=1 Tax=Scleropages formosus TaxID=113540 RepID=A0A8C9T722_SCLFO)

HSP 1 Score: 64.3 bits (155), Expect = 5.120e-9
Identity = 45/147 (30.61%), Postives = 78/147 (53.06%), Query Frame = 1
Query:   34 DLVLSASLFPASAVGKVAQLRQKRAPNAPALGFLTVQAVRQLPSNAAASVAKGVAEVLGFASRMAVEVELVEDQVSREAAALSSVVLLFKGQYIGRADMYHVKRSLVGECIQRSKEVLYGSISAEVHALHRDGEVRSSGLVTEDTRV 474
            +L+++  +FP  ++G + ++       +P L  L V+++++       SV + VA+     +   V V +V+ +       L  V L FK QYIGR DM+ +K+SLVG C   +++V +  I A+   L   GE  + G ++EDTRV
Sbjct:   22 ELMVNPKVFPQVSIGDIVEIAHPNDEYSPLL--LQVRSLKEDLQKETISVDQTVAQAFKLRAYQDVYVNIVDPK----DVTLDLVELTFKDQYIGRGDMWRLKKSLVGTCAYVTQKVEFAGIRAQATELWVKGEKVTCGYISEDTRV 162          
BLAST of mRNA_M-pyrifera_M_contig84623.19723.1 vs. uniprot
Match: A0A8C9S1F0_SCLFO (DEP domain containing 5, GATOR1 subcomplex subunit n=2 Tax=Scleropages formosus TaxID=113540 RepID=A0A8C9S1F0_SCLFO)

HSP 1 Score: 64.3 bits (155), Expect = 5.130e-9
Identity = 45/147 (30.61%), Postives = 78/147 (53.06%), Query Frame = 1
Query:   34 DLVLSASLFPASAVGKVAQLRQKRAPNAPALGFLTVQAVRQLPSNAAASVAKGVAEVLGFASRMAVEVELVEDQVSREAAALSSVVLLFKGQYIGRADMYHVKRSLVGECIQRSKEVLYGSISAEVHALHRDGEVRSSGLVTEDTRV 474
            +L+++  +FP  ++G + ++       +P L  L V+++++       SV + VA+     +   V V +V+ +       L  V L FK QYIGR DM+ +K+SLVG C   +++V +  I A+   L   GE  + G ++EDTRV
Sbjct:   22 ELMVNPKVFPQVSIGDIVEIAHPNDEYSPLL--LQVRSLKEDLQKETISVDQTVAQAFKLRAYQDVYVNIVDPK----DVTLDLVELTFKDQYIGRGDMWRLKKSLVGTCAYVTQKVEFAGIRAQATELWVKGEKVTCGYISEDTRV 162          
BLAST of mRNA_M-pyrifera_M_contig84623.19723.1 vs. uniprot
Match: A0A8C9WQB8_SCLFO (DEP domain containing 5, GATOR1 subcomplex subunit n=3 Tax=Scleropages formosus TaxID=113540 RepID=A0A8C9WQB8_SCLFO)

HSP 1 Score: 64.3 bits (155), Expect = 5.140e-9
Identity = 45/147 (30.61%), Postives = 78/147 (53.06%), Query Frame = 1
Query:   34 DLVLSASLFPASAVGKVAQLRQKRAPNAPALGFLTVQAVRQLPSNAAASVAKGVAEVLGFASRMAVEVELVEDQVSREAAALSSVVLLFKGQYIGRADMYHVKRSLVGECIQRSKEVLYGSISAEVHALHRDGEVRSSGLVTEDTRV 474
            +L+++  +FP  ++G + ++       +P L  L V+++++       SV + VA+     +   V V +V+ +       L  V L FK QYIGR DM+ +K+SLVG C   +++V +  I A+   L   GE  + G ++EDTRV
Sbjct:   22 ELMVNPKVFPQVSIGDIVEIAHPNDEYSPLL--LQVRSLKEDLQKETISVDQTVAQAFKLRAYQDVYVNIVDPK----DVTLDLVELTFKDQYIGRGDMWRLKKSLVGTCAYVTQKVEFAGIRAQATELWVKGEKVTCGYISEDTRV 162          
BLAST of mRNA_M-pyrifera_M_contig84623.19723.1 vs. uniprot
Match: A0A8C9RXC2_SCLFO (DEP domain containing 5, GATOR1 subcomplex subunit n=1 Tax=Scleropages formosus TaxID=113540 RepID=A0A8C9RXC2_SCLFO)

HSP 1 Score: 64.3 bits (155), Expect = 5.150e-9
Identity = 45/147 (30.61%), Postives = 78/147 (53.06%), Query Frame = 1
Query:   34 DLVLSASLFPASAVGKVAQLRQKRAPNAPALGFLTVQAVRQLPSNAAASVAKGVAEVLGFASRMAVEVELVEDQVSREAAALSSVVLLFKGQYIGRADMYHVKRSLVGECIQRSKEVLYGSISAEVHALHRDGEVRSSGLVTEDTRV 474
            +L+++  +FP  ++G + ++       +P L  L V+++++       SV + VA+     +   V V +V+ +       L  V L FK QYIGR DM+ +K+SLVG C   +++V +  I A+   L   GE  + G ++EDTRV
Sbjct:   35 ELMVNPKVFPQVSIGDIVEIAHPNDEYSPLL--LQVRSLKEDLQKETISVDQTVAQAFKLRAYQDVYVNIVDPK----DVTLDLVELTFKDQYIGRGDMWRLKKSLVGTCAYVTQKVEFAGIRAQATELWVKGEKVTCGYISEDTRV 175          
BLAST of mRNA_M-pyrifera_M_contig84623.19723.1 vs. uniprot
Match: A0A8C9S679_SCLFO (DEP domain containing 5, GATOR1 subcomplex subunit n=1 Tax=Scleropages formosus TaxID=113540 RepID=A0A8C9S679_SCLFO)

HSP 1 Score: 64.3 bits (155), Expect = 5.160e-9
Identity = 45/147 (30.61%), Postives = 78/147 (53.06%), Query Frame = 1
Query:   34 DLVLSASLFPASAVGKVAQLRQKRAPNAPALGFLTVQAVRQLPSNAAASVAKGVAEVLGFASRMAVEVELVEDQVSREAAALSSVVLLFKGQYIGRADMYHVKRSLVGECIQRSKEVLYGSISAEVHALHRDGEVRSSGLVTEDTRV 474
            +L+++  +FP  ++G + ++       +P L  L V+++++       SV + VA+     +   V V +V+ +       L  V L FK QYIGR DM+ +K+SLVG C   +++V +  I A+   L   GE  + G ++EDTRV
Sbjct:   35 ELMVNPKVFPQVSIGDIVEIAHPNDEYSPLL--LQVRSLKEDLQKETISVDQTVAQAFKLRAYQDVYVNIVDPK----DVTLDLVELTFKDQYIGRGDMWRLKKSLVGTCAYVTQKVEFAGIRAQATELWVKGEKVTCGYISEDTRV 175          
BLAST of mRNA_M-pyrifera_M_contig84623.19723.1 vs. uniprot
Match: UPI000A1C7213 (DEP domain-containing protein 5 n=1 Tax=Boleophthalmus pectinirostris TaxID=150288 RepID=UPI000A1C7213)

HSP 1 Score: 63.9 bits (154), Expect = 7.000e-9
Identity = 46/147 (31.29%), Postives = 79/147 (53.74%), Query Frame = 1
Query:   34 DLVLSASLFPASAVGKVAQLRQKRAPNAPALGFLTVQAVRQLPSNAAASVAKGVAEVLGFASRMAVEVELVEDQVSREAAALSSVVLLFKGQYIGRADMYHVKRSLVGECIQRSKEVLYGSISAEVHALHRDGEVRSSGLVTEDTRV 474
            +LV++  +FP +++G + ++       +P L  L V+++++       SV + VA+V    +   V V +V+ +       L  V L FK QYIGR DM+ +K+SLV  C   +++V +  I A+   L   GE  + G ++EDTRV
Sbjct:   22 ELVVNPKVFPQASLGDLVEIAHPTDEYSPLL--LQVKSLKEDLQKETISVDQTVAQVFKLRAYQDVIVNIVDPK----DVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYVTQKVEFAGIRAQASELWVKGEKVTCGYISEDTRV 162          
BLAST of mRNA_M-pyrifera_M_contig84623.19723.1 vs. uniprot
Match: UPI0004954B71 (GATOR complex protein DEPDC5 isoform X1 n=3 Tax=Cynoglossus semilaevis TaxID=244447 RepID=UPI0004954B71)

HSP 1 Score: 63.9 bits (154), Expect = 7.000e-9
Identity = 46/147 (31.29%), Postives = 79/147 (53.74%), Query Frame = 1
Query:   34 DLVLSASLFPASAVGKVAQLRQKRAPNAPALGFLTVQAVRQLPSNAAASVAKGVAEVLGFASRMAVEVELVEDQVSREAAALSSVVLLFKGQYIGRADMYHVKRSLVGECIQRSKEVLYGSISAEVHALHRDGEVRSSGLVTEDTRV 474
            +LV++  LFP  ++G + ++       +P L  L V++++        SV + VA+   F +   V V +V+ +      +L  V L FK QYIGR DM+ +K++LV  C+  +++V +  I A+   L   GE  + G ++EDTRV
Sbjct:   22 ELVVNPKLFPQVSLGDIIEIAHPTDEYSPLL--LQVKSLKDDLQKETISVDQTVAQAFKFRAYQDVIVNIVDPK----DVSLDLVELTFKDQYIGRGDMWRLKKTLVTTCVYVTQKVEFAGIRAQAGELWVKGEKVTCGYISEDTRV 162          
BLAST of mRNA_M-pyrifera_M_contig84623.19723.1 vs. uniprot
Match: A0A6J2UVG4_CHACN (GATOR complex protein DEPDC5 n=1 Tax=Chanos chanos TaxID=29144 RepID=A0A6J2UVG4_CHACN)

HSP 1 Score: 63.9 bits (154), Expect = 7.010e-9
Identity = 45/147 (30.61%), Postives = 77/147 (52.38%), Query Frame = 1
Query:   34 DLVLSASLFPASAVGKVAQLRQKRAPNAPALGFLTVQAVRQLPSNAAASVAKGVAEVLGFASRMAVEVELVEDQVSREAAALSSVVLLFKGQYIGRADMYHVKRSLVGECIQRSKEVLYGSISAEVHALHRDGEVRSSGLVTEDTRV 474
            +L+++  +FP  ++G + ++       +P L  L V+++R+       SV + VA+     +   V + +VE     +   L  V L FK QYIGR DM+ +K+SLV  C   +++V +  I A+   L   GE  + G ++EDTRV
Sbjct:   26 ELMMNPKVFPQVSLGDIVEIAHPNDEYSPLL--LQVKSLREDLQKETVSVDQTVAQAFKLRAYQDVFINIVE----AKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYVTQKVEFAGIRAQASELWVKGEKVTCGYISEDTRV 166          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig84623.19723.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A8C9TCL4_SCLFO4.900e-930.61DEP domain containing 5, GATOR1 subcomplex subunit... [more]
UPI0008781A925.030e-930.61GATOR complex protein DEPDC5 isoform X6 n=2 Tax=Sc... [more]
A0A8C9T722_SCLFO5.120e-930.61DEP domain containing 5, GATOR1 subcomplex subunit... [more]
A0A8C9S1F0_SCLFO5.130e-930.61DEP domain containing 5, GATOR1 subcomplex subunit... [more]
A0A8C9WQB8_SCLFO5.140e-930.61DEP domain containing 5, GATOR1 subcomplex subunit... [more]
A0A8C9RXC2_SCLFO5.150e-930.61DEP domain containing 5, GATOR1 subcomplex subunit... [more]
A0A8C9S679_SCLFO5.160e-930.61DEP domain containing 5, GATOR1 subcomplex subunit... [more]
UPI000A1C72137.000e-931.29DEP domain-containing protein 5 n=1 Tax=Boleophtha... [more]
UPI0004954B717.000e-931.29GATOR complex protein DEPDC5 isoform X1 n=3 Tax=Cy... [more]
A0A6J2UVG4_CHACN7.010e-930.61GATOR complex protein DEPDC5 n=1 Tax=Chanos chanos... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig84623contigM-pyrifera_M_contig84623:34..558 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeMetazoa
Seed ortholog score77.0
Seed ortholog evalue8.6e-12
Seed eggNOG ortholog7739.XP_002591874.1
Preferred nameDEPDC5
KEGG koko:K20404
KEGG Pathwayko04150,map04150
Hectar predicted targeting categoryother localisation
GOsGO:0000323,GO:0003674,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005764,GO:0005765,GO:0005773,GO:0005774,GO:0005829,GO:0006950,GO:0007154,GO:0008150,GO:0009267,GO:0009605,GO:0009894,GO:0009966,GO:0009968,GO:0009987,GO:0009991,GO:0010506,GO:0010646,GO:0010648,GO:0016020,GO:0019222,GO:0023051,GO:0023057,GO:0031090,GO:0031323,GO:0031329,GO:0031667,GO:0031668,GO:0031669,GO:0032006,GO:0032007,GO:0032991,GO:0033554,GO:0034198,GO:0035859,GO:0042594,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044422,GO:0044424,GO:0044437,GO:0044444,GO:0044446,GO:0044464,GO:0044877,GO:0048471,GO:0048519,GO:0048523,GO:0048583,GO:0048585,GO:0050789,GO:0050794,GO:0050896,GO:0051716,GO:0065007,GO:0071496,GO:0098588,GO:0098805,GO:0098852,GO:1902531,GO:1902532,GO:1903432,GO:1904262,GO:1990130,GO:1990928
EggNOG free text desc.negative regulation of TORC1 signaling
EggNOG OGs38E71@33154,3BDWM@33208,3CRB3@33213,484NC@7711,KOG3572@1,KOG3572@2759
COG Functional cat.T
Best tax levelChordata
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001
Exons2
Model size474
Cds size474
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816753.3217843-CDS-M-pyrifera_M_contig84623:33..3931622816753.3217843-CDS-M-pyrifera_M_contig84623:33..393Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig84623 34..393 -
1692278157.9955637-CDS-M-pyrifera_M_contig84623:33..3931692278157.9955637-CDS-M-pyrifera_M_contig84623:33..393Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig84623 34..393 -
1622816753.3334384-CDS-M-pyrifera_M_contig84623:444..5581622816753.3334384-CDS-M-pyrifera_M_contig84623:444..558Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig84623 445..558 -
1692278158.008911-CDS-M-pyrifera_M_contig84623:444..5581692278158.008911-CDS-M-pyrifera_M_contig84623:444..558Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig84623 445..558 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig84623.19723.1prot_M-pyrifera_M_contig84623.19723.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig84623 34..558 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig84623.19723.1

>prot_M-pyrifera_M_contig84623.19723.1 ID=prot_M-pyrifera_M_contig84623.19723.1|Name=mRNA_M-pyrifera_M_contig84623.19723.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=158bp
LVLHDPFHEPLDLVLSASLFPASAVGKVAQLRQKRAPNAPALGFLTVQAV
RQLPSNAAASVAKGVAEVLGFASRMAVEVELVEDQVSREAAALSSVVLLF
KGQYIGRADMYHVKRSLVGECIQRSKEVLYGSISAEVHALHRDGEVRSSG
LVTEDTRV
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mRNA from alignment at M-pyrifera_M_contig84623:34..558-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig84623.19723.1 ID=mRNA_M-pyrifera_M_contig84623.19723.1|Name=mRNA_M-pyrifera_M_contig84623.19723.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=525bp|location=Sequence derived from alignment at M-pyrifera_M_contig84623:34..558- (Macrocystis pyrifera P11B4 male)
CTGGTGCTGCACGACCCCTTCCACGAGCCGCTGGACTTGGTGCTGAGCGC GAGCCTCTTCCCGGCCTCTGCGGTGGGGAAGGTCGCGCAGCTGCGGCAGA AGCGGGCGCCTAACGTCAGCCTCCACGCTCTGCTGGACGAGCTGGGCCAG GAGCAGAGCGAGGAGGCGCCTGCGTTGGGCTTCCTCACGGTGCAGGCGGT GCGGCAGCTGCCCTCCAATGCGGCTGCGAGCGTCGCCAAGGGCGTGGCAG AGGTGTTGGGCTTTGCCTCGCGCATGGCAGTGGAGGTGGAGCTGGTGGAG GACCAGGTGAGCAGGGAGGCCGCCGCACTCTCCTCCGTGGTGCTGCTCTT CAAGGGCCAGTACATTGGCAGGGCCGACATGTACCACGTCAAGCGCAGCC TCGTCGGCGAGTGCATCCAGCGCTCCAAGGAGGTGCTCTACGGCTCCATC TCTGCAGAGGTGCATGCCCTCCACCGCGACGGCGAAGTCCGCAGCTCCGG CCTCGTCACCGAGGACACCCGCGTA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig84623:34..558-

>mRNA_M-pyrifera_M_contig84623.19723.1 ID=mRNA_M-pyrifera_M_contig84623.19723.1|Name=mRNA_M-pyrifera_M_contig84623.19723.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=948bp|location=Sequence derived from alignment at M-pyrifera_M_contig84623:34..558- (Macrocystis pyrifera P11B4 male)
CTGGTGCTGCACGACCCCTTCCACGAGCCGCTGGACTTGGTGCTGAGCGC
GAGCCTCTTCCCGGCCTCTGCGGTGGGGAAGGTCGCGCAGCTGCGGCAGA
AGCGGGCGCCTAACCTGGTGCTGCACGACCCCTTCCACGAGCCGCTGGAC
TTGGTGCTGAGCGCGAGCCTCTTCCCGGCCTCTGCGGTGGGGAAGGTCGC
GCAGCTGCGGCAGAAGCGGGCGCCTAACGCGCCTGCGTTGGGCTTCCTCA
CGGTGCAGGCGGTGCGGCAGCTGCCCTCCAATGCGGCTGCGAGCGTCGCC
AAGGGCGTGGCAGAGGTGTTGGGCTTTGCCTCGCGCATGGCAGTGGAGGT
GGAGCTGGTGGAGGACCAGGTGAGCAGGGAGGCCGCCGCACTCTCCTCCG
TGGTGCTGCTCTTCAAGGGCCAGTACATTGGCAGGGCCGACATGTACCAC
GTCAAGCGCAGCCTCGTCGGCGAGTGCATCCAGCGCTCCAAGGAGGTGCT
CTACGGCTCCATCTCTGCAGAGGTGCATGCCCTCCACCGCGACGGCGAAG
TCCGCAGCTCCGGCCTCGTCACCGAGGACACCCGCGTAGCGCCTGCGTTG
GGCTTCCTCACGGTGCAGGCGGTGCGGCAGCTGCCCTCCAATGCGGCTGC
GAGCGTCGCCAAGGGCGTGGCAGAGGTGTTGGGCTTTGCCTCGCGCATGG
CAGTGGAGGTGGAGCTGGTGGAGGACCAGGTGAGCAGGGAGGCCGCCGCA
CTCTCCTCCGTGGTGCTGCTCTTCAAGGGCCAGTACATTGGCAGGGCCGA
CATGTACCACGTCAAGCGCAGCCTCGTCGGCGAGTGCATCCAGCGCTCCA
AGGAGGTGCTCTACGGCTCCATCTCTGCAGAGGTGCATGCCCTCCACCGC
GACGGCGAAGTCCGCAGCTCCGGCCTCGTCACCGAGGACACCCGCGTA
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