mRNA_M-pyrifera_M_contig72471.17231.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig72471.17231.1
Unique NamemRNA_M-pyrifera_M_contig72471.17231.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig72471.17231.1 vs. uniprot
Match: A0A5J4U0R1_9EUKA (Mediator of RNA polymerase II transcription subunit 6 n=1 Tax=Streblomastix strix TaxID=222440 RepID=A0A5J4U0R1_9EUKA)

HSP 1 Score: 75.1 bits (183), Expect = 5.400e-14
Identity = 46/116 (39.66%), Postives = 66/116 (56.90%), Query Frame = 1
Query:    1 LTADTVLGYFSRSDFYDKECINERAAARGLTSEAARKLMTGFEYSVHPLAPGDAEAKSGETLFIVERWYWSGEGRPPELQAHYYILG--GRIFQGPDLHTVLAARLRRCSYFVREA 342
            LT D  L YFS S FYD+ CIN++A  + L +E AR  + G EYS+  L+  D        +FI+ +       +  +L A YYIL   GR++Q PDL++++ +RL R  YF R A
Sbjct:   27 LTRDNWLEYFSLSQFYDRNCINQKAREQQL-NEVARLNLIGIEYSLEDLSTDD--------IFIISKRNRDAP-KHTQLLALYYILAKEGRLYQCPDLNSIIKSRLNRALYFFRGA 132          
BLAST of mRNA_M-pyrifera_M_contig72471.17231.1 vs. uniprot
Match: A0A485KG13_9STRA (Mediator of RNA polymerase II transcription subunit 6 n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485KG13_9STRA)

HSP 1 Score: 75.5 bits (184), Expect = 6.080e-14
Identity = 46/132 (34.85%), Postives = 71/132 (53.79%), Query Frame = 1
Query:    1 LTADTVLGYFSRSDFYDKECINERAAARGLTSEAARKLMTGFEYSVHPLAPGDAEAKSGETLFIVERWYWSGEGRPPELQAHYYILGGRIFQGPDLHTVLAARLRRCSYFVREAVKAIKEDATWTPEGEATW 396
            L   TVL YF+ S +YDK C NE    +    E     MTG EY    L P DA+ +  + LF++ +   +G  R   + A YY+L   I+Q P+ +T+L +RL++CSY + +A KA+     ++P    +W
Sbjct:   28 LNHQTVLYYFAGSPWYDKSCNNEILKMQRRPLEHLVS-MTGIEYE---LQPNDAK-QLPQNLFVIHKKNRTGP-RTAAVMAIYYVLDQTIYQAPNTYTLLTSRLKKCSYRINKAFKALSAGVRFSPTEGYSW 153          
BLAST of mRNA_M-pyrifera_M_contig72471.17231.1 vs. uniprot
Match: A0A024TZS3_9STRA (Mediator of RNA polymerase II transcription subunit 6 n=2 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024TZS3_9STRA)

HSP 1 Score: 72.4 bits (176), Expect = 9.910e-13
Identity = 44/132 (33.33%), Postives = 71/132 (53.79%), Query Frame = 1
Query:    1 LTADTVLGYFSRSDFYDKECINERAAARGLTSEAARKLMTGFEYSVHPLAPGDAEAKSGETLFIVERWYWSGEGRPPELQAHYYILGGRIFQGPDLHTVLAARLRRCSYFVREAVKAIKEDATWTPEGEATW 396
            L   +VL YF+ S +YDK C NE    +    E  +  MTG EY    L P DA+ +  + L+++ +   +G  R   + A YY+L   I+Q P+ +T+L +RL++CS+ V +A KA+     ++P     W
Sbjct:   33 LDHQSVLYYFAGSPWYDKNCNNEILKMQRRPLEHLQG-MTGIEYE---LQPNDAK-QVPQILYVIHKKNRTGP-RTAHVMAVYYVLDATIYQAPNTYTLLTSRLKKCSFRVNKAFKALSAGVRFSPTEGYAW 158          
BLAST of mRNA_M-pyrifera_M_contig72471.17231.1 vs. uniprot
Match: A0A6G0XIG6_9STRA (Mediator of RNA polymerase II transcription subunit 6 n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0XIG6_9STRA)

HSP 1 Score: 71.2 bits (173), Expect = 2.190e-12
Identity = 45/133 (33.83%), Postives = 69/133 (51.88%), Query Frame = 1
Query:    1 LTADTVLGYFSRSDFYDKECINERAAARGLTSEAARKLMTGFEYSVHPLAPGDAEAKS-GETLFIVERWYWSGEGRPPELQAHYYILGGRIFQGPDLHTVLAARLRRCSYFVREAVKAIKEDATWTPEGEATW 396
            L   TVL YF+ S +YDK C NE    +    E     MTG EY +        EAK   + LF++ +   +G  R   + A YY+L   I+Q P+ +T+L +RL++CSY + +A KA+     ++P    +W
Sbjct:   28 LNHQTVLYYFAGSPWYDKSCNNEILKMQRRPLEHLVS-MTGIEYELQL-----NEAKQLPQNLFVIHKKNRTGP-RTASVMAIYYVLDQTIYQAPNTYTLLTSRLKKCSYRINKAFKALSAGVRFSPTEGYSW 153          
BLAST of mRNA_M-pyrifera_M_contig72471.17231.1 vs. uniprot
Match: W4GBN3_9STRA (Mediator of RNA polymerase II transcription subunit 6 n=1 Tax=Aphanomyces astaci TaxID=112090 RepID=W4GBN3_9STRA)

HSP 1 Score: 69.7 bits (169), Expect = 6.580e-12
Identity = 43/133 (32.33%), Postives = 69/133 (51.88%), Query Frame = 1
Query:    1 LTADTVLGYFSRSDFYDKECINERAAARGLTSEAARKLMTGFEYSVHPLAPGDAEAKS-GETLFIVERWYWSGEGRPPELQAHYYILGGRIFQGPDLHTVLAARLRRCSYFVREAVKAIKEDATWTPEGEATW 396
            L   +VL YF+ S +YDK C NE    +    E  +  MTG EY + P      EAK   + L+++ +   +   R   + A YY+L   I+Q P+ +T+L +RL++CSY + +A KA+     ++P     W
Sbjct:   30 LDHQSVLYYFAGSPWYDKSCNNEILKMQRRPLEHLQG-MTGIEYELQP-----NEAKQLPQVLYVIHKKNRTSP-RTANVMAVYYVLDCTIYQAPNTYTLLTSRLKKCSYRINKAFKALSAGVRFSPTEGYAW 155          
BLAST of mRNA_M-pyrifera_M_contig72471.17231.1 vs. uniprot
Match: W4GAK2_9STRA (Mediator of RNA polymerase II transcription subunit 6 n=6 Tax=Aphanomyces astaci TaxID=112090 RepID=W4GAK2_9STRA)

HSP 1 Score: 69.7 bits (169), Expect = 9.460e-12
Identity = 43/133 (32.33%), Postives = 69/133 (51.88%), Query Frame = 1
Query:    1 LTADTVLGYFSRSDFYDKECINERAAARGLTSEAARKLMTGFEYSVHPLAPGDAEAKS-GETLFIVERWYWSGEGRPPELQAHYYILGGRIFQGPDLHTVLAARLRRCSYFVREAVKAIKEDATWTPEGEATW 396
            L   +VL YF+ S +YDK C NE    +    E  +  MTG EY + P      EAK   + L+++ +   +   R   + A YY+L   I+Q P+ +T+L +RL++CSY + +A KA+     ++P     W
Sbjct:   30 LDHQSVLYYFAGSPWYDKSCNNEILKMQRRPLEHLQG-MTGIEYELQP-----NEAKQLPQVLYVIHKKNRTSP-RTANVMAVYYVLDCTIYQAPNTYTLLTSRLKKCSYRINKAFKALSAGVRFSPTEGYAW 155          
BLAST of mRNA_M-pyrifera_M_contig72471.17231.1 vs. uniprot
Match: UPI000644C0CC (hypothetical protein n=1 Tax=Acytostelium subglobosum LB1 TaxID=1410327 RepID=UPI000644C0CC)

HSP 1 Score: 61.6 bits (148), Expect = 7.860e-9
Identity = 41/126 (32.54%), Postives = 59/126 (46.83%), Query Frame = 1
Query:    1 LTADTVLGYFSRSDFYDKECINERAAARGLTSEAARKLMTGFEYSVHPLAPGDAEAKSGETLFIVERWYWSGEGRPPE--LQAHYYILGGRIFQGPDLHTVLAARLRRCSYFVREAVKAIKEDATW 372
            L   TVL YFS S FYDK+C NE    + L   +A K M G EY V               LF+V +        P +  +Q  YYI+ G I+Q PD+ +V  +R+ +  + +  A + +    TW
Sbjct:   44 LNKQTVLLYFSMSQFYDKQCNNEILKMQRL-DPSALKTMIGIEYEV--------VMAQEPMLFLVAK---QSRVSPTDASVQTLYYIINGTIYQSPDIRSVFESRMVQSLFHLIHAFEKLLSTVTW 157          
BLAST of mRNA_M-pyrifera_M_contig72471.17231.1 vs. uniprot
Match: A0A5S6R3W3_TRIMR (Mediator of RNA polymerase II transcription subunit 6 n=1 Tax=Trichuris muris TaxID=70415 RepID=A0A5S6R3W3_TRIMR)

HSP 1 Score: 60.1 bits (144), Expect = 3.490e-8
Identity = 39/134 (29.10%), Postives = 65/134 (48.51%), Query Frame = 1
Query:    1 LTADTVLGYFSRS--DFYDKECINERAAARGLTSEAARKLMTGFEYSVHPLAPGDAEAKSGETLFIVERWYWSGEGRPPELQAHYYILGGRIFQGPDLHTVLAARLRRCSYFVREAVKAIKEDATWTPEGEATW 396
            + A  VL YF+ +   FYD  C+NE+   + L+ E   +  +G EY++           + E LF++ + Y         L+  YYI+GG ++Q PDL +VL +RL+     +R A + +K    + P     W
Sbjct:   26 INASNVLDYFTNTMNPFYDPNCLNEQIRMQRLSPEVLSR-SSGVEYAL---------LYACEPLFVIRKQYRQANQTITPLE-DYYIIGGTVYQAPDLCSVLNSRLQASINHLRTAFEEVKSYYRFHPAKGYYW 148          
BLAST of mRNA_M-pyrifera_M_contig72471.17231.1 vs. uniprot
Match: A0A1X7UXR5_AMPQE (Mediator of RNA polymerase II transcription subunit 6 n=2 Tax=Amphimedon queenslandica TaxID=400682 RepID=A0A1X7UXR5_AMPQE)

HSP 1 Score: 59.7 bits (143), Expect = 4.950e-8
Identity = 40/135 (29.63%), Postives = 67/135 (49.63%), Query Frame = 1
Query:    1 LTADTVLGYFSR--SDFYDKECINERAAA-RGLTSEAARKLMTGFEYSVHPLAPGDAEAKSGETLFIVERWYWSGEGRPPELQAHYYILGGRIFQGPDLHTVLAARLRRCSYFVREAVKAIKEDATWTPEGEATW 396
            L  D ++ YFS+  + FYD+ C NE     RG  S      MTG EYS+  +            L+++++ +     +   L A+YY+LGG ++Q PDLH+V+ +RL    + + +A +  +  A + P     W
Sbjct:   20 LNQDNIMDYFSQRSNPFYDRTCNNEVIRMQRGDPSHLVN--MTGIEYSLFLVQE--------PVLYVIKKVHRQSPEKVIPL-ANYYVLGGVVYQCPDLHSVINSRLLSSLHLIHQAFEEAQSYARYGPSQGYYW 143          
BLAST of mRNA_M-pyrifera_M_contig72471.17231.1 vs. uniprot
Match: MED6_DICDI (Putative mediator of RNA polymerase II transcription subunit 6 n=1 Tax=Dictyostelium discoideum TaxID=44689 RepID=MED6_DICDI)

HSP 1 Score: 59.3 bits (142), Expect = 7.990e-8
Identity = 38/124 (30.65%), Postives = 53/124 (42.74%), Query Frame = 1
Query:    1 LTADTVLGYFSRSDFYDKECINERAAARGLTSEAARKLMTGFEYSVHPLAPGDAEAKSGETLFIVERWYWSGEGRPPELQAHYYILGGRIFQGPDLHTVLAARLRRCSYFVREAVKAIKEDATW 372
            L   T+L YFS S FYDK C NE+   + L   A  K M G EY +          K  E  F +             +   YY++ G I+Q P+LH V  +R+ +    + EA  +I     W
Sbjct:   82 LNPQTILQYFSYSQFYDKNCNNEQLKMQRLDLSAL-KNMDGLEYEL---------IKFVEPSFFLIAKQTRISPTDVLINTLYYVINGNIYQAPELHVVFKSRVSQSISHLSEAFNSISSIVNW 195          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig72471.17231.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A5J4U0R1_9EUKA5.400e-1439.66Mediator of RNA polymerase II transcription subuni... [more]
A0A485KG13_9STRA6.080e-1434.85Mediator of RNA polymerase II transcription subuni... [more]
A0A024TZS3_9STRA9.910e-1333.33Mediator of RNA polymerase II transcription subuni... [more]
A0A6G0XIG6_9STRA2.190e-1233.83Mediator of RNA polymerase II transcription subuni... [more]
W4GBN3_9STRA6.580e-1232.33Mediator of RNA polymerase II transcription subuni... [more]
W4GAK2_9STRA9.460e-1232.33Mediator of RNA polymerase II transcription subuni... [more]
UPI000644C0CC7.860e-932.54hypothetical protein n=1 Tax=Acytostelium subglobo... [more]
A0A5S6R3W3_TRIMR3.490e-829.10Mediator of RNA polymerase II transcription subuni... [more]
A0A1X7UXR5_AMPQE4.950e-829.63Mediator of RNA polymerase II transcription subuni... [more]
MED6_DICDI7.990e-830.65Putative mediator of RNA polymerase II transcripti... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig72471contigM-pyrifera_M_contig72471:306..707 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score81.6
Seed ortholog evalue3e-13
Seed eggNOG ortholog4792.ETI42636
Preferred nameMED6
KEGG koko:K15128
Hectar predicted targeting categoryother localisation
GOsGO:0000151,GO:0000428,GO:0001128,GO:0003674,GO:0003676,GO:0003677,GO:0003712,GO:0003713,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005667,GO:0005737,GO:0006139,GO:0006351,GO:0006352,GO:0006355,GO:0006357,GO:0006366,GO:0006367,GO:0006464,GO:0006725,GO:0006807,GO:0007275,GO:0007399,GO:0007406,GO:0008134,GO:0008150,GO:0008152,GO:0008285,GO:0009058,GO:0009059,GO:0009889,GO:0009891,GO:0009893,GO:0009987,GO:0010467,GO:0010468,GO:0010556,GO:0010557,GO:0010604,GO:0010628,GO:0010721,GO:0016043,GO:0016070,GO:0016567,GO:0016591,GO:0016592,GO:0018130,GO:0019219,GO:0019222,GO:0019438,GO:0019538,GO:0019827,GO:0022008,GO:0022607,GO:0030154,GO:0030880,GO:0031323,GO:0031325,GO:0031326,GO:0031328,GO:0031974,GO:0031981,GO:0032446,GO:0032501,GO:0032502,GO:0032774,GO:0032991,GO:0034622,GO:0034641,GO:0034645,GO:0034654,GO:0036211,GO:0042127,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043412,GO:0043933,GO:0044085,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044271,GO:0044422,GO:0044424,GO:0044428,GO:0044446,GO:0044451,GO:0044464,GO:0044798,GO:0045595,GO:0045596,GO:0045893,GO:0045935,GO:0045944,GO:0046483,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048699,GO:0048731,GO:0048856,GO:0048869,GO:0050767,GO:0050768,GO:0050789,GO:0050793,GO:0050794,GO:0051093,GO:0051123,GO:0051171,GO:0051173,GO:0051239,GO:0051241,GO:0051252,GO:0051254,GO:0051960,GO:0051961,GO:0055029,GO:0060255,GO:0060284,GO:0061695,GO:0065003,GO:0065004,GO:0065007,GO:0070013,GO:0070647,GO:0070847,GO:0070897,GO:0071704,GO:0071824,GO:0071840,GO:0072091,GO:0080090,GO:0090304,GO:0090575,GO:0097159,GO:0097659,GO:0098727,GO:0140110,GO:1901360,GO:1901362,GO:1901363,GO:1901564,GO:1901576,GO:1902494,GO:1902680,GO:1902692,GO:1903506,GO:1903508,GO:1990234,GO:2000026,GO:2000112,GO:2000177,GO:2000178,GO:2000647,GO:2001141
EggNOG free text desc.MED6 mediator sub complex component
EggNOG OGs3QAKU@4776,COG5097@1,KOG3169@2759
COG Functional cat.K
Best tax levelPeronosporales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03021
Exons1
Model size402
Cds size402
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816621.5407646-CDS-M-pyrifera_M_contig72471:305..7071622816621.5407646-CDS-M-pyrifera_M_contig72471:305..707Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig72471 306..707 -
1692278082.0625203-CDS-M-pyrifera_M_contig72471:305..7071692278082.0625203-CDS-M-pyrifera_M_contig72471:305..707Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig72471 306..707 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig72471.17231.1prot_M-pyrifera_M_contig72471.17231.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig72471 306..707 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig72471.17231.1

>prot_M-pyrifera_M_contig72471.17231.1 ID=prot_M-pyrifera_M_contig72471.17231.1|Name=mRNA_M-pyrifera_M_contig72471.17231.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=134bp
LTADTVLGYFSRSDFYDKECINERAAARGLTSEAARKLMTGFEYSVHPLA
PGDAEAKSGETLFIVERWYWSGEGRPPELQAHYYILGGRIFQGPDLHTVL
AARLRRCSYFVREAVKAIKEDATWTPEGEATWSS
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mRNA from alignment at M-pyrifera_M_contig72471:306..707-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig72471.17231.1 ID=mRNA_M-pyrifera_M_contig72471.17231.1|Name=mRNA_M-pyrifera_M_contig72471.17231.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=402bp|location=Sequence derived from alignment at M-pyrifera_M_contig72471:306..707- (Macrocystis pyrifera P11B4 male)
TTGACTGCCGATACTGTGTTGGGATATTTTTCGAGGTCCGACTTTTATGA CAAAGAGTGTATAAACGAGAGAGCAGCAGCAAGAGGGCTCACTTCGGAGG CTGCCAGGAAGTTGATGACTGGTTTCGAATATTCCGTGCATCCTCTTGCA CCAGGAGATGCAGAAGCAAAGAGTGGAGAAACGTTGTTTATTGTTGAGAG GTGGTATTGGAGCGGAGAAGGGCGACCACCTGAATTACAAGCCCATTATT ACATTTTGGGAGGCAGGATCTTTCAAGGTCCTGACCTACATACAGTGCTT GCAGCACGCTTGAGACGTTGTTCCTACTTTGTCAGAGAAGCTGTGAAAGC CATCAAAGAAGACGCCACATGGACTCCAGAAGGAGAAGCCACTTGGTCCT CC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig72471:306..707-

>mRNA_M-pyrifera_M_contig72471.17231.1 ID=mRNA_M-pyrifera_M_contig72471.17231.1|Name=mRNA_M-pyrifera_M_contig72471.17231.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=804bp|location=Sequence derived from alignment at M-pyrifera_M_contig72471:306..707- (Macrocystis pyrifera P11B4 male)
TTGACTGCCGATACTGTGTTGGGATATTTTTCGAGGTCCGACTTTTATGA
CAAAGAGTGTATAAACGAGAGAGCAGCAGCAAGAGGGCTCACTTCGGAGG
CTGCCAGGAAGTTGATGACTGGTTTCGAATATTCCGTGCATCCTCTTGCA
CCAGGAGATGCAGAAGCAAAGAGTGGAGAAACGTTGTTTATTGTTGAGAG
GTGGTATTGGAGCGGAGAAGGGCGACCACCTGAATTACAAGCCCATTATT
ACATTTTGGGAGGCAGGATCTTTCAAGGTCCTGACCTACATACAGTGCTT
GCAGCACGCTTGAGACGTTGTTCCTACTTTGTCAGAGAAGCTGTGAAAGC
CATCAAAGAAGACGCCACATGGACTCCAGAAGGAGAAGCCACTTGGTCCT
CCTTGACTGCCGATACTGTGTTGGGATATTTTTCGAGGTCCGACTTTTAT
GACAAAGAGTGTATAAACGAGAGAGCAGCAGCAAGAGGGCTCACTTCGGA
GGCTGCCAGGAAGTTGATGACTGGTTTCGAATATTCCGTGCATCCTCTTG
CACCAGGAGATGCAGAAGCAAAGAGTGGAGAAACGTTGTTTATTGTTGAG
AGGTGGTATTGGAGCGGAGAAGGGCGACCACCTGAATTACAAGCCCATTA
TTACATTTTGGGAGGCAGGATCTTTCAAGGTCCTGACCTACATACAGTGC
TTGCAGCACGCTTGAGACGTTGTTCCTACTTTGTCAGAGAAGCTGTGAAA
GCCATCAAAGAAGACGCCACATGGACTCCAGAAGGAGAAGCCACTTGGTC
CTCC
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