mRNA_M-pyrifera_M_contig7076.16889.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig7076.16889.1
Unique NamemRNA_M-pyrifera_M_contig7076.16889.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig7076.16889.1 vs. uniprot
Match: D7G7N6_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G7N6_ECTSI)

HSP 1 Score: 94.0 bits (232), Expect = 4.640e-23
Identity = 45/63 (71.43%), Postives = 53/63 (84.13%), Query Frame = 3
Query:   21 GVGSWLVDLIMKPGTSVQLVPAINVALVLIVGLLVYLVLNGESSIHVYVMGFLAVGLMASVNW 209
            G+G WLVDL MKPG+SVQLVP IN  L ++VGLLVY+  +GE SIHVYVMGFL++GLM SVNW
Sbjct:   23 GIGQWLVDLAMKPGSSVQLVPFINGVLCILVGLLVYVAASGEGSIHVYVMGFLSIGLMGSVNW 85          
BLAST of mRNA_M-pyrifera_M_contig7076.16889.1 vs. uniprot
Match: A0A7S1UA77_9STRA (Hypothetical protein n=1 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1UA77_9STRA)

HSP 1 Score: 75.9 bits (185), Expect = 1.960e-15
Identity = 37/80 (46.25%), Postives = 53/80 (66.25%), Query Frame = 3
Query:   27 GSWLVDLIMKPGTSVQLVPAINVALVLIVGLLVYLVL---NGESSIHVYVMGFLAVGLMASVNWFISEFNKVKQQQEQDG 257
            G W ++LIM PG+SVQL+P IN+AL +++G++ Y      NG   +H  VM FLA+GL   +NWF+ E+ K K +QE  G
Sbjct:   32 GEWAINLIMAPGSSVQLLPVINLALAIVIGVMGYTAYSTDNGAVQLHCSVMSFLALGLCVVINWFVGEYQKAKAEQEAAG 111          
BLAST of mRNA_M-pyrifera_M_contig7076.16889.1 vs. uniprot
Match: A0A7S2WUH3_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2WUH3_9STRA)

HSP 1 Score: 60.8 bits (146), Expect = 9.280e-10
Identity = 33/75 (44.00%), Postives = 48/75 (64.00%), Query Frame = 3
Query:   12 PSGGVGSWLVDLIMKPGTSVQLVPAINVALVLIVGLLVYLVLNGESSIHVYVMGFLAVGLMASVNWFISEFNKVK 236
            P  GV   LV  +++PGT   L+P IN +L  ++  L  +   G  SIH+ VM  LA+GLMASVNWF++EF +++
Sbjct:    6 PEDGVLQTLVVALVEPGTGHLLLPFINYSLCALLLTLAVVAAYGYGSIHLVVMAILALGLMASVNWFVAEFKRLE 80          
BLAST of mRNA_M-pyrifera_M_contig7076.16889.1 vs. uniprot
Match: A0A482S8M5_9ARCH (Uncharacterized protein n=1 Tax=archaeon TaxID=1906665 RepID=A0A482S8M5_9ARCH)

HSP 1 Score: 53.5 bits (127), Expect = 2.250e-7
Identity = 30/60 (50.00%), Postives = 43/60 (71.67%), Query Frame = 3
Query:   33 WLVDLIMKPGTSVQLVPAINVALVLIVG-LLVYLVLNGESSIHVYVMGFLAVGLMASVNW 209
            +++DLI +PG+S+ LVPAINV ++L++  LLV       + IH+ VM  LAVGL+ SVNW
Sbjct:    6 FILDLIFQPGSSLWLVPAINVTVLLLIAMLLVTGFYTDVAKIHIVVMSTLAVGLLLSVNW 65          
BLAST of mRNA_M-pyrifera_M_contig7076.16889.1 vs. uniprot
Match: L1JD47_GUITC (Uncharacterized protein n=1 Tax=Guillardia theta (strain CCMP2712) TaxID=905079 RepID=L1JD47_GUITC)

HSP 1 Score: 50.8 bits (120), Expect = 1.180e-5
Identity = 30/78 (38.46%), Postives = 48/78 (61.54%), Query Frame = 3
Query:   18 GGVGSWLVDLIMKPGTSV-----QLVPAINVALVLIVGLLVYLVLNGESSIHVYVMGFLAVGLMASVNWFISEFNKVK 236
            GG GSWLV+ +MKPG+S+     +++  + +AL L +  L++L      +IH Y+   LA+GL  SV +FI+E  K +
Sbjct:   11 GGAGSWLVEWLMKPGSSMSAGMQKVLNNVFIALFLCIIFLMWL--TNFGNIHTYIFFLLALGLFFSVRFFITELTKAQ 86          
BLAST of mRNA_M-pyrifera_M_contig7076.16889.1 vs. uniprot
Match: A0A1V9ZQU9_9STRA (Uncharacterized protein n=1 Tax=Achlya hypogyna TaxID=1202772 RepID=A0A1V9ZQU9_9STRA)

HSP 1 Score: 49.3 bits (116), Expect = 2.040e-5
Identity = 25/58 (43.10%), Postives = 39/58 (67.24%), Query Frame = 3
Query:   51 MKPGTSVQLVPAINVALVLIVGLLVYLVLNG-ESSIHVYVMGFLAVGLMASVNWFISE 221
            ++PG   + V  +N+ L+ +VG++   + +G E S H+Y+  FLAVGL AS+NWFI E
Sbjct:   12 LEPGAGNKFVIVLNLVLIALVGVISMTIWSGMEDSFHMYIFLFLAVGLTASINWFIVE 69          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig7076.16889.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 6
Match NameE-valueIdentityDescription
D7G7N6_ECTSI4.640e-2371.43Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A7S1UA77_9STRA1.960e-1546.25Hypothetical protein n=1 Tax=Phaeomonas parva TaxI... [more]
A0A7S2WUH3_9STRA9.280e-1044.00Hypothetical protein n=1 Tax=Rhizochromulina marin... [more]
A0A482S8M5_9ARCH2.250e-750.00Uncharacterized protein n=1 Tax=archaeon TaxID=190... [more]
L1JD47_GUITC1.180e-538.46Uncharacterized protein n=1 Tax=Guillardia theta (... [more]
A0A1V9ZQU9_9STRA2.040e-543.10Uncharacterized protein n=1 Tax=Achlya hypogyna Ta... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig7076contigM-pyrifera_M_contig7076:6336..11340 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Hectar predicted targeting categorysignal anchor
Ec32 ortholog descriptionexpressed unknown protein
Ec32 orthologEc-22_001650.1
Exons3
Model size323
Cds size309
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig7076.16889.1prot_M-pyrifera_M_contig7076.16889.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig7076 6338..11328 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816601.1355658-UTR-M-pyrifera_M_contig7076:6335..63371622816601.1355658-UTR-M-pyrifera_M_contig7076:6335..6337Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig7076 6336..6337 +
1692278070.6893563-UTR-M-pyrifera_M_contig7076:6335..63371692278070.6893563-UTR-M-pyrifera_M_contig7076:6335..6337Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig7076 6336..6337 +
1622816601.192224-UTR-M-pyrifera_M_contig7076:11328..113401622816601.192224-UTR-M-pyrifera_M_contig7076:11328..11340Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig7076 11329..11340 +
1692278070.7453399-UTR-M-pyrifera_M_contig7076:11328..113401692278070.7453399-UTR-M-pyrifera_M_contig7076:11328..11340Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig7076 11329..11340 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816601.1480906-CDS-M-pyrifera_M_contig7076:6337..64121622816601.1480906-CDS-M-pyrifera_M_contig7076:6337..6412Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig7076 6338..6412 +
1692278070.702796-CDS-M-pyrifera_M_contig7076:6337..64121692278070.702796-CDS-M-pyrifera_M_contig7076:6337..6412Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig7076 6338..6412 +
1622816601.1587968-CDS-M-pyrifera_M_contig7076:9195..93261622816601.1587968-CDS-M-pyrifera_M_contig7076:9195..9326Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig7076 9196..9326 +
1692278070.7225187-CDS-M-pyrifera_M_contig7076:9195..93261692278070.7225187-CDS-M-pyrifera_M_contig7076:9195..9326Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig7076 9196..9326 +
1622816601.179832-CDS-M-pyrifera_M_contig7076:11225..113281622816601.179832-CDS-M-pyrifera_M_contig7076:11225..11328Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig7076 11226..11328 +
1692278070.7335289-CDS-M-pyrifera_M_contig7076:11225..113281692278070.7335289-CDS-M-pyrifera_M_contig7076:11225..11328Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig7076 11226..11328 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig7076.16889.1

>prot_M-pyrifera_M_contig7076.16889.1 ID=prot_M-pyrifera_M_contig7076.16889.1|Name=mRNA_M-pyrifera_M_contig7076.16889.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=103bp
MPAPSGGVGSWLVDLIMKPGTSVQLVPAINVALVLIVGLLVYLVLNGESS
IHVYVMGFLAVGLMASVNWFISEFNKVKQQQEQDGGGAPAAKEDAAGRQK
SD*
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mRNA from alignment at M-pyrifera_M_contig7076:6336..11340+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig7076.16889.1 ID=mRNA_M-pyrifera_M_contig7076.16889.1|Name=mRNA_M-pyrifera_M_contig7076.16889.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=5005bp|location=Sequence derived from alignment at M-pyrifera_M_contig7076:6336..11340+ (Macrocystis pyrifera P11B4 male)
GCATGCCGGCACCTAGTGGAGGAGTAGGTTCATGGCTCGTCGACCTCATC ATGAAGCCCGGAACCTCTGTCCAATTGGTGAGCAAAGCAAGAGTATTTTC GCTCTACTGGTCTGTTTCGTGACTCACATATACCTCCTAGAGGTACCACA GCAATACTGGCAGCAGAGAAGCAAACAAGCAAGCGAACGGTCGTACTGCA CCGTCTCTGAGGCTTCAAGGCTTTCGAACACCCTGATGAGAGGCACACCC CTAGTACTAATAGGGGTGCCGAGACTCATGTTCTGCACATCGTGCGTGGT GTTACTTTAAGGGAGAGGAGGACGGCGCTATCTTCAAGCGTGATGAGGTT CGATTAACTGTCAAAGGGTCTGACTGAGGTCTGAGCGCACATCACCAAAG CGGCGGATTCTCAGGGTCGCGTTCGTTCCTACTGCCGTTGAACAACCATC TCACGACGCCCCGACCCAAGCATGCATGATGATACCTGGTACCAGTCTGA CAGCAAGGACGACGAACAGGCGCTGCTGTGTGTTACGTGTTTACCCGATC CCCGATACATGGGTTGCTCTCTTTGCCCCTACATAGTAGGACAGACTAAG GTGACACACTAAGGAAACTAAAGTAGGCTGCGTATAGAGGTTGTCCTACG AACAGCTGTTACCCGAATACGAGTATTAATATTACGGGGGGGCATAGTAA ATAGGACCAAATATTGATAGTAAAAATAGGTAAATATATAGGTTTTGTGT ATACCGTAGGTCCTATTTACTATGGACCCCCGTAATAGTATCTGTGAGAA TGGCCTGGACTCTGCTTGTGACAACGGGCAAACCGAACGAATGCTCAGCA GTGAAATCATGTACCGCCAGGTATAGGATCCATCGTAGATTGAGGAGTTC CATGTTGGATAGGGATTCTGGTAGTGGGATATCGGGATAATGTGCGAGCC TCCTGATGCCACTCATTGTTGAGCTCCTTATGAGACTGAGTCAACTCGAC AGCTCGAGAATAATACAGACAAGAGTACCGTGTACTTGGCTTGGGTGACC ACCACGCGTTCCCGTGCCACCTGTGTCTACAGCTTCAGAGATCGATCAAC ACACTATTAATAGTTAGCCGGTTATCTTCTATTGACGAGCGCGGCCATGG CCATCCCAACGGAAAATCCCAGAAGGGAGGGATCTGCGTCCAGCTAATGT CAAGTCACGAGGATGCCTGCATGGAGCAGGAAAGCGTGGAGTGTGTGTTG TAATATTTGAAGTTGGTCCATATTCAGAGAACGACGCAGAAGCTTCAGAG AGATGTAAAGCTGGCCAATGTTGAGATAACGACGCAGAAGCTTCAGAGAG AAGGTGTGAAGCTTATGCGTCGTTATCTGAGTATCGACCAGCTTTACGTG TTCTAACACACACTGCACGCTTTCCTCCTCCATGTCAGCAGCCCCGTGAC GTGATGTGAGCTGGACGCTGAACCCTCCCCTCTGGCATTCCCCTCTGGGA TGGCCGTGGCCGCGCTCGCCAATAGAAGATAACCGGATCACTATTAATAT TCAATAGTGTGTTGGAAAATCTCTAAAGCTGTACGTAGACGCAGCTGGCA CGGGAACGCGTGGCAGTGACTCAAGGCATGCTTGACTTCGACTGTCGGGT ACCATCAACGTTTCCAGGTGGAAAAAAGGAGAAAGTGGATTCCTCTATTC TCACCAGCAGTAGCGCGTGGCAAGGGGTTCGCCCCGAAAATTAATGTCGA GACCGAGTCGAACTTGCAAAGCAGATATTGTGTTGCTTGGAAGGGATACC TTGCTGTGGGTGCACGGGAATAAAAAGCGCAACCAACTAGCGCACTGAGT GAGTGGAGTAGTAGGGCATCCCTTCTCTGACCAATAAAGTCAATGCGTCG TTTTAGTTCGGATGCTTGTGGAATCCCCAGCAGCGCGAGCTGCTTTTGGT TGACCGTTGGTGTAATCGTCCTACGAAGTTCGCATGAGTTTTATCAATTC GGCATGTTGTCCCTTTGAGGCCGTTTCCTATGCGGTCCGATTTTTGAGCT TCAGATTTTGACCCGATCGCATTTTTAGATGTGAAGTTGGCAAATCGAAA CAGTCGTCCTTTGCGGAAAAATCAGATTTATTACTACGTGTGCTGAGATT CGAGCAAACGCCAAGACTAACTGCATTTGTTGTGGTCTGGCGCCACGAAG AGACGCGCTGTGTGCGCTGCCCTGCGCGTAGGACCCAAGCGTCCGAGCCA AGGCTGACTGCCTATGGTCTGGCAGCGAAGAGACGTGGCGCGTGCGCTAG AGTCAAGCTGTCGTGGTATTTTGTGTTTTAAAATGTGTCTGACTGCTGCT GCCGCTGTACAGCAGTGTAGTCTACGAGTACGTATCCATGGAGAAGTGCA CGTCGAGTGCGCTTGTCTTGGTCTCTAGCCCCTAGCCCGCGCCAACCGAC TGTCGTCTGACCACGGAGGGACGCGCCGACCGCGCGTCGGGCGCGAAGCA ACGTCGCTATACAGCTGTGTAACCATCCAACTCATCGATCGTTCTGCTGC TGTTTGCTGTCGTGGATAATGCCATTTTTCGATTTGAAAATCTGATGACC CAAGTCGTCGGATTTCGGGCCAAAATCCGACGACTTGGGTCATCGGATTC TCAAAAATCGAAACAATTCCGGTCGGATTATGCATATTTGGACTGCATAA GCAGGGTCATTCGTTTTCGAGATAAAAAAATATATAAATCTCAAGCTCGA GATCGAACCGCATATGAAACAGCCTGAGCTCCCAAAAAGGTACAGGACTT CTGAAATTGGTCGCTTTGCATTTCGGTTCGCGTGTCCCCCTTTTTGATGC GAACTCCCAGGTGCCGGCGATCAACGTGGCTCTGGTTCTCATCGTGGGTC TTCTGGTGTACCTGGTCTTGAACGGGGAGTCAAGCATCCACGTATACGTC ATGGGGTTTCTTGCCGTCGGTCTTATGGCGTCGGTAAATTGGTGAGCCCT CTGCGGACCATTGCAGTACTCGTACAGCACTCGTACAGCACAGACACAGT ATGCCATGCAGCGGGCGGAAGCGTATATACAAATATACGGGAGACGGAAC AAAGAGGGTACCAGGTACACCAGAAGCTGACGGGTGGCAACGTAAAATTA ACCGCAAAACAACGGAGTACGCCACGCCGAGGGCGGCCGTAAGGGAGACG AGACAGAGACTATTCGCCACAAGCCGACAAACTGAATTGCAGGACCGGAT AAAGTCAACTTACTCCACGAAGCTCAACTCGAACAAACCTAACACGCTAC CACTCTATCCGGAAATGTAGGGACCGTCTGGGTGCAAGTAGGAATGACCA AACTGTCCTTACATTTCCGAACAGAGCGATGTAAAGTAAGGAACAGGAGG CTGGGTGAGGCGGCTGAGTGTTTTGTGTGGAATGTTTTTTCAACGCTAAC GTCAGTATGACGTCTCCCGAATCTCGCTCAGGCTGGATTTCGAATCTTGG AACAGGTGCGCTCCACACTGCAGTACGCTCAGTACATAACAGCTCTCGAA TTCTCTGTTTTCTTGATAGTCTCAGCCTGGCCTCCGTAGTGTCCATGTTC TTATTTGTGCTCGTGTTCGTGTCAACCTTTGTGGGATCCAGATGTATCGC CCCGTGACGAGGCCAAGTGCGATCTTATCGATAGTTCAGTTGTCGGACGC GCATCAGCGTACGAGTAGCACCATGCTGTGTGTGCGTGCCGGGTATACCG AATGAGTAGTTGCGTGGCATCTGTCAGTTCTTCCTGATGTTTTAATCTCA GTCTTGAGACCTCTCAGTTGCAGCGACCCTTGGTCGCGTAAATGTCACTA TTTCGCAAAAAACAAAGGTCATATTTGTTTTGGCGCATTCAAAGCATTTT TTATTGTTTTATTTTCTTTCTTCCTCTCGTCTGTGTTTCTATACGCTTTT CTTTTCTTCGTTGGAAATGACTCTTACCAGTAACTTCTTTTGGACATCAA GCGTACAGTAGGGGGTGGGGGAGGTGCACTCATGTCATGCATGGCCGCAG TCGTACGACCGTAGACCCTCGCATCCCCACAATGCCGGGACGGAGCACGT CGGGTTTTCACCAACCAGGCAGGCACTGCTTGCACCAAGTGCGAAGCACC GCTAGCTGCTGTGCGAGCCGCGTGAAGGGTGAGCTGCATCCTACCAGGAA CCGCCTGTGAGGCGGACTTGCATACGGATGACAGCTTCGAATGAATTGAT TTGTTTTCTGGTGCGGCCCCTTCCAGTAGGGGGGTGCGTTTTCAGGGCCA ATTTGTTTAAACAGCCCGGGGCTGCTCGCGCTGCCGGTATAAAGCCGGCA GGAGAAACATGAGTAATACCACCCCCCCCCCCCCTGCCGTGGGTCGCGTC AGAGCTACACCCAGTTTTTTTTCTTCAAGCATTTAATGAAGCTTTTTTTG CACAAGTGGGATCGATGACTCTTAAGGGGTTAAGGTTAGGGCTCCCTACC GGCTATTTTCACAATAATACTGGGTATATATTACGGTGGGAAAAAATGAC CAGAACCAAATATGGTTTGTAAAAACAAGGAGATAACATGGTTTTAGTCT CCGTGATCGGTCTTATTATTACGGAGGGCCATAGTAAATAGGACCATGCA CAAAAACCTGTATATTTACCCATTTTTTCTAACAATATTTGGTCCTATTT ACCATGGTCCCCCGTAATACTATTGATACCACACTGGCCGGAAATATCAG GAAGACTTAGTCAGTAATATGAACACCAAGAGGTCCTTGTTTTTCAGGTC AGTTGTATGTGATACTATGCGCTCATGGAATAGTGAAAAGTGACCGACGA GCGCGGCTTCCCCTTGTCTGCCGGCACGATGACTCCCCAGGTTCATATCG GAGTTTAACAAGGTAAAGCAACAGCAAGAGCAGGACGGGGGAGGCGCACC TGCAGCAAAGGAGGACGCCGCAGGCCGACAAAAATCGGACTAGAAACGCC GGTGC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig7076:6336..11340+

>mRNA_M-pyrifera_M_contig7076.16889.1 ID=mRNA_M-pyrifera_M_contig7076.16889.1|Name=mRNA_M-pyrifera_M_contig7076.16889.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=618bp|location=Sequence derived from alignment at M-pyrifera_M_contig7076:6336..11340+ (Macrocystis pyrifera P11B4 male)
ATGCCGGCACCTAGTGGAGGAGTAGGTTCATGGCTCGTCGACCTCATCAT
GAAGCCCGGAACCTCTGTCCAATTGATGCCGGCACCTAGTGGAGGAGTAG
GTTCATGGCTCGTCGACCTCATCATGAAGCCCGGAACCTCTGTCCAATTG
GTGCCGGCGATCAACGTGGCTCTGGTTCTCATCGTGGGTCTTCTGGTGTA
CCTGGTCTTGAACGGGGAGTCAAGCATCCACGTATACGTCATGGGGTTTC
TTGCCGTCGGTCTTATGGCGTCGGTAAATTGGTGCCGGCGATCAACGTGG
CTCTGGTTCTCATCGTGGGTCTTCTGGTGTACCTGGTCTTGAACGGGGAG
TCAAGCATCCACGTATACGTCATGGGGTTTCTTGCCGTCGGTCTTATGGC
GTCGGTAAATTGGTTCATATCGGAGTTTAACAAGGTAAAGCAACAGCAAG
AGCAGGACGGGGGAGGCGCACCTGCAGCAAAGGAGGACGCCGCAGGCCGA
CAAAAATCGGACTAGGTTCATATCGGAGTTTAACAAGGTAAAGCAACAGC
AAGAGCAGGACGGGGGAGGCGCACCTGCAGCAAAGGAGGACGCCGCAGGC
CGACAAAAATCGGACTAG
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