mRNA_H-elongata_contig74814.15161.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig74814.15161.1
Unique NamemRNA_H-elongata_contig74814.15161.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig74814.15161.1 vs. uniprot
Match: D7G5K3_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7G5K3_ECTSI)

HSP 1 Score: 211 bits (538), Expect = 6.160e-69
Identity = 102/118 (86.44%), Postives = 110/118 (93.22%), Query Frame = 1 
Query:    1 MRLLTHNVLRSPMKGVENGYPLRIEATDVQMLESEVKEEFLKQIAATLDWEALCKAASEVGLPKGSLPPALTPELLEDLSFIEALHRVLMDMHVVEGALICPETGRRFPITEGVPNLM 354
            MRLLTHNVLRSPMKGV  GYPLRIEATDVQM+ESEVKEEF+K +A TLDW ALCKAA+EVG+P+GSLPPALTPELLED SF+EALHRVLMDMHVVEG L+CPETGRRFPI EGVPNLM
Sbjct:    1 MRLLTHNVLRSPMKGVVEGYPLRIEATDVQMVESEVKEEFVKHMATTLDWGALCKAAAEVGMPEGSLPPALTPELLEDSSFLEALHRVLMDMHVVEGTLVCPETGRRFPIAEGVPNLM 118
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BLAST of mRNA_H-elongata_contig74814.15161.1 vs. uniprot
Match: A0A836CHT2_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CHT2_9STRA)

HSP 1 Score: 152 bits (383), Expect = 2.900e-45
Identity = 73/118 (61.86%), Postives = 93/118 (78.81%), Query Frame = 1 
Query:    1 MRLLTHNVLRSPMKGVENGYPLRIEATDVQMLESEVKEEFLKQIAATLDWEALCKAASEVGLPKGSLPPALTPELLEDLSFIEALHRVLMDMHVVEGALICPETGRRFPITEGVPNLM 354
            MRLLTHNVLR  +KGVE GYPL IEA++VQM ES  + +F+K IA TL+W AL +A  +VGLP+  LP A++  LL D +F+ ALHRVLMD+HVV GAL+CPE+GRRFPI  G+P++M
Sbjct:    1 MRLLTHNVLRCAIKGVEEGYPLSIEASEVQMEESPFRPDFVKHIAPTLNWPALRQAVKQVGLPEDMLPEAVSDALLGDEAFLRALHRVLMDVHVVAGALVCPESGRRFPIAHGIPDMM 118
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BLAST of mRNA_H-elongata_contig74814.15161.1 vs. uniprot
Match: A0A1Y1INK2_KLENI (Uncharacterized protein n=1 Tax=Klebsormidium nitens TaxID=105231 RepID=A0A1Y1INK2_KLENI)

HSP 1 Score: 125 bits (313), Expect = 1.040e-34
Identity = 59/118 (50.00%), Postives = 84/118 (71.19%), Query Frame = 1 
Query:    1 MRLLTHNVLRSPMKGVENGYPLRIEATDVQMLESEVKEEFLKQIAATLDWEALCKAASEVGLPKGSLPPALTPELLEDLSFIEALHRVLMDMHVVEGALICPETGRRFPITEGVPNLM 354
            MRLLTHN+L   +KGV  GYPL+IEAT  +  E++    FL+ I   LDW+AL +AA+ +GL +  LP  + P  LED  F+   H VL+++H+ EGAL+CPETGRRFP+++G+PN++
Sbjct:    1 MRLLTHNMLACNIKGVTKGYPLKIEATKTEEKETDFNAGFLRHIYPKLDWKALREAAASLGLEE--LPEQVEPSQLEDEDFLRRFHHVLLEIHLEEGALVCPETGRRFPVSKGIPNML 116
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BLAST of mRNA_H-elongata_contig74814.15161.1 vs. uniprot
Match: A0A2P6VL84_9CHLO (Multifunctional methyltransferase subunit TRM112 At1g22270 n=1 Tax=Micractinium conductrix TaxID=554055 RepID=A0A2P6VL84_9CHLO)

HSP 1 Score: 124 bits (311), Expect = 2.090e-34
Identity = 55/118 (46.61%), Postives = 87/118 (73.73%), Query Frame = 1 
Query:    1 MRLLTHNVLRSPMKGVENGYPLRIEATDVQMLESEVKEEFLKQIAATLDWEALCKAASEVGLPKGSLPPALTPELLEDLSFIEALHRVLMDMHVVEGALICPETGRRFPITEGVPNLM 354
            M+LLTHN+L   +KGV+NG+P +IEA  V++ E++ + +FL+ I   + W ALC+AA+ +G+    LP   T E+LED +F++  H  L+++H+ EGAL+CPETGR+FP++ G+PNL+
Sbjct:    1 MKLLTHNMLACHIKGVQNGFPFKIEAEKVEVQEADFEPDFLQHIFPRIQWAALCEAAAAMGVE--GLPAEATEEMLEDDAFMKKFHHALLEVHLQEGALVCPETGRKFPVSAGIPNLL 116
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BLAST of mRNA_H-elongata_contig74814.15161.1 vs. uniprot
Match: A0A803QXK8_CANSA (Uncharacterized protein n=3 Tax=Cannabis sativa TaxID=3483 RepID=A0A803QXK8_CANSA)

HSP 1 Score: 124 bits (310), Expect = 2.960e-34
Identity = 58/118 (49.15%), Postives = 82/118 (69.49%), Query Frame = 1 
Query:    1 MRLLTHNVLRSPMKGVENGYPLRIEATDVQMLESEVKEEFLKQIAATLDWEALCKAASEVGLPKGSLPPALTPELLEDLSFIEALHRVLMDMHVVEGALICPETGRRFPITEGVPNLM 354
            MRLLTHN+L S +KGV NG+PLRIE   V   E E+  +FLK + + +DW+AL +AA  +G  +  LP  + P +L+   F+   H  L+ +H+ EGALICPETGRRFP+T+G+PN++
Sbjct:    1 MRLLTHNMLSSNIKGVTNGFPLRIEVVKVVEKEVELNPDFLKSMFSKIDWKALVEAARSIGYTE--LPNEVDPSMLDSDDFLRRFHHALLQLHLEEGALICPETGRRFPVTKGIPNML 116
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BLAST of mRNA_H-elongata_contig74814.15161.1 vs. uniprot
Match: A0A2R5GJB0_9STRA (Multifunctional methyltransferase subunit TRM112-like protein n=1 Tax=Hondaea fermentalgiana TaxID=2315210 RepID=A0A2R5GJB0_9STRA)

HSP 1 Score: 123 bits (309), Expect = 4.210e-34
Identity = 59/118 (50.00%), Postives = 85/118 (72.03%), Query Frame = 1 
Query:    1 MRLLTHNVLRSPMKGVENGYPLRIEATDVQMLESEVKEEFLKQIAATLDWEALCKAASEVGLPKGSLPPALTPELLEDLSFIEALHRVLMDMHVVEGALICPETGRRFPITEGVPNLM 354
            M+LLTHN+L+  +KGV NGYPL+IE T  ++ E+E+   FL+ I   L+W     AA+++G  +  LP A+TPELLE   F++  HR L+++ VVEGALICPE+GR+F IT G+PN++
Sbjct:    1 MKLLTHNMLKCNVKGVNNGYPLKIEVTTKEIRETEMNPAFLQGIVEKLEWPVFRAAAAQLG--EEGLPEAVTPELLEQEDFLKKFHRALLEVVVVEGALICPESGRKFTITNGIPNML 116
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BLAST of mRNA_H-elongata_contig74814.15161.1 vs. uniprot
Match: A9S9D3_PHYPA (Predicted protein n=1 Tax=Physcomitrium patens TaxID=3218 RepID=A9S9D3_PHYPA)

HSP 1 Score: 122 bits (306), Expect = 1.200e-33
Identity = 58/118 (49.15%), Postives = 83/118 (70.34%), Query Frame = 1 
Query:    1 MRLLTHNVLRSPMKGVENGYPLRIEATDVQMLESEVKEEFLKQIAATLDWEALCKAASEVGLPKGSLPPALTPELLEDLSFIEALHRVLMDMHVVEGALICPETGRRFPITEGVPNLM 354
            MRLLTHN+L   +KGV  G+PL IE T ++  ESE+  +FL+ I   LDW+A  +AA  VG+   ++P  + P +L+D  F+   H  L+++H+ EGALICPETGRRFP+T+GVPN++
Sbjct:    1 MRLLTHNMLACNIKGVTKGFPLGIEHTRLETKESELNADFLRHIFPKLDWKAFHEAAQSVGV--NNIPDQVEPVMLDDDEFLRKFHHALLEVHLEEGALICPETGRRFPVTKGVPNML 116
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BLAST of mRNA_H-elongata_contig74814.15161.1 vs. uniprot
Match: A0A388LY57_CHABU (Uncharacterized protein n=1 Tax=Chara braunii TaxID=69332 RepID=A0A388LY57_CHABU)

HSP 1 Score: 122 bits (306), Expect = 1.200e-33
Identity = 57/118 (48.31%), Postives = 86/118 (72.88%), Query Frame = 1 
Query:    1 MRLLTHNVLRSPMKGVENGYPLRIEATDVQMLESEVKEEFLKQIAATLDWEALCKAASEVGLPKGSLPPALTPELLEDLSFIEALHRVLMDMHVVEGALICPETGRRFPITEGVPNLM 354
            MRLLTHN+L   +KGV  GYPL+IEA  ++  E+E   +FL++I   LDW+AL + A  + L +  LP    P +LED +F++ LH VL+++++ EGAL+CPETGRRFP+++G+PN++
Sbjct:    1 MRLLTHNMLTCNVKGVTKGYPLKIEAVRLEKKEAEFNPDFLRRIFPKLDWKALYETALSLDLDE--LPEQAEPSMLEDEAFLQKLHHVLLEVNLEEGALVCPETGRRFPVSKGIPNML 116
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BLAST of mRNA_H-elongata_contig74814.15161.1 vs. uniprot
Match: W7TQC2_9STRA (TRM112-like protein n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7TQC2_9STRA)

HSP 1 Score: 124 bits (310), Expect = 1.350e-33
Identity = 57/118 (48.31%), Postives = 87/118 (73.73%), Query Frame = 1 
Query:    1 MRLLTHNVLRSPMKGVENGYPLRIEATDVQMLESEVKEEFLKQIAATLDWEALCKAASEVGLPKGSLPPALTPELLEDLSFIEALHRVLMDMHVVEGALICPETGRRFPITEGVPNLM 354
            MRL+THN+L+S +KGV NG+PLRIEA  V++ E+E   EF++ +   +DW AL  A+ E+G+ +  LP  +      D SF++ +H++L+D+HV+EG+L+CPETGRRFPI  G+PN++
Sbjct:   52 MRLITHNMLKSNVKGVINGFPLRIEAEKVEVKETEFNSEFVQNLLPKVDWPALRGASKELGMDE--LPEEMDEANKTDDSFLQLVHKLLLDVHVLEGSLVCPETGRRFPINNGIPNML 167
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BLAST of mRNA_H-elongata_contig74814.15161.1 vs. uniprot
Match: A7RGF4_NEMVE (Multifunctional methyltransferase subunit TRM112-like protein n=1 Tax=Nematostella vectensis TaxID=45351 RepID=A7RGF4_NEMVE)

HSP 1 Score: 122 bits (305), Expect = 1.760e-33
Identity = 58/118 (49.15%), Postives = 83/118 (70.34%), Query Frame = 1 
Query:    1 MRLLTHNVLRSPMKGVENGYPLRIEATDVQMLESEVKEEFLKQIAATLDWEALCKAASEVGLPKGSLPPALTPELLEDLSFIEALHRVLMDMHVVEGALICPETGRRFPITEGVPNLM 354
            MRLLTHN+L+S +KGV+NG+PL IEA DVQ+ E +   EF+ ++   L+WEAL +AA ++G  +  LP  L      D  F++  H VL+++ V EG LICPETGR+FP+T G+PN++
Sbjct:    1 MRLLTHNMLKSHVKGVKNGFPLAIEAQDVQVCEVDFNPEFISRMIPKLEWEALVQAAQQIGHGQ-DLPTQLAEGYESDNDFLKKAHHVLLEVEVQEGQLICPETGRKFPVTNGIPNML 117
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig74814.15161.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7G5K3_ECTSI6.160e-6986.44Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
A0A836CHT2_9STRA2.900e-4561.86Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A1Y1INK2_KLENI1.040e-3450.00Uncharacterized protein n=1 Tax=Klebsormidium nite... [more]
A0A2P6VL84_9CHLO2.090e-3446.61Multifunctional methyltransferase subunit TRM112 A... [more]
A0A803QXK8_CANSA2.960e-3449.15Uncharacterized protein n=3 Tax=Cannabis sativa Ta... [more]
A0A2R5GJB0_9STRA4.210e-3450.00Multifunctional methyltransferase subunit TRM112-l... [more]
A9S9D3_PHYPA1.200e-3349.15Predicted protein n=1 Tax=Physcomitrium patens Tax... [more]
A0A388LY57_CHABU1.200e-3348.31Uncharacterized protein n=1 Tax=Chara braunii TaxI... [more]
W7TQC2_9STRA1.350e-3348.31TRM112-like protein n=1 Tax=Nannochloropsis gadita... [more]
A7RGF4_NEMVE1.760e-3349.15Multifunctional methyltransferase subunit TRM112-l... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig74814contigH-elongata_contig74814:93..1345 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score214.5
Seed ortholog evalue2.6e-53
Seed eggNOG ortholog2880.D7G5K3
Preferred nameTRMT112
KEGG koko:K15448
GOsGO:0000154,GO:0001510,GO:0003674,GO:0003824,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005737,GO:0005829,GO:0006139,GO:0006364,GO:0006396,GO:0006399,GO:0006400,GO:0006464,GO:0006479,GO:0006725,GO:0006807,GO:0008033,GO:0008150,GO:0008152,GO:0008168,GO:0008213,GO:0008276,GO:0009451,GO:0009893,GO:0009987,GO:0010467,GO:0010468,GO:0010604,GO:0010628,GO:0016070,GO:0016072,GO:0016740,GO:0016741,GO:0018364,GO:0019219,GO:0019222,GO:0019538,GO:0022613,GO:0030488,GO:0031167,GO:0031323,GO:0031325,GO:0031974,GO:0031981,GO:0032259,GO:0032991,GO:0034470,GO:0034641,GO:0034660,GO:0036211,GO:0036265,GO:0042254,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043412,GO:0043414,GO:0044085,GO:0044087,GO:0044089,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044422,GO:0044424,GO:0044428,GO:0044444,GO:0044446,GO:0044464,GO:0045935,GO:0046483,GO:0046982,GO:0046983,GO:0048471,GO:0048518,GO:0048522,GO:0050789,GO:0050794,GO:0051171,GO:0051173,GO:0051252,GO:0051254,GO:0060255,GO:0065007,GO:0070013,GO:0070475,GO:0070476,GO:0071704,GO:0071840,GO:0080090,GO:0090069,GO:0090070,GO:0090304,GO:0140096,GO:1901360,GO:1901564,GO:2000232,GO:2000234
EggNOG free text desc.peptidyl-glutamine methylation
EggNOG OGsKOG1088@1,KOG1088@2759
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03012,ko03016
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionUncharacterised protein family UPF0434/Trm112
Ec32 orthologEc-22_000960.1
Exons3
Model size357
Cds size357
Stop1
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931152.8198633-CDS-H-elongata_contig74814:92..1821622931152.8198633-CDS-H-elongata_contig74814:92..182Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig74814 93..182 +
1691679719.931935-CDS-H-elongata_contig74814:92..1821691679719.931935-CDS-H-elongata_contig74814:92..182Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig74814 93..182 +
1622931152.8391926-CDS-H-elongata_contig74814:704..7911622931152.8391926-CDS-H-elongata_contig74814:704..791Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig74814 705..791 +
1691679719.9505036-CDS-H-elongata_contig74814:704..7911691679719.9505036-CDS-H-elongata_contig74814:704..791Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig74814 705..791 +
1622931152.8588405-CDS-H-elongata_contig74814:1165..13451622931152.8588405-CDS-H-elongata_contig74814:1165..1345Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig74814 1166..1345 +
1691679719.962723-CDS-H-elongata_contig74814:1165..13451691679719.962723-CDS-H-elongata_contig74814:1165..1345Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig74814 1166..1345 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig74814.15161.1prot_H-elongata_contig74814.15161.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig74814 93..1345 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig74814.15161.1

>prot_H-elongata_contig74814.15161.1 ID=prot_H-elongata_contig74814.15161.1|Name=mRNA_H-elongata_contig74814.15161.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=119bp
MRLLTHNVLRSPMKGVENGYPLRIEATDVQMLESEVKEEFLKQIAATLDW
EALCKAASEVGLPKGSLPPALTPELLEDLSFIEALHRVLMDMHVVEGALI
CPETGRRFPITEGVPNLM*
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mRNA from alignment at H-elongata_contig74814:93..1345+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig74814.15161.1 ID=mRNA_H-elongata_contig74814.15161.1|Name=mRNA_H-elongata_contig74814.15161.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=1253bp|location=Sequence derived from alignment at H-elongata_contig74814:93..1345+ (Himanthalia elongata Himel1 dioecious)
ATGCGCCTACTCACTCACAATGTGCTTCGGTCACCGATGAAAGGGGTTGA
GAACGGTTATCCCTTGCGTATTGAAGCCACAGATGTCCAGGTCAGGGGCA
GCAGTCGTGACTTGTGACACAATTTTTTTTCTCCTGCCGAAATCATCAGC
ACCTCGCATATCAATCGTACAGTAGAGAGGCAGCACTATGGAATATGACG
AATCAGCCGCTGGTTGATCAACCCGCTGTTCTTTGGGAAGAGAAGCGTCT
TTGGCAAAAACATTATTGAGAAAAACCATATGCGTGCAGCGTGCAGCACA
CCTATGCCCTTGTGAAAATTCCTTGTTTGAATTCGCACGTGTAGCGGTTT
AGTCGTCTGGTGGTCCTCGTACAGTCCCGCCCGTTATCAGCAGTGGGCGG
TGGAGCAGATGCAGTTTCACGAGGGTGACACTCTCCTTGAAACTCACCTT
GGAGCACAACGTGATCGTGAACTTTCCCAAGTTCTCCTATTTTCGATACT
TTGACTCCCTGTACAACAGCGAAATGTAGCGTTCGGCACGATTCCTCGTT
TTACTTTGCTGATTGATATCAGTCTGATCATGGCGTCGCCCTCCGCCCTA
TGCTTCATGAAGATGTTGGAATCAGAAGTAAAGGAGGAATTTCTTAAGCA
AATCGCAGCGACGCTCGATTGGGAGGCATTGTGCAAAGCGGCATCCGAGG
TGAGCAGCATCCCCCTCCCCCTTAACTAATATTATCGCACATGTGACTGG
GTGATCATAGCATGCAGCCTTGGCGACAACACCAGCTGCTATGTGATAAG
CTTTCAGCCATAGTTGTTGTCGGTTGACAGGTTCTTGACATTGCGTCAAG
GCGTCGTTTTGGAAAATATACTAGTTTTAGTGTTAGGACGGGTTCAACCC
TTGTCAACCGACAACTCTACGTCTCAACTGTCCTCCGCGATAGACAAAAT
TATGCAGGCGTCATCCCGCTGTCTTCAAACAGGGGCATTGCTTAGCCACT
GCATTACAGTAGGGTTTCATCCTTCGTTCGTTGAAGAATCGCGAATCGCG
TTGCTCGGGATGTTGAGCTGCAGGTTGGCTTGCCTAAGGGTTCTCTCCCC
CCAGCCCTGACCCCGGAGCTGCTGGAAGACTTGTCTTTCATCGAGGCACT
ACATCGAGTCTTGATGGATATGCACGTCGTCGAGGGTGCCCTCATCTGTC
CGGAAACAGGAAGGCGCTTTCCTATAACAGAAGGCGTTCCGAACCTAATG
TGA
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Coding sequence (CDS) from alignment at H-elongata_contig74814:93..1345+

>mRNA_H-elongata_contig74814.15161.1 ID=mRNA_H-elongata_contig74814.15161.1|Name=mRNA_H-elongata_contig74814.15161.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=714bp|location=Sequence derived from alignment at H-elongata_contig74814:93..1345+ (Himanthalia elongata Himel1 dioecious)
ATGCGCCTACTCACTCACAATGTGCTTCGGTCACCGATGAAAGGGGTTGA
GAACGGTTATCCCTTGCGTATTGAAGCCACAGATGTCCAGATGCGCCTAC
TCACTCACAATGTGCTTCGGTCACCGATGAAAGGGGTTGAGAACGGTTAT
CCCTTGCGTATTGAAGCCACAGATGTCCAGATGTTGGAATCAGAAGTAAA
GGAGGAATTTCTTAAGCAAATCGCAGCGACGCTCGATTGGGAGGCATTGT
GCAAAGCGGCATCCGAGATGTTGGAATCAGAAGTAAAGGAGGAATTTCTT
AAGCAAATCGCAGCGACGCTCGATTGGGAGGCATTGTGCAAAGCGGCATC
CGAGGTTGGCTTGCCTAAGGGTTCTCTCCCCCCAGCCCTGACCCCGGAGC
TGCTGGAAGACTTGTCTTTCATCGAGGCACTACATCGAGTCTTGATGGAT
ATGCACGTCGTCGAGGGTGCCCTCATCTGTCCGGAAACAGGAAGGCGCTT
TCCTATAACAGAAGGCGTTCCGAACCTAATGTGAGTTGGCTTGCCTAAGG
GTTCTCTCCCCCCAGCCCTGACCCCGGAGCTGCTGGAAGACTTGTCTTTC
ATCGAGGCACTACATCGAGTCTTGATGGATATGCACGTCGTCGAGGGTGC
CCTCATCTGTCCGGAAACAGGAAGGCGCTTTCCTATAACAGAAGGCGTTC
CGAACCTAATGTGA
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