mRNA_L-elsbetiae_contig16527.4424.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig16527.4424.1
Unique NamemRNA_L-elsbetiae_contig16527.4424.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
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BLAST of mRNA_L-elsbetiae_contig16527.4424.1 vs. uniprot
Match: A0A6H5JR21_9PHAE (Lipoyl-binding domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5JR21_9PHAE)

HSP 1 Score: 214 bits (545), Expect = 3.890e-66
Identity = 106/136 (77.94%), Postives = 124/136 (91.18%), Query Frame = 1 
Query:    1 VEVGKPLCAIDGDEAAVVKAKVQAQQDEEDANAPPPEVKKPSLEDAMAGAFMAGANFVPPKKQAHKPMIKFLGKRSILREQQKDAEQQEIPGRHRHSPGALDFGQIVGGAMFGRPAMSEAEMEAVDSGGAESAPEL 408
            VEVGKPLC IDGDEA+VVKA+VQAQQDEEDANAPPPE+KKPSLEDAMA AFMAGA+FV P+K++HKP+IKF+GKR++L E++   E+  +P RHRHSPGALDF QIVGGAMFGRPAMSE EMEAVDSGGAE+AP++
Sbjct:  155 VEVGKPLCTIDGDEASVVKARVQAQQDEEDANAPPPELKKPSLEDAMAEAFMAGADFVAPRKKSHKPLIKFVGKRALLTEKKDKKEKDGLPARHRHSPGALDFSQIVGGAMFGRPAMSEEEMEAVDSGGAEAAPQV 290
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BLAST of mRNA_L-elsbetiae_contig16527.4424.1 vs. uniprot
Match: A0A835ZF23_9STRA (Single hybrid motif-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZF23_9STRA)

HSP 1 Score: 73.2 bits (178), Expect = 4.460e-12
Identity = 57/151 (37.75%), Postives = 79/151 (52.32%), Query Frame = 1 
Query:    1 VEVGKPLCAIDGDEAAVVKAKVQAQQDEEDANAPPPEVKKPSLEDAMAGAFMAGANFVPPKKQAHKPMIKFLGKRSILREQQKDAE---------------QQEIPGRH--RHSPGALDFGQIVGGAMFGRPAMSEAEMEAVDSGGAESAP 402
            V+VG PLC +D  EAA  KA+            P         +DA   A ++GA  +  + + H P+IKF+GKRS  +++  +A                +  + G    R  PGALDF QIVGGA+ GRPA+S+ EM+AV+SGGA  AP
Sbjct:  136 VQVGAPLCTLD--EAAAPKAEAPXXXXXXXXXRPE--------DDATLAAHISGA-VLEEQPRRHVPLIKFVGKRSKEKQEAPEASPAXXXXXXXXXXXASKPALRGTSGVRVVPGALDFEQIVGGALVGRPALSQEEMDAVESGGASVAP 275
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BLAST of mRNA_L-elsbetiae_contig16527.4424.1 vs. uniprot
Match: A0A7S2WGR8_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2WGR8_9STRA)

HSP 1 Score: 57.8 bits (138), Expect = 1.490e-7
Identity = 37/86 (43.02%), Postives = 46/86 (53.49%), Query Frame = 1 
Query:  172 VPPKKQAHKPMIKFLGKRSILREQQKDAEQQE--------IPG-----RHRHSPGALDFGQIVGGAMFGRPAMSEAEMEAVDSGGA 390
            V P   A  P I FLGK    R +   A+  E        +P         H+PGA+D   + GGAMFGRP +SEAEMEAV++GGA
Sbjct:   29 VAPPSSARVPSICFLGKEGWARRKHGKADIVESRSSVAMPVPSSLLAEEINHTPGAVDQWTLAGGAMFGRPPISEAEMEAVETGGA 114
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BLAST of mRNA_L-elsbetiae_contig16527.4424.1 vs. uniprot
Match: A0A7S2WFW0_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2WFW0_9STRA)

HSP 1 Score: 57.8 bits (138), Expect = 8.550e-7
Identity = 51/133 (38.35%), Postives = 65/133 (48.87%), Query Frame = 1 
Query:   37 DEAAVVKAKVQAQQDEEDANAPP--PEVKKPSLEDAMAGAFMAGANFVPPKKQAHKPMIKFLGKRSILREQQKDAEQQE--------IPG-----RHRHSPGALDFGQIVGGAMFGRPAMSEAEMEAVDSGGA 390
            DE AVV A +     + D +A P  P   KP   DA   + +A      P   A  P I FLGK    R +   A+  E        +P         H+PGA+D   + GGAMFGRP +SEAEMEAV++GGA
Sbjct:   98 DETAVVGANLFKLDTDADISAAPAAPAEAKP---DATYSSSVA------PPSSARVPSICFLGKEGWARRKHGKADIVESRSSVAMPVPSSLLAEEINHTPGAVDQWTLAGGAMFGRPPISEAEMEAVETGGA 221
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BLAST of mRNA_L-elsbetiae_contig16527.4424.1 vs. uniprot
Match: A0A7S4QSX6_9STRA (Hypothetical protein n=1 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A7S4QSX6_9STRA)

HSP 1 Score: 53.5 bits (127), Expect = 2.350e-5
Identity = 45/146 (30.82%), Postives = 72/146 (49.32%), Query Frame = 1 
Query:    1 VEVGKPLCAIDGDEAAVVKAKVQAQQDEEDANAPPPEVKKPSLEDAMAGAFMAGANFVPPKKQAHKPMIKFLGKRSILREQQKDAEQQEIPG-RHRHSPGALDFGQIVGGA----MFGRPAMSEAEMEAVDSGGAESAPELRYIDG 423
            VEVG  L  ID +    V    +    E+   +P P+ +   +E+ + G++       P +     P I FLGK      ++ + E     G + + +PG  D   I+ G+    M+GRPA SEAEMEA+ +GGA  AP+++ + G
Sbjct:   64 VEVGADLYKIDTEATPTVSGSYEISPQED--TSPHPKTEATEVEEEVVGSYTVTDTPTPVRV----PSIHFLGKDGWAA-RRNNVEPPAFTGVKVKRTPGGPDI--ILDGSGLTPMYGRPAFSEAEMEALITGGASQAPKVKKLSG 200
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BLAST of mRNA_L-elsbetiae_contig16527.4424.1 vs. uniprot
Match: A0A4D9D5P5_9STRA (Lipoyl-binding domain-containing protein n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9D5P5_9STRA)

HSP 1 Score: 53.1 bits (126), Expect = 3.060e-5
Identity = 34/80 (42.50%), Postives = 47/80 (58.75%), Query Frame = 1 
Query:  190 AHK-PMIKFLGKRSILREQQKDAE---------QQEIPGRH---RHSPGALDFGQIVGGAMFGRPAMSEAEMEAVDSGGA 390
            AH+ P+IKFLGKRS++R+Q   A+            +P RH   R +PG   F ++     FG P ++E EMEAV+SGGA
Sbjct:  122 AHRTPLIKFLGKRSLMRKQDSSAQXXXXXXXARSPSLPARHEDSRSTPGVKTFIELP--PRFGHPPVTEEEMEAVESGGA 199
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BLAST of mRNA_L-elsbetiae_contig16527.4424.1 vs. uniprot
Match: A0A7S4QTR8_9STRA (Hypothetical protein n=3 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A7S4QTR8_9STRA)

HSP 1 Score: 53.5 bits (127), Expect = 3.740e-5
Identity = 45/146 (30.82%), Postives = 72/146 (49.32%), Query Frame = 1 
Query:    1 VEVGKPLCAIDGDEAAVVKAKVQAQQDEEDANAPPPEVKKPSLEDAMAGAFMAGANFVPPKKQAHKPMIKFLGKRSILREQQKDAEQQEIPG-RHRHSPGALDFGQIVGGA----MFGRPAMSEAEMEAVDSGGAESAPELRYIDG 423
            VEVG  L  ID +    V    +    E+   +P P+ +   +E+ + G++       P +     P I FLGK      ++ + E     G + + +PG  D   I+ G+    M+GRPA SEAEMEA+ +GGA  AP+++ + G
Sbjct:  125 VEVGADLYKIDTEATPTVSGSYEISPQED--TSPHPKTEATEVEEEVVGSYTVTDTPTPVRV----PSIHFLGKDGWAA-RRNNVEPPAFTGVKVKRTPGGPDI--ILDGSGLTPMYGRPAFSEAEMEALITGGASQAPKVKKLSG 261
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig16527.4424.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 7
Match NameE-valueIdentityDescription
A0A6H5JR21_9PHAE3.890e-6677.94Lipoyl-binding domain-containing protein n=2 Tax=E... [more]
A0A835ZF23_9STRA4.460e-1237.75Single hybrid motif-containing protein n=1 Tax=Tri... [more]
A0A7S2WGR8_9STRA1.490e-743.02Hypothetical protein n=1 Tax=Rhizochromulina marin... [more]
A0A7S2WFW0_9STRA8.550e-738.35Hypothetical protein n=1 Tax=Rhizochromulina marin... [more]
A0A7S4QSX6_9STRA2.350e-530.82Hypothetical protein n=1 Tax=Ditylum brightwellii ... [more]
A0A4D9D5P5_9STRA3.060e-542.50Lipoyl-binding domain-containing protein n=1 Tax=N... [more]
A0A7S4QTR8_9STRA3.740e-530.82Hypothetical protein n=3 Tax=Ditylum brightwellii ... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig16527contigL-elsbetiae_contig16527:751..3672 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score158.7
Seed ortholog evalue1.4e-36
Seed eggNOG ortholog2880.D7FWF4
Hectar predicted targeting categoryother localisation
EggNOG free text desc.dihydrolipoyllysine-residue succinyltransferase activity
EggNOG OGsCOG0508@1,KOG0559@2759
COG Functional cat.C
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Ec32 ortholog descriptionBiotin/lipoyl attachment
Ec32 orthologEc-07_007300.1
Exons4
Model size589
Cds size303
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig16527.4424.1prot_L-elsbetiae_contig16527.4424.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig16527 1890..3524 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815684.8968868-UTR-L-elsbetiae_contig16527:750..8611622815684.8968868-UTR-L-elsbetiae_contig16527:750..861Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig16527 751..861 +
1691683036.69129-UTR-L-elsbetiae_contig16527:750..8611691683036.69129-UTR-L-elsbetiae_contig16527:750..861Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig16527 751..861 +
1622815684.9118583-UTR-L-elsbetiae_contig16527:1862..18891622815684.9118583-UTR-L-elsbetiae_contig16527:1862..1889Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig16527 1863..1889 +
1691683036.7074082-UTR-L-elsbetiae_contig16527:1862..18891691683036.7074082-UTR-L-elsbetiae_contig16527:1862..1889Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig16527 1863..1889 +
1622815684.9745324-UTR-L-elsbetiae_contig16527:3524..36721622815684.9745324-UTR-L-elsbetiae_contig16527:3524..3672Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig16527 3525..3672 +
1691683036.7472744-UTR-L-elsbetiae_contig16527:3524..36721691683036.7472744-UTR-L-elsbetiae_contig16527:3524..3672Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig16527 3525..3672 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815684.9297574-CDS-L-elsbetiae_contig16527:1889..20001622815684.9297574-CDS-L-elsbetiae_contig16527:1889..2000Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig16527 1890..2000 +
1691683036.7175875-CDS-L-elsbetiae_contig16527:1889..20001691683036.7175875-CDS-L-elsbetiae_contig16527:1889..2000Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig16527 1890..2000 +
1622815684.943152-CDS-L-elsbetiae_contig16527:2835..29911622815684.943152-CDS-L-elsbetiae_contig16527:2835..2991Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig16527 2836..2991 +
1691683036.7282934-CDS-L-elsbetiae_contig16527:2835..29911691683036.7282934-CDS-L-elsbetiae_contig16527:2835..2991Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig16527 2836..2991 +
1622815684.958732-CDS-L-elsbetiae_contig16527:3488..35241622815684.958732-CDS-L-elsbetiae_contig16527:3488..3524Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig16527 3489..3524 +
1691683036.7366126-CDS-L-elsbetiae_contig16527:3488..35241691683036.7366126-CDS-L-elsbetiae_contig16527:3488..3524Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig16527 3489..3524 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig16527.4424.1

>prot_L-elsbetiae_contig16527.4424.1 ID=prot_L-elsbetiae_contig16527.4424.1|Name=mRNA_L-elsbetiae_contig16527.4424.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=101bp
MAGAFMAGANFVPPKKQAHKPMIKFLGKRSILREQQKDAEQQEIPGRHRH
SPGALDFGQIVGGAMFGRPAMSEAEMEAVDSGGAESAPELRYIDGSRWAS
*
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mRNA from alignment at L-elsbetiae_contig16527:751..3672+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig16527.4424.1 ID=mRNA_L-elsbetiae_contig16527.4424.1|Name=mRNA_L-elsbetiae_contig16527.4424.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=2922bp|location=Sequence derived from alignment at L-elsbetiae_contig16527:751..3672+ (Laminarionema elsbetiae ELsaHSoW15)
GTGGAGGTAGGGAAGCCCCTGTGCGCGATCGACGGCGACGAGGCCGCCGT
GGTCAAGGCCAAGGTTCAGGCGCAGCAGGACGAGGAGGACGCGAACGCCC
CGCCGCCAGAGGTATAACAGCGCGTGCTCCGTGCCGTTGCTTGCTTGCTT
GCTGTTTTGTCCTCTGGCGTGCTGGGGTAAGGTTGCCTTCCGCTCACTGA
ATTTCTCCAAAACACAGCTATGTAGTAGTGTACACTTGACAAAATAGCCG
GCTGCTGCTTGTCTCGCTTCCTGTCCTGCTCTGGTGTGCAGAAGGCGCCC
GCAAAAGTAAGGTATGAGTTGAGGCTCAAATCCTCATTTCGTCGCTACTG
CTCTGCTATCGAGCGTAGAAGTAAGTTTTTTTTTGTAAAGGGTTTAGCGC
AGGCCCCACGATTGAGGTGCGGTTCTTATGCACAGCCCGATTTTCTAATC
TTTCTCGCGAAAATTCGAGGCTTATTTTCTTACATGGGTTTTTCCTGCTT
GCGGGCGCCCTCTGAATTTCGGGGGCACCGCGTGGGGCATATAGATATGT
ACGTATATTGACGGTTGTGGCACTCTCACCTGCACGGTTTCGAGTGAATC
AAAAAAGAAACGATTTTCTGAGGTTTAACCGTTTTTATTTGATGAATCCT
TCGCTGTGCCTTATAGACCTCAAAATCATTCCTGGGCAAAAGGGCTCATG
ATTATGGCCATGGTGCAAAAGCCATGCTGCAGGGGTTTGAGGTGAGTCCC
TCGACTCAGTCTCGGTGTTCGTACCCCCAAAGCATCCCCGCCACGCGCCT
TTCAGTTGGCGTCCGCCGTTGTTCCCAGATGTTGGATGTATTCTATGTAA
ATATCAGCAGGTTTGCGACTTCTGTCGTCTTTGGCTGCCGATTTCATTTT
TCTCAGGCACACTTTCCTCGAGCTGCCTCCATGGTGGTGGTGGTGGTGGT
GCTGCTGCCGCTGCCGCCGCTGCTGTTGGCGTTAGCGTCGGCGGTGGCGT
CGGCGTTGGTGTGTCGCTACCGGTGCTACTGTCACTTGTTACATTTTGGT
TTATTTTTCTGTCGGTACCGCTGTGGCCATGCTGTTGTCGATGTTCCTTG
TCGTCCTCGCAGGTGAAGAAGCCGAGCCTCGAGGACGCGATGGCCGGCGC
GTTCATGGCCGGCGCCAACTTCGTGCCCCCCAAGAAGCAAGCCCACAAGC
CCATGATAAAGTTCCTCGGAAAGCGGTCCATTCTCCGGGAGCAGCAGAAG
GTGGGTGCTTCTTGAGTTAAAAAGAACTTCGCGCTCTCGCACTTCGCACA
GTGCCAAACTTTCGATACTGCTTGTGTAAGTTCCTCCGAAAGCGGTCCAT
TCTCCGGGAGCAGCAGAAGGTGGGTGCTTCTTGAGTTAAAAATAACTTCG
CGCCCTCGCACTTCGCACAGTGCCAAAATTTCGATACCGCTTGTGCATGT
GGTATCGTGCATACACAGCGTTTTAGTGTTGAGATGCATGAGTGAAAAAA
TATTGTACATGAATTCGTTTTTCCACGAACAAACCCAACCTGCTCACGAG
ATGGCGAACGCAACAGCGACCACCACCCTTACGAGCCTAAGTGCGGCTTC
TCGAGACGTTCAGGCTCGATTTCGATGTTGTGGAAATATGAGCTTAGTCG
TGGCGCTGATGGTGGTCGCAAGCTCGCGAGCCTGCCGAACCGAGCGACCC
CATGAGGTGTTTGTTGTACTACTGCTGTGTTTGGCGTGTTTCAGGGATGG
GGAGCAATACAAAGGCCCCTACCCGCCGTTTGGGCTGCAGACGCGTGCTC
TCATGTTGTTATTATTGTTGCTGTTGTTGTCGTTGTAGTTTGGGCTGCCG
GCGCTATCTTCAAAGCTGTCCTCGCGCCTCTCCTACATACTCGCCGGGTC
CCCCCCCCCCCCGCCCAAAAGCCTTGTTTCTTTCTTTCGCGCCCCAATGT
TTTCCCGCCGCGCGCGCCGCGCTACAGTAGCATGTATGTTTGTTTGCCGC
CGACACACTCTTTTTTTTTCACAAACAATCGACGGCGCAATCTCCAACCA
TTAACCGTGACCATGACCAAATGGCCGTGTGGCAGGACGCGGAGCAACAG
GAGATCCCGGGTCGCCATCGGCACAGCCCCGGAGCGCTCGACTTCGGCCA
GATCGTGGGCGGGGCCATGTTTGGGCGGCCCGCGATGAGCGAGGCCGAGA
TGGAGGCGGTCGACAGCGGCGGCGCCGAGAGCGCGCCAGAGGTGCGTGAG
TAGTCGGGGTGTTTCGCTCGTGGTGGTGACGGTGATCACGATGGTGGATT
TGGCAATGGTGCTGCTGGTGCTGCTGCTGCTGCTGCTGGTGCTGCTGCTG
TGGTAGTAGCGGTGATGCAGTCGTGGTGGGCCACACGGGGGCTAAGTAGG
AAGGCTCGAAGGACACGCAAAGCAGTGTTGGCACGAAGCATTGGTGAAGA
AGCGGAAGAGCGCTCAGACGTTTTTGTAGTCCGCCAATGAGGAACGAGAA
CGGCGCTCTTTGTCGACCGGGGTGCATTGGTCAAACGTCAAAGCAACCCC
CAACAAGTGCAAGCATCAAAGGACTACCCCTTCCCCACCGGCTCTTCCCC
GGCCCTGTGTACGATAAAGACACGAAATAATCGTGTGGACTGCTGTTGTC
GCGCGCCTTTCCGTCCGCTCTCTTTCGCGTGCGCTTCCGACTACTACCGC
TGTTGCCGCCGTCGCTGTTCTCGGTTTTGCGCCGACAGCTGCGGTACATC
GACGGCTCGAGGTGGGCTTCGTGAGAGGGGAGGAGCGGGGCGTGCTCACA
TCGCCGCCGACGTGTGTGCGGGAGAGGAGGGAGGCGAGGGGGCGAGAGAA
AGAGTGGCATATGAAGAGAGCCACAACAAATAATCGTGTGTGATGTTTTT
TTTTTTTTTTTCCCCAATGGAC
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Coding sequence (CDS) from alignment at L-elsbetiae_contig16527:751..3672+

>mRNA_L-elsbetiae_contig16527.4424.1 ID=mRNA_L-elsbetiae_contig16527.4424.1|Name=mRNA_L-elsbetiae_contig16527.4424.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=606bp|location=Sequence derived from alignment at L-elsbetiae_contig16527:751..3672+ (Laminarionema elsbetiae ELsaHSoW15)
ATGGCCGGCGCGTTCATGGCCGGCGCCAACTTCGTGCCCCCCAAGAAGCA
AGCCCACAAGCCCATGATAAAGTTCCTCGGAAAGCGGTCCATTCTCCGGG
AGCAGCAGAAGATGGCCGGCGCGTTCATGGCCGGCGCCAACTTCGTGCCC
CCCAAGAAGCAAGCCCACAAGCCCATGATAAAGTTCCTCGGAAAGCGGTC
CATTCTCCGGGAGCAGCAGAAGGACGCGGAGCAACAGGAGATCCCGGGTC
GCCATCGGCACAGCCCCGGAGCGCTCGACTTCGGCCAGATCGTGGGCGGG
GCCATGTTTGGGCGGCCCGCGATGAGCGAGGCCGAGATGGAGGCGGTCGA
CAGCGGCGGCGCCGAGAGCGCGCCAGAGGACGCGGAGCAACAGGAGATCC
CGGGTCGCCATCGGCACAGCCCCGGAGCGCTCGACTTCGGCCAGATCGTG
GGCGGGGCCATGTTTGGGCGGCCCGCGATGAGCGAGGCCGAGATGGAGGC
GGTCGACAGCGGCGGCGCCGAGAGCGCGCCAGAGCTGCGGTACATCGACG
GCTCGAGGTGGGCTTCGTGACTGCGGTACATCGACGGCTCGAGGTGGGCT
TCGTGA
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