mRNA_M-pyrifera_M_contig81142.18980.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig81142.18980.1
Unique NamemRNA_M-pyrifera_M_contig81142.18980.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig81142.18980.1 vs. uniprot
Match: A0A7S2UM25_9STRA (Hypothetical protein n=1 Tax=Attheya septentrionalis TaxID=420275 RepID=A0A7S2UM25_9STRA)

HSP 1 Score: 116 bits (290), Expect = 2.220e-27
Identity = 68/183 (37.16%), Postives = 106/183 (57.92%), Query Frame = 1
Query:   31 VGKGVQRVIVVEKECVMHRLVEDRWPETSGSVLVSGKGMPDLPSRALIHALHTIHHLPVKGLCDCGPFGVAILAVFRYGSNTPG--DGHRFQTPLHWLGLQPSDASILP--LPPHTHQTLTKSDVSKTQSLLHDSRLHPSWKMQVKRYWDQQDAWKC--------ELEALLACGLHGLEDFLN 543
            V +  + ++V+EKE V +RL EDR+ E    +LV+GKG PDL +RAL+H+LH    LPV GLCDC P+GV +L  ++ GS   G   G R+  P+ W+GL+PS  + +   LP   +Q+LT+ D  + +SL  ++    +    V   ++++D  +C        ELEAL   G+  + D+L 
Sbjct:  227 VSQHAKCILVIEKEGVYNRLSEDRFFERYPCILVTGKGFPDLATRALVHSLHHELQLPVYGLCDCNPYGVGVLQTYQRGSTKMGLDGGSRYTVPIQWIGLRPSQVASMKADLPRDVYQSLTELDERRIESLCRETSPFTN----VLNGYERRDELRCMAKAGYKVELEALHWLGMDFMCDWLE 405          
BLAST of mRNA_M-pyrifera_M_contig81142.18980.1 vs. uniprot
Match: A0A6A3F4G6_9STRA (TP6A_N domain-containing protein n=2 Tax=Phytophthora fragariae TaxID=53985 RepID=A0A6A3F4G6_9STRA)

HSP 1 Score: 114 bits (285), Expect = 4.790e-27
Identity = 59/175 (33.71%), Postives = 100/175 (57.14%), Query Frame = 1
Query:   28 EVGKGVQRVIVVEKECVMHRLVEDRWPETSGSVLVSGKGMPDLPSRALIHALHTIHHLPVKGLCDCGPFGVAILAVFRYGS-NTPGDGHRFQTPLHWLGLQPSDASILPLPPHTHQTLTKSDVSKTQSLLHDS--RLHPSWKMQVKRYWDQQDAWKCELEALLACGLHGLEDFLN 543
            +V    + ++VVEK+ + +RL ED++ E   S+L++G+G PDL +R  +  L     +PV GLCDC PFG++I+  ++ GS   P +   +   + W+G++PS  + L LP  + + LT+ D++   SLL  S  + +  ++ +V+ + +    WK ELEAL   G   L  FL 
Sbjct:  183 DVQSNAKYIVVVEKDGIFNRLREDKFYEMVPSILITGRGFPDLATRVFVSLLSHALKIPVLGLCDCNPFGLSIMLTYKLGSARMPLESLEYAVDIKWIGVRPSQVATLGLPQSSFKALTQKDIAVANSLLQASFVQANEHYRREVRMWLEDSLPWKIELEALHFLGFTFLTSFLQ 357          
BLAST of mRNA_M-pyrifera_M_contig81142.18980.1 vs. uniprot
Match: A0A7S2JQ12_9STRA (Hypothetical protein n=1 Tax=Leptocylindrus danicus TaxID=163516 RepID=A0A7S2JQ12_9STRA)

HSP 1 Score: 112 bits (281), Expect = 1.740e-26
Identity = 67/174 (38.51%), Postives = 102/174 (58.62%), Query Frame = 1
Query:   52 VIVVEKECVMHRLVEDRWPETSGSVLVSGKGMPDLPSRALIHALHTIHHLPVKGLCDCGPFGVAILAVFRYGSNTPG-DGHRFQTPLHWLGLQPSDASILP--LPPHTHQTLTKSDVSKTQSLLHDS----RLHPSWKMQVKRYWDQQDAWKCELEALLACGLHGLEDFLNTKL 552
            ++ VEKE +  RL EDR+ +    +L++GKG PDL SRA + ALH + ++PV GLCDC PFGV++L  +  GS+  G D   +  P+ WLGL+PS    L   LP    Q LT +D  K  SL+ ++      +P  K ++  +  Q++ +K ELE+L   GL  L ++L  ++
Sbjct:  186 ILCVEKEGIYTRLSEDRFFDKVPCILITGKGFPDLASRACVFALHNMLNIPVYGLCDCNPFGVSVLMTYWRGSSKLGNDADSYSVPVKWLGLRPSHIEELSPELPQEVFQNLTNTDFRKLDSLMSETCDFVDKNPERKEEL--FEMQENGYKLELESLQWIGLDFLTNWLIDRI 357          
BLAST of mRNA_M-pyrifera_M_contig81142.18980.1 vs. uniprot
Match: W7TGS0_9STRA (Meiotic recombination protein spo11 isoform 2 n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7TGS0_9STRA)

HSP 1 Score: 113 bits (282), Expect = 4.980e-26
Identity = 67/169 (39.64%), Postives = 95/169 (56.21%), Query Frame = 1
Query:   52 VIVVEKECVMHRLVEDRW-PETSGSVLVSGKGMPDLPSRALIHALHTIHHLPVKGLCDCGPFGVAILAVFRYGSNTPGDGHRFQTPLHWLGLQPSDASI-LPLPPHTHQTLTKSDVSKTQSLLHDS--RLHPSWKMQVKRYWDQQDAWKCELEALLA-CGLHGLEDFLN 543
            ++VVEKEC+  RL+ D    +  G VL++GKG PDL +RA + AL     +PV GLCDC P G+A+L  ++ GS    +   ++ PL WLGL+PS       LP  T Q  ++ D+SK  SL+     + H  W+ +V+    +    KCELEAL    G  G  DFL+
Sbjct:  307 IVVVEKECMFKRLIGDGLLGQAGGIVLITGKGYPDLATRACVKALVDAFAVPVVGLCDCNPHGIALLLTYKCGSFRMPESQAYRIPLCWLGLRPSQLKRRFDLPDDTFQAFSEGDISKCSSLMDSPFCQAHLPWREEVETMASEH--LKCELEALNTFSGSKGRRDFLS 473          
BLAST of mRNA_M-pyrifera_M_contig81142.18980.1 vs. uniprot
Match: D0MT30_PHYIT (TP6A_N domain-containing protein n=2 Tax=Phytophthora infestans TaxID=4787 RepID=D0MT30_PHYIT)

HSP 1 Score: 109 bits (272), Expect = 2.820e-25
Identity = 61/173 (35.26%), Postives = 97/173 (56.07%), Query Frame = 1
Query:   34 GKGVQRVIVVEKECVMHRLVEDRWPETSGSVLVSGKGMPDLPSRALIHALHTIHHLPVKGLCDCGPFGVAILAVFRYGS-NTPGDGHRFQTPLHWLGLQPSDASILPLPPHTHQTLTKSDVSKTQSLLHDSRLHPS--WKMQVKRYWDQQDAWKCELEALLACGLHGLEDFLN 543
            G G +  I  E   +    +ED++ ET  S+L++G+G PDL +RA ++ L     +PV GLCDC PFG++I+  ++ GS   P D  R+   + W+G++PS  S L LP  + + LT+ D++   SL+  S +  +  +K +V+ +      WK ELEAL + G   L  FL 
Sbjct:  168 GSGEEVSITHELLQIKPHDIEDKFFETVPSILITGRGFPDLATRAFVNLLSNALGIPVLGLCDCNPFGLSIMLTYKLGSARMPLDSLRYAVDIKWVGVRPSQVSHLGLPTSSFKALTRKDLAVADSLMRASFIQANAFFKDEVQMWLGDALPWKIELEALYSSGFTFLTSFLQ 340          
BLAST of mRNA_M-pyrifera_M_contig81142.18980.1 vs. uniprot
Match: W2KQB5_PHYPR (TP6A_N domain-containing protein n=2 Tax=Phytophthora parasitica TaxID=4792 RepID=W2KQB5_PHYPR)

HSP 1 Score: 110 bits (274), Expect = 2.830e-25
Identity = 57/167 (34.13%), Postives = 97/167 (58.08%), Query Frame = 1
Query:   52 VIVVEKECVMHRLVEDRWPETSGSVLVSGKGMPDLPSRALIHALHTIHHLPVKGLCDCGPFGVAILAVFRYGS-NTPGDGHRFQTPLHWLGLQPSDASILPLPPHTHQTLTKSDVSKTQSLLHDSRLHPS--WKMQVKRYWDQQDAWKCELEALLACGLHGLEDFLN 543
            ++V+EK+ + +RL ED++ E   S+L++G+G PDL +R  +  L +   +PV GLCDC PFG++I+  ++ GS   P +  ++   + W+G++PS  + L LP  + + LT+ D++   SL   S +  +  +K +V+ +      WK ELEAL   G   L  FL 
Sbjct:  223 ILVIEKDGIFNRLREDKFFEIVPSILITGRGFPDLATRVFVSLLRSALEIPVLGLCDCNPFGLSIMLTYKLGSARMPLESLQYAVDIKWVGVRPSQIADLDLPISSFKALTRKDLAVADSLFRSSFIQANDHFKDEVQMWLGDALPWKIELEALHVLGFTFLTSFLQ 389          
BLAST of mRNA_M-pyrifera_M_contig81142.18980.1 vs. uniprot
Match: A0A0P1AB95_PLAHL (Type ii dna topoisomerase vi subunit a 2 n=1 Tax=Plasmopara halstedii TaxID=4781 RepID=A0A0P1AB95_PLAHL)

HSP 1 Score: 108 bits (270), Expect = 5.900e-25
Identity = 57/175 (32.57%), Postives = 98/175 (56.00%), Query Frame = 1
Query:   28 EVGKGVQRVIVVEKECVMHRLVEDRWPETSGSVLVSGKGMPDLPSRALIHALHTIHHLPVKGLCDCGPFGVAILAVFRYGS-NTPGDGHRFQTPLHWLGLQPSDASILPLPPHTHQTLTKSDVSKTQSLLHDSRLHPS--WKMQVKRYWDQQDAWKCELEALLACGLHGLEDFLN 543
            E+    + ++V+EK+ + +RL ED++ +   S+LV+GKG PDL +R  +  L     +PV G+CDC PFG++I+  ++ GS   P +  ++   + W+G++PS  S + LP  + + L++ DV+   SL+  S +     ++ +V  +      WK ELEAL   G   L  FL 
Sbjct:  171 ELRSNAKIILVIEKDGIFNRLREDKFFDVVPSILVTGKGFPDLATRVFVSLLSKALRIPVLGVCDCNPFGLSIILTYKLGSARMPLESLQYAVDIKWIGVRPSQISNIALPASSFKVLSRKDVAVANSLMRASFIQADMHFRNEVHMWLGDALPWKIELEALHCLGFTFLTSFLQ 345          
BLAST of mRNA_M-pyrifera_M_contig81142.18980.1 vs. uniprot
Match: B7G1L8_PHATC (Type II DNA topoisomerase VI subunit n=2 Tax=Phaeodactylum tricornutum TaxID=2850 RepID=B7G1L8_PHATC)

HSP 1 Score: 108 bits (271), Expect = 1.090e-24
Identity = 56/122 (45.90%), Postives = 79/122 (64.75%), Query Frame = 1
Query:   52 VIVVEKECVMHRLVEDRWPETSGSVLVSGKGMPDLPSRALIHALH-TIHHLPVKGLCDCGPFGVAILAVFRYGSNTPGDG-HRFQTPLHWLGLQPSDASILPLPPHTHQTLTKSDVSKTQSL 411
            ++V+EKE V +RLVEDR+ +    +LV+GKG PDL +RAL+H LH T+  LPV+GLCDC P+GV +L  +++ +    DG HRF  P+ W+GL+PS    L   P+T    +K      QSL
Sbjct:  228 ILVIEKEGVYNRLVEDRFFDRFPCILVTGKGFPDLSTRALVHVLHHTLGLLPVRGLCDCNPYGVMVLHTYQHTARKGVDGGHRFGVPISWIGLRPSQVQQLQRQPNTKHGQSKLPDQVFQSL 349          
BLAST of mRNA_M-pyrifera_M_contig81142.18980.1 vs. uniprot
Match: W2PU68_PHYPN (Apple domain-containing protein n=6 Tax=Phytophthora parasitica TaxID=4792 RepID=W2PU68_PHYPN)

HSP 1 Score: 109 bits (273), Expect = 1.100e-24
Identity = 57/167 (34.13%), Postives = 96/167 (57.49%), Query Frame = 1
Query:   52 VIVVEKECVMHRLVEDRWPETSGSVLVSGKGMPDLPSRALIHALHTIHHLPVKGLCDCGPFGVAILAVFRYGS-NTPGDGHRFQTPLHWLGLQPSDASILPLPPHTHQTLTKSDVSKTQSLLHDSRLHPS--WKMQVKRYWDQQDAWKCELEALLACGLHGLEDFLN 543
            ++V+EK+ + +RL ED++ E   S+L++G+G PDL +R  +  L     +PV GLCDC PFG++I+  ++ GS   P +  ++   + W+G++PS  + L LP  + + LT+ D++   SL   S +  +  +K +V+ +      WK ELEAL   G   L  FL 
Sbjct:  347 ILVIEKDGIFNRLREDKFFEIVPSILITGRGFPDLATRVFVSLLRNALEIPVLGLCDCNPFGLSIMLTYKLGSARMPLESLQYAVDIKWVGVRPSQIADLDLPISSFKALTRKDLAVADSLFRSSFIQANDHFKDEVQMWLGDALPWKIELEALHVLGFTFLTSFLQ 513          
BLAST of mRNA_M-pyrifera_M_contig81142.18980.1 vs. uniprot
Match: K0RG96_THAOC (TP6A_N domain-containing protein n=1 Tax=Thalassiosira oceanica TaxID=159749 RepID=K0RG96_THAOC)

HSP 1 Score: 110 bits (274), Expect = 1.190e-24
Identity = 60/131 (45.80%), Postives = 81/131 (61.83%), Query Frame = 1
Query:   34 GKGVQRVIVVEKECVMHRLVEDRWPETSGSVLVSGKGMPDLPSRALIHALHTIHHLPVKGLCDCGPFGVAILAVFRYGSNTPG-DGH-RFQTPLHWLGLQPSDASILP--LPPHTHQTLTKSDVSKTQSLL 414
             K  + ++V+EKE V +RL E+R  +    +LV+GKG PDL +RAL+HALH    LPV G+CDC PFGV++LA++ Y     G DG  R+  P+ W+GL+PSD   L   LP    Q LT  D  +  SLL
Sbjct:  489 SKDARLIVVIEKEGVYNRLSEERIFDRFPCILVTGKGFPDLATRALVHALHHELELPVVGICDCNPFGVSVLALYCYAGERMGIDGRMRYSVPIEWIGLRPSDVDGLKSELPGTVFQRLTDLDRKRITSLL 619          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig81142.18980.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S2UM25_9STRA2.220e-2737.16Hypothetical protein n=1 Tax=Attheya septentrional... [more]
A0A6A3F4G6_9STRA4.790e-2733.71TP6A_N domain-containing protein n=2 Tax=Phytophth... [more]
A0A7S2JQ12_9STRA1.740e-2638.51Hypothetical protein n=1 Tax=Leptocylindrus danicu... [more]
W7TGS0_9STRA4.980e-2639.64Meiotic recombination protein spo11 isoform 2 n=1 ... [more]
D0MT30_PHYIT2.820e-2535.26TP6A_N domain-containing protein n=2 Tax=Phytophth... [more]
W2KQB5_PHYPR2.830e-2534.13TP6A_N domain-containing protein n=2 Tax=Phytophth... [more]
A0A0P1AB95_PLAHL5.900e-2532.57Type ii dna topoisomerase vi subunit a 2 n=1 Tax=P... [more]
B7G1L8_PHATC1.090e-2445.90Type II DNA topoisomerase VI subunit n=2 Tax=Phaeo... [more]
W2PU68_PHYPN1.100e-2434.13Apple domain-containing protein n=6 Tax=Phytophtho... [more]
K0RG96_THAOC1.190e-2445.80TP6A_N domain-containing protein n=1 Tax=Thalassio... [more]

Pages

back to top
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig81142contigM-pyrifera_M_contig81142:297..848 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score113.2
Seed ortholog evalue1.3e-22
Seed eggNOG ortholog112098.XP_008607094.1
KEGG koko:K10878
KEGG Pathwayko04113,map04113
Hectar predicted targeting categoryother localisation
EggNOG free text desc.DNA topoisomerase VI subunit A
EggNOG OGsCOG1697@1,KOG2795@2759
Ec32 ortholog descriptionSporulation-specific protein 11 homologue
Ec32 orthologEc-03_004560.1
COG Functional cat.L
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko03400
Exons1
Model size552
Cds size552
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816717.1932578-CDS-M-pyrifera_M_contig81142:296..8481622816717.1932578-CDS-M-pyrifera_M_contig81142:296..848Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig81142 297..848 -
1692278137.4699986-CDS-M-pyrifera_M_contig81142:296..8481692278137.4699986-CDS-M-pyrifera_M_contig81142:296..848Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig81142 297..848 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig81142.18980.1prot_M-pyrifera_M_contig81142.18980.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig81142 297..848 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig81142.18980.1

>prot_M-pyrifera_M_contig81142.18980.1 ID=prot_M-pyrifera_M_contig81142.18980.1|Name=mRNA_M-pyrifera_M_contig81142.18980.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=184bp
ICAAEIPVIEVGKGVQRVIVVEKECVMHRLVEDRWPETSGSVLVSGKGMP
DLPSRALIHALHTIHHLPVKGLCDCGPFGVAILAVFRYGSNTPGDGHRFQ
TPLHWLGLQPSDASILPLPPHTHQTLTKSDVSKTQSLLHDSRLHPSWKMQ
VKRYWDQQDAWKCELEALLACGLHGLEDFLNTKL
back to top

mRNA from alignment at M-pyrifera_M_contig81142:297..848-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig81142.18980.1 ID=mRNA_M-pyrifera_M_contig81142.18980.1|Name=mRNA_M-pyrifera_M_contig81142.18980.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=552bp|location=Sequence derived from alignment at M-pyrifera_M_contig81142:297..848- (Macrocystis pyrifera P11B4 male)
ATTTGTGCAGCAGAAATCCCAGTGATAGAAGTGGGAAAAGGAGTGCAGCG AGTGATAGTCGTGGAGAAAGAATGTGTGATGCATCGTCTTGTGGAAGATC GTTGGCCAGAAACAAGTGGCAGCGTGTTGGTGTCTGGGAAAGGAATGCCT GATCTCCCCTCAAGAGCCCTCATTCACGCTCTTCACACGATCCATCACCT TCCAGTGAAAGGATTGTGTGATTGTGGCCCTTTCGGTGTCGCAATTCTCG CAGTGTTTCGATATGGATCAAATACTCCAGGGGATGGCCATCGATTCCAA ACTCCACTTCACTGGCTCGGTCTCCAACCATCTGATGCATCTATTCTCCC ACTCCCACCACACACCCACCAAACTCTCACAAAATCTGATGTATCCAAAA CCCAATCTCTACTCCATGATTCCCGTTTGCATCCTTCATGGAAAATGCAA GTGAAACGTTATTGGGATCAACAGGATGCGTGGAAGTGTGAACTTGAAGC TCTCCTTGCTTGTGGACTCCACGGTCTCGAAGACTTTCTCAACACCAAAC TT
back to top

Coding sequence (CDS) from alignment at M-pyrifera_M_contig81142:297..848-

>mRNA_M-pyrifera_M_contig81142.18980.1 ID=mRNA_M-pyrifera_M_contig81142.18980.1|Name=mRNA_M-pyrifera_M_contig81142.18980.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1104bp|location=Sequence derived from alignment at M-pyrifera_M_contig81142:297..848- (Macrocystis pyrifera P11B4 male)
ATTTGTGCAGCAGAAATCCCAGTGATAGAAGTGGGAAAAGGAGTGCAGCG
AGTGATAGTCGTGGAGAAAGAATGTGTGATGCATCGTCTTGTGGAAGATC
GTTGGCCAGAAACAAGTGGCAGCGTGTTGGTGTCTGGGAAAGGAATGCCT
GATCTCCCCTCAAGAGCCCTCATTCACGCTCTTCACACGATCCATCACCT
TCCAGTGAAAGGATTGTGTGATTGTGGCCCTTTCGGTGTCGCAATTCTCG
CAGTGTTTCGATATGGATCAAATACTCCAGGGGATGGCCATCGATTCCAA
ACTCCACTTCACTGGCTCGGTCTCCAACCATCTGATGCATCTATTCTCCC
ACTCCCACCACACACCCACCAAACTCTCACAAAATCTGATGTATCCAAAA
CCCAATCTCTACTCCATGATTCCCGTTTGCATCCTTCATGGAAAATGCAA
GTGAAACGTTATTGGGATCAACAGGATGCGTGGAAGTGTGAACTTGAAGC
TCTCCTTGCTTGTGGACTCCACGGTCTCGAAGACTTTCTCAACACCAAAC
TTATTTGTGCAGCAGAAATCCCAGTGATAGAAGTGGGAAAAGGAGTGCAG
CGAGTGATAGTCGTGGAGAAAGAATGTGTGATGCATCGTCTTGTGGAAGA
TCGTTGGCCAGAAACAAGTGGCAGCGTGTTGGTGTCTGGGAAAGGAATGC
CTGATCTCCCCTCAAGAGCCCTCATTCACGCTCTTCACACGATCCATCAC
CTTCCAGTGAAAGGATTGTGTGATTGTGGCCCTTTCGGTGTCGCAATTCT
CGCAGTGTTTCGATATGGATCAAATACTCCAGGGGATGGCCATCGATTCC
AAACTCCACTTCACTGGCTCGGTCTCCAACCATCTGATGCATCTATTCTC
CCACTCCCACCACACACCCACCAAACTCTCACAAAATCTGATGTATCCAA
AACCCAATCTCTACTCCATGATTCCCGTTTGCATCCTTCATGGAAAATGC
AAGTGAAACGTTATTGGGATCAACAGGATGCGTGGAAGTGTGAACTTGAA
GCTCTCCTTGCTTGTGGACTCCACGGTCTCGAAGACTTTCTCAACACCAA
ACTT
back to top