mRNA_H-elongata_contig60.13533.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig60.13533.1
Unique NamemRNA_H-elongata_contig60.13533.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig60.13533.1 vs. uniprot
Match: D8LD12_ECTSI (Argininosuccinate synthase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LD12_ECTSI)

HSP 1 Score: 89.4 bits (220), Expect = 5.570e-19
Identity = 45/89 (50.56%), Postives = 58/89 (65.17%), Query Frame = 2 
Query:    2 VRFEMYVQALDSTMSVITPSTDSDFLKCFKDRADFQAYAEEHDLITSSTQTASNWVDRNIYHTSYNMGIIEGPVRRSSPEILKTTVEPV 268
            VRFEM  QALDS ++ I P  D++FL+ FK R D  AYA E  +  SST  AS  +D N+YHTSY  GI+E P+    PE+ K TV+P+
Sbjct:  138 VRFEMCAQALDSKITTIAPWRDAEFLEKFKGRGDLLAYATEQGIPVSSTPKASYSIDENLYHTSYESGILEDPMTAPPPEMFKMTVDPL 226
[Close]
BLAST of mRNA_H-elongata_contig60.13533.1 vs. uniprot
Match: A0A7R9YC46_9STRA (Argininosuccinate synthase n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9YC46_9STRA)

HSP 1 Score: 82.4 bits (202), Expect = 1.740e-16
Identity = 41/88 (46.59%), Postives = 55/88 (62.50%), Query Frame = 2 
Query:    2 VRFEMYVQALDSTMSVITPSTDSDFLKCFKDRADFQAYAEEHDLITSSTQTASNWVDRNIYHTSYNMGIIEGPVRRSSPEILKTTVEP 265
            VRFEM  QALD+ +  I P  D++FL+ FK R D  AYA EH +   +T  A   VD N+YHTSY  G++E P+   +PE+ K T +P
Sbjct:  131 VRFEMCAQALDANLVTIAPWRDAEFLEKFKGRQDLLAYAAEHGIPVDATPKAPYSVDENLYHTSYESGMLEDPMTPPTPEMFKMTRDP 218
[Close]
BLAST of mRNA_H-elongata_contig60.13533.1 vs. uniprot
Match: A0A067D2C0_SAPPC (Argininosuccinate synthase n=2 Tax=Saprolegnia TaxID=4769 RepID=A0A067D2C0_SAPPC)

HSP 1 Score: 80.9 bits (198), Expect = 5.990e-16
Identity = 38/88 (43.18%), Postives = 56/88 (63.64%), Query Frame = 2 
Query:    2 VRFEMYVQALDSTMSVITPSTDSDFLKCFKDRADFQAYAEEHDLITSSTQTASNWVDRNIYHTSYNMGIIEGPVRRSSPEILKTTVEP 265
            VRFE+  QAL++++  I P  D +F++ FK R D  AYA+ H++   +T  A   VD N+YHTSY  G++E P+    PE+ K TV+P
Sbjct:  131 VRFELCAQALNASLKTIAPWRDPEFIEAFKGRQDLLAYAKAHNIPVDATPKAPYSVDENLYHTSYESGMLEDPMTGPLPEMFKMTVDP 218
[Close]
BLAST of mRNA_H-elongata_contig60.13533.1 vs. uniprot
Match: A0A7S1UIZ0_9STRA (Argininosuccinate synthase n=1 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1UIZ0_9STRA)

HSP 1 Score: 80.9 bits (198), Expect = 6.330e-16
Identity = 41/88 (46.59%), Postives = 55/88 (62.50%), Query Frame = 2 
Query:    2 VRFEMYVQALDSTMSVITPSTDSDFLKCFKDRADFQAYAEEHDLITSSTQTASNWVDRNIYHTSYNMGIIEGPVRRSSPEILKTTVEP 265
            VRFEM  QALD+++S I P  D++FL+ FK R D   YA EH +   +T  A   VD N+YHTSY  G++E  +    PE+ K TV+P
Sbjct:  136 VRFEMCAQALDASLSTIAPWRDAEFLEKFKGRTDLLNYAAEHGIPVDATPKAPYSVDENLYHTSYESGMLEDTMVGPLPEMFKMTVDP 223
[Close]
BLAST of mRNA_H-elongata_contig60.13533.1 vs. uniprot
Match: A0A836CG55_9STRA (Argininosuccinate synthase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CG55_9STRA)

HSP 1 Score: 80.5 bits (197), Expect = 8.370e-16
Identity = 39/88 (44.32%), Postives = 56/88 (63.64%), Query Frame = 2 
Query:    2 VRFEMYVQALDSTMSVITPSTDSDFLKCFKDRADFQAYAEEHDLITSSTQTASNWVDRNIYHTSYNMGIIEGPVRRSSPEILKTTVEP 265
            VRFEM  QALD+T++ I P  + +F++ FK R+D  AYA +H++  SST  A+  +D N+YHTSY  G++E P       + K TV P
Sbjct:  134 VRFEMCAQALDATITTIAPWREPEFIERFKGRSDLLAYAAQHNIPVSSTPKANYSIDENLYHTSYESGMLEDPACAYPESMFKMTVSP 221
[Close]
BLAST of mRNA_H-elongata_contig60.13533.1 vs. uniprot
Match: A0A7S2B2G5_9STRA (Argininosuccinate synthase n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2B2G5_9STRA)

HSP 1 Score: 79.7 bits (195), Expect = 1.590e-15
Identity = 41/88 (46.59%), Postives = 55/88 (62.50%), Query Frame = 2 
Query:    2 VRFEMYVQALDSTMSVITPSTDSDFLKCFKDRADFQAYAEEHDLITSSTQTASNWVDRNIYHTSYNMGIIEGPVRRSSPEILKTTVEP 265
            VRFEM  QALD+T++ I P  D +FL  FK R D  AYA EH++   +T  A   VD NI+HTS+  G++E P+     E+ K TV+P
Sbjct:  138 VRFEMNAQALDATITTIAPWRDPEFLTRFKGRTDLLAYAAEHNIPVDATPKAPYSVDENIFHTSFESGMLEDPMVSPLKEMWKMTVDP 225
[Close]
BLAST of mRNA_H-elongata_contig60.13533.1 vs. uniprot
Match: A0A024T7I8_9STRA (Argininosuccinate synthase (Fragment) n=13 Tax=Aphanomyces TaxID=100860 RepID=A0A024T7I8_9STRA)

HSP 1 Score: 79.3 bits (194), Expect = 2.070e-15
Identity = 38/88 (43.18%), Postives = 56/88 (63.64%), Query Frame = 2 
Query:    2 VRFEMYVQALDSTMSVITPSTDSDFLKCFKDRADFQAYAEEHDLITSSTQTASNWVDRNIYHTSYNMGIIEGPVRRSSPEILKTTVEP 265
            VRFE+  QAL++ +  I P  D++F++ FK R D  AYA+E ++   +T  A   VD N+YHTSY  G++E P+    PE+ K TV+P
Sbjct:  144 VRFELCTQALNANLKTIAPWRDTEFIEKFKGRQDLIAYAKEQNIPIDATPKAPYSVDENLYHTSYESGMLEDPMTAPMPEMFKMTVDP 231
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BLAST of mRNA_H-elongata_contig60.13533.1 vs. uniprot
Match: A0A1V9YYF7_9STRA (Argininosuccinate synthase n=1 Tax=Achlya hypogyna TaxID=1202772 RepID=A0A1V9YYF7_9STRA)

HSP 1 Score: 79.0 bits (193), Expect = 2.910e-15
Identity = 38/88 (43.18%), Postives = 56/88 (63.64%), Query Frame = 2 
Query:    2 VRFEMYVQALDSTMSVITPSTDSDFLKCFKDRADFQAYAEEHDLITSSTQTASNWVDRNIYHTSYNMGIIEGPVRRSSPEILKTTVEP 265
            VRFE+  QAL++++  I P  D++F++ FK R D  AYA+E  +   +T  A   VD N+YHTSY  G++E P+    PE+ K TV+P
Sbjct:  131 VRFELCAQALNASLKTIAPWRDTEFIEKFKGRQDLIAYAQEQGIPIDATPKAPYSVDENLYHTSYESGMLEDPMTGPMPEMFKMTVDP 218
[Close]
BLAST of mRNA_H-elongata_contig60.13533.1 vs. uniprot
Match: A0A0P1ABP3_PLAHL (Argininosuccinate synthase n=1 Tax=Plasmopara halstedii TaxID=4781 RepID=A0A0P1ABP3_PLAHL)

HSP 1 Score: 78.2 bits (191), Expect = 5.140e-15
Identity = 38/88 (43.18%), Postives = 55/88 (62.50%), Query Frame = 2 
Query:    2 VRFEMYVQALDSTMSVITPSTDSDFLKCFKDRADFQAYAEEHDLITSSTQTASNWVDRNIYHTSYNMGIIEGPVRRSSPEILKTTVEP 265
            VRFE+  QALD+ +  I P  D++F++ FK RAD   YA+E ++   +T  A   VD N+YHTSY  G++E P+     E+ K TV+P
Sbjct:  131 VRFELCAQALDAQLKTIAPWRDTEFIEMFKGRADLIQYAKEQNIPIDATPKAPYSVDENLYHTSYEAGMLEDPMTAPMVEMFKMTVDP 218
[Close]
BLAST of mRNA_H-elongata_contig60.13533.1 vs. uniprot
Match: A0A024FZ42_9STRA (Argininosuccinate synthase n=2 Tax=Albugo TaxID=65356 RepID=A0A024FZ42_9STRA)

HSP 1 Score: 78.2 bits (191), Expect = 5.500e-15
Identity = 38/88 (43.18%), Postives = 54/88 (61.36%), Query Frame = 2 
Query:    2 VRFEMYVQALDSTMSVITPSTDSDFLKCFKDRADFQAYAEEHDLITSSTQTASNWVDRNIYHTSYNMGIIEGPVRRSSPEILKTTVEP 265
            VRFE+  QALD+ +  I P  D++F+  FK RAD   YA E  +   +T  AS  +D N+YHTSY  G++E P+   + E+ K TV+P
Sbjct:  131 VRFELCAQALDAKLQTIAPWRDAEFINQFKGRADLLKYAAEQKIPVDATPKASYSMDENLYHTSYEAGMLEDPLTAPTSEMFKMTVDP 218
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig60.13533.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LD12_ECTSI5.570e-1950.56Argininosuccinate synthase n=1 Tax=Ectocarpus sili... [more]
A0A7R9YC46_9STRA1.740e-1646.59Argininosuccinate synthase n=1 Tax=Pinguiococcus p... [more]
A0A067D2C0_SAPPC5.990e-1643.18Argininosuccinate synthase n=2 Tax=Saprolegnia Tax... [more]
A0A7S1UIZ0_9STRA6.330e-1646.59Argininosuccinate synthase n=1 Tax=Phaeomonas parv... [more]
A0A836CG55_9STRA8.370e-1644.32Argininosuccinate synthase n=1 Tax=Tribonema minus... [more]
A0A7S2B2G5_9STRA1.590e-1546.59Argininosuccinate synthase n=1 Tax=Dictyocha specu... [more]
A0A024T7I8_9STRA2.070e-1543.18Argininosuccinate synthase (Fragment) n=13 Tax=Aph... [more]
A0A1V9YYF7_9STRA2.910e-1543.18Argininosuccinate synthase n=1 Tax=Achlya hypogyna... [more]
A0A0P1ABP3_PLAHL5.140e-1543.18Argininosuccinate synthase n=1 Tax=Plasmopara hals... [more]
A0A024FZ42_9STRA5.500e-1543.18Argininosuccinate synthase n=2 Tax=Albugo TaxID=65... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig60contigH-elongata_contig60:28022..28982 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score82.4
Seed ortholog evalue1.1e-13
Seed eggNOG ortholog2880.D8LD12
Preferred nameASS1
KEGG rclassRC00380,RC00629
KEGG koko:K01940
KEGG ReactionR01954
KEGG Pathwayko00220,ko00250,ko01100,ko01110,ko01130,ko01230,ko05418,map00220,map00250,map01100,map01110,map01130,map01230,map05418
KEGG ModuleM00029,M00844,M00845
GOsGO:0000050,GO:0000052,GO:0000053,GO:0000323,GO:0001101,GO:0001505,GO:0001655,GO:0001822,GO:0001889,GO:0002237,GO:0002376,GO:0002526,GO:0003674,GO:0003824,GO:0004055,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005739,GO:0005740,GO:0005741,GO:0005764,GO:0005773,GO:0005783,GO:0005829,GO:0006082,GO:0006520,GO:0006525,GO:0006526,GO:0006531,GO:0006575,GO:0006807,GO:0006950,GO:0006952,GO:0006953,GO:0006954,GO:0006955,GO:0007162,GO:0007275,GO:0007494,GO:0007517,GO:0007568,GO:0007584,GO:0007623,GO:0008150,GO:0008152,GO:0008652,GO:0009058,GO:0009064,GO:0009066,GO:0009084,GO:0009410,GO:0009605,GO:0009607,GO:0009617,GO:0009636,GO:0009719,GO:0009725,GO:0009889,GO:0009891,GO:0009893,GO:0009987,GO:0009991,GO:0010033,GO:0010035,GO:0010038,GO:0010043,GO:0010046,GO:0010243,GO:0012505,GO:0014070,GO:0014074,GO:0014075,GO:0015643,GO:0016020,GO:0016053,GO:0016597,GO:0016874,GO:0016879,GO:0019222,GO:0019627,GO:0019752,GO:0019867,GO:0022407,GO:0022408,GO:0030155,GO:0031090,GO:0031323,GO:0031325,GO:0031326,GO:0031328,GO:0031406,GO:0031667,GO:0031960,GO:0031966,GO:0031967,GO:0031968,GO:0031975,GO:0032355,GO:0032496,GO:0032501,GO:0032502,GO:0032870,GO:0033554,GO:0033762,GO:0033993,GO:0034097,GO:0034201,GO:0034341,GO:0034405,GO:0034612,GO:0034616,GO:0034641,GO:0035295,GO:0035690,GO:0036094,GO:0036477,GO:0042221,GO:0042493,GO:0042802,GO:0042995,GO:0043005,GO:0043025,GO:0043167,GO:0043168,GO:0043177,GO:0043200,GO:0043204,GO:0043207,GO:0043209,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043434,GO:0043436,GO:0043603,GO:0043604,GO:0043648,GO:0044237,GO:0044238,GO:0044249,GO:0044271,GO:0044281,GO:0044283,GO:0044297,GO:0044422,GO:0044424,GO:0044429,GO:0044444,GO:0044446,GO:0044464,GO:0045087,GO:0045428,GO:0045429,GO:0046394,GO:0046683,GO:0048511,GO:0048513,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048545,GO:0048565,GO:0048731,GO:0048732,GO:0048856,GO:0050789,GO:0050794,GO:0050896,GO:0051171,GO:0051173,GO:0051384,GO:0051591,GO:0051704,GO:0051707,GO:0051716,GO:0055123,GO:0060359,GO:0060416,GO:0060538,GO:0060539,GO:0060541,GO:0061008,GO:0061061,GO:0065007,GO:0065008,GO:0070542,GO:0070852,GO:0070887,GO:0071216,GO:0071219,GO:0071222,GO:0071229,GO:0071230,GO:0071242,GO:0071310,GO:0071320,GO:0071345,GO:0071346,GO:0071356,GO:0071375,GO:0071377,GO:0071383,GO:0071384,GO:0071385,GO:0071396,GO:0071398,GO:0071400,GO:0071407,GO:0071417,GO:0071418,GO:0071466,GO:0071495,GO:0071498,GO:0071499,GO:0071548,GO:0071549,GO:0071704,GO:0071941,GO:0072001,GO:0072350,GO:0097327,GO:0097458,GO:0098588,GO:0098805,GO:0120025,GO:1901564,GO:1901566,GO:1901576,GO:1901605,GO:1901607,GO:1901652,GO:1901653,GO:1901654,GO:1901655,GO:1901698,GO:1901699,GO:1901700,GO:1901701,GO:1903037,GO:1903038,GO:1903426,GO:1903428,GO:1904407,GO:2000377,GO:2000379
EggNOG free text desc.argininosuccinate synthase activity
EggNOG OGsCOG0137@1,KOG1706@2759
Ec32 ortholog descriptionargininosuccinate synthetase
Ec32 orthologEc-01_001870.1
EC6.3.4.5
COG Functional cat.E
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko04147
Hectar predicted targeting categoryother localisation
Exons3
Model size268
Cds size255
Stop0
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig60.13533.1prot_H-elongata_contig60.13533.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig60 28022..28969 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930973.0638735-CDS-H-elongata_contig60:28021..281351622930973.0638735-CDS-H-elongata_contig60:28021..28135Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig60 28022..28135 -
1691679646.681368-CDS-H-elongata_contig60:28021..281351691679646.681368-CDS-H-elongata_contig60:28021..28135Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig60 28022..28135 -
1622930973.0881298-CDS-H-elongata_contig60:28330..283931622930973.0881298-CDS-H-elongata_contig60:28330..28393Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig60 28331..28393 -
1691679646.7011504-CDS-H-elongata_contig60:28330..283931691679646.7011504-CDS-H-elongata_contig60:28330..28393Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig60 28331..28393 -
1622930973.1071544-CDS-H-elongata_contig60:28891..289691622930973.1071544-CDS-H-elongata_contig60:28891..28969Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig60 28892..28969 -
1691679646.7149477-CDS-H-elongata_contig60:28891..289691691679646.7149477-CDS-H-elongata_contig60:28891..28969Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig60 28892..28969 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930973.1296542-UTR-H-elongata_contig60:28969..289821622930973.1296542-UTR-H-elongata_contig60:28969..28982Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig60 28970..28982 -
1691679646.7257204-UTR-H-elongata_contig60:28969..289821691679646.7257204-UTR-H-elongata_contig60:28969..28982Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig60 28970..28982 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig60.13533.1

>prot_H-elongata_contig60.13533.1 ID=prot_H-elongata_contig60.13533.1|Name=mRNA_H-elongata_contig60.13533.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=85bp
MYVQALDSTMSVITPSTDSDFLKCFKDRADFQAYAEEHDLITSSTQTASN
WVDRNIYHTSYNMGIIEGPVRRSSPEILKTTVEPV
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mRNA from alignment at H-elongata_contig60:28022..28982-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig60.13533.1 ID=mRNA_H-elongata_contig60.13533.1|Name=mRNA_H-elongata_contig60.13533.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=961bp|location=Sequence derived from alignment at H-elongata_contig60:28022..28982- (Himanthalia elongata Himel1 dioecious)
GGTTCGCTTCGAGATGTACGTTCAAGCGCTAGACTCGACGATGTCAGTCA
TAACGCCGTCGACCGATTCAGATTTCTTAAAGTGCTTTAAGGTGCGTTGT
GATTTGTAAGACACTAGCTCGAAACACCAGTCATGTGATTCCGGATGCCA
ATTGTTATAAAATACACGCCATTCTGAAAGGGAGGCTAACTTTCTTTCCC
ACTAAGACAACGTGAAAGTCGATACTTGCTTGGAAAGCGACTGGTGGCCA
CTGACATTAACAGGACGGACGTATACTCTCCATTCATAAACCGAAGCGAT
GCGTGTGTGACCTCGTCATCATTTTGTTATATAACTCCGTCCTTTAAGGG
TTTTGGTGATCGCTAGTGTGCGATACTGTTGTGCCACATAATAGTATCAA
CGCTCGAGTCTCTAGTGCACTCTTACCTTAGCCTTAGGGTCTTGGATTCT
GGCGTCTAAATTCATTGTATGTGGTATTAAGATATGTTTTTGAAGACACG
TTTCTGCTTGTCGTTTAGTATTTTGTTGTAGAAGTATATTAAAGTCTTCG
AAACACGTCTTATACCGTTGTCCAATTTCCGCGTCCCAGGATCGTGCGGA
TTTCCAGGCCTATGCCGAGGAGCATGACCTCATTACTTCCTCTACACAAA
CGGTCCGTGCAGATCTTGCGTGCAAACCATTATACATGCATCATCATGCA
GCTTGGGTCAAGAGTCCAATACGTTCTTAATTGTCTCACCCCTTTTTCTC
ACTCTCTTTTAACTTAACACTTTGGGTGATCGCGAAATATGTTACGCCGT
CTTAACATAAGACATTGACTAACATAATTAAAATACCTATCCGGCAGGCG
TCAAACTGGGTTGACCGAAACATCTATCATACCTCTTACAATATGGGCAT
AATTGAGGGCCCGGTGAGGCGTTCGTCCCCCGAGATACTCAAGACCACGG
TAGAGCCCGTC
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Coding sequence (CDS) from alignment at H-elongata_contig60:28022..28982-

>mRNA_H-elongata_contig60.13533.1 ID=mRNA_H-elongata_contig60.13533.1|Name=mRNA_H-elongata_contig60.13533.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=510bp|location=Sequence derived from alignment at H-elongata_contig60:28022..28982- (Himanthalia elongata Himel1 dioecious)
ATGTACGTTCAAGCGCTAGACTCGACGATGTCAGTCATAACGCCGTCGAC
CGATTCAGATTTCTTAAAGTGCTTTAAGATGTACGTTCAAGCGCTAGACT
CGACGATGTCAGTCATAACGCCGTCGACCGATTCAGATTTCTTAAAGTGC
TTTAAGGATCGTGCGGATTTCCAGGCCTATGCCGAGGAGCATGACCTCAT
TACTTCCTCTACACAAACGGATCGTGCGGATTTCCAGGCCTATGCCGAGG
AGCATGACCTCATTACTTCCTCTACACAAACGGCGTCAAACTGGGTTGAC
CGAAACATCTATCATACCTCTTACAATATGGGCATAATTGAGGGCCCGGT
GAGGCGTTCGTCCCCCGAGATACTCAAGACCACGGTAGAGCCCGTCGCGT
CAAACTGGGTTGACCGAAACATCTATCATACCTCTTACAATATGGGCATA
ATTGAGGGCCCGGTGAGGCGTTCGTCCCCCGAGATACTCAAGACCACGGT
AGAGCCCGTC
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