mRNA_M-pyrifera_M_contig727.17269.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig727.17269.1
Unique NamemRNA_M-pyrifera_M_contig727.17269.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig727.17269.1 vs. uniprot
Match: D8LPP6_ECTSI (AP complex subunit sigma n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LPP6_ECTSI)

HSP 1 Score: 301 bits (770), Expect = 3.580e-103
Identity = 150/157 (95.54%), Postives = 152/157 (96.82%), Query Frame = 1
Query:    1 MIKFVLMVNKMGQTRVSSYYDWMPIPERVALEAEVVRRCFSRSELQCSFFEFRGYKIIYRRYASLFFIVGVGVDKDDENELGVLEFIHCLVETLDKYFQSVCELDIMFNLEKTHFILDEMVLNGYICEGNRENVLKPLLLMDKVAH-GDAGFGSKSS 468
            MIKFVLMVNKMGQTRVSSYYDWMPIPERVALEAEVVRRCFSRSELQCSFFEFRGYKIIYRRYASLFFIVGVGVD DDENELGVLEFIHCLVETLDKYFQSVCELDIMFNLEKTHFILDEMVLNGYICEGNRENVLKPLLLMD  +H GD+ FGSKSS
Sbjct:    1 MIKFVLMVNKMGQTRVSSYYDWMPIPERVALEAEVVRRCFSRSELQCSFFEFRGYKIIYRRYASLFFIVGVGVDSDDENELGVLEFIHCLVETLDKYFQSVCELDIMFNLEKTHFILDEMVLNGYICEGNRENVLKPLLLMDSSSHAGDSVFGSKSS 157          
BLAST of mRNA_M-pyrifera_M_contig727.17269.1 vs. uniprot
Match: A0A835Z9B6_9STRA (AP complex subunit sigma n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z9B6_9STRA)

HSP 1 Score: 276 bits (707), Expect = 1.400e-93
Identity = 138/156 (88.46%), Postives = 147/156 (94.23%), Query Frame = 1
Query:    1 MIKFVLMVNKMGQTRVSSYYDWMPIPERVALEAEVVRRCFSRSELQCSFFEFRGYKIIYRRYASLFFIVGVGVDKDDENELGVLEFIHCLVETLDKYFQSVCELDIMFNLEKTHFILDEMVLNGYICEGNRENVLKPLLLMDKV-AHGDAGFGSKS 465
            MIKFVLMVNKMGQTRVSSYY+W+PIPERVALEAEVVRRCFSRSELQCSFFEFRGYKIIYRRYASLFFIVGV    D+ENELG+LEFIHCLVETLDKYFQSVCELDIMFNLEKTHFILDEMV+NG +CE NR+N+LKPL LMDKV A+ DAGFGSKS
Sbjct:    1 MIKFVLMVNKMGQTRVSSYYEWLPIPERVALEAEVVRRCFSRSELQCSFFEFRGYKIIYRRYASLFFIVGVD-SSDEENELGMLEFIHCLVETLDKYFQSVCELDIMFNLEKTHFILDEMVMNGCVCEANRDNILKPLQLMDKVSANDDAGFGSKS 155          
BLAST of mRNA_M-pyrifera_M_contig727.17269.1 vs. uniprot
Match: A0A7S2UUI5_9STRA (AP complex subunit sigma n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2UUI5_9STRA)

HSP 1 Score: 236 bits (603), Expect = 8.640e-78
Identity = 112/154 (72.73%), Postives = 134/154 (87.01%), Query Frame = 1
Query:    1 MIKFVLMVNKMGQTRVSSYYDWMPIPERVALEAEVVRRCFSRSELQCSFFEFRGYKIIYRRYASLFFIVGVGVDKDDENELGVLEFIHCLVETLDKYFQSVCELDIMFNLEKTHFILDEMVLNGYICEGNRENVLKPLLLMDKVAHGDAGFGSK 462
            MIKF+LMVNK GQTR+SSY++WM IPERVALE+E++R+C SR+E QCSF E+RGY++IYRRYASLFFIVGVG D  +ENELGVLEFIH LVETLDKYF+SVCELDIMFN+EK HFILDEMV+NGYI E NR N+LKP+  MDK+A+ +    S+
Sbjct:    1 MIKFILMVNKQGQTRLSSYFEWMTIPERVALESEIIRKCLSRAEYQCSFLEYRGYRVIYRRYASLFFIVGVGAD--EENELGVLEFIHSLVETLDKYFESVCELDIMFNMEKAHFILDEMVMNGYIMETNRTNILKPIAQMDKMANSEDSIFSR 152          
BLAST of mRNA_M-pyrifera_M_contig727.17269.1 vs. uniprot
Match: F0Y567_AURAN (AP complex subunit sigma n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0Y567_AURAN)

HSP 1 Score: 227 bits (578), Expect = 5.560e-74
Identity = 105/144 (72.92%), Postives = 128/144 (88.89%), Query Frame = 1
Query:    4 IKFVLMVNKMGQTRVSSYYDWMPIPERVALEAEVVRRCFSRSELQCSFFEFRGYKIIYRRYASLFFIVGVGVDKDDENELGVLEFIHCLVETLDKYFQSVCELDIMFNLEKTHFILDEMVLNGYICEGNRENVLKPLLLMDKVA 435
            I+F+LMVNK GQTR+S+YY+W+P+PERVALE+E++R+C SRSE QCSF E+RGYK++YRRYASLFFIVG   ++D ENELG+LEFIH LVETLDKYF+SVCELDIMFNLEK HFILDEMV+NG I E N+ NVLKP+ LM+K +
Sbjct:    3 IRFILMVNKQGQTRLSTYYEWLPMPERVALESEIIRKCLSRSEFQCSFVEYRGYKVVYRRYASLFFIVGC--EQDSENELGILEFIHALVETLDKYFESVCELDIMFNLEKAHFILDEMVMNGCIVETNKANVLKPIALMEKAS 144          
BLAST of mRNA_M-pyrifera_M_contig727.17269.1 vs. uniprot
Match: A0A7S3ZZG7_9STRA (AP complex subunit sigma n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A7S3ZZG7_9STRA)

HSP 1 Score: 225 bits (573), Expect = 3.100e-73
Identity = 106/144 (73.61%), Postives = 127/144 (88.19%), Query Frame = 1
Query:    4 IKFVLMVNKMGQTRVSSYYDWMPIPERVALEAEVVRRCFSRSELQCSFFEFRGYKIIYRRYASLFFIVGVGVDKDDENELGVLEFIHCLVETLDKYFQSVCELDIMFNLEKTHFILDEMVLNGYICEGNRENVLKPLLLMDKVA 435
            IKFVLMVNK GQTR+S+YY+W+ +PERVALEAE++R C SRSE QCSF E+RGYK++YRRYASLFFIVGV  D+D ENELG+LEFIH LVETLDKYF+SVCELDIMFNLEK HFILDEMV++G + E N+ N+LKP+ LM+K +
Sbjct:    3 IKFVLMVNKQGQTRLSTYYEWLSMPERVALEAEIIRTCLSRSEFQCSFVEYRGYKVVYRRYASLFFIVGV--DQDGENELGILEFIHALVETLDKYFESVCELDIMFNLEKAHFILDEMVVDGCVVETNKVNILKPIALMEKAS 144          
BLAST of mRNA_M-pyrifera_M_contig727.17269.1 vs. uniprot
Match: A0A7S2FA09_9STRA (AP complex subunit sigma n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2FA09_9STRA)

HSP 1 Score: 224 bits (572), Expect = 8.850e-73
Identity = 111/154 (72.08%), Postives = 132/154 (85.71%), Query Frame = 1
Query:    1 MIKFVLMVNKMGQTRVSSYYDWMPIPERVALEAEVVRRCFSRSELQCSFFEFRGYKIIYRRYASLFFIVGVGVDKDDENELGVLEFIHCLVETLDKYFQSVCELDIMFNLEKTHFILDEMVLNGYICEGNRENVLKPLLLMDKVAHGDAGFGSK 462
            M+KFVLMVNK GQTR+SSYY+W+P+ ER ALEAE++R+C SR+E QCSF E+RGYK+IYRRYASLFFIVG+   KD+ NELGVLEFIH LVETLDKYF+SVCELDIMFNLEK HFIL+EMV NG I E N+ N+LKP++LM+K A  D+ F SK
Sbjct:   20 MLKFVLMVNKQGQTRLSSYYEWIPMQERCALEAEIIRKCLSRNETQCSFVEYRGYKVIYRRYASLFFIVGM---KDNMNELGVLEFIHALVETLDKYFESVCELDIMFNLEKAHFILEEMVQNGCIFETNKANILKPIMLMEK-ASDDSVFSSK 169          
BLAST of mRNA_M-pyrifera_M_contig727.17269.1 vs. uniprot
Match: A0A7S3GXI4_9STRA (AP complex subunit sigma n=1 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3GXI4_9STRA)

HSP 1 Score: 223 bits (567), Expect = 2.630e-72
Identity = 104/154 (67.53%), Postives = 130/154 (84.42%), Query Frame = 1
Query:    1 MIKFVLMVNKMGQTRVSSYYDWMPIPERVALEAEVVRRCFSRSELQCSFFEFRGYKIIYRRYASLFFIVGVGVDKDDENELGVLEFIHCLVETLDKYFQSVCELDIMFNLEKTHFILDEMVLNGYICEGNRENVLKPLLLMDKVAHGDAGFGSK 462
            MIKFVLMVNK GQTR++SY+ W PI ERVALEAE++RRC SR+E QCSF E+RG+K++YRRYASLFF+V V  D ++ENEL +LEFIH LVET+DKYF+SVCELDIMF++EK H+I+DEMV+NG I E N+ N+LKPL LMDK+A  + G  S+
Sbjct:    1 MIKFVLMVNKQGQTRLASYFQWYPIQERVALEAEIIRRCLSRTEFQCSFIEYRGFKVVYRRYASLFFVVAV--DGEEENELAILEFIHSLVETMDKYFESVCELDIMFHIEKAHYIVDEMVVNGSIVENNKVNILKPLALMDKLASAEGGIFSR 152          
BLAST of mRNA_M-pyrifera_M_contig727.17269.1 vs. uniprot
Match: A0A2R5GZ26_9STRA (AP complex subunit sigma n=1 Tax=Hondaea fermentalgiana TaxID=2315210 RepID=A0A2R5GZ26_9STRA)

HSP 1 Score: 222 bits (566), Expect = 2.870e-72
Identity = 104/145 (71.72%), Postives = 127/145 (87.59%), Query Frame = 1
Query:    1 MIKFVLMVNKMGQTRVSSYYDWMPIPERVALEAEVVRRCFSRSELQCSFFEFRGYKIIYRRYASLFFIVGVGVDKDDENELGVLEFIHCLVETLDKYFQSVCELDIMFNLEKTHFILDEMVLNGYICEGNRENVLKPLLLMDKVA 435
            MIKFVLMVNK GQTR+SSYY+W+ I ER ALEAE++R+C +R+E QCSF E RGYK++YRRYASLFFIVGV  D+D+ENELG+LEFIHCLVETLDKYF +VCELDIMFNLEK HFIL+EMV NG++ E N+ NVLKP+ ++D ++
Sbjct:    1 MIKFVLMVNKQGQTRLSSYYEWLTIEERGALEAEIIRKCLARTENQCSFMENRGYKVVYRRYASLFFIVGV--DQDEENELGILEFIHCLVETLDKYFSNVCELDIMFNLEKAHFILNEMVQNGHVVEANKANVLKPITMLDSIS 143          
BLAST of mRNA_M-pyrifera_M_contig727.17269.1 vs. uniprot
Match: F0W0A7_9STRA (AP complex subunit sigma n=21 Tax=Oomycota TaxID=4762 RepID=F0W0A7_9STRA)

HSP 1 Score: 218 bits (554), Expect = 1.930e-70
Identity = 106/143 (74.13%), Postives = 125/143 (87.41%), Query Frame = 1
Query:    1 MIKFVLMVNKMGQTRVSSYYDWMPIPERVALEAEVVRRCFSRSELQCSFFEFRGYKIIYRRYASLFFIVGVGVDKDDENELGVLEFIHCLVETLDKYFQSVCELDIMFNLEKTHFILDEMVLNGYICEGNRENVLKPLLLMDK 429
            MIKFVLMVNK GQTR++ YYD++ I ERVALEAE++R+C SR+E QCSF E+RGYK+IYRRYASLFFIVGV  D D ENELG+LEFIH LVET+DKYF+SVCELDIMFNLEK HFILDEMV+NG I E N+ ++LKP+ LM+K
Sbjct:    1 MIKFVLMVNKQGQTRLAQYYDFLSIQERVALEAEIIRKCLSRNENQCSFVEYRGYKVIYRRYASLFFIVGVQND-DSENELGILEFIHALVETMDKYFESVCELDIMFNLEKAHFILDEMVMNGSIVESNKVSILKPIHLMEK 142          
BLAST of mRNA_M-pyrifera_M_contig727.17269.1 vs. uniprot
Match: W7U353_9STRA (AP complex subunit sigma n=2 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7U353_9STRA)

HSP 1 Score: 217 bits (552), Expect = 8.590e-70
Identity = 106/166 (63.86%), Postives = 128/166 (77.11%), Query Frame = 1
Query:    1 MIKFVLMVNKMGQTRVSSYYDWMPIPERVALEAEVVRRCFSRSELQCSFFEFRGYKIIYRRYASLFFIVGVGVDKDD------------ENELGVLEFIHCLVETLDKYFQSVCELDIMFNLEKTHFILDEMVLNGYICEGNRENVLKPLLLMDKVAHGDAGFGSK 462
            MI+F+L VNK GQTR+SSYY WM IPERVALEAE++R+C SRSELQC F E+RG+K+IYRRYASLFFIVGV  D               ENEL +LEFIH LVETLDKYF+SVCELDIMFNLEK HFI+DEMV+NG+I + N+ N+LKP+ LM+K +  +    S+
Sbjct:    1 MIRFILFVNKQGQTRLSSYYTWMSIPERVALEAEIIRKCLSRSELQCQFIEYRGHKVIYRRYASLFFIVGVEGDDATGSAHSVSTNGGCENELAILEFIHALVETLDKYFESVCELDIMFNLEKAHFIVDEMVINGHIVDANKTNILKPIHLMEKASASEESIFSR 166          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig727.17269.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LPP6_ECTSI3.580e-10395.54AP complex subunit sigma n=1 Tax=Ectocarpus silicu... [more]
A0A835Z9B6_9STRA1.400e-9388.46AP complex subunit sigma n=1 Tax=Tribonema minus T... [more]
A0A7S2UUI5_9STRA8.640e-7872.73AP complex subunit sigma n=1 Tax=Fibrocapsa japoni... [more]
F0Y567_AURAN5.560e-7472.92AP complex subunit sigma n=1 Tax=Aureococcus anoph... [more]
A0A7S3ZZG7_9STRA3.100e-7373.61AP complex subunit sigma n=1 Tax=Pelagomonas calce... [more]
A0A7S2FA09_9STRA8.850e-7372.08AP complex subunit sigma n=1 Tax=Florenciella parv... [more]
A0A7S3GXI4_9STRA2.630e-7267.53AP complex subunit sigma n=1 Tax=Spumella elongata... [more]
A0A2R5GZ26_9STRA2.870e-7271.72AP complex subunit sigma n=1 Tax=Hondaea fermental... [more]
F0W0A7_9STRA1.930e-7074.13AP complex subunit sigma n=21 Tax=Oomycota TaxID=4... [more]
W7U353_9STRA8.590e-7063.86AP complex subunit sigma n=2 Tax=Nannochloropsis g... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig727contigM-pyrifera_M_contig727:30587..31937 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score302.4
Seed ortholog evalue1.3e-79
Seed eggNOG ortholog2880.D8LPP6
KEGG rclassRC00004,RC00041
KEGG koko:K00649,ko:K12403
KEGG ReactionR01013
KEGG Pathwayko00564,ko04142,ko04146,map00564,map04142,map04146
Hectar predicted targeting categoryother localisation
EggNOG free text desc.protein transport
EggNOG OGsCOG5030@1,KOG0934@2759
Ec32 ortholog descriptionClathrin assembly complex, small subunit
Ec32 orthologEc-18_001980.1
EC2.3.1.42
COG Functional cat.U
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko04131
Exons3
Model size471
Cds size471
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig727.17269.1prot_M-pyrifera_M_contig727.17269.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig727 30587..31937 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816623.7903447-CDS-M-pyrifera_M_contig727:30586..307691622816623.7903447-CDS-M-pyrifera_M_contig727:30586..30769Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig727 30587..30769 +
1692278083.4462917-CDS-M-pyrifera_M_contig727:30586..307691692278083.4462917-CDS-M-pyrifera_M_contig727:30586..30769Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig727 30587..30769 +
1622816623.801976-CDS-M-pyrifera_M_contig727:31278..313831622816623.801976-CDS-M-pyrifera_M_contig727:31278..31383Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig727 31279..31383 +
1692278083.461946-CDS-M-pyrifera_M_contig727:31278..313831692278083.461946-CDS-M-pyrifera_M_contig727:31278..31383Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig727 31279..31383 +
1622816623.8126698-CDS-M-pyrifera_M_contig727:31754..319371622816623.8126698-CDS-M-pyrifera_M_contig727:31754..31937Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig727 31755..31937 +
1692278083.4740117-CDS-M-pyrifera_M_contig727:31754..319371692278083.4740117-CDS-M-pyrifera_M_contig727:31754..31937Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig727 31755..31937 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig727.17269.1

>prot_M-pyrifera_M_contig727.17269.1 ID=prot_M-pyrifera_M_contig727.17269.1|Name=mRNA_M-pyrifera_M_contig727.17269.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=157bp
MIKFVLMVNKMGQTRVSSYYDWMPIPERVALEAEVVRRCFSRSELQCSFF
EFRGYKIIYRRYASLFFIVGVGVDKDDENELGVLEFIHCLVETLDKYFQS
VCELDIMFNLEKTHFILDEMVLNGYICEGNRENVLKPLLLMDKVAHGDAG
FGSKSS*
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mRNA from alignment at M-pyrifera_M_contig727:30587..31937+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig727.17269.1 ID=mRNA_M-pyrifera_M_contig727.17269.1|Name=mRNA_M-pyrifera_M_contig727.17269.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=1351bp|location=Sequence derived from alignment at M-pyrifera_M_contig727:30587..31937+ (Macrocystis pyrifera P11B4 male)
ATGATCAAGTTTGTTCTGATGGTGAACAAAATGGGCCAGACACGAGTATC GTCATATTACGACTGGATGCCCATTCCGGAGAGGGTGGCGCTGGAAGCAG AGGTCGTTCGACGTTGCTTCTCGCGATCAGAGCTGCAGTGCTCGTTTTTC GAGTTTCGGGGCTACAAAATTATTTATCGACGGGTGAGCGGCAGAATTAA AGCGGGACTGGCAGGTCGACGAAACAGCGGCGCTTAACGACTTTGAACTC GCGGCGTGGATGTTTCGAACTGTGTAGTTTCGTAGTGTTGTTCCATTGAT GGCACCAAAACATTCCTTGACCGAGTTACATCTACCACGTATATATGGGG ATTGGGTGCGGTTTTGTTGCGTGATATTTCTTTGCGCACGGCTCCCATTG GAAGTTGTGGGGGTTGGTCATCTGTCCTTTGCTTTTCGAATGAAGTTGTT CCTGGACTCAGCGGGTATGGCGCTTGTCGGGGGCAATCTGGGGGGCTGGA CATACTGTACTGTTCTTGATTTGAGCGGTGAAGGTTGAGCGGGAACACCG CAGCACGCTTAGTTAAAAAAAACGTGTTACATACATGAGATAAGTTCAAG TAGAAGGTCCAATGTCTTGACCCAAGTTATCGCCGTGCCTATCTTCGATG TGTTCGGCAAAATTGTCGGATTGACTTTCCTCCACTCTCCAGTATGCTTC GCTGTTCTTCATTGTTGGCGTGGGGGTGGACAAGGACGACGAGAACGAGC TTGGCGTTCTGGAATTCATCCATTGCCTAGTGGAGACCTTAGATAAGGTA TGGCTCGTACACGCTTGCGACAAGAAAATGGTCCGACTGCTGTAGACTGG CCATCAAGAACAGCGTTGCAGCGAAAAATAAGAGTACCAGTCATCTAAAA AAAAATCGGCGTTTCCCTCCTGTGGGTCAACTGCCGAAGCTTTCTATACC TACACGTACAAACTGTCCCACCATCTGTTCAAACCAAAACTCGCAAATCA CGGCAATATAAATATCTCATGTTTCGTGTCTTTCCAGCATCCAGCAGTCG CTACAAGGTAGACGATGGACAACCCACGGTAGGCGCGTTGATACAGCCAT CAAAACGAAGCATCGGCAAACCATAAACGTAACCCGTCGCGCGAAAATGT CTGAATGTTGCTCCACAGTACTTCCAAAGCGTATGCGAGCTCGACATCAT GTTCAACCTGGAAAAAACGCACTTCATCCTCGACGAAATGGTCCTGAATG GATACATTTGCGAAGGAAACCGGGAGAACGTGCTAAAACCACTGCTATTG ATGGACAAGGTGGCACATGGCGATGCCGGCTTTGGCAGCAAGTCGTCATA A
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig727:30587..31937+

>mRNA_M-pyrifera_M_contig727.17269.1 ID=mRNA_M-pyrifera_M_contig727.17269.1|Name=mRNA_M-pyrifera_M_contig727.17269.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=942bp|location=Sequence derived from alignment at M-pyrifera_M_contig727:30587..31937+ (Macrocystis pyrifera P11B4 male)
ATGATCAAGTTTGTTCTGATGGTGAACAAAATGGGCCAGACACGAGTATC
GTCATATTACGACTGGATGCCCATTCCGGAGAGGGTGGCGCTGGAAGCAG
AGGTCGTTCGACGTTGCTTCTCGCGATCAGAGCTGCAGTGCTCGTTTTTC
GAGTTTCGGGGCTACAAAATTATTTATCGACGGATGATCAAGTTTGTTCT
GATGGTGAACAAAATGGGCCAGACACGAGTATCGTCATATTACGACTGGA
TGCCCATTCCGGAGAGGGTGGCGCTGGAAGCAGAGGTCGTTCGACGTTGC
TTCTCGCGATCAGAGCTGCAGTGCTCGTTTTTCGAGTTTCGGGGCTACAA
AATTATTTATCGACGGTATGCTTCGCTGTTCTTCATTGTTGGCGTGGGGG
TGGACAAGGACGACGAGAACGAGCTTGGCGTTCTGGAATTCATCCATTGC
CTAGTGGAGACCTTAGATAAGTATGCTTCGCTGTTCTTCATTGTTGGCGT
GGGGGTGGACAAGGACGACGAGAACGAGCTTGGCGTTCTGGAATTCATCC
ATTGCCTAGTGGAGACCTTAGATAAGTACTTCCAAAGCGTATGCGAGCTC
GACATCATGTTCAACCTGGAAAAAACGCACTTCATCCTCGACGAAATGGT
CCTGAATGGATACATTTGCGAAGGAAACCGGGAGAACGTGCTAAAACCAC
TGCTATTGATGGACAAGGTGGCACATGGCGATGCCGGCTTTGGCAGCAAG
TCGTCATAATACTTCCAAAGCGTATGCGAGCTCGACATCATGTTCAACCT
GGAAAAAACGCACTTCATCCTCGACGAAATGGTCCTGAATGGATACATTT
GCGAAGGAAACCGGGAGAACGTGCTAAAACCACTGCTATTGATGGACAAG
GTGGCACATGGCGATGCCGGCTTTGGCAGCAAGTCGTCATAA
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