mRNA_M-pyrifera_M_contig110685.2249.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig110685.2249.1
Unique NamemRNA_M-pyrifera_M_contig110685.2249.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig110685.2249.1 vs. uniprot
Match: A0A3D3BPL3_9ACTN (Transport permease protein n=1 Tax=Actinomycetia bacterium TaxID=1883427 RepID=A0A3D3BPL3_9ACTN)

HSP 1 Score: 195 bits (495), Expect = 2.860e-59
Identity = 93/171 (54.39%), Postives = 122/171 (71.35%), Query Frame = 1 
Query:    4 KTIYGNLYLIRSIYFPRAIVPLTSALENLYMFVPALAVMIGMAVITGEAPTWRWAVLPLLLLMLALMTIGIVFMSARAGYEVPDLYSLLPHLLRLLFYGSGVLFDPAAFTKNETVLFVFNLNPFFEMCALMRWSITGRAVDGWIWGAAIAWTLFFLVAGFVYFWRAEIKYG 516
            + +  NL LIRS+YFPRA +P++S L  +Y F P++ VM  + V +G  PTWRW +LPL+L+M+  MT G VF+ AR G  VPDL+SLLPHL+RLLFY SGVLF PA+F+ N TVL++F LNPFFEM  ++RW   GR++DG +WG    W +  +V G  YFWR EI YG
Sbjct:  110 RCLQANLTLIRSMYFPRAAIPISSTLSGVYTFAPSILVMALVVVGSGHWPTWRWLLLPLVLMMMVTMTQGAVFVVARVGKAVPDLHSLLPHLIRLLFYCSGVLFAPASFSDNATVLWLFKLNPFFEMLEMLRWVFMGRSIDGELWGFGAGWAIVSVVGGAWYFWRGEITYG 280
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BLAST of mRNA_M-pyrifera_M_contig110685.2249.1 vs. uniprot
Match: A0A800IEA7_9ACTN (Transport permease protein n=1 Tax=Acidimicrobiia bacterium TaxID=2080302 RepID=A0A800IEA7_9ACTN)

HSP 1 Score: 138 bits (347), Expect = 4.470e-37
Identity = 71/170 (41.76%), Postives = 100/170 (58.82%), Query Frame = 1 
Query:    7 TIYGNLYLIRSIYFPRAIVPLTSALENLYMFVPALAVMIGMAVITGEAPTWRWAVLPLLLLMLALMTIGIVFMSARAGYEVPDLYSLLPHLLRLLFYGSGVLFDPAAFTKNETVLFVFNLNPFFEMCALMRWSITGRAVDGWIWGAAIAWTLFFLVAGFVYFWRAEIKYG 516
            +I  N  LIRSI FPR+++P+++       F P+L V++G  +ITGE PTWRW  LP++LL  AL  +G  F+ AR G+ V D+  +LPHL RLLFY SGV+F    F  N T+  +F LNP +++  + RWS+ G           I W     +AG +YF   E +YG
Sbjct:  107 SIKRNESLIRSIQFPRSLLPVSAVTGQTIAFAPSLVVLLGFVLITGELPTWRWFALPVILLGQALFGVGAAFIIARVGFAVTDIQQILPHLFRLLFYMSGVIFSVEVFITNTTMQNLFALNPMYDIITIARWSLMGLPAPTACVVGLIVWATLLPIAGLLYFRAGEHRYG 276
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BLAST of mRNA_M-pyrifera_M_contig110685.2249.1 vs. uniprot
Match: A0A2E7KXS3_9BACT (Transport permease protein n=1 Tax=Verrucomicrobiaceae bacterium TaxID=2026800 RepID=A0A2E7KXS3_9BACT)

HSP 1 Score: 130 bits (328), Expect = 3.050e-34
Identity = 66/172 (38.37%), Postives = 102/172 (59.30%), Query Frame = 1 
Query:    1 TKTIYGNLYLIRSIYFPRAIVPLTSALENLYMFVPALAVMIGMAVITGEAPTWRWAVLPLLLLMLALMTIGIVFMSARAGYEVPDLYSLLPHLLRLLFYGSGVLFDPAAFTKNETVLFVFNLNPFFEMCALMRWSITGRAVDGWIWGAAIAWTLFFLVAGFVYFWRAEIKYG 516
            T +I  N  LIRSI FPRA++P+ + +     F PA+ V     +  G +P++RW ++  ++ +  L  +G  F+ AR GY  PDL  LLPH  RLL YGSG++F   +F  N+ VL ++ +NP +++C L RW++ G  + G    A I W++   V G +YF R E++YG
Sbjct:  105 TVSIRRNQSLIRSIQFPRALLPIGAIVGQTIAFFPAMVVTFVFLISLGVSPSFRWFLILPIIFLQQLFGLGAGFVFARLGYSFPDLQQLLPHFFRLLLYGSGIIFSLESFVTNKLVLDLYVINPLYDICTLARWALIGYPISGLCVLALIGWSIILPVFGLLYFRRQELRYG 276
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BLAST of mRNA_M-pyrifera_M_contig110685.2249.1 vs. uniprot
Match: A0A800ID69_9ACTN (Transport permease protein n=1 Tax=Acidimicrobiia bacterium TaxID=2080302 RepID=A0A800ID69_9ACTN)

HSP 1 Score: 129 bits (323), Expect = 1.700e-33
Identity = 67/170 (39.41%), Postives = 101/170 (59.41%), Query Frame = 1 
Query:    7 TIYGNLYLIRSIYFPRAIVPLTSALENLYMFVPALAVMIGMAVITGEAPTWRWAVLPLLLLMLALMTIGIVFMSARAGYEVPDLYSLLPHLLRLLFYGSGVLFDPAAFTKNETVLFVFNLNPFFEMCALMRWSITGRAVDGWIWGAAIAWTLFFLVAGFVYFWRAEIKYG 516
            +I  N  LIRSI FPR+++P+++       F+P+L V++   +ITGE PT RW +LP++LL  AL  +G  F+ AR G+ V D+  +LPHL RLLFY SGV+F    F  N T+  +F LNP +++    RW++ G  +        + W L   +AG ++F   E +YG
Sbjct:  107 SIRRNESLIRSIQFPRSLLPVSAVTGQTIAFIPSLVVLLSFVLITGERPTLRWLLLPIILLGQALFGVGASFIIARIGFAVTDIQQILPHLFRLLFYMSGVIFSVEVFITNTTMQNLFALNPMYDIITAARWALMGLPLPVACVVGLVLWALLLPIAGLLFFRAREHRYG 276
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BLAST of mRNA_M-pyrifera_M_contig110685.2249.1 vs. uniprot
Match: A0A2E8NB55_9ACTN (Transport permease protein n=2 Tax=Acidimicrobiaceae bacterium TaxID=2024894 RepID=A0A2E8NB55_9ACTN)

HSP 1 Score: 129 bits (323), Expect = 1.950e-33
Identity = 69/172 (40.12%), Postives = 97/172 (56.40%), Query Frame = 1 
Query:    4 KTIYGNLYLIRSIYFPRAIVPLTSALENLYMFVPALAVMIGMAVITGEAPTWRWAVLPLLLLMLALMTIGIVFMSARAGYEVPDLYSLLPHLLRLLFYGSGVLFDPAAFTKNETVLFVFNLNPFFEMCALMRWSITGRAVDGWIWGAAIAWTLFFLVAGFVYFWRAEIKYGG 519
            +++  N  L+R++ FPRA +P+ SA+  L  F P L VM+ + +  G AP  RW  LP LL  L  + +G   +SARA +   DL +LLP L RLLFY SGVL+    F  +E +  +F +NP + +    RW++ G  V G  W   +AW   FL+ GFV F   E  YGG
Sbjct:  110 RSVVANAGLVRALRFPRAALPVGSAIGQLLSFAPVLVVMLVVTLAHGHAPDLRWLALPGLLAALTALGLGAGLLSARATHTYRDLENLLPFLFRLLFYLSGVLYSVDHFVADEALRALFAVNPLYCLVTTWRWALIGTEVSGLAWAGTVAWPTLFLITGFVAFRAREATYGG 281
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BLAST of mRNA_M-pyrifera_M_contig110685.2249.1 vs. uniprot
Match: A0A6N8UQC1_9ACTN (Transport permease protein n=4 Tax=Acidimicrobiia TaxID=84992 RepID=A0A6N8UQC1_9ACTN)

HSP 1 Score: 127 bits (319), Expect = 6.380e-33
Identity = 66/166 (39.76%), Postives = 91/166 (54.82%), Query Frame = 1 
Query:   19 NLYLIRSIYFPRAIVPLTSALENLYMFVPALAVMIGMAVITGEAPTWRWAVLPLLLLMLALMTIGIVFMSARAGYEVPDLYSLLPHLLRLLFYGSGVLFDPAAFTKNETVLFVFNLNPFFEMCALMRWSITGRAVDGWIWGAAIAWTLFFLVAGFVYFWRAEIKYG 516
            NL LIRS+ FPR ++P  +       FVPAL + +   + TGE P+ RW  L  +L    +   G   + AR G  VPDL  LLPH+ RLLFY SGV+F   AF  +E     F LNP +++    RW + G  VD W+    +AW +  L+ GF  FWR++ + G
Sbjct:  109 NLGLIRSVQFPRILLPAATVNGQTAAFVPALVLALAAVIATGERPSLRWLALIGVLAAQFVFNFGAALLVARIGAAVPDLRQLLPHVFRLLFYASGVIFSVDAFVTSEFWRRAFALNPIYDVITCARWCLLGEPVDSWVVAGMLAWGVVLLLVGFAVFWRSDQRLG 274
[Close]
BLAST of mRNA_M-pyrifera_M_contig110685.2249.1 vs. uniprot
Match: A0A6G2JWP9_9ACTN (Transport permease protein n=3 Tax=Acidimicrobiales bacterium TaxID=2201156 RepID=A0A6G2JWP9_9ACTN)

HSP 1 Score: 127 bits (320), Expect = 8.870e-33
Identity = 70/172 (40.70%), Postives = 93/172 (54.07%), Query Frame = 1 
Query:    1 TKTIYGNLYLIRSIYFPRAIVPLTSALENLYMFVPALAVMIGMAVITGEAPTWRWAVLPLLLLMLALMTIGIVFMSARAGYEVPDLYSLLPHLLRLLFYGSGVLFDPAAFTKNETVLFVFNLNPFFEMCALMRWSITGRAVDGWIWGAAIAWTLFFLVAGFVYFWRAEIKYG 516
            + +I  N+ LIRSI FPR ++P  S       FVPALA+ I   + TGE PT RW VLP +L        G   + AR G  +PDL  +LPHL RLLFYGSGV+F   AF ++      F LNP +++    RW + G  VD W+    + W +     GF  F R+E + G
Sbjct:  133 STSITQNMGLIRSIQFPRILLPAASVNGQTAAFVPALALAILAVLATGELPTLRWLVLPAVLAAQLAFNFGTALLVARIGTTLPDLRQILPHLFRLLFYGSGVIFSVDAFVQSTAWRRAFALNPLYDVITCARWCLLGEPVDLWVVAGLVMWCVLMPAIGFAVFHRSEQRLG 304
[Close]
BLAST of mRNA_M-pyrifera_M_contig110685.2249.1 vs. uniprot
Match: A0A7K2BTW9_9ACTN (Transport permease protein n=2 Tax=Acidimicrobiaceae bacterium TaxID=2024894 RepID=A0A7K2BTW9_9ACTN)

HSP 1 Score: 126 bits (316), Expect = 1.780e-32
Identity = 68/166 (40.96%), Postives = 89/166 (53.61%), Query Frame = 1 
Query:   19 NLYLIRSIYFPRAIVPLTSALENLYMFVPALAVMIGMAVITGEAPTWRWAVLPLLLLMLALMTIGIVFMSARAGYEVPDLYSLLPHLLRLLFYGSGVLFDPAAFTKNETVLFVFNLNPFFEMCALMRWSITGRAVDGWIWGAAIAWTLFFLVAGFVYFWRAEIKYG 516
            NL LIRS+ FPR ++P  +       FVPAL + +   V TGE P+ RW  LP +L    L   G   + AR G  + DL  LLPHL RLLFY SGV+F   AF  +      F LNP +++    RW + G   DGW+    +AW +   V GFV F R+E + G
Sbjct:  109 NLGLIRSVQFPRILLPAATLNGQTAAFVPALVLAMVAVVATGERPSMRWLALPAVLAAQFLFNFGAALLVARIGASLSDLQQLLPHLFRLLFYASGVIFSVEAFVTSPAWRRAFALNPIYDVITCARWCLVGELADGWVVAGLLAWCIALPVIGFVVFHRSEQRLG 274
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BLAST of mRNA_M-pyrifera_M_contig110685.2249.1 vs. uniprot
Match: A0A6B1B559_9ACTN (Transport permease protein n=1 Tax=Acidimicrobiales bacterium TaxID=2201156 RepID=A0A6B1B559_9ACTN)

HSP 1 Score: 125 bits (314), Expect = 5.180e-32
Identity = 69/170 (40.59%), Postives = 93/170 (54.71%), Query Frame = 1 
Query:    7 TIYGNLYLIRSIYFPRAIVPLTSALENLYMFVPALAVMIGMAVITGEAPTWRWAVLPLLLLMLALMTIGIVFMSARAGYEVPDLYSLLPHLLRLLFYGSGVLFDPAAFTKNETVLFVFNLNPFFEMCALMRWSITGRAVDGWIWGAAIAWTLFFLVAGFVYFWRAEIKYG 516
            +I  NL LIRSI FPR ++P  S       FVPAL + +   + TGE PT RW VLP +L+       G+  + AR G  + DL  +LPHL RLLFYGSGV+F   AF ++      F LNP +++    RW + G  VD W+    + W +     GFV F R+E + G
Sbjct:  122 SITQNLGLIRSIQFPRILLPAASVNGQTAAFVPALVLALLAVLATGELPTLRWLVLPAVLVAQFAFNFGVALLVARIGTTMRDLRQILPHLFRLLFYGSGVIFSVDAFVQSTAWRRAFALNPVYDVITCARWCLLGEPVDLWVVAGLVLWCIVMPALGFVVFHRSEQRLG 291
[Close]
BLAST of mRNA_M-pyrifera_M_contig110685.2249.1 vs. uniprot
Match: A0A2E5NS57_9ACTN (Transport permease protein n=1 Tax=Acidimicrobiaceae bacterium TaxID=2024894 RepID=A0A2E5NS57_9ACTN)

HSP 1 Score: 124 bits (312), Expect = 9.390e-32
Identity = 66/171 (38.60%), Postives = 97/171 (56.73%), Query Frame = 1 
Query:    4 KTIYGNLYLIRSIYFPRAIVPLTSALENLYMFVPALAVMIGMAVITGEAPTWRWAVLPLLLLMLALMTIGIVFMSARAGYEVPDLYSLLPHLLRLLFYGSGVLFDPAAFTKNETVLFVFNLNPFFEMCALMRWSITGRAVDGWIWGAAIAWTLFFLVAGFVYFWRAEIKYG 516
            +++  N  L+R++ FPRA +P+ SA+  L  F P L VM+ + +  G AP  RW  LP +LL L    +G   ++ARA +   DL +LLP L RLLFY SGVL+    F  +E +  +F L+P + +  + RW++ G  V G +W   + W +  LV GFV F   E  YG
Sbjct:  115 RSVVSNAGLVRALRFPRAALPVGSAIGQLLSFAPVLVVMLAVTLAHGHAPNLRWISLPAILLALTAFGLGAGLLAARATHTYRDLENLLPFLFRLLFYLSGVLYSVDHFIDDEALRALFALDPLYCLITIWRWALIGTEVSGPVWAGTVIWPIITLVLGFVAFRSREATYG 285
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig110685.2249.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A3D3BPL3_9ACTN2.860e-5954.39Transport permease protein n=1 Tax=Actinomycetia b... [more]
A0A800IEA7_9ACTN4.470e-3741.76Transport permease protein n=1 Tax=Acidimicrobiia ... [more]
A0A2E7KXS3_9BACT3.050e-3438.37Transport permease protein n=1 Tax=Verrucomicrobia... [more]
A0A800ID69_9ACTN1.700e-3339.41Transport permease protein n=1 Tax=Acidimicrobiia ... [more]
A0A2E8NB55_9ACTN1.950e-3340.12Transport permease protein n=2 Tax=Acidimicrobiace... [more]
A0A6N8UQC1_9ACTN6.380e-3339.76Transport permease protein n=4 Tax=Acidimicrobiia ... [more]
A0A6G2JWP9_9ACTN8.870e-3340.70Transport permease protein n=3 Tax=Acidimicrobiale... [more]
A0A7K2BTW9_9ACTN1.780e-3240.96Transport permease protein n=2 Tax=Acidimicrobiace... [more]
A0A6B1B559_9ACTN5.180e-3240.59Transport permease protein n=1 Tax=Acidimicrobiale... [more]
A0A2E5NS57_9ACTN9.390e-3238.60Transport permease protein n=1 Tax=Acidimicrobiace... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig110685contigM-pyrifera_M_contig110685:68..595 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score119.4
Seed ortholog evalue1.7e-24
Seed eggNOG ortholog1033730.CAHG01000002_gene2880
Preferred nametagG
KEGG koko:K09690,ko:K09692
KEGG TC3.A.1.103,3.A.1.104
KEGG Pathwayko02010,map02010
KEGG ModuleM00250,M00251
Hectar predicted targeting categorysignal anchor
EggNOG free text desc.ABC-2 type transporter
EggNOG OGs2GP5K@201174,4DQPR@85009,COG1682@1,COG1682@2
COG Functional cat.GM
Best tax levelPropionibacteriales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko02000
Exons1
Model size528
Cds size528
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815619.030531-CDS-M-pyrifera_M_contig110685:67..5951622815619.030531-CDS-M-pyrifera_M_contig110685:67..595Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig110685 68..595 -
1692277490.3720798-CDS-M-pyrifera_M_contig110685:67..5951692277490.3720798-CDS-M-pyrifera_M_contig110685:67..595Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig110685 68..595 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig110685.2249.1prot_M-pyrifera_M_contig110685.2249.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig110685 68..595 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig110685.2249.1

>prot_M-pyrifera_M_contig110685.2249.1 ID=prot_M-pyrifera_M_contig110685.2249.1|Name=mRNA_M-pyrifera_M_contig110685.2249.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=176bp
TKTIYGNLYLIRSIYFPRAIVPLTSALENLYMFVPALAVMIGMAVITGEA
PTWRWAVLPLLLLMLALMTIGIVFMSARAGYEVPDLYSLLPHLLRLLFYG
SGVLFDPAAFTKNETVLFVFNLNPFFEMCALMRWSITGRAVDGWIWGAAI
AWTLFFLVAGFVYFWRAEIKYGGVQ*
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mRNA from alignment at M-pyrifera_M_contig110685:68..595-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig110685.2249.1 ID=mRNA_M-pyrifera_M_contig110685.2249.1|Name=mRNA_M-pyrifera_M_contig110685.2249.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=528bp|location=Sequence derived from alignment at M-pyrifera_M_contig110685:68..595- (Macrocystis pyrifera P11B4 male)
ACGAAGACGATCTACGGCAACCTCTATCTGATCCGTTCGATCTACTTCCC
TCGAGCGATCGTGCCGCTCACCTCCGCCTTGGAAAACCTCTACATGTTCG
TCCCCGCGCTCGCGGTGATGATCGGCATGGCGGTCATCACCGGCGAAGCA
CCGACATGGCGCTGGGCCGTCCTGCCGTTGCTGTTGCTGATGCTGGCACT
CATGACCATCGGCATCGTGTTCATGTCGGCCCGAGCCGGGTACGAGGTAC
CCGACCTCTACAGCCTTCTTCCCCACCTGCTCCGTCTGCTGTTCTACGGG
TCGGGCGTGCTCTTCGATCCAGCCGCGTTCACCAAGAACGAGACGGTGCT
CTTCGTCTTCAACCTCAATCCGTTCTTCGAGATGTGCGCGCTCATGCGAT
GGTCGATCACCGGTCGAGCCGTCGACGGTTGGATATGGGGTGCCGCAATT
GCCTGGACACTCTTCTTCCTCGTCGCCGGCTTCGTCTACTTCTGGCGGGC
CGAGATCAAGTACGGCGGCGTCCAGTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig110685:68..595-

>mRNA_M-pyrifera_M_contig110685.2249.1 ID=mRNA_M-pyrifera_M_contig110685.2249.1|Name=mRNA_M-pyrifera_M_contig110685.2249.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1056bp|location=Sequence derived from alignment at M-pyrifera_M_contig110685:68..595- (Macrocystis pyrifera P11B4 male)
ACGAAGACGATCTACGGCAACCTCTATCTGATCCGTTCGATCTACTTCCC
TCGAGCGATCGTGCCGCTCACCTCCGCCTTGGAAAACCTCTACATGTTCG
TCCCCGCGCTCGCGGTGATGATCGGCATGGCGGTCATCACCGGCGAAGCA
CCGACATGGCGCTGGGCCGTCCTGCCGTTGCTGTTGCTGATGCTGGCACT
CATGACCATCGGCATCGTGTTCATGTCGGCCCGAGCCGGGTACGAGGTAC
CCGACCTCTACAGCCTTCTTCCCCACCTGCTCCGTCTGCTGTTCTACGGG
TCGGGCGTGCTCTTCGATCCAGCCGCGTTCACCAAGAACGAGACGGTGCT
CTTCGTCTTCAACCTCAATCCGTTCTTCGAGATGTGCGCGCTCATGCGAT
GGTCGATCACCGGTCGAGCCGTCGACGGTTGGATATGGGGTGCCGCAATT
GCCTGGACACTCTTCTTCCTCGTCGCCGGCTTCGTCTACTTCTGGCGGGC
CGAGATCAAGTACGGCGGCGTCCAGTGAACGAAGACGATCTACGGCAACC
TCTATCTGATCCGTTCGATCTACTTCCCTCGAGCGATCGTGCCGCTCACC
TCCGCCTTGGAAAACCTCTACATGTTCGTCCCCGCGCTCGCGGTGATGAT
CGGCATGGCGGTCATCACCGGCGAAGCACCGACATGGCGCTGGGCCGTCC
TGCCGTTGCTGTTGCTGATGCTGGCACTCATGACCATCGGCATCGTGTTC
ATGTCGGCCCGAGCCGGGTACGAGGTACCCGACCTCTACAGCCTTCTTCC
CCACCTGCTCCGTCTGCTGTTCTACGGGTCGGGCGTGCTCTTCGATCCAG
CCGCGTTCACCAAGAACGAGACGGTGCTCTTCGTCTTCAACCTCAATCCG
TTCTTCGAGATGTGCGCGCTCATGCGATGGTCGATCACCGGTCGAGCCGT
CGACGGTTGGATATGGGGTGCCGCAATTGCCTGGACACTCTTCTTCCTCG
TCGCCGGCTTCGTCTACTTCTGGCGGGCCGAGATCAAGTACGGCGGCGTC
CAGTGA
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