mRNA_H-elongata_contig141853.2897.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig141853.2897.1
Unique NamemRNA_H-elongata_contig141853.2897.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig141853.2897.1 vs. uniprot
Match: D7FW04_ECTSI (Magnesium chelatase n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FW04_ECTSI)

HSP 1 Score: 228 bits (580), Expect = 1.460e-66
Identity = 107/125 (85.60%), Postives = 115/125 (92.00%), Query Frame = 1 
Query:    1 TVFSFPPDKGNVGTAAYLDVFGSIHTVLLKLRKEGYEVGDFPETPRELMESILNDPEAKIESPEMNVAYRMSTQEYEELTPYAGDLVENWGQAPGHLNSDGQNLLVFGVNFGNIFIGVQPTFGYE 375
            T+FSFPPDKGNVGTAAYLDVFGSIHTVL KL+KEGY+VGD P+  R LME ILNDPEA+I+SPEMNV YRMS QEYEELTPYAGDLVENWG+APGHLNSDGQNLLVFG  FGN+FIGVQPTFGYE
Sbjct:  546 TIFSFPPDKGNVGTAAYLDVFGSIHTVLSKLKKEGYDVGDLPDDSRALMERILNDPEARIDSPEMNVEYRMSVQEYEELTPYAGDLVENWGKAPGHLNSDGQNLLVFGCKFGNVFIGVQPTFGYE 670
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BLAST of mRNA_H-elongata_contig141853.2897.1 vs. uniprot
Match: A0A7S2K5K2_9STRA (Hypothetical protein (Fragment) n=1 Tax=Leptocylindrus danicus TaxID=163516 RepID=A0A7S2K5K2_9STRA)

HSP 1 Score: 201 bits (510), Expect = 2.860e-62
Identity = 93/125 (74.40%), Postives = 109/125 (87.20%), Query Frame = 1 
Query:    1 TVFSFPPDKGNVGTAAYLDVFGSIHTVLLKLRKEGYEVGDFPETPRELMESILNDPEAKIESPEMNVAYRMSTQEYEELTPYAGDLVENWGQAPGHLNSDGQNLLVFGVNFGNIFIGVQPTFGYE 375
            T+FSFPPDKGNVGTAAYLDVF SI  VL +++KEGY++GD P +  E+MES+LNDPEAK+ SPE+NVAYRMST EY ELTPYA DL ENWG APG+LNSDGQNL+V+G  FGN+FIGVQP+FGYE
Sbjct:   43 TIFSFPPDKGNVGTAAYLDVFDSILAVLKQMKKEGYDIGDAPMSKEEIMESVLNDPEAKVSSPELNVAYRMSTDEYYELTPYATDLEENWGPAPGNLNSDGQNLVVYGKQFGNVFIGVQPSFGYE 167
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BLAST of mRNA_H-elongata_contig141853.2897.1 vs. uniprot
Match: A0A835YVC9_9STRA (Magnesium chelatase n=2 Tax=Tribonema minus TaxID=303371 RepID=A0A835YVC9_9STRA)

HSP 1 Score: 214 bits (546), Expect = 5.560e-62
Identity = 99/125 (79.20%), Postives = 114/125 (91.20%), Query Frame = 1 
Query:    1 TVFSFPPDKGNVGTAAYLDVFGSIHTVLLKLRKEGYEVGDFPETPRELMESILNDPEAKIESPEMNVAYRMSTQEYEELTPYAGDLVENWGQAPGHLNSDGQNLLVFGVNFGNIFIGVQPTFGYE 375
            T+FSFPPDKGNVGTAAYLDVFGSIH+V+ +L+ +GY+VGD PET  ELM+SILND EA+I+SPEMNVAY+MS +EYEELTPYA DL ENWG+APGHLNSDGQNLL+FG  FGN+FIGVQPTFGYE
Sbjct:  537 TIFSFPPDKGNVGTAAYLDVFGSIHSVMRELKAKGYDVGDVPETSEELMDSILNDKEARIDSPEMNVAYKMSVREYEELTPYAADLEENWGKAPGHLNSDGQNLLIFGRKFGNVFIGVQPTFGYE 661
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BLAST of mRNA_H-elongata_contig141853.2897.1 vs. uniprot
Match: A0A1Z5JTK7_FISSO (Magnesium chelatase n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5JTK7_FISSO)

HSP 1 Score: 201 bits (512), Expect = 2.110e-57
Identity = 92/125 (73.60%), Postives = 110/125 (88.00%), Query Frame = 1 
Query:    1 TVFSFPPDKGNVGTAAYLDVFGSIHTVLLKLRKEGYEVGDFPETPRELMESILNDPEAKIESPEMNVAYRMSTQEYEELTPYAGDLVENWGQAPGHLNSDGQNLLVFGVNFGNIFIGVQPTFGYE 375
            T+FSFPPDKGNVGTAAYLDVF SI  VL +L+K+GY++GD P+T  ++ME++LNDPEA+I SPE+NVAYRMST EY ELTPYA DL ENWG APG+LNSDGQNL+V+G  FGN+FIGVQP+FGYE
Sbjct:  516 TIFSFPPDKGNVGTAAYLDVFDSIKAVLAELKKQGYDIGDAPDTKEQIMEAVLNDPEARISSPELNVAYRMSTDEYYELTPYAKDLEENWGPAPGNLNSDGQNLVVYGKQFGNVFIGVQPSFGYE 640
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BLAST of mRNA_H-elongata_contig141853.2897.1 vs. uniprot
Match: A0A448ZEK5_9STRA (Magnesium chelatase n=2 Tax=Pseudo-nitzschia TaxID=41953 RepID=A0A448ZEK5_9STRA)

HSP 1 Score: 201 bits (511), Expect = 2.870e-57
Identity = 93/125 (74.40%), Postives = 109/125 (87.20%), Query Frame = 1 
Query:    1 TVFSFPPDKGNVGTAAYLDVFGSIHTVLLKLRKEGYEVGDFPETPRELMESILNDPEAKIESPEMNVAYRMSTQEYEELTPYAGDLVENWGQAPGHLNSDGQNLLVFGVNFGNIFIGVQPTFGYE 375
            T+FSFPPDKGNVGTAAYLDVF SI  VL +L+ EGY+VGD PET  E+ME++LNDPEA+I SPE+NV YRMST+EY +LTPYA DL ENWG APG+LNSDGQNL+V+G  FGN+FIGVQP+FGYE
Sbjct:  526 TIFSFPPDKGNVGTAAYLDVFDSIQAVLKQLKSEGYDVGDAPETKEEIMEAVLNDPEARISSPELNVEYRMSTEEYYDLTPYATDLEENWGPAPGNLNSDGQNLVVYGKKFGNVFIGVQPSFGYE 650
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BLAST of mRNA_H-elongata_contig141853.2897.1 vs. uniprot
Match: A0A7S0F6H0_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Phaeocystis antarctica TaxID=33657 RepID=A0A7S0F6H0_9EUKA)

HSP 1 Score: 189 bits (479), Expect = 3.470e-57
Identity = 89/125 (71.20%), Postives = 106/125 (84.80%), Query Frame = 1 
Query:    1 TVFSFPPDKGNVGTAAYLDVFGSIHTVLLKLRKEGYEVGDFPETPRELMESILNDPEAKIESPEMNVAYRMSTQEYEELTPYAGDLVENWGQAPGHLNSDGQNLLVFGVNFGNIFIGVQPTFGYE 375
            TVFSFPPDKGNVGTAAYL+VFGSI+  L  L+KEGYEVG+ PE+   L++ IL+D EA+I SPE+N+AY+M+  EY+ELTPYA DL ENWG  PG+LNSDGQNLLV+G  FGNIFIGVQP+FGYE
Sbjct:  122 TVFSFPPDKGNVGTAAYLNVFGSIYEALGNLKKEGYEVGELPESVEALVDEILHDKEARIASPELNIAYKMTVPEYKELTPYATDLEENWGPPPGNLNSDGQNLLVYGKTFGNIFIGVQPSFGYE 246
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BLAST of mRNA_H-elongata_contig141853.2897.1 vs. uniprot
Match: A0A7S3LF30_9STRA (Hypothetical protein (Fragment) n=1 Tax=Amphora coffeiformis TaxID=265554 RepID=A0A7S3LF30_9STRA)

HSP 1 Score: 191 bits (486), Expect = 6.730e-57
Identity = 91/125 (72.80%), Postives = 105/125 (84.00%), Query Frame = 1 
Query:    1 TVFSFPPDKGNVGTAAYLDVFGSIHTVLLKLRKEGYEVGDFPETPRELMESILNDPEAKIESPEMNVAYRMSTQEYEELTPYAGDLVENWGQAPGHLNSDGQNLLVFGVNFGNIFIGVQPTFGYE 375
            T+FSFPPDKGNVGTAAYLDVF SI  VL +L+ EGY++GD P     +M SIL+DPEAKI SP++NVAYRMST EY +LTPYA DL ENWG APG+LNSDGQNLLV+G  FGN+FIGVQP+FGYE
Sbjct:  114 TIFSFPPDKGNVGTAAYLDVFDSIKAVLGQLKSEGYDIGDAPMDKEAIMGSILDDPEAKISSPDLNVAYRMSTSEYYDLTPYATDLEENWGPAPGNLNSDGQNLLVYGKQFGNVFIGVQPSFGYE 238
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BLAST of mRNA_H-elongata_contig141853.2897.1 vs. uniprot
Match: A0A1C0V430_9CYAN (Magnesium chelatase n=5 Tax=Limnothrix TaxID=132605 RepID=A0A1C0V430_9CYAN)

HSP 1 Score: 198 bits (504), Expect = 2.510e-56
Identity = 95/132 (71.97%), Postives = 111/132 (84.09%), Query Frame = 1 
Query:    1 TVFSFPPDKGNVGTAAYLDVFGSIHTVLLKLRKEGYEVGDFPETPRELMESILNDPEAKIESPEMNVAYRMSTQEYEELTPYAGDLVENWGQAPGHLNSDGQNLLVFGVNFGNIFIGVQPTFGYEVRADFLL 396
            TVFSFPPDKGNVGTAAYLDVFGSIH VL  L++EGY+V D PE+P+ELME++L D +A+  SPE+NVAYRMS  EYE+LTPY+  L ENWG+ PG+LNSDGQNLLVFG +FGN+FIGVQPTFGYE     LL
Sbjct:  463 TVFSFPPDKGNVGTAAYLDVFGSIHEVLQGLKREGYDVQDVPESPKELMEAVLKDAQAQYASPELNVAYRMSVPEYEKLTPYSKKLEENWGKPPGNLNSDGQNLLVFGKHFGNVFIGVQPTFGYEGDPMLLL 594
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BLAST of mRNA_H-elongata_contig141853.2897.1 vs. uniprot
Match: A0A1E7FIS1_9STRA (Magnesium chelatase n=1 Tax=Fragilariopsis cylindrus CCMP1102 TaxID=635003 RepID=A0A1E7FIS1_9STRA)

HSP 1 Score: 197 bits (502), Expect = 4.680e-56
Identity = 91/125 (72.80%), Postives = 107/125 (85.60%), Query Frame = 1 
Query:    1 TVFSFPPDKGNVGTAAYLDVFGSIHTVLLKLRKEGYEVGDFPETPRELMESILNDPEAKIESPEMNVAYRMSTQEYEELTPYAGDLVENWGQAPGHLNSDGQNLLVFGVNFGNIFIGVQPTFGYE 375
            T+FSFPPDKGNVGTAAYLDVF SI  VL +L+ EGY++GD P T  E+ME+ILNDPEAKI SPE+N+ Y+MST+EY  LTPYA DL ENWG APG+LNSDGQNL+V+G  FGN+FIGVQP+FGYE
Sbjct:  525 TIFSFPPDKGNVGTAAYLDVFNSIQAVLGQLKSEGYDIGDAPNTKEEIMEAILNDPEAKINSPELNIEYKMSTEEYYNLTPYAKDLEENWGPAPGNLNSDGQNLVVYGKKFGNVFIGVQPSFGYE 649
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BLAST of mRNA_H-elongata_contig141853.2897.1 vs. uniprot
Match: K0T806_THAOC (Magnesium chelatase n=5 Tax=Coscinodiscophyceae TaxID=33836 RepID=K0T806_THAOC)

HSP 1 Score: 197 bits (501), Expect = 6.380e-56
Identity = 92/125 (73.60%), Postives = 108/125 (86.40%), Query Frame = 1 
Query:    1 TVFSFPPDKGNVGTAAYLDVFGSIHTVLLKLRKEGYEVGDFPETPRELMESILNDPEAKIESPEMNVAYRMSTQEYEELTPYAGDLVENWGQAPGHLNSDGQNLLVFGVNFGNIFIGVQPTFGYE 375
            T+FSFPPDKGNVGTAAYLDVF SI  VL +L+ EGY+VGD P++   +MES+LNDPEA+I SPE+NVAYRM+T EY ELTPYA DL ENWG APG+LNSDGQNL+V+G  FGN+FIGVQP+FGYE
Sbjct:  529 TIFSFPPDKGNVGTAAYLDVFDSIKAVLKQLKSEGYDVGDAPDSKELIMESVLNDPEARINSPELNVAYRMNTDEYYELTPYAKDLEENWGPAPGNLNSDGQNLVVYGKQFGNVFIGVQPSFGYE 653
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig141853.2897.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FW04_ECTSI1.460e-6685.60Magnesium chelatase n=2 Tax=Ectocarpus TaxID=2879 ... [more]
A0A7S2K5K2_9STRA2.860e-6274.40Hypothetical protein (Fragment) n=1 Tax=Leptocylin... [more]
A0A835YVC9_9STRA5.560e-6279.20Magnesium chelatase n=2 Tax=Tribonema minus TaxID=... [more]
A0A1Z5JTK7_FISSO2.110e-5773.60Magnesium chelatase n=2 Tax=Fistulifera solaris Ta... [more]
A0A448ZEK5_9STRA2.870e-5774.40Magnesium chelatase n=2 Tax=Pseudo-nitzschia TaxID... [more]
A0A7S0F6H0_9EUKA3.470e-5771.20Hypothetical protein (Fragment) n=1 Tax=Phaeocysti... [more]
A0A7S3LF30_9STRA6.730e-5772.80Hypothetical protein (Fragment) n=1 Tax=Amphora co... [more]
A0A1C0V430_9CYAN2.510e-5671.97Magnesium chelatase n=5 Tax=Limnothrix TaxID=13260... [more]
A0A1E7FIS1_9STRA4.680e-5672.80Magnesium chelatase n=1 Tax=Fragilariopsis cylindr... [more]
K0T806_THAOC6.380e-5673.60Magnesium chelatase n=5 Tax=Coscinodiscophyceae Ta... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig141853contigH-elongata_contig141853:172..1398 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score229.2
Seed ortholog evalue1.2e-57
Seed eggNOG ortholog2880.D7FW04
Preferred nameCHLH
KEGG rclassRC01012
KEGG koko:K03403
KEGG ReactionR03877
KEGG Pathwayko00860,ko01100,ko01110,map00860,map01100,map01110
GOsGO:0003674,GO:0003824,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0009507,GO:0009526,GO:0009528,GO:0009532,GO:0009536,GO:0009570,GO:0009706,GO:0009941,GO:0010007,GO:0016020,GO:0016851,GO:0016874,GO:0019866,GO:0031090,GO:0031967,GO:0031969,GO:0031975,GO:0032991,GO:0042170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044422,GO:0044424,GO:0044434,GO:0044435,GO:0044444,GO:0044446,GO:0044464,GO:0051002,GO:0051003,GO:1902494
EggNOG free text desc.ligase activity, forming nitrogen-metal bonds
EggNOG OGs2QT3B@2759,COG1429@1
EC6.6.1.1
COG Functional cat.H
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionMagnesium chelatase subunit H, putative chloroplast precursor
Ec32 orthologEc-01_002840.1
Exons3
Model size405
Cds size405
Stop1
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig141853.2897.1prot_H-elongata_contig141853.2897.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig141853 172..1398 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929842.8663168-CDS-H-elongata_contig141853:171..2731622929842.8663168-CDS-H-elongata_contig141853:171..273Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig141853 172..273 +
1691679179.7807913-CDS-H-elongata_contig141853:171..2731691679179.7807913-CDS-H-elongata_contig141853:171..273Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig141853 172..273 +
1622929842.8891277-CDS-H-elongata_contig141853:615..6891622929842.8891277-CDS-H-elongata_contig141853:615..689Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig141853 616..689 +
1691679179.793868-CDS-H-elongata_contig141853:615..6891691679179.793868-CDS-H-elongata_contig141853:615..689Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig141853 616..689 +
1622929842.9040222-CDS-H-elongata_contig141853:1169..13981622929842.9040222-CDS-H-elongata_contig141853:1169..1398Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig141853 1170..1398 +
1691679179.804929-CDS-H-elongata_contig141853:1169..13981691679179.804929-CDS-H-elongata_contig141853:1169..1398Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig141853 1170..1398 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig141853.2897.1

>prot_H-elongata_contig141853.2897.1 ID=prot_H-elongata_contig141853.2897.1|Name=mRNA_H-elongata_contig141853.2897.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=135bp
TVFSFPPDKGNVGTAAYLDVFGSIHTVLLKLRKEGYEVGDFPETPRELME
SILNDPEAKIESPEMNVAYRMSTQEYEELTPYAGDLVENWGQAPGHLNSD
GQNLLVFGVNFGNIFIGVQPTFGYEVRADFLLAG*
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mRNA from alignment at H-elongata_contig141853:172..1398+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig141853.2897.1 ID=mRNA_H-elongata_contig141853.2897.1|Name=mRNA_H-elongata_contig141853.2897.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=1227bp|location=Sequence derived from alignment at H-elongata_contig141853:172..1398+ (Himanthalia elongata Himel1 dioecious)
ACCGTCTTCAGCTTCCCCCCAGACAAGGGTAACGTAGGCACGGCCGCCTA
CCTCGACGTATTCGGATCCATCCACACCGTGCTCCTCAAGCTCCGAAAAG
AGGTACGGTTAAGATACGGATGACGAGCAACGCTATTTGAGAGTGGATGT
CCCTTTCCCCTGACCCGTCTTGATGACCTCTCGCACTCACGGAAGTATGT
TTGTTGTGAAGGCGTTTTTGTCTCGGGGTGTGGGTGTGCTTGTGTGCGTA
TTTTTTGTTTCTTCTTTTCTTCGTATTACGATTTCAGTATGTAGAGCTCC
GTGGTGCCGATGCCGCCCCATCCCCATTTTTTTATCGAACGCGTTGGCTT
TTACACATTGGGTGTTTTCTTTATGGTTTTAGCTACGCCTCCTTTATTAA
AAAAACACTTCACTCGTATCACTATTCGCTCGCTTGTTCTTAAGGGATAC
GAGGTGGGAGACTTCCCGGAGACGCCGCGGGAGCTTATGGAGAGCATCCT
AAACGATCCTGAGGCCAAGTGAGATCACCGAACCATCCTCAACCCATTAC
ACCATCTCGAAACACCCTCGCCGTCAAAAGGGGGTTCCCGCGGCATATCT
CTTTGCAAAAGCGTAGTAAAAAAGTGTATATAGAGTGTAATGTATACCGC
TGTCTCGAGGTGAAAACTAAAAATCAAAGGCGGCACGGCACTGCACAGCA
CTGCACGTACATCCGAATGTATTCTTGTAAGTGAGGTGTGTGATCGATAG
CGGCGGCTGTGAATCCCTTTCGATCAATCGTGTGTTGGTTCGTTTTTGAA
CGCGGCGCAAAAAACGGGATTGACCGACCGCTCGTCTGATTGTTCCCCCC
CTCTCCCCCGCCGCCGCGCCAACCCTTATGCTCGTGTCCCCAGATATTAA
GCCACGACCCACATACGCCCAGTTGCTTCAAGCGTGCTTCTGTTATAACC
CGTCCAGCTTTTCGTGTGAAACATCCCTTCATATCCTGTACAAAACAGGA
TCGAATCTCCGGAGATGAACGTGGCGTACCGCATGTCTACCCAGGAGTAC
GAAGAGCTCACTCCGTACGCGGGTGACCTCGTGGAGAACTGGGGCCAGGC
CCCCGGGCACCTGAATTCGGACGGGCAGAACCTCCTGGTGTTCGGGGTAA
ACTTCGGGAACATTTTCATCGGCGTACAGCCCACCTTTGGTTACGAGGTA
CGGGCTGATTTCCTGCTTGCCGGGTGA
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Coding sequence (CDS) from alignment at H-elongata_contig141853:172..1398+

>mRNA_H-elongata_contig141853.2897.1 ID=mRNA_H-elongata_contig141853.2897.1|Name=mRNA_H-elongata_contig141853.2897.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=810bp|location=Sequence derived from alignment at H-elongata_contig141853:172..1398+ (Himanthalia elongata Himel1 dioecious)
ACCGTCTTCAGCTTCCCCCCAGACAAGGGTAACGTAGGCACGGCCGCCTA
CCTCGACGTATTCGGATCCATCCACACCGTGCTCCTCAAGCTCCGAAAAG
AGACCGTCTTCAGCTTCCCCCCAGACAAGGGTAACGTAGGCACGGCCGCC
TACCTCGACGTATTCGGATCCATCCACACCGTGCTCCTCAAGCTCCGAAA
AGAGGGATACGAGGTGGGAGACTTCCCGGAGACGCCGCGGGAGCTTATGG
AGAGCATCCTAAACGATCCTGAGGCCAAGGATACGAGGTGGGAGACTTCC
CGGAGACGCCGCGGGAGCTTATGGAGAGCATCCTAAACGATCCTGAGGCC
AAGATCGAATCTCCGGAGATGAACGTGGCGTACCGCATGTCTACCCAGGA
GTACGAAGAGCTCACTCCGTACGCGGGTGACCTCGTGGAGAACTGGGGCC
AGGCCCCCGGGCACCTGAATTCGGACGGGCAGAACCTCCTGGTGTTCGGG
GTAAACTTCGGGAACATTTTCATCGGCGTACAGCCCACCTTTGGTTACGA
GGTACGGGCTGATTTCCTGCTTGCCGGGTGAGATCGAATCTCCGGAGATG
AACGTGGCGTACCGCATGTCTACCCAGGAGTACGAAGAGCTCACTCCGTA
CGCGGGTGACCTCGTGGAGAACTGGGGCCAGGCCCCCGGGCACCTGAATT
CGGACGGGCAGAACCTCCTGGTGTTCGGGGTAAACTTCGGGAACATTTTC
ATCGGCGTACAGCCCACCTTTGGTTACGAGGTACGGGCTGATTTCCTGCT
TGCCGGGTGA
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