mRNA_M-pyrifera_M_contig70771.16894.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig70771.16894.1
Unique NamemRNA_M-pyrifera_M_contig70771.16894.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig70771.16894.1 vs. uniprot
Match: A0A251MQR1_PRUPE (DYW_deaminase domain-containing protein n=7 Tax=Prunus TaxID=3754 RepID=A0A251MQR1_PRUPE)

HSP 1 Score: 62.0 bits (149), Expect = 4.650e-8
Identity = 50/156 (32.05%), Postives = 79/156 (50.64%), Query Frame = 1
Query:   16 AASVVGW---MREEGVEVDEDTVCSLLQAC---GVAKNAEAATAILTELRGVEGSDVVGSSFLWALVKSGEVKKAAQVFAEIVALDKVDVDATLATPLISGLGDVGDAEGALEVFRSLKRLGAEADVVAYTAAITACGKAGMVEMAQRLFSEMRSI 465
            AA  VG+   MR  G+  D+ TV SLL+AC   G+ K AE    ++T+  G+  +  V +  L    K G +  + +VF E+   DKV   A LA   + G G       A+E+F  + ++G E D V +T  ++AC  +G+V+  +  F  M  +
Sbjct:  253 AADGVGYFNLMRRAGINPDDGTVLSLLEACENLGLQKLAEGVHGLITKC-GLYANATVATGLLDLYAKLGRLNYSLKVFGEVNNPDKVAWTAMLAGNAVHGNG-----REAMELFEGMVKVGVEPDHVTFTHLLSACSHSGLVKEGKNYFDIMSQV 402          
BLAST of mRNA_M-pyrifera_M_contig70771.16894.1 vs. uniprot
Match: A0A067L0G7_JATCU (DYW_deaminase domain-containing protein n=1 Tax=Jatropha curcas TaxID=180498 RepID=A0A067L0G7_JATCU)

HSP 1 Score: 61.6 bits (148), Expect = 6.640e-8
Identity = 50/161 (31.06%), Postives = 78/161 (48.45%), Query Frame = 1
Query:    1 GELEQAASVVGWMREEGVEVDEDTVCSLLQACGVAKNAEAATAILTELRGVEGSDV---------VGSSFLWALVKSGEVKKAAQVFAEIVALDKVDVDATLATPLISGLGDVGDAEGALEVFRSLKRLGAEADVVAYTAAITACGKAGMVEMAQRLFSEM 456
            G+ E A  +   M +EGVE +E T  S++ AC V       TA + + +      +         V S+ +    K G+++ A++VF      D V  ++     ++SG    G    ALEVF+ ++RL  E D V +T  +TAC  AG+VE  QR F+ M
Sbjct:  485 GDTEGAVKIFIQMAKEGVEPNEYTFSSVINACAVP------TAAVEQGKPFHAWSIKSRFNDALCVSSALVTMYAKRGDIESASEVFKRQEVRDIVSWNS-----MVSGYAQHGHGTKALEVFKEMQRLKLELDDVTFTGVLTACTHAGLVEEGQRHFNIM 634          
BLAST of mRNA_M-pyrifera_M_contig70771.16894.1 vs. uniprot
Match: UPI0002C363F9 (pentatricopeptide repeat-containing protein At1g09190 n=1 Tax=Fragaria vesca subsp. vesca TaxID=101020 RepID=UPI0002C363F9)

HSP 1 Score: 61.2 bits (147), Expect = 8.020e-8
Identity = 50/174 (28.74%), Postives = 83/174 (47.70%), Query Frame = 1
Query:   13 QAASVVGWMREEGVEVDEDTVCSLLQACGVAKNAEAATAI--LTELRGVEGSDV-VGSSFLWALVKSGEVKKAAQVFAEIVALDKVDVDATLATPLISGLGDVGDAEGALEVFRSLKRLGAEADVVAYTAAITACGKAGMVEMAQRLFSEMRSIPEMDITVEAANATLHAIARS 525
            +A  V   MREEG E+DE TV S+L ACG     E    I   T+ +G+ G  V VG++ +    K G++  A  VF  +   + V  +  +     +G G++G     +E+F  +     + +   +  A+T C  AG+VE  + LF+ M  I  ++  +E     +  + RS
Sbjct:  223 EAVEVFNEMREEGFEIDEATVVSVLPACGQLGAVEVGKWIHLYTDSKGLIGEVVSVGNAVVSFYCKCGDLDAACSVFKGMARKNVVSWNTLILGYAFNGRGELG-----IELFEEMMNADEKPNEATFVGALTCCTHAGLVEKGRDLFASMTEIHGIEPKLEHYGCMVDVLGRS 391          
BLAST of mRNA_M-pyrifera_M_contig70771.16894.1 vs. uniprot
Match: A0A4Y1RY99_PRUDU (Tetratricopeptide repeat-like superfamily protein n=1 Tax=Prunus dulcis TaxID=3755 RepID=A0A4Y1RY99_PRUDU)

HSP 1 Score: 59.3 bits (142), Expect = 9.320e-8
Identity = 46/136 (33.82%), Postives = 70/136 (51.47%), Query Frame = 1
Query:   37 MREEGVEVDEDTVCSLLQAC------GVAKNAEAATAILTELRGVEGSDVVGSSFLWALVKSGEVKKAAQVFAEIVALDKVDVDATLATPLISGLGDVGDAEGALEVFRSLKRLGAEADVVAYTAAITACGKAGMV 426
            M ++GVEV++ TV S+L+AC      GV +        + E RG+     V S+ +    K G ++ A QVF +I     VD D    T +ISGL   G  + A+++FR ++  G + D    TA ++AC  AG V
Sbjct:    1 MVDDGVEVNDATVVSVLRACAETGALGVGRRVHG----VVEGRGIGLKANVNSALIDMYAKCGCIRSARQVFDDI-----VDKDVFAWTAMISGLASHGHCQDAIDLFRKMQGFGIKPDERTMTAVLSACRNAGQV 127          
BLAST of mRNA_M-pyrifera_M_contig70771.16894.1 vs. uniprot
Match: A0A1U8B5S9_NELNU (pentatricopeptide repeat-containing protein At3g26782, mitochondrial-like n=1 Tax=Nelumbo nucifera TaxID=4432 RepID=A0A1U8B5S9_NELNU)

HSP 1 Score: 60.1 bits (144), Expect = 2.110e-7
Identity = 49/163 (30.06%), Postives = 83/163 (50.92%), Query Frame = 1
Query:   10 EQAASVVGWMREEGVEVDEDTVCSLLQACG---VAKNAEAATAILTELRGVEGSDVVGSSFLWALVKSGEVKKAAQVFAEIVALDKVDVDATLATPLISGLGDVGDAEGALEVFRSLKR-LGAEADVVAYTAAITACGKAGMVEMAQRLFSEMRSI---PEMD 477
            E+A  +   MR  G++V   T  S+L ACG   + +      A++T++ G EG+  + SS +    K G + +A  +F + V  + +     L T +I+G    G     LE+F  L    GA  D++ +TA +TAC  AG+++ A   F++M+     PE+D
Sbjct:  280 EEALKLFVKMRGSGLDVTYHTFSSILNACGSLTIIQQGRQIHALITKM-GSEGNVFIASSLIDMYSKCGSIDEARCIFDQTVKRNNI-----LWTSMITGYAQSGRGAEGLELFELLVNGEGARPDLICFTAVLTACNHAGLLDRAIYYFNKMKDYGLSPELD 436          
BLAST of mRNA_M-pyrifera_M_contig70771.16894.1 vs. uniprot
Match: A0A6A6MQN7_HEVBR (Uncharacterized protein n=1 Tax=Hevea brasiliensis TaxID=3981 RepID=A0A6A6MQN7_HEVBR)

HSP 1 Score: 58.9 bits (141), Expect = 5.170e-7
Identity = 50/152 (32.89%), Postives = 77/152 (50.66%), Query Frame = 1
Query:   13 QAASVVGWMREEGVEVDEDTVCSLLQACGVAKNAEAATAILTELRGVEGSDVV--GSSFLWALVKSGEVKKAAQVFAEIVALDKVDVDATLATPLISGLGDVGDAEGALEVFRSLKRLGAEADVVAYTAAITACGKAGMVEMAQRLFSEMRS 462
            QA +++  M+ E VE DE T+ + L ACG     E    I + +   E S  V   ++ +    KSG +KKA +VF  +     +       T +ISGL   G    ALE+F  ++R   + + V + A ++AC  AG+V++ Q  FS MRS
Sbjct:  237 QAIAILRRMQLENVEPDEITMLAALSACGHLGALELGEWIHSYIDKHELSRTVPLNNALIDMYAKSGNIKKALKVFENMKRKSIITW-----TTMISGLALHGLGREALELFSCMERARVKPNDVTFIAILSACSHAGLVQIGQSFFSNMRS 383          
BLAST of mRNA_M-pyrifera_M_contig70771.16894.1 vs. uniprot
Match: UPI001CC4D885 (pentatricopeptide repeat-containing protein At1g08070, chloroplastic-like n=1 Tax=Telopea speciosissima TaxID=54955 RepID=UPI001CC4D885)

HSP 1 Score: 56.2 bits (134), Expect = 4.520e-6
Identity = 42/156 (26.92%), Postives = 77/156 (49.36%), Query Frame = 1
Query:    1 GELEQAASVVGWMREEGVEVDEDTVCSLLQACGVAKNAEAATAI--LTELRGVEGSDVVGSSFLWALVKSGEVKKAAQVFAEIVALDKVDVDATLATPLISGLGDVGDAEGALEVFRSLKRLGAEADVVAYTAAITACGKAGMVEMAQRLFSEMRS 462
            G   +A  +   MR E ++ ++ T+ S L +C    + E    I    E  G+  +  V ++ +    K G ++KA QVF +++  D V  ++     +I GL   G AE A+ ++  +K    + + + + + +TAC  AG+VE+  + F  MRS
Sbjct:  327 GRPNEALELFDLMRSENIQPNDVTLVSALSSCAQMGSVEDGERIGSYIESEGLTLNVYVCAALIDMYAKCGNIRKARQVFDQVIHRDIVSWNS-----IIGGLAINGFAEDAIALYDKMKETEVKPNDITFVSLLTACTHAGLVELGLKFFKSMRS 477          
BLAST of mRNA_M-pyrifera_M_contig70771.16894.1 vs. uniprot
Match: A0A6J1AC37_9ROSI (pentatricopeptide repeat-containing protein At5g40410, mitochondrial n=3 Tax=Byttnerioideae TaxID=214909 RepID=A0A6J1AC37_9ROSI)

HSP 1 Score: 55.8 bits (133), Expect = 6.100e-6
Identity = 42/158 (26.58%), Postives = 80/158 (50.63%), Query Frame = 1
Query:    1 GELEQAASVVGWMREEGVEVDEDTVCSLLQAC---GVAKNAEAATAILTELRGVEGSDVVGSSFLWALVKSGEVKKAAQVFAEIVALDKVDVDATLATPLISGLGDVGDAEGALEVFRSLKRLGAEADVVAYTAAITACGKAGMVEMAQRLFSEMRSI 465
            G  E++  +   MR  GVE D+ T+ ++LQAC   GV   A +   ++    G+  +  + ++ L    K G +  +++VF EI     +D D    T +++G    G  + A+++F+ + + G + D V +T  ++AC  +G+V   +R F  M  +
Sbjct:  233 GLAEESMGIFIMMRRAGVEFDQATMLTVLQACENLGVRNLAGSIHGLILSF-GITANVTIATALLNLYSKLGCLHASSKVFGEI-----IDPDNVAWTAMLAGYAVHGHGKDAIKLFQIMVKKGVQPDHVTFTHLLSACSHSGLVNEGKRYFKIMSEV 384          
BLAST of mRNA_M-pyrifera_M_contig70771.16894.1 vs. uniprot
Match: A0A2N9ENZ2_FAGSY (DYW_deaminase domain-containing protein n=5 Tax=Fagaceae TaxID=3503 RepID=A0A2N9ENZ2_FAGSY)

HSP 1 Score: 55.5 bits (132), Expect = 8.310e-6
Identity = 40/148 (27.03%), Postives = 79/148 (53.38%), Query Frame = 1
Query:   52 VEVDEDTVCSLLQACGVAKNAEAATAILTEL--RGVEGSDVVGSSFLWALVKSGEVKKAAQVFAEIVALDKVDVDATLATPLISGLGDVGDAEGALEVFRSLKRLGAEADVVAYTAAITACGKAGMVEMAQRLFSEMRSIPEMDITVE 489
            ++ D   + S L AC    + +    I T +   G+E   ++G   +   VK G++++A +VF +   L+K  + A   T +I+G    G  + AL+ F  ++  G + +++ +TA +TAC  AG+V+  + LF  M ++ +++ T+E
Sbjct:  266 IKPDNVALVSSLSACARLGSLDQGRWIHTYIDKNGIEIDPILGCVLIDMYVKCGDMEEALKVFRK---LEKKGISAW--TAIINGFAIHGQGQEALDWFMQMQEAGIKPNLITFTAILTACSHAGLVDEGKLLFKSMMTVYKLNPTIE 408          
BLAST of mRNA_M-pyrifera_M_contig70771.16894.1 vs. uniprot
Match: B8LQA8_PICSI (DYW_deaminase domain-containing protein n=1 Tax=Picea sitchensis TaxID=3332 RepID=B8LQA8_PICSI)

HSP 1 Score: 55.5 bits (132), Expect = 8.620e-6
Identity = 44/144 (30.56%), Postives = 68/144 (47.22%), Query Frame = 1
Query:   37 MREEGVEVDEDTVCSLLQACGVAKNAEAATAI--LTELRGVEGSDVVGSSFLWALVKSGEVKKAAQVFAEIVALDKVDVDATLATPLISGLGDVGDAEGALEVFRSLKRLGAEADVVAYTAAITACGKAGMVEMAQRLFSEMRS 462
            M+ +G++ D   + S+L AC      E    I   T   G E + VVG+  +    K G V  A ++F  +   D V       T +I   G  G  E AL +F  ++  G + D +A+TA +TAC  AG+V+   + F  M+S
Sbjct:  414 MQAQGIKPDSFAIVSVLPACAHFLALEQGKQIHGYTIRSGFESNVVVGTGLVDIYAKCGNVNTAQKLFERMPEQDVVSW-----TTMILAYGIHGHGEDALALFSKMQETGTKLDHIAFTAILTACSHAGLVDQGLQYFQCMKS 552          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig70771.16894.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 20
Match NameE-valueIdentityDescription
A0A251MQR1_PRUPE4.650e-832.05DYW_deaminase domain-containing protein n=7 Tax=Pr... [more]
A0A067L0G7_JATCU6.640e-831.06DYW_deaminase domain-containing protein n=1 Tax=Ja... [more]
UPI0002C363F98.020e-828.74pentatricopeptide repeat-containing protein At1g09... [more]
A0A4Y1RY99_PRUDU9.320e-833.82Tetratricopeptide repeat-like superfamily protein ... [more]
A0A1U8B5S9_NELNU2.110e-730.06pentatricopeptide repeat-containing protein At3g26... [more]
A0A6A6MQN7_HEVBR5.170e-732.89Uncharacterized protein n=1 Tax=Hevea brasiliensis... [more]
UPI001CC4D8854.520e-626.92pentatricopeptide repeat-containing protein At1g08... [more]
A0A6J1AC37_9ROSI6.100e-626.58pentatricopeptide repeat-containing protein At5g40... [more]
A0A2N9ENZ2_FAGSY8.310e-627.03DYW_deaminase domain-containing protein n=5 Tax=Fa... [more]
B8LQA8_PICSI8.620e-630.56DYW_deaminase domain-containing protein n=1 Tax=Pi... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig70771contigM-pyrifera_M_contig70771:31..600 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeViridiplantae
Seed ortholog score73.9
Seed ortholog evalue8.1e-11
Seed eggNOG ortholog3712.Bo7g114930.1
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003676,GO:0003723,GO:0003824,GO:0004518,GO:0004519,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0006139,GO:0006725,GO:0006807,GO:0008150,GO:0008152,GO:0009451,GO:0009987,GO:0016070,GO:0016071,GO:0016556,GO:0016787,GO:0016788,GO:0034641,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043412,GO:0044237,GO:0044238,GO:0044424,GO:0044464,GO:0046483,GO:0071704,GO:0090304,GO:0090305,GO:0097159,GO:1901360,GO:1901363
EggNOG free text desc.Pentatricopeptide repeat domain
EggNOG OGs37QXD@33090,3GHN5@35493,3HPC3@3699,KOG4197@1,KOG4197@2759
COG Functional cat.S
Best tax levelBrassicales
Best eggNOG OGNA|NA|NA
Exons2
Model size525
Cds size525
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816601.3940089-CDS-M-pyrifera_M_contig70771:30..3861622816601.3940089-CDS-M-pyrifera_M_contig70771:30..386Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig70771 31..386 -
1692278070.8302794-CDS-M-pyrifera_M_contig70771:30..3861692278070.8302794-CDS-M-pyrifera_M_contig70771:30..386Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig70771 31..386 -
1622816601.4079983-CDS-M-pyrifera_M_contig70771:431..6001622816601.4079983-CDS-M-pyrifera_M_contig70771:431..600Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig70771 432..600 -
1692278070.8407006-CDS-M-pyrifera_M_contig70771:431..6001692278070.8407006-CDS-M-pyrifera_M_contig70771:431..600Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig70771 432..600 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig70771.16894.1prot_M-pyrifera_M_contig70771.16894.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig70771 31..600 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig70771.16894.1

>prot_M-pyrifera_M_contig70771.16894.1 ID=prot_M-pyrifera_M_contig70771.16894.1|Name=mRNA_M-pyrifera_M_contig70771.16894.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=175bp
GELEQAASVVGWMREEGVEVDEDTVCSLLQACGVAKNAEAATAILTELRG
VEGSDVVGSSFLWALVKSGEVKKAAQVFAEIVALDKVDVDATLATPLISG
LGDVGDAEGALEVFRSLKRLGAEADVVAYTAAITACGKAGMVEMAQRLFS
EMRSIPEMDITVEAANATLHAIARS
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mRNA from alignment at M-pyrifera_M_contig70771:31..600-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig70771.16894.1 ID=mRNA_M-pyrifera_M_contig70771.16894.1|Name=mRNA_M-pyrifera_M_contig70771.16894.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=570bp|location=Sequence derived from alignment at M-pyrifera_M_contig70771:31..600- (Macrocystis pyrifera P11B4 male)
GGTGAATTAGAGCAAGCTGCTTCAGTAGTAGGTTGGATGCGAGAAGAAGG GGTTGAAGTGGATGAGGATACAGTTTGCTCGTTGTTGCAAGCTTGTGGTG TCGCGAAAAATGCAGAGGCAGCGACTGCGATACTTACAGAACTTCGTGGT GTTGAAGGGAGTGACGTGGGTGATGAGGTAAAGGACTTGGCTGCATGTGT GTCTTCTTCTGGAGTGGGTTCGAGCTTCTTGTGGGCTTTGGTAAAGAGTG GGGAAGTGAAGAAAGCTGCCCAGGTATTTGCTGAAATTGTTGCATTGGAC AAAGTTGATGTGGATGCAACACTGGCAACTCCGCTTATTTCAGGACTTGG GGATGTAGGAGATGCTGAAGGGGCGTTGGAAGTGTTTCGATCATTGAAAC GACTCGGCGCAGAAGCGGATGTTGTTGCTTATACAGCTGCAATTACCGCT TGTGGGAAAGCAGGTATGGTGGAGATGGCACAGCGTTTGTTTTCAGAAAT GCGCTCAATTCCTGAAATGGACATTACAGTTGAAGCTGCGAATGCAACAT TACACGCTATTGCAAGGTCT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig70771:31..600-

>mRNA_M-pyrifera_M_contig70771.16894.1 ID=mRNA_M-pyrifera_M_contig70771.16894.1|Name=mRNA_M-pyrifera_M_contig70771.16894.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1050bp|location=Sequence derived from alignment at M-pyrifera_M_contig70771:31..600- (Macrocystis pyrifera P11B4 male)
GGTGAATTAGAGCAAGCTGCTTCAGTAGTAGGTTGGATGCGAGAAGAAGG
GGTTGAAGTGGATGAGGATACAGTTTGCTCGTTGTTGCAAGCTTGTGGTG
TCGCGAAAAATGCAGAGGCAGCGACTGCGATACTTACAGAACTTCGTGGT
GTTGAAGGGAGTGACGTGGGGTGAATTAGAGCAAGCTGCTTCAGTAGTAG
GTTGGATGCGAGAAGAAGGGGTTGAAGTGGATGAGGATACAGTTTGCTCG
TTGTTGCAAGCTTGTGGTGTCGCGAAAAATGCAGAGGCAGCGACTGCGAT
ACTTACAGAACTTCGTGGTGTTGAAGGGAGTGACGTGGTGGGTTCGAGCT
TCTTGTGGGCTTTGGTAAAGAGTGGGGAAGTGAAGAAAGCTGCCCAGGTA
TTTGCTGAAATTGTTGCATTGGACAAAGTTGATGTGGATGCAACACTGGC
AACTCCGCTTATTTCAGGACTTGGGGATGTAGGAGATGCTGAAGGGGCGT
TGGAAGTGTTTCGATCATTGAAACGACTCGGCGCAGAAGCGGATGTTGTT
GCTTATACAGCTGCAATTACCGCTTGTGGGAAAGCAGGTATGGTGGAGAT
GGCACAGCGTTTGTTTTCAGAAATGCGCTCAATTCCTGAAATGGACATTA
CAGTTGAAGCTGCGAATGCAACATTACACGCTATTGCAAGGTCTTGGGTT
CGAGCTTCTTGTGGGCTTTGGTAAAGAGTGGGGAAGTGAAGAAAGCTGCC
CAGGTATTTGCTGAAATTGTTGCATTGGACAAAGTTGATGTGGATGCAAC
ACTGGCAACTCCGCTTATTTCAGGACTTGGGGATGTAGGAGATGCTGAAG
GGGCGTTGGAAGTGTTTCGATCATTGAAACGACTCGGCGCAGAAGCGGAT
GTTGTTGCTTATACAGCTGCAATTACCGCTTGTGGGAAAGCAGGTATGGT
GGAGATGGCACAGCGTTTGTTTTCAGAAATGCGCTCAATTCCTGAAATGG
ACATTACAGTTGAAGCTGCGAATGCAACATTACACGCTATTGCAAGGTCT
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