mRNA_L-elsbetiae_contig1471.3421.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig1471.3421.1
Unique NamemRNA_L-elsbetiae_contig1471.3421.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
BLAST of mRNA_L-elsbetiae_contig1471.3421.1 vs. uniprot
Match: D8LHA4_ECTSI (Similar to canalicular multispecific organic anion transporter n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LHA4_ECTSI)

HSP 1 Score: 203 bits (516), Expect = 3.170e-57
Identity = 118/174 (67.82%), Postives = 131/174 (75.29%), Query Frame = 2
Query:   23 PTTANVELADVSTTTAGGRRVLGEEEDVVPLLLN---SEPLSTCPAEH-VHPLPNSSYTRDDQSWASKIVFSWIDPVLDKSRAGIELQLDDTLLLPAELTAAVLSERFQVAWEALVDRGQPSLALAFRALFFREFFVAGVFRLLSELATIVAPMVLRETILFVEGKATAVPNSL 532
            P+T  VE ADV  T  G     GEE+D  PLL N   + P    PA   VHPLPNSSYTRD  SWASK+VFSW+DPVL+KSR G ELQL DTLLLP  LTA VLSERF+ +WE LV  G+PSLA AFRALFFRE+FVAG FRLL+ELA I APMVLRE ILFVEGKATAVPN++
Sbjct:    8 PSTEAVE-ADVELTGVGE----GEEDDDAPLLRNFGMATPADGAPASPGVHPLPNSSYTRDSHSWASKLVFSWVDPVLEKSRGGRELQLGDTLLLPDGLTADVLSERFRHSWEGLVSGGRPSLASAFRALFFREYFVAGAFRLLTELAKIAAPMVLREVILFVEGKATAVPNNV 176          
BLAST of mRNA_L-elsbetiae_contig1471.3421.1 vs. uniprot
Match: A0A6H5JNY7_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JNY7_9PHAE)

HSP 1 Score: 70.5 bits (171), Expect = 6.990e-13
Identity = 41/79 (51.90%), Postives = 51/79 (64.56%), Query Frame = 2
Query:   26 TTANVELADVSTTTAGGRRVLGEEEDVVPLLLNSE----PLSTCPAEHVHPLPNSSYTRDDQSWASKIVFSWIDPVLDK 250
            TT  VE ADV  T  G   + GEE+D  PLL N +     +    +  VHPLP+SSYTRD  SWASK++FSW+DPVL+K
Sbjct:   10 TTEAVE-ADVELTGVGAL-LAGEEDDDAPLLRNYDMATPAVGAAASPGVHPLPSSSYTRDSHSWASKLMFSWVDPVLEK 86          
BLAST of mRNA_L-elsbetiae_contig1471.3421.1 vs. uniprot
Match: A0A6P8WSW4_DROAB (multidrug resistance-associated protein 7 n=1 Tax=Drosophila albomicans TaxID=7291 RepID=A0A6P8WSW4_DROAB)

HSP 1 Score: 60.1 bits (144), Expect = 2.510e-7
Identity = 34/111 (30.63%), Postives = 60/111 (54.05%), Query Frame = 2
Query:  182 YTRDDQSWASKIVFSWIDPVLDKSRAGIELQLDDTLLLPAELTAAVLSERFQVAWEALVDRGQPSLALAFRALFFREFFVAGVFRLLSELATIVAPMVLRETILFVEGKAT 514
            + +D+ +WAS+ +F W+ P++DK  AG   +++D   LP  L    LSER  +A          SL  A   +F  EF++ G+ RL++++++   P++L   +   E K T
Sbjct:  224 HAQDEANWASRFLFHWVQPLIDKGVAGKLRRIEDLFDLPDALNITRLSERLHLALSQ-----SKSLLRALHKVFGLEFYLIGLLRLVADVSSFAGPLLLGALLKSDESKPT 329          
BLAST of mRNA_L-elsbetiae_contig1471.3421.1 vs. uniprot
Match: A0A6P5VPK2_DROOB (multidrug resistance-associated protein 7 n=10 Tax=obscura group TaxID=32355 RepID=A0A6P5VPK2_DROOB)

HSP 1 Score: 57.8 bits (138), Expect = 1.560e-6
Identity = 34/99 (34.34%), Postives = 57/99 (57.58%), Query Frame = 2
Query:  182 YTRDDQSWASKIVFSWIDPVLDKSRAGIELQLDDTLLLPAELTAAVLSERFQVAWEALVDRGQPSLALAFRALFFREFFVAGVFRLLSELATIVAPMVL 478
            + +D+ +WAS+ +F W+ P++DK  AG   +++D   LP  L    LSER  +A    + + Q SL  A    F  EF++ GV RL+++L+    P++L
Sbjct:  223 HAQDEANWASRFLFYWVQPLIDKGVAGKLRKIEDLFDLPDALNITRLSERLHLA----LSQSQ-SLWRALHKCFGVEFYLIGVLRLVADLSGFAGPLLL 316          
BLAST of mRNA_L-elsbetiae_contig1471.3421.1 vs. uniprot
Match: A0A6H5JWW8_9PHAE (ABC protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JWW8_9PHAE)

HSP 1 Score: 57.0 bits (136), Expect = 2.830e-6
Identity = 31/41 (75.61%), Postives = 34/41 (82.93%), Query Frame = 2
Query:  410 EFFVAGVFRLLSELATIVAPMVLRETILFVEGKATAVPNSL 532
            EF VAG FRLL+ELA I APMVLRE ILFV G ATAVPN++
Sbjct:    8 EFCVAGGFRLLTELAKIAAPMVLREVILFVGGNATAVPNNI 48          
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig1471.3421.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 5
Match NameE-valueIdentityDescription
D8LHA4_ECTSI3.170e-5767.82Similar to canalicular multispecific organic anion... [more]
A0A6H5JNY7_9PHAE6.990e-1351.90Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6P8WSW4_DROAB2.510e-730.63multidrug resistance-associated protein 7 n=1 Tax=... [more]
A0A6P5VPK2_DROOB1.560e-634.34multidrug resistance-associated protein 7 n=10 Tax... [more]
A0A6H5JWW8_9PHAE2.830e-675.61ABC protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 Ta... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig1471contigL-elsbetiae_contig1471:3488..8067 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score206.1
Seed ortholog evalue1.3e-50
Seed eggNOG ortholog2880.D8LHA4
Preferred nameABCC8
KEGG koko:K05033
KEGG TC3.A.1.208
KEGG Pathwayko02010,map02010
Hectar predicted targeting categoryother localisation
EggNOG free text desc.ATPase activity, coupled to transmembrane movement of substances
EggNOG OGsCOG1132@1,KOG0054@2759
COG Functional cat.V
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko02000,ko04147
Exons3
Model size532
Cds size516
Stop0
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig1471.3421.1prot_L-elsbetiae_contig1471.3421.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig1471 3488..8051 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815591.7750406-CDS-L-elsbetiae_contig1471:3487..36111622815591.7750406-CDS-L-elsbetiae_contig1471:3487..3611Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig1471 3488..3611 -
1691682978.3995395-CDS-L-elsbetiae_contig1471:3487..36111691682978.3995395-CDS-L-elsbetiae_contig1471:3487..3611Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig1471 3488..3611 -
1622815591.7927928-CDS-L-elsbetiae_contig1471:4833..49911622815591.7927928-CDS-L-elsbetiae_contig1471:4833..4991Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig1471 4834..4991 -
1691682978.4133837-CDS-L-elsbetiae_contig1471:4833..49911691682978.4133837-CDS-L-elsbetiae_contig1471:4833..4991Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig1471 4834..4991 -
1622815591.8066685-CDS-L-elsbetiae_contig1471:7817..80511622815591.8066685-CDS-L-elsbetiae_contig1471:7817..8051Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig1471 7818..8051 -
1691682978.4221332-CDS-L-elsbetiae_contig1471:7817..80511691682978.4221332-CDS-L-elsbetiae_contig1471:7817..8051Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig1471 7818..8051 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815591.8179696-UTR-L-elsbetiae_contig1471:8051..80671622815591.8179696-UTR-L-elsbetiae_contig1471:8051..8067Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig1471 8052..8067 -
1691682978.4308953-UTR-L-elsbetiae_contig1471:8051..80671691682978.4308953-UTR-L-elsbetiae_contig1471:8051..8067Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig1471 8052..8067 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig1471.3421.1

>prot_L-elsbetiae_contig1471.3421.1 ID=prot_L-elsbetiae_contig1471.3421.1|Name=mRNA_L-elsbetiae_contig1471.3421.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=172bp
MPPTTANVELADVSTTTAGGRRVLGEEEDVVPLLLNSEPLSTCPAEHVHP
LPNSSYTRDDQSWASKIVFSWIDPVLDKSRAGIELQLDDTLLLPAELTAA
VLSERFQVAWEALVDRGQPSLALAFRALFFREFFVAGVFRLLSELATIVA
PMVLRETILFVEGKATAVPNSL
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mRNA from alignment at L-elsbetiae_contig1471:3488..8067-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig1471.3421.1 ID=mRNA_L-elsbetiae_contig1471.3421.1|Name=mRNA_L-elsbetiae_contig1471.3421.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=4580bp|location=Sequence derived from alignment at L-elsbetiae_contig1471:3488..8067- (Laminarionema elsbetiae ELsaHSoW15)
CGATAAAAGGTTACCGATGCCTCCAACGACAGCAAATGTGGAGTTGGCGG ATGTCAGTACAACAACAGCTGGTGGTAGAAGAGTACTGGGAGAAGAGGAG GACGTTGTTCCCCTGTTGTTGAACTCGGAACCATTGAGCACATGTCCCGC TGAGCATGTGCATCCGCTCCCCAATTCCTCCTATACCCGGGACGATCAAA GCTGGGCGTCGAAGATCGTCTTCTCATGGATTGATCCGGTGCTTGACAAG GTGAGCCACTCCGCTTTACCTTTCCTATTAACTTGTTGTGTGAGATGGAT GCGTGCGTGTGCACGTAAATTCAGCAATGTGCACATAGCTATCTGGCTGT CTTCATGTGCGTGCTGTTCTGCAGTTTCGCAGCGGTAGCACGAGCTTGGA GGCAAGCAAGGCGCAGGGAGAACAACGACATGCGCCCAGAGAAATTCCAA GCCGAGGTGCTTTTTTCTCGGAGAACATTTTACCTGACTTTTGAAAAAAA TTGGCGTGCCGCACGCGAGGCTACAACTTCGACTTCGCCGTTGACGAGAG AAGGGATAGCCAGTACGTTCTCTTTTCGCGATAAAACCCAAGGCAAAGCA CCGCAGGAGGAAGGTGGGGCAAAAAATAAAAACATAGAAGTTGGTGACAA TATCACGACATCGCGGAGGGTTTCTCCCCGGAATACATGTTGTTTGACCT GGCAGAGAACTGCAGGGGAGATGTTGAATCTTCTAATATTGTAGGTGTTT TTCTACTCGGCCGGCCCAATATTACGTCACTAAATGGAATACCGAAAATT CTCACGGGAGTCCCGTCTCCAATGTGGGTTAGGTTCCATTTTTGATTATC TGCTTATCTTTCAAAAACAAAAAGAAGAAAAAACCCCAGTGTTTACCTCT TCCTGTGTGACCCCGGCGGATACTTCATGCAGTACAAAGAAGGAGCCCCG CAACGTGCATATTGTACACCGAAATGTGTGGCAGACCCCGAATAAATGGG ACGTAAACACACACACACACACACACACACACACACAACTAGATATAGCA GCAGTTGAGGACATAGATAGCATCACCTCCTCCGCGACGTGCTATGTACG CGACAACGCCAATGCAGACCAAACAAGCGATTGGTGGTATTTGTTCGCTC TAGTTGTACTTTGGCAGACGAAGCATAGCAGGTTTGCAACCCTCTGGCTA AAACTCCGGCGCTCGTGTATCTATTTATGTATCTGTCTGTAGAGGGCGGC TGCTGGTACGAAACGTAACATTAGGTTGAGCCTCATTTTCCTATTTTCGG CCGAAGCACCAGCCTGCAGCAGTAGTAGCTGATTTTCTTAAAACCAAGTC TTGTGATCATTGGTCCGTGCAGCATCTTGCCTGTTCATTTCTCAAATGTC GCATCTCCAGTTCCTACATAGTCTACATTGTGCGCTGCTTCCGTCGCACT ATCCATCACGCCATGTGCATGCGTCTAAGTGTAACCCTTTACATTTTCTT GCCCATAGCGTGAGAGGCAGCGTGGTAGGGGTGGGTATATGAGGAGGAGG AAGCGTTGCTTATTTGCCACAGTCTCTCTGAATTCAACTCAGTTTCTTCT GTCTTGGAGCTGTCCTGCGCTCAATTTCTGGTGGAGATTTCTTATGTTGT CGCCTAGAATATCTAATGAGCTAACCTTTTTATTTATTTTGCTCAAAATG TTCAGCCTCCTTTCTCTCAACGGATTGTTTCTTATATGCGGGCGCCCTCT GTGTGCAGTCCTGCGTAGTGTATGCCCTGAACCTTTACCCTAACCCCCAA GCTTGGTTGGTGCGCATCGGGAAGATTATACGTAGGTCGCTGTATCCAGT GAAGCCGAGTGGCTGGAGCGCGTTTCCAAAACACGAGCGCTGGGGTCGTT GCGTGCAATCTGCTACGTAAGCAACATCACGTTTTGTCCTATTACGTACA TATGCAATAGAGCAGACACCCAATCCTAGATGCTGGGTTGGTACAGTTGG CATTCGCGAGCAGTGAGAGGGCATCGTGCCGGCAAAGCTGTGTTTGGCCA GCTGTTGCGTGGCAGGAGCGTACGACCATGAAGGCTCGCCCCGTCTGGAG CAACAGCAGAACTCTAGTGGGACGTTCATCGAGCCGCCGCTTCAATGGCA AGCAGCATCCCACCCACGGATGCAGTTCACGGAAAGACGGCAACCGCTAC TGCCATGCTGTGAATGAGTAAACCAATAAACTAATCAGATATTTTGAGAA CAGCAGCAGTGCCGCTTGACTTTCCCCTGGATATATTGAAATCTAGGGTC TCCTTGGCTAAGCCCGCCCAAAGCGAAAGCTTAAGCTTCTCGGCATCCGG GGGAGGGCGCGTAGCCGCGAGTGCTGCTACGTCTGGGGAAAACGCTCCCG GCGATGTTTCTCAAAATGTACGCTGTGTGTTGGTTGCCCCCTCCACGAGG TGAAGCATGCTGGACTGGGCGGTTCCTCCACCCCACCCAGGCGACACACC TTGGCAGCAGAGTCTAGCCTATACCATGCACAGCTGTTTACCATGTAGCT ACGGTGCGTAATGCCACCGTAGGGCTGCACAGAGTTTGCACCAAGACTTG CTGCACCAAGTCGTGCAAGGACCGTGGTTCAACCGTAGATATGTACCATA GATGACACAAGATACCACCCCCCCCCTGGACAAATTTTTACAACTCCATT TTACTCCGTAACGAAGAGGGTACCATCACTAAACGACACGTTTTCGGACA GCAATCGACTAGATCTTTCCAACCCCACTCCCTTTCGGCACTGGCGCTCT TCTTGTTGGGGAAGAATCGACCTTTGAAAAAAACTGTCTAGGGAGGTGGT ATCTTGTGTAACCTGCGGTACAGGGGCACTCCGTTTTTCGGCAGCATAAA GGTACAATATGGAACCATCGGCCGTCGTTCTCCCCACGGCTCCATGTCTC AAGGGGGGAGGCAGTAACCCTGACTACGTCTTTTGTCCAGCGGCGACGAA ACAGAGAATAACGCAAGTGACCAATTTTATAGCCCCTGCCGCCGGCGCCG CCGTTGAACACTGCGCAACACCCCAGAGCCGGGCGGGCATTGAGCTGCAG CTGGACGACACGCTGCTCCTGCCGGCGGAGCTGACGGCCGCGGTTCTCTC CGAACGGTTCCAAGTCGCGTGGGAGGCGTTGGTCGACAGGGGGCAGCCAT CGCTTGCTTTGGCGTTCCGAGCGCTGTTTTTTCGGTGAGTGTACTGTACG AAGTACGTATGTATTTGCATCAGCAGACAAAGGGAAGACAACCCTGAAAT CGTATACTCGGGTGTCCTGCGTAAGCCCTTTTGTCGATATGTTTTAGTTC CAAATATCAGCAGCAGTGTTGCGTCCTATGCTTTGTCCTTTTCTGCGCGG TCCTTGGTTTCTTTTTTAAAAATAAACAAATAATCAAATTTGTGGGCGTG TGTCGAGCCATCGTTGTTGTTGTTGCTGCTGCTGCTGTTCCTCATGAGCT GCCGAGAAGAAAAGGCCTCAGCCGACGTCGACGCCATGTTTCAGACTTGG TGCCCCTATTTTTTATTTTGTTTTTCTTCTGGTGGGCATGTAAAGAATGG CGATATACGGCATTGCAGAGGGAACGAAAGAACCCTACAGTAATTGTGGC CCGGAGTCAATAGACGGTGGTTTGTGGGCGGATGCTCTGTTTCTTGTCTG GGGGTCAATGCTGAGCAAGCAATAGCGTACTGAACAAACATCTCAGTCGT TTGTTTTGTCTTGGCACCATCTGTCTGTTTGAGTTGAGGTGTCTTTATCG CGCCGACTTAAAATCGCTTGAAACAGATTGAACGTACCTGATTTGTTTTC TTTGCTTTGCGGAACCTTCGGGTCCATCTTATGTATTTTATGTATTTTTC GGTTTTTTAGTGTTCCCTTTATTGGGGTAAGTTATCCTGTCACCACGGGA ATCGACTGCGCCAAGTGTTGGATTAGTACGGGCTCATGGAGGTACTGGAT AGAGAGATCGGAACGGTTTTTAATGTGAAGATGACAACAACAACAACAAC AACAACAACAACTGCGTGCCGCATCTGTGCACCGCCTGGGGAACGTACGG GAGTAGCTGGGGCTACTAATGCTGCTGTGCACAGCAGCAAAGAAAACAGA GTACGTGTTGCGCGACTGCGAGTGCACGAACTTGTCTTTTTGTCACGCCG CGAAACCACCTCTCGCTCGTCAGCTCCCCTTTTGTTTCGCCCAAAGCACC TTACACACACATCGAAAACAAATTGCTTTGAAAAACAAACGATGTAGGTA TCTATGTATCAAAGACGGGACTAGCCTCCCCACATGAGCGAGACGGGGGG CTCAGTGGTGAAACGTGGGTTTTGTAAATACTCCTACACAGCAAGGTGAT GTAGGTACATACATCCCCATCCCTTTTTTTTGCGACCCGAACCCCCCCCC CCGTAGCGAGTTCTTCGTGGCCGGAGTGTTTCGGCTGCTGTCGGAGCTGG CCACGATAGTGGCGCCGATGGTGCTGCGAGAGACCATCTTGTTCGTGGAG GGCAAGGCGACGGCCGTGCCTAACAGCCTC
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Coding sequence (CDS) from alignment at L-elsbetiae_contig1471:3488..8067-

>mRNA_L-elsbetiae_contig1471.3421.1 ID=mRNA_L-elsbetiae_contig1471.3421.1|Name=mRNA_L-elsbetiae_contig1471.3421.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=1032bp|location=Sequence derived from alignment at L-elsbetiae_contig1471:3488..8067- (Laminarionema elsbetiae ELsaHSoW15)
ATGCCTCCAACGACAGCAAATGTGGAGTTGGCGGATGTCAGTACAACAAC
AGCTGGTGGTAGAAGAGTACTGGGAGAAGAGGAGGACGTTGTTCCCCTGT
TGTTGAACTCGGAACCATTGAGCACATGTCCCGCTGAGCATGTGCATCCG
CTCCCCAATTCCTCCTATACCCGGGACGATCAAAGCTGGGCGTCGAAGAT
CGTCTTCTCATGGATTGATCCGGTGCTTGACAAGATGCCTCCAACGACAG
CAAATGTGGAGTTGGCGGATGTCAGTACAACAACAGCTGGTGGTAGAAGA
GTACTGGGAGAAGAGGAGGACGTTGTTCCCCTGTTGTTGAACTCGGAACC
ATTGAGCACATGTCCCGCTGAGCATGTGCATCCGCTCCCCAATTCCTCCT
ATACCCGGGACGATCAAAGCTGGGCGTCGAAGATCGTCTTCTCATGGATT
GATCCGGTGCTTGACAAGAGCCGGGCGGGCATTGAGCTGCAGCTGGACGA
CACGCTGCTCCTGCCGGCGGAGCTGACGGCCGCGGTTCTCTCCGAACGGT
TCCAAGTCGCGTGGGAGGCGTTGGTCGACAGGGGGCAGCCATCGCTTGCT
TTGGCGTTCCGAGCGCTGTTTTTTCGAGCCGGGCGGGCATTGAGCTGCAG
CTGGACGACACGCTGCTCCTGCCGGCGGAGCTGACGGCCGCGGTTCTCTC
CGAACGGTTCCAAGTCGCGTGGGAGGCGTTGGTCGACAGGGGGCAGCCAT
CGCTTGCTTTGGCGTTCCGAGCGCTGTTTTTTCGCGAGTTCTTCGTGGCC
GGAGTGTTTCGGCTGCTGTCGGAGCTGGCCACGATAGTGGCGCCGATGGT
GCTGCGAGAGACCATCTTGTTCGTGGAGGGCAAGGCGACGGCCGTGCCTA
ACAGCCTCCGAGTTCTTCGTGGCCGGAGTGTTTCGGCTGCTGTCGGAGCT
GGCCACGATAGTGGCGCCGATGGTGCTGCGAGAGACCATCTTGTTCGTGG
AGGGCAAGGCGACGGCCGTGCCTAACAGCCTC
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