mRNA_H-elongata_contig884.16366.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig884.16366.1
Unique NamemRNA_H-elongata_contig884.16366.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig884.16366.1 vs. uniprot
Match: D7FN49_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FN49_ECTSI)

HSP 1 Score: 157 bits (398), Expect = 2.050e-43
Identity = 78/141 (55.32%), Postives = 97/141 (68.79%), Query Frame = 2 
Query:    5 LSPEQFSRRMTEELGLSRSFILPVARSISRQLLDFRSSMPAWTSEGTVAAAEENLQMIDIDVRFRSIIYRDRLQWDVNCLNNSPESFARCTVTDLGLPQEMEAPIAFSVHQQLCKHRMAGSRSPTEEDDLIDKSRLGPHIS 427
            LSP +FSRR   ELG+  SF+ P+A+SI +QL+++    P W  EGT+A A EN+Q + IDVRFRS+I+RDRLQWDVNC  NSPE FAR TV DL LPQEME  IA + HQQ+ + RMA   S T  +D   K   GP +S
Sbjct:   39 LSPLEFSRRTCAELGIGVSFVQPIAQSIGQQLIEYGRKNPMWAVEGTIAPAGENIQTVIIDVRFRSVIFRDRLQWDVNCPYNSPEQFARITVADLNLPQEMEPIIALTTHQQVSRFRMAAPSSTTNNEDAQFKDVEGPCVS 179
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BLAST of mRNA_H-elongata_contig884.16366.1 vs. uniprot
Match: A0A507CBB2_9FUNG (Uncharacterized protein n=1 Tax=Synchytrium microbalum TaxID=1806994 RepID=A0A507CBB2_9FUNG)

HSP 1 Score: 63.9 bits (154), Expect = 1.880e-7
Identity = 56/238 (23.53%), Postives = 105/238 (44.12%), Query Frame = 2 
Query:    8 SPEQFSRRMTEELGLS--RSFILPVARSISRQLLDFRSSMPAWTSEGTVAAAEENLQMIDIDVRFRSIIYRDRLQWDVNCLNNSPESFARCTVTDLGLPQEMEAPIAFSVHQQLCKHRMAGSRSPTEEDD-----LIDKSRLGPHISFVHPVDQYEFNSRDWSNHRPTSKSTN*L*RCEQPTEEGRHELRSARPGRL*QWLNLDSQQSPHALLGVSPGSESALVLQHGW*FHNICGIS 700
            +P +F+  + E+L +     + L +A+ I  QL ++     A+ S+  +   E+    I +D+    +  +D+ +WD+N  +  PE FAR  V DLGL  E  + IA+S+ +QL +HR+       +  +     L D+   G     VH ++  +         R T+K      R ++  +     L +AR  R+     +D +        V+ G++    L+      N CG++
Sbjct:  526 TPYKFAEMLCEDLDIKPVSQYALQMAQMIQNQLSEYA----AFYSDVDLPPGEDVRVKIQLDLNCGKLHLQDQFEWDLNS-DYLPEDFARNLVADLGLGGEFVSMIAYSIREQLHRHRVTQLLEDADGGEPLVTILRDEREAGEWEPVVHVLNDEDMEKLTAERERVTAK------RLKKEAKFPPRSLINARSRRIAAGAGIDIRHMDQEQEAVTKGTDDTSALKC-----NHCGLT 747
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BLAST of mRNA_H-elongata_contig884.16366.1 vs. uniprot
Match: A0A388K753_CHABU (Uncharacterized protein n=1 Tax=Chara braunii TaxID=69332 RepID=A0A388K753_CHABU)

HSP 1 Score: 60.8 bits (146), Expect = 4.620e-7
Identity = 39/115 (33.91%), Postives = 56/115 (48.70%), Query Frame = 2 
Query:   11 PEQFSRRMTEELGLSRSFILPVARSISRQLLDFRSSMPAWTSEGTVAAAEENLQMIDIDVRFRSIIYRDRLQWDVNCLNNSPESFARCTVTDLGLPQEMEAPIAFSVHQQLCKHR 355
            P   SR++  E+GL  S+   +   I   L  F          G      E L  +D+ V F + IY+D++QWD+N   NSPE FA     DL L  +   PIA +V +Q+ +HR
Sbjct:   78 PPLISRQLCREMGLPVSYERLIEAQIRAGLKKFA---------GKEDFGGEALVTLDLSVEFGNEIYKDKVQWDLNESQNSPEMFAWQVCEDLRLKVDFMEPIAVTVREQIEQHR 183
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BLAST of mRNA_H-elongata_contig884.16366.1 vs. uniprot
Match: A5DTK9_LODEL (Uncharacterized protein n=1 Tax=Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239) TaxID=379508 RepID=A5DTK9_LODEL)

HSP 1 Score: 60.8 bits (146), Expect = 1.100e-6
Identity = 32/111 (28.83%), Postives = 60/111 (54.05%), Query Frame = 2 
Query:    5 LSPEQFSRRMTEELGLSRSFILPVARSISRQLLDFRSSMPAWTSEGTVAAAEENLQMIDIDVRFRSIIYRDRLQWDVNCLNNSPESFARCTVTDLGLPQEMEAPIAFSVHQ 337
            ++P  F+  +  +L L  S    +  SI++QL ++     ++ S  T+ + +    +ID+ V     +Y+DR++WD+N    +PE FA   V DLGL  E +  I+ ++H+
Sbjct:  155 ITPSDFAEILCNDLELPNSMGQQITDSITQQLEEY-----SYASNLTIQSKDPCNVIIDLSVNLNKQLYQDRIEWDLNQNQVTPEQFAEIVVADLGLSLEFKLAISHALHE 260
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BLAST of mRNA_H-elongata_contig884.16366.1 vs. uniprot
Match: A0A0V0R3Q0_PSEPJ (Lambda repressor-like, DNA-binding domain n=1 Tax=Pseudocohnilembus persalinus TaxID=266149 RepID=A0A0V0R3Q0_PSEPJ)

HSP 1 Score: 59.7 bits (143), Expect = 2.300e-6
Identity = 30/88 (34.09%), Postives = 55/88 (62.50%), Query Frame = 2 
Query:    2 QLSPEQFSRRMTEELGLSRSFILPVARSISRQLLDFRSSMPAWTSEGTVAAAEENLQMIDIDVRFRSIIYRDRLQWDVNCLNNSPESF 265
            QL+PEQF++ + EE    +     ++ SI R + +F+     +T+     ++EENL++I+I +R   I Y+D+ +WD++  NN+PE+F
Sbjct:  123 QLTPEQFAKIICEENNFPQQIESEISSSIKRAITNFKPYQ--YTN-----SSEENLRIIEIQIRINKIQYKDQFEWDISDQNNNPEAF 203
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BLAST of mRNA_H-elongata_contig884.16366.1 vs. uniprot
Match: A0A0U9HLK8_KLENI (Uncharacterized protein n=1 Tax=Klebsormidium nitens TaxID=105231 RepID=A0A0U9HLK8_KLENI)

HSP 1 Score: 56.2 bits (134), Expect = 1.170e-5
Identity = 34/111 (30.63%), Postives = 54/111 (48.65%), Query Frame = 2 
Query:   11 PEQFSRRMTEELGLSRSFILPVARSISRQLLDFRSSMPAWTSEGTVAAAEENLQMIDIDVRFRSIIYRDRLQWDVNCLNNSPESFARCTVTDLGLPQEMEAPIAFSVHQQL 343
            P + +R+M EELG+  +    +A  ++  L  +                +E+L  ++++V      Y+DR+ WD+N   NSP+ FA    TDLGLP     PI   V +QL
Sbjct:   42 PIKVARKMWEELGVPAALERVIATQLNVGLKKYHKK----------DLCKEHLVEVNLNVELEGRTYKDRVLWDLNEPQNSPDEFAFSVCTDLGLPMSFVDPIRTDVLKQL 142
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BLAST of mRNA_H-elongata_contig884.16366.1 vs. uniprot
Match: H8WWU6_CANO9 (Uncharacterized protein n=3 Tax=Candida TaxID=1535326 RepID=H8WWU6_CANO9)

HSP 1 Score: 57.4 bits (137), Expect = 1.440e-5
Identity = 31/111 (27.93%), Postives = 58/111 (52.25%), Query Frame = 2 
Query:    5 LSPEQFSRRMTEELGLSRSFILPVARSISRQLLDFRSSMPAWTSEGTVAAAEENLQMIDIDVRFRSIIYRDRLQWDVNCLNNSPESFARCTVTDLGLPQEMEAPIAFSVHQ 337
            ++P  F+  +  +L L    I  +A SI++Q+ ++     ++ S  ++        +ID+ V     +Y+DR++WD+N    +PE FA   V DLGL  E    I+ ++H+
Sbjct:  146 ITPTDFAAILCNDLELPNPMIGQIADSITQQIEEY-----SYASNLSIPNRNPCNVIIDLSVNLNKQLYQDRIEWDLNQNEITPEKFAEIVVADLGLSLEFNLAISHALHE 251
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig884.16366.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 7
Match NameE-valueIdentityDescription
D7FN49_ECTSI2.050e-4355.32Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A507CBB2_9FUNG1.880e-723.53Uncharacterized protein n=1 Tax=Synchytrium microb... [more]
A0A388K753_CHABU4.620e-733.91Uncharacterized protein n=1 Tax=Chara braunii TaxI... [more]
A5DTK9_LODEL1.100e-628.83Uncharacterized protein n=1 Tax=Lodderomyces elong... [more]
A0A0V0R3Q0_PSEPJ2.300e-634.09Lambda repressor-like, DNA-binding domain n=1 Tax=... [more]
A0A0U9HLK8_KLENI1.170e-530.63Uncharacterized protein n=1 Tax=Klebsormidium nite... [more]
H8WWU6_CANO91.440e-527.93Uncharacterized protein n=3 Tax=Candida TaxID=1535... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig884contigH-elongata_contig884:15707..18229 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score145.2
Seed ortholog evalue2.6e-32
Seed eggNOG ortholog2880.D7FN49
KEGG koko:K09530,ko:K11648,ko:K12662
KEGG Pathwayko03040,ko04141,ko04714,ko05225,map03040,map04141,map04714,map05225
KEGG ModuleM00354
GOsGO:0006139,GO:0006259,GO:0006281,GO:0006325,GO:0006333,GO:0006337,GO:0006338,GO:0006355,GO:0006357,GO:0006725,GO:0006807,GO:0006950,GO:0006974,GO:0006996,GO:0008150,GO:0008152,GO:0009889,GO:0009987,GO:0010468,GO:0010556,GO:0016043,GO:0019219,GO:0019222,GO:0022411,GO:0031323,GO:0031326,GO:0031498,GO:0032984,GO:0032986,GO:0033554,GO:0034641,GO:0034728,GO:0043044,GO:0043170,GO:0043933,GO:0044237,GO:0044238,GO:0044260,GO:0046483,GO:0050789,GO:0050794,GO:0050896,GO:0051171,GO:0051252,GO:0051276,GO:0051716,GO:0060255,GO:0065007,GO:0071704,GO:0071824,GO:0071840,GO:0080090,GO:0090304,GO:1901360,GO:1903506,GO:2000112,GO:2001141
EggNOG free text desc.chromatin disassembly
EggNOG OGsKOG1649@1,KOG1649@2759
COG Functional cat.K
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01009,ko03021,ko03036,ko03041,ko03110,ko04131
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionSNF5
Ec32 orthologEc-06_008390.1
Exons3
Model size1047
Cds size477
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig884.16366.1prot_H-elongata_contig884.16366.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig884 15738..17690 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931285.86196-UTR-H-elongata_contig884:15706..157371622931285.86196-UTR-H-elongata_contig884:15706..15737Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig884 15707..15737 +
1691679773.7346961-UTR-H-elongata_contig884:15706..157371691679773.7346961-UTR-H-elongata_contig884:15706..15737Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig884 15707..15737 +
1622931285.9157891-UTR-H-elongata_contig884:17690..182291622931285.9157891-UTR-H-elongata_contig884:17690..18229Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig884 17691..18229 +
1691679773.7832267-UTR-H-elongata_contig884:17690..182291691679773.7832267-UTR-H-elongata_contig884:17690..18229Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig884 17691..18229 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931285.8770547-CDS-H-elongata_contig884:15737..160071622931285.8770547-CDS-H-elongata_contig884:15737..16007Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig884 15738..16007 +
1691679773.7478468-CDS-H-elongata_contig884:15737..160071691679773.7478468-CDS-H-elongata_contig884:15737..16007Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig884 15738..16007 +
1622931285.889168-CDS-H-elongata_contig884:17212..173941622931285.889168-CDS-H-elongata_contig884:17212..17394Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig884 17213..17394 +
1691679773.7622504-CDS-H-elongata_contig884:17212..173941691679773.7622504-CDS-H-elongata_contig884:17212..17394Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig884 17213..17394 +
1622931285.9031925-CDS-H-elongata_contig884:17665..176901622931285.9031925-CDS-H-elongata_contig884:17665..17690Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig884 17666..17690 +
1691679773.7728841-CDS-H-elongata_contig884:17665..176901691679773.7728841-CDS-H-elongata_contig884:17665..17690Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig884 17666..17690 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig884.16366.1

>prot_H-elongata_contig884.16366.1 ID=prot_H-elongata_contig884.16366.1|Name=mRNA_H-elongata_contig884.16366.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=159bp
MTEELGLSRSFILPVARSISRQLLDFRSSMPAWTSEGTVAAAEENLQMID
IDVRFRSIIYRDRLQWDVNCLNNSPESFARCTVTDLGLPQEMEAPIAFSV
HQQLCKHRMAGSRSPTEEDDLIDKSRLGPHISFVHPVDQYEFNSRDWSNH
RPTSKSTN*
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mRNA from alignment at H-elongata_contig884:15707..18229+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig884.16366.1 ID=mRNA_H-elongata_contig884.16366.1|Name=mRNA_H-elongata_contig884.16366.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=2523bp|location=Sequence derived from alignment at H-elongata_contig884:15707..18229+ (Himanthalia elongata Himel1 dioecious)
TCAGCTATCGCCGGAGCAGTTCTCGAGGCGAATGACCGAAGAGCTGGGCT
TATCTCGAAGTTTCATTCTACCTGTGGCGCGATCAATCAGTCGGCAGCTG
TTGGACTTTCGAAGTTCCATGCCCGCGTGGACGTCGGAGGGGACAGTAGC
TGCGGCGGAGGAGAACCTCCAGATGATAGACATCGATGTTCGCTTCAGAT
CAATAATCTACAGAGATCGCCTGCAGTGGGACGTCAACTGCCTCAACAAC
AGTCCGGAAAGTTTCGCCAGATGCACAGTGACCGACCTTGGGCTTCCCCA
GGTACGTGCCCCACCCGCCGTAACCCGAAAACCTTGGCTACAAAGCGCGT
CACCAACTGGCAGACCCCTAAAGCCGTACGCACCTGAGTAGAAGATCTAG
ATTGGCACGTACAATTATTGGTCCAGGGGGTTACATTACCGGAATAACCG
GAATAAGATGCACCCGCTACTCAAGCTACCTTGAGGACTATTGGCTCAAT
GCCGGCGGACTTTCGGTAGTAAACGCCCTCGGTACTAGCAATTGCGTGAC
CTGATGAAATCAGGATTGACCCGATGGCGGTTGACGGTAGAGATAGACGC
CGTCGCGGAAAATGAGAGGATCCCGGGAGTACGCACAAGATTTAGCCTGG
GTTAAGGGAATGAGCAGGCTGACGCGGGACGGGACTGCTGAACCCGTCTC
GCGAGACCAAATTCTCAGATGCGAACGGGGACAGAGGAAAATTGAAAAAT
ATTTTTCCTGTTCAGTTGACGACGGGCAGAAGGCAACCAAATCCGGTTGG
TATTACTCTTGCTATACTGTAATGTGATGACCATACAGTATTCGTAGTAA
GTACATATGATCCCCCCCTAGGAGATCAATGTCCCCCCCTAGGAGATCAA
TGCGAGTGGCATAGAATGACTAGGATGACATAAATATACATACATATGAT
CCCCCCCTAGGAGGATCAATACGAGTGGCATAGAATGACTAGGACGACAG
GGCCCGGATTGCGCGTTTATGTGCAATTTAATAAATATACATACTATTTA
TTATATACCCATCTCATGTTAGGCTCAACTTTAAACGCGCTTATAGTATT
TTATTTTTAAAACCTTGAACTCATTCAAGCCCTCCGAACATCCACGCCTT
CTTAGTACCATGAGAGGTGCAGCCCAGGAGATCTCGAACAGCTCCCCATA
AGAGGGGAGTATTTGCAAACCGGCGTAGTGCCCCTTTAGGTCTCATGCCC
GTTTGCACGGTCACAACAGGTTTTTATCACAACATTTCACGTGCAACATG
CCTACATTTCTAAGAACTTATTTGCATCTTGTCCCCAACTGTCCAGAAAC
TACTTCACCAGGGTGCAATCTAGCCTAGGCAAGTTCTTCGACGCGTTGCG
TAACGTTTTGAACGCCTATTTATGTCTTATAGCGCCCTTGAATTCCCTCG
ACGCACACCATGCTCATTTTTGTATACATCCCCGACTCGGCATTACGAAC
TCCCAGGAGATGGAAGCACCCATCGCGTTCAGTGTGCACCAGCAATTATG
CAAGCATCGGATGGCGGGGTCAAGATCTCCCACCGAGGAGGATGATCTGA
TTGACAAGAGCCGCTTGGGTCCACATATATCATTCGTCCATCCCGTCGAC
CAGTATGAGTTCAATTCGAGAGACTGGAGTAACCACAGGTGACCTAGAGA
GCGAGCTCCTTCCTGTTGTAGTAGACATCTTAACCGCGGATGTAATTGAT
CACCAAATCACAAGCTCTACTCTTGGACATCAGAAACGCTTCCGAATCTT
CTCTCTCTCTGTTTGCCGTATGACCTCTTATTTCACTCTAAAGCACCAGT
CCAGCTTCAAATCACCCGATCTGCGTTTTCCTCTCCGCAGCCTTCTGTAC
TGATTATGTTTTTTTAACCATATGCAAAATATATGACATGTCTTTTCGCG
CCACGGCAGGCCAACATCAAAATCAACCAACTAGCTCTAGAGATGCGAAC
AGCCAACAGAGGAGGGGCGACACGAGCTTCGAAGTGCACGACCAGGAAGG
CTTTGACAATGGTTGAACTTGGATAGCCAGCAGAGTCCGCATGCACTTCT
GGGCGTGTCACCCGGAAGTGAGAGCGCACTCGTGCTCCAGCACGGCTGGT
AGTTTCATAACATATGTGGTATCTCATCACGTAGAGCTATCAAGCCCTTG
ACAATAATAGCAAGTATCGCCTCTTCAAGTAGATGAACACTGTGAAGTGT
AAATACCGCAATGCCACGCATCGTCGGAGTTGTGAGCGCTGGTGACAGCG
TGTTGGTGAAGTAATGAAGGAAATTGCGTCCTTTGGGTGGAATGGTCCCA
ACTCCAATTCCTATCCGCGTCCGATATGCAGTGTTTTGTAGGTCCTCTTA
TGATGAACGCCCTGTGTCCCGTCGAGAGATATTTTCATTTTTTATTTAAT
TTGGATAGATATTCAGTTGTTTGGCGGGTTTAAATGGAAATGGACCTACA
AGCCTACAAATTGGTTTAAATCG
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Coding sequence (CDS) from alignment at H-elongata_contig884:15707..18229+

>mRNA_H-elongata_contig884.16366.1 ID=mRNA_H-elongata_contig884.16366.1|Name=mRNA_H-elongata_contig884.16366.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=954bp|location=Sequence derived from alignment at H-elongata_contig884:15707..18229+ (Himanthalia elongata Himel1 dioecious)
ATGACCGAAGAGCTGGGCTTATCTCGAAGTTTCATTCTACCTGTGGCGCG
ATCAATCAGTCGGCAGCTGTTGGACTTTCGAAGTTCCATGCCCGCGTGGA
CGTCGGAGGGGACAGTAGCTGCGGCGGAGGAGAACCTCCAGATGATAGAC
ATCGATGTTCGCTTCAGATCAATAATCTACAGAGATCGCCTGCAGTGGGA
CGTCAACTGCCTCAACAACAGTCCGGAAAGTTTCGCCAGATGCACAGTGA
CCGACCTTGGGCTTCCCCAGATGACCGAAGAGCTGGGCTTATCTCGAAGT
TTCATTCTACCTGTGGCGCGATCAATCAGTCGGCAGCTGTTGGACTTTCG
AAGTTCCATGCCCGCGTGGACGTCGGAGGGGACAGTAGCTGCGGCGGAGG
AGAACCTCCAGATGATAGACATCGATGTTCGCTTCAGATCAATAATCTAC
AGAGATCGCCTGCAGTGGGACGTCAACTGCCTCAACAACAGTCCGGAAAG
TTTCGCCAGATGCACAGTGACCGACCTTGGGCTTCCCCAGGAGATGGAAG
CACCCATCGCGTTCAGTGTGCACCAGCAATTATGCAAGCATCGGATGGCG
GGGTCAAGATCTCCCACCGAGGAGGATGATCTGATTGACAAGAGCCGCTT
GGGTCCACATATATCATTCGTCCATCCCGTCGACCAGTATGAGTTCAATT
CGAGAGACTGGAGTAACCACAGGAGATGGAAGCACCCATCGCGTTCAGTG
TGCACCAGCAATTATGCAAGCATCGGATGGCGGGGTCAAGATCTCCCACC
GAGGAGGATGATCTGATTGACAAGAGCCGCTTGGGTCCACATATATCATT
CGTCCATCCCGTCGACCAGTATGAGTTCAATTCGAGAGACTGGAGTAACC
ACAGGCCAACATCAAAATCAACCAACTAGGCCAACATCAAAATCAACCAA
CTAG
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