mRNA_H-elongata_contig52536.12558.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig52536.12558.1
Unique NamemRNA_H-elongata_contig52536.12558.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig52536.12558.1 vs. uniprot
Match: D8LQQ0_ECTSI (GPI ethanolamine phosphate transferase 3 n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LQQ0_ECTSI)

HSP 1 Score: 306 bits (785), Expect = 9.330e-87
Identity = 249/619 (40.23%), Postives = 292/619 (47.17%), Query Frame = 3 
Query:    3 AAAQESIAGVGLSTEAAFGIARSLLPLPALWCLCASARG-------------FGNVERGKQRNSVDVTATTAAVSAVFDYYPFGFQFHGVMQAISLAATGAYWINDVAVEAREGAATAAAAVMRENARWSAYG-----------GGLAALPPLRLFLPRAVYFLCAVGFATALLLPVGRARARSKPQLWPRLRAQGRSEGGTGDYPPQRSLSVQRGPPGEAASNAPARASSDSRSENRGDGFEFFGNTCSALGRASCGDPSVRLSYHHAAARTSAALVSHLLPVVVLLLGPGSPAIVLLVAAACGFALRGVSIAAGA-----------GVMVPLGAVALAWSVVGRAFFFLTGHHNQFSRLQYSAAFVGFDEFNFKIGGVMLFLNTFGTEILAALALPMLVAATVXXXXXXXXXXXXXXXXXXXXLQQTYQDPEEFSDKVADELRGKRRQRHXXXXXXXXXXXDAQRSFLEQPRKEVWLEASQSRRGKSGATARRGKSTPSSLGGRHPSDDGVWGAGVTTASGSRRWGDARETVPNPRSSASSAEEAFLSSMERISGLVLALSSARTLLSAANIAVQRGHLMLWAVFAPKFVFDATMQAVCGAAAVATWAVILVVHRGF 1754
            AA Q+S A  G+STEAAFG++RSLLPLPALW LC  ARG              G V+    R +    + +  V      +      HG +QA+SLAA GAYW+N+V   A                                 G L  LPP RL LPRA Y LC  G A ALL P GR R + +                  D  PQ+         GE                         G TC+  G       S   SY  + A T+A +VSHLLPVVVLLLGPGSPA+V  V+AACG  LR VS+ A             G ++PLGAVA++WSVVGRAFFFLTGHHNQFSRLQYSAAFVGFDEF+FK+GGV+LFLNTFGTEILAALALP+  AA                             P    + V+   R   RQ                 S    P                   + R  S      G HP D G   A                T             + LS+MER+SGL L LS+ RT LSAAN++VQRGHLMLWAVFAPKFVFDATMQAVCGAAAV  WAV++V HR +
Sbjct:  816 AAVQDSAAEGGVSTEAAFGVSRSLLPLPALWYLCRLARGGXXXXXDGRMLWGSGAVDVANTRGAPAAVSLSGGVLGAASGWARRRCSHGAVQALSLAAIGAYWVNEVVAAAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSL--LPPARLLLPRATYLLCLAGLAVALLRPHGRRRRKQQ------------------DPVPQK---------GEE------------------------GRTCTPPGG---NGSSSSWSYAQSLAGTAATVVSHLLPVVVLLLGPGSPAVVAFVSAACGCVLRSVSVTAAGRTTXXXXXXRGGAVLPLGAVAVSWSVVGRAFFFLTGHHNQFSRLQYSAAFVGFDEFDFKVGGVLLFLNTFGTEILAALALPLAAAAAAASGVSQDAA------------------PPVLPEAVSGSSRPNDRQ-----------------SLSSPP-------------------SPRPHSVAGRWDGNHPDDAGNDAAXXXXXXXXXXXXXXETT-------------SMLSAMERLSGLTLLLSAIRTFLSAANVSVQRGHLMLWAVFAPKFVFDATMQAVCGAAAVLAWAVVVVAHRAY 1311
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BLAST of mRNA_H-elongata_contig52536.12558.1 vs. uniprot
Match: A0A4D9CYS9_9STRA (GPI ethanolamine phosphate transferase 3 n=2 Tax=Monodopsidaceae TaxID=425072 RepID=A0A4D9CYS9_9STRA)

HSP 1 Score: 87.0 bits (214), Expect = 6.740e-14
Identity = 43/59 (72.88%), Postives = 51/59 (86.44%), Query Frame = 3 
Query:  912 LAWSVVGRAFFFLTGHHNQFSRLQYSAAFVGFDEFNFKIGGVMLFLNTFGTEILAALAL 1088
            +A S +GRAFFF TGHH+QF+RLQYS+AFVGFDEF+F IGG +LFLNTFGTE+L  L L
Sbjct: 1010 VAVSFLGRAFFFATGHHSQFNRLQYSSAFVGFDEFSFVIGGCLLFLNTFGTELLVLLLL 1068
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BLAST of mRNA_H-elongata_contig52536.12558.1 vs. uniprot
Match: A0A250XB43_9CHLO (Uncharacterized protein n=1 Tax=Chlamydomonas eustigma TaxID=1157962 RepID=A0A250XB43_9CHLO)

HSP 1 Score: 66.6 bits (161), Expect = 1.310e-7
Identity = 33/61 (54.10%), Postives = 42/61 (68.85%), Query Frame = 3 
Query:  918 WSVVGRAFFFLTGHHNQFSRLQYSAAFVGFDEFNFKIGGVMLFLNTFGTEILAALALPMLV 1100
            WS++G   FF TGH  +FS LQY++ F+GFDE  + + G ML  NTFG   L ALALP+LV
Sbjct:  774 WSMLGLMLFFCTGHFCEFSGLQYTSPFIGFDEMEWYVSGAMLTSNTFGAMALTALALPLLV 834
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BLAST of mRNA_H-elongata_contig52536.12558.1 vs. uniprot
Match: A0A2R5G5B3_9STRA (GPI ethanolamine phosphate transferase 2 n=1 Tax=Hondaea fermentalgiana TaxID=2315210 RepID=A0A2R5G5B3_9STRA)

HSP 1 Score: 57.8 bits (138), Expect = 2.870e-5
Identity = 26/52 (50.00%), Postives = 38/52 (73.08%), Query Frame = 3 
Query:  906 VALAWSVVGRAFFFLTGHHNQFSRLQYSAAFVGFDEFNFKIGGVMLFLNTFG 1061
            VAL WS+   ++FF TGH + F++L Y++ FVGFDE+ +  GG+M+  NTFG
Sbjct:  202 VALLWSMASMSYFFATGHESFFNKLHYASPFVGFDEYEYFRGGLMMGSNTFG 253
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BLAST of mRNA_H-elongata_contig52536.12558.1 vs. uniprot
Match: A0A2K3E1G7_CHLRE (GPI ethanolamine phosphate transferase 3 n=1 Tax=Chlamydomonas reinhardtii TaxID=3055 RepID=A0A2K3E1G7_CHLRE)

HSP 1 Score: 58.2 bits (139), Expect = 5.860e-5
Identity = 50/138 (36.23%), Postives = 70/138 (50.72%), Query Frame = 3 
Query:  780 VVVLLLGPGSPAIVLLVAAACGFALRGVSIAAGAG---------------------------VMVPLGAVALAWSVVGRAFFFLTGHHNQFSRLQYSAAFVGFDEFNFKIGGVMLFLNTFGTEILAALALPMLVAATV 1112
            V+ LLLGPG PA+VLL+A   G +L  + + A A                            V V   AV    S++G   FFLTGH  +FS LQY + FVGFD   +    ++ ++N+FG  +L A+ALP++ AA V
Sbjct: 1031 VLTLLLGPGGPAVVLLLAVQ-GASLLALLLHAAANTRWLHRQPKSNPTAPAGAATAPLPPAAVAVVAAAVGCLLSLLGGQAFFLTGHFCEFSGLQYDSPFVGFDHMTWAATPLLFWINSFGGLMLPAVALPLVAAAAV 1167
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BLAST of mRNA_H-elongata_contig52536.12558.1 vs. uniprot
Match: A0A061SPD4_9CHLO (Gpi ethanolamine phosphate transferase 3-like isoform x1 n=1 Tax=Tetraselmis sp. GSL018 TaxID=582737 RepID=A0A061SPD4_9CHLO)

HSP 1 Score: 57.4 bits (137), Expect = 7.580e-5
Identity = 27/57 (47.37%), Postives = 39/57 (68.42%), Query Frame = 3 
Query:  921 SVVGRAFFFLTGHHNQFSRLQYSAAFVGFDEFNFKIGGVMLFLNTFGTEILAALALP 1091
            +++G+  F+ TGH +QF+ LQY+A FVGFD F +   G +L  NTFG  +LAA  +P
Sbjct:  445 TLLGQHLFYCTGHTSQFTGLQYTAGFVGFDSFQYTRAGFLLAANTFGPHVLAAFLVP 501
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BLAST of mRNA_H-elongata_contig52536.12558.1 vs. uniprot
Match: A8I9K8_CHLRE (GPI ethanolamine phosphate transferase 3 n=1 Tax=Chlamydomonas reinhardtii TaxID=3055 RepID=A8I9K8_CHLRE)

HSP 1 Score: 57.4 bits (137), Expect = 9.130e-5
Identity = 41/111 (36.94%), Postives = 56/111 (50.45%), Query Frame = 3 
Query:  780 VVVLLLGPGSPAIVLLVAAACGFALRGVSIAAGAGVMVPLGAVALAWSVVGRAFFFLTGHHNQFSRLQYSAAFVGFDEFNFKIGGVMLFLNTFGTEILAALALPMLVAATV 1112
            V+ LLLGPG PA+VLL+AA                                   FFLTGH  +FS LQY + FVGFD   +    ++ ++N+FG  +L A+ALP++ AA V
Sbjct:  673 VLTLLLGPGGPAVVLLLAA-----------------------------------FFLTGHFCEFSGLQYDSPFVGFDHMTWAATPLLFWINSFGGLMLPAVALPLVAAAAV 748
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig52536.12558.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 7
Match NameE-valueIdentityDescription
D8LQQ0_ECTSI9.330e-8740.23GPI ethanolamine phosphate transferase 3 n=2 Tax=E... [more]
A0A4D9CYS9_9STRA6.740e-1472.88GPI ethanolamine phosphate transferase 3 n=2 Tax=M... [more]
A0A250XB43_9CHLO1.310e-754.10Uncharacterized protein n=1 Tax=Chlamydomonas eust... [more]
A0A2R5G5B3_9STRA2.870e-550.00GPI ethanolamine phosphate transferase 2 n=1 Tax=H... [more]
A0A2K3E1G7_CHLRE5.860e-536.23GPI ethanolamine phosphate transferase 3 n=1 Tax=C... [more]
A0A061SPD4_9CHLO7.580e-547.37Gpi ethanolamine phosphate transferase 3-like isof... [more]
A8I9K8_CHLRE9.130e-536.94GPI ethanolamine phosphate transferase 3 n=1 Tax=C... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig52536contigH-elongata_contig52536:296..2841 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score267.7
Seed ortholog evalue1.2e-68
Seed eggNOG ortholog2880.D8LQQ0
Preferred namePIGO
KEGG rclassRC00017
KEGG koko:K05288
KEGG ReactionR05923,R08107
KEGG Pathwayko00563,ko01100,map00563,map01100
GOsGO:0003674,GO:0003824,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005783,GO:0005789,GO:0006464,GO:0006497,GO:0006505,GO:0006506,GO:0006629,GO:0006643,GO:0006644,GO:0006650,GO:0006661,GO:0006664,GO:0006793,GO:0006796,GO:0006807,GO:0008150,GO:0008152,GO:0008610,GO:0008654,GO:0009058,GO:0009059,GO:0009247,GO:0009987,GO:0012505,GO:0016020,GO:0016021,GO:0016740,GO:0016772,GO:0016780,GO:0019538,GO:0019637,GO:0031224,GO:0031984,GO:0034645,GO:0036211,GO:0042157,GO:0042158,GO:0042175,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043412,GO:0044237,GO:0044238,GO:0044249,GO:0044255,GO:0044260,GO:0044267,GO:0044422,GO:0044424,GO:0044425,GO:0044432,GO:0044444,GO:0044446,GO:0044464,GO:0045017,GO:0046467,GO:0046474,GO:0046486,GO:0046488,GO:0051377,GO:0071704,GO:0090407,GO:0098827,GO:1901135,GO:1901137,GO:1901564,GO:1901566,GO:1901576,GO:1903509
EggNOG free text desc.mannose-ethanolamine phosphotransferase activity
EggNOG OGsCOG1524@1,KOG2126@2759
COG Functional cat.U
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001
Hectar predicted targeting categoryother localisation
Exons2
Model size2042
Cds size1590
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig52536.12558.1prot_H-elongata_contig52536.12558.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig52536 515..2608 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930858.056149-UTR-H-elongata_contig52536:295..5141622930858.056149-UTR-H-elongata_contig52536:295..514Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig52536 296..514 -
1691679600.251401-UTR-H-elongata_contig52536:295..5141691679600.251401-UTR-H-elongata_contig52536:295..514Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig52536 296..514 -
1622930858.1155472-UTR-H-elongata_contig52536:2608..28411622930858.1155472-UTR-H-elongata_contig52536:2608..2841Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig52536 2609..2841 -
1691679600.2845366-UTR-H-elongata_contig52536:2608..28411691679600.2845366-UTR-H-elongata_contig52536:2608..2841Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig52536 2609..2841 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930858.0788069-CDS-H-elongata_contig52536:514..13351622930858.0788069-CDS-H-elongata_contig52536:514..1335Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig52536 515..1335 -
1691679600.2678607-CDS-H-elongata_contig52536:514..13351691679600.2678607-CDS-H-elongata_contig52536:514..1335Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig52536 515..1335 -
1622930858.0970323-CDS-H-elongata_contig52536:1839..26081622930858.0970323-CDS-H-elongata_contig52536:1839..2608Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig52536 1840..2608 -
1691679600.2760332-CDS-H-elongata_contig52536:1839..26081691679600.2760332-CDS-H-elongata_contig52536:1839..2608Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig52536 1840..2608 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig52536.12558.1

>prot_H-elongata_contig52536.12558.1 ID=prot_H-elongata_contig52536.12558.1|Name=mRNA_H-elongata_contig52536.12558.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=530bp
MQAISLAATGAYWINDVAVEAREGAATAAAAVMRENARWSAYGGGLAALP
PLRLFLPRAVYFLCAVGFATALLLPVGRARARSKPQLWPRLRAQGRSEGG
TGDYPPQRSLSVQRGPPGEAASNAPARASSDSRSENRGDGFEFFGNTCSA
LGRASCGDPSVRLSYHHAAARTSAALVSHLLPVVVLLLGPGSPAIVLLVA
AACGFALRGVSIAAGAGVMVPLGAVALAWSVVGRAFFFLTGHHNQFSRLQ
YSAAFVGFDEFNFKIGGVMLFLNTFGTEILAALALPMLVAATVVVGSPAA
AAENDDGFDEKKKLQQTYQDPEEFSDKVADELRGKRRQRHRTMEEEEEDE
EDAQRSFLEQPRKEVWLEASQSRRGKSGATARRGKSTPSSLGGRHPSDDG
VWGAGVTTASGSRRWGDARETVPNPRSSASSAEEAFLSSMERISGLVLAL
SSARTLLSAANIAVQRGHLMLWAVFAPKFVFDATMQAVCGAAAVATWAVI
LVVHRGFHYGRVGGREREVPAGRYDMSSK*
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mRNA from alignment at H-elongata_contig52536:296..2841-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig52536.12558.1 ID=mRNA_H-elongata_contig52536.12558.1|Name=mRNA_H-elongata_contig52536.12558.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=2546bp|location=Sequence derived from alignment at H-elongata_contig52536:296..2841- (Himanthalia elongata Himel1 dioecious)
CGGCCGCCGCCCAGGAGTCGATCGCGGGGGTTGGGCTTTCTACGGAGGCC
GCGTTCGGTATCGCGCGCTCCCTCCTGCCCCTGCCCGCACTGTGGTGCCT
GTGCGCATCCGCGCGCGGCTTCGGCAACGTCGAGCGGGGCAAGCAACGAA
ACTCTGTCGACGTGACCGCAACTACCGCCGCCGTTTCCGCGGTGTTTGAC
TACTACCCTTTCGGGTTCCAGTTCCACGGCGTCATGCAGGCCATCAGCCT
CGCGGCAACCGGGGCGTACTGGATCAACGACGTCGCTGTAGAGGCACGGG
AAGGGGCTGCGACAGCGGCGGCGGCGGTAATGCGGGAAAATGCTCGATGG
TCGGCGTATGGTGGCGGCCTGGCGGCTCTCCCGCCCCTCCGGCTGTTCCT
CCCGCGAGCCGTCTACTTTTTATGCGCGGTAGGGTTTGCGACGGCGCTTC
TTCTCCCCGTGGGACGGGCGCGAGCACGTTCAAAGCCACAGCTGTGGCCG
CGATTGCGGGCACAGGGGCGTAGCGAGGGGGGAACGGGTGATTATCCTCC
CCAACGTTCGCTCTCCGTCCAGCGTGGGCCACCCGGCGAAGCAGCATCGA
ACGCGCCGGCGCGAGCCAGTAGCGACAGCCGCAGTGAAAACCGGGGCGAC
GGCTTTGAATTCTTCGGAAATACCTGCAGCGCTTTGGGGCGCGCCTCTTG
CGGAGATCCCTCGGTTCGGTTATCGTACCACCACGCGGCGGCTCGCACGT
CCGCTGCGCTCGTGTCGCACCTGCTTCCGGTCGTGGTGCTTCTCCTCGGG
CCGGGTTCCCCCGCGATCGTGCTGCTCGTGGCAGCGGCGTGCGGTTTTGC
GTTGAGAGGCGTTTCGATCGCGGCCGGGGCGGGGGTCATGGTTCCCCTTG
GGGCCGTGGCCCTGGCGTGGTCTGTCGTGGGGCGCGCCTTTTTTTTCTTG
ACCGGGCACCATAATCAATTCAGCAGGCTTCAATACTCGGCGGCATTTGT
GGGTAAGCGATAGAGAGAGGGGAAGCGACCATCATCATCATCAAATGATG
GTGTACTATATATATATATAGATGTTGAGTATTTGAATTCAGCGCAAGAT
AATCAGTAAGAGACACGTCGAAAGAACGGGGAGAATGGCCGATATATATT
TTTTAGTATTTCGCTTTTTCGATGTGAGAGGTTTAAAAAAGGACGATAAA
GTGTGTGAAGGTGAGAGATATGTGTATTTTTTGTTATTTCGTTCATCTGA
TTTAATCAAGAGTTAGACGAATTTTTTAAGCATCTCCCCTCCAAGGCGGC
GCCAAATTTCCCCCATCACACCATCGTTTGCTGGTTGTGTTGGTGTATGT
CCTCTCTTTCTCCGCTACCGTCGTGAGTGTGTGGCACGTCGTACATTTCC
TCTATTTGCCTGTCAGTTCGCTTTTTCCCACAAAAAAATAGAAGATCCGG
GGAAAATTATCCCCTCTTTTGAATTACTTCTGACTCTTGATACCTCCACC
GAGCAGGTTTCGACGAGTTCAACTTCAAGATCGGAGGCGTTATGCTCTTC
TTGAATACCTTCGGGACAGAGATCCTCGCAGCGCTGGCCTTGCCTATGCT
CGTCGCCGCGACGGTCGTCGTTGGCAGTCCTGCCGCTGCTGCTGAAAACG
ACGACGGCTTCGACGAGAAAAAGAAATTGCAACAAACATACCAGGATCCG
GAGGAATTTTCCGACAAAGTCGCCGACGAACTGCGAGGAAAGAGACGCCA
ACGACATAGGACAATGGAGGAAGAGGAAGAGGACGAGGAGGACGCGCAAC
GTTCTTTTCTCGAGCAGCCTAGAAAGGAAGTCTGGCTGGAAGCTTCCCAG
TCGAGAAGGGGCAAGAGCGGAGCGACTGCGAGAAGGGGGAAGAGCACGCC
ATCTTCGCTTGGCGGGCGCCATCCCAGCGACGATGGGGTTTGGGGAGCAG
GGGTGACCACGGCGTCGGGGAGTAGGAGATGGGGGGATGCACGCGAGACC
GTCCCCAACCCCCGCTCGTCAGCCTCTTCCGCGGAAGAGGCGTTTCTGTC
CTCGATGGAGCGCATCTCGGGGCTCGTCCTCGCCTTGAGCTCCGCGCGCA
CGCTCCTCTCGGCGGCCAACATCGCCGTACAGCGGGGGCACCTCATGCTC
TGGGCCGTCTTTGCCCCCAAGTTTGTCTTCGATGCGACCATGCAAGCCGT
CTGTGGGGCCGCAGCTGTGGCCACCTGGGCGGTGATCTTGGTCGTCCACA
GAGGTTTCCACTACGGTCGGGTCGGGGGTAGAGAGAGAGAGGTACCCGCT
GGTCGGTATGATATGAGTAGTAAGTGACGGGGTCTCCGCTGTCTTGTGCC
CTCTTGCTTACTGCTTGGTGGTGAATGGAACAAGCACGTCAATTATATAT
ATACCTTCACGGCACGGCTGCTAGGGTGTTGACGTATGGTCACCCGCCGA
AAGATGTACAAGCAAAAGTAGTCTTGAGATATAGACTATATTTATTACGC
CATCTTCGATCACCTAACTTTGATTCTTGATTAATTTAAAACATCC
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Coding sequence (CDS) from alignment at H-elongata_contig52536:296..2841-

>mRNA_H-elongata_contig52536.12558.1 ID=mRNA_H-elongata_contig52536.12558.1|Name=mRNA_H-elongata_contig52536.12558.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=3180bp|location=Sequence derived from alignment at H-elongata_contig52536:296..2841- (Himanthalia elongata Himel1 dioecious)
ATGCAGGCCATCAGCCTCGCGGCAACCGGGGCGTACTGGATCAACGACGT
CGCTGTAGAGGCACGGGAAGGGGCTGCGACAGCGGCGGCGGCGGTAATGC
GGGAAAATGCTCGATGGTCGGCGTATGGTGGCGGCCTGGCGGCTCTCCCG
CCCCTCCGGCTGTTCCTCCCGCGAGCCGTCTACTTTTTATGCGCGGTAGG
GTTTGCGACGGCGCTTCTTCTCCCCGTGGGACGGGCGCGAGCACGTTCAA
AGCCACAGCTGTGGCCGCGATTGCGGGCACAGGGGCGTAGCGAGGGGGGA
ACGGGTGATTATCCTCCCCAACGTTCGCTCTCCGTCCAGCGTGGGCCACC
CGGCGAAGCAGCATCGAACGCGCCGGCGCGAGCCAGTAGCGACAGCCGCA
GTGAAAACCGGGGCGACGGCTTTGAATTCTTCGGAAATACCTGCAGCGCT
TTGGGGCGCGCCTCTTGCGGAGATCCCTCGGTTCGGTTATCGTACCACCA
CGCGGCGGCTCGCACGTCCGCTGCGCTCGTGTCGCACCTGCTTCCGGTCG
TGGTGCTTCTCCTCGGGCCGGGTTCCCCCGCGATCGTGCTGCTCGTGGCA
GCGGCGTGCGGTTTTGCGTTGAGAGGCGTTTCGATCGCGGCCGGGGCGGG
GGTCATGGTTCCCCTTGGGGCCGTGGCCCTGGCGTGGTCTGTCGTGGGGC
GCGCCTTTTTTTTCTTGACCGGGCACCATAATCAATTCAGCAGGCTTCAA
TACTCGGCGGCATTTGTGGATGCAGGCCATCAGCCTCGCGGCAACCGGGG
CGTACTGGATCAACGACGTCGCTGTAGAGGCACGGGAAGGGGCTGCGACA
GCGGCGGCGGCGGTAATGCGGGAAAATGCTCGATGGTCGGCGTATGGTGG
CGGCCTGGCGGCTCTCCCGCCCCTCCGGCTGTTCCTCCCGCGAGCCGTCT
ACTTTTTATGCGCGGTAGGGTTTGCGACGGCGCTTCTTCTCCCCGTGGGA
CGGGCGCGAGCACGTTCAAAGCCACAGCTGTGGCCGCGATTGCGGGCACA
GGGGCGTAGCGAGGGGGGAACGGGTGATTATCCTCCCCAACGTTCGCTCT
CCGTCCAGCGTGGGCCACCCGGCGAAGCAGCATCGAACGCGCCGGCGCGA
GCCAGTAGCGACAGCCGCAGTGAAAACCGGGGCGACGGCTTTGAATTCTT
CGGAAATACCTGCAGCGCTTTGGGGCGCGCCTCTTGCGGAGATCCCTCGG
TTCGGTTATCGTACCACCACGCGGCGGCTCGCACGTCCGCTGCGCTCGTG
TCGCACCTGCTTCCGGTCGTGGTGCTTCTCCTCGGGCCGGGTTCCCCCGC
GATCGTGCTGCTCGTGGCAGCGGCGTGCGGTTTTGCGTTGAGAGGCGTTT
CGATCGCGGCCGGGGCGGGGGTCATGGTTCCCCTTGGGGCCGTGGCCCTG
GCGTGGTCTGTCGTGGGGCGCGCCTTTTTTTTCTTGACCGGGCACCATAA
TCAATTCAGCAGGCTTCAATACTCGGCGGCATTTGTGGGTTTCGACGAGT
TCAACTTCAAGATCGGAGGCGTTATGCTCTTCTTGAATACCTTCGGGACA
GAGATCCTCGCAGCGCTGGCCTTGCCTATGCTCGTCGCCGCGACGGTCGT
CGTTGGCAGTCCTGCCGCTGCTGCTGAAAACGACGACGGCTTCGACGAGA
AAAAGAAATTGCAACAAACATACCAGGATCCGGAGGAATTTTCCGACAAA
GTCGCCGACGAACTGCGAGGAAAGAGACGCCAACGACATAGGACAATGGA
GGAAGAGGAAGAGGACGAGGAGGACGCGCAACGTTCTTTTCTCGAGCAGC
CTAGAAAGGAAGTCTGGCTGGAAGCTTCCCAGTCGAGAAGGGGCAAGAGC
GGAGCGACTGCGAGAAGGGGGAAGAGCACGCCATCTTCGCTTGGCGGGCG
CCATCCCAGCGACGATGGGGTTTGGGGAGCAGGGGTGACCACGGCGTCGG
GGAGTAGGAGATGGGGGGATGCACGCGAGACCGTCCCCAACCCCCGCTCG
TCAGCCTCTTCCGCGGAAGAGGCGTTTCTGTCCTCGATGGAGCGCATCTC
GGGGCTCGTCCTCGCCTTGAGCTCCGCGCGCACGCTCCTCTCGGCGGCCA
ACATCGCCGTACAGCGGGGGCACCTCATGCTCTGGGCCGTCTTTGCCCCC
AAGTTTGTCTTCGATGCGACCATGCAAGCCGTCTGTGGGGCCGCAGCTGT
GGCCACCTGGGCGGTGATCTTGGTCGTCCACAGAGGTTTCCACTACGGTC
GGGTCGGGGGTAGAGAGAGAGAGGTACCCGCTGGTCGGTATGATATGAGT
AGTAAGTGAGTTTCGACGAGTTCAACTTCAAGATCGGAGGCGTTATGCTC
TTCTTGAATACCTTCGGGACAGAGATCCTCGCAGCGCTGGCCTTGCCTAT
GCTCGTCGCCGCGACGGTCGTCGTTGGCAGTCCTGCCGCTGCTGCTGAAA
ACGACGACGGCTTCGACGAGAAAAAGAAATTGCAACAAACATACCAGGAT
CCGGAGGAATTTTCCGACAAAGTCGCCGACGAACTGCGAGGAAAGAGACG
CCAACGACATAGGACAATGGAGGAAGAGGAAGAGGACGAGGAGGACGCGC
AACGTTCTTTTCTCGAGCAGCCTAGAAAGGAAGTCTGGCTGGAAGCTTCC
CAGTCGAGAAGGGGCAAGAGCGGAGCGACTGCGAGAAGGGGGAAGAGCAC
GCCATCTTCGCTTGGCGGGCGCCATCCCAGCGACGATGGGGTTTGGGGAG
CAGGGGTGACCACGGCGTCGGGGAGTAGGAGATGGGGGGATGCACGCGAG
ACCGTCCCCAACCCCCGCTCGTCAGCCTCTTCCGCGGAAGAGGCGTTTCT
GTCCTCGATGGAGCGCATCTCGGGGCTCGTCCTCGCCTTGAGCTCCGCGC
GCACGCTCCTCTCGGCGGCCAACATCGCCGTACAGCGGGGGCACCTCATG
CTCTGGGCCGTCTTTGCCCCCAAGTTTGTCTTCGATGCGACCATGCAAGC
CGTCTGTGGGGCCGCAGCTGTGGCCACCTGGGCGGTGATCTTGGTCGTCC
ACAGAGGTTTCCACTACGGTCGGGTCGGGGGTAGAGAGAGAGAGGTACCC
GCTGGTCGGTATGATATGAGTAGTAAGTGA
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