mRNA_M-pyrifera_M_contig106787.1455.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig106787.1455.1
Unique NamemRNA_M-pyrifera_M_contig106787.1455.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig106787.1455.1 vs. uniprot
Match: A0A1I3V614_9GAMM (ABC transporter n=2 Tax=Methylophaga sulfidovorans TaxID=45496 RepID=A0A1I3V614_9GAMM)

HSP 1 Score: 209 bits (531), Expect = 8.770e-66
Identity = 97/172 (56.40%), Postives = 132/172 (76.74%), Query Frame = 1 
Query:    1 GAIRVDGTDIAQIDPADLRRQIGFVQQDPRLFYGTARENITMKAPWVTDEEVLRVAKISGADSFIGKHPAGYDMPIGEGGQGISGGQCQAICIARSLLLSPPIMLFDEPTSAMDNSSEQWFMENFTKAFEGKTLLLVTHKMSILGMVDRLIVLQHGKIVADGSKERVLSALK 516
            G+I +DGTDI QIDP DLR+ +G+  QD  LFYG+ RENITM AP+  D EVL+ A I+G   F+ +HP+G+DMP+GE G+G+SGGQ Q++ +AR+LLL  P+++ DEPT+AMDNS+E+ F     +  E KTLLLVTHK S+L +VDR+IV+  G IVADG +E++L+ALK
Sbjct:   10 GSILLDGTDIRQIDPVDLRKNVGYAPQDVVLFYGSIRENITMVAPFAEDHEVLKAADIAGVTEFVNRHPSGFDMPVGERGEGLSGGQRQSVAVARALLLDSPVIMLDEPTNAMDNSTEERFKAKLAENVENKTLLLVTHKASLLSLVDRVIVMDQGHIVADGPREQILTALK 181
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BLAST of mRNA_M-pyrifera_M_contig106787.1455.1 vs. uniprot
Match: A0A2E3MIY7_9GAMM (Type I secretion system permease/ATPase n=1 Tax=Legionellales bacterium TaxID=2026754 RepID=A0A2E3MIY7_9GAMM)

HSP 1 Score: 222 bits (565), Expect = 3.370e-65
Identity = 103/185 (55.68%), Postives = 147/185 (79.46%), Query Frame = 1 
Query:    1 GAIRVDGTDIAQIDPADLRRQIGFVQQDPRLFYGTARENITMKAPWVTDEEVLRVAKISGADSFIGKHPAGYDMPIGEGGQGISGGQCQAICIARSLLLSPPIMLFDEPTSAMDNSSEQWFMENFTKAFEGKTLLLVTHKMSILGMVDRLIVLQHGKIVADGSKERVLSALKQLQQKKSPSEVGD 555
            GAI++DGTDI+QIDPADLRR +G+VQQ+P++F+G+ R+NI ++APWV D E+LR A+ +GA +FIG  P G+D  IGE G G+SGGQ Q I +AR+LL+SP IMLFDEP+S+MDNS+E  F++N  +  + KT +LVTHK+ +L +V R++VLQHGKI+ADG K++VL  L+Q++ ++S  E  +
Sbjct:  543 GAIKIDGTDISQIDPADLRRNLGYVQQEPKVFFGSVRDNIAVRAPWVDDTEILRAAQAAGAYNFIGAMPNGFDALIGENGVGLSGGQIQTITVARALLMSPSIMLFDEPSSSMDNSTEAAFVKNMQETLKDKTAILVTHKIPLLQIVSRILVLQHGKIIADGPKDKVLEFLQQVRNERSNQEQAE 727
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BLAST of mRNA_M-pyrifera_M_contig106787.1455.1 vs. uniprot
Match: UPI00112DF6D0 (type I secretion system permease/ATPase n=1 Tax=Methylomonas koyamae TaxID=702114 RepID=UPI00112DF6D0)

HSP 1 Score: 219 bits (557), Expect = 3.890e-64
Identity = 103/177 (58.19%), Postives = 140/177 (79.10%), Query Frame = 1 
Query:    1 GAIRVDGTDIAQIDPADLRRQIGFVQQDPRLFYGTARENITMKAPWVTDEEVLRVAKISGADSFIGKHPAGYDMPIGEGGQGISGGQCQAICIARSLLLSPPIMLFDEPTSAMDNSSEQWFMENFTKAFEGKTLLLVTHKMSILGMVDRLIVLQHGKIVADGSKERVLSALKQLQQK 531
            GA+ +DGTDI Q+DPADLRRQIG+V QD  L YG+ ++NIT+ + +  D++VLR A+I+G D+F+ KHPAG+D+ +GEGG  +SGGQ Q+I +AR+L+LSPPI + DEPT+AMDNSSE+ F + F    +G+TL+LVTH+MS+L +VDRLIV+  G IVADG K+ VL AL+Q Q K
Sbjct:  537 GAVLIDGTDIRQLDPADLRRQIGYVPQDISLLYGSVKDNITLGSGFADDQQVLRAARIAGVDAFVNKHPAGFDLQVGEGGANLSGGQRQSIALARALVLSPPIFVLDEPTNAMDNSSEEGFKQRFAAELQGQTLILVTHRMSLLSLVDRLIVMDGGHIVADGPKDHVLEALRQGQIK 713
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BLAST of mRNA_M-pyrifera_M_contig106787.1455.1 vs. uniprot
Match: A0A3D1CKF9_9GAMM (Type I secretion system permease/ATPase (Fragment) n=1 Tax=Methylophaga sp. TaxID=2024840 RepID=A0A3D1CKF9_9GAMM)

HSP 1 Score: 201 bits (512), Expect = 2.250e-62
Identity = 91/172 (52.91%), Postives = 132/172 (76.74%), Query Frame = 1 
Query:    1 GAIRVDGTDIAQIDPADLRRQIGFVQQDPRLFYGTARENITMKAPWVTDEEVLRVAKISGADSFIGKHPAGYDMPIGEGGQGISGGQCQAICIARSLLLSPPIMLFDEPTSAMDNSSEQWFMENFTKAFEGKTLLLVTHKMSILGMVDRLIVLQHGKIVADGSKERVLSALK 516
            GA+ +D TD+ QIDP DLRR +G+V QD  LFYG+ R+NI + AP+  D  VLR A+I+G   FI +HP+G+DMP+GE G+G+SGGQ Q+I +AR+LLL PP+++ DEPT++MDNSSE+ F     +  + K+L+LVTH+ S+L +VDR++V+  G +VADG +E++L+ALK
Sbjct:   48 GAVLLDETDLRQIDPIDLRRSVGYVPQDVMLFYGSVRDNIALGAPFADDAAVLRAAEIAGVTEFINRHPSGFDMPVGERGEGLSGGQRQSIAVARALLLDPPVLVLDEPTNSMDNSSEERFKTKLGEIIKDKSLILVTHRASLLSLVDRVLVMDQGNLVADGPREQILAALK 219
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BLAST of mRNA_M-pyrifera_M_contig106787.1455.1 vs. uniprot
Match: UPI00034CD0C3 (ATP-binding cassette domain-containing protein n=1 Tax=Methylophaga lonarensis TaxID=999151 RepID=UPI00034CD0C3)

HSP 1 Score: 206 bits (525), Expect = 2.560e-62
Identity = 90/172 (52.33%), Postives = 134/172 (77.91%), Query Frame = 1 
Query:    1 GAIRVDGTDIAQIDPADLRRQIGFVQQDPRLFYGTARENITMKAPWVTDEEVLRVAKISGADSFIGKHPAGYDMPIGEGGQGISGGQCQAICIARSLLLSPPIMLFDEPTSAMDNSSEQWFMENFTKAFEGKTLLLVTHKMSILGMVDRLIVLQHGKIVADGSKERVLSALK 516
            GA+ +DGTD+ QIDP DLRR +G+  QD  LFYG+ R+NI + AP+  D+ +L+ A+++G   F+ +HP+G+DM +GE G+G+SGGQ Q+I +AR+LL+ PP+++ DEPT+AMDNS+E+ F    T+  E KTLLLVTH+ S+L +VDR+IV+  GK+VADG +E++++ALK
Sbjct:  209 GAVLLDGTDLRQIDPVDLRRSVGYAPQDVVLFYGSVRDNIAIGAPYADDQAILKAAELAGVTEFVNRHPSGFDMQVGERGEGLSGGQRQSIAVARALLMDPPMLVLDEPTNAMDNSTEERFKSKLTQVLENKTLLLVTHRASLLSLVDRIIVMDQGKLVADGPREQIMTALK 380
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BLAST of mRNA_M-pyrifera_M_contig106787.1455.1 vs. uniprot
Match: A0A1Y5DQK9_9GAMM (ABC transporter n=3 Tax=unclassified Methylophaga TaxID=2629249 RepID=A0A1Y5DQK9_9GAMM)

HSP 1 Score: 210 bits (534), Expect = 6.890e-61
Identity = 95/172 (55.23%), Postives = 133/172 (77.33%), Query Frame = 1 
Query:    1 GAIRVDGTDIAQIDPADLRRQIGFVQQDPRLFYGTARENITMKAPWVTDEEVLRVAKISGADSFIGKHPAGYDMPIGEGGQGISGGQCQAICIARSLLLSPPIMLFDEPTSAMDNSSEQWFMENFTKAFEGKTLLLVTHKMSILGMVDRLIVLQHGKIVADGSKERVLSALK 516
            GAI +DGTDI Q+DP DLRR +G+  QD  LF+G+ R+NI + AP+  D  VL+ A+I+G   F+ +HP+G+DMP+GE G+G+SGGQ Q++ +AR+LLL PP+++ DEPT+AMDNS+E+ F        +GKTLL+VTHK S+L +VDR+IVL  G++VADG +E+VLSALK
Sbjct:  523 GAILLDGTDIRQVDPVDLRRNVGYAPQDVVLFFGSIRDNIALGAPFADDNAVLKAAEITGVSEFVNRHPSGFDMPVGERGEGLSGGQRQSVAVARALLLDPPVLVLDEPTNAMDNSTEESFKTKLAGMLDGKTLLIVTHKASLLSLVDRIIVLDQGRLVADGPREQVLSALK 694
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BLAST of mRNA_M-pyrifera_M_contig106787.1455.1 vs. uniprot
Match: C0N3R0_9GAMM (ABC transporter, ATP-binding protein n=1 Tax=Methylophaga thiooxydans DMS010 TaxID=637616 RepID=C0N3R0_9GAMM)

HSP 1 Score: 204 bits (520), Expect = 9.490e-61
Identity = 92/172 (53.49%), Postives = 134/172 (77.91%), Query Frame = 1 
Query:    1 GAIRVDGTDIAQIDPADLRRQIGFVQQDPRLFYGTARENITMKAPWVTDEEVLRVAKISGADSFIGKHPAGYDMPIGEGGQGISGGQCQAICIARSLLLSPPIMLFDEPTSAMDNSSEQWFMENFTKAFEGKTLLLVTHKMSILGMVDRLIVLQHGKIVADGSKERVLSALK 516
            GAI +DGTDI Q+DP DLRR +G+  QD  LF+G+ R+NITM AP+  D+ VL+ A ++G   F+ +HP+G+DMP+GE G+G+SGGQ Q++ +AR+LLL P +++ DEPT+AMDNS+E+ F    +++ + KTLLLVTHK S+L +VDR+IV+  G +VADG +E++L+ALK
Sbjct:  286 GAILLDGTDIRQVDPVDLRRSVGYAPQDVVLFFGSIRDNITMVAPFADDKMVLKAADVAGVSEFVNRHPSGFDMPVGERGEGLSGGQRQSVAVARALLLDPSVVVLDEPTNAMDNSTEERFKAKLSESLKEKTLLLVTHKASLLSLVDRIIVMDQGHLVADGPREQILAALK 457
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BLAST of mRNA_M-pyrifera_M_contig106787.1455.1 vs. uniprot
Match: A0A661DGQ9_9GAMM (Type I secretion system permease/ATPase n=2 Tax=Gammaproteobacteria TaxID=1236 RepID=A0A661DGQ9_9GAMM)

HSP 1 Score: 209 bits (532), Expect = 1.720e-60
Identity = 97/172 (56.40%), Postives = 132/172 (76.74%), Query Frame = 1 
Query:    1 GAIRVDGTDIAQIDPADLRRQIGFVQQDPRLFYGTARENITMKAPWVTDEEVLRVAKISGADSFIGKHPAGYDMPIGEGGQGISGGQCQAICIARSLLLSPPIMLFDEPTSAMDNSSEQWFMENFTKAFEGKTLLLVTHKMSILGMVDRLIVLQHGKIVADGSKERVLSALK 516
            GAI +DGTDI QIDP DLR+ IG+  QD  LF+G+ R+NI +  P+  D++VL  AKI+G   F+ +HP+G+DMP+GE G+G+SGGQ Q++ +AR+LLL P +++ DEPT+AMDNS+E+ F EN  +    KTLLLVTHK S+L +VDRLIVL  G++VADG +E+VL ALK
Sbjct:  541 GAILLDGTDIRQIDPVDLRKNIGYAPQDVVLFFGSIRDNIALGKPFAADDDVLHAAKIAGVTEFVNRHPSGFDMPVGERGEGLSGGQRQSVAVARALLLEPSVLVLDEPTNAMDNSTEEVFKENLVETLADKTLLLVTHKASLLSLVDRLIVLDQGQLVADGPREQVLEALK 712
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BLAST of mRNA_M-pyrifera_M_contig106787.1455.1 vs. uniprot
Match: A0A369CBV9_9GAMM (ATP-binding cassette subfamily C protein LapB n=1 Tax=Thioalbus denitrificans TaxID=547122 RepID=A0A369CBV9_9GAMM)

HSP 1 Score: 209 bits (532), Expect = 1.870e-60
Identity = 99/173 (57.23%), Postives = 133/173 (76.88%), Query Frame = 1 
Query:    1 GAIRVDGTDIAQIDPADLRRQIGFVQQDPRLFYGTARENITMKAPWVTDEEVLRVAKISGADSFIGKHPAGYDMPIGEGGQGISGGQCQAICIARSLLLSPPIMLFDEPTSAMDNSSEQWFMENFTKAFEGKTLLLVTHKMSILGMVDRLIVLQHGKIVADGSKERVLSALKQ 519
            GA+R+DG D+ QIDPADLRR  G+V QD  LFYG+ R+NI + AP+  D EVLRVA++SG   F+  +PAG+DM +GE G+ +SGGQ QAI IAR+LLL PPI+L DEPT++MDN++E+ F     +   GKT++LVTH+ S+L +VDR++V+  G+IVADG KE+VL ALKQ
Sbjct:  548 GAVRIDGIDLRQIDPADLRRNTGYVPQDTLLFYGSVRDNIRLGAPFADDREVLRVAELSGVTDFVNLNPAGFDMMVGERGETLSGGQRQAIAIARALLLEPPILLLDEPTNSMDNTTEEQFKTRMVQVLAGKTVVLVTHRASLLNLVDRIVVMDSGRIVADGPKEQVLEALKQ 720
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BLAST of mRNA_M-pyrifera_M_contig106787.1455.1 vs. uniprot
Match: A0A4S1E5J8_9BURK (ATP-binding cassette domain-containing protein (Fragment) n=1 Tax=Alcaligenaceae bacterium 429 TaxID=2562948 RepID=A0A4S1E5J8_9BURK)

HSP 1 Score: 196 bits (498), Expect = 2.250e-60
Identity = 95/173 (54.91%), Postives = 126/173 (72.83%), Query Frame = 1 
Query:    1 GAIRVDGTDIAQIDPADLRRQIGFVQQDPRLFYGTARENITMKAPWVTDEEVLRVAKISGADSFIGKHPAGYDMPIGEGGQGISGGQCQAICIARSLLLSPPIMLFDEPTSAMDNSSEQWFMENFTKAFEGKTLLLVTHKMSILGMVDRLIVLQHGKIVADGSKERVLSALKQ 519
            G + VDG DI QIDPADLRR +GFV QDP LFYGT + N+TM AP+ +DEE+L  A+ISG D F  +HP GYDM I E G+ +SGGQ Q+I +AR++L  P ++L DEP+S MDN SE             KT++LVTH+ ++L +VDRLIV+ +G+IVADG KE+V++ALK+
Sbjct:   37 GTVLVDGIDIRQIDPADLRRSMGFVSQDPVLFYGTLKHNLTMGAPYASDEEMLAAARISGVDQFASRHPDGYDMIISERGESLSGGQRQSIAVARAVLNRPSVLLLDEPSSHMDNQSEMALRSRLKNICVDKTVILVTHRTALLDLVDRLIVMDNGRIVADGPKEQVVTALKE 209
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig106787.1455.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A1I3V614_9GAMM8.770e-6656.40ABC transporter n=2 Tax=Methylophaga sulfidovorans... [more]
A0A2E3MIY7_9GAMM3.370e-6555.68Type I secretion system permease/ATPase n=1 Tax=Le... [more]
UPI00112DF6D03.890e-6458.19type I secretion system permease/ATPase n=1 Tax=Me... [more]
A0A3D1CKF9_9GAMM2.250e-6252.91Type I secretion system permease/ATPase (Fragment)... [more]
UPI00034CD0C32.560e-6252.33ATP-binding cassette domain-containing protein n=1... [more]
A0A1Y5DQK9_9GAMM6.890e-6155.23ABC transporter n=3 Tax=unclassified Methylophaga ... [more]
C0N3R0_9GAMM9.490e-6153.49ABC transporter, ATP-binding protein n=1 Tax=Methy... [more]
A0A661DGQ9_9GAMM1.720e-6056.40Type I secretion system permease/ATPase n=2 Tax=Ga... [more]
A0A369CBV9_9GAMM1.870e-6057.23ATP-binding cassette subfamily C protein LapB n=1 ... [more]
A0A4S1E5J8_9BURK2.250e-6054.91ATP-binding cassette domain-containing protein (Fr... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig106787contigM-pyrifera_M_contig106787:57..617 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score208.0
Seed ortholog evalue3.8e-51
Seed eggNOG ortholog1026882.MAMP_03005
Preferred namelapB
KEGG koko:K12541
KEGG TC3.A.1.109.3,3.A.1.109.4
KEGG Pathwayko02010,map02010
KEGG ModuleM00330
Hectar predicted targeting categoryother localisation
EggNOG free text desc.ABC transporter transmembrane region
EggNOG OGs1R2T0@1224,1SBE1@1236,45ZQX@72273,COG2274@1,COG2274@2
COG Functional cat.V
Best tax levelThiotrichales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko02000,ko02044
Exons1
Model size561
Cds size561
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815577.611562-CDS-M-pyrifera_M_contig106787:56..6171622815577.611562-CDS-M-pyrifera_M_contig106787:56..617Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig106787 57..617 -
1692277471.865491-CDS-M-pyrifera_M_contig106787:56..6171692277471.865491-CDS-M-pyrifera_M_contig106787:56..617Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig106787 57..617 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig106787.1455.1prot_M-pyrifera_M_contig106787.1455.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig106787 57..617 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig106787.1455.1

>prot_M-pyrifera_M_contig106787.1455.1 ID=prot_M-pyrifera_M_contig106787.1455.1|Name=mRNA_M-pyrifera_M_contig106787.1455.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=187bp
GAIRVDGTDIAQIDPADLRRQIGFVQQDPRLFYGTARENITMKAPWVTDE
EVLRVAKISGADSFIGKHPAGYDMPIGEGGQGISGGQCQAICIARSLLLS
PPIMLFDEPTSAMDNSSEQWFMENFTKAFEGKTLLLVTHKMSILGMVDRL
IVLQHGKIVADGSKERVLSALKQLQQKKSPSEVGDA*
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mRNA from alignment at M-pyrifera_M_contig106787:57..617-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig106787.1455.1 ID=mRNA_M-pyrifera_M_contig106787.1455.1|Name=mRNA_M-pyrifera_M_contig106787.1455.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=561bp|location=Sequence derived from alignment at M-pyrifera_M_contig106787:57..617- (Macrocystis pyrifera P11B4 male)
GGTGCAATACGGGTAGATGGCACTGATATAGCGCAAATTGATCCCGCTGA
CTTACGACGTCAGATTGGATTTGTGCAGCAAGATCCGCGTTTGTTTTATG
GAACAGCGCGTGAGAATATTACAATGAAAGCGCCTTGGGTGACAGATGAA
GAAGTTTTACGTGTTGCTAAGATATCAGGAGCGGACAGTTTTATTGGTAA
GCACCCGGCAGGCTACGATATGCCGATTGGTGAAGGCGGCCAAGGGATAT
CAGGCGGGCAGTGTCAAGCCATTTGTATTGCACGCTCACTTTTATTGTCT
CCACCTATCATGTTGTTTGATGAGCCAACTAGCGCAATGGATAATAGCTC
AGAACAATGGTTTATGGAAAACTTCACAAAAGCATTTGAAGGGAAAACCT
TATTACTAGTCACACATAAAATGTCTATTCTAGGTATGGTAGATCGCCTT
ATTGTTTTACAACATGGTAAAATAGTTGCTGATGGTAGCAAAGAACGTGT
ACTCAGCGCGCTTAAACAATTGCAGCAAAAAAAATCACCATCTGAAGTTG
GGGATGCATAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig106787:57..617-

>mRNA_M-pyrifera_M_contig106787.1455.1 ID=mRNA_M-pyrifera_M_contig106787.1455.1|Name=mRNA_M-pyrifera_M_contig106787.1455.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1122bp|location=Sequence derived from alignment at M-pyrifera_M_contig106787:57..617- (Macrocystis pyrifera P11B4 male)
GGTGCAATACGGGTAGATGGCACTGATATAGCGCAAATTGATCCCGCTGA
CTTACGACGTCAGATTGGATTTGTGCAGCAAGATCCGCGTTTGTTTTATG
GAACAGCGCGTGAGAATATTACAATGAAAGCGCCTTGGGTGACAGATGAA
GAAGTTTTACGTGTTGCTAAGATATCAGGAGCGGACAGTTTTATTGGTAA
GCACCCGGCAGGCTACGATATGCCGATTGGTGAAGGCGGCCAAGGGATAT
CAGGCGGGCAGTGTCAAGCCATTTGTATTGCACGCTCACTTTTATTGTCT
CCACCTATCATGTTGTTTGATGAGCCAACTAGCGCAATGGATAATAGCTC
AGAACAATGGTTTATGGAAAACTTCACAAAAGCATTTGAAGGGAAAACCT
TATTACTAGTCACACATAAAATGTCTATTCTAGGTATGGTAGATCGCCTT
ATTGTTTTACAACATGGTAAAATAGTTGCTGATGGTAGCAAAGAACGTGT
ACTCAGCGCGCTTAAACAATTGCAGCAAAAAAAATCACCATCTGAAGTTG
GGGATGCATAAGGTGCAATACGGGTAGATGGCACTGATATAGCGCAAATT
GATCCCGCTGACTTACGACGTCAGATTGGATTTGTGCAGCAAGATCCGCG
TTTGTTTTATGGAACAGCGCGTGAGAATATTACAATGAAAGCGCCTTGGG
TGACAGATGAAGAAGTTTTACGTGTTGCTAAGATATCAGGAGCGGACAGT
TTTATTGGTAAGCACCCGGCAGGCTACGATATGCCGATTGGTGAAGGCGG
CCAAGGGATATCAGGCGGGCAGTGTCAAGCCATTTGTATTGCACGCTCAC
TTTTATTGTCTCCACCTATCATGTTGTTTGATGAGCCAACTAGCGCAATG
GATAATAGCTCAGAACAATGGTTTATGGAAAACTTCACAAAAGCATTTGA
AGGGAAAACCTTATTACTAGTCACACATAAAATGTCTATTCTAGGTATGG
TAGATCGCCTTATTGTTTTACAACATGGTAAAATAGTTGCTGATGGTAGC
AAAGAACGTGTACTCAGCGCGCTTAAACAATTGCAGCAAAAAAAATCACC
ATCTGAAGTTGGGGATGCATAA
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