mRNA_L-elsbetiae_contig8642.17678.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig8642.17678.1
Unique NamemRNA_L-elsbetiae_contig8642.17678.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
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BLAST of mRNA_L-elsbetiae_contig8642.17678.1 vs. uniprot
Match: A0A6H5JK53_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JK53_9PHAE)

HSP 1 Score: 105 bits (262), Expect = 7.510e-21
Identity = 108/336 (32.14%), Postives = 150/336 (44.64%), Query Frame = 2 
Query:  404 QAGADLTPRDRAGTTPLLAALIIRYDGGTPGLDMVQALLRRGANPLLANDQRLAPMNQAAGHGDTGMVDMILSAAPAALNLGTGKQGTTAMGITVDSRVLSPHDKKRTVRHLLSLGATDRDVPENVASTLVGAVRRGDNSMVRLLLDAARQEVGDFWGADAVYLAIIVAAGNGLAKILEMLITAEGEERQQRWTRVRV-SGVPAVFRRGCEPLCLDDRRPSRGRRGRAGPRRRGLLAPS----SMRQQGSQPKTSTQTTARHPPSGGRWRVRPLSGPVPGRGRLR*YLHL*PVVLVLLLLPGGGLRKSSTASTGVRIFRPKDKRFFTTRFGRYSRK 1396
            +AGAD   RD AG TPL+   +        G  + + L + GA+P + N   ++ M+ AAG G+  ++ +IL+  P+ALN+   K G T MGI       +   ++R V HLLS GA+D  V E  A+ L  A   G   MVR++L+     VG   G  A+  +I VA     AKILEML++AEGEE +++W   R+  G P         +       S G R    P   G LA      ++R    +         R    G  +R R    P    G     + L               R     S GV+IFRP   RFF TRF RY+ K
Sbjct:   96 EAGADFNSRDGAGETPLMLTAL-----ADTGALLARELFKVGASPFVKNADGMSAMHVAAGLGNLEVMTVILTNTPSALNVLDNK-GFTPMGIA------ALRGQRRAVFHLLSAGASDLGVDEQNAAIL-WACDGGHPRMVRMMLEHGINRVG---GLGAIPNSIGVAVQGRQAKILEMLVSAEGEENRRKWAECRLRDGTPLHLAAAWVSVRAIHVLLSAGARETL-PNGYGKLASEEVGIALRPPARRSPRKEAAVVRMLQRGPAYRARSYGWPTLDGGNGTSDVTL-------------SSRTRGANSFGVQIFRPTGTRFFATRFARYANK 401
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BLAST of mRNA_L-elsbetiae_contig8642.17678.1 vs. uniprot
Match: D7G4M4_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G4M4_ECTSI)

HSP 1 Score: 102 bits (254), Expect = 1.390e-19
Identity = 110/341 (32.26%), Postives = 154/341 (45.16%), Query Frame = 2 
Query:  404 QAGADLTPRDRAGTTPLLAALIIRYDGGTPGLDMVQALLRRGANPLLANDQRLAPMNQAAGHGDTGMVDMILSAAPAALNLGTGKQGTTAMGITVDSRVLSPHDKKRTVRHLLSLGATDRDVPENVASTLVGAVRRGDNSMVRLLLDAARQEVGDFWGADAVYLAIIVAAGNGLAKILEMLITAEGEERQQRWTRVRV-SGVPAVFRRGCEPLCLDDRRPSRGRRGRAGPRRRGLLAPSSMRQQGSQPKTSTQTTARHPPSGGRWRVRPLSGPVPGRGRLR*YLHL*PVVLVLLLLPGGGLRKSSTA---------STGVRIFRPKDKRFFTTRFGRYSRK 1396
            +AGAD   RD AG TPL+   +        G  + + L + GA+P + N   ++ M+ AAG G+  ++ +IL+  P+ LN+   K G T MGI       +   ++R V HLLS GA+D  V E  A+ +  A   G   MVR++L+     VG   G  A+  +I VA     AKILEML++AEGE+ +++W   R+  G P         + +     S G R    P R G LA       G +   +    AR  P      VR L      R R   +  L            GG   S  A         S GV+I+RP   RFF TRF RY+ K
Sbjct:  151 EAGADFNSRDGAGETPLMLTAL-----ADTGALLARELFKVGASPFVKNADGMSAMHVAAGLGNLEVMTVILTNTPSVLNVLDNK-GFTPMGIA------ALRGQRRAVFHLLSAGASDLGVDEQNAA-IFWACDGGHPRMVRMMLEHGINRVG---GLGAIPNSIGVAVQGRQAKILEMLVSAEGEDNRRKWAECRLRDGTPLHLAAAWVSVRVMHVLLSAGARETL-PNRYGKLA-------GEEVGIALGPPARRSPRKEAAVVRMLQRGPAYRARSYGWPTL-----------DGGNGTSDVALSSRTRGAKSFGVQIYRPTGTRFFATRFARYANK 456
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BLAST of mRNA_L-elsbetiae_contig8642.17678.1 vs. uniprot
Match: D8LSK4_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LSK4_ECTSI)

HSP 1 Score: 74.3 bits (181), Expect = 1.570e-10
Identity = 67/213 (31.46%), Postives = 105/213 (49.30%), Query Frame = 2 
Query:  392 RCFAQAGADLTPRDRAGTTPLLAALIIRYDGGTPGLDMVQALLRRGANPLLANDQRLAPMNQAAGHGDTGMVDMILSAAP-AALNLGTGKQGTTAMGITVDSRVLSPHDKKRTVRHLLSLGATDRDVPE-NVASTLVGAVRRGDNSMVRLLL-DAARQEVGDFWGADAVYLAIIVAAGNGLAKILEMLITAEGEERQQRWTRVR-VSGVPAVF 1018
            R   +AGADL  R+  G TPL+ A  +  +G    +++ + LL+ G +  L +    A ++ AA  G+  ++  +LS    + LNL T + G T + + V+            V  LLS GA+ R + E N       A   G  S++R+L  +     VG   GA  +  ++  A   G A+IL +L+ AEG+ERQ+ W R R V G P V+
Sbjct:   99 RELIKAGADLNGRNYQGCTPLMMACRVDKEGAER-VELARELLKAGVDTTLVDADGRAALHYAAYSGNEALIQALLSKGSLSTLNLAT-QDGKTPLCVAVEFVHCG------VVSLLLSAGASQRGLQEGNYPCPFRSAAALGYESILRILCSERGMDAVGG--GAAVLPNSLACATRRGKARILRLLLAAEGQERQEHWARRRLVLGRPLVY 301
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig8642.17678.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 3
Match NameE-valueIdentityDescription
A0A6H5JK53_9PHAE7.510e-2132.14Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7G4M4_ECTSI1.390e-1932.26Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
D8LSK4_ECTSI1.570e-1031.46Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig8642contigL-elsbetiae_contig8642:2224..5054 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score83.2
Seed ortholog evalue1.9e-13
Seed eggNOG ortholog2880.D7G4M4
KEGG koko:K10324,ko:K21435
Hectar predicted targeting categoryother localisation
GOsGO:0001654,GO:0001736,GO:0001737,GO:0001738,GO:0001745,GO:0002009,GO:0002165,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0007163,GO:0007164,GO:0007275,GO:0007399,GO:0007423,GO:0007444,GO:0007472,GO:0007476,GO:0007552,GO:0007560,GO:0008150,GO:0008544,GO:0009653,GO:0009791,GO:0009886,GO:0009887,GO:0009888,GO:0009913,GO:0009987,GO:0016043,GO:0022008,GO:0030030,GO:0030154,GO:0030182,GO:0030855,GO:0032501,GO:0032502,GO:0035107,GO:0035114,GO:0035120,GO:0035220,GO:0035239,GO:0035295,GO:0035315,GO:0035316,GO:0035317,GO:0042067,GO:0044424,GO:0044464,GO:0048513,GO:0048563,GO:0048569,GO:0048592,GO:0048699,GO:0048707,GO:0048729,GO:0048731,GO:0048736,GO:0048737,GO:0048749,GO:0048856,GO:0048869,GO:0060429,GO:0060562,GO:0071840,GO:0090596,GO:0120036
EggNOG free text desc.response to abiotic stimulus
EggNOG OGsCOG0666@1,KOG0504@2759
COG Functional cat.G
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03036,ko04121
Exons3
Model size1668
Cds size744
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig8642.17678.1prot_L-elsbetiae_contig8642.17678.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig8642 3604..4552 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816946.1873288-UTR-L-elsbetiae_contig8642:2223..25081622816946.1873288-UTR-L-elsbetiae_contig8642:2223..2508Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig8642 2224..2508 -
1691683932.3022394-UTR-L-elsbetiae_contig8642:2223..25081691683932.3022394-UTR-L-elsbetiae_contig8642:2223..2508Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig8642 2224..2508 -
1622816946.1986144-UTR-L-elsbetiae_contig8642:3466..36031622816946.1986144-UTR-L-elsbetiae_contig8642:3466..3603Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig8642 3467..3603 -
1691683932.319014-UTR-L-elsbetiae_contig8642:3466..36031691683932.319014-UTR-L-elsbetiae_contig8642:3466..3603Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig8642 3467..3603 -
1622816946.2343123-UTR-L-elsbetiae_contig8642:4552..50541622816946.2343123-UTR-L-elsbetiae_contig8642:4552..5054Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig8642 4553..5054 -
1691683932.3629417-UTR-L-elsbetiae_contig8642:4552..50541691683932.3629417-UTR-L-elsbetiae_contig8642:4552..5054Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig8642 4553..5054 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816946.2098832-CDS-L-elsbetiae_contig8642:3603..40291622816946.2098832-CDS-L-elsbetiae_contig8642:3603..4029Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig8642 3604..4029 -
1691683932.3387907-CDS-L-elsbetiae_contig8642:3603..40291691683932.3387907-CDS-L-elsbetiae_contig8642:3603..4029Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig8642 3604..4029 -
1622816946.2218266-CDS-L-elsbetiae_contig8642:4234..45521622816946.2218266-CDS-L-elsbetiae_contig8642:4234..4552Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig8642 4235..4552 -
1691683932.3517988-CDS-L-elsbetiae_contig8642:4234..45521691683932.3517988-CDS-L-elsbetiae_contig8642:4234..4552Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig8642 4235..4552 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig8642.17678.1

>prot_L-elsbetiae_contig8642.17678.1 ID=prot_L-elsbetiae_contig8642.17678.1|Name=mRNA_L-elsbetiae_contig8642.17678.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=248bp
MVQALLRRGANPLLANDQRLAPMNQAAGHGDTGMVDMILSAAPAALNLGT
GKQGTTAMGITVDSRVLSPHDKKRTVRHLLSLGATDRDVPENVASTLVGA
VRRGDNSMVRLLLDAARQEVGDFWGADAVYLAIIVAAGNGLAKILEMLIT
AEGEERQQRWTRVRVSGVPAVFRRGCEPLCLDDRRPSRGRRGRAGPRRRG
LLAPSSMRQQGSQPKTSTQTTARHPPSGGRWRVRPLSGPVPGRGRLR*
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mRNA from alignment at L-elsbetiae_contig8642:2224..5054-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig8642.17678.1 ID=mRNA_L-elsbetiae_contig8642.17678.1|Name=mRNA_L-elsbetiae_contig8642.17678.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=2831bp|location=Sequence derived from alignment at L-elsbetiae_contig8642:2224..5054- (Laminarionema elsbetiae ELsaHSoW15)
CAGGAGAGGCGCGAATATGGAGGCCGCTGTGCCTCGAGACGTGCCTGGTG
GATGGGAAGCGCAACCTTTACCGAAAGGGGCGCGAACGCTGCACGCCGCC
GTGGCGAGCGGAAGCTAGGGGCCGTACAGGCGGTGCTCGAAGCAGGAGTT
CGCTTTGATTGTCAGCACGCTCCCGCAGAGCCGGAACCAGAAGAGTGCTT
GCTCCTCTCGTGCTTGGCGCCTTTGCGCAACGTAGACGTGCCGATTCTTA
GAACCTCTGTGCGTACATACTGCGACGCTCGCAGAGGGGGTGCGCCCTTG
CGGGTGTGCTCGAAACGGCGCCCCGGACTTATTTGCCCCATCTGGGGGAA
GCCCTCGCATTGCCAGTAACGATAACCATGGATGACACCATCGCTGTTTT
GCGCAAGCAGGCGCAGACCTTACCCCGCGGGACAGGGCAGGCACCACGCC
GCTTCTCGCGGCCCTCATCATCCGGTACGATGGAGGTACGCCGGGGTTGG
ACATGGTGCAAGCGCTGCTGAGGCGGGGTGCCAACCCTCTGCTGGCCAAC
GACCAAAGGCTCGCTCCGATGAACCAGGCCGCTGGCCACGGAGACACTGG
CATGGTCGACATGATACTCTCTGCGGCACCGGCGGCGCTCAACCTGGGAA
CCGGCAAGCAGGGCACTACCGCCATGGGCATAACGGTGGATTCGCGTGTG
CTCTCTCCCCACGACAAGAAGAGGACAGTGCGCCATCTTCTTTCGCTGGG
GGCCACTGACAGGGACGTGCCGGAGAATGTGGCAAGTACGCTCGTCGGAG
CGGTGCGTCGAGGCGACAACGTGAGTTCGTATTTTTTGCTACCGCCGCTT
CGCAATAGTACTTGGTACGCATGTTTGTGTGCAACTGCTCTTCATGAAAT
CGTCATCAGCCCATACAGTTGCCCGGCGCTTCTACATACACCCCGCGGCA
AAAGCAAATCGAAAGCGAGCGTTTCCATGGCGAACCCCGCATTGATGCTC
CCGTGCGTTTTCGCATGCTTTGCAGAGCATGGTACGCCTACTGCTGGACG
CAGCGCGGCAAGAGGTGGGAGATTTTTGGGGTGCAGACGCGGTCTATTTG
GCCATTATCGTGGCAGCCGGGAACGGGCTTGCCAAGATCCTCGAGATGCT
CATCACCGCCGAGGGCGAAGAACGGCAACAACGTTGGACGAGAGTGCGCG
TGAGCGGCGTGCCGGCCGTTTTTCGGCGCGGCTGCGAGCCGCTCTGCCTC
GACGACCGGCGTCCTTCTCGCGGCCGGCGCGGACGAGCAGGACCTCGACG
ACGAGGGCTACTTGCGCCATCGAGTATGCGGCAACAAGGCTCCCAGCCGA
AAACATCAACGCAGACAACAGCAAGACATCCGCCGTCAGGCGGACGCTGG
AGAGTGCGCCCGCTTTCCGGGCCCGTTCCTGGGCGTGGTCGGCTGCGGTG
ATATCTTCATTTATAGCCGGTTGTGCTGGTGCTTCTGTTACTGCCGGGGG
GGGGGCTTCGTAAGTCGAGTACGGCCTCTACAGGTGTCCGCATCTTCCGG
CCGAAGGACAAAAGGTTTTTCACGACCCGGTTTGGCAGGTGGGGTGGGTG
GGGCACAAGGTGAAAAAAACCTTGTAGGTGTTGATCTTCGCACTCTTGGC
TTTGGTCGTGCGTATCCAACGCGCAGTATGTCCGAGTAGGATCGTGGTAA
ACCGTCTGCCCTGCCCAGGTTAGAAGTGTTGGGGTCACCAAACAATCTAT
AATTTTGCGGGCGTCGTGTGCTCAGCGCCGGTTTGATTGTCCCGAACGGA
AAAGGGGGGTGGTAGAACCTCGTCTCCTGCAAGGTGCCGAAAACGCGTTG
TTGTTCAACGGCATCCGTTCGGGTTTCGTTAGCATGTTTTTTTGCCTTTC
ATCCGCACACTTCCAACCGGTGCGGACGTCCTTGATGTTCCCCCCGCCAT
TTTACTCAACGCGCGCAAGTATACTCTGTAATGGCGCCATAGTTACATAT
TGAGATGTCGATCAGGCTAGAACTGCCTCGGCCCTTCACAACCAAGGTTG
AAATATTTGCTGCGGATGCATGTTGCACTTACCTTGATTCGCCTCCCCCA
TCCGCGGGCGCGTGCATGCCGTAGGTGACAAAAGATAAGACACCCCCTGG
ACTGATTTTCAAAGCTCAAGTACTCCACAGCAAGCAGAGCGTCAGTCCCG
AAAAGGGCGGCGCTGGAAAGATCGCGTCGAGAGCTGTCCGAAAATGTGTC
CGTGTGGTATTAGAATCGTATTCGTTGCGGAGTAACCAAGCTTTCAATCT
CGGTCCAGAGGGTGTCTTATCTTATGGCATAGTAATATACGGTACACGCC
CGGAGTGAGGTACTGCGAGGCTTGGTATTTCACATGCCTGCCGCCGCCTG
CTGTTGCACCGTTGTGGATGCTACACATGTCAGGCTACCGGCTGACGCAA
AGCGAAATCGTTTTCTCTGCTACTCAAAAGTCGCTTTTCTATTTGAGTCG
CATATATTGGTGCGCGGTGTTACCTCTTGCTCGGTACCGTCTCCAGGTAT
TCTCGCAAGTTCTGAGGGGCTGACGAAGACGTCAGCAAACTCTGGGCGAC
GAAAATCTTGTGCGGTGTACTTAGACTATGTACGTAGTGCTCGATGTGCG
GTTGGTGAGAGGCTCGTTGGCGCCATGTGGTGGTGGCGGGGCGTTGCTGT
ACTTGTGCTGCTTTAGGTGGAAGCGCTCTGTGACCCAGCGATGAGCATCT
TCCTCCCTGTTTTGGTTGCATTTTTGTCCACCACGGTTGTATGCGGTAGA
CTCGGCTCGATTGGAGGAGTGTCACGTAGGG
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Coding sequence (CDS) from alignment at L-elsbetiae_contig8642:2224..5054-

>mRNA_L-elsbetiae_contig8642.17678.1 ID=mRNA_L-elsbetiae_contig8642.17678.1|Name=mRNA_L-elsbetiae_contig8642.17678.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=1488bp|location=Sequence derived from alignment at L-elsbetiae_contig8642:2224..5054- (Laminarionema elsbetiae ELsaHSoW15)
ATGGTGCAAGCGCTGCTGAGGCGGGGTGCCAACCCTCTGCTGGCCAACGA
CCAAAGGCTCGCTCCGATGAACCAGGCCGCTGGCCACGGAGACACTGGCA
TGGTCGACATGATACTCTCTGCGGCACCGGCGGCGCTCAACCTGGGAACC
GGCAAGCAGGGCACTACCGCCATGGGCATAACGGTGGATTCGCGTGTGCT
CTCTCCCCACGACAAGAAGAGGACAGTGCGCCATCTTCTTTCGCTGGGGG
CCACTGACAGGGACGTGCCGGAGAATGTGGCAAGTACGCTCGTCGGAGCG
GTGCGTCGAGGCGACAACATGGTGCAAGCGCTGCTGAGGCGGGGTGCCAA
CCCTCTGCTGGCCAACGACCAAAGGCTCGCTCCGATGAACCAGGCCGCTG
GCCACGGAGACACTGGCATGGTCGACATGATACTCTCTGCGGCACCGGCG
GCGCTCAACCTGGGAACCGGCAAGCAGGGCACTACCGCCATGGGCATAAC
GGTGGATTCGCGTGTGCTCTCTCCCCACGACAAGAAGAGGACAGTGCGCC
ATCTTCTTTCGCTGGGGGCCACTGACAGGGACGTGCCGGAGAATGTGGCA
AGTACGCTCGTCGGAGCGGTGCGTCGAGGCGACAACAGCATGGTACGCCT
ACTGCTGGACGCAGCGCGGCAAGAGGTGGGAGATTTTTGGGGTGCAGACG
CGGTCTATTTGGCCATTATCGTGGCAGCCGGGAACGGGCTTGCCAAGATC
CTCGAGATGCTCATCACCGCCGAGGGCGAAGAACGGCAACAACGTTGGAC
GAGAGTGCGCGTGAGCGGCGTGCCGGCCGTTTTTCGGCGCGGCTGCGAGC
CGCTCTGCCTCGACGACCGGCGTCCTTCTCGCGGCCGGCGCGGACGAGCA
GGACCTCGACGACGAGGGCTACTTGCGCCATCGAGTATGCGGCAACAAGG
CTCCCAGCCGAAAACATCAACGCAGACAACAGCAAGACATCCGCCGTCAG
GCGGACGCTGGAGAGTGCGCCCGCTTTCCGGGCCCGTTCCTGGGCGTGGT
CGGCTGCGGTGAAGCATGGTACGCCTACTGCTGGACGCAGCGCGGCAAGA
GGTGGGAGATTTTTGGGGTGCAGACGCGGTCTATTTGGCCATTATCGTGG
CAGCCGGGAACGGGCTTGCCAAGATCCTCGAGATGCTCATCACCGCCGAG
GGCGAAGAACGGCAACAACGTTGGACGAGAGTGCGCGTGAGCGGCGTGCC
GGCCGTTTTTCGGCGCGGCTGCGAGCCGCTCTGCCTCGACGACCGGCGTC
CTTCTCGCGGCCGGCGCGGACGAGCAGGACCTCGACGACGAGGGCTACTT
GCGCCATCGAGTATGCGGCAACAAGGCTCCCAGCCGAAAACATCAACGCA
GACAACAGCAAGACATCCGCCGTCAGGCGGACGCTGGAGAGTGCGCCCGC
TTTCCGGGCCCGTTCCTGGGCGTGGTCGGCTGCGGTGA
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