mRNA_M-pyrifera_M_contig776.18259.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig776.18259.1
Unique NamemRNA_M-pyrifera_M_contig776.18259.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig776.18259.1 vs. uniprot
Match: D7G710_ECTSI (General transcription and DNA repair factor IIH subunit TFB5 n=2 Tax=Ectocarpus TaxID=2879 RepID=D7G710_ECTSI)

HSP 1 Score: 132 bits (333), Expect = 1.040e-37
Identity = 64/69 (92.75%), Postives = 68/69 (98.55%), Query Frame = 1
Query:    1 MSTGFRSSKGVIITCDVPTKQFILSLDARLACVVSDLDATHVFVKKDSVKEIQRELDALHEKNVYVRPR 207
            MSTG+RSSKGV+ITCDVPTKQFI+SLDARLACVVSDLDATHVFVKKDSVKEIQREL+ LHEKNVYVRPR
Sbjct:    1 MSTGYRSSKGVLITCDVPTKQFIMSLDARLACVVSDLDATHVFVKKDSVKEIQRELEQLHEKNVYVRPR 69          
BLAST of mRNA_M-pyrifera_M_contig776.18259.1 vs. uniprot
Match: A0A835ZC95_9STRA (General transcription and DNA repair factor IIH subunit TFB5 n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZC95_9STRA)

HSP 1 Score: 74.7 bits (182), Expect = 7.250e-15
Identity = 38/69 (55.07%), Postives = 51/69 (73.91%), Query Frame = 1
Query:    1 MSTGFRSSKGVIITCDVPTKQFILSLDARLA--CVVSDLDATHVFVKKDSVKEIQRELDALHEKNVYVR 201
            MS    S KGV+ITCDVPTKQFIL L+++ A   +++DLD TH+FV+  +V EI+ +L+ L E NVYVR
Sbjct:    1 MSNVRTSRKGVLITCDVPTKQFILHLNSKQAQGFIINDLDETHLFVEASAVHEIREQLERLQEMNVYVR 69          
BLAST of mRNA_M-pyrifera_M_contig776.18259.1 vs. uniprot
Match: A0A2V3IN38_9FLOR (General transcription and DNA repair factor IIH subunit TFB5 n=1 Tax=Gracilariopsis chorda TaxID=448386 RepID=A0A2V3IN38_9FLOR)

HSP 1 Score: 59.7 bits (143), Expect = 1.340e-8
Identity = 27/61 (44.26%), Postives = 44/61 (72.13%), Query Frame = 1
Query:   19 SSKGVIITCDVPTKQFILSLDARLA--CVVSDLDATHVFVKKDSVKEIQRELDALHEKNVY 195
            ++ GV+I+CD+P KQF+L +D +     ++SDLD THV VK+ ++  I+ +LD L+E+N Y
Sbjct:   50 ATAGVLISCDIPVKQFLLHVDEKDTGKFIISDLDETHVLVKESALDYIKSQLDKLYEENQY 110          
BLAST of mRNA_M-pyrifera_M_contig776.18259.1 vs. uniprot
Match: A0A6T6HXU1_9STRA (General transcription and DNA repair factor IIH subunit TFB5 n=1 Tax=Craspedostauros australis TaxID=1486917 RepID=A0A6T6HXU1_9STRA)

HSP 1 Score: 57.0 bits (136), Expect = 1.330e-7
Identity = 28/63 (44.44%), Postives = 43/63 (68.25%), Query Frame = 1
Query:   19 SSKGVIITCDVPTKQFILSLDARLAC----VVSDLDATHVFVKKDSVKEIQRELDALHEKNVY 195
            +S G +ITCDVPTKQ+I  L+   A     ++ DLDATH+ VKK +  EI+R +++  ++NV+
Sbjct:   36 ASAGYLITCDVPTKQYIQYLNEMKAVDKKFIIEDLDATHLLVKKKARAEIERRVESWMDENVF 98          
BLAST of mRNA_M-pyrifera_M_contig776.18259.1 vs. uniprot
Match: M2VWH3_GALSU (General transcription and DNA repair factor IIH subunit TFB5 n=1 Tax=Galdieria sulphuraria TaxID=130081 RepID=M2VWH3_GALSU)

HSP 1 Score: 55.5 bits (132), Expect = 1.920e-7
Identity = 26/60 (43.33%), Postives = 43/60 (71.67%), Query Frame = 1
Query:   25 KGVIITCDVPTKQFILSLDARLA---CVVSDLDATHVFVKKDSVKEIQRELDALHEKNVY 195
            +GV+ITCD+P KQFIL LD   A    V+++LD T +F++  ++K +Q +L+ L+E+N +
Sbjct:    6 RGVLITCDIPMKQFILKLDEDQAPNRFVIANLDETSLFIQSWALKLVQEKLEELYEENYF 65          
BLAST of mRNA_M-pyrifera_M_contig776.18259.1 vs. uniprot
Match: A4S7H4_OSTLU (General transcription and DNA repair factor IIH subunit TFB5 n=1 Tax=Ostreococcus lucimarinus (strain CCE9901) TaxID=436017 RepID=A4S7H4_OSTLU)

HSP 1 Score: 53.5 bits (127), Expect = 1.210e-6
Identity = 30/63 (47.62%), Postives = 38/63 (60.32%), Query Frame = 1
Query:   28 GVIITCDVPTKQFILSLD----ARLACVVSDLDATHVFVKKDSVKEIQRELDALHEKNVYVRP 204
            GV+IT DVP KQ+IL L+    A    VV DLDA  V V+ D+V  I RE++A  +   YV P
Sbjct:    7 GVMITADVPIKQYILHLNDVAPAERKFVVVDLDARRVLVQPDAVDAIHREIEAFQQSTQYVAP 69          
BLAST of mRNA_M-pyrifera_M_contig776.18259.1 vs. uniprot
Match: I0Z2U2_COCSC (General transcription and DNA repair factor IIH subunit TFB5 n=2 Tax=Coccomyxa TaxID=41891 RepID=I0Z2U2_COCSC)

HSP 1 Score: 53.1 bits (126), Expect = 1.540e-6
Identity = 27/65 (41.54%), Postives = 41/65 (63.08%), Query Frame = 1
Query:   25 KGVIITCDVPTKQFILSLD----ARLACVVSDLDATHVFVKKDSVKEIQRELDALHEKNVYVRPR 207
            +GV++ CDVPTK+FILSL+         V+ DLD T +FV+   V+ +++ L   +E+N Y  PR
Sbjct:    2 RGVLLQCDVPTKEFILSLNDSKPTNERFVILDLDDTRLFVQPTVVEWLEKRLKEFNEENTYQPPR 66          
BLAST of mRNA_M-pyrifera_M_contig776.18259.1 vs. uniprot
Match: A0A1X6PEZ7_PORUM (General transcription and DNA repair factor IIH subunit TFB5 n=1 Tax=Porphyra umbilicalis TaxID=2786 RepID=A0A1X6PEZ7_PORUM)

HSP 1 Score: 51.2 bits (121), Expect = 7.970e-6
Identity = 26/62 (41.94%), Postives = 40/62 (64.52%), Query Frame = 1
Query:   19 SSKGVIITCDVPTKQFILSLDARLA---CVVSDLDATHVFVKKDSVKEIQRELDALHEKNVY 195
            ++ GV++TCDVP KQF+L LD   A    +V+ LD TH+F+   +V  I+ +L+ L  +N Y
Sbjct:    4 ATHGVLVTCDVPVKQFLLKLDNDRAPDRFIVAKLDETHLFIHAWAVDMIKDQLERLFAENQY 65          
BLAST of mRNA_M-pyrifera_M_contig776.18259.1 vs. uniprot
Match: A0A7R9C1N4_9CRUS (General transcription and DNA repair factor IIH subunit TFB5 n=1 Tax=Notodromas monacha TaxID=399045 RepID=A0A7R9C1N4_9CRUS)

HSP 1 Score: 50.1 bits (118), Expect = 2.410e-5
Identity = 22/54 (40.74%), Postives = 36/54 (66.67%), Query Frame = 1
Query:   22 SKGVIITCDVPTKQFILSLDARLAC----VVSDLDATHVFVKKDSVKEIQRELD 171
            SKGV++TCD P +QF++ LD +       ++ DLD+TH+F+  D V E+  ++D
Sbjct:    5 SKGVLVTCDPPMRQFLIWLDEKEKLGRKFILLDLDSTHLFISADVVNELMEKVD 58          
BLAST of mRNA_M-pyrifera_M_contig776.18259.1 vs. uniprot
Match: A0A1Y1ILP3_KLENI (General transcription and DNA repair factor IIH subunit TFB5 n=1 Tax=Klebsormidium nitens TaxID=105231 RepID=A0A1Y1ILP3_KLENI)

HSP 1 Score: 48.5 bits (114), Expect = 8.620e-5
Identity = 23/64 (35.94%), Postives = 39/64 (60.94%), Query Frame = 1
Query:   25 KGVIITCDVPTKQFILSLDARLA----CVVSDLDATHVFVKKDSVKEIQRELDALHEKNVYVRP 204
            KG++I CD+P KQFI+S++ +L      ++ DLD TH+FV+      +Q ++    +K  Y +P
Sbjct:    6 KGILIECDIPMKQFIVSINDQLPNAQKFIIYDLDDTHLFVQPHVADMLQNKVKDFLDKITYEKP 69          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig776.18259.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 10
Match NameE-valueIdentityDescription
D7G710_ECTSI1.040e-3792.75General transcription and DNA repair factor IIH su... [more]
A0A835ZC95_9STRA7.250e-1555.07General transcription and DNA repair factor IIH su... [more]
A0A2V3IN38_9FLOR1.340e-844.26General transcription and DNA repair factor IIH su... [more]
A0A6T6HXU1_9STRA1.330e-744.44General transcription and DNA repair factor IIH su... [more]
M2VWH3_GALSU1.920e-743.33General transcription and DNA repair factor IIH su... [more]
A4S7H4_OSTLU1.210e-647.62General transcription and DNA repair factor IIH su... [more]
I0Z2U2_COCSC1.540e-641.54General transcription and DNA repair factor IIH su... [more]
A0A1X6PEZ7_PORUM7.970e-641.94General transcription and DNA repair factor IIH su... [more]
A0A7R9C1N4_9CRUS2.410e-540.74General transcription and DNA repair factor IIH su... [more]
A0A1Y1ILP3_KLENI8.620e-535.94General transcription and DNA repair factor IIH su... [more]
back to top
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig776contigM-pyrifera_M_contig776:2821..5727 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionTFIIH subunit TTDA/Tfb5
Ec32 orthologEc-01_011510.1
Exons3
Model size491
Cds size210
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig776.18259.1prot_M-pyrifera_M_contig776.18259.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig776 2821..5446 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816676.7071853-CDS-M-pyrifera_M_contig776:2820..28641622816676.7071853-CDS-M-pyrifera_M_contig776:2820..2864Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig776 2821..2864 +
1692278113.5011544-CDS-M-pyrifera_M_contig776:2820..28641692278113.5011544-CDS-M-pyrifera_M_contig776:2820..2864Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig776 2821..2864 +
1622816676.7197285-CDS-M-pyrifera_M_contig776:4027..41411622816676.7197285-CDS-M-pyrifera_M_contig776:4027..4141Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig776 4028..4141 +
1692278113.5142777-CDS-M-pyrifera_M_contig776:4027..41411692278113.5142777-CDS-M-pyrifera_M_contig776:4027..4141Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig776 4028..4141 +
1622816676.730674-CDS-M-pyrifera_M_contig776:5394..54461622816676.730674-CDS-M-pyrifera_M_contig776:5394..5446Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig776 5395..5446 +
1692278113.524141-CDS-M-pyrifera_M_contig776:5394..54461692278113.524141-CDS-M-pyrifera_M_contig776:5394..5446Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig776 5395..5446 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816676.7412045-UTR-M-pyrifera_M_contig776:5446..57271622816676.7412045-UTR-M-pyrifera_M_contig776:5446..5727Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig776 5447..5727 +
1692278113.5343778-UTR-M-pyrifera_M_contig776:5446..57271692278113.5343778-UTR-M-pyrifera_M_contig776:5446..5727Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig776 5447..5727 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig776.18259.1

>prot_M-pyrifera_M_contig776.18259.1 ID=prot_M-pyrifera_M_contig776.18259.1|Name=mRNA_M-pyrifera_M_contig776.18259.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=70bp
MSTGFRSSKGVIITCDVPTKQFILSLDARLACVVSDLDATHVFVKKDSVK
EIQRELDALHEKNVYVRPR*
back to top

mRNA from alignment at M-pyrifera_M_contig776:2821..5727+

Legend: polypeptideCDSUTR
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig776.18259.1 ID=mRNA_M-pyrifera_M_contig776.18259.1|Name=mRNA_M-pyrifera_M_contig776.18259.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=2907bp|location=Sequence derived from alignment at M-pyrifera_M_contig776:2821..5727+ (Macrocystis pyrifera P11B4 male)
ATGAGCACTGGATTTCGCAGCAGCAAGGGGGTTATTATCACCTGGTATGA GGGCAAACAAACATGTGATACGATTTTTTTTTGTCAAGAATCCCCCCTCT GACCCATGAGCGCTTCGTAGCAGCACGGTGCCACAACAGGTAGACAACAG CAGTAGTGCTGCAGTAGAGCTACTCGACCAAGGTAGAGCGGCCTTATAGC TTCCTACTTGTGTGAATAGAACCAGCCATCACTAGTAACGCTCTGGGTTG ACGCTACTCTTATGTTTTTAGGTATATACTGTGATGACCTCTTCCCCAAG AGAGACGAGGTTGGTAACTTGAGTTTCTTGTTCTGTTACCATCAACAGGT GTTCTTGGGGGCTTGCACCAATGGCCGATCATTGCAATGGCCGAAGGTCC TCTTGCTCCAACCAATATTGTACTCGTTCAATCAAGTCAAGTTGCCGCTG CCGCTGTTTGGCTGTACGTATTGATAGCCTGTCGGTCCCAGACCAGGCAT GACACGTTGTTCGAGTTGCATCAATGTACACTTGGCACTTGATAAGCAGC CGAGACATATGGATGTAGGGTGACCGCAGCAAAAAAAAAATCATGAGGTG GCTCGCAGTGCGCTACTCCTATATTACTACGATGTATATTAATATTAATA TTATTACTAATATGTACCAGGTACTGAAGTATATATACCCTGGTGCGTAT GTTCGTGTTTTTTTCGCACTTTTTTTTCCAACTTTTCTCTTCGTACGAGC ACTTTACGGCCAAACCCCAAGTGCCCCGACACCGGCATGAATACAGGTAC AACAATCAATATTAATAGTAGTGGACAAGTTGGCCGATCTCGCTCGTCGT GTGGCGGGCCAAACGCACCTGGCCGTCACTCTCTCCGCCATAAAGCCCCG TGTCCGATATCTGCTATCCCGCTACCGCTACTGCAAGCTACTACTGCTGT AGCTATACTAGTGCGTTGATAGCACCCTCCCTTGCCATGCGTTGGTATGT ACCCCCTCTGTGATATTTCTGGGGTGTGTCTGCGGTGGTGTGGGTGTAGA TCCTACTGAAACTGAAACTGTACGACCATATACGACCTGGTACGAACTAT ATTATGAACTCGTTGAGCACTACTCTGTGGCAACCGTGCATGGTTCTCAT CTCTCGTTTCTCGCCCTCTATACCTTGAATTATATGTCTTCAATGCTTGC CGTCCAGCGATGTTCCCACGAAGCAGTTCATTCTGAGCCTGGATGCGCGC CTAGCATGCGTGGTGTCGGATCTGGACGCCACTCACGTCTTCGTCAAGAA AGACAGCGTCAAGGAAATACAGTGAGTAAGCTCAGGCTGAACCCTGCCAA GCCACTCTAAATCACCTCGTGACTTATCTCGCGCATCAGATGCCAAGTAC ATAAAAATTGCTCGACGAGTATATCTACGGTCCGTCTCGAATAATACAGC GTTGTGGCCGCATGGTTAATGGTCTCGAGGGATACATCCTCAGCGAAAGC ATCTATATTAGGATACCTGGTACCTGGTATGTCACTACAGCCTACTTGGA CCTGCAGCAGTAATGTCGAATGCTTCAAAGTTCCAACCGGGTGTTCGACA ACTTATTGTACTTTGTACTGTGATACCAGGTATATACAGTTGATTCGGCG CAGTTCTAGCGTATACCTGGTAGGTAGTAACGCCTTAACCCAGATCTTGT CACAATAAATCAAGAGATAGGTCGTACCAGGTATGAACTGTCTACGGGAA ATGTAGGCGCGCATGTACGAGCAGTAATATGTTGCGCCCCTGTCGTTTGC CCACTTGGAGTTTAGTTCGTCCTTGTAGTTTGTATGGTCCTCTAACCAAG CATTGGCCGGTGTTGTCATCGTCACTTTTTCTCTTTCAAGTCCCGGAAAC CTTCACTCACTCTCCTTGTGACATACGACTTTATTCACTGTCTCCTGTTT TGCGGCTCTATTAGATGGTTTCGTCACTCAAGACTCGCGACGCCTGTCAT GTGCACTGGTTCCACTGGTTCCAGCACTGTAGCTCCGCAGCATGTACCCA ACGCTTGACACACGTAGTACCTATAATACGAGAGCTACTACCATTTATCC CAGCCAAAGCGGTGTTAACAGTGACTTCATCCCATACGACGTTGAGTCTG TGTAGTTTAGTTTAGTTTCGACACCGAAGACCCAAAGCGGTGAGCGATTC CTCCAACATGGCAAGTGCCGCTTTGGATACATGCCAGGCTTTGTTGCACC AAGCTCTTGCGCGAATCCCAACATGGTCGGCCTTGGATCCTCGACGGTTG AGACAATGTGAAACCTTCCCCACATGCCATGTACACCCTTACCGATCCAA ATGCTTTCAACACTTCATCACTGCCCATAGTCGCTATGAATAGAGTTGTA CGTCATGCTCCCGTCCCTATGCGACACGTACCTACCAGGTATCATAAATA CCAGCATTCCGGTAGTACGAGTAGTACACTGCACGGTTGTGCCCGTATGC CCTGACCCTTCCGCCGCCGCGTTCCCCATATTTCCCACTCTACCAATGTG GATTGCTCGTTTGCGCTTTTCAAGGCGGGAACTGGACGCGCTACACGAGA AGAACGTCTACGTGCGACCTCGATAGCTCCGGCCGACCGGCGTGTTGTAT TTTTGTCATGATGATGATACATACGATCTTCAGCAGTCTTGACGAAGCTT GACCTGCGAACTGCGAAGCTTACCGCCATCCACCCGTCCGCCCGTTCGTG GGTGGACCGAGACCCAATGCGATCGTGTGTGTGTTTCCCGTGGTCACCAC ATCATACGTACGGCTGTGCTGCTAGAGTGTGAAGCAGCGTGCGTTGTAGA AAACCGCCTCTTGTCATGCGTTGCCGGTTGTAGTACTCTGCTTGATTTAG GTTATCG
back to top

Coding sequence (CDS) from alignment at M-pyrifera_M_contig776:2821..5727+

>mRNA_M-pyrifera_M_contig776.18259.1 ID=mRNA_M-pyrifera_M_contig776.18259.1|Name=mRNA_M-pyrifera_M_contig776.18259.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=420bp|location=Sequence derived from alignment at M-pyrifera_M_contig776:2821..5727+ (Macrocystis pyrifera P11B4 male)
ATGAGCACTGGATTTCGCAGCAGCAAGGGGGTTATTATCACCTGATGAGC
ACTGGATTTCGCAGCAGCAAGGGGGTTATTATCACCTGCGATGTTCCCAC
GAAGCAGTTCATTCTGAGCCTGGATGCGCGCCTAGCATGCGTGGTGTCGG
ATCTGGACGCCACTCACGTCTTCGTCAAGAAAGACAGCGTCAAGGAAATA
CACGATGTTCCCACGAAGCAGTTCATTCTGAGCCTGGATGCGCGCCTAGC
ATGCGTGGTGTCGGATCTGGACGCCACTCACGTCTTCGTCAAGAAAGACA
GCGTCAAGGAAATACAGCGGGAACTGGACGCGCTACACGAGAAGAACGTC
TACGTGCGACCTCGATAGGCGGGAACTGGACGCGCTACACGAGAAGAACG
TCTACGTGCGACCTCGATAG
back to top