mRNA_M-pyrifera_M_contig83357.19466.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig83357.19466.1
Unique NamemRNA_M-pyrifera_M_contig83357.19466.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig83357.19466.1 vs. uniprot
Match: A0A8J5XDG9_DIALT (FtsJ domain-containing protein n=1 Tax=Diacronema lutheri TaxID=2081491 RepID=A0A8J5XDG9_DIALT)

HSP 1 Score: 75.1 bits (183), Expect = 4.480e-13
Identity = 55/153 (35.95%), Postives = 71/153 (46.41%), Query Frame = 1
Query:    1 IDLHPKRFRHVLTFIYESDAGKMTCSVSPSELFYNFDLRSARDASGTGVCRASFKLSETV-----------------HRLSHGRSEGEGSSVPALDLTKIDRALDIGAAPGGWTAVLVALGVGEVIAIDPGMLDDDVLAMPGVKHMIMRVETA 408
            + L PKR RH          G +   V P+E +   DL S    + T   RA +KL E +                        +EG G +V A  L +  RA+D+GAAPGGWT+ L+  G   V A+DP  LD  VL +PGV HM MR E A
Sbjct:  271 VRLEPKRGRHTHVVALAWANGPLAWGVEPAEAYGFKDLDSFLADAATS--RAYYKLREALVMHFGAAAXXXXXXXXXXXXXXXPAEGAGRAV-APPLLRGLRAVDVGAAPGGWTSCLIEHGAAHVTAVDPAELDAQVLVLPGVAHMRMRAEEA 420          
BLAST of mRNA_M-pyrifera_M_contig83357.19466.1 vs. uniprot
Match: A0A316DCD2_9BACL (23S rRNA (Cytidine2498-2'-O)-methyltransferase n=1 Tax=Tumebacillus permanentifrigoris TaxID=378543 RepID=A0A316DCD2_9BACL)

HSP 1 Score: 62.4 bits (150), Expect = 1.020e-8
Identity = 34/81 (41.98%), Postives = 48/81 (59.26%), Query Frame = 1
Query:  145 VCRASFKLSETVHRLSHGRSEGEGSSVPALDLTKIDRALDIGAAPGGWTAVLVALGVGEVIAIDPGMLDDDVLAMPGVKHM 387
            + RA FKL E   R               LDL   +RALDIGAAPGGW+++L+  G+ +V A+DPG +  D+L+ P +KH+
Sbjct:  182 ISRAVFKLLEAEERF-------------GLDLASFERALDIGAAPGGWSSLLLKRGL-QVTAVDPGEMHADLLSNPNLKHL 248          
BLAST of mRNA_M-pyrifera_M_contig83357.19466.1 vs. uniprot
Match: A0A7S0J109_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Calcidiscus leptoporus TaxID=127549 RepID=A0A7S0J109_9EUKA)

HSP 1 Score: 62.4 bits (150), Expect = 1.030e-8
Identity = 39/90 (43.33%), Postives = 49/90 (54.44%), Query Frame = 1
Query:  145 VCRASFKLSETVHRLSHGRSEGEGSSVPALDLTKIDRALDIGAAPGGWTAVLVALGVGEVIAIDPGMLDDDVLAMPGVKHMIMRVETATP 414
            VCRA+ K+ E   R    R  G  S+           A+D+GAAPGGWT+ L   G  EVIAIDPG L    +  P VKHM M+++ A P
Sbjct:  195 VCRAAHKIREVAARELL-RPLGASST-----------AIDVGAAPGGWTSYLADAGCAEVIAIDPGELRLGQVPRPAVKHMRMKLQDALP 272          
BLAST of mRNA_M-pyrifera_M_contig83357.19466.1 vs. uniprot
Match: A0A835XVX0_9CHLO (FtsJ domain-containing protein n=1 Tax=Edaphochlamys debaryana TaxID=47281 RepID=A0A835XVX0_9CHLO)

HSP 1 Score: 60.5 bits (145), Expect = 5.830e-8
Identity = 40/119 (33.61%), Postives = 61/119 (51.26%), Query Frame = 1
Query:   55 DAGKMTCSVSPSELFYNFDLRSARDASGTG-VCRASFKLSETVHRLSHGRSEGEGSSVPALDLTKIDRALDIGAAPGGWTAVLVALGVGEVIAIDPGMLDDDVLAMPGVKHMIMRVETA 408
            +  + +  ++P++  Y +   S RD    G +CRA+ KL E +   +     G     P L       A+D+GAAPGGWT +L A G   V++IDP  +D  V A+P V H+  R + A
Sbjct:  413 EVAQYSYGLAPADWQYQYG--SDRDKKMPGQLCRAASKLREALICCT-----GSAELRPGL------AAVDVGAAPGGWTQMLAAAGARLVVSIDPAQMDPGVAALPNVTHLQCRCDEA 518          
BLAST of mRNA_M-pyrifera_M_contig83357.19466.1 vs. uniprot
Match: U5QKS4_9CYAN (RNA 2'-O-ribose methyltransferase n=1 Tax=Gloeobacter kilaueensis JS1 TaxID=1183438 RepID=U5QKS4_9CYAN)

HSP 1 Score: 59.7 bits (143), Expect = 9.820e-8
Identity = 37/88 (42.05%), Postives = 48/88 (54.55%), Query Frame = 1
Query:  145 VCRASFKLSETVHRLSHGRSEGEGSSVPALDLTKIDRALDIGAAPGGWTAVLVALGVGEVIAIDPGMLDDDVLAMPGVKHMIMRVETA 408
            + RA+FKL E   R               LDL +  RALDIGAAPGGWTA+L+  G+ EV AIDP  L   +L  P ++++     TA
Sbjct:  186 ISRAAFKLLEAEERF-------------RLDLGRFRRALDIGAAPGGWTALLLDRGL-EVTAIDPAALHPTLLGHPRLRYLATSAATA 259          
BLAST of mRNA_M-pyrifera_M_contig83357.19466.1 vs. uniprot
Match: A0A7U6KH23_9FIRM (LSU rRNA 2'-O-methyl-C2498 methyltransferase RlmM n=1 Tax=Lachnospiraceae bacterium KM106-2 TaxID=2093742 RepID=A0A7U6KH23_9FIRM)

HSP 1 Score: 58.2 bits (139), Expect = 3.170e-7
Identity = 40/125 (32.00%), Postives = 60/125 (48.00%), Query Frame = 1
Query:   28 HVLTFIYESDAGKMTCSVSPSELFYNFDLRSARDASGTGVCRASFKLSETVHRLSHGRSEGEGSSVPALDLTKIDRALDIGAAPGGWTAVLVALGVGEVIAIDPGMLDDDVLAMPGVKHMIMRVE 402
            HV++ + +     +  S++   L +  +      +    +CRA  KL E + +    R EGEG+            ALD+GAAPGGWT VL   G  +V A+DPG LD+ +     + H   RVE
Sbjct:  144 HVISVVVKDKICYLGYSLAEDNLNFQCNEYRVLASEPIQICRAENKLKEAICKF-QVRLEGEGN------------ALDVGAAPGGWTKVLADYGY-QVSAVDPGKLDERLNTYSNITHYRDRVE 254          
BLAST of mRNA_M-pyrifera_M_contig83357.19466.1 vs. uniprot
Match: L8GJZ6_ACACA (Ribosomal RNA large subunit methyltransferase J, putative n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8GJZ6_ACACA)

HSP 1 Score: 58.2 bits (139), Expect = 3.460e-7
Identity = 47/134 (35.07%), Postives = 66/134 (49.25%), Query Frame = 1
Query:    1 IDLHPKRFRHVLTFIYESDAGKMTCSVSPSELFYNFDLRSARDASGTGVCRASFKLSETVHRLSHGRSEGEGSSVPALDLTKIDRALDIGAAPGGWTAVLVALGVGEVIAIDPGMLDDDVLAMPGVKHMIMRVE 402
            + LHPK+F HVL+   E   G+    ++P ELF+  +           V RA  K+ E   R+   R          L  T    A+D+GAAPGGWT  L  L + +VI+IDPG L  ++   P V H+  +VE
Sbjct:  207 VQLHPKQFTHVLSIARER--GRYYYGLAPKELFWGPES-GVHSVQMDKVSRAYHKMKEVFTRIDCLR----------LADTAQWTAIDVGAAPGGWTQYLSPL-MKQVISIDPGELRIEL--PPNVVHLKKKVE 324          
BLAST of mRNA_M-pyrifera_M_contig83357.19466.1 vs. uniprot
Match: A0A074LNG5_9BACL (SAM-dependent methyltransferase n=1 Tax=Tumebacillus flagellatus TaxID=1157490 RepID=A0A074LNG5_9BACL)

HSP 1 Score: 57.4 bits (137), Expect = 5.900e-7
Identity = 32/81 (39.51%), Postives = 45/81 (55.56%), Query Frame = 1
Query:  145 VCRASFKLSETVHRLSHGRSEGEGSSVPALDLTKIDRALDIGAAPGGWTAVLVALGVGEVIAIDPGMLDDDVLAMPGVKHM 387
            V RA FKL E   R               LD    +RALDIGAAPGGW+++L+  G+ +V A+DPG +D ++   P + H+
Sbjct:  182 VSRAKFKLLEAEERF-------------GLDFASFERALDIGAAPGGWSSLLLERGL-QVTAVDPGHMDAELAKHPKLTHL 248          
BLAST of mRNA_M-pyrifera_M_contig83357.19466.1 vs. uniprot
Match: A0A6U3T7A3_9STRA (Hypothetical protein n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A6U3T7A3_9STRA)

HSP 1 Score: 56.6 bits (135), Expect = 7.450e-7
Identity = 34/83 (40.96%), Postives = 46/83 (55.42%), Query Frame = 1
Query:  166 LSETVHRLSHGRSEGEGSSVPALDLTKIDR-ALDIGAAPGGWTAVLVA-LGVGEVIAIDPGMLDDDVLAMPGVKHMIMRVETA 408
            +SE+V        +  G S     + K  R ALD GAAPGGWT VL+  L    VIA+DP  LDD+V ++ GV+H    ++ A
Sbjct:   64 VSESVSATDKNSDDASGGSGLLCHMLKGSRVALDCGAAPGGWTQVLLKELDASVVIAVDPAELDDEVASLAGVEHWKCGIDEA 146          
BLAST of mRNA_M-pyrifera_M_contig83357.19466.1 vs. uniprot
Match: A0A3B0CD55_9BACL (Methyltransferase domain-containing protein n=1 Tax=Paenibacillus ginsengarvi TaxID=400777 RepID=A0A3B0CD55_9BACL)

HSP 1 Score: 57.0 bits (136), Expect = 8.140e-7
Identity = 35/81 (43.21%), Postives = 45/81 (55.56%), Query Frame = 1
Query:  145 VCRASFKLSETVHRLSHGRSEGEGSSVPALDLTKIDRALDIGAAPGGWTAVLVALGVGEVIAIDPGMLDDDVLAMPGVKHM 387
            V RA FKL E  +                LDL++  +ALDIGAAPGGWT++L+  GV EV A+DP  L  D+L  P +  M
Sbjct:  188 VSRAKFKLLEAEYEF-------------GLDLSRYAKALDIGAAPGGWTSLLLERGV-EVTAVDPADLHPDLLGHPKLTFM 254          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig83357.19466.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A8J5XDG9_DIALT4.480e-1335.95FtsJ domain-containing protein n=1 Tax=Diacronema ... [more]
A0A316DCD2_9BACL1.020e-841.9823S rRNA (Cytidine2498-2'-O)-methyltransferase n=1... [more]
A0A7S0J109_9EUKA1.030e-843.33Hypothetical protein (Fragment) n=1 Tax=Calcidiscu... [more]
A0A835XVX0_9CHLO5.830e-833.61FtsJ domain-containing protein n=1 Tax=Edaphochlam... [more]
U5QKS4_9CYAN9.820e-842.05RNA 2'-O-ribose methyltransferase n=1 Tax=Gloeobac... [more]
A0A7U6KH23_9FIRM3.170e-732.00LSU rRNA 2'-O-methyl-C2498 methyltransferase RlmM ... [more]
L8GJZ6_ACACA3.460e-735.07Ribosomal RNA large subunit methyltransferase J, p... [more]
A0A074LNG5_9BACL5.900e-739.51SAM-dependent methyltransferase n=1 Tax=Tumebacill... [more]
A0A6U3T7A3_9STRA7.450e-740.96Hypothetical protein n=1 Tax=Dictyocha speculum Ta... [more]
A0A3B0CD55_9BACL8.140e-743.21Methyltransferase domain-containing protein n=1 Ta... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig83357contigM-pyrifera_M_contig83357:415..831 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeViridiplantae
Seed ortholog score63.2
Seed ortholog evalue1.1e-07
Seed eggNOG ortholog3075.A0A087SR67
Hectar predicted targeting categoryother localisation
EggNOG free text desc.FtsJ-like methyltransferase
EggNOG OGs2E2U5@1,2SA0J@2759,34NMZ@3041,38AFC@33090
Ec32 ortholog descriptionS-adenosyl-L-methionine-dependent methyltransferase
Ec32 orthologEc-05_002080.1
COG Functional cat.S
Best tax levelChlorophyta
Best eggNOG OGNA|NA|NA
Exons1
Model size417
Cds size417
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816740.499038-CDS-M-pyrifera_M_contig83357:414..8311622816740.499038-CDS-M-pyrifera_M_contig83357:414..831Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig83357 415..831 +
1692278150.389882-CDS-M-pyrifera_M_contig83357:414..8311692278150.389882-CDS-M-pyrifera_M_contig83357:414..831Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig83357 415..831 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig83357.19466.1prot_M-pyrifera_M_contig83357.19466.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig83357 415..831 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig83357.19466.1

>prot_M-pyrifera_M_contig83357.19466.1 ID=prot_M-pyrifera_M_contig83357.19466.1|Name=mRNA_M-pyrifera_M_contig83357.19466.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=139bp
IDLHPKRFRHVLTFIYESDAGKMTCSVSPSELFYNFDLRSARDASGTGVC
RASFKLSETVHRLSHGRSEGEGSSVPALDLTKIDRALDIGAAPGGWTAVL
VALGVGEVIAIDPGMLDDDVLAMPGVKHMIMRVETATPV
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mRNA from alignment at M-pyrifera_M_contig83357:415..831+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig83357.19466.1 ID=mRNA_M-pyrifera_M_contig83357.19466.1|Name=mRNA_M-pyrifera_M_contig83357.19466.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=417bp|location=Sequence derived from alignment at M-pyrifera_M_contig83357:415..831+ (Macrocystis pyrifera P11B4 male)
ATTGACCTTCACCCGAAACGGTTCCGGCATGTGCTGACGTTCATCTACGA GAGCGATGCCGGGAAGATGACATGCTCTGTGTCTCCCAGTGAGCTCTTCT ACAATTTTGACCTGCGCTCTGCTCGCGACGCGTCAGGGACAGGTGTCTGC AGGGCCTCTTTCAAGCTCTCTGAGACAGTGCATCGCCTCTCTCATGGAAG ATCAGAAGGAGAGGGATCATCTGTTCCTGCACTCGATCTCACGAAGATAG ATCGCGCCTTAGACATTGGTGCAGCCCCGGGAGGATGGACTGCAGTCTTG GTGGCGCTCGGGGTTGGCGAAGTGATTGCGATCGATCCAGGCATGCTTGA TGACGACGTCCTTGCCATGCCAGGTGTCAAGCACATGATAATGAGGGTTG AGACAGCTACACCGGTA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig83357:415..831+

>mRNA_M-pyrifera_M_contig83357.19466.1 ID=mRNA_M-pyrifera_M_contig83357.19466.1|Name=mRNA_M-pyrifera_M_contig83357.19466.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=834bp|location=Sequence derived from alignment at M-pyrifera_M_contig83357:415..831+ (Macrocystis pyrifera P11B4 male)
ATTGACCTTCACCCGAAACGGTTCCGGCATGTGCTGACGTTCATCTACGA
GAGCGATGCCGGGAAGATGACATGCTCTGTGTCTCCCAGTGAGCTCTTCT
ACAATTTTGACCTGCGCTCTGCTCGCGACGCGTCAGGGACAGGTGTCTGC
AGGGCCTCTTTCAAGCTCTCTGAGACAGTGCATCGCCTCTCTCATGGAAG
ATCAGAAGGAGAGGGATCATCTGTTCCTGCACTCGATCTCACGAAGATAG
ATCGCGCCTTAGACATTGGTGCAGCCCCGGGAGGATGGACTGCAGTCTTG
GTGGCGCTCGGGGTTGGCGAAGTGATTGCGATCGATCCAGGCATGCTTGA
TGACGACGTCCTTGCCATGCCAGGTGTCAAGCACATGATAATGAGGGTTG
AGACAGCTACACCGGTAATTGACCTTCACCCGAAACGGTTCCGGCATGTG
CTGACGTTCATCTACGAGAGCGATGCCGGGAAGATGACATGCTCTGTGTC
TCCCAGTGAGCTCTTCTACAATTTTGACCTGCGCTCTGCTCGCGACGCGT
CAGGGACAGGTGTCTGCAGGGCCTCTTTCAAGCTCTCTGAGACAGTGCAT
CGCCTCTCTCATGGAAGATCAGAAGGAGAGGGATCATCTGTTCCTGCACT
CGATCTCACGAAGATAGATCGCGCCTTAGACATTGGTGCAGCCCCGGGAG
GATGGACTGCAGTCTTGGTGGCGCTCGGGGTTGGCGAAGTGATTGCGATC
GATCCAGGCATGCTTGATGACGACGTCCTTGCCATGCCAGGTGTCAAGCA
CATGATAATGAGGGTTGAGACAGCTACACCGGTA
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