mRNA_L-elsbetiae_contig5885.14347.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig5885.14347.1
Unique NamemRNA_L-elsbetiae_contig5885.14347.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
BLAST of mRNA_L-elsbetiae_contig5885.14347.1 vs. uniprot
Match: A0A6H5KXK5_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KXK5_9PHAE)

HSP 1 Score: 68.2 bits (165), Expect = 3.990e-8
Identity = 35/66 (53.03%), Postives = 49/66 (74.24%), Query Frame = 3
Query: 1869 MVDYLLKPGADETILNDECKAAADVVGEDVEEEIQVAEDIERIRKLLPNAPADRA*FFGQRTSYML 2066
            +V+ LL+ GADETI N +   A D++GEDVEEE ++ ED+ER+R+LL NAPADRA    +R  Y++
Sbjct:  262 VVNSLLRAGADETIANVQGNKAVDMIGEDVEEEDRLTEDVERVRELLANAPADRA---WRRRGYLI 324          
BLAST of mRNA_L-elsbetiae_contig5885.14347.1 vs. uniprot
Match: A0A851Y5Z7_EOLRO (ASB3 protein (Fragment) n=1 Tax=Eolophus roseicapilla TaxID=176039 RepID=A0A851Y5Z7_EOLRO)

HSP 1 Score: 62.8 bits (151), Expect = 1.100e-6
Identity = 32/78 (41.03%), Postives = 49/78 (62.82%), Query Frame = 1
Query: 1219 LHVAIQSGHGDMVNDLLENGASIDAEDTSLRGYTPLQLAARMRKTEMVQLLLLKGAGKDALDSKNRTPLYLAVLYGNV 1452
            L +A+++GH D V  LL++GA++     SL G+  L  A+    TE++Q+LL KGAGK+  D    TPL++A  YG +
Sbjct:  116 LFLAVENGHADTVTFLLQHGANVQGPH-SLSGWNSLHQASLQECTEIMQMLLEKGAGKECKDDFGITPLFVAAQYGKL 192          
BLAST of mRNA_L-elsbetiae_contig5885.14347.1 vs. uniprot
Match: UPI001E60D468 (ankyrin repeat domain-containing protein n=1 Tax=Nostoc sp. CHAB 5715 TaxID=2780400 RepID=UPI001E60D468)

HSP 1 Score: 62.8 bits (151), Expect = 1.740e-6
Identity = 36/87 (41.38%), Postives = 52/87 (59.77%), Query Frame = 2
Query: 1466 LLAAGADVGLRCTQYKTSVVHIAAEGGLVDIMRAAIERGADVDAVDTADQATPLYRATVFSEVGAINVRLGAGANIEARDRYGRTAL 1726
            L+ AG D+   C   KT V+H+AA  G + ++    E GAD+D +D +  +TPL       +V AI V + +GAN+ AR+RYG TAL
Sbjct:  254 LIEAGMDINAFCDTGKT-VIHLAACWGSITMIELLAELGADLDILDESRDSTPLMMTVTRRDVRAIQVLIRSGANVNARNRYGHTAL 339          
BLAST of mRNA_L-elsbetiae_contig5885.14347.1 vs. uniprot
Match: A0A5N5P4Y9_9ROSI (Uncharacterized protein n=1 Tax=Salix brachista TaxID=2182728 RepID=A0A5N5P4Y9_9ROSI)

HSP 1 Score: 61.2 bits (147), Expect = 1.770e-6
Identity = 33/73 (45.21%), Postives = 47/73 (64.38%), Query Frame = 1
Query: 1216 SLHVAIQSGHGDMVNDLLENGASIDAEDTSLRGYTPLQLAARMRKTEMVQLLLLKGA--GKDALDSKNRTPLY 1428
            +LH+A Q GH D++++LLE GA+IDA    + G+TPL  AA+ RK E V+ L+  GA    D  DS+   PL+
Sbjct:  136 ALHLAAQGGHLDIMDELLERGANIDARTLGVCGWTPLHRAAKERKKEAVKFLIENGAFLPDDMNDSRFNPPLH 208          
BLAST of mRNA_L-elsbetiae_contig5885.14347.1 vs. uniprot
Match: A0A401PMR3_SCYTO (Uncharacterized protein n=1 Tax=Scyliorhinus torazame TaxID=75743 RepID=A0A401PMR3_SCYTO)

HSP 1 Score: 56.2 bits (134), Expect = 2.530e-6
Identity = 33/69 (47.83%), Postives = 38/69 (55.07%), Query Frame = 1
Query: 1252 MVNDLLENGASIDAEDTSLRGYTPLQLAARMRKTEMVQLLLLKGAGKDALDSKNRTPLYLAVLYGNVAA 1458
            M+  LLE  A+I+A  T     TP   AA+   T  +Q L  KGA    LD K RTPLYLA LYGN  A
Sbjct:    1 MIELLLEKHANIEARTTE-EWQTPFHYAAKYDATNSLQCLHNKGANIHVLDYKQRTPLYLAALYGNCQA 68          
BLAST of mRNA_L-elsbetiae_contig5885.14347.1 vs. uniprot
Match: A0A6H5JHY9_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JHY9_9PHAE)

HSP 1 Score: 61.2 bits (147), Expect = 1.130e-5
Identity = 33/59 (55.93%), Postives = 42/59 (71.19%), Query Frame = 3
Query:  612 AANAGKQEAAEAVDLLLRSGADETILNDDGEAAADVVAEYVEESDQVTEDIERVHELLA 788
            AA AG    AE VDLLLR GADET ++++G  AAD+V    EESD + ED++RV +LLA
Sbjct:  630 AAKAGSPGTAEMVDLLLRRGADETAVSENGHTAADMVVLKTEESDSLAEDVDRVRKLLA 688          
BLAST of mRNA_L-elsbetiae_contig5885.14347.1 vs. uniprot
Match: D7FQ25_ECTSI (Similar to ankyrin 2,3/unc44 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FQ25_ECTSI)

HSP 1 Score: 60.5 bits (145), Expect = 1.570e-5
Identity = 32/61 (52.46%), Postives = 43/61 (70.49%), Query Frame = 3
Query:  612 AANAGKQEAAEAVDLLLRSGADETILNDDGEAAADVVAEYVEESDQVTEDIERVHELLATL 794
            AA AG    AE VDLLLR GADET ++++G  AAD+V    EESD + ED++RV +LL ++
Sbjct:  467 AAKAGSPGTAEMVDLLLRRGADETAVSENGHTAADMVGLKTEESDSLAEDVDRVRKLLGSV 527          
BLAST of mRNA_L-elsbetiae_contig5885.14347.1 vs. uniprot
Match: A0A6H5JMJ6_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JMJ6_9PHAE)

HSP 1 Score: 57.4 bits (137), Expect = 1.690e-5
Identity = 97/147 (65.99%), Postives = 109/147 (74.15%), Query Frame = 3
Query:  345 AAIERGADVHAVDTAQVTPLLYAAWHNKVEAINVLVKAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRAAANAGKQEAAEAVDLLLRSGADETILNDDGEAAADVVAEYVEESDQVTEDIERVHELL 785
            AAIERGA+V A D  + TP  +AA  N VE I+VLV  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX  A A AG +   E VD LLRSGADETIL+ +G AA D+++  V       E  +RV +LL
Sbjct:    3 AAIERGAEVDAADAVERTPPHHAALLNNVEEIDVLVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRSATAVAGFEGDTEVVDSLLRSGADETILDSEGRAAVDMISWEVAGLHLSGEQDDRVRDLL 149          
BLAST of mRNA_L-elsbetiae_contig5885.14347.1 vs. uniprot
Match: V3ZWH9_LOTGI (Uncharacterized protein n=1 Tax=Lottia gigantea TaxID=225164 RepID=V3ZWH9_LOTGI)

HSP 1 Score: 54.7 bits (130), Expect = 3.020e-5
Identity = 28/76 (36.84%), Postives = 44/76 (57.89%), Query Frame = 1
Query:   31 VNDLLENEASVASKDTNGSTPLHMAAEEGKTEVVELLLLKGEEEDGLDNGGWTPLCMAVWYGHLAAALALLAADAD 258
            V   LE +  + ++  +G   +H AA+ G+ E++E L+ KG + + LD+ G TPLC AV+ GH+     LL   AD
Sbjct:   16 VKTALEEQKGLQNETISGRKLIHFAADYGQPEMIEFLVHKGADVNALDSHGITPLCAAVFEGHVKCVKILLEKGAD 91          
BLAST of mRNA_L-elsbetiae_contig5885.14347.1 vs. uniprot
Match: A0A8K0SY76_9HYPO (Putative multiple ankyrin repeats single kh domain protein n=1 Tax=Stachybotrys elegans TaxID=80388 RepID=A0A8K0SY76_9HYPO)

HSP 1 Score: 59.7 bits (143), Expect = 4.110e-5
Identity = 36/94 (38.30%), Postives = 52/94 (55.32%), Query Frame = 1
Query: 1252 MVNDLLENGASIDAEDTSLRGYTPLQLAARMRKTEMVQLLLLKGAGKDALDSKNRTPLYLAVLYGNVAAALSPGCRCGRRPSVHPIQNVGSAYS 1533
            ++  LL+ G  ++ E TSL   TPL LA R R+ ++V++LLL GA  D +D K RTPL LA   G   A        G   +V P ++ GS ++
Sbjct: 1099 LIQLLLDRGCKVNVE-TSLSRTTPLMLAVRERRADIVKMLLLDGADADVVDYKGRTPLSLATEIGGETAIQM----MGNLLAVEPSKDDGSLHN 1187          
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig5885.14347.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 13
Match NameE-valueIdentityDescription
A0A6H5KXK5_9PHAE3.990e-853.03Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A851Y5Z7_EOLRO1.100e-641.03ASB3 protein (Fragment) n=1 Tax=Eolophus roseicapi... [more]
UPI001E60D4681.740e-641.38ankyrin repeat domain-containing protein n=1 Tax=N... [more]
A0A5N5P4Y9_9ROSI1.770e-645.21Uncharacterized protein n=1 Tax=Salix brachista Ta... [more]
A0A401PMR3_SCYTO2.530e-647.83Uncharacterized protein n=1 Tax=Scyliorhinus toraz... [more]
A0A6H5JHY9_9PHAE1.130e-555.93Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7FQ25_ECTSI1.570e-552.46Similar to ankyrin 2,3/unc44 n=1 Tax=Ectocarpus si... [more]
A0A6H5JMJ6_9PHAE1.690e-565.99Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
V3ZWH9_LOTGI3.020e-536.84Uncharacterized protein n=1 Tax=Lottia gigantea Ta... [more]
A0A8K0SY76_9HYPO4.110e-538.30Putative multiple ankyrin repeats single kh domain... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig5885contigL-elsbetiae_contig5885:4682..8067 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Hectar predicted targeting categoryother localisation
Exons1
Model size3386
Cds size231
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig5885.14347.1prot_L-elsbetiae_contig5885.14347.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig5885 4781..5011 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816635.3627245-UTR-L-elsbetiae_contig5885:4681..47801622816635.3627245-UTR-L-elsbetiae_contig5885:4681..4780Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig5885 4682..4780 +
1691683701.4264557-UTR-L-elsbetiae_contig5885:4681..47801691683701.4264557-UTR-L-elsbetiae_contig5885:4681..4780Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig5885 4682..4780 +
1622816635.4120636-UTR-L-elsbetiae_contig5885:5011..80671622816635.4120636-UTR-L-elsbetiae_contig5885:5011..8067Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig5885 5012..8067 +
1691683701.4457867-UTR-L-elsbetiae_contig5885:5011..80671691683701.4457867-UTR-L-elsbetiae_contig5885:5011..8067Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig5885 5012..8067 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816635.379672-CDS-L-elsbetiae_contig5885:4780..50111622816635.379672-CDS-L-elsbetiae_contig5885:4780..5011Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5885 4781..5011 +
1691683701.4361432-CDS-L-elsbetiae_contig5885:4780..50111691683701.4361432-CDS-L-elsbetiae_contig5885:4780..5011Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5885 4781..5011 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig5885.14347.1

>prot_L-elsbetiae_contig5885.14347.1 ID=prot_L-elsbetiae_contig5885.14347.1|Name=mRNA_L-elsbetiae_contig5885.14347.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=77bp
MAAEEGKTEVVELLLLKGEEEDGLDNGGWTPLCMAVWYGHLAAALALLAA
DADVGLRCTHYENTVCSAHGGCRWAC*
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mRNA from alignment at L-elsbetiae_contig5885:4682..8067+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig5885.14347.1 ID=mRNA_L-elsbetiae_contig5885.14347.1|Name=mRNA_L-elsbetiae_contig5885.14347.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=3386bp|location=Sequence derived from alignment at L-elsbetiae_contig5885:4682..8067+ (Laminarionema elsbetiae ELsaHSoW15)
GAATCCACTCGAGGCAGCCATGGAGATGTGGTGAACGATCTGCTGGAGAA TGAAGCCTCTGTCGCCTCCAAGGATACGAATGGGAGTACTCCTCTCCACA TGGCTGCTGAAGAGGGGAAAACGGAGGTGGTAGAGCTGTTGTTGCTCAAG GGGGAGGAGGAAGACGGGCTGGACAACGGTGGTTGGACGCCTCTTTGTAT GGCCGTCTGGTATGGTCACTTGGCTGCAGCACTAGCTCTCCTGGCTGCCG ACGCCGACGTCGGCCTTCGGTGCACCCATTACGAGAACACGGTATGTAGT GCACATGGCGGCTGCAGGTGGGCATGTTGATTGACATCAAAGGGGCAGCG ATCGAGCGCGGCGCAGATGTGCACGCTGTTGACACGGCCCAAGTAACACC CCTACTTTATGCCGCATGGCACAACAAGGTGGAGGCGATCAATGTGCTCG TGAAGGCTGGGGCCAGCATCGAAGCAGGGGGTGATGACGGCTATACACCT CTTCACTATGCTACCGTTTATACCAGTCTCGAAGCCATGTTGGCCCTCTT GAAGCATGGTGCGGACATCAACGCCCGAGATCAGCGCCAACGTACGGCAT TGCACCGGGCCGCAGCCAATGCCGGGAAGCAAGAAGCCGCGGAGGCGGTG GATCTGTTGCTGAGATCAGGTGCGGACGAGACCATCCTCAACGACGACGG CGAAGCAGCCGCGGATGTTGTGGCGGAATATGTCGAAGAAAGCGACCAAG TGACCGAGGATATCGAGCGCGTGCATGAGCTGTTGGCGACGCTCCGGCCG ATAGAGCATGGCGTCACCGGGGCCATGTAGTGCTGTGCCGCGCTCATCCC GACAGAATGCGGCAGATGCAGGACAGCAGCAGCGGGCAGGCCTACACACG TTGCTACGCCAACTACGGTAACGGCGGGAGGGGAGGAACAGTAGAGTTCT ACAGCAACAGTCTGCAGCCTACTGGTTGTTGTCTACCCCGCCAAGGAAGC AAGAGGCAGCAGCATGGCAGATCCTATGGAAAGCAGAGATGTGCGAGAAG CACTTCGAAAAGCAAATGAGGAGGAGACTTTTGGACCTCATTGCCCGAGT CTTGACACCAGAAAAATGTGCCCAGTTGCTGAGGGCTCCACTCGAGCGTG CTGCACGCGAGAGCAATAGAGGCCTCGCGCAAAAGCTGGTTGGGGCGGGA GCAGAGTTTGGGGAGTCACTTCATGTAGCTATTCAGAGCGGCCATGGGGA CATGGTGAACGATCTGCTGGAGAATGGAGCATCTATCGACGCCGAGGATA CATCTCTTCGTGGCTACACTCCTCTCCAACTTGCTGCCCGAATGAGGAAA ACGGAGATGGTCCAGCTGTTGTTGCTCAAGGGGGCTGGGAAAGACGCGTT GGACAGCAAAAACCGTACACCACTTTATCTTGCGGTCTTGTATGGTAACG TGGCTGCGGCACTCTCTCCTGGCTGCCGGTGCGGACGTCGGCCTTCGGTG CACCCAATACAAAACGTCGGTAGTGCATATAGCGGCTGAAGGTGGGCTTG TTGACATCATGAGAGCGGCGATCGAGCGCGGAGCGGACGTGGATGCTGTT GACACCGCGGACCAAGCCACACCCCTTTATCGTGCTACAGTTTTCAGCGA GGTGGGGGCGATCAACGTGCGCCTGGGGGCTGGGGCCAATATTGAGGCAC GAGATCGTTACGGCCGCACAGCCCTTCCTGCTGCTGCCGGCCAGGCCAAT CTTGAAGCCGTGTTGGCCCTCTTGAAGCATGGTGCGGACGTCAACACCCA AGACCAGTACCAACAAACGCCGTTGCACCGGGCCGCATTTCAAGGTGGGC AAGGGACCGCGGAAAAAAATGGTGGATTATCTTTTGAAACCAGGTGCAGA CGAGACCATCCTCAACGACGAATGCAAAGCAGCCGCGGATGTTGTGGGAG AGGATGTTGAAGAGGAAATCCAAGTGGCCGAGGATATCGAACGTATCCGC AAGCTGTTGCCGAACGCTCCGGCCGACAGAGCATAATTTTTTGGCCAACG AACTAGCTACATGTTACGGAGTTCGTGCAAGTAACTTGGGACGGTGAGGG AGGGTTAGTGTGCCTACAACAGTCCCCCTGGAACGACTTTTTCGCGGGTT GGACGTAGACCGCCGATAACTGCAGACCATTTGTTAGATAATATCGACAG ACAGCGTGCTCAGATCGCGCCTCTACTACTGTAGCGACACAGCAGTATGT AGCCTCCACTCTATTTTTGAGCTTATTTTTTGCGATTTGGCCCAAATATT ACACCATCGTCAAGTTCACGGCTTGTTCGGCGCTCGTTTATCGCGCGCTG CCGGTTTGTCTCGATCTCAGCCTACCCTCGTAGCAGTAAACCACTCCGCA GCGCTATTGTTTGAATCACACACAACAGTGTATATATATGTATATCGACG CCGCCGCATGAGCAGGTGGTTCGGTATCCGGCAGTATTCAAATCAAGCAG GCCAGTACAAGGCCGCGAGGGGTGCGGAGTTGGTGGGAGTGTTTCGTTGG GCGTGTAAGTAGGTCCACAAAGGCGGCGGACAAACGAGTTTCGCTGTAAG AGGGAAACTCTTATCAGGGAGTAGTGGTCGTGACATCATGGTTAGGAAGA GAGTGTACACAGTAGTGTACCGCCAGTAACACGAAGGCTTCAGCAGAGGC GCGGAGAAGAGGAGAAGTAACCCGCTGGTGCTAGTGCTGGACTTGATGTT TCTCGTCTCGTTAATCGTGCCGTGACACATGTATTCCATCTCTGGACAAG TTTGCTCTTTGTTTGCGCCAGAGCATGAAGCTGACTCAACGAAAGAAGTA TATTATATGCTACACCCGATCGTACTGTTTGTCGTGTATTCACCCCGCAT TTGCGGATGAGAAGACCGGCCTTTGGCTGTCACGTTTCTTGAGCTTCAAC AGCTAACTTTCAGGAGAAATACGTTCGGCATGCTCGAGCAGATGAGAATA TACCTCCCCCCGACATCAACGCTCCATGGGCCGTGAACTGAGACGGGTTC GATTGTTCCGTGTTGCTGCAGCACTTGAAGGAGCAGCTCTGGAACTACAG CAGCAAGCGGTGTGGTGGCGATCTCTGGTTCAATTAACAATTGCCGTAAT TGTTTTGCTTCGTCACGACATTTTCTTTCTGCGGGCTGTACGAAGAAGCG TAGTCTAGAAGCGCATGGATCTGCACCTTCTAAACGGAGTCCTCGGGGGG TGCTAGAGGGAAAGAGTGGTGAAACGCATTTGCCTCTGTATGGCCAACGC CGGCAAGAGTAGCAAATGACGTCCATCCTACGAGGAGGGCGAGAACGGGC TATAAGCAGGGGGGGGTGTAGGAATGTAGACTAGAG
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Coding sequence (CDS) from alignment at L-elsbetiae_contig5885:4682..8067+

>mRNA_L-elsbetiae_contig5885.14347.1 ID=mRNA_L-elsbetiae_contig5885.14347.1|Name=mRNA_L-elsbetiae_contig5885.14347.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=462bp|location=Sequence derived from alignment at L-elsbetiae_contig5885:4682..8067+ (Laminarionema elsbetiae ELsaHSoW15)
ATGGCTGCTGAAGAGGGGAAAACGGAGGTGGTAGAGCTGTTGTTGCTCAA
GGGGGAGGAGGAAGACGGGCTGGACAACGGTGGTTGGACGCCTCTTTGTA
TGGCCGTCTGGTATGGTCACTTGGCTGCAGCACTAGCTCTCCTGGCTGCC
GACGCCGACGTCGGCCTTCGGTGCACCCATTACGAGAACACGGTATGTAG
TGCACATGGCGGCTGCAGGTGGGCATGTTGAATGGCTGCTGAAGAGGGGA
AAACGGAGGTGGTAGAGCTGTTGTTGCTCAAGGGGGAGGAGGAAGACGGG
CTGGACAACGGTGGTTGGACGCCTCTTTGTATGGCCGTCTGGTATGGTCA
CTTGGCTGCAGCACTAGCTCTCCTGGCTGCCGACGCCGACGTCGGCCTTC
GGTGCACCCATTACGAGAACACGGTATGTAGTGCACATGGCGGCTGCAGG
TGGGCATGTTGA
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