mRNA_M-pyrifera_M_contig101687.350.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig101687.350.1
Unique NamemRNA_M-pyrifera_M_contig101687.350.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig101687.350.1 vs. uniprot
Match: A0A831RW89_9GAMM (C-type cytochrome n=1 Tax=Thiolapillus brandeum TaxID=1076588 RepID=A0A831RW89_9GAMM)

HSP 1 Score: 210 bits (535), Expect = 2.990e-66
Identity = 107/189 (56.61%), Postives = 135/189 (71.43%), Query Frame = 1
Query:   19 LAALSIGAAAETAVGDAADPIRGAQSWADNCSRCHNMRDPSEFRDDLWRPIMTHMRMRAGLTGQQTRDILAFLQASNYRGASARPVKVATTTANPPVDAA---GGATVYNQTCVVCHGADGKGAIPGVPDFTAADGALTKSDDVLLQHIIEGFKSPEAIMMMPARGGNPALTDAELAATLAHIRANFSR 576
            L A ++G+AAET    A D  RGA SW+ NC+RCHNMR P+EFRD+LWR I+THMR+RAGLTGQQ RDILAF+QASN       P  VA+ +   PV       G  +Y  TC+ CHGADGKG +PG PDFT   G L+KSD+VLL+HI EGF+SP + M MPA+GGNP+LT +E++  L+++R  FSR
Sbjct:   12 LLAPAVGSAAETN-DKAGDANRGATSWSQNCNRCHNMRSPTEFRDELWRTIITHMRLRAGLTGQQQRDILAFMQASN------NPAPVASVSVAEPVTGGTKRSGEQIYGSTCIACHGADGKGVLPGTPDFTDKGGRLSKSDEVLLRHITEGFQSPGSPMAMPAKGGNPSLTPSEISEVLSYLRKQFSR 193          
BLAST of mRNA_M-pyrifera_M_contig101687.350.1 vs. uniprot
Match: A0A2G2HT17_9GAMM (Cytochrome c domain-containing protein n=1 Tax=Methylophaga sp. TaxID=2024840 RepID=A0A2G2HT17_9GAMM)

HSP 1 Score: 192 bits (488), Expect = 3.940e-59
Identity = 94/170 (55.29%), Postives = 120/170 (70.59%), Query Frame = 1
Query:   73 DPIRGAQSWADNCSRCHNMRDPSEFRDDLWRPIMTHMRMRAGLTGQQTRDILAFLQASNYRGASARPVKVATT--TANPPVDAAGGATVYNQTCVVCHGADGKGAIPGVPDFTAADGALTKSDDVLLQHIIEGFKSPEAIMMMPARGGNPALTDAELAATLAHIRANFSR 576
            D  RGA SWA NCSRCH MRDP EF D +WRPI+ HMR+R GLTGQQ RDIL FLQASN    +  P KVA     ++    +  G   Y+QTC+ CHGA GKG +PGVPD T ADG L+KSD+VL++HI EGF++P + M MPA+GGN  L DA++ + L+++R +F +
Sbjct:   27 DISRGAISWAQNCSRCHEMRDPKEFTDAMWRPIVAHMRVRGGLTGQQQRDILVFLQASN----NPTPKKVANNLKVSDSSKPSQSGKATYSQTCIACHGAAGKGVLPGVPDLTKADGRLSKSDEVLIKHIAEGFQTPGSPMAMPAKGGNADLNDADIKSVLSYLRESFGQ 192          
BLAST of mRNA_M-pyrifera_M_contig101687.350.1 vs. uniprot
Match: S5TFL9_9GAMM (Cytochrome c, class I n=1 Tax=Cycloclasticus zancles 78-ME TaxID=1198232 RepID=S5TFL9_9GAMM)

HSP 1 Score: 190 bits (483), Expect = 3.980e-58
Identity = 97/175 (55.43%), Postives = 122/175 (69.71%), Query Frame = 1
Query:   67 AADPIRGAQSWADNCSRCHNMRDPSEFRDDLWRPIMTHMRMRAGLTGQQTRDILAFLQASNYRGASARPVKVATTTANPPV------DAAGGATVYNQTCVVCHGADGKGAIPGVPDFTAADGALTKSDDVLLQHIIEGFKSPEAIMMMPARGGNPALTDAELAATLAHIRANFS 573
            A D  RG+++W++NC RCH+MR P+E RDD W+  + HMR+RAGLTGQ+TRDILAFLQASN   +SA      TTT  P +       A  G  +Y   C+ CHGA+GKGA+PGVPDFT   G LTK+D  LL +II GF+SP + M MPARGGN +L+DAEL+A L +IR  FS
Sbjct:   40 AGDFSRGSKAWSENCMRCHSMRSPTELRDDQWKTTVFHMRVRAGLTGQETRDILAFLQASNAPISSAN-----TTTVKPTIKKVASSSAISGEKIYQSNCMACHGANGKGALPGVPDFTNKMGPLTKTDAQLLNNIINGFQSPNSSMQMPARGGNSSLSDAELSAALGYIRETFS 209          
BLAST of mRNA_M-pyrifera_M_contig101687.350.1 vs. uniprot
Match: UPI00048BB29C (c-type cytochrome n=1 Tax=Thermithiobacillus tepidarius TaxID=929 RepID=UPI00048BB29C)

HSP 1 Score: 189 bits (479), Expect = 1.330e-57
Identity = 96/175 (54.86%), Postives = 117/175 (66.86%), Query Frame = 1
Query:   64 DAADPIRGAQSWADNCSRCHNMRDPSEFRDDLWRPIMTHMRMRAGLTGQQTRDILAFLQASN----YRGASARPVKVATTTANPPVDAAGGATVYNQTCVVCHGADGKGAIPGVPDFTAADGALTKSDDVLLQHIIEGFKSPEAIMMMPARGGNPALTDAELAATLAHIRANFSR 576
            D A   RGA +WA++C RCHNMR+P+EFRDD WR IM HMR+RAGLTGQ+TRDILAFLQASN       A   P+  A TT   P     G  VY QTC+ CHGADGKGA+PGVPD T A+G L KSD  L++ I  G + P A + MP +GGNP LT+ ++   L ++R  F R
Sbjct:   35 DTASVARGAVAWANSCDRCHNMREPAEFRDDQWRAIMAHMRVRAGLTGQETRDILAFLQASNSPAQQPSAPVAPIPTAPTTGVHP-----GKAVYTQTCLACHGADGKGALPGVPDLTRANGPLAKSDAELIRSITAGVQRPGAPLAMPPKGGNPNLTETDIRQVLDYMRQAFGR 204          
BLAST of mRNA_M-pyrifera_M_contig101687.350.1 vs. uniprot
Match: A0A2K9B1P9_9GAMM (Cytochrome c domain-containing protein n=1 Tax=Kangiella profundi TaxID=1561924 RepID=A0A2K9B1P9_9GAMM)

HSP 1 Score: 188 bits (478), Expect = 1.660e-57
Identity = 91/170 (53.53%), Postives = 115/170 (67.65%), Query Frame = 1
Query:   73 DPIRGAQSWADNCSRCHNMRDPSEFRDDLWRPIMTHMRMRAGLTGQQTRDILAFLQASNYRGAS--ARPVKVATTTANPPVDAAGGATVYNQTCVVCHGADGKGAIPGVPDFTAADGALTKSDDVLLQHIIEGFKSPEAIMMMPARGGNPALTDAELAATLAHIRANFSR 576
            D ++GAQ WADNC+RCHNMRDP E  D+ W   + HMR+RAGLTGQ  RDILAFLQASN       ++PV +     N       G  VY  +C+ CHG+DGKGA+PG PDFT A G L K+D+ LL ++I GF+SP + M MP RGGN +LTDA++ A L +IRA F +
Sbjct:   36 DAVKGAQKWADNCARCHNMRDPKELSDEYWETTVNHMRIRAGLTGQDARDILAFLQASNTPPEKEISKPVSI-----NQISSKLSGEEVYKSSCIACHGSDGKGALPGTPDFTNAKGPLNKNDEELLSNMINGFQSPGSPMAMPPRGGNASLTDADVKAVLGYIRATFGK 200          
BLAST of mRNA_M-pyrifera_M_contig101687.350.1 vs. uniprot
Match: A0A127M573_9GAMM (Cytochrome c domain-containing protein n=8 Tax=Gammaproteobacteria TaxID=1236 RepID=A0A127M573_9GAMM)

HSP 1 Score: 187 bits (475), Expect = 4.740e-57
Identity = 98/188 (52.13%), Postives = 120/188 (63.83%), Query Frame = 1
Query:   13 LTLAALSIGAAAETAVGDAADPIRGAQSWADNCSRCHNMRDPSEFRDDLWRPIMTHMRMRAGLTGQQTRDILAFLQASNYRGASARPVKVATTTANPPVDAAGGATVYNQTCVVCHGADGKGAIPGVPDFTAADGALTKSDDVLLQHIIEGFKSPEAIMMMPARGGNPALTDAELAATLAHIRANFSR 576
            L L+A  +  A   A GDA    +GAQ WADNC+RCHNMRDP E  DD W   + HMR+RAGL GQ TRDILAFLQASN          V+   AN  +    G  VY  TC+ CHG DGKGA PG+PDFT + G L+K+D  LL ++I GF+SP + M MP RGGN +LTDA+L A L ++R  F +
Sbjct:   19 LLLSATFLTPALAEAKGDAG---KGAQKWADNCARCHNMRDPKELSDDYWETTVNHMRIRAGLWGQDTRDILAFLQASNTPAKKVTVKSVSVDLANSGLS---GEAVYKSTCIACHGGDGKGAFPGIPDFTKSKGPLSKNDAELLSNMINGFQSPGSPMAMPPRGGNVSLTDADLKAVLGYMRTTFEK 200          
BLAST of mRNA_M-pyrifera_M_contig101687.350.1 vs. uniprot
Match: UPI000379810F (c-type cytochrome n=1 Tax=Kangiella aquimarina TaxID=261965 RepID=UPI000379810F)

HSP 1 Score: 186 bits (473), Expect = 9.530e-57
Identity = 91/168 (54.17%), Postives = 112/168 (66.67%), Query Frame = 1
Query:   73 DPIRGAQSWADNCSRCHNMRDPSEFRDDLWRPIMTHMRMRAGLTGQQTRDILAFLQASNYRGASARPVKVATTTANPPVDAAGGATVYNQTCVVCHGADGKGAIPGVPDFTAADGALTKSDDVLLQHIIEGFKSPEAIMMMPARGGNPALTDAELAATLAHIRANFSR 576
            D ++GAQ WADNC+RCHNMRDP E  D+ W   + HMR+RAGLTGQ  RDILAFLQASN          V+    N       G  VY  +CV CHG+DGKGA+PG PDFT A G L+K+D+ LL +II GF+SP + M MP RGGN +LTDA+L A + +IR  F +
Sbjct:   36 DAVKGAQKWADNCARCHNMRDPKELSDEYWETTVNHMRIRAGLTGQDARDILAFLQASNTPSEKEIEKSVSI---NQFKSKLSGGDVYTSSCVACHGSDGKGALPGTPDFTNAKGPLSKNDEELLSNIINGFQSPGSPMAMPPRGGNASLTDADLKAVINYIRTTFGK 200          
BLAST of mRNA_M-pyrifera_M_contig101687.350.1 vs. uniprot
Match: A0A3B0XBH5_9ZZZZ (Cytochrome c domain-containing protein n=1 Tax=hydrothermal vent metagenome TaxID=652676 RepID=A0A3B0XBH5_9ZZZZ)

HSP 1 Score: 186 bits (473), Expect = 1.080e-56
Identity = 92/185 (49.73%), Postives = 124/185 (67.03%), Query Frame = 1
Query:   28 LSIGAAAETAVGD---AADPIRGAQSWADNCSRCHNMRDPSEFRDDLWRPIMTHMRMRAGLTGQQTRDILAFLQASNYRGASARPVKVATTTANPPVDAA-GGATVYNQTCVVCHGADGKGAIPGVPDFTAADGALTKSDDVLLQHIIEGFKSPEAIMMMPARGGNPALTDAELAATLAHIRANF 570
            L + ++A +A G+     D  +GA++W+DNC+RCHN+RDP + RDD W   M HMR+RAGLTG+ TRDILAFLQASN   +    V+ +T+TA+    A   G  VY QTC+ CHG DGKG +PG PDFT   G L+K+DDVL+ H+  GF+S  + M MPA+GGN ALT  +L   L +++  F
Sbjct:   18 LVVSSSAFSAAGEWPPTGDAAKGAKTWSDNCNRCHNLRDPKQLRDDQWISTMFHMRVRAGLTGEDTRDILAFLQASNNTTSKKASVRASTSTASAEATAGLTGEGVYTQTCIACHGGDGKGTVPGAPDFTDKSGRLSKADDVLMNHMTNGFQSSGSPMAMPAKGGNSALTQGDLKNVLQYLKDTF 202          
BLAST of mRNA_M-pyrifera_M_contig101687.350.1 vs. uniprot
Match: UPI0012AEB9CE (cytochrome c n=1 Tax=Marinicella rhabdoformis TaxID=2580566 RepID=UPI0012AEB9CE)

HSP 1 Score: 184 bits (467), Expect = 8.490e-56
Identity = 88/167 (52.69%), Postives = 124/167 (74.25%), Query Frame = 1
Query:   82 RGAQSWADNCSRCHNMRDPSEFRDDLWRPIMTHMRMRAGLTGQQTRDILAFLQASNYRGASARPVKVATTT--ANPPVDAAGGATVYNQTCVVCHGADGKGAIPGVPDFTAADGALTKSDDVLLQHIIEGFKSPEAIMMMPARGGNPALTDAELAATLAHIRANFSR 576
            +GA++WA+NC+RCHNMRDP + RDD W   + HMR+RAGLTGQ+TRDIL FLQ SN      +PVK+++ +  AN P +  G   VY QTC+ CHG DGKGA+PGVPD T  +G L++S++VLL HI +GFKS  + M MPA+GGNP+L++++L   +A++++ F +
Sbjct:   41 KGAKAWAENCARCHNMRDPKDLRDDQWITTVFHMRVRAGLTGQKTRDILTFLQESNN---PPQPVKISSRSSEANNPSNMTG-KEVYEQTCIACHGKDGKGAVPGVPDLTIPEGVLSQSEEVLLAHIRDGFKSKNSPMAMPAKGGNPSLSESDLLNVVAYMKSQFKQ 203          
BLAST of mRNA_M-pyrifera_M_contig101687.350.1 vs. uniprot
Match: A0A3B0XYP1_9ZZZZ (Cytochrome c domain-containing protein n=2 Tax=hydrothermal vent metagenome TaxID=652676 RepID=A0A3B0XYP1_9ZZZZ)

HSP 1 Score: 184 bits (466), Expect = 1.540e-55
Identity = 86/164 (52.44%), Postives = 115/164 (70.12%), Query Frame = 1
Query:   85 GAQSWADNCSRCHNMRDPSEFRDDLWRPIMTHMRMRAGLTGQQTRDILAFLQASNYRGASARPVKVATTTANPPVDAAGGATVYNQTCVVCHGADGKGAIPGVPDFTAADGALTKSDDVLLQHIIEGFKSPEAIMMMPARGGNPALTDAELAATLAHIRANFSR 576
            G++ WA+NC+RCHNMR P E RDD W     HMR+RAGLTGQ+TRD++ FL+ASN +  S+  ++VA   +            Y++TC+ CHGA GKGA PG PDFT++ GAL+KSDDVL+ HI  GF+S ++ M MP RGGNP L+DA++ A LA+IR  F +
Sbjct:   52 GSKVWAENCNRCHNMRGPKELRDDQWISTSFHMRLRAGLTGQETRDVITFLKASNTKMVSSGSMQVAAVISKGET----AKETYDKTCIACHGASGKGAFPGTPDFTSSSGALSKSDDVLIDHITNGFQSADSPMAMPPRGGNPNLSDADVKAVLAYIRETFGK 211          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig101687.350.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A831RW89_9GAMM2.990e-6656.61C-type cytochrome n=1 Tax=Thiolapillus brandeum Ta... [more]
A0A2G2HT17_9GAMM3.940e-5955.29Cytochrome c domain-containing protein n=1 Tax=Met... [more]
S5TFL9_9GAMM3.980e-5855.43Cytochrome c, class I n=1 Tax=Cycloclasticus zancl... [more]
UPI00048BB29C1.330e-5754.86c-type cytochrome n=1 Tax=Thermithiobacillus tepid... [more]
A0A2K9B1P9_9GAMM1.660e-5753.53Cytochrome c domain-containing protein n=1 Tax=Kan... [more]
A0A127M573_9GAMM4.740e-5752.13Cytochrome c domain-containing protein n=8 Tax=Gam... [more]
UPI000379810F9.530e-5754.17c-type cytochrome n=1 Tax=Kangiella aquimarina Tax... [more]
A0A3B0XBH5_9ZZZZ1.080e-5649.73Cytochrome c domain-containing protein n=1 Tax=hyd... [more]
UPI0012AEB9CE8.490e-5652.69cytochrome c n=1 Tax=Marinicella rhabdoformis TaxI... [more]
A0A3B0XYP1_9ZZZZ1.540e-5552.44Cytochrome c domain-containing protein n=2 Tax=hyd... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig101687contigM-pyrifera_M_contig101687:12..590 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score193.0
Seed ortholog evalue1.3e-46
Seed eggNOG ortholog1198232.CYCME_1313
Preferred namesoxX
KEGG rclassRC02794,RC03151,RC03152
KEGG koko:K02305,ko:K17223
KEGG TC3.D.4.10
KEGG ReactionR00294,R10151
KEGG Pathwayko00910,ko00920,ko01100,ko01120,map00910,map00920,map01100,map01120
KEGG ModuleM00529,M00595
Hectar predicted targeting categorysignal peptide
EggNOG free text desc.Cytochrome c
EggNOG OGs1RFJV@1224,1S4GY@1236,COG2010@1,COG2010@2
COG Functional cat.C
Best tax levelGammaproteobacteria
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002
Exons1
Model size579
Cds size579
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815515.3408456-CDS-M-pyrifera_M_contig101687:11..5901622815515.3408456-CDS-M-pyrifera_M_contig101687:11..590Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig101687 12..590 +
1692277445.9726648-CDS-M-pyrifera_M_contig101687:11..5901692277445.9726648-CDS-M-pyrifera_M_contig101687:11..590Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig101687 12..590 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig101687.350.1prot_M-pyrifera_M_contig101687.350.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig101687 12..590 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig101687.350.1

>prot_M-pyrifera_M_contig101687.350.1 ID=prot_M-pyrifera_M_contig101687.350.1|Name=mRNA_M-pyrifera_M_contig101687.350.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=193bp
VLLLLTLAALSIGAAAETAVGDAADPIRGAQSWADNCSRCHNMRDPSEFR
DDLWRPIMTHMRMRAGLTGQQTRDILAFLQASNYRGASARPVKVATTTAN
PPVDAAGGATVYNQTCVVCHGADGKGAIPGVPDFTAADGALTKSDDVLLQ
HIIEGFKSPEAIMMMPARGGNPALTDAELAATLAHIRANFSR*
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mRNA from alignment at M-pyrifera_M_contig101687:12..590+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig101687.350.1 ID=mRNA_M-pyrifera_M_contig101687.350.1|Name=mRNA_M-pyrifera_M_contig101687.350.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=579bp|location=Sequence derived from alignment at M-pyrifera_M_contig101687:12..590+ (Macrocystis pyrifera P11B4 male)
GTTTTACTGTTGTTGACGCTCGCGGCGCTTTCCATCGGCGCGGCGGCCGA GACTGCGGTGGGCGACGCAGCCGACCCGATCCGTGGTGCGCAAAGCTGGG CAGACAACTGCTCACGCTGTCACAACATGCGCGATCCGAGCGAGTTTCGC GACGATCTGTGGCGCCCCATCATGACGCATATGCGAATGCGGGCCGGGCT GACGGGTCAGCAGACGCGCGACATCCTGGCCTTTCTGCAGGCGAGCAATT ACCGCGGCGCGAGCGCACGCCCGGTTAAAGTTGCAACAACAACGGCGAAC CCGCCTGTGGACGCTGCTGGCGGCGCAACGGTTTACAACCAAACCTGCGT CGTGTGTCACGGTGCCGACGGCAAAGGCGCAATTCCGGGTGTTCCGGACT TTACTGCTGCCGACGGTGCGCTTACGAAGTCGGATGACGTGTTGCTGCAG CACATCATTGAAGGATTCAAGAGCCCGGAAGCGATCATGATGATGCCCGC CCGCGGCGGCAATCCGGCACTCACCGACGCCGAGCTTGCAGCGACACTCG CACATATTCGGGCAAACTTCTCGCGTTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig101687:12..590+

>mRNA_M-pyrifera_M_contig101687.350.1 ID=mRNA_M-pyrifera_M_contig101687.350.1|Name=mRNA_M-pyrifera_M_contig101687.350.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1158bp|location=Sequence derived from alignment at M-pyrifera_M_contig101687:12..590+ (Macrocystis pyrifera P11B4 male)
GTTTTACTGTTGTTGACGCTCGCGGCGCTTTCCATCGGCGCGGCGGCCGA
GACTGCGGTGGGCGACGCAGCCGACCCGATCCGTGGTGCGCAAAGCTGGG
CAGACAACTGCTCACGCTGTCACAACATGCGCGATCCGAGCGAGTTTCGC
GACGATCTGTGGCGCCCCATCATGACGCATATGCGAATGCGGGCCGGGCT
GACGGGTCAGCAGACGCGCGACATCCTGGCCTTTCTGCAGGCGAGCAATT
ACCGCGGCGCGAGCGCACGCCCGGTTAAAGTTGCAACAACAACGGCGAAC
CCGCCTGTGGACGCTGCTGGCGGCGCAACGGTTTACAACCAAACCTGCGT
CGTGTGTCACGGTGCCGACGGCAAAGGCGCAATTCCGGGTGTTCCGGACT
TTACTGCTGCCGACGGTGCGCTTACGAAGTCGGATGACGTGTTGCTGCAG
CACATCATTGAAGGATTCAAGAGCCCGGAAGCGATCATGATGATGCCCGC
CCGCGGCGGCAATCCGGCACTCACCGACGCCGAGCTTGCAGCGACACTCG
CACATATTCGGGCAAACTTCTCGCGTTGAGTTTTACTGTTGTTGACGCTC
GCGGCGCTTTCCATCGGCGCGGCGGCCGAGACTGCGGTGGGCGACGCAGC
CGACCCGATCCGTGGTGCGCAAAGCTGGGCAGACAACTGCTCACGCTGTC
ACAACATGCGCGATCCGAGCGAGTTTCGCGACGATCTGTGGCGCCCCATC
ATGACGCATATGCGAATGCGGGCCGGGCTGACGGGTCAGCAGACGCGCGA
CATCCTGGCCTTTCTGCAGGCGAGCAATTACCGCGGCGCGAGCGCACGCC
CGGTTAAAGTTGCAACAACAACGGCGAACCCGCCTGTGGACGCTGCTGGC
GGCGCAACGGTTTACAACCAAACCTGCGTCGTGTGTCACGGTGCCGACGG
CAAAGGCGCAATTCCGGGTGTTCCGGACTTTACTGCTGCCGACGGTGCGC
TTACGAAGTCGGATGACGTGTTGCTGCAGCACATCATTGAAGGATTCAAG
AGCCCGGAAGCGATCATGATGATGCCCGCCCGCGGCGGCAATCCGGCACT
CACCGACGCCGAGCTTGCAGCGACACTCGCACATATTCGGGCAAACTTCT
CGCGTTGA
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