mRNA_M-pyrifera_M_contig112020.2514.1 (mRNA) Macrocystis pyrifera P11B4 male

BLAST of mRNA_M-pyrifera_M_contig112020.2514.1 vs. uniprot
Match: L8GQC0_ACACA (RhoGEF domain containing protein n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8GQC0_ACACA)

HSP 1 Score: 109 bits (272), Expect = 2.670e-25
Identity = 50/117 (42.74%), Postives = 79/117 (67.52%), Query Frame = 1

Query:   16 VKLQALVRGHLARLRYAEISRHVAYRDKVAKEILATERSYVESLTTCLIVYMEPLKKTQRDSKPLLSPAQFKSIFSELPVLLNFNAELLGELEPLVEKWNAFQCIGPLFLKLVAFMK 366
            ++ Q+LVRG+L + RY  + R+VAYR +VA EIL TE  YV+S+  C+ V++ PL        PL++ A  ++IFS++ +L  FN+EL  E+E  V KW  ++C+G +FL+LV ++K
Sbjct:  199 IRFQSLVRGYLQKQRYRAMVRNVAYRSRVAHEILDTESFYVKSMNQCITVWLNPLNDAIASGSPLINAADVRAIFSDIALLYTFNSELYSEIESRVAKWGTYECLGDIFLRLVDYLK 315          

BLAST of mRNA_M-pyrifera_M_contig112020.2514.1 vs. uniprot
Match: L8GN17_ACACA (RhoGEF domain containing protein n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8GN17_ACACA)

HSP 1 Score: 102 bits (253), Expect = 9.990e-23
Identity = 50/120 (41.67%), Postives = 82/120 (68.33%), Query Frame = 1

Query:    7 KGVVKLQALVRGHLARLRYAEISRHVAYRDKVAKEILATERSYVESLTTCLIVYMEPLKKTQRDSKPLLSPAQFKSIFSELPVLLNFNAELLGELEPLVEKWNAFQCIGPLFLKLVAFMK 366
            +GVVKLQAL+RG LAR++Y +  R+ AYR+ VA+EI  TE+ YV++L     V++EPL+K        L    FK +F+++ V+ N+N ++L +++P + KW+  Q +G +FL++ AF+K
Sbjct:  332 RGVVKLQALLRGRLARVKYRKKLRNAAYRESVAREIEKTEQDYVDNLRLLSEVFLEPLRKELESKDEALEG--FKGVFADIQVIRNYNGKMLNDIKPRLAKWHPNQSLGDIFLQVAAFLK 449          

BLAST of mRNA_M-pyrifera_M_contig112020.2514.1 vs. uniprot
Match: L8HHL5_ACACA (RhoGEF domain containing protein n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8HHL5_ACACA)

HSP 1 Score: 97.4 bits (241), Expect = 3.940e-21
Identity = 55/129 (42.64%), Postives = 84/129 (65.12%), Query Frame = 1

Query:    7 KGVVKLQALVRGHLARLRYAEISRHVAYRDKVAKEILATERSYVESLTTCLIVYMEPLKKTQRDS--KPL-------LSPAQFKSIFSELPVLLNFNAELLGELEPLVEKWNAFQCIGPLFLKLVAFMK 366
            +GVV LQALVRG  AR  Y +  R VAYR+ VA+EIL TE+ YV +L   + V++ P+K++  D+  KP        ++  + KS++++L V++N+N +LL +L P VEKW   Q +G +FL+L  F+K
Sbjct:  177 RGVVLLQALVRGGRARKAYIKRVRDVAYRENVAREILQTEKDYVNNLMLLMSVFINPIKESLTDNVWKPKNGSKLGEVTLKEVKSLYNDLQVIVNYNGKLLRDLTPRVEKWTPHQKLGDIFLQLGLFLK 305          

BLAST of mRNA_M-pyrifera_M_contig112020.2514.1 vs. uniprot
Match: UPI000644FED1 (hypothetical protein n=1 Tax=Acytostelium subglobosum LB1 TaxID=1410327 RepID=UPI000644FED1)

HSP 1 Score: 92.8 bits (229), Expect = 1.700e-19
Identity = 47/122 (38.52%), Postives = 77/122 (63.11%), Query Frame = 1

Query:    4 EKGVVKLQALVRGHLARLRYAEISRHVAYRDKVAKEILATERSYVESLTTCLIVYMEPLKK-TQRDSKPLLSPAQFKSIFSELPVLLNFNAELLGELEPLVEKWNAFQCIGPLFLKLVAFMK 366
            EK  +K+QALVRGHLAR  + +  R+ AYR  +A E+LATE  Y++ L   ++ Y EP+++   R         +FK IFS + V+ N+N+ LL +L+P++E W+  + IG +F++ +  +K
Sbjct:  420 EKKWIKVQALVRGHLARRDFHKRVRNSAYRHNIADELLATEEMYIKHLDRLIVHYFEPIQEQATRIGIKATMLEEFKKIFSNIEVIRNYNSSLLADLKPVIENWSNSKTIGKIFIQFIFLLK 541          

BLAST of mRNA_M-pyrifera_M_contig112020.2514.1 vs. uniprot
Match: F1A1K8_DICPU (Uncharacterized protein (Fragment) n=1 Tax=Dictyostelium purpureum TaxID=5786 RepID=F1A1K8_DICPU)

HSP 1 Score: 88.6 bits (218), Expect = 5.110e-18
Identity = 50/123 (40.65%), Postives = 73/123 (59.35%), Query Frame = 1

Query:    4 EKGVVKLQALVRGHLARLRYAEISRHVAYRDKVAKEILATERSYVESLTTCLIVYMEPLKKTQRDSKPLLSPAQFKSIFSELPVLLNFNAELLGELEPLVE--KWNAFQCIGPLFLKLVAFMK 366
            E+G ++ QAL RG   R    +I R VAYR+KVA EIL TE  YV++L  C+  YMEPL      SK L++  Q K IFS++ ++ NF  + L +L+   +  KW  +Q +  +F+ + AF K
Sbjct:  212 ERGFIRFQALFRGFKTRQMVKKIRRDVAYREKVASEILKTEEDYVKNLNLCIKYYMEPLL-----SKELITKEQQKLIFSDISIIYNFGNKFLEQLKGRCQPGKWYVYQKLSDMFISISAFFK 329          

BLAST of mRNA_M-pyrifera_M_contig112020.2514.1 vs. uniprot
Match: A0A7S4L2J6_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Paramoeba aestuarina TaxID=180227 RepID=A0A7S4L2J6_9EUKA)

HSP 1 Score: 84.0 bits (206), Expect = 5.420e-18
Identity = 48/104 (46.15%), Postives = 66/104 (63.46%), Query Frame = 1

Query:    1 MEKGVVKLQALVRGHLARLRYAEISRHVAYRDKVAKEILATERSYVESLTTCLIVYMEPLKKTQRDSKPLLSPAQFKSIFSELPVLLNFNAELLGELEPLVEKW 312
            +EK   K QALVRG+LAR RY +I R+ AYR++V KE+L+TE +YV  L+  + V+M PL K     K  +SP    SIFS +P + + NA LL E+   V+ W
Sbjct:   68 IEKSCAKFQALVRGYLARKRYRQIIRNAAYRERVVKELLSTEETYVNDLSAAIDVFMIPLSKR---GKKDISP----SIFSTMPQIRDKNAALLSEIRDRVDHW 164          

BLAST of mRNA_M-pyrifera_M_contig112020.2514.1 vs. uniprot
Match: F1A4T1_DICPU (Uncharacterized protein n=1 Tax=Dictyostelium purpureum TaxID=5786 RepID=F1A4T1_DICPU)

HSP 1 Score: 87.8 bits (216), Expect = 9.560e-18
Identity = 46/121 (38.02%), Postives = 73/121 (60.33%), Query Frame = 1

Query:    4 EKGVVKLQALVRGHLARLRYAEISRHVAYRDKVAKEILATERSYVESLTTCLIVYMEPLKKTQRDSKPLLSPAQFKSIFSELPVLLNFNAELLGELEPLVEKWNAFQCIGPLFLKLVAFMK 366
            EK  +++Q+LVRGHLAR  Y +  R+ AYR  + KE+LATE+ Y+E+L   L  Y  P++  +  SK  +    F  + S L V++N+N  LL  ++P V+ W+  Q IG +F +   ++K
Sbjct:  287 EKKWIRIQSLVRGHLARRAYLKRVRYAAYRHNIVKELLATEKVYIENLEHLLSHYFNPMR--EESSKLNIKMDHFGLLISNLEVIINYNKNLLDIIKPKVDNWSHSQTIGDVFEQFTLYLK 405          

BLAST of mRNA_M-pyrifera_M_contig112020.2514.1 vs. uniprot
Match: A0A151Z724_9MYCE (Pleckstrin (PH) domain-containing protein n=1 Tax=Tieghemostelium lacteum TaxID=361077 RepID=A0A151Z724_9MYCE)

HSP 1 Score: 87.4 bits (215), Expect = 1.340e-17
Identity = 49/122 (40.16%), Postives = 73/122 (59.84%), Query Frame = 1

Query:    1 MEKGVVKLQALVRGHLARLRYAEISRHVAYRDKVAKEILATERSYVESLTTCLIVYMEPLKKTQRDSKPLLSPAQFKSIFSELPVLLNFNAELLGELEPLVEKWNAFQCIGPLFLKLVAFMK 366
            M   +  +Q+ VRG L RLRY E+ RH+A R+K A EI+ TER+YV++L   +  ++ PL+ T++D   LLS A+  S+FS   ++   + ELL  LE    KW+  Q +   FL L  F+K
Sbjct: 1120 MHNKITLIQSHVRGWLQRLRYREMMRHIALRNKCANEIMETERNYVQNLGILIDQFLVPLQTTKKD---LLSSAEITSVFSNCSLIHGVHKELLDSLEKKYAKWSNEQSLAVCFLDLTPFLK 1238          

BLAST of mRNA_M-pyrifera_M_contig112020.2514.1 vs. uniprot
Match: A0A7S4KYE4_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Paramoeba aestuarina TaxID=180227 RepID=A0A7S4KYE4_9EUKA)

HSP 1 Score: 85.5 bits (210), Expect = 1.530e-17
Identity = 35/96 (36.46%), Postives = 63/96 (65.62%), Query Frame = 1

Query:   79 HVAYRDKVAKEILATERSYVESLTTCLIVYMEPLKKTQRDSKPLLSPAQFKSIFSELPVLLNFNAELLGELEPLVEKWNAFQCIGPLFLKLVAFMK 366
            H   + K+ KE+L+TE +YV SL TC++V+++PL    +D +P++S  + K IF+ +  +  F+  LLG+++  +E+WN F CI  +FL  + ++K
Sbjct:   19 HETEKMKILKELLSTEETYVRSLDTCIVVWLDPLDARLKDKRPIISQDEIKVIFASIKTIFMFHTVLLGDIKSRLEQWNCFSCIADIFLGFLHYLK 114          

BLAST of mRNA_M-pyrifera_M_contig112020.2514.1 vs. uniprot
Match: L8HB33_ACACA (Calponin domain containing protein n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8HB33_ACACA)

HSP 1 Score: 85.9 bits (211), Expect = 4.450e-17
Identity = 49/121 (40.50%), Postives = 74/121 (61.16%), Query Frame = 1

Query:    4 EKGVVKLQALVRGHLARLRYAEISRHVAYRDKVAKEILATERSYVESLTTCLIVYMEPLKKTQRDSKPLLSPAQFKSIFSELPVLLNFNAELLGELEPLVEKWNAFQCIGPLFLKLVAFMK 366
            E  VV+LQA +RG +AR R+ E    +  R + A+E+L +E  YV+SL   + V++ PL+K        LSP Q K++FSE+ V++N+ A LL  +E  +  W   QC+G +FLKL  F+K
Sbjct:  525 EHLVVRLQAQLRGWVARKRFREQVYMLGQRTETARELLRSENIYVDSLRVIVEVFLTPLRKPGS----ALSPWQVKAVFSEVEVIMNYTAILLDRVEKKLAAWTHNQCLGEIFLKLTDFLK 641