mRNA_M-pyrifera_M_contig81261.19004.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig81261.19004.1
Unique NamemRNA_M-pyrifera_M_contig81261.19004.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig81261.19004.1 vs. uniprot
Match: A0A5A8CRW7_CAFRO (Leucine--tRNA ligase n=3 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8CRW7_CAFRO)

HSP 1 Score: 86.3 bits (212), Expect = 3.590e-17
Identity = 46/124 (37.10%), Postives = 65/124 (52.42%), Query Frame = 1
Query:    1 YRDICNRIGLPLHPVLMKRFANVACVTIAPICPHWAEEVYEVIGRPGGYPTVTAAPWPSTSSEEAALADAGRWLEQVLHACRDAAKSARRVAEKKKGKGTAPSGPIDTIRIYAVSDFTTWQKTV 372
            YRD C ++G  +H  L   F +   + IAPICPHWAEEV+E IG PGG  ++  A WP   + +AA+   G WL  + H+ R +  +  + A K   KG A +   +   IY    +  WQ  V
Sbjct:  932 YRDACEKLGEEMHGELAAWFMHAQAIAIAPICPHWAEEVWETIGCPGGAKSIHEARWPDGVAADAAVLSRGTWLLGLAHSIRASVDAEVKKAAK---KGDADAS-FNACTIYTRKAYQDWQVAV 1051          
BLAST of mRNA_M-pyrifera_M_contig81261.19004.1 vs. uniprot
Match: A0A024GMT2_9STRA (Leucine--tRNA ligase n=1 Tax=Albugo candida TaxID=65357 RepID=A0A024GMT2_9STRA)

HSP 1 Score: 68.6 bits (166), Expect = 5.690e-11
Identity = 40/127 (31.50%), Postives = 65/127 (51.18%), Query Frame = 1
Query:    1 YRDICNRIGLPLHPVLMKRFANVACVTIAPICPHWAEEVYEVIGRPGGYPTVTAAPWPSTSSEEAALADAGRWLEQVLHACRDA-AKSARRVAEKKKGKGTAPSGPIDTIR----IYAVSDFTTWQK 366
            YRD+C R  + +H  ++ R+ N   + ++PICPH+ E ++ ++G  G    V+   WP   S + +L  AG +L + +   RD  AKS+     K   KG   S  + T R    IY  ++F  WQ+
Sbjct:  847 YRDLCTRGEIAMHSKVLDRYVNAQTIMLSPICPHFCEHIWSIMGNSG---FVSTTSWPIVDSVDHSLLRAGDFLGKTIRHFRDIQAKSSGNNRSKSSTKG---SEAVPTKRSHAHIYLATEFPLWQQ 967          
BLAST of mRNA_M-pyrifera_M_contig81261.19004.1 vs. uniprot
Match: E4X998_OIKDI (Leucine--tRNA ligase n=1 Tax=Oikopleura dioica TaxID=34765 RepID=E4X998_OIKDI)

HSP 1 Score: 60.8 bits (146), Expect = 2.820e-8
Identity = 37/122 (30.33%), Postives = 60/122 (49.18%), Query Frame = 1
Query:    1 YRDICNRIGLPLHPVLMKRFANVACVTIAPICPHWAEEVYEVIGRPGGYPTVTAAPWPSTSSEEAALADAGRWLEQVLHACRDAAKSARRVAEKKKGKGTAPSGPIDTIRIYAVSDFTTWQK 366
            YR+IC   G+  +  L+ RF  V  + I PICPH AEEV+E+ G+ G      + P+P     +  L ++  +L + +   RD     +   + KKGK  AP+       +Y   ++  WQ+
Sbjct:  787 YREICGSEGM--NAKLVDRFIEVQTLLICPICPHIAEEVWEITGKKG---FAVSTPFPEILEYDPVLIESSEFLAETV---RDVRLKLKDRLQPKKGK--APAEIPTNCTVYVAKEYPAWQR 898          
BLAST of mRNA_M-pyrifera_M_contig81261.19004.1 vs. uniprot
Match: A0A8J9UK77_9NEOP ((lesser marbled fritillary) hypothetical protein n=1 Tax=Brenthis ino TaxID=405034 RepID=A0A8J9UK77_9NEOP)

HSP 1 Score: 60.8 bits (146), Expect = 2.830e-8
Identity = 33/124 (26.61%), Postives = 56/124 (45.16%), Query Frame = 1
Query:    1 YRDICNRIGLPLHPVLMKRFANVACVTIAPICPHWAEEVYEVIGRPGGYPTVTAAPWPSTSSEEAALADAGRWLEQVLHACRDAAKSARRVAEKKKGKGTAPSGPIDTIRIYAVSDFTTWQKTV 372
            YR++C   G+  H  L  R+  V    +AP+CPH AE V++++G      ++    WP     +     A  +L +  H+ R   K+   V + KKG+   P    +   I+   +F  WQ  +
Sbjct:  830 YRELCGEAGV--HAALAARYGAVQAALLAPVCPHVAEHVWQLLGNKE---SILHERWPVAGEVDEVAVKASNYLMEAAHSFRIYLKNHCAVKKPKKGETVKPEPKPNKAIIWVAKEFPKWQHII 948          
BLAST of mRNA_M-pyrifera_M_contig81261.19004.1 vs. uniprot
Match: A0A7S4BH41_CHRCT (Leucine--tRNA ligase n=1 Tax=Chrysotila carterae TaxID=13221 RepID=A0A7S4BH41_CHRCT)

HSP 1 Score: 60.1 bits (144), Expect = 5.280e-8
Identity = 36/110 (32.73%), Postives = 58/110 (52.73%), Query Frame = 1
Query:   46 LMKRFANVACVTIAPICPHWAEEVYEVIGRPGGYPTVTAAPWPSTSSEEAALADAGRWLEQVLHACRDAAKSARRVAEKKKGKGTAPSGPI-DTIRIYAVSDFTTWQKTV 372
            L++++     + +API PHW EE++E++G  G    V  A WP+   E+  ++ AG++L  + HA   A      V + KK KG  P GP  + + +Y  S F  W+  V
Sbjct:  959 LVQQWGEWQALMMAPITPHWCEEIWEIVGGKG---CVVRARWPTPRQEDPKISAAGQYLFGLTHALSTAV-----VNKDKKSKG--PQGPKPNQVNLYLASSFPRWKVLV 1058          
BLAST of mRNA_M-pyrifera_M_contig81261.19004.1 vs. uniprot
Match: A0A7S0QC49_9EUKA (Leucine--tRNA ligase (Fragment) n=1 Tax=Coccolithus braarudii TaxID=221442 RepID=A0A7S0QC49_9EUKA)

HSP 1 Score: 59.7 bits (143), Expect = 7.160e-8
Identity = 39/125 (31.20%), Postives = 62/125 (49.60%), Query Frame = 1
Query:    1 YRDICNRIGLPLHPVLMKRFANVACVTIAPICPHWAEEVYEVIGRPGGYPTVTAAPWPSTSS-EEAALADAGRWLEQVLHACRDAAKSARRVAEKKKGKGTAPSGPIDTIRIYAVSDFTTWQKTV 372
            YR    ++GL     L++++A    + +API PHW EE++  +G+ G    V  A WPS S  E+  +  AG +L  + H    A  +     +KKK KG A     + + +Y  S F  W++ V
Sbjct:  977 YRTGTAQVGL--LESLLRQWAEWQAIVMAPITPHWCEELWCKLGKQG---CVVKARWPSPSQPEQPEITAAGHYLFGLAHTLSTALVN----RDKKKAKGPAEEHKPNQVNLYVASSFPRWKELV 1092          
BLAST of mRNA_M-pyrifera_M_contig81261.19004.1 vs. uniprot
Match: UPI00103FEDA6 (leucine--tRNA ligase, cytoplasmic n=1 Tax=Ostrinia furnacalis TaxID=93504 RepID=UPI00103FEDA6)

HSP 1 Score: 59.7 bits (143), Expect = 7.170e-8
Identity = 34/124 (27.42%), Postives = 57/124 (45.97%), Query Frame = 1
Query:    1 YRDICNRIGLPLHPVLMKRFANVACVTIAPICPHWAEEVYEVIGRPGGYPTVTAAPWPSTSSEEAALADAGRWLEQVLHACRDAAKSARRVAEKKKGKGTAPSGPIDTIRIYAVSDFTTWQKTV 372
            YR++C  +GL  H  L+ R+       +APICPH AE V+E++G      ++    WP +   +     A  +L    H+ R   K+   V + KKG+   P    +   I+   ++  WQ  +
Sbjct:  832 YRELCADVGL--HANLLTRYIETQAKLMAPICPHVAEHVWELLGNKR---SILHERWPVSGDIDEVAVKASNYLMDAAHSFRIYLKNHCAVKKPKKGEAPKPEKKPNKAIIWVAKEYPRWQHII 950          
BLAST of mRNA_M-pyrifera_M_contig81261.19004.1 vs. uniprot
Match: D7FTL8_ECTSI (Leucine--tRNA ligase n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FTL8_ECTSI)

HSP 1 Score: 59.3 bits (142), Expect = 9.740e-8
Identity = 36/122 (29.51%), Postives = 61/122 (50.00%), Query Frame = 1
Query:    1 YRDICNRIGLPLHPVLMKRFANVACVTIAPICPHWAEEVYEVIGRPGGYPTVTAAPWPSTSSEEAALADAGRWLEQVLHACRDAAKSARRVAEKKKGKGTAPSGPIDTIRIYAVSDFTTWQK 366
            YRD C +    +H  L+ RF  V  + +AP+CPH+AE  + ++G  G   +V  A WP T   +  ++ + ++L + L       K+ R  A+K KG   AP     +  +Y  S +  W++
Sbjct:  886 YRDWCLKTSTLMHKTLILRFMEVQILLLAPMCPHYAEHFWGLLGH-GESGSVLKASWPQTGEVDGWMSRSFQFLSKTL-------KAFRLTAQKSKG---APK----SAHVYVASAYPQWKQ 992          
BLAST of mRNA_M-pyrifera_M_contig81261.19004.1 vs. uniprot
Match: A0A0D2WYV3_CAPO3 (Leucine--tRNA ligase n=2 Tax=Capsaspora owczarzaki (strain ATCC 30864) TaxID=595528 RepID=A0A0D2WYV3_CAPO3)

HSP 1 Score: 58.9 bits (141), Expect = 1.300e-7
Identity = 36/123 (29.27%), Postives = 58/123 (47.15%), Query Frame = 1
Query:    4 RDICNRIGLPLHPVLMKRFANVACVTIAPICPHWAEEVYEVIGRPGGYPTVTAAPWPSTSSEEAALADAGRWLEQVLHACRDAAKSARRVAEKKKGKGTAPSGPIDTIRIYAVSDFTTWQKTV 372
            RD    +    +  +++RF  V+ + IAPICPH+AE V+ ++GR         APWP   + +       R++    H+ R  A++   VA+  KGKG A         I+    +  WQ+ V
Sbjct:  616 RDFYREVSSAPNRRVLERFLEVSSILIAPICPHYAEHVFSLLGRKT---LAINAPWPVAGAVDELTLKMARYISTTAHSFR--ARAQLVVAKGNKGKGPAVELKNLNGTIFYAKSYPAWQEHV 733          
BLAST of mRNA_M-pyrifera_M_contig81261.19004.1 vs. uniprot
Match: A0A7S1D882_CYCTE (Leucine--tRNA ligase n=1 Tax=Cyclophora tenuis TaxID=216820 RepID=A0A7S1D882_CYCTE)

HSP 1 Score: 56.6 bits (135), Expect = 8.130e-7
Identity = 25/78 (32.05%), Postives = 43/78 (55.13%), Query Frame = 1
Query:    1 YRDICNRIGLPLHPVLMKRFANVACVTIAPICPHWAEEVYEVIGRPGGYPTVTAAPWPSTSSEEAALADAGRWLEQVL 234
            YR  C    +P+H  +++++A    V I PICPHW+E++++V+G+ G       A WP++  E+  L     +L   L
Sbjct:  411 YRSWCQDGAVPMHHDVIRKWAESLVVMICPICPHWSEKLWKVLGKEG---FAVKASWPTSDHEDKVLTRQAAFLRSSL 485          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig81261.19004.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A5A8CRW7_CAFRO3.590e-1737.10Leucine--tRNA ligase n=3 Tax=Cafeteria roenbergens... [more]
A0A024GMT2_9STRA5.690e-1131.50Leucine--tRNA ligase n=1 Tax=Albugo candida TaxID=... [more]
E4X998_OIKDI2.820e-830.33Leucine--tRNA ligase n=1 Tax=Oikopleura dioica Tax... [more]
A0A8J9UK77_9NEOP2.830e-826.61(lesser marbled fritillary) hypothetical protein n... [more]
A0A7S4BH41_CHRCT5.280e-832.73Leucine--tRNA ligase n=1 Tax=Chrysotila carterae T... [more]
A0A7S0QC49_9EUKA7.160e-831.20Leucine--tRNA ligase (Fragment) n=1 Tax=Coccolithu... [more]
UPI00103FEDA67.170e-827.42leucine--tRNA ligase, cytoplasmic n=1 Tax=Ostrinia... [more]
D7FTL8_ECTSI9.740e-829.51Leucine--tRNA ligase n=2 Tax=Ectocarpus TaxID=2879... [more]
A0A0D2WYV3_CAPO31.300e-729.27Leucine--tRNA ligase n=2 Tax=Capsaspora owczarzaki... [more]
A0A7S1D882_CYCTE8.130e-732.05Leucine--tRNA ligase n=1 Tax=Cyclophora tenuis Tax... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig81261contigM-pyrifera_M_contig81261:1..372 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score82.8
Seed ortholog evalue1.2e-13
Seed eggNOG ortholog67593.Physo133404
Preferred nameLARS
KEGG rclassRC00055,RC00523
KEGG koko:K01869
KEGG ReactionR03657
KEGG Pathwayko00970,map00970
KEGG ModuleM00359,M00360
Hectar predicted targeting categoryother localisation
GOsGO:0000323,GO:0001101,GO:0003674,GO:0003824,GO:0004812,GO:0004819,GO:0004823,GO:0004832,GO:0005096,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005737,GO:0005764,GO:0005773,GO:0005783,GO:0005829,GO:0005911,GO:0006082,GO:0006139,GO:0006399,GO:0006412,GO:0006418,GO:0006425,GO:0006429,GO:0006438,GO:0006518,GO:0006520,GO:0006605,GO:0006622,GO:0006623,GO:0006725,GO:0006807,GO:0006810,GO:0006886,GO:0006950,GO:0007034,GO:0007041,GO:0007154,GO:0008047,GO:0008104,GO:0008150,GO:0008152,GO:0008361,GO:0009058,GO:0009059,GO:0009267,GO:0009506,GO:0009507,GO:0009536,GO:0009605,GO:0009719,GO:0009892,GO:0009894,GO:0009895,GO:0009966,GO:0009967,GO:0009987,GO:0009991,GO:0010033,GO:0010243,GO:0010467,GO:0010506,GO:0010507,GO:0010646,GO:0010647,GO:0012505,GO:0015031,GO:0015833,GO:0016043,GO:0016070,GO:0016604,GO:0016874,GO:0016875,GO:0017101,GO:0019222,GO:0019538,GO:0019752,GO:0023051,GO:0023056,GO:0030054,GO:0030234,GO:0030695,GO:0031323,GO:0031324,GO:0031329,GO:0031330,GO:0031667,GO:0031668,GO:0031669,GO:0031974,GO:0031981,GO:0032006,GO:0032008,GO:0032535,GO:0032991,GO:0033036,GO:0033365,GO:0033554,GO:0034198,GO:0034613,GO:0034641,GO:0034645,GO:0034660,GO:0042221,GO:0042594,GO:0042886,GO:0043038,GO:0043039,GO:0043043,GO:0043085,GO:0043087,GO:0043170,GO:0043200,GO:0043201,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043436,GO:0043547,GO:0043603,GO:0043604,GO:0044093,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044271,GO:0044281,GO:0044422,GO:0044424,GO:0044428,GO:0044444,GO:0044446,GO:0044451,GO:0044464,GO:0045184,GO:0046483,GO:0046907,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048583,GO:0048584,GO:0050789,GO:0050790,GO:0050794,GO:0050896,GO:0051179,GO:0051234,GO:0051336,GO:0051345,GO:0051641,GO:0051649,GO:0051716,GO:0055044,GO:0060589,GO:0061462,GO:0065007,GO:0065008,GO:0065009,GO:0070013,GO:0070727,GO:0070887,GO:0071229,GO:0071230,GO:0071233,GO:0071310,GO:0071417,GO:0071495,GO:0071496,GO:0071702,GO:0071704,GO:0071705,GO:0071840,GO:0072594,GO:0072665,GO:0072666,GO:0090066,GO:0090304,GO:0098772,GO:0140098,GO:0140101,GO:1901360,GO:1901564,GO:1901566,GO:1901576,GO:1901698,GO:1901699,GO:1901700,GO:1901701,GO:1902531,GO:1902533,GO:1903432,GO:1904263,GO:1990253,GO:1990928
EggNOG free text desc.Anticodon-binding domain of tRNA
EggNOG OGs3Q8ZA@4776,COG0495@1,KOG0437@2759
EC6.1.1.4
COG Functional cat.J
Best tax levelPeronosporales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko01007,ko03016,ko03029
Exons1
Model size372
Cds size372
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816718.5819027-CDS-M-pyrifera_M_contig81261:0..3721622816718.5819027-CDS-M-pyrifera_M_contig81261:0..372Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig81261 1..372 -
1692278138.3702123-CDS-M-pyrifera_M_contig81261:0..3721692278138.3702123-CDS-M-pyrifera_M_contig81261:0..372Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig81261 1..372 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig81261.19004.1prot_M-pyrifera_M_contig81261.19004.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig81261 1..372 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig81261.19004.1

>prot_M-pyrifera_M_contig81261.19004.1 ID=prot_M-pyrifera_M_contig81261.19004.1|Name=mRNA_M-pyrifera_M_contig81261.19004.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=124bp
YRDICNRIGLPLHPVLMKRFANVACVTIAPICPHWAEEVYEVIGRPGGYP
TVTAAPWPSTSSEEAALADAGRWLEQVLHACRDAAKSARRVAEKKKGKGT
APSGPIDTIRIYAVSDFTTWQKTV
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mRNA from alignment at M-pyrifera_M_contig81261:1..372-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig81261.19004.1 ID=mRNA_M-pyrifera_M_contig81261.19004.1|Name=mRNA_M-pyrifera_M_contig81261.19004.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=372bp|location=Sequence derived from alignment at M-pyrifera_M_contig81261:1..372- (Macrocystis pyrifera P11B4 male)
TACCGTGATATCTGCAACCGTATTGGCCTCCCACTGCACCCAGTCCTGAT GAAGCGATTTGCAAATGTTGCATGTGTCACTATTGCACCAATCTGTCCAC ACTGGGCAGAAGAAGTGTATGAAGTGATTGGTCGCCCAGGTGGATATCCC ACCGTCACTGCAGCTCCATGGCCATCCACCTCCTCGGAAGAAGCTGCACT TGCTGATGCAGGACGCTGGCTGGAACAAGTGCTTCATGCTTGTCGTGATG CCGCAAAGAGTGCTCGTCGAGTTGCGGAGAAGAAGAAAGGAAAAGGAACA GCTCCGTCGGGACCAATTGACACAATTCGGATCTATGCAGTCTCTGACTT CACCACATGGCAAAAGACTGTT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig81261:1..372-

>mRNA_M-pyrifera_M_contig81261.19004.1 ID=mRNA_M-pyrifera_M_contig81261.19004.1|Name=mRNA_M-pyrifera_M_contig81261.19004.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=744bp|location=Sequence derived from alignment at M-pyrifera_M_contig81261:1..372- (Macrocystis pyrifera P11B4 male)
TACCGTGATATCTGCAACCGTATTGGCCTCCCACTGCACCCAGTCCTGAT
GAAGCGATTTGCAAATGTTGCATGTGTCACTATTGCACCAATCTGTCCAC
ACTGGGCAGAAGAAGTGTATGAAGTGATTGGTCGCCCAGGTGGATATCCC
ACCGTCACTGCAGCTCCATGGCCATCCACCTCCTCGGAAGAAGCTGCACT
TGCTGATGCAGGACGCTGGCTGGAACAAGTGCTTCATGCTTGTCGTGATG
CCGCAAAGAGTGCTCGTCGAGTTGCGGAGAAGAAGAAAGGAAAAGGAACA
GCTCCGTCGGGACCAATTGACACAATTCGGATCTATGCAGTCTCTGACTT
CACCACATGGCAAAAGACTGTTTACCGTGATATCTGCAACCGTATTGGCC
TCCCACTGCACCCAGTCCTGATGAAGCGATTTGCAAATGTTGCATGTGTC
ACTATTGCACCAATCTGTCCACACTGGGCAGAAGAAGTGTATGAAGTGAT
TGGTCGCCCAGGTGGATATCCCACCGTCACTGCAGCTCCATGGCCATCCA
CCTCCTCGGAAGAAGCTGCACTTGCTGATGCAGGACGCTGGCTGGAACAA
GTGCTTCATGCTTGTCGTGATGCCGCAAAGAGTGCTCGTCGAGTTGCGGA
GAAGAAGAAAGGAAAAGGAACAGCTCCGTCGGGACCAATTGACACAATTC
GGATCTATGCAGTCTCTGACTTCACCACATGGCAAAAGACTGTT
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